FOODQS GMBH Bernd Kämpf

The laboratory for Food Analytics and Quality control FoodQS GmbH ➢ Commercial lab in Germany

➢ More than 20 years experience in the anlytics of beeproducts worldwide

➢ Further analytics ❖ beeswax ❖ Bee pollen Langenzenn (Nuremberg) ❖ Royal jelly ❖ Syrups e.g. Agave syrup, maple syrup…

➢ Accreditation to DIN EN ISO 17025 Honey analytics

Pyrrolizidin Chem.phys. Antibiotics parameters -alcaloides NMR Marker sugars

honey Heavy Pollen foreign Isotopics analysis metals enzymes

veterinary Oligo- GMO PCB Pesticides drugs saccharide Authenticity: Analytics of adulterations

Pollen Enzymes Sugars Markers Isotopics NMR

Rice syrup Delta β-Amylase dextrose Botanical origin marker 13C/12C (RSM) foreign γ-Amylase oligo- Geographical saccharides Arsenic origin Honey foreign E150 α-Amylase

β- Sugar beet Fructofura- marker nosidase (SMB)

Psicose LC-HRMS Our approach using LC-HRMS since 2016

- Just for research and development

- Searching for marker substances in various syrups

- Identification of this marker substances to her correct chemical name

- Proof and checking if the substance is only available in syrups and not naturally occuring in any type of honey (honey database necessary)

- Explanation: Why is the marker substance in this syrup (way of production). Check to plausibility

- Transfer the method/ way of detection to the more sensitive LC-MS/MS (if possible) summary

- Identification of SMB as 3-Methoxytyramin (published in July 2019 in cooperation with QSI)

- Identification of RSM as Glycosylisomaltol

- Identification of further markers (published in June 2019)

Product based on markers

▪ 11 marker substances in one order to get the best information regarding honey adulteration (sensitive LC-MS/MS analytics) ▪ different syrups were considered depending on the marker (beet sugar, rice, wheat,…)

Additional if needed: NMR analytics and isotopic analytics The End

Thank you for your attention!