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WO 2018/009838 Al 11 January 2018 (11.01.2018) W !P O PCT
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2018/009838 Al 11 January 2018 (11.01.2018) W !P O PCT (51) International Patent Classification: Declarations under Rule 4.17: C12N 5/075 (2010.01) — as to applicant's entitlement to apply for and be granted a (21) International Application Number: patent (Rule 4.1 7(H)) PCT/US2017/041 155 — as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(Hi)) (22) International Filing Date: 07 July 2017 (07.07.2017) Published: — with international search report (Art. 21(3)) (25) Filing Language: English — before the expiration of the time limit for amending the (26) Publication Langi English claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) (30) Priority Data: — with sequence listing part of description (Rule 5.2(a)) 62/359,416 07 July 2016 (07.07.2016) US (71) Applicant: RUBIUS THERAPEUTICS, INC. [US/US]; 620 Memorial Dr #100W, Cambridge, MA 02139 (US). (72) Inventors; and (71) Applicants: HARANDI, Omid [US/US]; 39 Rowena Road, Newton, MA 02459 (US). KHANWALKAR, Ur- jeet [IN/US]; 2 11 Elm Street, Apt. 3, Cambridge, MA 02139 (US). HARIHARAN, Sneha [IN/US]; 18 Hamilton Road, Apt. 407, Arlington, MA 02472 (US). (72) Inventors: KAHVEJIAN, Avak; 2 Beverly Road, Arling ton, MA 02474 (US). MATA-FINK, Jordi; 8 Windsor Rd #1, Somerville, MA 02144 (US).DEANS, Robert, J.; 1609 Ramsgate Court, Riverside, CA 92506 (US). -
E-Content-Januaray (2021)
K.N.G.Arts College for women Department of Botany I B.SC ALLIED BOTANY E-content-Januaray (2021) S.NO E-CONENT 1 UNIT-I 2 UNIT-III 3 UNITIV 18K2ZAB3 ALLIED BOTANY: TAXONOMY, ANATOMY, EMBRYOLOGY, HORTICULTURE AND ECOLOGY UNIT-I: TAXONOMY General outline of Bentham and Hooker’s classification. Detailed study and economic importance of the families: Rutaceae, Leguminosae, Cucurbitaceae, Euphorbiaceae and Poaceae. UNIT-III: EMBRYOLOGY Structure of mature anther and Ovule, Types of ovule. Double fertilization. Development of dicot embryo. UNIT-IV: HORTICULTURE Scope and Importance of Horticulture. Propagation method: Cutting, layering and grafting. Bonsai technique UNIT – I Dr.A.Pauline Fathima Mary, Guest lecturer in Botany, K.N.G.Arts College for Women (A). Thanjavur. UNIT III & IV Dr.S.Gandhimathi & Dr.A.Pauline Fathima Mary , Guest lecturer in Botany, K.N.G.Arts College for Women (A). Thanjavur. REFERENCES 1. Pandey B.P., 2001, Taxonomy. Of Angiosperms,S.Chand & company.Ltd.Newdelhi. 2. Pandey B.P., 2015(Edn), Plant Taxonomy. New central Book Agency,pvt Lit,New Delhi. 3. Rajaram,P.allied Botany 1983.CollegeBook Center.Thanjavur. 4. Kumar,K.N.,1999.Introduction of Horticulture ,Rajalakshmi Publication,Nagerkoil. UNIT – I BENTHAM AND HOOKER'S CLASSIFICATION OF PLANTS The outline of Bentham and Hooker's classification of plants is given below. The seeded plants are divided into three classes ' Dicotyledonae,Gymnospermae and Monocotyledonae Bentham and Hooker's classification of plants t is a natural system of classification and is based on important characters of the plants. Even today this system is being followed in India, United Kingdom and several other Commonwealth countries. -
Rhodococcus Jostii Strain 8
Functional characterisation of alkane-degrading monooxygenases in Rhodococcus jostii strain 8 Jindarat Ekprasert A thesis submitted to the School of Environmental Sciences in fulfilment of the requirements for the degree of Doctor of Philosophy September 2014 University of East Anglia Norwich, UK i Contents List of figures viii List of tables xiii Declaration xv Acknowledgements xvi Abbreviations xvii Abstract xxi Chapter 1 introduction 1 1.1. Significance of alkanes in the environment 2 1.1.1. Chemistry of alkanes 2 1.2. The Rhodococcus genus 3 1.2.1. Common characteristics of Rhodococcus spp. 3 1.2.2. Rhodococcus spp. are capable of degrading gaseous alkanes 4 1.2.3. Potential applications of Rhodococcus in biotechnology 5 1.3. Bacterial enzymes responsible for alkane degradation 6 1.3.1. Integral membrane, non-heme iron alkane hydroxylases (AlkB) 6 1.3.2. Soluble di-iron monooxygenases (SDIMO) 8 1.3.2.1. SDIMO classification 10 1.3.2.2. Molecular genetics of SDIMOs 13 1.3.2.3. Mutagenesis of soluble methane monooxygenase 13 1.3.3. Cytochrome P450 alkane hydroxylases 14 3.3.1. Class I P450 14 3.3.2. Class II P450 (CYP52) 15 3.3.3. Class II P450 (CYP2E, CYP4B) 15 1.3.4. Membrane bound copper-containing (and possibly iron-containing) monooxygenases 15 1.4. Alkane metabolisms in Rhodococcus spp. 16 1.4.1. Aerobic metabolism of C2-C4 gaseous alkanes in bacteria 16 1.4.1.1. Ethane (C2H6) metabolism 16 1.4.1.2. Propane (C3H8) metabolism 17 ii 1.4.1.3. -
Phylogeny and Biogeorgraphy Of
PHYLOGENY AND BIOGEOGRAPHY OF WATERMELON [CITRULLUS LANATUS (THUNB.) MATSUM. & NAKAI] BASED ON CHLOROPLAST, NUCLEAR SEQUENCE AND AFLP MOLECULAR MARKER DATA Jiarong Liu A thesis submitted to the Graduate Faculty of Auburn University in Partial Fulfillment of the Requirements for the Degree of Master in Science Auburn, Alabama August 8, 2005 PHYLOGENY AND BIOGEOGRAPHY OF WATERMELON [CITRULLUS LANATUS (THUNB.) MATSUM. & NAKAI] BASED ON CHLOROPLAST, NUCLEAR SEQUENCE AND AFLP MARKER DATA Jiarong Liu Permission is granted to Auburn University to make copies of this thesis at its discretion, upon request of individuals or institutions and at their expense. The author reserves all the publication rights. Signature of Author Date of Graduation iii VITA Jiarong (Rona) Liu, daughter of Maosheng Liu and Hongmei Ye, was born on November 7, 1981 in Suzhou, Jiangsu Province, the People’s Republic of China. She graduated in June 1999 from Suzhou No.3 High School. She attended Yangzhou University, Yangzhou, Jiangsu Province, P. R. China in 1999 and graduated in June, 2003 with a Bachelor of Science in Horticulture. In August 2003, she entered Graduate school at Auburn University, Auburn, Alabama to pursue a Master of science degree in Horticulture. She was employed as a Graduate Research Assistant and Teaching Assistant during her graduate studies. iv THESIS ABSTRACT PHYLOGENY AND BIOGEOGRAPHY OF WATERMELON [CITRULLUS LANATUS (THUNB.) MATSUM. & NAKAI] BASED ON CHLOROPLAST, NUCLEAR SEQUENCE AND AFLP MARKER DATA Jiarong Liu Master of Science, July 12, 2005 (B.S., Yangzhou University, 2003) 75 Typed pages Directed by Fenny Dane Watermelons [Citrullus lanatus (Thunb.) Matsum. & Nakai], together with cucumbers, melons of various sorts, summer squashes, winter squashes and pumpkins are the principal food plants of the gourd family (Cucurbitaceae). -
KEGG Orthology-Based Annotation of the Predicted
Dunlap et al. BMC Genomics 2013, 14:509 http://www.biomedcentral.com/1471-2164/14/509 DATABASE Open Access KEGG orthology-based annotation of the predicted proteome of Acropora digitifera: ZoophyteBase - an open access and searchable database of a coral genome Walter C Dunlap1,2, Antonio Starcevic4, Damir Baranasic4, Janko Diminic4, Jurica Zucko4, Ranko Gacesa4, Madeleine JH van Oppen1, Daslav Hranueli4, John Cullum5 and Paul F Long2,3* Abstract Background: Contemporary coral reef research has firmly established that a genomic approach is urgently needed to better understand the effects of anthropogenic environmental stress and global climate change on coral holobiont interactions. Here we present KEGG orthology-based annotation of the complete genome sequence of the scleractinian coral Acropora digitifera and provide the first comprehensive view of the genome of a reef-building coral by applying advanced bioinformatics. Description: Sequences from the KEGG database of protein function were used to construct hidden Markov models. These models were used to search the predicted proteome of A. digitifera to establish complete genomic annotation. The annotated dataset is published in ZoophyteBase, an open access format with different options for searching the data. A particularly useful feature is the ability to use a Google-like search engine that links query words to protein attributes. We present features of the annotation that underpin the molecular structure of key processes of coral physiology that include (1) regulatory proteins of -
Modelling of the Melissa Artificial Ecosystem
ESTEC/CONTRACT 8125/88/NL/‘FG PRF 141315 Modelling of the MELiSSA artificial ecosystem Toward a structured model of the nitrifying compartment - Description of the respiratory chain of nitrifying organisms - Development of assumptions for the reverse electron flow in the respiratory electron transport chain - Stoichiometric description of the nitrification - Determination of KL~ for oxygen transfer limitation TECHNICAL NOTE 23.2 L. Poughon Laboratoire de Genie Chimique Biologique 63177 AUBIERE Cedex, FRANCE April 1995 Technical note 23.2 Toward a structured model of the nitrifying compartment T.N. 23.2: Modelling of the MELiSSA artificial ecosystem TOWARD A STRUCl-URJZD MODEL OF THE NITRIFYING COMPARTMENT L. Poughon. Laboratoire de Genie Chimique Biologique 63177 AUBIERE Cedex. France. INTRODUCTION In the MELiSSA loop the nitrifying compartment has the same function than the nitrifying process in the terrestrial ecosystem (figure 1) which is to provide an edible N-source for plants or micro-organisms (as Spirulines in the case of the MELiSSA loop). The ammoniflcation processes from organic waste (as for example the human waste faeces and urea) are performed in the MELiSSA loop by the 2 first compartments (liquefying and anoxygenic phototrophs compartments). It must be noted that there are some structural differences between the MELiSSA N-loop and the terrestrial ecosystem: l- the MELiSSA loop represent a very simplified part of the N loop encountered on earth; 2- the denitrification process (N mineral -> N2) or the N2 removing (N2 -> N mineral) are not considered 3- the sole N - source is N03- for Spirulina, it is NlQ+ for phototrophs and it is organic N for the crew. -
Relating Metatranscriptomic Profiles to the Micropollutant
1 Relating Metatranscriptomic Profiles to the 2 Micropollutant Biotransformation Potential of 3 Complex Microbial Communities 4 5 Supporting Information 6 7 Stefan Achermann,1,2 Cresten B. Mansfeldt,1 Marcel Müller,1,3 David R. Johnson,1 Kathrin 8 Fenner*,1,2,4 9 1Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600 Dübendorf, 10 Switzerland. 2Institute of Biogeochemistry and Pollutant Dynamics, ETH Zürich, 8092 11 Zürich, Switzerland. 3Institute of Atmospheric and Climate Science, ETH Zürich, 8092 12 Zürich, Switzerland. 4Department of Chemistry, University of Zürich, 8057 Zürich, 13 Switzerland. 14 *Corresponding author (email: [email protected] ) 15 S.A and C.B.M contributed equally to this work. 16 17 18 19 20 21 This supporting information (SI) is organized in 4 sections (S1-S4) with a total of 10 pages and 22 comprises 7 figures (Figure S1-S7) and 4 tables (Table S1-S4). 23 24 25 S1 26 S1 Data normalization 27 28 29 30 Figure S1. Relative fractions of gene transcripts originating from eukaryotes and bacteria. 31 32 33 Table S1. Relative standard deviation (RSD) for commonly used reference genes across all 34 samples (n=12). EC number mean fraction bacteria (%) RSD (%) RSD bacteria (%) RSD eukaryotes (%) 2.7.7.6 (RNAP) 80 16 6 nda 5.99.1.2 (DNA topoisomerase) 90 11 9 nda 5.99.1.3 (DNA gyrase) 92 16 10 nda 1.2.1.12 (GAPDH) 37 39 6 32 35 and indicates not determined. 36 37 38 39 S2 40 S2 Nitrile hydration 41 42 43 44 Figure S2: Pearson correlation coefficients r for rate constants of bromoxynil and acetamiprid with 45 gene transcripts of ECs describing nucleophilic reactions of water with nitriles. -
M Gerber Orcid.Org 0000-0002-5118-4087
Determining strategies of Acanthosicyos horridus (!nara) to exploit alternative atmospheric moisture sources in the hyper-arid Namib Desert M Gerber orcid.org 0000-0002-5118-4087 Dissertation submitted in fulfilment of the requirements for the degree Master of Science in Environmental Sciences at the North-West University Supervisor: Prof SJ Piketh Co-supervisor: Dr JM Berner Assistant Supervisor: Dr GL Maggs-Kölling Graduation May 2018 23387998 i ACKNOWLEDGEMENTS I would like to thank the following institutions and people for their contribution to my dissertation: • My sponsor and supervisor Prof. Stuart Piketh for the financial support; • North-West University for the financial support; • My supervisors Dr. Jacques Berner and Dr. Gillian Maggs-Kölling for their guidance and support; • Gobabeb Research and Training Centre (GTRC) for accommodating me for the last two years and for all the experience I gained working with them; • Dr. Eugene Marais, Dr. Mary Seely and Prof. Scott Turner for their advice and motivation; • Gobabeb Research and Training Staff members for their assistance and support; • Paulina Smidt for the translation and assisting me with fieldwork; • André Steyn and Elizabeth Shilunga for assisting me with fieldwork; • Oliver Halsey for the amazing pictures and assisting with the time-lapse investigation; • Esmé Harris for proofreading my dissertation; • My family and friends for their constant support. ii DECLARATION iii ABSTRACT The enigmatic melon species Acanthosicyos horridus Welw. ex Hook. f., locally known as !nara, is endemic to the hyper-arid Namib Desert where it occurs in sandy dune areas and dry river banks. The Namib Desert is a result of the cold Benguela current off the coast of Namibia. -
Comparison of Physiology and Genome-Wide Expression in Two Nitrosomonas Spp
Comparison of physiology and genome-wide expression in two Nitrosomonas spp. under batch cultivation By Mohammad Ghashghavi A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science In Microbiology and Biotechnology Department of Biological Sciences University of Alberta © Mohammad Ghashghavi, 2014 Abstract: Ammonia oxidizing bacteria (AOB) play a central role in the nitrogen cycle by oxidizing ammonia to nitrite. Nitrosomonas europaea ATCC 19718 has been the single most studied AOB that has contributed to our understanding of chemolithotrophic ammonia oxidation. As a closely related species, Nitrosomonas eutropha C91 has also been extensively studied. Both of these bacteria are involved in wastewater treatment systems and play a crucial part in major losses of ammonium-based fertilizers globally. Although comparative genome analysis studies have been done before, change in genome-wide expression between closely related organisms are scarce. In this study, we compared these two organisms through physiology and transcriptomic experiments during exponential and early stationary growth phase. We found that under batch cultivation, N. europaea produces more N2O while N. eutropha consumes more nitrite. From transcriptomic analysis, we also found that there are selections of motility genes that are highly expressed in N. eutropha during early stationary growth phase and such observation was completely absent in N. europaea. Lastly, principle homologous genes that have been well studied had different patterns of expression in these strains. This study not only gives us a better understanding regarding physiology and genome-wide expression of these two AOB, it also opens a wide array of opportunities to further our knowledge in understanding other closely related species with regards to their evolution, physiology and niche preference. -
Foraging Ecology and Conservation Biology of African Elephants: Ecological and Evolutionary Perspectives on Elephant-Woody Plant Interactions in African Landscapes
Foraging ecology and conservation biology of African elephants: Ecological and evolutionary perspectives on elephant-woody plant interactions in African landscapes Item Type Thesis Authors Dudley, Joseph Paine Download date 27/09/2021 15:01:40 Link to Item http://hdl.handle.net/11122/9523 INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter free, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back o f the book. Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6” x 9” black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. UMI A Bell & Howell Information Company 300 North Zed) Road, Ann Arbor MI 48106-1346 USA 313/761-4700 800/521-0600 Reproduced with permission of the copyright owner. -
Cucurbitaceae”
1 UF/IFAS EXTENSION SARASOTA COUNTY • A partnership between Sarasota County, the University of Florida, and the USDA. • Our Mission is to translate research into community initiatives, classes, and volunteer opportunities related to five core areas: • Agriculture; • Lawn and Garden; • Natural Resources and Sustainability; • Nutrition and Healthy Living; and • Youth Development -- 4-H What is Sarasota Extension? Meet The Plant “Cucurbitaceae” (Natural & Cultural History of Cucurbits or Gourd Family) Robert Kluson, Ph.D. Ag/NR Ext. Agent, UF/IFAS Extension Sarasota Co. 4 OUTLINE Overview of “Meet The Plant” Series Introduction to Cucubitaceae Family • What’s In A Name? Natural History • Center of origin • Botany • Phytochemistry Cultural History • Food and other uses 5 Approach of Talks on “Meet The Plant” Today my talk at this workshop is part of a series of presentations intended to expand the awareness and familiarity of the general public with different worldwide and Florida crops. It’s not focused on crop production. Provide background information from the sciences of the natural and cultural history of crops from different plant families. • 6 “Meet The Plant” Series Titles (2018) Brassicaceae Jan 16th Cannabaceae Jan 23rd Leguminaceae Feb 26th Solanaceae Mar 26th Cucurbitaceae May 3rd 7 What’s In A Name? Cucurbitaceae the Cucurbitaceae family is also known as the cucurbit or gourd family. a moderately size plant family consisting of about 965 species in around 95 genera - the most important for crops of which are: • Cucurbita – squash, pumpkin, zucchini, some gourds • Lagenaria – calabash, and others that are inedible • Citrullus – watermelon (C. lanatus, C. colocynthis) and others • Cucumis – cucumber (C. -
New Information on the Origins of Bottle Gourd (Lagenaria Siceraria)
New Information on the Origins of Bottle Gourd (Lagenaria siceraria) Item Type Article Authors Ellert, Mary Wilkins Publisher University of Arizona (Tucson, AZ) Journal Desert Plants Rights Copyright © Arizona Board of Regents. The University of Arizona. Download date 27/09/2021 04:31:57 Link to Item http://hdl.handle.net/10150/555919 8 Desert Plants of use by humans. Studies-both archeological and genetic, New Information on the Origins of seed and fruit-rind fragments indicate it had reached East of Bottle Gourd Asia 8,000 to 9,000 years before present (B.P.), that it was present as a domesticated plant in the New World by 10,000 (Lagenaria siceraria) B. P. and that it had a wide distribution in the Americas by 8,000 B.P. (Smith, 2000 and Erickson et. al, 2005) In the Southwestern US, bottle gourd most likely entered from Mary Wilkins Ellert Mexico as a domesticated plant at about the same time as 4433 W. Pyracantha Drive com (Zea mays) and squash (Cucurbita pepo)-by 3,500 to Tucson, Arizona 85741 4000 B.P.-and was widely grown as a container crop (Smith, [email protected] 2005). It is still grown today in the Sonoran Desert by the O'odam people, and seeds of the various traditional con tainer crop varieties are readily available through the Na " ........ always something new out of Africa" tive Seeds SEARCH group in Tucson, Arizona. History/Prehistory of Lagenaria In spite of its pan-tropical, pre-Columbian distribution, no Spanning continents, climates and cultures, the bottle gourd, evidence of the bottle gourd occurring in the wild on any Lagenaria siceraria (Mol.) Standley, Cucurbitaceae, has continent, as an indigenous part of the flora, rather than an served humans for thousands of years.