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Selective Antibiotics SELECTIVE ANTIBIOTICS The highest quality The best price Selection starts here Discover the most extensive line of selective antibiotics you’ll find anywhere. The selective InvivoGen provides high-quality and affordable antibiotics for selection with antibiotics unmatched purity levels. Manufactured in its state-of-the-art facility from its of choice own proprietary strains, all InvivoGen selective antibiotics are sterile, stable, Blasticidin S cell culture-tested and ready to use. G418 Sulfate Hygromycin B HygroGold™ Phleomycin Cell culture tested Puromycin Zeocin™ Rigorous quality control For more information go to www.invivogen.com Bulk quantities available T +33 (5) 62 71 69 39 F +33 (5) 62 71 69 30 E [email protected] www.invivogen.com Selection starts here PRODUCT WORKING CONC. STABILITY CONCENTRATION QUANTITY CAT CODE Blasticidin S Cells: 1-10 μg/ml >1 year at -20°C 10 mg/ml 100 mg ant-bl-1 E. coli: 25-100 μg/ml 1 year at 4°C 500 mg ant-bl-5 2 months at 20°C 500 mg (bottle) ant-bl-5b 2 weeks at 37°C 1 g (powder) ant-bl-10p G418 Sulfate Cells: 400-1000 μg/ml >1 year at -20°C 100 mg/ml 1 g ant-gn-1 1 year at 4°C 5 g ant-gn-5 1 month at 20°C 1 week at 37°C Hygromycin B Cells: 50-200 μg/ml >1 year at -20°C 100 mg/ml 1 g ant-hm-1 (purity >85%) E. coli: 100 μg/ml 1 year at 4°C 5 g ant-hm-5 HygroGold™ 3 months at 20°C 1 g ant-hg-1 (purity >98%) 12 weeks at 37°C 5 g ant-hg-5 10 g (powder) ant-hg-10p Phleomycin Yeasts: 10 μg/ml >1 year at -20°C 20 mg/ml 100 mg ant-ph-1 Filamentous 1 year at 4°C 500 mg ant-ph-5 Fungi: 25-150 μg/ml 6 months at 20°C 250 mg (powder) ant-ph-2p 4 weeks at 37°C 1 g (powder) ant-ph-10p Puromycin Cells: 1-10 μg/ml >1 year at -20°C 10 mg/ml 100 mg ant-pr-1 E. coli: 100 μg/ml 1 year at 4°C 500 mg ant-pr-5 3 months at 20°C 12 weeks at 37°C Zeocin™ Cells: 50-300 μg/ml >1 year at -20°C 100 mg/ml 1 g ant-zn-1 E. coli: 25 μg/ml 1 year at 4°C 5 g ant-zn-5 6 months at 20°C 1 g (powder) ant-zn-1p 4 weeks at 37°C 5 g (powder) ant-zn-5p For research purposes only. Not intended for use on animals or humans. HygroGold™ and Zeocin™ are trademarks of Cayla-InvivoGen. Blasticidin S – This nucleoside antibiotic Phleomycin – A glycopeptide antibiotic of Selecting E. coli transformants? specifically inhibits protein synthesis in both the bleomycin family, Phleomycin binds and Fast-Media® is the hassle-free way to prokaryotes and eukaryotes. Resistance is intercalates DNA in most aerobic cells, prepare E. coli selection media in just conferred by the blasticidin resistance gene, destroying the integrity of the double helix. five minutes with a microwave. bsr, derived from Bacillus cereus. Phleomycin is active against most bacteria, Our Fast-Media® are available in a wide variety of antibiotics. G418 Sulfate – Use this aminoglycoside filamentous fungi, yeast, plant and animal cells. antibiotic to block polypeptide synthesis Resistance is conferred by the Sh ble gene by inhibiting the elongation step in both from Streptoalloteichus hindustanus. prokaryotes and eukaryotes. Resistance Puromycin – This aminonucleoside antibiotic is conferred by the neo gene from Tn5. specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. It Hygromycin B and HygroGold™ – This aminoglycoside antibiotic, available at inhibits the growth of Gram positive bacteria two purity levels, inhibits protein synthesis and various animal and insect cells. Resistance by interfering with translocation and causing is conferred by the Pac gene. mistranslations at the 70S ribosome. It is Zeocin™ – A copper-chelated glycopeptide effective on most bacteria, fungi and higher antibiotic that causes cell death by intercalating eukaryotes. Resistance is conferred by into DNA and cleaving it. Effective on most the hph gene from E. coli. Hygromycin B aerobic cells, Zeocin™ is useful for selection in purity exceeds 85%; HygroGold™ purity bacteria, eukaryotic microorganisms, plant and exceeds 98%. animal cells. Resistance is conferred by the Sh ble gene. Cayla - InvivoGen T +33 (5) 62 71 69 39 F +33 (5) 62 71 69 30 E [email protected] www.invivogen.com.
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