DOCSLIB.ORG

Plenary Lecture & Symposium

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Plenary Lecture & Symposium PLENARY LECTURE & SYMPOSIUM SYMPOSIuM From genomics to flagellar and ciliary struc - MONDAY 29 JulY tures and cytoskeleton dynamics (by FEPS) PlENARY lECTuRE (ISoP Honorary Member lECTuRE) Chairs (by ISoP) Cristina Miceli , University of Camerino, Camerino, Italy Helena Soares , University of Lisbon and Gulbenkian Foun - Introduction - John Dolan , CNRS-Sorbonne University, Ville - dation, Lisbon, Portugal franche-sur-Mer, France. Jack Sunter - Oxford Brookes University, Oxford, UK- Genome Tom Fenchel University of Copenhagen, Copenhagen, Den - wide tagging in trypanosomes uncovers flagellum asymmetries mark Dorota Wloga - Nencki Institute of Experimental Biology, War - ISoP Honorary Member saw, Poland - Deciphering the molecular mechanisms that coor - dinate ciliary outer doublet complexes – search for “missing Size, Shape and Function among Protozoa links” Helena Soares - University of Lisbon and Polytechnic Institute of Lisbon, Lisbon, Portugal - From centrosomal microtubule an - SYMPOSIuM on ciliate biology and taxonomy in memory choring and organization to basal body positioning: TBCCD1 an of Denis lynn (by FEPS/ISoP) elusive protein Chairs Pierangelo luporini , University of Camerino, Camerino, Italy Roberto Docampo , University of Georgia, Athens, Georgia TuESDAY 30 JulY Alan Warren - Natural History Museum, London, UK. The bio - logy and systematics of peritrich ciliates: old concepts and new PlENARY lECTuRE (PAST-PRESIDENT LECTURE, by ISoP) findings Rebecca Zufall - University of Houston, Houston, USA. Amitosis Introduction - Avelina Espinosa , Roger Williams University, and the Evolution of Asexuality in Tetrahymena Ciliates Bristol, USA Sabine Agatha - University of Salzburg, Salzburg, Austria. The biology and systematics of oligotrichean ciliates: new findings David Bass and old concepts Natural History Museum London, London & Cefas, Weymouth, laura utz - School of Sciences, PUCRS, Porto Alegre, Brazil. Ci - Dorset, UK liate diversity and ecological interactions in Neotropical envi - ronments Micro-eukaryotes in animal and plant microbiomes: ecologies of disease? SYMPOSIuM Protist diversity and function in the dark ocean - challenging the paradigms of deep-sea ecology (by SYMPOSIuM ISOP ADVANCES Ten years of metabarcoding: FEPS) what have we learned and how do we move forward? (by ISoP/ B&R Moore Foundation) Chairs John Dolan, CNRS-Sorbonne University, Villefranche-sur- Chairs Mer, France, luciana Santoferrara University of Connecticut, Storrs, Hartmut Arndt , University of Cologne, Cologne, Germany USA. George McManus ,University of Connecticut, Storrs, USA John Dolan - CNRS-Sorbonne University, Villefranche-sur-mer, France. - Protistan Microzooplankton in the Mesopelagic Medi - luciana Santoferrara - University of Connecticut, Storrs, USA. terranean Sea Strengths and limitations of metabarcoding for assessing aquatic Andrew J. Gooday - National Oceanography Centre, Southam - protist communities pton - Biodiversity and biogeography of deep-sea benthic fora - Fabien Burki - Uppsala University, Uppsala, Sweden. Long me - minifera and their possible roles in bathyal and abyssal tabarcoding of the eukaryotic rDNA operon to phylogenetically ecosystems and taxonomically resolve environmental diversity Hartmut Arndt , University of Cologne, Cologne, Germany - Fla - Ramiro logares - Institute of Marine Sciences, Barcelona, gellates, ciliates and amoebae may occupy all niches of deep-sea Spain. Hypothesis testing and inference of ecological patterns microbial life from large marine metabarcoding datasets Alexandra Schoenle , University of Cologne, Cologne, Ger - Sabine Filke r - University of Kaiserslautern, Kaiserslautern, many- Global distribution and unique protist communities in the Germany. Metabarcoding as a basis to study the diversity and deep sea ecophysiology of halophilic microeukaryotes 1 VIII ECOP-ISOP JOINT CONGRESS 2019 SYMPOSIuM All Roads lead to Rome: Comparing Molecu - Adaptation Strategies of the Invasive Bloom-Forming Dinofla - lar and Cellular Paths to Eukaryotic Multicellularity gellates in Brackish Waters (by ISoP) SYMPOSIuM Free-living amoeba and neglected pathogen Chairs protozoa: health emergency signals? (by FEPS) Michelle leger CSIC -University Pompeu Fabra, Barcelona, Spain Chairs Sebastián Najie , CSIC -University Pompeu Fabra, Barce - Joel Dacks , University of Alberta, Alberta, Canada. lona, Spain Maria Cristina Angelici , National Institute of Health, Rome, Italy Pauline Schaap - University of Dundee, Dundee, UK. Evolution of multicellularity in the Amoebozoan lineage Joel Dacks - University of Alberta, Alberta, Canada. A compa - Alexander Tice - Mississippi State University, Starkville, USA. rative ‘omics approach to pathogenicity in the Brain-eating Comparing paths to multicellularity in amoeboid protists amoeba, Naegleria fowleri Thibaut Brunet - University of California, Berkeley, USA. In - Julia Walochnik - University of Vienna, Vienna, Austria. Patho - sights into the origin of animal contractile cells from choanofla - genicity in Acanthamoeba gellates Patrick Scheid - Bundeswehr Central Hospital in Koblenz, Ko - lászló Nagy - Hungarian Academy of Sciences, Budapest, Hun - blenz, Germany. “Free living amoebae as pathogens and as vec - gary. Not all follow the same road: fungi took a unique evolutio - tors of endocytobionts”. nary path to multicellularity Adriana Calderaro - University of Parma, Parma, Italy. Intesti - nal protozoa searching for a disease or for a doctor? SYMPOSIuM ISOP ADVANCES Applications of Genetic Tools for Advancing Research on Marine Protists (by ISoP/B&R SYMPOSIuM Host-Parasite Interactions in Vector-borne Moore Foundation) Protozoan Infections (by FEPS) Chairs Chairs Virginia Edgcomb , Woods Hole Oceanographic Institute, Marina Gramiccia National Institute of Health, Rome, Italy Woods Hole, USA, luigi Gradoni National Institute of Health, Rome, Italy Julius lukes , Czech Academy of Sciences, České Budějovice, Czech Republic Gad Baneth - Hebrew University, Rehovot, Israel. Babesia spe - cies of Domestic Dogs – Where do they Fit in the Piroplasmid Binnypreet Kaur - Czech Academy of Sciences, České Budějo - World? vice, Czech Republic. Diplonema papillatum, a representative of Paul Bates - Lancaster University, Lancaster, UK. Revising Lei - the highly diverse and abundant marine microeukaryotes, can shmania’s life cycle in the phlebotomine vector. be genetically manipulated Anna Olivieri - National Institute of Health, Rome, Italy. Pla - Ross Waller - Cambridge University, Cambridge, UK. Transfor - smodium development in the mosquito midgut. A molecular and ming Dinozoa: steps forward and steps back”. cellular view. Fatma Gomaa - Harvard University, Cambridge, USA and Woods Hole Oceanographic Institution, Woods Hole, USA. Tools for stable integrative transfection of Bodo saltans: A micro-eu - karyote with polycistronic peptide coding genes SYMPOSIuM Waterborne infections of protozoan origin: Jackie Collier - Stony Brook University, NY, USA. Successful ge - how much do we really know? (by FEPS) netic manipulation of the thraustochytrid Aurantiochytrium li - macinum and challenges extending to other labyrinthulomycetes Chairs Cristina Miceli - University of Camerino, Camerino, Italy. Tran - Panagiotis Karanis , University of Cologne, Cologne, Ger - sfection and reverse genetics in marine ciliates with highly am - many plified nanochromosomes Maria Cristina Angelici , National Institute of Health, Rome, Italy Panagiotis Karanis - University of Cologne, Cologne, Germany. Wednesday 31 July Waterborne Protozoan Infections: Health Problem Dimension Worldwide PlENARY lECTuRE (by FEPS) Melissa Palos ladeiro - University of Reims Champagne-Ar - Introduction - Maria Cristina Angelici, National Institute of denne, Reims, France. Mollusc Bivalves as Indicators of conta - Health, Rome, Italy mination of water bodies by protozoan parasites Judit Plutzer - National Public Health Institute, Budapest, Hun - Sergey Skarlato gary. New directions in parasite detection: micro-and macroflui - Russian Academy of Sciences, St. Petersburg, Russia dic platforms 2 PLENARY LECTURE & SYMPOSIUM Maria Cristina Angelici - National Institute for Health, Rome, Diane Stoecker - University-Md-Horn Point Lab, Cambridge, Italy. Waterbone Protozoan Infections in the Climatic Changes USA. Mixotrophs - food webs and harmful algal blooms Era. George McManus - University of Connecticut, Storrs, USA. Mi - xotrophs and challenges for experimental studies Fabrice Not –CNRS-Sorbonne University, Roscoff, France. Mi - Thursday 01 August xotrophs and challenges for molecular biology Aditee Mitra - Swansea University, Swansea, UK Challenges for PlENARY lECTuRE (by FEPS) modelling the perfect beast Introduction - Giulio Petroni , University of Pisa, Pisa, Italy SYMPOSIuM Bioactive molecules from protists: perspecti - Sergey Fokin ves in Biotechnology (by FEPS) University of Pisa, Pisa, Italy Chairs Prof. H.-D. Goertz and his contribution to our knowledge of pro - Adriana Vallesi and Sandra Pucciarelli tozoan symbiosis. University of Camerino, Camerino, Italy SYMPOSIuM Symbiosis in ciliates: H.-D. Görtz and his le - Marco Mangiagalli - University of Milan, Milan, Italy. Life gacy (by FEPS) under stress: ice binding proteins and superoxide dismutases from an Antarctic ciliate. Chairs Angelo Fontana - (ICB) CNR, Naple, Italy. Function and biote - Giulio Petroni , University of Pisa, Pisa, Italy chnological research of metabolites from marine protists Martina Schrallhammer , University of Freiburg, Freiburg, Federico
Load more

Recommended publications
  • Denis BAURAIN Département Des Sciences De La Vie Université De Liège Société Royale Des Sciences De Liège 20 Septembre 2012 Plan De L’Exposé
    L’évolution des Eucaryotes Denis BAURAIN Département des Sciences de la Vie Université de Liège Société Royale des Sciences de Liège 20 septembre 2012 Plan de l’exposé 1. Qu’est-ce qu’un Eucaryote ? 2. Quelle est la diversité des Eucaryotes ? 3. Quelles sont les relations de parenté entre les grands groupes d’Eucaryotes ? 4. D’où viennent les Eucaryotes ? Qu’est-ce1 qu’un Eucaryote ? Eukaryotic Cells définition ultrastructurale : organelles spécifiques • noyau (1) • nucléole (2) • RE (5, 8) • Golgi (6) • centriole(s) (13) • mitochondrie(s) (9) • chloroplaste(s) • ... http://en.wikipedia.org/ A eukaryotic gene is arranged in a patchwork of coding (exons) and non-coding sequences (introns). Introns are eliminated while exons are spliced together to yield the mature mRNA used for protein synthesis. http://reflexions.ulg.ac.be/ Gene DNA Transcription Exon1 Exon2 Exon3 Exon4 Exon5 Exon6 pre-mRNA Alternatif splicing mature mRNA Translation Protein In many Eukaryotes, almost all genes can lead to different proteins through a process termed alternative splicing. http://reflexions.ulg.ac.be/ REVIEWS Box 2 | Endosymbiotic evolution and the tree of genomes Intracellular endosymbionts that originally descended from free-living prokaryotes have been important in the evolution of eukaryotes by giving rise to two cytoplasmic organelles. Mitochondria arose from α-proteobacteria and chloroplasts arose from cyanobacteria. Both organelles have made substantial contributions to the complement of genes that are found in eukaryotic nuclei today. The figure shows a schematic diagram of the evolution of eukaryotes, highlighting the incorporation of mitochondria and chloroplasts into the eukaryotic lineage through endosymbiosis and the subsequent co-evolution of the nuclear and organelle genomes.
    [Show full text]
  • TEXT-BOOKS of ANIMAL BIOLOGY a General Zoology of The
    TEXT-BOOKS OF ANIMAL BIOLOGY * Edited by JULIAN S. HUXLEY, F.R.S. A General Zoology of the Invertebrates by G. S. Carter Vertebrate Zoology by G. R. de Beer Comparative Physiology by L. T. Hogben Animal Ecology by Challes Elton Life in Inland Waters by Kathleen Carpenter The Development of Sex in Vertebrates by F. W. Rogers Brambell * Edited by H. MUNRO Fox, F.R.S. Animal Evolution / by G. S. Carter Zoogeography of the Land and Inland Waters by L. F. de Beaufort Parasitism and Symbiosis by M. Caullery PARASITISM AND ~SYMBIOSIS BY MAURICE CAULLERY Translated by Averil M. Lysaght, M.Sc., Ph.D. SIDGWICK AND JACKSON LIMITED LONDON First Published 1952 !.lADE AND PRINTED IN GREAT BRITAIN BY WILLIAM CLOWES AND SONS, LlMITED, LONDON AND BECCLES CONTENTS LIST OF ILLUSTRATIONS vii PREFACE TO THE ENGLISH EDITION xi CHAPTER I Commensalism Introduction-commensalism in marine animals-fishes and sea anemones-associations on coral reefs-widespread nature of these relationships-hermit crabs and their associates CHAPTER II Commensalism in Terrestrial Animals Commensals of ants and termites-morphological modifications in symphiles-ants.and slavery-myrmecophilous plants . 16 CHAPTER III From Commensalism to Inquilinism and Parasitism Inquilinism-epizoites-intermittent parasites-general nature of modifications produced by parasitism 30 CHAPTER IV Adaptations to Parasitism in Annelids and Molluscs Polychates-molluscs; lamellibranchs; gastropods 40 CHAPTER V Adaptation to Parasitism in the Crustacea Isopoda-families of Epicarida-Rhizocephala-Ascothoracica
    [Show full text]
  • COEVOLUTIONARY RELATIONSHIPS in a NEW TRIPARTITE MARINE SYMBIOSIS Phylogenetic Affinities of the Symbiotic Protist Nephromyces
    COEVOLUTIONARY RELATIONSHIPS IN A NEW TRIPARTITE MARINE SYMBIOSIS Phylogenetic affinities of the symbiotic protist Nephromyces Mary Beth Saffo Marine Biological Laboratory, Woods Hole & Museum of Comparative Zoology, Harvard University Abstract 2. Phenotypic features suggestive of general protistan affinities All molgulid ascidian tunicates (Urochordata, phylum Chordata) : •tubular mitochondrial cristae (fig. 5,8) contain, in the lumen of the peculiar, ductless “renal sac,” an equally •thraustochytrid-like sporangia (fig. 4); other developmental stages bear vague peculiar symbiotic protist, Nephromyces. From the first description of resemblances to various other protistan taxa. Nephromyces in the 1870’s, the phylogenetic affinities, even existence, of this taxon has been a matter of debate. Though classified by Alfred Giard in 1888 as a chytridiomycete, Nephromyces has 3. Ssu rDNA sequences indicate that Nephromyces is an apicomplexan clade (preliminary phylogenetic analysis: a sample resisted definitive identification with any particular fungal or protistan Maximum likelihood trees shown) taxa. While its chitinous walls and hyphal-like cells support the notion of fungal affinities for Nephromyces, many other features , including the divergent phylogenetic implications of its morphologically eclectic life cycle , do not. Sequencing of ssu rDNA, indicates, however, that Nephromyces is an apicomplexan; these molecular data are supported by apicomplexan-like characters in the morphology Nephromyces’ host-transfer and infective stages. Other morphological and biochemical features of Nephromyces , some of Phylogenetic analyses, still in progress, consistently indicate thatNephromyces is a distinct them unusual among apicomplexa, or even protists generally, clade within the Apicomplexa. Although the challenge us to consider the significance of certain phenotypic relationship of Nephromyces to other apicomplexan characters as diagnostic tools in inferring deep phylogenies.
    [Show full text]
  • The Planktonic Protist Interactome: Where Do We Stand After a Century of Research?
    bioRxiv preprint doi: https://doi.org/10.1101/587352; this version posted May 2, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Bjorbækmo et al., 23.03.2019 – preprint copy - BioRxiv The planktonic protist interactome: where do we stand after a century of research? Marit F. Markussen Bjorbækmo1*, Andreas Evenstad1* and Line Lieblein Røsæg1*, Anders K. Krabberød1**, and Ramiro Logares2,1** 1 University of Oslo, Department of Biosciences, Section for Genetics and Evolutionary Biology (Evogene), Blindernv. 31, N- 0316 Oslo, Norway 2 Institut de Ciències del Mar (CSIC), Passeig Marítim de la Barceloneta, 37-49, ES-08003, Barcelona, Catalonia, Spain * The three authors contributed equally ** Corresponding authors: Ramiro Logares: Institute of Marine Sciences (ICM-CSIC), Passeig Marítim de la Barceloneta 37-49, 08003, Barcelona, Catalonia, Spain. Phone: 34-93-2309500; Fax: 34-93-2309555. [email protected] Anders K. Krabberød: University of Oslo, Department of Biosciences, Section for Genetics and Evolutionary Biology (Evogene), Blindernv. 31, N-0316 Oslo, Norway. Phone +47 22845986, Fax: +47 22854726. [email protected] Abstract Microbial interactions are crucial for Earth ecosystem function, yet our knowledge about them is limited and has so far mainly existed as scattered records. Here, we have surveyed the literature involving planktonic protist interactions and gathered the information in a manually curated Protist Interaction DAtabase (PIDA). In total, we have registered ~2,500 ecological interactions from ~500 publications, spanning the last 150 years.
    [Show full text]
  • Fungal Evolution: Major Ecological Adaptations and Evolutionary Transitions
    Biol. Rev. (2019), pp. 000–000. 1 doi: 10.1111/brv.12510 Fungal evolution: major ecological adaptations and evolutionary transitions Miguel A. Naranjo-Ortiz1 and Toni Gabaldon´ 1,2,3∗ 1Department of Genomics and Bioinformatics, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona 08003, Spain 2 Department of Experimental and Health Sciences, Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain 3ICREA, Pg. Lluís Companys 23, 08010 Barcelona, Spain ABSTRACT Fungi are a highly diverse group of heterotrophic eukaryotes characterized by the absence of phagotrophy and the presence of a chitinous cell wall. While unicellular fungi are far from rare, part of the evolutionary success of the group resides in their ability to grow indefinitely as a cylindrical multinucleated cell (hypha). Armed with these morphological traits and with an extremely high metabolical diversity, fungi have conquered numerous ecological niches and have shaped a whole world of interactions with other living organisms. Herein we survey the main evolutionary and ecological processes that have guided fungal diversity. We will first review the ecology and evolution of the zoosporic lineages and the process of terrestrialization, as one of the major evolutionary transitions in this kingdom. Several plausible scenarios have been proposed for fungal terrestralization and we here propose a new scenario, which considers icy environments as a transitory niche between water and emerged land. We then focus on exploring the main ecological relationships of Fungi with other organisms (other fungi, protozoans, animals and plants), as well as the origin of adaptations to certain specialized ecological niches within the group (lichens, black fungi and yeasts).
    [Show full text]
  • Filling Gaps in the Microsporidian Tree: Rdna Phylogeny of Chytridiopsis Typographi (Microsporidia: Chytridiopsida)
    Parasitology Research (2019) 118:169–180 https://doi.org/10.1007/s00436-018-6130-1 GENETICS, EVOLUTION, AND PHYLOGENY - ORIGINAL PAPER Filling gaps in the microsporidian tree: rDNA phylogeny of Chytridiopsis typographi (Microsporidia: Chytridiopsida) Daniele Corsaro1 & Claudia Wylezich2 & Danielle Venditti1 & Rolf Michel3 & Julia Walochnik4 & Rudolf Wegensteiner5 Received: 7 August 2018 /Accepted: 23 October 2018 /Published online: 12 November 2018 # Springer-Verlag GmbH Germany, part of Springer Nature 2018 Abstract Microsporidia are intracellular eukaryotic parasites of animals, characterized by unusual morphological and genetic features. They can be divided in three main groups, the classical microsporidians presenting all the features of the phylum and two putative primitive groups, the chytridiopsids and metchnikovellids. Microsporidia originated from microsporidia-like organisms belong- ing to a lineage of chytrid-like endoparasites basal or sister to the Fungi. Genetic and genomic data are available for all members, except chytridiopsids. Herein, we filled this gap by obtaining the rDNA sequence (SSU-ITS-partial LSU) of Chytridiopsis typographi (Chytridiopsida), a parasite of bark beetles. Our rDNA molecular phylogenies indicate that Chytridiopsis branches earlier than metchnikovellids, commonly thought ancestral, forming the more basal lineage of the Microsporidia. Furthermore, our structural analyses showed that only classical microsporidians present 16S-like SSU rRNA and 5.8S/LSU rRNA gene fusion, whereas the standard eukaryote rRNA gene structure, although slightly reduced, is still preserved in the primitive microsporidians, including 18S-like SSU rRNA with conserved core helices, and ITS2-like separating 5.8S from LSU. Overall, our results are consistent with the scenario of an evolution from microsporidia-like rozellids to microsporidians, however suggesting for metchnikovellids a derived position, probably related to marine transition and adaptation to hyperparasitism.
    [Show full text]
  • Divergence Time Estimates and the Evolution of Major Lineages in The
    www.nature.com/scientificreports OPEN Divergence time estimates and the evolution of major lineages in the florideophyte red algae Received: 31 March 2015 Eun Chan Yang1,2, Sung Min Boo3, Debashish Bhattacharya4, Gary W. Saunders5, Accepted: 19 January 2016 Andrew H. Knoll6, Suzanne Fredericq7, Louis Graf8 & Hwan Su Yoon8 Published: 19 February 2016 The Florideophyceae is the most abundant and taxonomically diverse class of red algae (Rhodophyta). However, many aspects of the systematics and divergence times of the group remain unresolved. Using a seven-gene concatenated dataset (nuclear EF2, LSU and SSU rRNAs, mitochondrial cox1, and plastid rbcL, psaA and psbA genes), we generated a robust phylogeny of red algae to provide an evolutionary timeline for florideophyte diversification. Our relaxed molecular clock analysis suggests that the Florideophyceae diverged approximately 943 (817–1,049) million years ago (Ma). The major divergences in this class involved the emergence of Hildenbrandiophycidae [ca. 781 (681–879) Ma], Nemaliophycidae [ca. 661 (597–736) Ma], Corallinophycidae [ca. 579 (543–617) Ma], and the split of Ahnfeltiophycidae and Rhodymeniophycidae [ca. 508 (442–580) Ma]. Within these clades, extant diversity reflects largely Phanerozoic diversification. Divergences within Florideophyceae were accompanied by evolutionary changes in the carposporophyte stage, leading to a successful strategy for maximizing spore production from each fertilization event. Our research provides robust estimates for the divergence times of major lineages within the Florideophyceae. This timeline was used to interpret the emergence of key morphological innovations that characterize these multicellular red algae. The Florideophyceae is the most taxon-rich red algal class, comprising 95% (6,752) of currently described species of Rhodophyta1 and possibly containing many more cryptic taxa2.
    [Show full text]
  • An Integrative Approach Sheds New Light Onto the Systematics
    www.nature.com/scientificreports OPEN An integrative approach sheds new light onto the systematics and ecology of the widespread ciliate genus Coleps (Ciliophora, Prostomatea) Thomas Pröschold1*, Daniel Rieser1, Tatyana Darienko2, Laura Nachbaur1, Barbara Kammerlander1, Kuimei Qian1,3, Gianna Pitsch4, Estelle Patricia Bruni4,5, Zhishuai Qu6, Dominik Forster6, Cecilia Rad‑Menendez7, Thomas Posch4, Thorsten Stoeck6 & Bettina Sonntag1 Species of the genus Coleps are one of the most common planktonic ciliates in lake ecosystems. The study aimed to identify the phenotypic plasticity and genetic variability of diferent Coleps isolates from various water bodies and from culture collections. We used an integrative approach to study the strains by (i) cultivation in a suitable culture medium, (ii) screening of the morphological variability including the presence/absence of algal endosymbionts of living cells by light microscopy, (iii) sequencing of the SSU and ITS rDNA including secondary structures, (iv) assessment of their seasonal and spatial occurrence in two lakes over a one‑year cycle both from morphospecies counts and high‑ throughput sequencing (HTS), and, (v) proof of the co‑occurrence of Coleps and their endosymbiotic algae from HTS‑based network analyses in the two lakes. The Coleps strains showed a high phenotypic plasticity and low genetic variability. The algal endosymbiont in all studied strains was Micractinium conductrix and the mutualistic relationship turned out as facultative. Coleps is common in both lakes over the whole year in diferent depths and HTS has revealed that only one genotype respectively one species, C. viridis, was present in both lakes despite the diferent lifestyles (mixotrophic with green algal endosymbionts or heterotrophic without algae).
    [Show full text]
  • A Fossilized Microcenosis in Triassic Amber
    J Eukaqlnr. Micmbrol., 46(6), 1999 pp. 571-584 0 1999 by the Society of Protozoologists A Fossilized Microcenosis in Triassic Amber WILFRIED SCHONBORN,a HEINRICH DORFELT? WILHELM FOISSNER,’ LOTHAR KRIENITZd and URSULA SCHAFER” “Friedrich-Schiller-UniversitatJena, Institut fur Okologie, Arbeitsgruppe Limnologie, Winzerlaer StraJe 10, 0-0774.5 Jena, Germany, and bFriedrich-Schiller-Univer.sitatJenn,lnstitut fiir Ernahrung und Umwelt, Lehrgebiet Lnndschaftsokologie und Naturschutz, DornburgerslraJe 159, 0-07743 Jena, Germany, and ‘Universitut Salzburg, Institut fur Zoologie, HellbrunnerstraJe 34, A-5020 Salzburg, Austria, and dInstitut fur Gewasseriikologie und Binnenjscherei, Alte Fischerhiitte 2, 0-16775 Neuglobsow, Germany ABSTRACT. Detailed data on bacterial and protistan microfossils are presented from a 0.003 mm3 piece of Triassic amber (Schlier- seerit, Upper Triassic period, 220-230 million years old). This microcenosis, which actually existed as such within a very small, probably semiaquatic habitat, included the remains of about two bacteria species, four fungi (Palaeodikaryomyces baueri, Pithomyces-like conidia, capillitium-like hyphae, yeast cells) two euglenoids, two chlamydomonas (Chlamydomonas sp., Chloromonas sp.), two coccal green microalgae (Chlorellu sp., Chorzcystis-like cells), one zooflagellate, three testate amoebae (Centropyxis aculeata var. oblonga-like, Cyclopyxis eurystoma-like, Hyalosphenia baueri n. sp.), seven ciliates (Pseudoplatyophrya nana-like, Mykophagophrys rerricola-like, Cvrtolophosis mucicola-like, Paracondylostoma
    [Show full text]
  • Raphidiophrys Contractilis
    Kobe University Repository : Thesis Mechanism of β-1,3-glucan mediated food uptake in the protozoon 学位論文題目 Raphidiophrys contractilis(原生生物Raphidiophrys contractilis におけ Title るβ-1, 3-グルカンが介在する捕食機構) 氏名 MOUSUMI BHADRA Author 専攻分野 博士（理学） Degree 学位授与の日付 2017-09-25 Date of Degree 公開日 2018-09-25 Date of Publication 資源タイプ Thesis or Dissertation / 学位論文 Resource Type 報告番号 甲第7000号 Report Number 権利 Rights JaLCDOI URL http://www.lib.kobe-u.ac.jp/handle_kernel/D1007000 ※当コンテンツは神戸大学の学術成果です。無断複製・不正使用等を禁じます。著作権法で認められている範囲内で、適切にご利用ください。 PDF issue: 2021-10-07 Doctoral Dissertation Mechanism of β-1, 3-glucan mediated food uptake in the protozoon Raphidiophrys contractilis 原生生物 Raphidiophrys contractilis における β-1, 3-グルカンが介在する捕食機構 July 2017 Graduate School of Science Kobe University Mousumi Bhadra Contents Acknowledgements ............................................................................................................ 2 Summary ........................................................................................................................... 3 Chapter 1: Introductory review............................................................................................ 7 Chapter 2: Proteins required for food capturing in Raphidiophrys contractilis..................... 11 2.1. Introduction ....................................................................................................... 11 2.2. Materials and methods ........................................................................................ 15 2.3. Results ..............................................................................................................
    [Show full text]
  • Real-Time Dynamics of Plasmodium NDC80 Reveals Unusual Modes of Chromosome Segregation During Parasite Proliferation Mohammad Zeeshan1,*, Rajan Pandey1,*, David J
    © 2020. Published by The Company of Biologists Ltd | Journal of Cell Science (2021) 134, jcs245753. doi:10.1242/jcs.245753 RESEARCH ARTICLE SPECIAL ISSUE: CELL BIOLOGY OF HOST–PATHOGEN INTERACTIONS Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation Mohammad Zeeshan1,*, Rajan Pandey1,*, David J. P. Ferguson2,3, Eelco C. Tromer4, Robert Markus1, Steven Abel5, Declan Brady1, Emilie Daniel1, Rebecca Limenitakis6, Andrew R. Bottrill7, Karine G. Le Roch5, Anthony A. Holder8, Ross F. Waller4, David S. Guttery9 and Rita Tewari1,‡ ABSTRACT eukaryotic organisms to proliferate, propagate and survive. During Eukaryotic cell proliferation requires chromosome replication and these processes, microtubular spindles form to facilitate an equal precise segregation to ensure daughter cells have identical genomic segregation of duplicated chromosomes to the spindle poles. copies. Species of the genus Plasmodium, the causative agents of Chromosome attachment to spindle microtubules (MTs) is malaria, display remarkable aspects of nuclear division throughout their mediated by kinetochores, which are large multiprotein complexes life cycle to meet some peculiar and unique challenges to DNA assembled on centromeres located at the constriction point of sister replication and chromosome segregation. The parasite undergoes chromatids (Cheeseman, 2014; McKinley and Cheeseman, 2016; atypical endomitosis and endoreduplication with an intact nuclear Musacchio and Desai, 2017; Vader and Musacchio, 2017). Each membrane and intranuclear mitotic spindle. To understand these diverse sister chromatid has its own kinetochore, oriented to facilitate modes of Plasmodium cell division, we have studied the behaviour movement to opposite poles of the spindle apparatus. During and composition of the outer kinetochore NDC80 complex, a key part of anaphase, the spindle elongates and the sister chromatids separate, the mitotic apparatus that attaches the centromere of chromosomes to resulting in segregation of the two genomes during telophase.
    [Show full text]
  • Protist Phylogeny and the High-Level Classification of Protozoa
    Europ. J. Protistol. 39, 338–348 (2003) © Urban & Fischer Verlag http://www.urbanfischer.de/journals/ejp Protist phylogeny and the high-level classification of Protozoa Thomas Cavalier-Smith Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK; E-mail: [email protected] Received 1 September 2003; 29 September 2003. Accepted: 29 September 2003 Protist large-scale phylogeny is briefly reviewed and a revised higher classification of the kingdom Pro- tozoa into 11 phyla presented. Complementary gene fusions reveal a fundamental bifurcation among eu- karyotes between two major clades: the ancestrally uniciliate (often unicentriolar) unikonts and the an- cestrally biciliate bikonts, which undergo ciliary transformation by converting a younger anterior cilium into a dissimilar older posterior cilium. Unikonts comprise the ancestrally unikont protozoan phylum Amoebozoa and the opisthokonts (kingdom Animalia, phylum Choanozoa, their sisters or ancestors; and kingdom Fungi). They share a derived triple-gene fusion, absent from bikonts. Bikonts contrastingly share a derived gene fusion between dihydrofolate reductase and thymidylate synthase and include plants and all other protists, comprising the protozoan infrakingdoms Rhizaria [phyla Cercozoa and Re- taria (Radiozoa, Foraminifera)] and Excavata (phyla Loukozoa, Metamonada, Euglenozoa, Percolozoa), plus the kingdom Plantae [Viridaeplantae, Rhodophyta (sisters); Glaucophyta], the chromalveolate clade, and the protozoan phylum Apusozoa (Thecomonadea, Diphylleida). Chromalveolates comprise kingdom Chromista (Cryptista, Heterokonta, Haptophyta) and the protozoan infrakingdom Alveolata [phyla Cilio- phora and Miozoa (= Protalveolata, Dinozoa, Apicomplexa)], which diverged from a common ancestor that enslaved a red alga and evolved novel plastid protein-targeting machinery via the host rough ER and the enslaved algal plasma membrane (periplastid membrane).
    [Show full text]