Upstream Binding Protein 1 (UBP1) (NM 001128161) Human Tagged ORF Clone Product Data

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RC225928

Upstream Binding Protein 1 (UBP1) (NM_001128161) Human Tagged ORF Clone Product data:

Product Type: Expression Plasmids Product Name: Upstream Binding Protein 1 (UBP1) (NM_001128161) Human Tagged ORF Clone Tag: Myc-DDK Symbol: UBP1 Synonyms: LBP-1a; LBP-1B; LBP1A; LBP1B Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 4 Upstream Binding Protein 1 (UBP1) (NM_001128161) Human Tagged ORF Clone – RC225928

ORF Nucleotide >RC225928 ORF sequence Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC

ATGGCCTGGGTGCTCAAGATGGACGAGGTGATCGAGTCCGGGCTGGTGCACGACTTCGACGCCAGCCTCT CGGGCATCGGGCAGGAACTGGGCGCCGGCGCTTACAGCATGAGTGATGTCTTGGCATTGCCCATTTTCAA GCAGGAAGATTCCAGCCTTCCATTGGATGGTGAAACAGAGCACCCACCCTTTCAGTATGTGATGTGTGCT GCAACGTCACCAGCAGTAAAACTGCATGATGAAACGCTTACTTATTTGAACCAAGGTCAGTCATATGAAA TTCGGATGCTGGATAATCGGAAAATGGGTGATATGCCTGAGATCAATGGAAAATTAGTAAAGAGCATCAT AAGGGTTGTATTCCATGACAGACGGCTACAATACACAGAGCATCAGCAACTTGAAGGATGGAAGTGGAAT CGCCCAGGAGACAGACTTCTTGATTTAGATATTCCAATGTCTGTGGGAATAATTGACACAAGGACGAATC CAAGCCAGTTAAATGCGGTTGAATTTCTGTGGGACCCAGCAAAACGCACCTCTGCTTTCATTCAGGTACA CTGCATCAGCACAGAATTTACTCCACGGAAGCACGGAGGTGAAAAGGGAGTGCCCTTTAGGATCCAGGTT GACGCCTTTAAGCAGAATGAAAATGGAGAATACACAGATCATCTACACTCAGCTAGCTGCCAAATCAAAG TTTTTAAGCCTAAAGGTGCAGACAGGAAACAAAAAACTGACCGAGAGAAGATGGAGAAGAGAACAGCTCA TGAAAAAGAAAAGTATCAGCCGTCCTATGATACCACAATCCTCACAGAGATGAGGCTTGAGCCTATAATT GAAGATGCAGTTGAACATGAGCAGAAAAAGTCCAGCAAGCGGACTTTGCCAGCAGACTACGGTGATTCTC TGGCAAAGCGAGGCAGTTGTTCTCCGTGGCCCGATGCCCCCACAGCCTATGTGAATAACAGCCCTTCCCC AGCGCCCACTTTCACCTCCCCACAGCAGAGCACTTGCAGTGTCCCAGACAGCAATTCTTCTTCCCCAAAT CATCAGGGAGATGGAGCTTCACAGACCTCTGGTGAACAAATTCAGCCTTCAGCTACGATCCAGGAAACAC AGCAATGGCTGCTCAAAAACAGATTCTCTTCCTACACAAGACTGTTCTCTAATTTTTCAGGTGCCGACTT ATTAAAACTGACAAAGGAGGATTTAGTTCAAATTTGTGGTGCAGCCGATGGAATTCGGCTCTATAATTCA CTGAAGTCAAGGTCGGTTAGACCCCGTTTAACCATCTATGTCTGCCGGGAGCAGCCAAGCAGCACAGTGC TGCAAGGGCAGCAGCAAGCTGCAAGCAGTGCAAGCGAGAATGGCAGTGGGGCACCCTATGTTTATCATGC AATCTACTTGGAAGAAATGATTGCCTCAGAAGTTGCTCGAAAACTTGCGCTGGTGTTTAATATCCCTCTC CACCAAATTAATCAGGTTTACAGACAGGGTCCCACCGGTATTCACATTCTTGTTAGTGATCAGATGGTTC AGAATTTTCAAGATGAGAGTTGTTTTTTATTCTCCACAGTGAAAGCTGAAAGTAGTGATGGCATCCACAT AATTTTGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA

Protein Sequence: >RC225928 protein sequence Red=Cloning site Green=Tags(s)

MAWVLKMDEVIESGLVHDFDASLSGIGQELGAGAYSMSDVLALPIFKQEDSSLPLDGETEHPPFQYVMCA ATSPAVKLHDETLTYLNQGQSYEIRMLDNRKMGDMPEINGKLVKSIIRVVFHDRRLQYTEHQQLEGWKWN RPGDRLLDLDIPMSVGIIDTRTNPSQLNAVEFLWDPAKRTSAFIQVHCISTEFTPRKHGGEKGVPFRIQV DAFKQNENGEYTDHLHSASCQIKVFKPKGADRKQKTDREKMEKRTAHEKEKYQPSYDTTILTEMRLEPII EDAVEHEQKKSSKRTLPADYGDSLAKRGSCSPWPDAPTAYVNNSPSPAPTFTSPQQSTCSVPDSNSSSPN HQGDGASQTSGEQIQPSATIQETQQWLLKNRFSSYTRLFSNFSGADLLKLTKEDLVQICGAADGIRLYNS LKSRSVRPRLTIYVCREQPSSTVLQGQQQAASSASENGSGAPYVYHAIYLEEMIASEVARKLALVFNIPL HQINQVYRQGPTGIHILVSDQMVQNFQDESCFLFSTVKAESSDGIHIILK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV Chromatograms: https://cdn.origene.com/chromatograms/mk6513_c01.zip Restriction Sites: SgfI-MluI

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 4 Upstream Binding Protein 1 (UBP1) (NM_001128161) Human Tagged ORF Clone – RC225928

Cloning Scheme:

Plasmid Map:

ACCN: NM_001128161

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 3 / 4 Upstream Binding Protein 1 (UBP1) (NM_001128161) Human Tagged ORF Clone – RC225928

ORF Size: 1620 bp OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_001128161.1, NP_001121633.1 RefSeq Size: 3781 bp RefSeq ORF: 1623 bp Locus ID: 7342 UniProt ID: Q9NZI7, A8KAN5 Protein Families: Transcription Factors MW: 60.5 kDa Gene Summary: Functions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2 (By similarity). Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA- box.[UniProtKB/Swiss-Prot Function]

Product images:

Western blot validation of overexpression lysate (Cat# [LY426903]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un- transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225928 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).