Alexandre Jousset

Georg-August University Göttingen, Animal Ecology Berlinerstr. 28, 37073 Göttingen, Germany E-mail : [email protected] Phone (prof.): +49551-39.5468 Phone (private): +49176-631.61.624

Personal data

Birth Date: April 27th, 1981 in Lausanne, Switzerland Nationality: French, Swiss Sex: Male

Languages

French: Mother Tongue German: Fluent (written and spoken) Spanish: Fluent (written and spoken) English: Fluent (written and spoken) Chinese: basic knowledge

Research interests I investigate the ecology and evolution of fluorescent pseudomonads involved in plant protection against pathogens. I use these bacteria as model system to understand how biotic interaction affecting the performance and evolution of living communities. In addition, I apply biotic interactions to improve strategies of biological control of plant pathogens.

Keywords: Microbial ecology, biodiversity, crop protection, predator-prey interactions, microevolution

Professional Experience

2009-present Lecturer („Wissenschaftlicher Mitarbeiter“), Georg-August University Göttingen.

2006-2009 PhD, Technische Universität Darmstadt, Germany: “Importance of biotic interactions for the fitness and activity of rhizosphere biocontrol pseudomonads”

2005 Reserch stay, Universidad Nacional de Quilmes, Argentina: Interactions between biocontrol bacteria Pseudomonas fluorescens CHA0 and protozoan predators (Jousset et al 2006)

2004 Umweltforschungszentrum (UFZ), Leipzig, Germany: Investigation of the effect of pesticides on soil protozoa

Education

2003-2004 Diploma in Biology (MSc equivalent) Swiss Federal Institute of Technology, Lausanne, (Supervision: Antonis Chatzinotas & Hauke Harms)

Publications 1. Eisenhauer, N., Schulz, W., Scheu, S., Jousset, A., (2012): Niche dimensionality links biodiversity and invasibility in microbial communities. Functional Ecology in press. 2. Becker, J., Eisenhauer, N., Scheu, S., and Jousset, A. (2012) Increasing antagonistic interactions cause bacterial communities to collapse at high diversity. Ecology Letters 15: 468-474. 3. Eisenhauer, N., Scheu, S., and Jousset, A. (2012) Bacterial diversity stabilizes community productivity. PLoS ONE 7: e34517. 4. Jousset, A. (2012) Ecological and evolutive implications of bacterial defences against predators. Environmental Microbiology 14:1830-1843.(Invited review) 5. Latz, E., Eisenhauer, N., Rall, B.C., Allan, E., Roscher, C., Scheu, S., and Jousset, A. (2012) Plant diversity improves protection against soil-borne pathogens by fostering antagonistic bacterial communities. Journal of Ecology 100: 597-604.(Notebook article in “The Scientist”) 6. Henkes, G., Jousset, A., Bonkowski, M., Thorpe, M.R., Scheu, S., Schurr, U., and Röse, U.S.R. (2011) Pseudomonas fluorescens CHA0 maintains carbon delivery to Fusarium graminearum infected roots and prevents reduction in biomass of barley shoots through systemic interactions. Journal of Experimental Botany 62: 4337-4344. 7. Jousset, A., Rochat, L., Lanoue, A., Keel, C., Bonkowski, M., and Scheu, S. (2011) Plants respond to pathogen infection by enhancing the antifungal activity of root-associated bacteria. Molecular Plant-Microbe Interactions 24: 352-358. 8. Jousset, A., Schmid, B., Scheu, S., and Eisenhauer, N. (2011) Genotypic richness and dissimilarity opposingly affect ecosystem performance. Ecology Letters 14: 537-624 (cited in various German newspapers including die Welt, Göttinger Tagesblatt, Berliner Morgenpost) 9. Jousset, A., Schulz, W., Scheu, S., and Eisenhauer, N. (2011) Intraspecific genetic richness and relatedness predict the invasibility of bacterial communities ISME Journal 5: 1108-1114. (Research Highlight in Nature Reviews Microbiology 9:312-313) 10. Neidig, N., Paul, R., Scheu, S., and Jousset, A. (2011) Secondary metabolites of Pseudomonas fluorescens CHA0 drive complex non-trophic interactions with bacterivorous nematodes. Microbial Ecology 61: 853-859. 11. Jousset, A., and Bonkowski, M. (2010) The model predator Acanthamoeba castellanii induces the production of 2,4- DAPG by the biocontrol strain Pseudomonas fluorescens Q2- 87. Soil Biology & Biochemistry 42: 1647-1649. 12. Jousset, A., Rochat, L., Scheu, S., Bonkowski, M., and Keel, C. (2010) Predator-prey chemical warfare determines the expression of antifungal genes by rhizosphere pseudomonads. Applied and Environmental Microbiology 76: 5263-5268. (rated “9 ” by Faculty of 1000) 13. Neidig, N., Jousset, A., Nunes, F., Bonkowski, M., Paul, R.J., and Scheu, S. (2010) Interference between bacterial feeding nematodes and amoebae relies on innate and inducible mutual toxicity Functional Ecology 24: 1133-1138. 14. Jousset, A., Lara, E., Nikolausz, M., Harms, H., and Chatzinotas, A. (2009) Application of the denaturing gradient gel electrophoresis (DGGE) technique as an efficient diagnostic tool for ciliate communities in soil. Sci Total Environ 408: 1221-1225. 15. Jousset, A., Rochat, L., Keel, C., Pechy-Tarr, M., Scheu, S., and Bonkowski, M. (2009) Predators promote toxicity of rhizosphere bacterial communities by selective feeding on non-toxic cheaters. ISME Journal 3: 666-674.(Research Highlight in Nature Reviews Microbiology 7:326- 327) 16. Schebb, N.H., Vielhaber, T., Jousset, A., and Karst, U. (2009) Development of a liquid chromatography-based screening methodology for proteolytic enzyme activity. Journal of Chromatography a 1216: 4407-4415. 17. Jousset, A., Scheu, S., and Bonkowski, M. (2008) Secondary metabolite production facilitates establishment of rhizobacteria by reducing both protozoan predation and the competitive effects of indigenous bacteria. Functional Ecology 22: 714-719. 18. Jousset, A., Lara, E., Wall, L.G., and Valverde, C. (2006) Secondary metabolites help biocontrol strain Pseudomonas fluorescens CHA0 to escape protozoan grazing. Applied and Environmental Microbiology 72: 7083-7090.

Contributions to meetings (selected) Jousset A, Merker M, Eisenhauer N, Scheu S, 2011.” High genetic diversity accelerates adaptive radiation in microbial microcosms", XIII congress of the European Society for Evolutionary Biology (ESEB), 20-25 August, Tübingen, Germany

Jousset A, Becker J, Scheu S, Eisenhauer N, 2010. “High intraspecific diversity results in collapse of plant associated Pseudomonas fluorescens communities” (Poster), 13rd International Symposium on Microbial Ecology (ISME), 22-27 August 2010, Seattle, USA.

Jousset A, Kalinkat G, Brose U, Scheu S, Rall B, 2010. “Predation resistant bacteria create a protective niche for non-toxic cheaters” (Oral presentation). 3rd joint conference DGHM-VAAM, 28-31 March 2010, Hannover, Germany.

Jousset A, Rochat L, Keel C, Scheu S, Bonkowski M, 2009 “Predator-prey chemical warfare determines the production of antifungal compounds by a root-associated pseudomonad” (Oral presentation). 8th PGPR Workshop, 17-22 May 2009, Portland, USA.

Jousset A, Rochat L, Pechy-Tarr M , Keel C, Scheu S, Bonkowski M, 2008. “Predators promote cooperative defense and repress cheating by rhizosphere bacteria” (Oral presentation). 12th International Symposium on Microbial Ecology (ISME), 17-22 August 2008, Cairns, Australia.

Invited talks (selected) 2012 “Harnessing the power of biodiversity”, JKI Institute Braunschweig, Germany “Diversity may harm you and the competitors around you”, CNRS Montpellier, France. 2011 “Impact of Biodiversity for the functioning of microbial communities”, Nanjing University, China “Allelopathy, biodiversity and complementarity: exploring the ecological ABC with model bacterial communities”. University of Minnesota, St- Paul, USA. 2010 “Model organisms in soil ecology”, Workshop of the German society for Ecology 2008 “Love-hate relationship between biocontrol bacteria and their predators”, University of Groningen, Netherlands

Teaching Since 2009 Practical work and seminar “functional soil ecology” (120 h/semester). University of Göttingen 2008 Practical work and seminar “soil ecology” (40 hours). TU Darmstadt

Supervised students

Nine bachelor theses, eight diploma/master Theses, two PhD supervised since 2007 Bachelor theses (12 weeks) 2012 Anna Hille. Effect of nutrient availability and diffusion rate on the fitness of social cheaters in Pseudomonas fluorescens. 2011 Leonard Drees. Importance of temperature and nutrient availability on the expression of biocontrol genes by Pseudomonas fluorescens CHA0 Lennart Holst. Effect of commensal yeasts on the resistence of Drosophila melanogaster to killing by Pseudomonas fluorescens. Monika Merker. Importance of genetic recombination for bacterial fitness in diverse communities. Mona Schlottmann. Chemical communication between Pseudomonas fluorescens and Fusarium graminearum. 2010 Eva Materne. Effect of intraspecific diversity on the success of cheating Pseudomonas fluorescens.

Wiebke Schulz. Effect of biodiversity and resource complexity on the invasibility of bacterial communities. 2008 Ellen Latz. Dispersion of Pseudomonas fluorescens CHA0 by nematodes, collemboles and earthworms. 2007 Nina Neidig. Mutual inhibition of the soil amoeba Acanthamoeba castellanii and the nematode Caenorhabditis elegans. (Neidig et al., Functional Ecology 2010) Diploma /Master theses (6 months) 2012 Paul Götsch. Effect of species richness on the performance of PAH degrading microorganism communities Simone Weidner. Effect of intraspecific diversity of rhizosphere bacteria on the N uptake of Arabidopsis thaliana. Katharina Fußmann. The paradox of warming: Warming dampens predator-prey oscillation (co-supervision with B. Rall) 2011 Maren Stella-Müller,. Effect of the model predator Acanthamoeba castellanii on bacterial communities responsible for soil suppressiveness against the pathogen Rhizoctonia solani.(Stella-Müller et al., in revision) 2010 Joachim Becker. Impact of intraspecific diversity on the growth and plant protective potential of biocontrol Pseudomonas fluorescens communities. (Becker et al. Ecology Letters, 2012) Jan Hendrik Dudenhöffer. Recruitment of protective bacterial communities by barley plants as response to infection with Fusarium graminearum. 2009 Nina Neidig. Impact of the exotoxins from the biocontrol bacterium Pseudomonas fluorescens CHA0 on the behaviour, stress response (DAF pathway) and survival of Caenorhabditis elegans. (Neidig et al., Microbial Ecology 2011) Ellen Latz. Effect of plant diversity on soil suppressiveness and bacterial communities with biocontrol potential. (Latz et al., J Ecology 2012) PhD Thesis Since 2011 Ellen Latz. The role of plant diversity for the promotion of bacterial communities responsible for soil suppressiveness against phytopathogens Since 2012 Simone Weidner. Impact of soil microbial diversity on plant-microbe interactions

Funding

2011-2014 DFG research grant “The role of plant diversity for the promotion of bacterial communities responsible for soil suppressiveness against phytopathogens” (DFG, JO 935/2, 170‟000€) 2011-2012 Cooperation project with the University of Nanjing, China. « Diversitätsansatz zur Verbesserung bestehender Bioremediationsstrategien für PAK kontaminierte Böden » (BMB-F, CHN 11/031, 15„000 €, submitted together with Dr. Butenschön). 2010 Travel stipend for visiting the ISME XIII Meeting (DAAD, 2000 €) 2006-2009 PhD Fellowship from the Deutsche Bundesstiftung Umwelt (DBU, 40‟000 €)

Further qualifications 2011-12 Certificate “didactic for university teaching” (Hochschuldidaktik), 150 hours qualification program. 2011 Qualified to work with pathogenic microorganisms (§44 Infektionsschutzgesetz) 2010 Supervision of the renovation and declaration of laboratories as security class S2 (opportunistic pathogens) for genetic studies.

2008 Certificate “Sicherheit in der Gentechnik” (University of Mainz).

Others Organisation of the 2011 soil ecology workshop of the German Society for Ecology Organisation of the 2011 and 2012 GoEvol meeting for evolutionary biology, Göttingen

Reviewer for the following journals: ISME Journal, Environmental Microbiology, New Phytologist, PLoS ONE, Applied and Environmental Microbiology, Soil Biology & Biochemistry, Journal of Experimental Botany, FEMS Microbiology Ecology, Microbial Ecology, European Journal of Soil Science, Canadian Journal of Microbiology, Pedobiology, Applied Soil Ecology, Plant & Soil, Canadian Journal of Microbiology, Biology & Fertility of Soils, Current Microbiology, Acta Protozoologica.

Collaborations Nico Eisenhauer (Schiller University Jena, Germany): Interactions between diverse grassland communities and soil microbes Ville Friman (University of Exeter, UK): Molecular mechanisms underlying bacteria-phage coevolution Rong Ji (Nanjing University, China): Bacterial diversity and the biodegradation of organic pollutants Sonia Kefi & Nicolas Mouquet (CNRS Montpellier, France): Modeling of the effect of antagonistic interactions on the biodiversity-ecosystem functioning relationship in microbial microcosms Katrine Lapouge (University of Lausnane, Switzerland): Environmental factors affecting the regulation of secondary metabolism in Pseudomonas fluorescens. Tillmann Lüders (Helmholtz center Munich): RNA-based Stable Isotope Probing Monika Maurhofer (ETHZ, Switzerlan), Christoph Keel (University of Lausanne, Switzerland), Andrea Thürmer (University of Göttingen): Sequencing and annotation of the genome of Pseudomonas fluorescens CHA0. Björn Rall (Universität Göttingen, Germany): Effect of temperature on predator-prey dynamics I in microbial microcosms Liliane Rueß (HU Berlin, Germany): Functional response of bacterivorous nematodes Claudio Valverde (UNQ, Argentina): Beneficial microbial communities in agricultural soils