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Conference Committees Executive Committee Prof. Dr. Ghulamqadir Kazi Prof. Dr. Mehmet Karatas Prof. Dr. Umar Ali Khan Dr. Muhammad Aasim Dr. Zahid Iqbal Organizing Committee Prof. Dr. Umar Ali Khan Prof. Dr. lshtiaq Ahmed Dr. Zahid Iqbal Prof. Dr. Ghulam Mustafa Lodhi Mr. Mustafa Minhas Mr. Aafaq Ahmed Technical Committee Prof. Dr. Mehmet Karatas - Turkey Prof. Dr. Raheel Qamar - Pakistan Prof. Dr. lshtiaq Ahmed - Pakistan Prof. Dr. Aamir Ali Khan - Pakistan Prof. Dr. ljaz Javed Hasan - Pakistan Prof. Dr. Zafar Iqbal- Pakistan Dr. Muhammad Aasim - Turkey Dr. Munir Ahmad-Pakistan Dr. Muhammad Asif Aziz - Pakistan Dr. Awais Rasheed-China Dr. Riaz Hussain - Pakistan Dr. Muhammad Aamer Mehmood, Pakistan Dr. Naeem Asghar - Pakistan Dr. Faqir Muhammad- USA Dr. Khalid F. Salamat - UK Dr. Mazhar ul Haq - Pakistan Dr. Mansur Abdullah - Pakistan Dr. Muhammad Kashif Saleemi - Pakistan Dr. Zahid Iqbal-Pakistan Designing & Publication Mr. Muhammad Imran Mr. Muhammad Laiq Khan Asian J Agri Biol, Special Issue-2015 ANTIFUNGAL POTENTIAL OF PLANT EXTRACTS FOR THE MANAGEMENT OF ANTHRACNOSE OF CHILI CAUSED BY COLLETOTRICHUM CAPSICI Muhammad Nasir Subhani1*, Muhammad Bilal Chattha1 and Waseem Abbas2 1Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan 2Arid Zone Research Institute, Bhakar, Pakistan ABSTRACT Chili anthracnose damages chili fruits extensively at pre- and postharvest stages causing anthracnose lesions. Even very small lesion of anthracnose on fruits of chili reduces the market value of chili crop. Fungitoxic effects of methanolic plant extracts were tested in vitrothrough poisoned food technique. There was a significant decrease in mycellial growth of the fungus with an increase in methanolic plant extracts concentration in all the tested methanolic plant extracts over the control. The fungitoxicity of methanolic plant extracts varied greatly among each other and their concentrations. In general, there was a decrease in mycelial growth Colletotrichum capsici with an increase in concentration of methanolic plant extracts. However, when growth of the fungus in response to various methanolic plant extract concentrations after an incubation period of nine days at 28 ± 2°C was compared, neem leaf extract proved to be the best as it had given the maximum control (64 percent) followed by datura leaf extract (63 percent) on 1000 µg/ml concentration. While eucalyptus leaf extract was proved to be the least effective (45 percent) at highest concentration (1000 µg/ml). None of the tested methanolic plant extracts has completely checked the mycelial growth of Colletotrichum capsici at any of their concentrations. Although there was an overall trend of reduction in mycelial growth of Colletotrichum capsiciwith increase in the concentration of methanollic plant extracts.These plant extracts can be used to controlanthracnose of chilies to reduce the use of fungicides, as plant extracts are safer than synthetic fungicides. Keywords: methanolic plant extracts, Chili, anthracnose, Colletotrichum capsici *Correspondence author email: A NOVEL TOOL FOR EFFICIENT AND RAPID EXTRACTION OF PHENOLICS FROM BARK OF CASSIA OCCIDENTALIS L. Sumera Javada*, Shabnam Shaheena, Farah Khanb, Sidra Younasc a Assistant professor, b Professor, c Researcher Dept. of Botany LCWU, Lahore ABSTRACT Microwave assisted extraction is a modern and new approach for rapid and efficient extraction of plant material which are rich in number of secondary metabolites of prime importance. In present study a comparison was made between conventional method of extraction i.e., Soxhlet extraction, and modern method of extraction i.e., Microwave assisted extraction for the rapid and efficient extraction of Cassia occidentalis Linn bark. Microwave assisted extraction was proved to be more rapid and more efficient for the extraction of bark of Cassia occidentalis which yielded 0.19mg of extract/g of bark powder in 70 sec of microwave irradiation of 700W as compared to 0.16mg of extract/g of bark powder in 14 hours of continuous heating of the soxhlet extraction. It was also observed that a maximum of 89 µg equivalent of Gallic acid of phenolics were obtained from extract of Microwave heating (900W) for 30 seconds. While 45 µg equivalent of Catechin of flavonoids were obtained from a microwave assisted extract at 60 seconds of 700W of irradiation. In contrast, soxhlet extraction produced only 47.6 equivalent of gallic acid and 27.3 µg equivalent of Catechin after 12 hours of extraction. So it was proved that microwave assisted extraction was efficient as well as cost effective in terms of lesser use of energy, time and solvent. Keywords: *Correspondence author email: CLSR-2015 Abstracts 1 Asian J Agri Biol, Special Issue-2015 ANTIBIOTIC RESISTANCE OF BACTERIAL BIOFILMS Amna Butt Fatima Jinnah Women University, the mall, Rawalpindi ABSTRACT Biofilms are microorganisms’ communities that get attached to a surface. It is evident through various researches that cells grown in a biofilm express distinct properties from that of planktonic cells, one of such property which is of high significance is an increased resistance to antimicrobial/ antibiotic agents. Bacterial biofilms are a cause of chronic infections worldwide because of their increased antibiotic tolerance. Their resistance to the antibiotics is due to mutation, resistant phenotypes, adaptations to stress, quorum sensing, stratified activity, nutrient gradients, oxidative stress, failure of antibiotic penetration and heterogeneity. Thus in order to control the bacterial infections caused by these biofilms, there is a need of novel drug delivery approaches and enhanced therapeutic use of quorum sensing inhibitors. Keywords: Biofilms, antibiotic resistance, quorum sensing, quorum sensing inhibitors *Correspondence author email: [email protected] BIOENERYGY A STEP TOWARDS SUSTAINABILITY Mehwish Jadoon Fatima Jinnah Women University. The Mall, Rawalpindi. ABSTRACT Extraction of energy from the biological sources is the most environmental friendly way to conserve the natural resources. The biomass used for the production of energy comprises of the crops (corn, sugarcane and many others) and other way is the waste (e.g., wastewater from food industries, wood waste, straw, manure, sludge from sewerage etc). Bio energy extraction tends to shift from nonrenewable resources to the renewable resources of energy Microbe’s serves as directly in the extraction of bio energy as the algae the best source of bio fuel, and indirectly microbes are taking part in decomposition process for the formation of biogas. Geo bacter species are the naturally electric current generating species and playing an important role in the microbial fuel cell technology. Bio gas and bio diesel are the cheapest ones and least pollution generating as well as neutral to CO2 emissions. Rather it is appropriate to say it is the greener technology and a step towards sustainability. Bio gas plants are installed in Pakistan for extraction of bio gas. This extraction is limited only to the research level and there is not any use of algal bio fuel. Pakistan is rich in the resources for the production of bio energy but unfortunately lack of technologies and financial assistance is the main hindrance behind its implication. Bio energy is increasing in response to concerns about energy security, energy independence and environmental and climate impacts associated with use of non- renewable energy resources. Keywords: Bio energy, Bio fuel, Environmental friendly, Greener technology. *Correspondence author email: [email protected] CLSR-2015 Abstracts 2 Asian J Agri Biol, Special Issue-2015 POOR SOLID WASTE MANAGEMENT AND PROBLEMS RELATED TO WASTE COLLECTORS IN PAKISTAN Nusrat Shaheen1, Muhammad Anwar Baig2, Imran Hashmi2 Dr. Ghulam Akbar3, Farhat Jabeen1, Javeria Abbas1 and Muhammad Qasim1 1Masters Student, 2 IESE, National University of Science and Technology, Islamabad, (Pakistan) 3 WWF-Pakistan, Islamabad, (Pakistan) ABSTRACT The solid waste management is really the critical issue in Pakistan and trash pickers are in very crucial conditions. They are facing the condition of low status, very miserable living conditions, and poverty. All of them are surrounded by large number of problems from risks in domestic waste to the chemical waste which they collect on daily basis. Number of waste collectors is increasing day by day because the poor people who are illiterate have no any other option to earn for their families. So all family members get their self involved in this profession which does not require any education or any experience. Most of them are not aware of safe work practices, which causes large number of work hazards.Pakistan has very poor occupational safety and health laws and implementation is very low. Keywords: Waste Collectors, Occupational Health & Safety, Solid Waste Management *Correspondence author email: PROSTATE CANCER AND GLUTATHIONE S TRANSFERASE DELETIONS Saima Shakil Malik, Nosheen Masood, Azra Yasmin Fatima Jinnah Women University, Rawalpindi, Pakistan ABSTRACT GSTM1 and GSTT1 gene polymorphisms have been studied in many populations to evaluate their association with prostate cancer risk with contrasting results. The current study was aimed to find out the association of GSTM1 and GSTT1 gene polymorphisms with prostate cancer in Pakistani men. This case control study included pathologically confirmed prostate cancer patients and age matched male controls. Epidemiological data was collected by a standard questionnaire and
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