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Chemistry and Biochemistry 1 Chemistry and Biochemistry 1 students. Inquiries regarding these opportunities should be directed to CHEMISTRY AND the graduate program coordinator. BIOCHEMISTRY Degrees for the Department Bachelor Degree(s) Undergraduate Program Information Chemistry - Bachelor of Arts A degree in chemistry or biochemistry enables a student to pursue a wide variety of careers in: research, production, sales, management Biochemistry - Bachelor of Science and teaching. These degrees are also an excellent preparation for Chemistry - Bachelor of Science professional studies in medicine, dentistry, forensics, veterinary science, optometry, pharmacology, pharmacy and law. Master Degree(s) Chemistry majors who have completed the requirements for the Bachelor Chemistry - Master of Science of Science degree may receive American Chemical Society certification if they take one additional one-semester course which includes 1 credit of Doctoral Degree(s) laboratory. Chemistry - Doctor of Philosophy All departmental and nondepartmental requirements may not be taken S/ U and must earn a C- or better final grade. Minors for the Department Biochemistry - Undergraduate Minor This department does not have a foreign language requirement for any of its degrees. Chemistry - Undergraduate Minor Graduate Program Information Shelley Lusetti, Department Head The Department of Chemistry and Biochemistry offers programs leading Professors Arterburn, Herndon, Lyons, Rayson; Associate Professors to the MS and Ph.D. degrees in Chemistry in the areas of physical, Ashley, Houston, Lusetti, Maio, Yukl; Assistant Professors Baker, Carlisle, organic, inorganic, biological, and analytical chemistry. Admission Talipov, Windorff; College Associate Professor Dunlavy, College Assistant to these programs without deficiency is based on an undergraduate Professors Chinnasamy, Potenza; Emeritus Professors Eiceman, Gopalan, program essentially equivalent to that pursued by a chemistry or Johnson, Kuehn, Lara, Quintana, Smirnov biochemistry major at this university. All applying students must submit S. Lusetti, Department Head, Ph.D. (Wisconsin–Madison)– biochemistry; undergraduate transcripts, a personal statement and CV, and arrange for enzymology of DNA repair; J. B. Arterburn, Ph.D. (Arizona)– organic 3 letters of recommendation. All foreign students from undergraduate chemistry; synthetic medicinal and chemical biology; A. K. Ashley, Ph.D. programs taught in a language other than English must additionally (Colorado State)– biochemistry and toxicology; DNA replication and repair, submit TOEFL or IELTS scores and demonstrate adequate English cancer; C. A. Baker, Ph.D. (Florida State)– analytical chemistry; separation speaking and writing skills. science, micro- and nanotechnologies for neuroscience and astrobiology; The core course work required of students entering with no S. M. Carlisle, Ph.D. (Louisville)– biochemistry; bioinformatics, cancer previous graduate study in chemistry or biochemistry consists of and metabolic disease; J. W. Herndon, Ph.D. (Princeton)– organic chemistry; courses exploring the concepts of Energy, Structure, Dynamics, and organo-transition metal complexes, synthesis of biologically important cyclic Measurements as applied to all disciplines of chemistry and biochemistry compounds; K. D. Houston, Ph.D. (Texas- MD Anderson)– biochemistry; as well as short courses in Safety, Research Ethics, and Professional molecular mechanisms of hormone action in tumorigenesis; B. A. Lyons, Development. Successful completion of a Qualifying Exam taken after Ph.D. (Cornell)– physical biochemistry; NMR spectroscopic studies of the first year of coursework will determine whether a student is qualified signal transduction pathways in breast cancer; W. A. Maio, Ph.D. (Johns to pursue continued study at the M.S. or Ph.D. level. Ph.D. candidates Hopkins)– organic chemistry; total synthesis of marine natural products and must take at least 6 additional credits of specialized coursework chosen explorations of new chemical methods; G. D. Rayson, Ph.D. (Texas-Austin)– in consultation with the thesis committee while M.S. candidates must analytical chemistry, spectroscopy; M. R. Talipov, Ph.D. (Bashkir State)– take at least 3 additional credits. Ph.D. candidates must successfully Theoretical Physical Chemistry; electronic structure calculations, ab initio complete a Comprehensive Exam in order to be eligible to write and calculations, density functional theory calculations; C. J. Windorff, Ph.D. defend a Ph.D. thesis. All students are expected to participate in (UC-Irvine)- inorganic chemistry; organometallic f-element and transition discussion groups and department colloquia. metal chemistry, redox chemistry; E. T. Yukl, Ph.D. (Oregon Health and Science)– biochemistry; x-ray crystallography and spectroscopy of bacterial Since research is central in both the M.S. and Ph.D. programs, the early metalloproteins selection of a research advisor is encouraged. Students may choose to rotate through up to 3 research labs during their first semester Biochemistry Courses before selecting a research advisor. Financial support is provided to all BCHE 140. Introduction to Biochemistry graduate students during their first year through teaching assistantships. 1 Credit (1) Continued support may be provided through a research or teaching A description of the nature of inquiry in biochemistry, especially with assistantship, depending upon individual laboratory funding. All support respect to the interaction of chemistry and biology. Both historical is contingent upon satisfactory academic and research performance. In development and topics of current interest will be discussed. Graded S/U. addition, numerous traineeships and fellowships are available to qualified 2 Chemistry and Biochemistry BCHE 241. Introduction to Research in Biochemistry BCHE 424. Experimental Biochemistry I 1-3 Credits 3 Credits (1.25+6P) Techniques and procedures of biochemical research. May be repeated for Laboratory techniques required for experimentation with recombinant a maximum of 3 credits. DNA such as nucleic acid isolation and purification, polymerase chain Prerequisites: 8 credits of chemistry and 3.0 GPA in chemistry. reaction (PCR), sequence analysis, and directed mutagenesis using genetic material from both prokaryotic and eukaryotic organisms. BCHE 341. Survey of Biochemistry 4 Credits (3+3P) Prerequisite(s): C- or better in BCHE 395, and BCHE 396 or GENE 315. Basic principles of biochemical processes and the structure/function Learning Outcomes 1. Conduct experiments safely of the major classes of biomolecules, with introductions to metabolism and the central dogma of biochemistry. The chemical and biological 2. Select and manipulate plasmids to achieve desired recombinant DNA properties of major biomolecules (DNA, proteins, May be repeated up to 4 for experimentation credits. 3. Obtain relevant DNA sequence information for gene of interest from Prerequisite(s): C- or better in CHEM 2115 or CHEM 314. public databases BCHE 395. Biochemistry I 4. Make buffers and reagents necessary for transforming and isolating 3 Credits (3) plasmid DNA from E. coli Principles governing chemistry and physics of life processes with 5. Transform and isolate plasmid DNA to be used for cloning of gene of emphasis on the relationships between molecular structure and cell interest function. Basic principles of biochemical processes, enzymology, 6. Design primers for PCR to enrich gene of interest from genomic DNA and the structure/function of the major classes of biomolecules with 7. Analyze DNA sequence and choose appropriate restriction enzymes introductions to metabolism. Introduction to catabolic metabolism. for cloning gene of interest Prerequisite(s): C or better in CHEM 314. 8. Design primers for PCR to incorporate restriction sites at the ends of BCHE 396. Biochemistry II gene of interest 3 Credits (3) 9. Perform restriction digest on plasmid DNA and PCR products Introduction to anabolic metabolism and hormonal regulation. 10. 1 Perform ligation reaction to combine gene of interest with plasmid Biochemical principles of the mechanism and regulation of replication, DNA transcription, recombination and translation in prokaryotes and eukaryotes. Introduction to DNA-based information technology. Taught 11. 1 Analyze sequence of plasmid containing gene of interest to validate with BCHE 396 H. the outcome of experimentation Prerequisite(s): C or better in BCHE 395. BCHE 425. Experimental Biochemistry II BCHE 396 H. Biochemistry II Honors 3 Credits (3) 3 Credits (3) Introduction to fundamental techniques used to explore structure Taught with BCHE 396 with additional work required. and function of biological macromolecules such as proteins, carbohydrates, lipids, and nucleic acid. Course covers analyzing and reporting experimental data; enzymology; quantitative methods to determine biological molecules; basic principles of electrophoresis, chromatography, and spectroscopic immunochemistry. May be repeated up to 3 credits. Prerequisite(s): C- or better in BCHE 424. Learning Outcomes 1. Understand and implement various methods of protein purification as well as qualitative and quantitative analysis of protein preparations 2. Become proficient in absorbance and fluorescence spectroscopy 3. Determine ligand binding parameters 4. Understand and measure enzyme kinetics and inhibition 5. Perform basic protein crystallization and structure determination 6. Develop skills in scientific writing and presentation Chemistry and Biochemistry 3 BCHE 432. Physical
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