Mouse Anti-Cathepsin V Catalog No

Qty: 100 μg/200 µL Mouse anti-Cathepsin V Catalog No. 414900 Lot No. Mouse Anti-Cathepsin V

FORM This affinity-purified mouse monoclonal antibody is supplied as a 200 µL aliquot at a concentration of 0.5 mg/mL in PBS, pH 7.4, containing 0.1% sodium azide. This antibody is highly purified from mouse ascites by protein A chromatography.

Clone: 3G11 Isotype: IgG1

IMMUNOGEN Peptide corresponding to amino acid residues from the C-terminal region of human cathepsin V protein (accession # O60911, NP_001324), which is 99% homologous with chimpanzee, 94% rhesus monkey, 82% canine, and 79% with bovine.

SPECIFICITY This antibody is specific for the cathepsin V (cathepsin L2, cathepsin U) protein. On Western Blots of human recombinant pro-cathepsin V, it identifies the target band at ~40 kDa. This antibody also can identify a 24 kDa mature two-chain cathepsin V. This antibody does not cross react with closely related human cathepsin L which has 78% sequence identity, and is negative to human cathepsins B, F, H, K, S, and W.

REACTIVITY Reactivity has been confirmed with recombinant human pro-cathepsin V protein by Western Blotting, and with normal human keratinocytes by immunofluorescence. Based on amino acid sequence homology, reactivity with chimpanzee, rhesus monkey, canine and bovine is also expected.

Sample Western Immuno- Blotting fluorescence Human +++ +++ Chimpanzee ND ND Rhesus monkey ND ND Canine ND ND Bovine ND ND (Excellent +++, Good ++, Poor +, No reactivity 0, Not applicable N/A, Not determined ND)

USAGE Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Western Blotting: 2 µg/mL Immunofluorescence: 2.5-5 µg/mL

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www.invitrogen.com Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288 • E-mail: [email protected] PI414900 (Rev 10/08) DCC-08-1089 Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website, www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

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STORAGE Store at 2-8°C for up to one month. Store at –20°C for long-term storage. Avoid repeated freezing and thawing.

BACKGROUND Cathepsins are a large family of lysosomal cysteine proteases that have a vital role in mammalian cellular turnover, e.g. bone resorption. They degrade polypeptides and are distinguished by their substrate specificities. There are approximately a dozen members of this family, which are distinguished by their structure and which proteins they cleave. Most of the members become activated at the low pH found in lysosomes. Thus, the activity of this family lies almost entirely within those organelles.1 Cathepsins can be used as biological markers of malignant tumors and have been associated with Alzheimer and stroke. They can be used as targets for cancer therapy and prognosis of cancer.2

Degradation of the arterial elastin matrix contributes to the loss of elasticity of the arterial wall resulting in atherosclerosis. A novel human elastolytic cysteine protease, cathepsin V, also known as cathepsin L2 and cathepsin U has been detected in atherosclerotic plaque specimens. Cathepsin V is expressed by macrophages and exhibits the most potent elastase activity yet described among human proteases.3 Cathepsin V, specifically expressed in the thymus, testis, and corneal epithelium, can be autocatalytically converted to the mature form at pH 4.0.

REFERENCES 1. Bromme D, et al. Biochemistry 38(8):2377-85, 1999. 2. Nomura T, et al. J Med Invest 52(1-2):1-9. Review, 2005. 3. Yasuda Y, et al. J Biol Chem 79(35):36761-70, 2004.

RELATED PRODUCTS Product Conjugate Cat. No. Protein A Sepharose 4B 10-1041 rec-Protein G Sepharose 4B 10-1241 ZyMAX™ Goat anti-rabbit IgG Unconjugated 81-6100 ZyMAX™ Goat anti-mouse IgG Unconjugated 81-6500

Secondary antibody conjugates. Goat anti-rabbit IgG Goat anti-mouse IgG Fluorescence Conjugate Ex/Em* (H+L) (H+L) similar to-- Alexa Fluor® 488 A11008 A11001 495/519 FITC Alexa Fluor® 555 A21428 A21422 555/565 Cy3 Alexa Fluor® 594 A11012 A11005 590/617 Texas Red Alexa Fluor® 647 A21244 A21235 650/668 Cy5 HRP 81-6120 81-6520 NA** NA AP 81-6122 81-6522 NA NA Biotin B2770 B2763 NA NA *Excitation/emission (nm); **Not applicable

For additional secondary antibody conjugates, visit www.invitrogen.com/antibodies

For Research Use Only