AK4 Antibody Cat. No.: 15-003
AK4 Antibody
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug AK4 antibody (15-003). Immunofluorescence analysis of A549 cells using AK4 antibody (15-003). Blue: Western blot was performed from DAPI for nuclear staining. the immunoprecipitate using AK4 antibody (15-003) at a dilition of 1:1000.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human, Mouse
Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of IMMUNOGEN: human AK4 (NP_037542.1).
TESTED APPLICATIONS: IF, IP, WB
September 27, 2021 1 https://www.prosci-inc.com/ak4-antibody-15-003.html WB: ,1:500 - 1:2000
APPLICATIONS: IF: ,1:50 - 1:200
IP: ,1:50 - 1:200
POSITIVE CONTROL: 1) HepG2
2) Raji
3) HeLa
4) SW480
5) Mouse liver
6) Mouse kidney
PREDICTED MOLECULAR Observed: 25kDa WEIGHT:
Properties
PURIFICATION: Affinity purification
CLONALITY: Polyclonal
ISOTYPE: IgG
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.
Additional Info
OFFICIAL SYMBOL: AK4
ALTERNATE NAMES: AK3L1, AK3, AK4, AK 4, AK3L1, AK3L2
GENE ID: 205
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have BACKGROUND: been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1.
September 27, 2021 2 https://www.prosci-inc.com/ak4-antibody-15-003.html ANTIBODIES FOR RESEARCH USE ONLY.
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September 27, 2021 3 https://www.prosci-inc.com/ak4-antibody-15-003.html