Isolation, Culture, and Behavior of Frankia Strain HFPCgI4 from Root Nodules of Casuarina glauca Author(s): Samira R. Mansour, Ahmed Dewedar, John G. Torrey Reviewed work(s): Source: Botanical Gazette, Vol. 151, No. 4 (Dec., 1990), pp. 490-496 Published by: The University of Chicago Press Stable URL: http://www.jstor.org/stable/2995335 . Accessed: 03/04/2012 15:56 Your use of the JSTOR archive indicates your acceptance of the Terms & Conditions of Use, available at . http://www.jstor.org/page/info/about/policies/terms.jsp JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact
[email protected]. The University of Chicago Press is collaborating with JSTOR to digitize, preserve and extend access to Botanical Gazette. http://www.jstor.org BOT. GAZ.151(4):490-496. 1990. (C)1990 by The Universityof Chicago. All rightsreserved. 0006-8071 /90/5 104-00 1 5$02 .00 ISOLATION,CULTURE, AND BEHAVIOROF FRANKIASTRAIN HFPCgI4 F1tOMROOT NODULES OF CASUARINAGLAUCA SAMIRAR. MANSOUR,'AHMED DEWEDAR,' AND JOHNG. TORREY HarvardForest, HarvardUniversity, Petersham, Massachusetts 01366 Casuarina glauca (Casuarinaceae)is an importantintroduced tree species in Egypt, valued for wind- breaks,land stabilization,and soil improvementassociated with actinomycete-inducedroot nodulesthat fix atmosphericnitrogen. A strainof Frankia designatedHFPCgI4 was isolatedfrom root nodulescollected in Egypt and its characteristicsassessed both in pure cultureand in symbiosis. StrainCgI4 grows well in syntheticnutrient medium with addedNH4+ or, in the absenceof combinedN in the medium,forms vesicles andfixes dinitrogenadequate for growth.Hyphae, vesicles, sporangia,and spores characteristic of the genus Frankia were observed.This strainshows spontaneousspore release when grown in medialacking N.