21.10.2014NUP155 (CT) antibody (pAb)

Rabbit Anti -Human/Mouse 155 (CT)

Instruction Manual

Catalog Number PK-AB718-4703 Synonyms NUP155 Antibody: Nucleoporin 155, complex 155 Description The nuclear pore complex (NPC) is a assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. One protein in this assembly is the nucleoporin (NUP)-155, which is localized symmetrically to both the nucleoplasmic and cytoplasmic faces of the NPC. NUP155 has been reported to interact with both NUP35 and the mRNA export factor Gle1, but the functions of these interactions are still unknown. NUP155 has also been shown to be required for NPC assembly and (NE) membrane fusion. NUP155 is recruited late in NE formation, suggesting that NUP155 defines an essential late step in NE assembly. NUP155 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP155 may be an important drug target in HIV treatment. At least two isoforms of NUP155 are known to exist. Quantity 100 µg Source / Host Rabbit Immunogen NUP155 antibody was raised against an 18 amino acid peptide from near the carboxy terminus of human NUP155. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse Specificity Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. NUP155 antibody can be used for detection of NUP155 by Western blot at 0.5 - 1 µg/mL. Images Available upon request. References Tran EJ and Wente SR. Dynamic nuclear pore complex: life on the edge. Cell 2006; 125:1041-53. Radu A, Blobel G and Wozniak RW. Nup155 is a novel nuclear pore complex protein that contains neither repetitive sequence motifs nor reacts with WGA. J. Cell Biol. 1993; 121:1-9. Hawryluk-Gara LA, Shibuya EK, and Wozniak RW. Vertebrate Nup53 interacts with the and is required for the assembly of a Nup93-containing complex. Mol. Biol. Cell 2005; 16:2382-94. Rayala HJ, Kendirgi F, Barry DM, et al. The mRNA export factor Gle1 interacts with the nuclear pore complex protein Nup155. Mol. Cell Proteomics 2004; 3:145-55. Images Available upon request. Related Products Cat.No. PK-AB718-4703P - NUP155 Peptide Cat. No. PK-AB718-1286 - P815 Cell Lysate

FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014