CURRICULUM VITAE

DATE: March 15, 2019

NAME: Davide Comoletti, DVM, Ph.D.

PRESENT TITLE: Note that Dr. Comoletti has a dual faculty appointment Senior Lecturer School of Biological Sciences Victoria University of Wellington, New Zealand

and

Adjunct Professor Departments of Neuroscience and Cell Biology and Dept. of Pediatrics Child Health Institute of New Jersey Rutgers - Rutgers-Robert Wood Johnson Medical School

HOME ADDRESS: 11 Malda Grove Khandallah, Wellington, 6035 New Zealand

OFFICE ADDRESS: School of Biological Sciences Victoria University of Wellington PO Box 600 Wellington 6140, NZ Alan MacDiarmid (AMD) Building

and

Child Health Institute of New Jersey 89 French Street New Brunswick, NJ, 08901 Floor 3 Room 3275

TELEPHONE NUMBER/E-MAIL ADDRESS: Office: +64-04-463-6029 Fax: Web: http://rwjms1.umdnj.edu/comoletti_lab/

CITIZENSHIP: Italian, American (2016)

EDUCATION: A. Undergraduate and Professional Agricultural Technician. Istituto Tecnico Agrario Statale “Italo Calvino” 07/1984 Noverasco di Opera, Milan, Italy

B. Graduate and Professional Doctor Veterinary Medicine, Veterinary School University of Milan, Milan, Italy 03/1995

Graduate Student, Ph.D, degree 07/1998 “Mario Negri” Institute for Pharmacological Research Laboratory of Receptor Pharmacology (P.I.: Dr. Tiziana Mennini) Milan, Italy

POSTGRADUATE TRAINING:

A. Postdoctoral Appointments Page 1 of 9

Department of Pharmacology University of California, San Diego (School of Medicine) Postgraduate Researcher 08/1998-05/2004

MILITARY: Mandatory service for the Italian Army, 5/1992-4/1993

ACADEMIC APPOINTMENTS: 2018 – Present: Senior Lecturer School of Biological Sciences Victoria University of Wellington

2011 – 2018: Assistant Professor, Tenure track Department of Neuroscience and Cell Biology and Pediatrics Child Health Institute of New Jersey Rutgers-Robert Wood Johnson Medical School 01/2007-08/2011: Assistant Research Scientist University of California, San Diego (School of Pharmacy) Skaggs School of Pharmacy & Pharmaceutical Sciences

06/2004-12/2006: Assistant Project Scientist Department of Pharmacology University of California, San Diego (School of Medicine)

MEMBERSHIPS, OFFICES AND COMMITTEE ASSIGNMENTS IN PROFESSIONAL SOCIETIES: 2003-2005 Protein Society 2005 – Present Society for Neuroscience 2017 – Present Senior member of the Rutgers Brain Health Institute (BHI)

HONORS AND AWARDS: 1998-1999 Graduate fellowship, “Mario Negri” Institute for Pharmacological Research

SERVICE ON NATIONAL GRANT REVIEW PANELS, STUDY SECTIONS, COMMITTEES: 2018: reviewer for the Multiple Sclerosis Italian Foundation (FISM). 05/2017: Reviewer for The Leverhulme Trust (UK) grant proposal 02/2016 – Member of the NIH Biophysics of Neural Systems Study Section [BPNS] 09/2015 – Reviewer for National Science Foundation, Division of Integrative Organismal Systems Core Programs 01/2014 – Present: Reviewer for the Italian Ministry of Health, General Direction for Scientific Research and Health Innovation. 04/2014 – Reviewer for Medical Research Council UK. 03/2014 – Reviewer for Research Foundation - Flanders (Fonds Wetenschappelijk Onderzoek - Vlaanderen, FWO) 05/2012 - Reviewer for Neurological Foundation of New Zealand 2006 - 2012: Reviewer for Autism Speaks Foundation

SERVICE ON MAJOR COMMITTEES: A. International Organizer (Chair) of the “The Neuronal Surfaceome in Circuit Formation: From Structure to Function” meeting (July 10-11, 2017) in Anzola D’Ossola, Italy. B. National Member of the Scientific Committee for the organization of the 20th International Analytical Ultracentrifugation Conference 2012 (http://auc2012.uthscsa.edu/index.php) C. Medical School/University N/A D. Hospital N/A E. Department 2012: Organizer of the Faculty Science Talks among resident faculties of CHINJ 2012 - Present: Co-founder with Drs. Zhiping Pang of NeuroConnections Club (NCC), a lecture series for graduate students and postdocs to showcase their work in progress within the Neuroscience Program. 2012 - 2013: co-organizer with Drs. Pang, Mladen-Roko Rasin, and Huaye Zhang of the extramural speaker talks. 2012 – Present: member of the selection committee of the Neuroscience program for prospective graduate students.

Page 2 of 9

2012 – Present: member of the selection committee for Faculty recruitment within the Graduate Programs in Molecular Biosciences 2017: Search Committee member for the Herbert C. and Jacqueline Krieger Klein Endowed Chair in Alzheimer’s Disease and Neurodegeneration. 2017: Summer Fellowship Review Committee, M1&M2 students, RWJMS. Director: Celine Gelinas, Associate Dean for Research F. Editorial Boards 07/2016 – Present: Editor for the Journal of Biological Chemistry (JBC) 09/2015 – Present: Review Editor in Frontiers in Molecular Neuroscience G. AdHoc Reviewer for the following Scientific Publications: 2010 - Present: Scientific publications: Journal of Neuroscience Methods; Human Molecular Genetics; Neurobiology of Disease; Biochimica et Biophysica Acta - Molecular Cell Research; Journal of Neurochemistry, Journal of Biological Chemistry, Science Signaling, Journal of Neuroscience, Biophysical Journal, PeerJ, Nature Communications

SERVICE ON GRADUATE SCHOOL COMMITTEES: 2013 - Member of the MBS qualifying exam committee (Chair, Dr. Kiran Madura) 2014 - Member of the MBS qualifying exam committee (Chair, Dr. William G. Wadsworth) 2017 – PhD Committee member of Emily Kelly-Castro (P.I: Huaye Zhang) 2017 – PhD Committee member of Laura Trobiani (P.I: Antonella De Jaco, Rome, Italy)

SERVICE ON UNDERGRADUATE SCHOOL COMMITTEES: 2012 – 2013: Aresty Program Mentor for Irene Pakos 2012 – 2013: Aresty Program Mentor for Zinal Chhangawala 2015 – 2016: Aresty Program Mentor for Sumie Kakehi 2016 – 2017: Aresty Program Mentor for Khush Patel 2015 - 2018: Member of the Aresty Faculty Review Board

SPONSORSHIP OF CANDIDATES FOR POSTGRADUATE DEGREE: 2017 – Present: Liam Turk 2014 – 2017: Vincent Mirabella 2012 –2016: Eva N. Rubio-Marrero

SPONSORSHIP OF POSTDOCTORAL FELLOWS: 2012 - 2013: Dr. Hyuck Kim 2013 – 2018: Dr. Fanomezana, Ranaivoson 2014 – 2018: Dr. Sinem Ozgul

TEACHING RESPONSIBILITIES: A. Lectures or Course Directorships Discussion leader: February 2009: University of California, San Diego, Department of Pharmacology, Discussion leader in “Integration of Signaling Pathways”, Two-hour class, two classes taught. October 2011: Discussion leader in “Molecular Medicine” course. The Cancer Institute of New Jersey 2011. The class is coordinated by Dr. Sunita Chaudhary. September 2012 – April 2013 – M1 Histology laboratories, nine labs, 2hrs each. October 10th, 2012: Topics: Mol Cell Biol "Survival skills”. Protein Structure Analysis/Comparison Tools class. Coordinators: Nancy Walworth and Rick Padgett, Co-Directors, Joint Graduate Programs in Molecular Biosciences September 2013 – January 2014 – M1 Histology laboratories, nine labs, 2hrs each. September 2014 – January 2015 – M1 Histology laboratories, nine labs, 2hrs each. March 26th 2014: Topics: Mol Cell Biol "Survival skills”. Protein Structure Analysis/Comparison Tools class. Coordinators: Nancy Walworth and Rick Padgett, Co-Directors, Joint Graduate Programs in Molecular Biosciences April 15th 2015: Topics: Mol Cell Biol "Survival skills”. Protein Structure Analysis/Comparison Tools class. Coordinators: Nancy Walworth and Rick Padgett, Co-Directors, Joint Graduate Programs in Molecular Biosciences September 2015 – January 2016 – M1 Histology laboratories, nine labs, 2hrs each. March 30, 2016: Topics: Mol Cell Biol "Survival skills”. Protein Structure Analysis/Comparison Tools class. Coordinators: Nancy Walworth and Rick Padgett, Co-Directors, Joint Graduate Programs in Molecular Biosciences February 9th, 2016 - Biological, Biomedical and Social Aspects of Aging. Course Director, Dr. Federico Sesti. Rutgers 16:761:610 spring 2016 Page 3 of 9

March-April 2016 - Neuroscience Program, Advanced studies, 16:710:606:01, spring 2016 Three 2-hr class, Program Director, Dr. John Pintar. September 2016 – January 2017 – M1 Histology laboratories, nine labs, 2hrs each January 31st, 2017 – Lecture: Aging and caloric restriction. Course: Biological, Biomedical and Social Aspects of Aging. Course Director, Dr. Federico Sesti. Rutgers 16:761:610 spring 2017 March 2, 2017: lecture: Brain Adhesion Molecules in Plasticity and Disease. Course: Neural Injury And Repair 16:963:632 March 29, 2017: Topics: Mol Cell Biol "Survival skills”. Protein Structure Analysis/Comparison Tools class. Coordinators: Nancy Walworth and Rick Padgett, Co-Directors, Joint Graduate Programs in Molecular Biosciences September 2017 – January 2018 – M1 Histology laboratories, nine labs, 2hrs each January 23, 2018 – Lecture: Aging and caloric restriction. Course: Biological, Biomedical and Social Aspects of Aging. Course Director, Dr. Federico Sesti. Rutgers 16:761:610 spring 2018 March 2018 - Neuroscience Program, Advanced studies, 16:710:606:01, spring 2018, Three 2-hr class, Program Director, Dr. John Pintar. March 28, 2018: Topics: Mol Cell Biol "Survival skills”. Protein Structure Analysis/Comparison Tools class. Coordinators: Nancy Walworth and Rick Padgett, Co-Directors, Joint Graduate Programs in Molecular Biosciences March 30, 2018 - Topics in Advanced Biotechnology, Biotechnology graduate program. Title of the talk: Molecular aspects of synaptic connectivity”. September 2018 – M1 Histology laboratories, three labs, 2hrs each July – October 2019 – BIOL243 Human physiology course, School of Biological Sciences, VUW

B. Research Training 2007- UCSD Member, Proposition Exam Committee; Ms. Stephanie Nissen 2007- MS Thesis Co-Advisor: Ms. Beatrice Margani, School of Pharmacy, Bologna, Italy 2008- MS Thesis Co-Advisor: Mr. Simon Garcia, School of Pharmacy, Bologna, Italy 2009- MS Thesis Co-Advisor: Ms. Giulia Falivelli, School of Pharmacy, Bologna, Italy 2009-2010 MS Thesis Co-Advisor: Ms Trinh T Do Dept of Pharmacology, SSPPS, UCSD 2012- MS Thesis Co-Advisor: Mr. Francesco Bergami, School of Pharmacy, Bologna, Italy 2013- MS Thesis Co-Advisor: Mr. Francesca Martini, School of Pharmacy, Bologna, Italy 2014- PhD thesis Co-Advisor: Mr. Gabriele Vincelli, School of Pharmacy, Bologna, Italy 2014- PhD Thesis Co-Advisor: Ms. Giulia Falivelli, School of Pharmacy, Bologna, Italy 2014- MS Thesis Co-Advisor: Mr. Daniele Bertoglio, School of Pharmacy, Bologna, Italy 2015- MS Thesis Co-Advisor: Ms. Federica Raimondi, School of Pharmacy, Bologna, Italy 2015- MS Thesis Co-Advisor: Ms. Aula Hamod, Child Health Institute of New Jersey 2016 - MS Thesis Co-Advisor: Mr. Davide Sereni School of Pharmacy, Bologna, Italy 2017 - PhD Thesis Co-Advisor: Ms. Laura Trobiani, University “La Sapienza”, Rome, Italy 2018 - MS Thesis Co-Advisor: Ms. Flavia Betti School of Pharmacy, Bologna, Italy

Honor Undergraduate training May 2014 Irene Pakos May 2015 Ian Hagee May 2015 Nicole Mlynaryk (Pang Lab) May 2015 Julia Xia (Pang Lab) May 2017: Genevieve Conway April 2018: Ms. Paola Batarseh (Dr. Denzin) May 2018: Ms. Jacqueline Katz (Dr. Denzin)

GRANT SUPPORT: A. Principal Investigator Current:

1. National Science Foundation – IOS 08/2018 – 07/2021 Grant title: Collaborative Research: Structure and function of Reelin in brain development

2. 2017 Busch Biomedical Grant Program 09/1/17 – 08/31/19 Grant title: “Rapid and Unbiased Identification of Viral Entry Receptors in Human Neurons”

3. NJ Commission on Traumatic Brain Injury Research (PI) 7/1/2016 - 06/30/2019 Grant Title: Neuronal cell surface molecules that stimulate myelination Page 4 of 9

4. The Fred & Santa Barile Children’s Medical Research Trust (PI) 08/2016 – 12/2019 Grant Title: White Matter Myelination and the Role of CASPR2 in Autism.

Previous: 1. 2017 RUNEG 4/1/17 – 3/31/18 Grant Title: “An axon-free, in vitro model of central nervous system myelination 2. 2016 Precision Medicine Pilot Award (Co-PI) CINJ 08/2016 – 07/2018 Grant Title: Rapid and unbiased discovery of novel checkpoint inhibitor targets for immunotherapy 3. 2016 Century for the Cure funds (CINJ, administer by Dr. Roger Strair) 08/2016 – 07/2018 This additional funds are for the same Precision Medicine Pilot Award above 5. NJ Governor’s Council for Medical Research and Treatment of Autism: (Co-PI) 07/2016 – 06/2018 Grant Title: Identifying molecular players for synaptic formation deficits in human neurons from ASD subjects 4. RO1 grant # R01MH092906 Comoletti (PI) 08/2011 – 12/2016 Grant title: Caspr2 as an autism candidate gene: a proteomic approach to function & structure 5. NSF BRAIN EAGER, MCB-1450895 Comoletti PI, Montelione co-PI Grant title: the proteome of synaptogenesis. 09/2014 – 08/2016 6. NJ Governor’s Council for Medical Research and Treatment of Autism: (PI) 07/2014 – 06/2017 Grant title: “A human stem cell–based model to study autism-linked endoplasmic reticulum stress and initial testing of therapeutic strategies” 7. Aresty Research Center for Undergraduates and the School of Arts and Sciences. Undergraduate Research Fellowship to Sumie Kakehi ($1,000) academic year 2015-2016. 8. Aresty Research Center for Undergraduates and the School of Arts and Sciences. Undergraduate Research Fellowship to Irene Pakos ($950) and Zinal Chhangawala ($1,000). Winter and spring quarter 2012/2013. 9. Autism Speaks. A, Award #2617 “Neuroligins and Neurexins as Autism Candidate Genes: Study of their Association in Synaptic Connectivity”, 01/2009-06/2011, Total Costs: $120,000 10. Cure Autism Now (CAN), “Biochemical and Cellular Characterization of Neuroligin-3 Mutation Associted with Autism Spectrum Disorders, 01/2005-06/2007, Total Costs: $120,000 11. National Alliance of Autism Research, Grant Award #843, “Neuroligin and Autism Spectrum Disorder: Role of R451C Mutation in Neuroligin-3”, 07/2004-06/2006, Total Costs: $120,000

PUBLICATIONS: ORCID # 0000-0003-2081-6376

A. Refereed Original Article in Journal 1. Ranaivoson MR, Turk LS, Ozgul S, Kakehi S, von Daake S, Lopez N, Trobiani N, De Jaco A, Denissova N, Demeler D, Özkan E, Montelione GT, Comoletti D. (2019) A proteomic screen of neuronal cell surface molecules reveals IgLONs as structurally conserved interaction modules at the synapse (Structure, in press) 2. Ozgul S, von Daake S, Kakehi S, Sereni D, Denissova N, Hanlon C, Huang YJ, Everett JK, Yin C, Montelione GT, Comoletti D. (2019) An ELISA-Based Screening Platform for Ligand-Receptor Discovery. Methods Enzymol. 615:453-475. 3. Rice HC, de Malmazet D, Schreurs A, Frere S, Van Molle I, Volkov AN, Creemers E, Vertkin I, Nys J, Ranaivoson FM, Comoletti D, Savas JN, Remaut H, Balschun D, Wierda KD, Slutsky I, Farrow K, De Strooper B, de Wit J. (2019) Secreted amyloid-β precursor protein functions as a GABA(B)R1a ligand to modulate synaptic transmission. Science. 363(6423). 4. Trobiani L, Favaloro FL, Di Castro MA, Di Mattia M, Cariello M, Miranda E, Canterini S, De Stefano ME, Comoletti D, Limatola C, De Jaco A. (2018) UPR activation specifically modulates glutamate neurotransmission in the cerebellum of a mouse model of autism. Neurobiol Dis. S0969-9961:30515-1 5. Vieth JA, Das J, Ranaivoson FM, Comoletti D, Denzin LK, Sant’Angelo DB (2017) TCR  pairing controls recognition of CD1d ligands and directs the development of adipose tissue-specific NKT cells, Nature Immunology, 18(1):36-44 6. Jackson VA, Mehmood S, Chavent M, Roversi P, Carrasquero M, Del Toro D, Seyit-Bremer G, Ranaivoson FM, Comoletti D, Sansom MS, Robinson CV, Klein R, Seiradake E. (2016) Super-complexes of adhesion GPCRs and neural guidance receptors. Nat Commun. Apr 19;7:11184. 7. Rubio-Marrero EN, Vincelli G, Jeffries CM, Shaikh TR, Pakos I, Ranaivoson FM, von Daake S, Demeler B, De Jaco A, Perkins G, Ellisman MH, Trewhella J, Comoletti D. (2015) Structural characterization of the extracellular domain of CASPR2 and insights into its association with the novel ligand Contactin1. J Biol Chem. Mar 11; 291 (11):5788-802

Page 5 of 9

8. Ulbrich L, Favaloro FL, Trobiani L, Marchetti V, Patel V, Pascucci T, Comoletti D, Marciniak SJ, De Jaco A. (2016) Autism associated R451C mutation in Neuroligin3 leads to the activation of the unfolded protein response in a PC12 Tet-On inducible system. Biochem J. Feb 15;473(4):423-34 9. Ranaivoson FM, Liu Q, Martini F, Bergami F, von Daake S, Li S, Lee D, Demeler B, Hendrickson WA, Comoletti D. (2015) Structural and mechanistic insights into the latrophilin3 - FLRT3 complex that mediates glutamatergic synapse development. Structure, Sep 1;23(9):1665-77 10. Telese F., Ma Q., Montilla Perez P., Notani D., Oh S., Li W., Comoletti D., Ohgi K., Taylor H., and Rosenfeld MG. (2015) LRP8-Reelin-Regulated Neuronal Enhancer Signature Underlying Learning and Memory Formation. Neuron, 86:696-710 11. Lee GH, Chhangawala Z. von Daake S, Savas JN., Yates 3rd JR. Comoletti D., and D’Arcangelo G. (2014) Reelin induces Erk1/2 signaling in cortical neurons through a non-canonical pathway. JBC, 289:20307-17 12. Savas JN, De Wit J., Comoletti D. Zemla R., Ghosh A. Yates 3rd JA (2014) Ecto-Fc MS identifies ligand-receptor interactions through extracellular domain Fc fusion protein baits and shotgun proteomic analysis. Nature Methods, 9:2061-74 13. O’Sullivan ML, Martini F, von Daake S, Comoletti D, Ghosh A. (2014) LPHN3, a presynaptic adhesion-GPCR implicated in ADHD, regulates the strength of neocortical layer 2/3 synaptic input to layer 5. Neural Dev. 9:7-17 14. Taylor P, De Jaco A, Comoletti D, Miller M, Camp S. (2012) Cholinesterase Confabs and Cousins: Approaching Forty Years. Chem Biol Interact.:S0009-2797(12)00201-3 15. Falivelli G, De Jaco A, Favaloro FL, Kim H, Wilson J, Dubi N, Ellisman MH, Abrahams BS, Taylor P, Comoletti D. (2012) Inherited genetic variants in autism-related CNTNAP2 show perturbed trafficking and ATF6 activation. Hum Mol Genet. 21:4761-73 16. Suckow AT, Zhang C, Egodage S, Comoletti D, Taylor P, Miller MT, Sweet IR, Chessler SD. (2012) Transcellular neuroligin-2 interactions enhance insulin secretion and are integral to pancreatic β cell function. J Biol Chem. 287:19816-26. 17. O'Sullivan ML, de Wit J, Savas JN, Comoletti D, Otto-Hitt S, Yates JR 3rd, Ghosh A. (2012) FLRT proteins are endogenous latrophilin ligands and regulate excitatory synapse development. Neuron. 73:903-10. 18. De Jaco A, Comoletti D, Dubi N, Camp S, Taylor P. (2012) Processing of cholinesterase-like α/β-hydrolase fold proteins: alterations associated with congenital disorders. Protein Pept Lett. 2012 19:173-9. 19. Miller, MT., Mileni, M., Comoletti, D., Stevens, RC., Harel, M., Taylor, P.; (2011) The crystal structure of the α- neurexin-1 extracellular region reveals a hinge point for mediating synaptic adhesion and function; Structure 19:767-78 20. Comoletti, D., Miller, M., Jeffries, CM., Wilson, J., Demeler, B., Taylor, P., Trewhella, J., Nakagawa, T.; (2010) The macromolecular architecture of the extracellular domain of αNRXN1: domain organization, flexibility, and insights into trans-synaptic disposition; Structure, 18:1044-1053; 2010 21. De Jaco, A., Lin, MZ., Dubi, N., Comoletti, D., Miller, M., Camp, S., Ellisman, M., Butko, MT., Tsien, RY., Taylor, P.; (2010) Neuroligin trafficking deficiencies arising from mutations in the α/β-hydrolase fold protein family; J Biol Chem., 285:28674-82 22. Leone, P., Comoletti, D., Ferracci, G., Conrod, S., Garcia, SU., Taylor, P., Bourne, Y., Marchot, P.; (2010) Structural insights into the exquisite selectivity of neurexin/neuroligin synaptic interactions; EMBO Journal, 29:2461-71 23. Wang, X., Lee, J., Di Jeso, B., Treglia, A.S., Comoletti, D., Dubi, N., Taylor P., Arvan, P.; (2010) Cis and trans actions of the cholinesterase-like domain within the thyroglobulin dimer; J Biol Chem., 285:17564-73 24. De Wit, J., Sylwestrak, E., O’Sullivan, M., Otto, S., Tiglio, K., Savas, JN., Yates III, J., Comoletti, D., Taylor, P., and Ghosh, A.; (2009) LRRTM2 interacts with Neurexin1 and Regulates Excitatory Synapse Formation; Neuron, 64:799-806 25. Suckow, AT., Comoletti, D., Waldrop, M., Mosedale, M., Egodage, S., Taylor, P., Chessler, S.; (2008) Expression of neurexin, neuroligin and their cytoplasmic binding partners in the pancreatic β-cells and the involvement of neuroligin in insulin secretion; Endocrinology, 149:6006-6017; 26. Fabrichny, IP., Leone, P., Sulzenbacher, G., Comoletti, D., Miller, MT., Taylor, P., Bourne, Y., and Marchot, P.; (2007) Structural Analysis of the Synaptic Protein Neuroligin and its β-neurexin Complex: Determinants for Folding and Cell Adhesion; Neuron, 56:979–991; 27. Comoletti, D., Grishaev, A., Whitten, A., Tsigelny, I., Taylor, P., Trewhella, J.; (2007) Synaptic Arrangement of the Neuroligin/β-Neurexin Complex Revealed by X-ray and Neutron Scattering. Structure, 15:693-705; 28. De Jaco A., Comoletti, D., Kovarik, Z., Gaietta, G., Radic, Z., Lockridge, O., Ellisman, MH., Taylor, P.; (2006) A Mutation Linked With Autism Reveals a Common Mechanism of Endoplasmic Reticulum Retention for the α/β Hydrolase-Fold Protein Family; J Biol Chem., 281:9667-76 29. Comoletti, D., Flynn, RE., Boucard, AA., Jennings, LL., Shi, J., Tsigelny, I., Newlin, HR., Südhof, TC. and Taylor, P.; (2006) Gene Selection, Alternative Splicing, and Post-Translational Processing of Neuroligin Regulate -Neurexins; Biochemistry, 45:12816-27 Page 6 of 9

30. Boucard, AA., Chubykin,AA., Comoletti, D., Taylor, P., and Südhof, TC.; (2005) A Splice-Code for Trans-synaptic Cell Adhesion Mediated by Binding of Neuroligin 1 to α- and β-Neurexins; Neuron, 48:1-8; 31. Chubykin, AA., Liu ,X., Comoletti, D., Tsigelny, I., Taylor, P. and Südhof, TC.; (2005) Dissection of Synapse Induction by Neuroligins: Effect of a Neuroligin Mutation Associated with Autism; J Biol Chem., 280 (23): 22365; 32. Comoletti, D., De Jaco, A., Jennings, LL., Flynn, R., Gaietta, G., Tsigelny, I., Ellisman MH., and Taylor, P.; (2004) The Arg451Cys-Neuroligin-3 Mutation Associated with Autism Reveals a Defect in Protein Processing; J. Neurosci. 24:4889-4893 33. Hoffman, RC., Jennings, LL., Tsigelny, I., Comoletti, D., Flynn, R., Südhof, TC., and Taylor, P.; (2004) Structural Characterization of Recombinant Soluble Rat Neuroligin 1: Mapping of Secondary Structure and Glycosylation by Mass Spectrometry; Biochemistry, 43:1496-1506 34. Comoletti, D., Flynn, R., Jennings, LL., Chubykin, A., Matsumura, T., Hasegawa, H., Südhof, TC., Taylor, P.; (2003) Characterization of the Interaction of a Recombinant Soluble Neuroligin-1 With Neurexin-1β; J Biol Chem., 278:50497-505; 35. Comoletti, D., Muzio, V., Capobianco, A., Ravizza, T. Mennini, T.; (2001) Nitric Oxide Produced by non- Motoneuron Cells Enhances Rat Embryonic Motoneuron Sensitivity to Excitotoxins: Comparison in Mixed Neuron/Glia or Purified Cultures; J Neurol Sci., 192:61-9 36. Mennini, T., Cagnotto, A., Carvelli, L., Comoletti, D., Manzoni, C., Muzio, V., Rizzi, M., Vezzani, A.; (1999) Biochemical and Pharmacological Evidence of a Functional Role of AMPA Receptors in Motor Neuron Dysfunction in MND Mice; Eur J Neurosci., 11:1705-10 37. Ghezzi, P., Bernardini, R., Giuffrida, R., Bellomo, M., Manzoni, C., Comoletti, D., Di Santo, E., Benigni, F., Mennini, T.; (1998) Tumor Necrosis Factor is Increased in the Spinal Cord of an Animal Model of Motor Neuron Degeneration; Eur Cytokine Netw., 9:139-44 38. Mennini, T., Bastone, A., Crespi, D., Comoletti, D., Manzoni, C.; (1998) Spinal cord GLT-1 Glutamate Transporter and Blood Glutamic Acid Alterations in Motor Neuron Degeneration (MND) Mice; J Neurol Sci., 157:31-6;

B. Books, Monographs and Chapters 1. Taylor, P., De Jaco, A and Comoletti, D.; Neuroligins; In: Squire LR (ed.) Encyclopedia of Neuroscience; volume 6; pp. 493-496. Oxford: Academic Press, New York, NY; 2009 2. Taylor, P., Radic, Z., Talley, T.T., Nemecz, A., De Jaco, A., Comoletti, D., Fabrichny, I.P., Leone, P., Miller, M.T., Dubi, N., Fokin, V.V., Sharpless, K.B., Bourne, Y., & Marchot, P.; Retrogenomics - Proceeding from gene product back to the gene: applications in drug discovery and uncovering linkages in congenital disorders. [English - French abstract and captions]; In: Toxines et fonctions cholinergiques neuronales et non neuronales pp. 29-32; Coll. Rencontres en Toxinologie, Publications de la SFET, Châtenay-Malabry, France.; 2008

C. Patents Held N/A

D. Other Articles (Reviews, Editorials, etc.) In Journals; Chapters; Books; other Professional Communications

1. Ranaivoson FM, von Daake S, Comoletti D. (2016) Structural insights into Reelin function: present and future. Frontiers in Neuroscience. May 27;10:137 2. COMOLETTI D, Why PIs Can and Should Help Graduate Students Look Beyond Academia. 2016 Neuronline SfN http://neuronline.sfn.org/Articles/Program-Development/2016/Why-PIs-Can-and-Should-Help-Graduate-Students- Look-Beyond-Academia 3. Leone P, COMOLETTI D, Taylor P, Bourne Y, Marchot P.; Structure-function relationships of the α/β-hydrolase fold domain of neuroligin: A comparison with acetylcholinesterase; Chem Biol Interact., 187:49-55; 2010 4. De JacoA, Dubi N., COMOLETTI D, Taylor P.; Folding anomalies of neuroligin3 caused by a mutation in the α/β- hydrolase fold domain; Chemico-Biological Interactions; 187:56-8; 2010 5. COMOLETTI D., Grishaev A., Whitten, AE., Taylor P., and Trewhella J.; Characterization of the solution structure of a neuroligin/β-neurexin complex Chemico-Biological Interactions; 175:150-5; 2008 6. De Jaco A., COMOLETTI D., King CC., Taylor P.; Trafficking of cholinesterases and neuroligins mutant proteins: an association with autism; Chemico-Biological Interactions; 175:349-51; 2008 7. De Jaco A., Kovarik Z., COMOLETTI D., Jennings LL., Gaietta G., Ellisman MH., Taylor P.; A Single Mutation Near the C-terminus in α/β-Hydrolase Fold Protein Family Causes a Defect in Protein Processing. Chem Biol Interact.; 157-158:371-2; 2008 8. COMOLETTI D., Mennini T., Bastone A., Crespi D., Manzoni C.; Alterations in Blood Glutamic Acid Concentrations and Spinal Cord GLT-1 Glutamate Transporter in Motor Neuron Degeneration (MND) Mice; Eur. Neuropsychopharm; 7 S2:S293; 1997

Page 7 of 9

E. Abstracts Not Included

INVITED PRESENTATIONS: A. Scientific (Basic Science): 1. March 21st 2019 - New Zealand Synchrotron Group & The Australian Synchrotron Workshop - Victoria University Wellington – Title of the talk: SAXS: a tool to study the architecture of cell surface neuronal proteins 2. December 11th 2018 – The Biodiscovery Symposium, Victoria University of Wellington, Center for Biodiscovery, Structural and Functional Insights into Synaptic Connectivity 3. October 18th, 2017, The Center for Integrative Proteomics Research, IQB & CABM seminar series, Rutgers University. Title of the talk: Identification and Structural Characterization of IgLONs, a Family of Synaptic Adhesion Proteins. 4. September 27th, 2017 Baeza, Spain, Title of the talk: Structural and Functional Insights into Synaptic Connectivity. (September 23-27, 2017) "Synapse formation, specification, and elimination: From molecules to circuits” workshop organized by Dr. Thomas Südhof. 5. July 10th, 2017, The Neuronal Surfaceome in Circuit Formation: From Structure to Function; Location: Anzola D’Ossola, (VB) Italy; Opening remarks and meeting planning 6. July 11th, 2017, The Neuronal Surfaceome in Circuit Formation: From Structure to Function; Location: Anzola D’Ossola, (VB) Italy; Title of the talk: Structural and Functional Insights into Synaptic Connectivity. 7. January 5th, 2017, The Center for Integrative Proteomics Research, and the Institute for Quantitative Biomedicine Rutgers University. 4th annual interdisciplinary quantitative biology boot camp in single particle cryo-EM. Title of the talk: Assessing Monodispersity and Size, Small angle X-ray scattering (SAXS), a practical crash course. 8. June 7th 2016, Cornell High Energy Synchrotron Source CHESS Users' Meeting 2016, Ithaca, NY, Title of the talk: "A structural view of the neuronal cell adhesion proteins" 9. January 14, 2016: University of California, San Diego (La Jolla, CA). Toxins to Therapeutics: A Symposium in Honor of Palmer Taylor. Title of the talk: Synaptic connectivity: molecular aspects and implications in neurodevelopmental disorders 10. October 22nd, 2015: Northwestern University, Chicago (IL) Feinberg School of Medicine, host Dr. Jeff Savas. Title of the talk: Structural Insights Into The Lphn3/Flrt3-Mediated Synaptic Connectivity 11. September 18, 2015, Montclair State University, New Jersey autism center of excellence summit 2015; the unfolding story of autism research in NJ. Title of the talk: A human stem cell–based model to study autism- linked endoplasmic reticulum stress and initial testing of therapeutic strategies 12. July 16th, 2015: University “La Sapienza” Rome, Italy. Host: Dr. Antonella Da Jaco, Dept of Biology and Biotechnology Charles Darwin. Title of the talk: Inherited genetic variants in CNTNAP2 show perturbed trafficking and elevated ER stress 13. July 8th 2015: VIB Center for the Biology of Disease, Host Dr. Joris de Wit. Title of the talk: Structural Insights Into The Lphn3/Flrt3-Mediated Synaptic Connectivity 14. March 31st, 2015 The Autism Initiative, Center for Civic Leadership and Responsibility Camden County College NJ Title of the talk: Synaptic connectivity: molecular aspects and implications in neurodevelopmental disorders 15. December 4 2014; Rutgers RWJMS Basic Science Seminar Series. Title of the talk: Molecular Aspects of Synaptic Connectivity 16. October 23, 2014: 32nd Annual Autism Conference in Atlantic City, NJ. Workshop titled: “Updates on Genetic Research.” 17. October 7th 2014: Experience Rutgers: Central New Jersey Discovering Autism: Join President Robert Barchi for a reception with Rutgers University scientists who are at the forefront of cutting-edge autism research. 18. September 13, 2014, at CHINJ: Putting Together the Pieces: Autism Research Symposium Title of the talk: How Neurons Build Circuits: Molecular Studies in Neurodevelopmental Disorders 19. March 27 2014; RWJMS, Pediatric Grand Rounds talk. Title of the talk: Synaptic connectivity: molecular aspects and implications in neurodevelopmental disorders 20. April 23, 2014; Juniata college (Huntingdon, Pennsylvania), Dept. of Chemistry Title of the talk: Synaptic connectivity: molecular aspects and implications in neurodevelopmental disorders 21. 2013 NJMS/UMDNJ Newark track seminar series Cell Biology, Neuroscience & Physiology program (CBNP). February 4th 2013 Title of the talk: Inherited genetic variants in CNTNAP2 show perturbed trafficking and elevated ER stress 22. 2012 – Advances in Child Health – 2012 annual UMDNJ research symposium. June 14th, 2012. Title of the talk: Inherited genetic variants in autism-related CNTNAP2 show perturbed trafficking and elevated ER stress

Page 8 of 9

23. 2012 - 20th International Analytical Ultracentrifugation Conference, San Antonio, Texas, March 25th-30th, 2012 Title of the talk: Structural characterization of the extracellular domains of members of the neurexin superfamily. 24. 2011 - Brain Health Institute, Rutgers University: 10/26/11, talk tile: “Defining a molecular network of synaptic adhesion proteins: the case of neuroligins, neurexins, Caspr2, LRRTM2” 25. 2010 June 28th – Turin State University, Dept of Biochemistry. Title of the talk: Autism candidate genes neuroligin and neurexin: a proteomic approach to structure and adhesive function. 26. 2008, October 15th-18th Stanford Synchrotron Radiation Laboratory, Stanford University, Stanford, CA - SSRL/LCLS User's meeting. Title of the talk: Small-Angle X-ray and Neutron Scattering of the Neuroligin/Beta-Neurexin Complex Reveal Its Synaptic Arrangement 27. 2008, May 28 - 2008 GE Healthcare-Biacore San Diego Mini-Symposium, San Diego, CA, USA. Title of the talk: Characterization of the interaction of recombinant soluble neuroligin with neurexin. 28. 2008, May 15th – University of California Los Angeles, David Geffen School of Medicine. Title of the talk: Autism candidate genes neuroligin and neurexin: a proteomic approach to structure and adhesive function. 29. 2008, April 16th – IIT, Italian Institute of Technology, Genoa, Italy. Title of the talk: Autism candidate genes neuroligin and neurexin: a proteomic approach to structure and adhesive function. 30. 2008, April 14th : “Mario Negri” Institute for Pharmacological Research, Milan, Italy. Title of the talk: Autism candidate genes neuroligin and neurexin: a proteomic approach to structure and adhesive function 31. 2007, June 24-29 – Lecturer for “Genomics-Based Strategies in the Discovery of Novel Drug Targets” summer school, Bologna, Italy. Title of the talk: Autism candidate genes neuroligin and neurexin: a proteomic approach to structure and adhesive function. 32. 2007, May 6-10 Suzhou, China - The IXth International Meeting on Cholinesterases. Title of the talk: Small- Angle Solution Scattering of the Neuroligin/ -Neurexin Complex Reveals its Synaptic Arrangement. 33. 2006, August 5-9 San Diego, CA - 20th Symposium of The Protein Society, Title of the talk: Small-Angle Solution Scattering of the Neuroligin in Associated with Neurexin Reveals the Arrangement of the Complex 34. 2005, September 27th : “Mario Negri” Institute for Pharmacological Research, Milan, Italy. Title of the talk: Autism: The Molecular Basis of Social Behavior 35. 2004, August 31st National Center for Microscopy and Imaging Research, UCSD, La Jolla, CA. Title of the talk: Autism: The Molecular Basis of Social Behavior 36. 2003, February 17th , Marseille, France. Title of the talk: Characterization of the interaction of recombinant soluble neuroligins with neurexin-1β

Journal covers June 2007 Cover for the journal: Structure June 2011 Cover for the journal: Structure June 2012 Cover for the journal: Neuron February 2016, Cover for Biochemical Journal

Varia  12th October 2018: External referee for 1 Year meeting for the PhD student Christina Baggott, “Inhaled corticosteroid use in mild asthma: the difference between two treatment regimens”  23rd October 2018: Session chair for the “2018 Postgraduate Colloquium Honours & Masters (Part 1)”

Page 9 of 9