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Winlist User Guide

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Winlist User Guide WinList User Guide Copyright 2016 Verity Software House All rights reserved. Updated 22 July 2016 This guide is intended for WinList 9.0 and minor version updates. Table of Contents Welcome ..........................................................................................................................13 License and Warranty ........................................................................................................15 Latest Versions .................................................................................................................17 What's new? .....................................................................................................................19 System Requirements ........................................................................................................69 Installing and Running WinList ...........................................................................................71 Registering Your Software .................................................................................................73 Using the Network Version .................................................................................................77 Contacting Verity Software House.......................................................................................83 Tutorials ...........................................................................................................................85 WinList Basics ...................................................................................................................85 Cascade Gating and Boolean Gates .....................................................................................94 Compensation the Easy Way: V-Comp .............................................................................. 103 Using Interactive N-Color Compensation ™ ....................................................................... 108 Compensation Review Mode ............................................................................................ 119 Creating Reports ............................................................................................................. 127 Using Overlays ................................................................................................................ 141 Using FCOM to enumerate phenotypes ............................................................................. 147 Cluster Identification with t-SNE ....................................................................................... 155 Sticky Regions and Magic Contours ................................................................................... 169 Kinetic Analysis with Excel ............................................................................................... 178 Enhanced Proliferation Analysis ........................................................................................ 188 Remote connection to ModFit LT ...................................................................................... 200 Using MultiGraphs ........................................................................................................... 203 Reference ....................................................................................................................... 207 Main Window .................................................................................................................. 207 Ribbon Bar ..................................................................................................................... 209 Toolbar .......................................................................................................................... 215 Status Bar ...................................................................................................................... 217 Protocols ........................................................................................................................ 218 Open Protocol ................................................................................................................. 218 Open Bundle................................................................................................................... 219 Save Protocol ................................................................................................................. 220 Save Protocol As... .......................................................................................................... 221 Save Bundle ................................................................................................................... 223 Files and Data Sources .................................................................................................... 224 Data Source Windows ..................................................................................................... 224 Data Source Toolbar........................................................................................................ 226 Data Source Context Menu .............................................................................................. 228 Open FCS File ................................................................................................................. 230 Open Dialog Box ............................................................................................................. 231 Choose Dataset............................................................................................................... 232 Next FCS File .................................................................................................................. 233 Previous FCS File ............................................................................................................ 234 Duplicate Data Source ..................................................................................................... 235 Edit Data Source ............................................................................................................. 236 Add Parameters .............................................................................................................. 238 Edit Calculated Parameter ................................................................................................ 240 FCOM Background and Usage .......................................................................................... 244 Edit Transformations ....................................................................................................... 246 Names and Resolutions ................................................................................................... 249 Quantitation ................................................................................................................... 251 Using the Quantitation Spreadsheet .................................................................................. 253 Compensation ................................................................................................................. 255 Compensation Toolbox .................................................................................................... 255 Compensation Toolbox dialog........................................................................................... 256 Compensation: Setup Tab ................................................................................................ 258 Compensation: AutoComp Tab ......................................................................................... 260 Compensation: Transforms Tab ........................................................................................ 262 Compensation: Miscellaneous Tab .................................................................................... 264 Compensation Trace Lines ............................................................................................... 266 Compensation Notice dialog ............................................................................................. 267 Edit Properties for Compensation ...................................................................................... 268 Advice on Compensation dialog ........................................................................................ 271 Normalization Targets ..................................................................................................... 272 Histograms ..................................................................................................................... 275 Histogram Windows ........................................................................................................ 275 Histogram Toolbar .......................................................................................................... 277 Histogram Context Menu ................................................................................................. 279 Create Histogram ............................................................................................................ 282 Create Multiple Histograms .............................................................................................. 284 Edit Histogram ................................................................................................................ 286 Edit Remote ................................................................................................................... 288 Edit 1P Graphics.............................................................................................................
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