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Cross-Amplification of Nuclear Microsatellite Markers in Two
Braz. J. Bot (2017) 40(2):475–480 DOI 10.1007/s40415-017-0362-7 ORIGINAL ARTICLE Cross-amplification of nuclear microsatellite markers in two species of Cryptanthus Otto & A. Dietr. (Bromeliaceae) 1 2 3 De´bora Maria Cavalcanti Ferreira • Jordana Neri • Clarisse Palma-Silva • 4 5 4 Diego Sotero Pinange´ • Ana Maria Benko-Iseppon • Rafael Batista Louzada Received: 1 June 2016 / Accepted: 16 January 2017 / Published online: 27 January 2017 Ó Botanical Society of Sao Paulo 2017 Abstract Thirty-eight nuclear microsatellite loci origi- Keywords Bromeliad Á Cryptanthus burle-marxii Á nally developed for Aechmea caudata Lindm., Orthophy- Cryptanthus zonatus Á Microsatellite Á Transferability tum ophiuroides Louzada & Wand., Pitcairnia albiflos Herb., Vriesea gigantea (Gaud.) and V. simplex (Vell.) Beer were tested in Cryptanthus burle-marxii Leme and C. Introduction zonatus (Vis.) Vis. Of the 38 loci tested, 13 were poly- morphic. Ten polymorphic microsatellite loci were selec- The genus Cryptanthus Otto & A. Dietr. (Bromeliaceae) ted to be amplified and genotyped in one population each comprises 78 species restricted to Brazil, where they occur of C. burle-marxii and C. zonatus. The observed and in the Atlantic Forest, ‘‘Caatinga’’ and ‘‘Cerrado’’ (Forzza expected heterozygosity per locus in the C. burle-marxii et al. 2016). These species belong to the Bromelioideae population ranged from 0.050 to 0.850 and 0.050 to 0.770, subfamily and are terrestrial and/or saxicolous herbs respectively. In C. zonatus, the observed and expected (Ramı´rez-Morillo 1996). Traditionally Cryptanthus has heterozygosity per locus ranged from 0.167 to 0.846 and been recognized as sister group to the genus Orthophytum 0.290 to 0.692, respectively. -
Physiological Responses by Billbergia Zebrina (Bromeliaceae) When Grown Under Controlled Microenvironmental Conditions
Vol. 15(36), pp. 1952-1961, 7 September, 2016 DOI: 10.5897/AJB2016.15584 Article Number: 3D3064E60382 ISSN 1684-5315 African Journal of Biotechnology Copyright © 2016 Author(s) retain the copyright of this article http://www.academicjournals.org/AJB Full Length Research Paper Physiological responses by Billbergia zebrina (Bromeliaceae) when grown under controlled microenvironmental conditions João Paulo Rodrigues Martins1*, Veerle Verdoodt2, Moacir Pasqual1 and Maurice De Proft2 1Tissue Culture Laboratory of the Department of Agriculture at Federal University of Lavras, 37200-000, Lavras, Minas Gerais, Brazil. 2Division of Crops Biotechnics, Department of Biosystems, Katholieke Universiteit Leuven, Willem de Croylaan 42, box 2427, 3001 Leuven, Belgium. Received 24 July, 2016; Accepted 26 August, 2016 Sucrose, the most commonly used carbon source in conventional in vitro culture, and limited air exchange in the culture containers are factors that affect the growth of in vitro-cultured plants. They may induce physiological disorders and decrease the survival rate of plants after transfer to ex vitro conditions. The aim of the present study was to analyze the effects of gas exchange and sucrose concentration on Billbergia zebrina plantlets during in vitro propagation. In vitro-established B. zebrina plantlets were transferred to culture media containing 0, 15, 30, 45, or 60 g L-1 sucrose. Two different culture-container sealing systems were compared: lids with a filter (permitting gas exchange) and lids with no filter (blocking fluent gas exchange). Carbohydrate and chlorophyll (Chl a+b) concentrations were analyzed in plantlets at 45-days of culture. The addition of sucrose to the medium reduced the Chl a+b concentration in the plantlets. -
Functional Structure of the Bromeliad Tank Microbiome Is Strongly Shaped by Local Geochemical Conditions
Environmental Microbiology (2017) 19(8), 3132–3151 doi:10.1111/1462-2920.13788 Functional structure of the bromeliad tank microbiome is strongly shaped by local geochemical conditions Stilianos Louca,1,2* Saulo M. S. Jacques,3,4 denitrification steps, ammonification, sulfate respira- Aliny P. F. Pires,3 Juliana S. Leal,3,5 tion, methanogenesis, reductive acetogenesis and 6 1,2,7 Angelica L. Gonzalez, Michael Doebeli and anoxygenic phototrophy. Overall, CO2 reducers domi- Vinicius F. Farjalla3 nated in abundance over sulfate reducers, and 1Biodiversity Research Centre, University of British anoxygenic phototrophs largely outnumbered oxy- Columbia, Vancouver, BC, Canada. genic photoautotrophs. Functional community 2Department of Zoology, University of British Columbia, structure correlated strongly with environmental vari- Vancouver, BC, Canada. ables, between and within a single bromeliad species. 3Department of Ecology, Biology Institute, Universidade Methanogens and reductive acetogens correlated Federal do Rio de Janeiro, Rio de Janeiro, Brazil. with detrital volume and canopy coverage, and exhib- 4Programa de Pos-Graduac ¸ao~ em Ecologia e ited higher relative abundances in N. cruenta.A Evoluc¸ao,~ Universidade Estadual do Rio de Janeiro, Rio comparison of bromeliads to freshwater lake sedi- de Janeiro, Brazil. ments and soil from around the world, revealed stark differences in terms of taxonomic as well as func- 5Programa de Pos-Graduac ¸ao~ em Ecologia, tional microbial community structure. Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. 6Biology Department & Center for Computational & Introduction Integrative Biology, Rutgers University, Camden, NJ, USA. Bromeliads (fam. Bromeliaceae) are plants found through- 7Department of Mathematics, University of British out the neotropics, with many species having rosette-like Columbia, Vancouver, BC, Canada. -
Functional Structure of the Bromeliad Tank Microbiome Is Strongly Shaped by Local Geochemical Conditions
Environmental Microbiology (2017) 19(8), 3132–3151 doi:10.1111/1462-2920.13788 Functional structure of the bromeliad tank microbiome is strongly shaped by local geochemical conditions Stilianos Louca,1,2* Saulo M. S. Jacques,3,4 denitrification steps, ammonification, sulfate respira- Aliny P. F. Pires,3 Juliana S. Leal,3,5 tion, methanogenesis, reductive acetogenesis and 6 1,2,7 Angelica L. Gonzalez, Michael Doebeli and anoxygenic phototrophy. Overall, CO2 reducers domi- Vinicius F. Farjalla3 nated in abundance over sulfate reducers, and 1Biodiversity Research Centre, University of British anoxygenic phototrophs largely outnumbered oxy- Columbia, Vancouver, BC, Canada. genic photoautotrophs. Functional community 2Department of Zoology, University of British Columbia, structure correlated strongly with environmental vari- Vancouver, BC, Canada. ables, between and within a single bromeliad species. 3Department of Ecology, Biology Institute, Universidade Methanogens and reductive acetogens correlated Federal do Rio de Janeiro, Rio de Janeiro, Brazil. with detrital volume and canopy coverage, and exhib- 4Programa de Pos-Graduac ¸ao~ em Ecologia e ited higher relative abundances in N. cruenta.A Evoluc¸ao,~ Universidade Estadual do Rio de Janeiro, Rio comparison of bromeliads to freshwater lake sedi- de Janeiro, Brazil. ments and soil from around the world, revealed stark differences in terms of taxonomic as well as func- 5Programa de Pos-Graduac ¸ao~ em Ecologia, tional microbial community structure. Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. 6Biology Department & Center for Computational & Introduction Integrative Biology, Rutgers University, Camden, NJ, USA. Bromeliads (fam. Bromeliaceae) are plants found through- 7Department of Mathematics, University of British out the neotropics, with many species having rosette-like Columbia, Vancouver, BC, Canada. -
An Illustrated Checklist of Bromeliaceae from Parque Estadual Do Rio Preto, Minas Gerais, Brazil, with Notes on Phytogeography and One New Species of Cryptanthus
Phytotaxa 10: 1–16 (2010) ISSN 1179-3155 (print edition) www.mapress.com/phytotaxa/ Article PHYTOTAXA Copyright © 2010 • Magnolia Press ISSN 1179-3163 (online edition) An illustrated checklist of Bromeliaceae from Parque Estadual do Rio Preto, Minas Gerais, Brazil, with notes on phytogeography and one new species of Cryptanthus LEONARDO M. VERSIEUX1, RAFAEL B. LOUZADA2,4, PEDRO LAGE VIANA3, NARA MOTA3 & MARIA DAS GRAÇAS LAPA WANDERLEY4 1Universidade Federal do Rio Grande do Norte, Departamento de Botânica, Ecologia e Zoologia, 59072-970, Natal, Rio Grande do Norte, Brazil. E-mail: [email protected] 2Programa de Pós-Graduação em Ciências (Botânica), Instituto de Biociências, Universidade de São Paulo, Brazil E-mail: [email protected] 3Programa de Pós-Graduação em Biologia Vegetal, Instituto de Ciências Biológicas, Departamento de Botânica, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901, Belo Horizonte, Minas Gerais, Brazil. E-mail: [email protected], [email protected] 4Instituto de Botânica, Av. Miguel Estéfano 3687, 04301-012, São Paulo, São Paulo, Brazil. E-mail: [email protected] Abstract A checklist of the 14 genera and 34 species of Bromeliaceae from the Parque Estadual do Rio Preto in São Gonçalo do Rio Preto municipality, Minas Gerais state, southeastern Brazil, is presented. The Tillandsioideae was the most diverse subfamily and was found to be concentrated in rocky field areas. Bromelioideae is also a species rich subfamily, but its taxa have shown a preference to forested areas and savannas at lower altitudes. Pitcairnioideae is highlighted by its level of endemism, but has only four species. Cryptanthus micrus, a new species found in this area is described and illustrated. -
Entomofauna Em Duas Espécies De Bromélias No Oeste De Santa Catarina, Brasil
ISSN 1983-0572 Publicação do Projeto Entomologistas do Brasil www.ebras.bio.br Entomofauna em Duas Espécies de Bromélias no Oeste de Santa Catarina, Brasil Mario Arthur Favretto¹, Marcia Patrícia Hoeltgebaum², Rodrigo Lingnau³ & Fernanda Maurer D’Agostini¹ 1. UNOESC - Universidade do Oeste de Santa Catarina, campus de Joaçaba, e-mail: [email protected] (Autor para correspondência), [email protected]. 2. FAPESC - Fundação de Apoio e Amparo à Pesquisa Científica do Estado de Santa Catarina, e-mail: [email protected]. 3. UTFPR - Universidade Tecnológica Federal do Paraná, campus de Francisco Beltrão, e-mail: [email protected] _____________________________________ EntomoBrasilis 4(1): 10-12 (2011) Resumo. As bromélias são plantas de característica marcante devido a disposição de suas folhas permitindo o acúmulo de água entre as mesmas, criando verdadeiros ecossistemas sobre a planta e permitindo o abrigo e desenvolvimento de diversos invertebrados, uma diversidade que muitas vezes passa despercebida. O presente estudo teve como objetivo analisar a importância das bromélias como mantenedoras de biodiversidade no Parque Natural Municipal Rio do Peixe, localizado no município de Joaçaba, centro-oeste de Santa Catarina, sul do Brasil (27º10’22”S, 51º30’33”W). O Parque Natural está localizado em área de ecótono florestal, caracterizando-se por uma transição entre Floresta Estacional Semidecidual e Floresta Ombrófila Mista. O estudo foi conduzido durante o período de janeiro de 2009 a janeiro de 2010. Ao longo do período de amostragem foram encontrados aproximadamente 168 invertebrados nos fitotelmatos das 48 bromélias coletadas, dentre os quais as ordens mais abundantes foram Hymenoptera (46 exemplares) e Coleoptera (36 exemplares). -
Phytogeography and Vegetation of Tropical Inselbergs
PHYTOGEOGRAPHY AND VEGETATION OF TROPICAL INSELBERGS W. BARTHLOTI-, S. POREMBSKI,J. SZARZYNSKI,J. P. MUND Botanisches Institut der Universitat Bonn. Meckenheimer Allee 170, D-53115 Bonn, Germany Résumé : Un bref aperçu de la végétation des inselbergs néo-et paléotropicaux est présenté. Les données proviennent d'études effectuées sur le terrain en Afrique de l'Ouest (Côte-d'Ivoire, Guinée, Cameroun), en Afrique de l'Est (Malawi), à Madagascar, au Brésil, au Venezuela et en Guyane française. Les habitats rocheux isolés sont occupéspar une flore adaptée 8 ce milieu particulier qui diffère presque complètement de la végé- tation environnante. Ils constituent ainsi des modèles pour l'étude de I'écologie insulaire. Les surfaces de roche apparemment nues sont ordinairement couvertes de cyanobactéries (ex. Guyane française) ou de lichens (ex. Côte-d'Ivoire). La végétation discontinue d'Angiospermes est constituée d'une mosaïque de différentes formations. Les plus remarquables sont caractérisées par des tapis de Monocotylédones (Broméliacbesdans la région néotropicale et Cypéracées dans la région paléotropicale), des végétationsmarécageuses sur dallegranitique humide (plusieurs espèces d'Utricularia, deGenlisea, de Burmannia ainsi que d'Eriocaulacées)et des groupements saisonniers de plantes dansles cuvettes rocheuses (ex. Scrophulariacées). On trouve des types biologiques convergents dans les régions paléo- et néotropicales (ex. rosettes caulescentes chez les Cypéracées et Velloziacées). Mots-clés : Biodiversité,géobotanique, inselbergs, écologie insulaire, phytogéographie, rochers découverts. Brorné- liacées,Cactacées, Velloziacées. Abstract: A short survey of the vegetation of neo- and paleo-tropical rock outcrops (inselbergs) is provided. The data are based on field studies in West Africa (Ivory Coast, Guinea, Cameroon), East Africa (Malawi), Madagascar, Brazil, Venezuela and French Guyana. -
Far North Coast Bromeliad Study Group N.S.W
Far North Coast Bromeliad Study Group N.S.W. Study Group meets the third Thursday of each month Next meeting August 20th 2015 at 11 a.m. Venue: PineGrove Bromeliad Nursery 114 Pine Street Wardell 2477 Phone (02) 6683 4188 Discussion: July 2015 General Discussion Editorial Team: Kay Daniels Trish Kelly Ross Little Helen Clewett [email protected] Statements and opinions expressed in articles are those of the authors and are not necessarily endorsed by the Group. Articles appearing in this News Letter may be used in other Publications provided that the source is credited. 1 Meeting 18th June 2015 Some discussion was had about ‘Beginnner Classes’, by all means tell us what you want to know/do and we’ll make it happen for you, be it potting mixes, pup The meeting was opened at approximately 11.00 am removal, identifying the various parts of a flower or any other point of interest to The 18 members and one visitor present were welcomed. you about bromeliads. It is up to the longer serving more experienced growers A total of seven apologies were received. among us to pass on their knowledge to our beginners and you’ll be surprised what hints and tricks you’ll pick-up along the way yourself. There are members General Business at all levels in every Group more than willing to pass on info., so don’t be afraid to ask any questions and if we hear a “I’m not at liberty to say” well you guys Unfortunately our two regular scribes were unable to attend our July meeting so know my answer to that as The aim of our Group is: you have to put up with my ramblings this month. -
Bromelcairns
Club Activities & Around the Members SEPTEMBER: We met at Kewarra at Dave & Brendan’s where the trees & shrubs have all grown well and the bromeliads are thriving. Members wandered around Bromelcairns the garden and were amazed when they saw Dyckia ‘Betty Farnhill’ aka ‘BB’ in Newsletter - 2008 # 5 spike! ---- small plants had formed in the centre of the cluster of large plants Cairns Bromeliad Society Inc. and were in flower, the orange bells appeared to be laughing at us. It was an P.O.Box 28 Cairns. Queensland 4870 amazing sight! No, Dave did not de-pup it! * With 13 good neoregelias entered in the mini show it was hard to judge but President Bob Hudson 0740533913 V-President Thomas Jones unlisted Brendan’s Neoregelia ‘Leopard’ was just perfect, with Darryl’s ‘Fandango’ a close Secretary Lynn Hudson 0740533913 second and a spider with web relegated Dave’s very rich ‘Burgundy’ to third. Treasurer Sharron Miller 0740322283 Excellent miniatures. ‘Well Done’ to each Entrant. Librarian Stuart Howe 0402832354 * We had a short discussion on neoregelias, then on varied topics as questions Editor Lynn Hudson 0740533913 arose and then the inevitable ingredients/ratio for ‘pot mixes’. Editor Assist. * We discussed the Show schedule and agreed on changes that include the BSI OIC Raffles Karen Stevens 0740361086 Member Concierge Barry Osborne 0740532047 definition of ‘flowering’ and for some genera to be more than one specimen. The Popular Vote Steward Karen Cross 0740545497 Schedule will be distributed next February. *Honorary Life Member - Grace Goode O.A.M.* * As Sharron had kept requesting a bus trip Bob suggested we go to Butler’s Life Member - Lynn Hudson Open Garden & visit Greg Oldano. -
An Alphabetical List of Bromeliad Binomials
AN ALPHABETICAL LIST OF BROMELIAD BINOMIALS Compiled by HARRY E. LUTHER The Marie Selby Botanical Gardens Sarasota, Florida, USA ELEVENTH EDITION Published by the Bromeliad Society International June 2008 ii INTRODUCTION TO EDITION XI This list is presented as a spelling guide for validly published taxa accepted at the Bromeliad Identification Center. The list contains the following information: 1) Genus number (the left-hand number) based on the systematic sequence published in the Smith & Downs monograph: Bromeliaceae (Flora Neotropica, number 14, parts 1-3; 1974, 1977, 1979). Whole numbers are as published in the monograph. 2) Species number (the second number) according to its systematic position in the monograph. Note: Taxa not included in the monograph or that have been reclassified have been assigned numbers to reflect their systematic position within the Smith & Downs framework (e.g., taxon 14.1 is related to taxon 14). The utility of this method is that one may assume for example that Tillandsia comarapaensis (150.2) is related to T. didisticha (150) and therefore may have certain horticultural qualities in common with that species. 3) Genus and species names follow the respective numbers. 4) Subspecific taxa (subspecies, varieties, forms) names are indented below the species names. Note: Variety "a" (the type variety) is not listed unless it contains a form (see Aechmea caudata ). Similarly, the type form is not listed. 5) Author name follows the specific and subspecific names. These names are included for the convenience of specialist users of the list. This list does not contain publication data or synonymy, as it is not our intent for it to be a technical nomenclatural guide. -
Supplementary Material What Do Nectarivorous Bats Like? Nectar Composition in Bromeliaceae with Special Emphasis on Bat-Pollinated Species
Supplementary Material What do nectarivorous bats like? Nectar composition in Bromeliaceae with special emphasis on bat-pollinated species Author: Thomas Göttlinger, Michael Schwerdtfeger, Kira Tiedge, Gertrud Lohaus* *Correspondence: Gertrud Lohaus ([email protected]) Supplementary Figure S1: Concentration of sugars (glucose, fructose, sucrose) in nectar of seven genera of Bromeliaceae (Alcantarea (A), Guzmania (B), Pitcairnia (C), Puya (D), Tillandsia (E), Vriesea (F), Werauhia (G)) which include bat-pollinated species. The box plots show medians (horizontal line in box) and means (x in box). Supplementary Material What do nectarivorous bats like? Nectar composition in Bromeliaceae with special emphasis on bat-pollinated species Author: Thomas Göttlinger, Michael Schwerdtfeger, Kira Tiedge, Gertrud Lohaus* *Correspondence: Gertrud Lohaus ([email protected]) Supplementary Figure S2: Concentration of amino acids (ala, arg, asn, asp, gaba, gln, glu, gly, his, iso, leu, lys, met, phe, pro, ser, thr, trp, tyr, val) in nectar of seven genera of Bromeliaceae (Alcantarea (A), Guzmania (B), Pitcairnia (C), Puya (D), Tillandsia (E), Vriesea (F), Werauhia (G)), which include bat-pollinated species. The box plots show medians (horizontal line in box) and means (x in box). Supplementary Material What do nectarivorous bats like? Nectar composition in Bromeliaceae with special emphasis on bat-pollinated species Author: Thomas Göttlinger, Michael Schwerdtfeger, Kira Tiedge, Gertrud Lohaus* *Correspondence: Gertrud Lohaus ([email protected]) Supplementary Figure S3: Cation concentrations (Ca2+, K+, Na+, Mg2+) in nectar of seven genera of Bromeliaceae (Alcantarea (A), Guzmania (B), Pitcairnia (C), Puya (D), Tillandsia (E), Vriesea (F), Werauhia (G)), which include bat-pollinated species. The box plots show medians (horizontal line in box) and means (x in box). -
2Do(). '!Phe . Famuy . Are Generally Con§Picu Mono
Rev. Bio\. Trop., 46(3):493-513, 1998 Current. floristk and phytogeographk knowledge of Mexican Bromeliaceae Adolfo Espejo Serna yAna Rosa López-Ferrari1 I Herbario Metropolitano, Depart¡unento de Biología,C.B.S., Universidad Autónoma Metropolitana, Unidad Jztapalapa, Apartado Postal 55,535,09340 México, D. F.,Fax 7244688, e-m<'lil: [email protected] Rece.ived 6-XI-1997. Corrected 28-V-1998. Accepted 19-VI-1998. Abstract: A current floristicand phytogeographic knowledge of native Mexican Bromeliaceae is presented. There are 22 genera of Brorlleliaceae recorded from the country Iha! ¡nelude 326 species. The genus Ursulaea with 2 species is endemic to Mexico, wbíle Hechtiawith 48 oC its 50 specíesbas its principal centerof diversity in the country. 7illandsia (175 spp), Hechtia (48 spp) and Pitcairnia (46 spp) are tbe genera with tbe greatest number of species. We present a comparative análysisof Mexican Bromeliaceae with tbat of other American regions that buve recently published accounts Cor the Family, .particularlythe Mesomerican area,Venezu¡:la, Ecuador, and tbeGuianas.Our results ledus to the cOI1e1usiontbat alltbese floras sbould be considered as distinct. We obse,rve a progressive decre¡¡¡se ofthe Simpson index value related wit� tbe remoteness of the Mexican area. A general análysisof tlrpspeCies numbers of Mexican bromeliad genera shows adistinct preference oftbespeci es forconiferousand oakfo,rests'; folÍowed by t�opical caduci ' folious forests. There is also significan! r¡:presentation of tbe family ifi'o ther vegetation types such as doud forests and tropical perennifolious forests. Generally Mel\ican Bromeliacea¡: speeies hav¡: scárceand sparse populationsandin manyc ases they inbabit diffs,bluffs and scaIJÍs in restrlcted areas,Col1cerning tbe.