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PLENARY LECTURE & SYMPOSIUM SYMPOSIuM From genomics to flagellar and ciliary struc - MONDAY 29 JulY tures and cytoskeleton dynamics (by FEPS) PlENARY lECTuRE (ISoP Honorary Member lECTuRE) Chairs (by ISoP) Cristina Miceli , University of Camerino, Camerino, Italy Helena Soares , University of Lisbon and Gulbenkian Foun - Introduction - John Dolan , CNRS-Sorbonne University, Ville - dation, Lisbon, Portugal franche-sur-Mer, France. Jack Sunter - Oxford Brookes University, Oxford, UK- Genome Tom Fenchel University of Copenhagen, Copenhagen, Den - wide tagging in trypanosomes uncovers flagellum asymmetries mark Dorota Wloga - Nencki Institute of Experimental Biology, War - ISoP Honorary Member saw, Poland - Deciphering the molecular mechanisms that coor - dinate ciliary outer doublet complexes – search for “missing Size, Shape and Function among Protozoa links” Helena Soares - University of Lisbon and Polytechnic Institute of Lisbon, Lisbon, Portugal - From centrosomal microtubule an - SYMPOSIuM on ciliate biology and taxonomy in memory choring and organization to basal body positioning: TBCCD1 an of Denis lynn (by FEPS/ISoP) elusive protein Chairs Pierangelo luporini , University of Camerino, Camerino, Italy Roberto Docampo , University of Georgia, Athens, Georgia TuESDAY 30 JulY Alan Warren - Natural History Museum, London, UK. The bio - logy and systematics of peritrich ciliates: old concepts and new PlENARY lECTuRE (PAST-PRESIDENT LECTURE, by ISoP) findings Rebecca Zufall - University of Houston, Houston, USA. Amitosis Introduction - Avelina Espinosa , Roger Williams University, and the Evolution of Asexuality in Tetrahymena Ciliates Bristol, USA Sabine Agatha - University of Salzburg, Salzburg, Austria. The biology and systematics of oligotrichean ciliates: new findings David Bass and old concepts Natural History Museum London, London & Cefas, Weymouth, laura utz - School of Sciences, PUCRS, Porto Alegre, Brazil. Ci - Dorset, UK liate diversity and ecological interactions in Neotropical envi - ronments Micro-eukaryotes in animal and plant microbiomes: ecologies of disease? SYMPOSIuM Protist diversity and function in the dark ocean - challenging the paradigms of deep-sea ecology (by SYMPOSIuM ISOP ADVANCES Ten years of metabarcoding: FEPS) what have we learned and how do we move forward? (by ISoP/ B&R Moore Foundation) Chairs John Dolan, CNRS-Sorbonne University, Villefranche-sur- Chairs Mer, France, luciana Santoferrara University of Connecticut, Storrs, Hartmut Arndt , University of Cologne, Cologne, Germany USA. George McManus ,University of Connecticut, Storrs, USA John Dolan - CNRS-Sorbonne University, Villefranche-sur-mer, France. - Protistan Microzooplankton in the Mesopelagic Medi - luciana Santoferrara - University of Connecticut, Storrs, USA. terranean Sea Strengths and limitations of metabarcoding for assessing aquatic Andrew J. Gooday - National Oceanography Centre, Southam - protist communities pton - Biodiversity and biogeography of deep-sea benthic fora - Fabien Burki - Uppsala University, Uppsala, Sweden. Long me - minifera and their possible roles in bathyal and abyssal tabarcoding of the eukaryotic rDNA operon to phylogenetically ecosystems and taxonomically resolve environmental diversity Hartmut Arndt , University of Cologne, Cologne, Germany - Fla - Ramiro logares - Institute of Marine Sciences, Barcelona, gellates, ciliates and amoebae may occupy all niches of deep-sea Spain. Hypothesis testing and inference of ecological patterns microbial life from large marine metabarcoding datasets Alexandra Schoenle , University of Cologne, Cologne, Ger - Sabine Filke r - University of Kaiserslautern, Kaiserslautern, many- Global distribution and unique protist communities in the Germany. Metabarcoding as a basis to study the diversity and deep sea ecophysiology of halophilic microeukaryotes 1 VIII ECOP-ISOP JOINT CONGRESS 2019 SYMPOSIuM All Roads lead to Rome: Comparing Molecu - Adaptation Strategies of the Invasive Bloom-Forming Dinofla - lar and Cellular Paths to Eukaryotic Multicellularity gellates in Brackish Waters (by ISoP) SYMPOSIuM Free-living amoeba and neglected pathogen Chairs protozoa: health emergency signals? (by FEPS) Michelle leger CSIC -University Pompeu Fabra, Barcelona, Spain Chairs Sebastián Najie , CSIC -University Pompeu Fabra, Barce - Joel Dacks , University of Alberta, Alberta, Canada. lona, Spain Maria Cristina Angelici , National Institute of Health, Rome, Italy Pauline Schaap - University of Dundee, Dundee, UK. Evolution of multicellularity in the Amoebozoan lineage Joel Dacks - University of Alberta, Alberta, Canada. A compa - Alexander Tice - Mississippi State University, Starkville, USA. rative ‘omics approach to pathogenicity in the Brain-eating Comparing paths to multicellularity in amoeboid protists amoeba, Naegleria fowleri Thibaut Brunet - University of California, Berkeley, USA. In - Julia Walochnik - University of Vienna, Vienna, Austria. Patho - sights into the origin of animal contractile cells from choanofla - genicity in Acanthamoeba gellates Patrick Scheid - Bundeswehr Central Hospital in Koblenz, Ko - lászló Nagy - Hungarian Academy of Sciences, Budapest, Hun - blenz, Germany. “Free living amoebae as pathogens and as vec - gary. Not all follow the same road: fungi took a unique evolutio - tors of endocytobionts”. nary path to multicellularity Adriana Calderaro - University of Parma, Parma, Italy. Intesti - nal protozoa searching for a disease or for a doctor? SYMPOSIuM ISOP ADVANCES Applications of Genetic Tools for Advancing Research on Marine Protists (by ISoP/B&R SYMPOSIuM Host-Parasite Interactions in Vector-borne Moore Foundation) Protozoan Infections (by FEPS) Chairs Chairs Virginia Edgcomb , Woods Hole Oceanographic Institute, Marina Gramiccia National Institute of Health, Rome, Italy Woods Hole, USA, luigi Gradoni National Institute of Health, Rome, Italy Julius lukes , Czech Academy of Sciences, České Budějovice, Czech Republic Gad Baneth - Hebrew University, Rehovot, Israel. Babesia spe - cies of Domestic Dogs – Where do they Fit in the Piroplasmid Binnypreet Kaur - Czech Academy of Sciences, České Budějo - World? vice, Czech Republic. Diplonema papillatum, a representative of Paul Bates - Lancaster University, Lancaster, UK. Revising Lei - the highly diverse and abundant marine microeukaryotes, can shmania’s life cycle in the phlebotomine vector. be genetically manipulated Anna Olivieri - National Institute of Health, Rome, Italy. Pla - Ross Waller - Cambridge University, Cambridge, UK. Transfor - smodium development in the mosquito midgut. A molecular and ming Dinozoa: steps forward and steps back”. cellular view. Fatma Gomaa - Harvard University, Cambridge, USA and Woods Hole Oceanographic Institution, Woods Hole, USA. Tools for stable integrative transfection of Bodo saltans: A micro-eu - karyote with polycistronic peptide coding genes SYMPOSIuM Waterborne infections of protozoan origin: Jackie Collier - Stony Brook University, NY, USA. Successful ge - how much do we really know? (by FEPS) netic manipulation of the thraustochytrid Aurantiochytrium li - macinum and challenges extending to other labyrinthulomycetes Chairs Cristina Miceli - University of Camerino, Camerino, Italy. Tran - Panagiotis Karanis , University of Cologne, Cologne, Ger - sfection and reverse genetics in marine ciliates with highly am - many plified nanochromosomes Maria Cristina Angelici , National Institute of Health, Rome, Italy Panagiotis Karanis - University of Cologne, Cologne, Germany. Wednesday 31 July Waterborne Protozoan Infections: Health Problem Dimension Worldwide PlENARY lECTuRE (by FEPS) Melissa Palos ladeiro - University of Reims Champagne-Ar - Introduction - Maria Cristina Angelici, National Institute of denne, Reims, France. Mollusc Bivalves as Indicators of conta - Health, Rome, Italy mination of water bodies by protozoan parasites Judit Plutzer - National Public Health Institute, Budapest, Hun - Sergey Skarlato gary. New directions in parasite detection: micro-and macroflui - Russian Academy of Sciences, St. Petersburg, Russia dic platforms 2 PLENARY LECTURE & SYMPOSIUM Maria Cristina Angelici - National Institute for Health, Rome, Diane Stoecker - University-Md-Horn Point Lab, Cambridge, Italy. Waterbone Protozoan Infections in the Climatic Changes USA. Mixotrophs - food webs and harmful algal blooms Era. George McManus - University of Connecticut, Storrs, USA. Mi - xotrophs and challenges for experimental studies Fabrice Not –CNRS-Sorbonne University, Roscoff, France. Mi - Thursday 01 August xotrophs and challenges for molecular biology Aditee Mitra - Swansea University, Swansea, UK Challenges for PlENARY lECTuRE (by FEPS) modelling the perfect beast Introduction - Giulio Petroni , University of Pisa, Pisa, Italy SYMPOSIuM Bioactive molecules from protists: perspecti - Sergey Fokin ves in Biotechnology (by FEPS) University of Pisa, Pisa, Italy Chairs Prof. H.-D. Goertz and his contribution to our knowledge of pro - Adriana Vallesi and Sandra Pucciarelli tozoan symbiosis. University of Camerino, Camerino, Italy SYMPOSIuM Symbiosis in ciliates: H.-D. Görtz and his le - Marco Mangiagalli - University of Milan, Milan, Italy. Life gacy (by FEPS) under stress: ice binding proteins and superoxide dismutases from an Antarctic ciliate. Chairs Angelo Fontana - (ICB) CNR, Naple, Italy. Function and biote - Giulio Petroni , University of Pisa, Pisa, Italy chnological research of metabolites from marine protists Martina Schrallhammer , University of Freiburg, Freiburg, Federico
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  • Real-Time Dynamics of Plasmodium NDC80 Reveals Unusual Modes of Chromosome Segregation During Parasite Proliferation Mohammad Zeeshan1,*, Rajan Pandey1,*, David J

    Real-Time Dynamics of Plasmodium NDC80 Reveals Unusual Modes of Chromosome Segregation During Parasite Proliferation Mohammad Zeeshan1,*, Rajan Pandey1,*, David J

    © 2020. Published by The Company of Biologists Ltd | Journal of Cell Science (2021) 134, jcs245753. doi:10.1242/jcs.245753 RESEARCH ARTICLE SPECIAL ISSUE: CELL BIOLOGY OF HOST–PATHOGEN INTERACTIONS Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation Mohammad Zeeshan1,*, Rajan Pandey1,*, David J. P. Ferguson2,3, Eelco C. Tromer4, Robert Markus1, Steven Abel5, Declan Brady1, Emilie Daniel1, Rebecca Limenitakis6, Andrew R. Bottrill7, Karine G. Le Roch5, Anthony A. Holder8, Ross F. Waller4, David S. Guttery9 and Rita Tewari1,‡ ABSTRACT eukaryotic organisms to proliferate, propagate and survive. During Eukaryotic cell proliferation requires chromosome replication and these processes, microtubular spindles form to facilitate an equal precise segregation to ensure daughter cells have identical genomic segregation of duplicated chromosomes to the spindle poles. copies. Species of the genus Plasmodium, the causative agents of Chromosome attachment to spindle microtubules (MTs) is malaria, display remarkable aspects of nuclear division throughout their mediated by kinetochores, which are large multiprotein complexes life cycle to meet some peculiar and unique challenges to DNA assembled on centromeres located at the constriction point of sister replication and chromosome segregation. The parasite undergoes chromatids (Cheeseman, 2014; McKinley and Cheeseman, 2016; atypical endomitosis and endoreduplication with an intact nuclear Musacchio and Desai, 2017; Vader and Musacchio, 2017). Each membrane and intranuclear mitotic spindle. To understand these diverse sister chromatid has its own kinetochore, oriented to facilitate modes of Plasmodium cell division, we have studied the behaviour movement to opposite poles of the spindle apparatus. During and composition of the outer kinetochore NDC80 complex, a key part of anaphase, the spindle elongates and the sister chromatids separate, the mitotic apparatus that attaches the centromere of chromosomes to resulting in segregation of the two genomes during telophase.
  • Protist Phylogeny and the High-Level Classification of Protozoa

    Protist Phylogeny and the High-Level Classification of Protozoa

    Europ. J. Protistol. 39, 338–348 (2003) © Urban & Fischer Verlag http://www.urbanfischer.de/journals/ejp Protist phylogeny and the high-level classification of Protozoa Thomas Cavalier-Smith Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK; E-mail: [email protected] Received 1 September 2003; 29 September 2003. Accepted: 29 September 2003 Protist large-scale phylogeny is briefly reviewed and a revised higher classification of the kingdom Pro- tozoa into 11 phyla presented. Complementary gene fusions reveal a fundamental bifurcation among eu- karyotes between two major clades: the ancestrally uniciliate (often unicentriolar) unikonts and the an- cestrally biciliate bikonts, which undergo ciliary transformation by converting a younger anterior cilium into a dissimilar older posterior cilium. Unikonts comprise the ancestrally unikont protozoan phylum Amoebozoa and the opisthokonts (kingdom Animalia, phylum Choanozoa, their sisters or ancestors; and kingdom Fungi). They share a derived triple-gene fusion, absent from bikonts. Bikonts contrastingly share a derived gene fusion between dihydrofolate reductase and thymidylate synthase and include plants and all other protists, comprising the protozoan infrakingdoms Rhizaria [phyla Cercozoa and Re- taria (Radiozoa, Foraminifera)] and Excavata (phyla Loukozoa, Metamonada, Euglenozoa, Percolozoa), plus the kingdom Plantae [Viridaeplantae, Rhodophyta (sisters); Glaucophyta], the chromalveolate clade, and the protozoan phylum Apusozoa (Thecomonadea, Diphylleida). Chromalveolates comprise kingdom Chromista (Cryptista, Heterokonta, Haptophyta) and the protozoan infrakingdom Alveolata [phyla Cilio- phora and Miozoa (= Protalveolata, Dinozoa, Apicomplexa)], which diverged from a common ancestor that enslaved a red alga and evolved novel plastid protein-targeting machinery via the host rough ER and the enslaved algal plasma membrane (periplastid membrane).