Dr Rakesh Minocha USDA Forest Service Northeastern Research Station .. Box 640 (271 Mast Road) Durham, 03824

Telephone: 603-868-7622 Fax: 603-868-7604 -Mail: [email protected]

Research Publications:

01. Batra (Minocha) . 1976. Seasonal variations in Noranga Pond, Ambala City, Haryana. .Sc. (Hons) Thesis, Panjab University, Chandigarh. p.157. 02. Minocha R, Thompson HJ, Meeker LD and Herbst EJ. 1984. Isolation of rat mammary epithelial cells for polyamine analysis. Biochem. . 223:929-932. 03. Minocha SC, Minocha R and Kahl . 1984. Effects of 2,4- on polysomal profiles and poly(A)+ RNA in excised tissue of Jerusalem artichoke (Helianthus tuberosus .).. Physiol. Plant. 61:189-194. 04. Thompson HJ, Herbst EJ, Meeker LD, Minocha R, Ronan AM and Fite R. 1984. Effect of D,L-alpha-difluoromethylornithine in murine mammary carcinogenesis. Carcinogenesis 5:1649-1651. 05. Minocha R. 1985. Prevention of carcinogenesis and cancer cell proliferation by an inhibitor of polyamine biosynthesis. Ph.D. Dissertation, University of New Hampshire, Durham, NH, USA. p.166. 06. Thompson HJ, Meeker LD, Herbst EJ, Ronan AM and Minocha R. 1985. Effect of concentration of D,L-2-difluoromethylornithine on murine mammary carcinogenesis. Cancer Res. 45:1170-1173. 07. Minocha R and Haney JF. 1986. Temporal pattern of feeding response of Chaoborus larvae to starvation. J. Plankt. Res. 8:229-233. 08. Shortle WC and Minocha R. 1990. Applications of ion chromatography to study pollution effects in forest trees. Adv. Ion Chromatogr. 2: 299-306. 09. Minocha SC, Minocha R and Robie CA. 1990. High-performance liquid chromatographic method for the determination of dansyl-polyamines. J. Chromatogr. A. 511: 177-183 10. Minocha R, Minocha SC, Komamine A and Shortle WC. 1990. Role of polyamines in DNA synthesis and cell division in suspension cultures of Catharanthus roseus. In Flores HE, Arteca RN and Shannon JC (eds.), Polyamines and Ethylene: Biochemistry, Physiology, and Interactions, American Society of Plant Physiologists. p.343-345.

11. Minocha SC, Khan AJ, Papa and Minocha R. 1990. Polyamines and ethylene biosynthesis in relation to somatic embryogenesis in carrot (Daucus carota L.). In Flores HE, Arteca RN and Shannon JC (eds.), Polyamines and Ethylene: Biochemistry, Physiology, and Interactions, American Society of Plant Physiologists. p.339-342. 12. Minocha SC, Minocha R and Komamine A. 1991. The effects of polyamine biosynthesis inhibitors on -adenosylmethionine synthetase and S-adenosyl-methionine decarboxylase activities in carrot cell cultures. Plant Physiol. Biochem. 29: 231-237. 13. Minocha R, Minocha SC, Komamine A and Shortle WC. 1991. Regulation of DNA synthesis and cell division by polyamines in Catharanthus roseus suspension cultures. Plant Cell Rep. 10: 126-130. 14. Minocha R, Minocha SC, Long S and Shortle WC. 1992. Effects of aluminum on DNA synthesis, cellular polyamines, polyamine biosynthetic enzymes, and inorganic ions in cell suspension cultures of a woody plant, Catharanthus roseus. Physiol. Plant. 85: 417-424. 15. Minocha R and Shortle WC. 1993. Fast, safe, and reliable methods for extraction of major inorganic cations from small quantities of woody plant tissues. Can. J. For. Res. 23:1645-1654. 16. Minocha R, Kvaalen , Minocha SC and Long S. 1993. Polyamines in embryogenic cultures of Norway spruce (Picea abies) and red spruce (Picea rubens). Tree Physiol. 13: 365-377. 18. Sun D, Minocha R and Minocha SC. 1993. Genetic transformation of Populus to modulate polyamine metabolism. Proceedings of Fifth International Workshop on the IUFRO Working Party S2.04- 07 - Somatic Cell Genetics: Biotechnology of Trees held on October 18-22 at Balsain, Spain. p.215-220. 19. Minocha R and Shortle WC. 1994. Polyamines and inorganic ions extracted from woody tissues by freeze-thawing. In Vozzo JA (ed.), Research and Applications of Chemical Sciences in Forestry, Proceedings of the Southern Station Chemical Society Meeting held on February 1-2, 1994 at Starkville, MS. Gen. Tech. Rep. SO-104. New Orleans, Louisiana: U.S. Department of Agriculture, Forest Service, Southern Forest Experiment Station, p.28-37. 20. Minocha R, Shortle WC, Long S and Minocha SC. 1994. A rapid and reliable procedure for extraction of cellular polyamines and inorganic ions from plant tissues. J. Plant Growth Regul. 13: 187-193. 21. Minocha R, Shortle WC and Minocha SC. 1994. Somatic embryogenesis in Picea abies (Norway spruce) and Picea rubens (red spruce): Growth rates and polyamines (Abstr.). In Proceedings of Second International Symposium on the Applications of Biotechnology to Tree Culture, Protection, and Utilization held on October 2-6, 1994 at Bloomington, MN. Gen. Tech. Rep. NC-175, St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Forest Experiment Station: 20.

22. Minocha SC and Minocha R. 1994. HPLC method for polyamine analysis. LC.GC. 12:506. 23. Minocha R, Minocha SC and Simola . 1995. Somatic embryogenesis and polyamines in woody plants. In Jain SM, Gupta PK and Newton RJ (eds.), Somatic Embryogenesis in Woody Plants, Kluwer Academic Publishers, Dordrecht, The Netherlands. p.337-359. 24. Minocha SC and Minocha R. 1995. Historical aspects of somatic embryogenesis in woody plants. In Jain SM, Gupta PK and Newton RJ (eds.), Somatic Embryogenesis in Woody Plants, Kluwer Academic Publishers, Dordrecht, The Netherlands. p.9-22. 24. Minocha SC and Minocha R. 1995. Role of polyamines in somatic embryogenesis. In Somatic Embryogenesis and Synthetic Seed I. Vol. 30, Bajaj YPS (ed.), Springer-Verlag Publishers, London. p.53-70. 26. Zhou , Minocha R and Minocha SC. 1995. Physiological responses of suspension cultures of Catharanthus roseus to aluminum: Changes in polyamines and inorganic ions. J. Plant Physiol. 145: 277- 284. 27. Shortle WC, Smith KT, Minocha R and Alexeyev VA. 1995. Similar patterns of changes in stem wood calcium concentration in red spruce and Siberian fir. J. Biogeogr. 22:467-473. 28. Minocha R, Shortle WC, Lawrence GB, David MB and Minocha SC. 1996. Putrescine: A marker of stress in red spruce trees. In John H, Birdsey R and O’Brian (eds.), Proceedings of the Northern Global Change Program held on March 14-16, 1995 at Pittsburgh, PA, USDA Forest Service Gen. Tech. Rep. NE- 214. Radnor, PA. U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station. p.119-130. 29. Smith KT, Shortle WC, Minocha R and Alexeyev VA. 1996. Dynamics of calcium concentration in stemwood of red spruce and Siberian fir. In John H, Birdsey R and O’Brian K (eds.), Proceedings of the Northern Global Change Program held on March 14-16, 1995 at Pittsburgh, PA, USDA Forest Service Gen. Tech. Rep. NE-214. Radnor, PA. U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station. p.230-238. 30. Minocha R, Shortle WC, Coughlin DJ and Minocha SC. 1996. Effects of Al on growth, polyamine metabolism, and inorganic ions in suspension cultures of red spruce (Picea rubens). Can. J. For. Res. 26: 550-559. 31. Minocha R, Shortle WC, Lawrence GB, David MB and Minocha SC. 1997. Relationships among foliar chemistry, foliar polyamines, and soil chemistry in red spruce trees growing across the northeastern United States. Plant Soil. 191:109- 122. 32. Shortle WC, Smith KT, Minocha R, Lawrence GB and David MB. 1997. Acid deposition, cation mobilization, and biochemical indicators of stress in healthy red spruce trees. J. Environ. Qual. 26:871-876.

33. Shortle WC and Minocha R. 1999. Taxol: A Review. In Biotechnology and Plant Protection in Forestry Science, Raychaudhuri SP and Maramorosch K (eds.), Science Publishers, Inc. USA. p.1- 11. 34. Minocha R. 1999. Markers of environmental stress. Proceedings of an International Symposium on Sugar Maple Ecology and Health held on June 2-4, 1998 at Warren, PA. Gen. Tech. Rep. NE-261. PA: U.S. Department of Agriculture, Forest Service, NERS. p.106. 35. Minocha R, Smith DR, Reeves , Steele KD and Minocha SC. 1999. Polyamine levels during the development of zygotic and somatic embryos of Pinus radiata. Physiol. Plant. 105:155-164. 36. Minocha R, Long S, Maki H and Minocha SC. 1999. Assays for the activities of polyamine biosynthetic enzymes using intact tissues. Plant Physiol. Biochem. 37: 597-603. 37. Minocha SC and Minocha R. 1999. Genetic Transformation in Conifers In Somatic Embryogenesis in Woody Plants. Vol. 5, Jain SM and Gupta PK (eds.), Kluwer Academic Publishers, The Netherlands. p 291-312. 38. Shortle WC, Smith KT, Minocha R, Minocha SC, Wargo PM and Vogt K. 1999. Tree Health and Physiology in a Changing Environment. In Ecological Studies: Responses of Northern U.S. Forests to Environmental Change. Vol. 139, Mickler, RA, Birdsey RA and Hom J (eds.), Springer-Verlag. p.229-274. 39. Minocha R. and Jain SM. 2000. Tissue culture of woody plants and its relevance to molecular biology. In Molecular Biology of Woody Plants, Jain SM and Minocha SC (eds.), Kluwer Academic Publishers, The Netherlands. p.315-340. 40. Minocha R, Long S, Magill AH, Aber J and McDowell WH. 2000. Foliar free polyamine and inorganic ion content in relation to soil and soil solution chemistry in two fertilized forest stands at the Harvard Forest, Massachusetts. Plant Soil. 222:119-137. 41. Minocha R. 2000. Stress Test for Trees. Northern Woodlands Magazine. Crinth, VT, September Issue. p.25. 42. Bhatnagar P, Glasheen BM, Bains SK, Long SL, Minocha R, Walter C and Minocha SC. 2001. Transgenic manipulation of the metabolism of polyamines in Poplar (Populus nigra x maximoviczii) cells. Plant Physiol. 125: 2139-2153. 43. Minocha R, McQuattie C, Fagerberg , Long S and Woon Noh E. 2001. Effects of aluminum in red spruce (Picea rubens) cell cultures: Cell growth and viability, mitochondrial activity, ultrastructure and potential sites of intracellular aluminum accumulation. Physiol. Plant. 113:486-498. 44. Bhatnagar, P, Minocha R and Minocha SC. 2002. Genetic manipulation of the metabolism of polyamines in poplar cells. The regulation of putrescine catabolism. Plant Physiol. 128:1455-1469.

45. Wargo PM, Minocha R, Wong BL, Long RP, Horsley SB and Hall TJ. 2002. Measuring changes in stress and vitality indicators in limed sugar maple on the Allegheny Plateau in north-central Pennsylvania. Can. J. For. Res. 32: 629-641. 46. Quan , Minocha R and Minocha SC. 2002. Genetic manipulation of polyamine metabolism in poplar II. Effects on ethylene biosynthesis. Plant Physiol. Biochem. 40: 929-937. 47. Minocha R, Minocha SC and Long S. 2003. Monitoring environmental stress in forest trees using biochemical and physiological markers. In Sustainable Forestry, Wood Products, and Biotechnology. Espinel S, Barredo Y and Ritter E (eds.), DFA-AFA Press, Victoria-Gasteiz, Spain. p.109-116. 48. Minocha SC, Bhatnagar P, Minocha R and Long S. 2003. Genetic manipulation of polyamine metabolism in poplar. In Sustainable Forestry, Wood Products, and Biotechnology, Espinel S, Barredo Y and Ritter E (eds.), DFA-AFA Press, Victoria-Gasteiz, Spain. p.179-194. 49. Minocha R and Long S. 2004. Effects of aluminum on organic acid metabolism and secretion by red spruce cell suspension cultures and the reversal of Al effects on growth and polyamine metabolism by exogenous organic acids. Tree Physiol. 24:55-64. 50. Minocha R, Lee , Long S, Bhatnagar P and Minocha SC. 2004. Physiological responses of wild type and putrescine-overproducing transgenic cells of poplar to variations in the form and concentration of nitrogen in the medium. Tree Physiol. 24:551-560. 51. Bauer GA, Bazzaz FA, Minocha R, Long S, Magill A, Aber J and Berntson GM. 2004. Effects of chronic N additions on tissue chemistry, photosynthetic capacity, and carbon sequestration potential of a red pine (Pinus resinosa Ait.) stand in the NE United States. For. Ecol. Manage. 196:173-186. 52. Minocha R and Long S. 2004. Simultaneous separation and quantitation of amino acids and polyamines of forest tree tissues and cell cultures within a single high-performance liquid chromatography run using dansyl derivatization. J. Chromatogr. A. 1035 (1): 63- 73. 53. Minocha R, Minocha SC and Long S. 2004. Polyamines and their biosynthetic enzymes during somatic embryo development in red spruce (Picea rubens Sarg.). In vitro Cell. Dev. Biol. 40:572-580.