Step by Step Guide to Creating Custom Monoclonal Antibodies
Generation of antibody producing Is the mouse producing your 1 B Cells in mice 2 antibodies of interest?
Antibodies
Epitopes
Blood Antibodies Sample of Interest
Antigens Antigen Host Animal Host Animal (Mouse) (Mouse)
A mouse is immunized against the target antigen of interest Test bleeds are collected and analyzed for the presence of using, antigenic peptides, proteins or biological material NO antibodies targeting the antigen of interest. that is prepared with a suitable adjuvant and used under a specified immunization protocol. YES
Dissociation of mouse spleen tissue 3 releasing resident B cells and mixed 4 Addition of Polyethylene Glycol (PEG) with a specific ratio of immortal mouse myeloma cells B Cell Myeloma Cell Myeloma Cells
Hybridomas