hGPR25 VersaClone cDNA Catalog Number: RDC0310

Specifications: Description This shuttle vector contains the complete ORF for the gene of interest, Gene: hGPR25 along with a Kozak consensus sequence for optimal translation initiation. It is inserted NotI to AscI. The gene insert is flanked with Accession: NP_005289 convenient multiple cloning sites which can be used to easily cut and transfer the gene cassette into your desired expression vector. Insert size: 1099bp Preparation and Storage Concentration: 10µg at 0.2μg/μL Formulation cDNA is provided in 10 mM Tris-Cl, pH 8.5

Shipping Ships at ambient temperature

Stability 1 year from date of receipt when stored at -20°C to -80°C Storage Use a manual defrost freezer and avoid repeated hGPR25 cDNA freeze-thaw cycles.

Plasmid

GPR25 G protein-coupled PfoI NdeI receptor 25 [ Homo sapiens ] BbeI ZraI KasI AatII NarI BmgBI SspI SfoI HpaI Also known as: GPR-25 HindIII XmnI BamHI EcoRV BmtI ScaI PasI Summary: PpuMI NheI TsoI NotI GPR25 is an orphan receptor NcoI StyI belonging to the G protein- BsgI coupled receptor family. GPR25 AMP BtgZI is homologous to receptors FseI involved in BP regulation. It BsaI RDC0310 NaeI NgoMIV shares highest identity to the hGPR25 (1-361) AhdI 3833 bps receptor encoded by GPR15, BbvCI angiotensin II type 1A receptor, Bpu10I BsaAI and somatostatin receptor 5. PmlI AleI GPR25 is expressed in liver and BbsI brain. COLE1 AarI SrfI AscI Bst1107I EcoNI XbaI SalI ApoI PciI SapI EcoRI Eco47III

FOR RESEARCH USE ONLY

NOT FOR USE IN HUMANS

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> RDC0310 Plasmid DNA Sequence 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 101 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 201 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt 401 ggagacgtgt taacaagctt ggatccgata tcgctagcgc ggccgccacc atggccccca cagagccctg gagccccagc ccggggtcag cgccctggga 501 ctactcgggg ttggacggcc tggaggagct ggagctgtgt ccggccgggg acctgcccta cggctacgtc tacatccccg cgctctacct ggcggccttc 601 gccgtgggcc tgctgggcaa cgcctttgtg gtgtggctgc tggccgggcg gcggggcccg cggcggctgg tggatacctt cgtgctgcac ctggcggcag 701 ctgacctggg cttcgtgctc acgctgccgc tgtgggccgc ggcggcggcg ctaggaggac gatggccgtt cggcgatggc ctctgcaagc tcagcagctt 801 cgcgctggcg ggcacgcgct gcgcgggcgc gctgctgctg gcgggcatga gcgtggaccg ctacctggcc gtggtgaagc tgctcgaggc gaggccactg 901 cgcaccccgc gctgcgcgct ggcctcgtgc tgcggcgtct gggccgtggc gctgctggcc ggcctgccct ccctggtcta ccgggggttg cagcccctgc 1001 ctgggggcca ggacagccag tgcggcgagg agccctccca cgccttccag ggcctcagct tgctgctgct gctgctgacc ttcgtgctgc ccctggtcgt 1101 caccctcttc tgctactgcc gcatctcgcg ccgcctgcga cggccgccgc acgtgggtcg ggcccggagg aactcgctgc gcatcatctt cgccatcgag 1201 agcacgtttg tgggctcctg gctgcccttc agcgccctgc gggccgtctt ccacctggcg cgtctggggg cgctgccgct gccgtgcccc ctgctgctgg 1301 cgctgcgctg gggcctcacc attgccacct gcctggcctt cgtcaacagc tgcgccaacc cgctcatcta cctcctgctg gaccgctcat tccgagcccg 1401 ggcgctggac ggggcctgcg ggcgcaccgg ccgcctggcg cgaaggatca gctcagcctc ctcgctctcc agggacgaca gttccgtgtt ccgttgccgg 1501 gcccaggccg cgaacactgc ctcggcctcc tggtaaaggc gcgccagtat actctagagt cgacacccgg ggaattcctc gagcgctcgt ctctagcttg 1601 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 1701 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 1801 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 1901 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 2001 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 2101 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 2201 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 2301 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 2401 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 2501 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 2601 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 2701 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 2801 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 2901 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 3001 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 3101 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 3201 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 3301 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 3401 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 3501 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 3601 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 3701 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 3801 ataaaaatag gcgtatcacg aggccctttc gtc

> RDC0310 Translated Insert Sequence 1 maptepwsps pgsapwdysg ldgleelelc pagdlpygyv yipalylaaf avgllgnafv vwllagrrgp rrlvdtfvlh laaadlgfvl tlplwaaaaa 101 lggrwpfgdg lcklssfala gtrcagalll agmsvdryla vvkllearpl rtprcalasc cgvwavalla glpslvyrgl qplpggqdsq cgeepshafq 201 glsllllllt fvlplvvtlf cycrisrrlr rpphvgrarr nslriifaie stfvgswlpf salravfhla rlgalplpcp lllalrwglt iatclafvns 301 canpliylll drsfrarald gacgrtgrla rrissassls rddssvfrcr aqaantasas w

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