21.10.2014STAU1 antibody (pAb)

Rabbit Anti -Human/Mouse/Rat RNA -binding Staufen homolog 1

Instruction Manual

Catalog Number PK-AB718-5997 Synonyms STAU1 Antibody: RNA-binding protein Staufen homolog 1, STAU Description STAU1 is a member of a family of double-stranded RNA (dsRNA)-binding involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind having double-stranded secondary structures. The human STAU1 also contains a - binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. STAU1 has been shown to be present in the cytoplasm in association with the rough (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER. STAU1 is also known to interact with influenza ribonucleoproteins NS1, NP, and PA and is required for efficient viral replication. Quantity 100 µg Source / Host Rabbit Immunogen STAU1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human STAU1. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse, Rat Specificity Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB, IHC, IF |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. STAU1 antibody can be used for detection of STAU1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL. Images Available upon request. References Marion RM, Fortes P, Peloso A, et al. A human sequence homologue of Staufen is an RNA-binding protein that is associated with polysomes and localizes to the rough endoplasmic reticulum. Mol. Cell. Biol.1999; 19:2212-9. de Lucas S, Peredo J, Marion RM, et al. Human Staufen1 protein interacts with influenza virus nucleoproteins and is required for efficient virus multiplication. J. Virol.2010; 84:7603-12.

Images Available upon request. Related Products Cat. No. PK-AB718-5997P - STAU1 Peptide Cat. No. PK-AB718-1463 - Rat Brain Tissue Lysate

FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014