DOCSLIB.ORG

Suzhou Yacoo Science Co.,LTD No.128,Fangzhou Road,Suzhou Industral Park,China Tel:+86-512-87182055-871 Product List

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Suzhou Yacoo Science Co.,LTD No.128,Fangzhou Road,Suzhou Industral Park,China Tel:+86-512-87182055-871 Product List Suzhou Yacoo Science Co.,LTD No.128,Fangzhou Road,Suzhou Industral Park,China Tel:+86-512-87182055-871 Product List No. CAS No. Product Name Brief Name Category 1 77-86-1 Tris(hydroxymethyl)aminomethane Tris Buffer Tris(hydroxymethyl)aminomethane 2 1185-53-1 Tris-HCl Buffer hydrochloride 3 5704-04-1 Tris(hydroxymethyl)methylglycine Tricine Buffer N-[Tris(hydroxymethyl)methyl]-3- 4 29915-38-6 TAPS Buffer aminopropanesulfonic acid 4-(2-Hydroxyethyl)piperazine-1- 5 7365-45-9 HEPES Buffer ethanesulfonic acid 6 75277-39-3 HEPES, Sodium salt HEPES-Na Buffer 7 1132-61-2 MOPS MOPS Buffer 8 71119-22-7 MOPS sodium salt MOPS-Na Buffer 3-(N-Morpholino)-2- 9 68399-77-9 MOPSO Buffer Hydroxypropanesulfonic Acid 10 79803-73-9 MOPSO sodium salt MOPSO-Na Buffer 11 4432-31-9 MES MES Buffer 12 71119-23-8 MES sodium salt MES-Na Buffer 2-Morpholinoethanesulfonic acid, 13 145224-94-8 MES·H2O Buffer monohydrate N-Cyclohexyl-3-aminopropanesulfonic 14 1135-40-6 CAPS Buffer acid 15 103-47-9 2-(Cyclohexylamino)ethanesulfonic acid CHES Buffer 3-(Cyclohexylamino)-2-hydroxy-1- 16 73463-39-5 CAPSO Buffer propanesulfonic acid 3-Cyclohexylamino-2- 17 102601-34-3 CAPSO-Na Buffer hydroxypropanesulfonic acid sodium salt 3-((3- 18 75621-03-3 Cholamidopropyl)dimethylammonium)- CHAPS Buffer 1-propanesulfonate 19 150-25-4 N,N-Bis(2-Hydroxyethyl)Glycine Bicine Buffer 20 6976-37-0 BIS-TRIS Bis-Tris Buffer 1，3- 21 64431-96-5 bis(tris(hydroxymethyl)methylamino)pro Bis-Tris propane Buffer pane 22 124763-51-5 Bis-Tris Hydrochloride Bis-Tris HCl Buffer 23 5625-37-6 Piperazine-1,4-bisethanesulfonic acid PIPES Buffer 24 76836-02-7 PIPES-2Na PIPES-2Na Buffer N-(2-Acetamido)-2-Aminoethanesulfonic 25 7365-82-4 ACES Buffer acid 26 26239-55-4 N-(2-acetamido)iminodiacetic acid ADA Buffer 3-((3- 27 75621-03-3 Cholamidopropyl)dimethylammonium)- CHAPS Buffer 1-propanesulfonate 4-Amino-3-hydrazino-1,2,4-triazol-5- Chromogenic 28 1750-12-5 AHMT thiol Substrate Chromogenic 29 1866-15-5 Acetylthiocholine iodide Acetylthiocholine iodide Substrate 5-Bromo-4-chloro-3-indolyl phosphate p- Chromogenic 30 6578-06-9 BCIP toluidine salt Substrate Chromogenic 31 4264-83-9 Disodium 4-nitrophenylphosphate PNPP Substrate S-Butyrylthiocholine Chromogenic 32 1866-16-6 S-Butyrylthiocholine iodide iodide Substrate Chromogenic 33 69-78-3 5,5'-Dithiobis(2-nitrobenzoic acid) DTNB Substrate Chromogenic 34 620-45-1 2,6-Dichloroindophenol sodium salt DCIP Substrate 2,4- 2,4-Dichlorobenzenediazonium Chromogenic 35 21872-70-8 Dichlorobenzenediazoni tetrafluoroborate Substrate um tetrafluoroborate γ-L-Glutamyl-3-carboxy-4-nitroanilide, Chromogenic 36 63699-78-5 γ-GT monoammonium salt Substrate Chromogenic 37 38894-11-0 MBTH MBTH Substrate Chromogenic 38 298-83-9 Nitrotetrazolium blue chloride NBT Substrate Chromogenic 39 521-31-3 3-Aminophthalhydrazide Luminol Substrate Chromogenic 40 20666-12-0 Luminol monosodium salt Luminol-Na Substrate 2-hydroxy-3,5-dichlorobenzenesulfonic Chromogenic 41 54970-72-8 DHBS acid,sodium salt Substrate Chromogenic 42 53279-72-4 3-Hydroxy-2,4,6-triiodobenzoic acid HTBA Substrate Chromogenic 43 27848-80-2 Lithium L-lactate Lithium L-lactate Substrate Chromogenic 44 54827-17-7 3,3',5,5'-Tetramethylbenzidine TMB Substrate Chromogenic 45 207738-08-7 TMB dihydrochloride TMB-HCl Substrate 5-Bromo-4-chloro-3- Chromogenic 46 3252-36-6 5-Bromo-4-chloro-3-indolyl acetate indolyl acetate Substrate Ethylene glycol-bis(2-aminoethyl ether)- Chromogenic 47 67-42-5 EGTA N,N,N',N'-tetraacetic acid Substrate 3-(N-ethyl-methylanilino)-2-hydroxy Chromogenic 48 82692-93-1 TOOS propane sulfonic acid, Sodium Salt Substrate N-ethyl-N-(3-sulfopropyl)-m-toluidine, Chromogenic 49 40567-80-4 TOPS sodium salt Substrate Chromogenic 50 193884-53-6 Lumigen APS 5 APS-5 Substrate 51 65-22-5 Pyridoxal hydrochloride Pyridoxal hydrochloride pharmaceutical 52 367-93-1 Isopropyl-beta-D-thiogalactopyranoside IPTG pharmaceutical TETRAKIS(DECYL)AMMONIUM TETRAKIS(DECYL)A 53 14937-42-9 pharmaceutical BROMIDE MMONIUM BROMIDE POLYQUATERNIUM- 54 75345-27-6 POLYQUATERNIUM-1 pharmaceutical 1 55 20846-91-7 N,N'-1,2-ethanediylbis-1-Aspartic acid EDDS pharmaceutical 56 329-98-6 Phenylmethanesulfonyl fluoride PMSF others N,N'- 57 110-26-9 N,N'-Methylenebisacrylamide others Methylenebisacrylamide 2,2’-Biquinoline-4,4’-dicarboxylic acid 58 207124-63-8 BCA-2K others dipotassium salt trihydrate 2,2'-Biquinoline-4,4-dicarboxylic acid 59 979-88-4 BCA-2Na others disodium salt 60 9048-46-8 Bovine albumin BSA others 61 298-93-1 Thiazolyl Blue MTT others Tris(2-carboxyethyl)phosphine 62 51805-45-9 TCEP-Hcl others hydrochloride 8-Anilino-1-naphthalenesulfonic acid 63 28836-03-5 ANS-NH4 others ammonium salt 64 50-01-1 Guanidine hydrochloride Guanidine hcl others 65 593-84-0 Guanidine isothiocyanate Guanidine thiocyanate others Electronic 66 28804-46-8 Dichloro-[2,2]-paracyclophane Parylene C chemical Electronic 67 1633-22-3 Di-p-xylylene Parylene N chemical Electronic 68 126213-50-1 3,4-Ethylenedioxythiophene EDOT chemical Electronic 69 28210-41-5 Polystyrene sulfonic acid PSS chemical Electronic 70 3010-96-6 2,2,4,4-Tetramethyl-1,3-cyclobutanediol CBDO chemical.
Load more

Recommended publications
  • Thermodynamic Quantities for the Ionization Reactions of Buffers
    Thermodynamic Quantities for the Ionization Reactions of Buffers Robert N. Goldberg,a… Nand Kishore,b… and Rebecca M. Lennen Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 ͑Received 21 June 2001; accepted 16 September 2001; published 24 April 2002͒ This review contains selected values of thermodynamic quantities for the aqueous ionization reactions of 64 buffers, many of which are used in biological research. Since the aim is to be able to predict values of the ionization constant at temperatures not too far from ambient, the thermodynamic quantities which are tabulated are the pK, standard ⌬ ؠ ⌬ molar Gibbs energy rG , standard molar enthalpy rH°, and standard molar heat ca- ؠ ⌬ pacity change rC p for each of the ionization reactions at the temperature T ϭ298.15 K and the pressure pϭ0.1 MPa. The standard state is the hypothetical ideal solution of unit molality. The chemical name͑s͒ and CAS registry number, structure, empirical formula, and molecular weight are given for each buffer considered herein. The selection of the values of the thermodynamic quantities for each buffer is discussed. © 2002 by the U.S. Secretary of Commerce on behalf of the United States. All rights reserved. Key words: buffers; enthalpy; equilibrium constant; evaluated data; Gibbs free energy; heat capacity; ionization; pK; thermodynamic properties. Contents 7.19. CAPSO.............................. 263 7.20. Carbonate............................ 264 7.21. CHES............................... 264 1. Introduction................................ 232 7.22. Citrate............................... 265 2. Thermodynamic Background.................. 233 7.23. L-Cysteine............................ 268 3. Presentation of Data......................... 234 7.24. Diethanolamine........................ 270 4. Evaluation of Data. ........................ 235 7.25. Diglycolate..........................
    [Show full text]
  • Buffers a Guide for the Preparation and Use of Buffers in Biological Systems Calbiochem® Buffers a Guide for the Preparation and Use of Buffers in Biological Systems
    Buffers A guide for the preparation and use of buffers in biological systems Calbiochem® Buffers A guide for the preparation and use of buffers in biological systems Chandra Mohan, Ph.D. EMD, San Diego, California © EMD, an affiliate of Merck KGaA, Darmstadt, Germany. All rights reserved. A word to our valued customers We are pleased to present to you the newest edition of Buffers: A Guide for the Preparation and Use of Buffers in Biological Systems. This practical resource has been especially revamped for use by researchers in the biological sciences. This publication is a part of our continuing commitment to provide useful product information and exceptional service to you, our customers. You will find this booklet a highly useful resource, whether you are just beginning your research work or training the newest researchers in your laboratory. Over the past several years, EMD Biosciences has clearly emerged as a world leader in providing highly innovative products for your research needs in Signal Transduction, including the areas of Cancer Biology, Alzheimer’s Disease, Diabetes, Hypertension, Inflammation, and Apoptosis. Please call us today for a free copy of our LATEST Catalog that includes tools for signal transduction and life science research. If you have used our products in the past, we thank you for your support and confidence in our products, and if you are just beginning your research career, please call us and give us the opportunity to demonstrate our exceptional customer and technical service. Corrine Fetherston Sr. Director, Marketing ii Table of Contents: Why does Calbiochem® Biochemicals Publish a Booklet on Buffers? .
    [Show full text]
  • Biological Buffers and Ultra Pure Reagents
    Biological Buffers and Ultra Pure Reagents Are MP Buffers in your corner? One Call. One Source. A World of Ultra Pure Biochemicals. www.mpbio.com Theoretical Considerations Since buffers are essential for controlling the pH in many Since, under equilibrium conditions, the rates of dissociation and biological and biochemical reactions, it is important to have a association must be equal, they may be expressed as: basic understanding of how buffers control the hydrogen ion concentration. Although a lengthy, detailed discussion is impractical, + - k1 (HAc) = k2 (H ) (Ac ) some explanation of the buffering phenomena is important. Or Let us begin with a discussion of the equilibrium constant (K) for + - weak acids and bases. Acids and bases which do not completely k1 = (H ) (Ac ) dissociate in solution, but instead exist as an equilibrium mixture of k (HAc) undissociated and dissociated species, are termed weak acids and 2 bases. The most common example of a weak acid is acetic acid. If we now let k1/k2 = Ka , the equilibrium constant, the equilibrium In solution, acetic acid exists as an equilibrium mixture of acetate expression becomes: ions, hydrogen ions, and undissociated acetic acid. The equilibrium between these species may be expressed as follows: + - Ka = (H ) (Ac ) k1 (HAc) + - HAc ⇌ H + Ac which may be rearranged to express the hydrogen ion concentration k2 in terms of the equilibrium constant and the concentrations of undissociated acetic acid and acetate ions as follows: where k1 is the dissociation rate constant of acetic acid to acetate and hydrogen ions and k is the association rate constant of the ion 2 (H+) = K (HAc) species to form acetic acid.
    [Show full text]
  • Standard Abbreviations
    Journal of CancerJCP Prevention Standard Abbreviations Journal of Cancer Prevention provides a list of standard abbreviations. Standard Abbreviations are defined as those that may be used without explanation (e.g., DNA). Abbreviations not on the Standard Abbreviations list should be spelled out at first mention in both the abstract and the text. Abbreviations should not be used in titles; however, running titles may carry abbreviations for brevity. ▌Abbreviations monophosphate ADP, dADP ‌adenosine diphosphate, deoxyadenosine IR infrared diphosphate ITP, dITP ‌inosine triphosphate, deoxyinosine AMP, dAMP ‌adenosine monophosphate, deoxyadenosine triphosphate monophosphate LOH loss of heterozygosity ANOVA analysis of variance MDR multiple drug resistance AP-1 activator protein-1 MHC major histocompatibility complex ATP, dATP ‌adenosine triphosphate, deoxyadenosine MRI magnetic resonance imaging trip hosphate mRNA messenger RNA bp base pair(s) MTS ‌3-(4,5-dimethylthiazol-2-yl)-5-(3- CDP, dCDP ‌cytidine diphosphate, deoxycytidine diphosphate carboxymethoxyphenyl)-2-(4-sulfophenyl)- CMP, dCMP ‌cytidine monophosphate, deoxycytidine mono- 2H-tetrazolium phosphate mTOR mammalian target of rapamycin CNBr cyanogen bromide MTT ‌3-(4,5-Dimethylthiazol-2-yl)-2,5- cDNA complementary DNA diphenyltetrazolium bromide CoA coenzyme A NAD, NADH ‌nicotinamide adenine dinucleotide, reduced COOH a functional group consisting of a carbonyl and nicotinamide adenine dinucleotide a hydroxyl, which has the formula –C(=O)OH, NADP, NADPH ‌nicotinamide adnine dinucleotide
    [Show full text]
  • Nickel Chelating Resin Spin Columns
    327PR G-Biosciences, St Louis, MO. USA ♦ 1-800-628-7730 ♦ 1-314-991-6034 ♦ [email protected] A Geno Technology, Inc. (USA) brand name Cobalt Chelating Resin Spin Columns INTRODUCTION Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Cobalt Chelating Resin is specifically designed for the purification of recombinant proteins fused to the 6X histidine (6XHis) tag. ITEM(S) SUPPLIED Cat. # Description Total Column Volume Size 786-454 Cobalt Chelating Resin, 0.2ml Spin Column 1ml 25 columns 786-455 Cobalt Chelating Resin, 1ml Spin Column 8ml 5 columns 786-456 Cobalt Chelating Resin, 3ml Spin Column 22ml 5 columns *Cobalt Chelating Resin is supplied as a 50% slurry in 20% ethanol STORAGE CONDITIONS It is shipped at ambient temperature. Upon arrival, store it refrigerated at 4°C, DO NOT FREEZE. This product is stable for 1 year at 4°C. SPECIFICATIONS Ligand Density: 20-40μmoles Co2+/ ml resin Binding Capacity: >50mg/ml resin. We have demonstrated binding of >100mg of a 50kDa 6X His tagged proteins to a ml of resin Bead Structure: 6% cross-linked agarose IMPORTANT INFORMATION • The purity and yield of the recombinant fusion protein is dependent of the protein’s confirmation, solubility and expression levels. We recommend optimizing and performing small scale preparations to estimate expression and solubility levels. • Avoid EDTA containing protease inhibitor cocktails, we recommend our Recom ProteaseArrest™ (Cat. # 786-376, 786-436) for inhibiting proteases during the purification of recombinant proteins.
    [Show full text]
  • Catalysis Science & Technology
    Catalysis Science & Technology View Article Online PAPER View Journal | View Issue Morpholine-based buffers activate aerobic via Cite this: Catal. Sci. Technol.,2019, photobiocatalysis spin correlated ion pair 9,1365 formation† Leticia C. P. Gonçalves, *a Hamid R. Mansouri, a Erick L. Bastos, b Mohamed Abdellah,cd Bruna S. Fadiga,bc Jacinto Sá, ce Florian Rudroff a and Marko D. Mihovilovic a The use of enzymes for synthetic applications is a powerful and environmentally-benign approach to in- crease molecular complexity. Oxidoreductases selectively introduce oxygen and hydrogen atoms into myr- iad substrates, catalyzing the synthesis of chemical and pharmaceutical building blocks for chemical pro- duction. However, broader application of this class of enzymes is limited by the requirements of expensive cofactors and low operational stability. Herein, we show that morpholine-based buffers, especially 3-(N- morpholino)propanesulfonic acid (MOPS), promote photoinduced flavoenzyme-catalyzed asymmetric re- Creative Commons Attribution 3.0 Unported Licence. Received 14th December 2018, dox transformations by regenerating the flavin cofactor via sacrificial electron donation and by increasing Accepted 8th February 2019 the operational stability of flavin-dependent oxidoreductases. The stabilization of the active forms of flavin by MOPS via formation of the spin correlated ion pair 3ijflavin˙−–MOPS˙+] ensemble reduces the formation DOI: 10.1039/c8cy02524j of hydrogen peroxide, circumventing the oxygen dilemma under aerobic conditions detrimental
    [Show full text]
  • Specialty Intermediates 2020 Product Guide
    Specialty Intermediates 2020 Product Guide Creating Customer Success® Maroon Group - Specialty Intermediates A Acetic Anhydride D Acetyl Acetone (2,4-Pentanedione) ● Dibasic Ester (DBE) Acetyl Tributyl Citrate (ATBC) ● Dibutyl Phthalate (DBP) 2-Acrylamido-2-Methyl-1-Propanesulfonic Acid Dicyandiamide ● Sodium Salt (ATBS) Diethanolamine (DEA) Acrylic Acid, Glacial (GAA) ● 1,2-Dimethoxybenzene Adipic Acid, Tech Grade ● Diethylaminoethyl Methacrylate (DEAEMA) Allyl Methacrylate (AMA) Diethylene Glycol Dimethyl Ether (Diglyme) Alpha Methyl Styrene (AMS) Dimethyl Acetamide (DMAC) Alpha Methyl Benzylamine (AMB) Dimethyl Terephthalate (DMT) ● Aluminum Acetylacetonate (AAA) Dimethylaminoethyl Methacrylate (DMAEMA) Ammonium Acetate ● Diosgenin Ammonium Oxalate, Monohydrate Dipentaerythritol, Micronized ● Ammonium Persulfate (APS) ● Dipentaerythritol 85% ● Ammonium Polyphosphate (APP) ● Dipentaerythritol 90% ● Ammonium Thiocyanate ● DiPotassium Phosphate (DKP) ● Anisole Divinyl Glycol (DVG) Antioxidant-1425 DL-Serine Ascorbic Acid 6-Palmitate Azelaic Acid E Epichlorohydrin (ECH) ● B Erythorbic Acid, Food Grade Barium Hydroxide, Monohydrate ● Ethyl Acetate (ETAC) Barium Hydroxide, Octahydrate ● Ethyl Acrylate (EA) ● Benzoic Acid, Food/Pharma Grade ● 2-Ethylhexyl Acrylate (2EHA) ● Benzoic Acid, Tech Grade ● Ethylene Glycol Dimethacrylate (EGDMA) Benzyl Alcohol ● Bisphenol A (BPA) F Boron Nitride Formaldehyde 37%, ACS Grade ● 1,4-Butanediol Diglycidyl Ether (1,4-BDE) Ferric Ammonium Citrate, Green ● Butyl Acetate (BUTAC) Ferric Ammonium Citrate, Reddish
    [Show full text]
  • Protein Gel Electrophoresis Technical Handbook Separate Transfer Detect 2
    Western blotting Protein gel electrophoresis technical handbook separate transfer detect 2 Comprehensive solutions designed to drive your success Protein gel electrophoresis is a simple way to separate proteins prior to downstream detection or analysis, and is a critical step in most workflows that isolate, identify, and characterize proteins. We offer a complete array of products to support rapid, reliable protein electrophoresis for a variety of applications, whether it is the first or last step in your workflow. Our portfolio of high-quality protein electrophoresis products unites gels, stains, molecular weight markers, running buffers, and blotting products for your experiments. For a complete listing of all available products and more, visit thermofisher.com/separate For orderingordering informationinformation referrefer to pagepage XX.XX ForForqr quickquickrk referencereferencee on the protocolprotocol pleasepleasere referrefertr totopo pagepage XX. Prepare samples Choose the electrophoresis Select precast gel and select buffers Select the standard chamber system and power supply Run the gel Stain the gel Post stain 3 Contents Electrophoresis overview 4 Select precast gel Gel selection guide 8 Gels 10 Prepare samples and select buffers Sample prep kits 26 Buffers and reagents 28 Buffers and reagents selection guide 29 Select the standard Protein ladders 34 Protein standards selection guide 36 Choose the electrophoresis chamber system and power supply Electrophoresis chamber systems 50 Electrophoresis chamber system selection guide 51
    [Show full text]
  • Biological Buffers
    AppliCations No.2 Biological Buffers Many biochemical processes are markedly impaired by even small changes in the con centrations of free H+ ions. It is therefore usually necessary to stabilise the H+ concentration in vitro by adding a suitable buffer to the medium, without, however, affecting the functioning of the system under investigation. A buffer keeps the pH of a solution constant by taking up protons that are released during reactions, or by r eleasing protons when they are consumed by reactions. This handout summarizes the most commonly used buffer substances and respec- tive physical and chemical properties. Keywords Practical Tips – Preparing Buffer Solutions chemical properties Recommendations for the setting of the pH value of a buffer and storage conditions usefull pH range 1. Temperature Depending on the buffer substance, its pH may vary with temperature. It is therefore advisable, as far as possible, to set the pH buffer preparation at the working temperature to be used for the investigation. For instance the physiological pH value for most mammalian cells at 37°C is between 7.0 and 7.5. The temperature dependence of a buffer system is expressed as d(pKa)/dT, which describes the change of the pKa at an increase of temperature by 1°C. 2. Titration (i) Generally, the pH value is set using NaOH/KOH or HCl. Slow addition of a strong acid or base whilst stirring vigorously avoids local high concentrations of H+ or OH– ions. If this is not done, the buffer substances may undergo chemical changes that inactivate them or modify them so that they have an inhibitory action (Ellis & Morrison 1982).
    [Show full text]
  • Evidence-Based Guidelines for Controlling Ph in Mammalian Live-Cell Culture Systems
    ARTICLE https://doi.org/10.1038/s42003-019-0393-7 OPEN Evidence-based guidelines for controlling pH in mammalian live-cell culture systems Johanna Michl1, Kyung Chan Park 1 & Pawel Swietach 1 1234567890():,; A fundamental variable in culture medium is its pH, which must be controlled by an appropriately formulated buffering regime, since biological processes are exquisitely sensitive to acid–base chemistry. Although awareness of the importance of pH is fostered early in the training of researchers, there are no consensus guidelines for best practice in managing pH in cell cultures, and reporting standards relating to pH are typically inadequate. Furthermore, many laboratories adopt bespoke approaches to controlling pH, some of which inadvertently produce artefacts that increase noise, compromise reproducibility or lead to the mis- interpretation of data. Here, we use real-time measurements of medium pH and intracellular pH under live-cell culture conditions to describe the effects of various buffering regimes, − including physiological CO2/HCO3 and non-volatile buffers (e.g. HEPES). We highlight those cases that result in poor control, non-intuitive outcomes and erroneous inferences. To improve data reproducibility, we propose guidelines for controlling pH in culture systems. 1 Department of Physiology, Anatomy and Genetics, University of Oxford, OX1 3PT Oxford, UK. Correspondence and requests for materials should be addressed to P.S. (email: [email protected]) COMMUNICATIONS BIOLOGY | (2019) 2:144 | https://doi.org/10.1038/s42003-019-0393-7 | www.nature.com/commsbio 1 ARTICLE COMMUNICATIONS BIOLOGY | https://doi.org/10.1038/s42003-019-0393-7 iomedical laboratories routinely perform cell culture to Results Bproduce a cellular environment that is precisely defined, Monitoring culture medium pH under incubation.
    [Show full text]
  • Download Author Version (PDF)
    RSC Advances This is an Accepted Manuscript, which has been through the Royal Society of Chemistry peer review process and has been accepted for publication. Accepted Manuscripts are published online shortly after acceptance, before technical editing, formatting and proof reading. Using this free service, authors can make their results available to the community, in citable form, before we publish the edited article. This Accepted Manuscript will be replaced by the edited, formatted and paginated article as soon as this is available. You can find more information about Accepted Manuscripts in the Information for Authors. Please note that technical editing may introduce minor changes to the text and/or graphics, which may alter content. The journal’s standard Terms & Conditions and the Ethical guidelines still apply. In no event shall the Royal Society of Chemistry be held responsible for any errors or omissions in this Accepted Manuscript or any consequences arising from the use of any information it contains. www.rsc.org/advances Page 1 of 7 RSC Advances RSC Publishing COMMUNICATION Synthesis of choline sulfonate buffers and their effect on cytochrome c dissolution and Cite this: DOI: 10.1039/x0xx00000x oxidation state Sara C. Matias a, Ângelo Rocha a, Raquel Teixeira a, Luis J.P. Fonseca a, b, a* Received 00th January 2012, Nuno M.T. Lourenço Accepted 00th January 2012 DOI: 10.1039/x0xx00000x Seven choline sulfonates with buffering properties were prepared in good yields (74-94%) and www.rsc.org/ high purity by reacting choline hydroxide with different Good’s buffers. Choline sulfonate buffers containing hydroxyl group-rich cations appeared to be liquid at room temperature.
    [Show full text]
  • Buffer Compatibility with Nano DSC
    TA Instruments – Technical Note __________________________________________________________________________________ Buffer Compatibility with Nano DSC Colette Quinn, Ph.D. Microcalorimetry, TA Instruments – Waters LLC, 890 West 410 North, Lindon, UT 84042, USA Introduction When using differential scanning calorimetry (DSC), a high quality buffer scan is imperative in order to accurately determine the partial specific heat capacity of a protein sample or when working with low concentrations of biological macromolecules. The reproducibility of the scans in a Nano DSC instrument is a desirable feature and well documented in the application note: High-throughput DSC: A Comparison of the TA Instruments Nano DSC Autosampler SystemTM with the GE Healthcare VP-Capillary DSCTM. One of the key preparatory steps that will ensure the collection of a good background buffer scan is to condition the DSC sample and reference cells with buffer. Proper cell conditioning is accomplished by filling both cells with buffer and then scanning to the upper temperature limit anticipated for scans that will be collected with test samples in this buffer. During the initial conditioning heat scan, the surface of the cells becomes populated with ions from the buffer. These ions physically adsorb to the surface of the cells. The cells will remain conditioned as long as they are either rinsed with water or cleaned with a dilute detergent, such as 1% Contrad®, followed by a water rinse. Because most proteins are removed using these gentle conditions and the conditioning will be left intact, the cell does not need to be pre-conditioned prior to every sample scan. However, if after a sample scan, a particular biological macromolecule can only be cleaned from the sample cell by the thorough cleaning conditions of 4 M sodium hydroxide followed by 50% formic acid, as recommended in the Nano DSC Getting Started Guide, then the cells will need to be reconditioned.
    [Show full text]