Revista Mexicana de Ingeniería Química ISSN: 1665-2738 [email protected] Universidad Autónoma Metropolitana Unidad Iztapalapa México Vázquez-Silva, G.; Castro-Mejía J, J.; Sánchez de la Concha, B.; González-Vázquez, R.; Mayorga-Reyes, L.; Azaola-Espinosa, A. BIOENCAPSULATION OF Bifidobacterium animalis AND Lactobacillus johnsonii IN Artemia franciscana AS FEED FOR CHARAL (Chirostoma jordani) LARVAE Revista Mexicana de Ingeniería Química, vol. 15, núm. 3, 2016, pp. 809-818 Universidad Autónoma Metropolitana Unidad Iztapalapa Distrito Federal, México Available in: http://www.redalyc.org/articulo.oa?id=62048168012 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Vol. 15, No. 3 (2016) 809-818 Revista Mexicana de Ingeniería Química Ingeniería de alimentos BIOENCAPSULATION OF BifidobacteriumCONTENIDO animalis AND Lactobacillus johnsonii IN Artemia franciscana AS FEED FOR CHARAL (Chirostoma jordani) LARVAE Volumen 8, número 3, 2009 / Volume 8, number 3, 2009 BIOENCAPSULACI ON´ DE Bifidobacterium animalis Y Lactobacillus johnsonii EN Artemia franciscana COMO ALIMENTO DE LARVAS DE CHARAL (Chirostoma jordani) G. Vazquez-Silva´ 2131, Derivation J. Castro-Mej and application´ıa J1, B. of Sanchez´ the Stefan-Maxwell de la Concha equations2, R. Gonzalez-V´ azquez´ 2, L. Mayorga-Reyes2, A. Azaola-Espinosa2* 1Departamento El Hombre(Desarrollo y suAmbiente. y aplicación2 Departamentode las ecuaciones Sistemas de Stefan-Maxwell) Biol´ogicos.Universidad Aut´onomaMetropolitana, Unidad Stephen WhitakerXochimilco. Calz del Hueso 1100, Coyoac´an04960. CDMX Received June 2, 2016; Accepted August 1, 2016 Biotecnología / Biotechnology Abstract An experiment245 was Modelado designed de to evaluatela biodegradación the filling en and biorreactores effect of probiotic de lodos bacteria de hidrocarburos in metanauplii totales of delArtemia petróleoas a vehicle and feed source for Chirostoma jordani, an endemic fish species with socio-economic importance and as a protein source intemperizados en suelos y sedimentos for the population from the central region of Mexico.´ The optimum filling of metanauplii of Artemia with Lactobacillus 3 −1 johnsonii and Bifidobacterium (Biodegradation animalis modelingwas of achieved sludge bioreactors in 40 min of with total a petroleum concentration hydrocarbons of 2:0 × weathering10 CFU mLin soil of viable cells of L. johnsoniiandand sediments)B. animalis , respectively, being enough to fill up in 40 min the metanauplii of Artemia with 628 cells of B. animalis and 688 cells of L. johnsonii. During the experiment, 20 metanauplii with or without probiotic bacteria S.A. Medina-Moreno, S. Huerta-Ochoa, C.A. Lucho-Constantino, L. Aguilera-Vázquez, A. Jiménez- were consumed per fish per day. The encapsulated probiotics promoted the growth and increased the weight and specific growth rate of the larvaeGonzález of C.y M. jordani Gutiérrez-Rojascompared with the control (P < 0.05). L. johnsonii had a better probiotic effect on metanauplii of C.259 jordani Crecimiento,(P < 0.05). sobrevivencia y adaptación de Bifidobacterium infantis a condiciones ácidas Keywords: bacterial supplements, enriched live food, Artemia, charal, Chirostoma jordani, growth indicators. (Growth, survival and adaptation of Bifidobacterium infantis to acidic conditions) Resumen L. Mayorga-Reyes, P. Bustamante-Camilo, A. Gutiérrez-Nava, E. Barranco-Florido y A. Azaola- Se disen˜o´ un experimento para evaluar la capacidad de ingestion´ de Artemia y el efecto de dos bacterias probioticas´ Espinosa encapsuladas en metanauplios de Artemia como veh´ıculo y fuente de alimento para larvas de charal Chirostoma jordani, especie endemica´ 265 de Statistical importancia approach socioecon to optimizationomica´ y, fuente of ethanol de prote fermentation´ınas y de recursosby Saccharomyces economicos´ cerevisiae de lospobladores in the de la region´ central de Mpresenceexico.´ La of capacidadValfor® zeolite de llenado NaA en metanauplios de Artemia se evaluo´ en 40 min, en agua enriquecida con celulas´ viables de Lactobacillus johnsonii y Bifidobacterium animalis en una concentracion´ de 2:0 × 103 CFU ml−1. Durante el experimento (Optimización de alimentaci estadísticaon´ de de larvas, la fermentación 20 metanauplii etanólica de deArtemia Saccharomycescon o sin cerevisiae bacterias en fueron presencia consumidos de por pez por d´ıa. Los probizeolitaoticos´ Valfor® bioencapsulados zeolite NaA) en Artemia favorecieron el incremento en peso y talla as´ı como tasa espec´ıfica de crecimiento en larvasG. Inei-Shizukawa, de C. jordani H., enA. comparaciVelasco-Bedrán,on´ al grupoG. F. Gutiérrez-López control (P <0,05). and H.Artemia Hernández-Sánchezenriquecida con L. johnsonii, ademas´ mejoro´ significativamente la supervivencia larvas de C. jordani (P <0,05). Palabras clave: bacterias beneficas,´ alimento vivo enriquecido, Artemia, charal, Chirostoma jordani, indicadores de crecimiento. Ingeniería de procesos / Process engineering 271 Localización de una planta industrial: Revisión crítica y adecuación de los criterios empleados en esta decisión (Plant site selection: Critical review and adequation criteria used in this decision) 1 IntroductionJ.R. Medina, R.L. Romero y G.A. Pérez In the production of fish, metanauplii of Artemia content (57%) and fatty acids, which are essential for are considered the best food for the diet of aquatic the nutrition of fish and crustaceans (Malpica et al., organisms in their early larval stages. Artemia is 2004; Luna et al., 2009). considered the most widely used food as feed in the larval stages or larval production due to its Other attributes is the adaptability of Artemia nutritional value and easy digestion (Tonheim et al., to several culture media, easy handling and ability 2000). Regarding its nutritional composition, Artemia to incorporate materials such as nutrients, pigments, is considered a feed of high quality due to its protein emulsions (Kyungmin et al., 2000), antibiotics (Rodr´ıguez et al., 2011), microalgae, and bacteria * Corresponding author. E-mail: [email protected] Tel/Fax. 248-48-154-82 Publicado por la Academia Mexicana de Investigacion´ y Docencia en Ingenier´ıa Qu´ımica A.C. 809 V´azquez-Silvaet al./ Revista Mexicana deIngenier ´ıaQu ´ımica Vol. 15, No. 3 (2016) 809-818 (Subhadra et al., 2010) into its body. In aquaculture, that improve digestibility, feed efficiency, performance bioencapsulation is a technique of enrichment of live and growth (Wang et al., 2008). The addition of prey widely used, which includes improvement living probiotics in diets has been suggested as an alternative feed through the incorporation of microorganisms or to improve the survival, welfare and growth of substances inside rotifers, Cladocerans or Artemia, fish, including larval stages (Ringø and Gatesoupe which function at the same time as vectors or carriers 1998; Gomez-Gil´ et al., 1998; Verschuere et al., of such components facilitating the administration of 2000; Wang et al., 2008). Furthermore, produce its content to host (Saarela et al., 2000; Dey et al., organic acids as metabolic products that inhibit the 2015). Artemia can undergo encapsulation, which growth of pathogenic bacteria, and also antibacterial facilitates the administration of different products or substances such as bacteriocins. Lactic acid bacteria microorganisms because it acts as a carrier or live are a common bacterial probiotic for animal and capsule, allowing products to reach consumers directly fish production. In the case of Bifidobacteria, there while avoiding deterioration by water (Verschuere et is not enough information about its effect on fish al., 2000; Gelabert, 2003). production; there has only been found information Probiotics have been widely used at the larval regarding trout (Kopecn˘ y´ et al., 2010). Lactic acid stages to control bacterial infections and the presence bacteria and Bifidobacteria have α-and β-glucosidase, of potential pathogens instead of antibiotics in β-galactosidase, β-fructofuranosidase and phytase subtherapeutic doses (Monroy-Dosta et al., 2010); activities, among others, that help with good intestinal however, the use of antibiotics has increased the colonization (Hayek et al., 2013, Pokusaeva et al., tolerance and virulence of the bacteria and generated 2011). For good intestinal colonization, these bacteria significant economic losses (Verschuere et al., 2000) also show good tolerance to acidic conditions and as well as making practices more environmentally bile acids. Some of these properties of probiotics friendly (Taoka et al., 2006). This fact has have been shown in a few species of fish, mainly favored the use of probiotics, which in adequate species such as trout (Araujo´ et al., 2015), but not concentrations, confer health benefits to the host in small animals by its reduced size. All of these (Irianto & Austin, 2002), whilst addressing global properties are critical for conferring health benefits on demands to reduce the use of antibiotics in animal the host (Ren et al., 2014). The addition of probiotics production. According to Ziaei-Nejad et al., (2006) in feed has shown a significant increase in the yield metanauplii of Artemia have been used as vehicles of freshwater species such as goldfish (Ahilan et al., for the transport of probiotics in aquatic animals, 2004) and swordfish green swordtail with