NAAC SSR Cycle IV (2015-2020)
1.1. CURRICULUM DESIGN AND 1.1.1. CURRICULUM DEVELOPMENT AND DEVELOPMENT IMPLEMENTATION
SYLLABUS
THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN (AUTONOMOUS), SIVAKASI – 626 123. (Affiliated to Madurai Kamaraj University, Re-accredited with A Grade by NAAC, College with Potential for Excellence by UGC and Mentor Institution under UGC PARAMARSH)
DEPARTMENT OF BOTANY SYLLABUS 2017 AND LATER
S.No. Programme Name Page No. B.Sc. Botany with Specialization in Plant 1. 1 Biotechnology
M.Sc. Botany with Specialization in Plant 2. 88 Biotechnology
Page 1 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN (AUTONOMOUS), SIVAKASI – 626 123 . (Affiliated to Madurai Kamaraj University, Re-accredited with A Grade by NAAC and College with Potential for Excellence by UGC)
DEPARTMENT OF BOTANY B.Sc. DEGREE PROGRAMME IN BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY GUIDELINES FOR OUTCOME BASED EDUCATION WITH CHOICE BASED CREDIT SYSTEM (For those admitted in June 2017 and later) I. A. PREAMBLE The institutional vision is to emerge as a premier institution offering need-based, value conscious and career-oriented quality education to empower rural women with communicative competency and employment potential. With the advent of Autonomy in the year 2005, Choice Based Credit System (CBCS) is followed and it offers much flexibility to innovate and design the contents of each programme and align it with the institutional mission. Quality assurance developments in higher education have encouraged us to move towards outcomes-based approach to teaching, learning and assessment. Programme specifications define the students in terms of what they can do at the end of a programme or a particular level of study. This is a change from the more traditional approach where teachers tended to define courses in terms of what is taught, rather than what the student can do at the end of the course or programme. More directed and coherent curriculum, “more relevant” Graduates to industry and other stakeholders and Continuous Quality Improvement (CQI) are the benefits of OBE. A student-centered paradigm in higher education entails a shift from a more input- oriented curricular design based on the description of course content, to outcomes-based education in which the course content is developed in terms of learning outcomes. The implementation of Outcome Based Education with CBCS as per the UGC guidelines from the academic year 2019-2020 will definitely mark a paradigm shift from traditional education. B. OUTCOME BASED EDUCATION (OBE) FRAMEWORK
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Page 2 of 159 C. PROGRAMME EDUCATIONAL OBJECTIVES, PROGRAMME OUTCOMES AND PROGRAMME SPECIFIC OUTCOMES Programme Educational Objectives (PEOs): PEOs are broad statements that describe the career and professional achievements that the programme is preparing the graduates to achieve within the first few years after graduation. PEOs should be consistent with the mission of the Institution. PEO’s can be measured by a PO-PEO matrix. The PEO’s should evolve through constant feedback from alumnae, students, industry, management etc,. It is mandatory that each PEO should be mapped to atleast one of the POs. The Graduates will PEO1 : be a productive employee in herbal industries, botanical gardens, educational institutions or pursue higher studies. PEO2 : create novel ideas to solve economic, social and environmental issues related to Botany. PEO3 : be competent to handle a demanding situation and involve in the collection and preparation of specimens to aspire as a successful entrepreneur. PEO4 : adhere to the principles of ethics in the production and usage of organic food materials both in professional and personal life. Programme Outcomes (PO): Programme Outcomes are narrower statements that describe what students are expected to know and be able to do upon the graduation. These relate to the skills, knowledge and behaviour that students acquire in their study through the programmes. PO1: Disciplinary knowledge Apply the knowledge of Arts, Science and Humanities to address fundamental and complex questions appropriate to their programmes. PO2: Critical thinking, Problem solving and Analytical reasoning Make use of appropriate knowledge and skills to identify, formulate, analyze and solve problems in order to reach substantiated conclusions. PO3: Research related skills and scientific reasoning Critically analyze research processes, products and practices with a view of strategic use of data in their field. PO4: Communication skills and Digital literacy Demonstrate skills in oral and written communication and make use of ICT in various learning ambience. PO5: Team work and Leadership quality Interact productively with people from diverse backgrounds as both leaders/mentors and team members with integrity and professionalism. PO6: Multicultural competence with Moral and ethical awareness Defend the society against gender and environmental issues with moral and ethical awareness.
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Page 3 of 159 PO7: Self-directed and Life-long learning Formulate their own educational needs in a changing world in ways sufficient to maintain their competence and to allow them to contribute to the advancement of knowledge. Programme Specific Outcomes (PSO): Programme Specific Outcomes denote what the students should be able to do at the time of graduation. They are programme specific. It is mandatory that each PO should be mapped to the respective PSO specified in the programme in order. By the completion of the B.Sc. Botany with specialization in Plant Biotechnology programme, the learners will be able to PSO1: apply their botanical knowledge to identify and compare the fundamental features and processes of different groups of plants. PSO2: critically think and apply the understanding of the subject of plant sciences in identifying the problems which can be solved through the use of plants. PSO3: evaluate and apply the skills in Botany with the understanding of general laboratory practices. PSO4: articulate effectively with the use of digital resources to explain the concepts related to life sciences. PSO5: exhibit their caliber to work efficiently as a member /leader in teams, preferably in a multi-disciplinary setting. PSO6: reflect the environmental values and uses of plants and be aware of environmental implications. PSO7: engage in sustainable utilization of plants, land, water, forest and energy resources. BLOOM’S TAXONOMY: Bloom's Taxonomy was created in 1956 by an educational psychologist Dr. Benjamin Bloom in order to promote higher forms of thinking in education, such as analyzing and evaluating concepts, processes, procedures, and principles, rather than just remembering facts. It is most often used when designing educational, training, and learning processes.
The K-levels mentioned in the diagram are usually denoted as [k1] to [k6] respectively from the bottom.
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Page 4 of 159 Course Outcomes (CO):
Course Outcomes are narrower statements that describe what students are expected to know and be able to do at the end of each course. These relate to the skills, knowledge, and behaviour that students acquire in their study through the course. Each course comprises five COs and the keywords used to define COs are based on Bloom’s Taxonomy[k1] to [k6]. On successful completion of the course, the learners should be able to
CO1: [k1] / [k2] CO2: [k3] CO3: [k4] CO4: [k5] CO5: [k6]
D. CO-PO & PO-PEO relationship:
E. CO – PO MAPPING OF COURSES: After CO statements are developed by the course in-charge, COs will be mapped with any possible POs based on the relationship exist between them. A CO must be mapped to atleast one PO. The PO’s which are not related to any of the COs in a particular course may be left blank. All the courses together must cover all the POs. The CO-PO matrix for a course is as shown below. The correlation between COs and PO can be defined by three levels using the Letter Grades H, M, L which denotes respectively High (H), Medium (M), Low (L) and ‘- ‘for no correlation. The concept of Six Sigma is used for calculating weighted percentage of contribution of each course in attainment of respective POs. As per Six Sigma Tool- Cause and Effect Matrix, the weightage of H, M and L are 9, 3 and 1 respectively.
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Page 5 of 159 CO-PO Mapping table (Course Articulation Matrix)
POs PO1 PO2 PO3 PO4 PO5 PO6 PO7
COs CO1 CO2 CO3 CO4 CO5 Weightage of the course Weighted percentage of Course contribution to POs
The levels of contribution are denoted by Grades and weightages H-High (9), M- Medium (3), L-Low (1)
Weighted percentage of Contribution of the Course in attainment of PO1= Weightage of the course / Total weightage of all courses contributing PO1 computed based on correlation between COs and POs X 100 Programme Articulation Matrix (PAM):
Course Course Title PO1 PO2 PO3 PO4 PO5 PO6 PO7 Code
Total Weightage of all courses contributing to POs
PO-PEO Mapping Matrix:
PEOs PEO1 PEO2 PEO3 PEO4 POs PO1 X X X PO2 X X PO3 X X PO4 X X PO5 X X X PO6 X X PO7 X (Mark X to map a PO to a PEO)
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Page 6 of 159 F. MEASUREMENT OF ATTAINMENT OF DESIRED GOALS: Course Outcome (CO) is measured through the performance of students by the various assessment tools adopted for that particular course. Each evaluation tool is mapped to a particular verb in Bloom’s Taxonomy and further each verb is mapped to a particular CO. Once the Course Outcome is measured, the PO can be measured using a CO-PO matrix. Measurement of PO attainment shall be done by direct and indirect methods. Direct assessment method and indirect assessment method are considered for 80% and 20% weightages respectively. Target levels of attainment shall be fixed by the Course teacher and Heads of the respective departments. Direct assessments (rubric based) - Conventional assessment tools such as Term Test, Quiz, Seminar, Assignment and End Semester Examination. Indirect assessments – Course Survey, Graduate Exit Survey, Feedback from Alumnae, Employer and Parents. G. ASSESSMENT PROCESS:
Assessment is one or more processes carried out by the institution that identify, collect and prepare data to evaluate the achievement of course outcomes and programme outcomes. Assessment Process for CO Attainment: For the evaluation and assessment of CO’s and PO’s, rubrics are used. (i) CO Assessment Rubrics: Course Outcome is evaluated based on the performance of students in the Continuous Internal Assessments and in End Semester Examination of a course. Internal assessment contributes 25% and End Semester assessment contributes 75% to the total attainment of a CO.
(ii) CO Assessment Tools: The description of Assessment tools used for the evaluation of COs and POs is given below. Mode of Assessment Description Evaluation of Course Related POs Assessment Tool Outcomes Theory Courses - Internal Assessment (Weightage 25%) Theory - Three written The questions in the three Term Test examinations Term Tests, Quiz and are conducted Assignment are framed in and average such a way that they cover of best two is all the COs of respective considered course. PO1 to PO7
Assignment One The final attainment for Assignment is each CO under direct Direct given per assessment is calculated (Weightage course by taking average of the 80%) Quiz/Seminar One CO attainments from Term Quiz/Seminar Tests, Assignment and
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Page 7 of 159 is conducted Quiz. for each course Theory Courses - External Assessment(Weightage 75%) End Semester Examination It covers the entire PO1 to PO7 Examination at the end of syllabus of the course. It the course of would generally satisfy all 3- hour course outcomes for a duration particular course. The COs are evaluated based on the set attainment levels.
Practical Courses - Internal Assessment (Weightage 40%) Observation Day to day Lab exercises are planned PO1 to PO7 evaluation to cover all COs and CO Model Model Exam attainment is calculated. Practical is conducted Examination for each lab course. Practical Courses - External Assessment (Weightage 60%) End Semester Examination The final attainment for PO1 to PO7 Practical at the end of each CO under direct Examination the course of assessment is calculated 3- hour by taking average of the duration CO attainments in Observation, Model Practicals and End Semester Practical Examination Indirect Course This survey At the end of each PO1 to PO7 (Weightage Survey gives the course an exit survey is 20%) opinion of the collected from the students on students and Considered attainment of for the CO attainment Course under Indirect assessment Outcomes
(iii) CO Attainment: Direct CO Attainment: Course outcomes of all courses are assessed and the CO wise marks obtained by all the students are recorded for all the assessment tools mentioned above. The respective CO attainment level is evaluated based on set attainment rubrics. Attainment Levels of COs
Assessment Methods Attainment Levels Level 1 60% of students scoring more than average marks or set target marks in internal assessment tools Level 2 70% of students scoring more than average marks or set target marks in internal assessment tools
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Page 8 of 159 Internal Assessment Level 3 75% of students scoring more than average marks or set target marks in internal assessment tools Level 1 60% of students scoring more than average marks or set target marks in End Semester Examination Level 2 70% of students scoring more than average marks End Semester Examination or set target marks in End Semester Examination Level 3 75% of students scoring more than average marks or set target marks in End Semester Examination
Target setting for Assessment method: For setting up the target of internal assessment tools, the average of last three tests must be taken into consideration and it should be kept as target. If the average marks are not available then current average can also be used as target. For setting up the target of End Semester Examination, the average of the last year examination shall be set as target. If the average marks are not available then current average can also be used as target. Formula for Attainment for each CO: Attainment = Percentage of students w ho have scored more than the target marks
Internal Attainment is the average of attainments oobtbtainedained using various internal assessment tools For Theory Courses, Direct CO Attainment =25% of internal attainment + 75%75% of End Semester attainment For Practical Courses, Direct CO Attainment =40% of internal attainment + 60%60% of End Semester attainment Indirect CO Attainment: At the end of each course, an exit survey is collected from the students and it gives the opinion of the students on attainment of Course Outcomes. A questionnaire is designed to reflect the views of the students about the attainment of course outcomes. Overall CO Attainment = 80% of Direct CO Attainment + 20% of Indirect CO Attainment In each course, the level of attainment of each CO is compared with the predefined targets, if the target is not reached, the course teacher takes necessary steps for the improvement to reach the target. If the average attainment of a particular course for two consecutive years is greater than 80% of th e maximum attainment value (i.e. 80% of 3 = 2.4), tthehenn for that particular course the current rubrics for attainment must be changed to analyze continuous improvement.
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Page 9 of 159 II. ASSESSMENT PROCESS FOR OVERALL PO ATTAINMENT
With the help of CO against PO mapping, the PO attainment is calculated. PO assessment is done by giving 80% weightage to direct assessment and 20% weightage to indirect assessment. Direct assessment is based on CO attainment, where 75% weightage is given to attainment through End Semester examination and 25% weightage is given to attainment through internal assessments. Indirect assessment is done through Graduate exit survey. PO Assessment Tools
Mode of Assessment Assessment Tool Description
Direct Attainment CO Assessment This is computed from the calculated CO (Weightage 80%) Attainment value for each Course Indirect Attainment Graduate Exit At the end of the programme, Graduate Exit (Weightage 20%) survey Survey is collected from the graduates and it gives the opinion of the graduates on attainment of Programme Outcomes Direct Attainment of POs for all Courses At the end of the each programme, the direct PO assessment is done from the CO attainment of all courses. The direct PO attainment for a particular course is determined from the attainment values obtained for each course outcome related to that PO and the CO-PO mapping values. For the evaluation and assessment of CO’s and PO’s, the same set of rubrics is used. Programme Articulation Matrix (PAM):
Course Course Title PO1 PO2 PO3 PO4 PO5 PO6 PO7 Code
Average Direct PO Attainment
Direct PO Attainment in %
Indirect Attainment of POs for all Courses
POs PO1 PO2 PO3 PO4 PO5 PO6 PO7
Graduate Exit survey Indirect PO Attainment
Indirect PO Attainment = 20% of PO Attainment from Graduate Exit survey
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Page 10 of 159 Attainments of POs for all Courses
POs PO1 PO2 PO3 PO4 PO5 PO6 PO7
Direct Attainment (Weightage 80%) Indirect Attainment (Weightage 20%)
Overall PO Attainment
Overall PO Attainment = 80% of Direct PO Attainment + 20% of Indirect PO Attainment III. ASSESSMENT PROCESS FOR PROGRAMME EDUCATIONAL OBJECTIVES The curriculum is designed so that all courses contribute to the achievement of PEOs. The marks secured by the students in these courses indicate the level of achievement of the PEOs. In addition, Alumnae survey, Placements of students and progression to higher studies also contribute to the attainment of PEOs.
Indicators Type of Data Assessment Assessment Responsible for Assessment collection Tool criteria entity Attainment frequency of PEO PO This is computed from the calculated PO PEO-1 Direct Assessment Attainment values for each Course PEO-2 Weightage PEO-3 70% PEO -4 Alumnae Once in a year, Alumnae Survey is PEO-1 Survey collected from the alumnae and it gives the PEO-2 Weightage opinion of the alumnae on attainment of PEO-3 10% Programme Outcomes and their PEO-4 achievements Placement Number of Once in a Placement PEO-1 Record students year cell PEO-2 Indirect Weightage Placed PEO-3 Weightage 10% PEO -4 30% Higher Number of Once in a Department PEO -1 Education students year PEO-2 Weightage opted for PEO-3 10% higher PEO-4 education
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Page 11 of 159 The attainment of the PEOs Direct Evaluation of Programme Outcomes (POs) of the concerned PEO
POs PEO1 PEO2 PEO3 PEO4 PO1 PO2 PO3 PO4 PO5 PO6 PO7 Average Direct PEO Attainment in %
Total Direct PEO Attainment Indirect Attainment of PEOs
PEOs PEO1 PEO2 PEO3 PEO4
Alumnae Survey
Average PEO Attainment from Alumnae Survey
Indirect Attainment from placement
Indirect Attainment from higher studies
Indirect PEO Attainment = 10% attainment of Alumnae survey + 10% Attainment from placement+ 10 % Attainment from higher studies Overall PEO Attainment = 70% of Direct PEO Attainment+ Indirect PEO Attainment Expected Level of Attainment for each of the Programme Educational Objectives
PEO Level of Attainment Value >=70% Excellent Value > = 60 and value < 70 Very good Value > = 50 and value < 60 Good Value > = 40 and value < 50 Satisfactory Value < 40 Not Satisfactory
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Page 12 of 159 Level of PEO attainment
Graduation Overall PEO Attainment Whether Expected level of Batch PEO is achieved?
Process of Redefining the PEOs: The college has always been involving the key stake holders in collecting information and suggestions with regard to curriculum development and curriculum revision. Based on the information collected the objectives of the programme are defined, refined and are inscribed in the form of PEO’s. The level of attainment of PEO’s defined earlier will be analyzed and will identify the need for redefining PEOs. Based on identified changes in terms of curriculum, regulations and PEOs, the administrative system like BOS, Academic Council and Governing Body involve appropriate actions.
IV. Eligibility Condition for Admission
1. For admission of Under Graduate Programmes, (U.G.) a candidate must have passed the Higher Secondary Examination of Tamil Nadu or an examination (like the CBSE) recognized by the universities as equivalent there to, who have opted Group I in Plus Two with the following subjects as part III Mathematics (200), Physics (200), Chemistry (200), Biology/Computer Science (200).
2. Based on the rank list prepared for a total of 800 marks in the above subjects and subject to the norms as specified by the Government, admission will be made.
3. The upper age limit for admission to U.G. Programmes will be 21 (twenty one) years as on 1 st July of the year of admission. For SC/ST/BC/MBC/DNC candidates and women candidates, the age limit is relaxed by 3 years. In the case of candidates selected under merit the upper age limit may be relaxed by the Principal. A relaxation of 5 years is permitted for physically handicapped as per G.O.Ms.No.239, dated 3-9-93.
V. Duration of the Programme:
The duration of the programme is three academic years. Each academic year consists of two semesters. The duration of a semester is 90 working days.
VI. Attendance:
The Rules regarding the attendance for regular classes for the candidates to appear for the End Semester Examinations are framed as given below:
a) Each student must put in a minimum attendance of 68 days (75% of 90 days per semester) so as to become eligible to appear for the End Semester examinations.
Shortage of Attendance:
b) Those students with an attendance of 67 days and less but 59 days (65%) and above can be permitted to appear for the End Semester Examinations provided, they get the condonation certificate from the Principal stating the proper reasons for the absence, within 5 days after the last working day of the concerned class. The certificate may be
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Page 13 of 159 obtained from the office on payment of penalty as per Madurai Kamaraj University norms.
c) In case of attendance with 58 days and less but 45 days (50%) and above, the students cannot appear for the End Semester Examinations of that semester but can appear for the next End Semester Examinations by obtaining special permission from the Principal providing necessary documents supporting the reasons for absence on payment of penalty as per Madurai Kamaraj University norms.
d) Students with an attendance of 44 days and less should repeat the whole semester.
Attendance for Part V
A student of the First or Second year undergraduate class should put in a minimum attendance of 75% for each semester (Total No. of hours as fixed by the concerned Officers / Staff in-charge) in anyone of the Co-Curricular activities namely NCC/ NSS/ Physical Education/ Extension Activities/Youth Red Cross/Social Service League/Red Ribbon Club/Citizen Consumer Club/Environmental Club to be eligible to get the degree.
In case of shortage of attendance the student has to complete the required attendance before the completion of the U.G Programme. If she fails to do so, the student can appear for the End Semester Examinations but she is ineligible to get the degree.
VII. Evaluation Procedure:
Evaluation of each theory course will be 25% for CIA and 75% for End Semester Examinations. Evaluation of each Practical Course will be 40% for CIA and 60% for End Semester Examinations. Project will be evaluated for 100% in the End Semester Examinations. A Mark Statement will be issued to every student at the end of every semester.
VIII. Passing Minimum:
For a pass in each course a student should secure a minimum of 35% marks in the End Semester Examinations and a minimum of 40% marks in aggregate (i.e. marks of CIA and End Semester Examinations put together).The same rule is applicable for Dissertation/Project Report and Viva – Voce.
Minimum credits to be earned for B.Sc. B.Sc Botany with Specialization In Plant Biotechnology Programme is 140 credits.
IX. Eligibility Condition for getting the Degree:
A Candidate undergoing the B.Sc. Degree Programme in B.Sc Botany with Specialization In Plant Biotechnology will be eligible for the award of degree in Botany with Specialization In Plant Biotechnology, if she completes the entire Programme and pass all the examinations prescribed for the Programme.
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Page 14 of 159 As per UGC guidelines, a student who is not able to complete the Programme within three years may be allowed for 2 years period beyond the three years duration to clear the backlog to be qualified for the degree.
X. Classification of Successful Candidates:
The successful candidates will be classified as per the details given in the table below:
CGPA GRADE CLASSIFICATION OF FINAL RESULT 9.50000 -10.00000 O+ 9.00000 -9.499999 O 8.50000-8.99999 D++ 8.00000 -8.49999 D+ 7.50000 -7.99999 D 7.00000 -7.49999 A++ 6.50000 -6.99999 A+ First Class 6.00000 -6.49999 A 5.50000 -5.99999 B+ Second Class 5.00000 -5.49999 B 4.50000 -4.99999 C+ Third Class 4.00000 -4.49999 C 0.00000 -3.99999 U Re -appear
XI. Award of Ranks:
Candidates who qualify themselves for the respective Degree Programme, passing all the examinations in the first attempt are eligible for ranking based on the CGPA gained in the Major & Allied courses.
∑ Ci Gi CUMULATIVE GRADE POINT AVERAGE [CGPA] = i ∑ Ci i
Sum of the multiplica tion of grade points by the respective credits of the course cleared in the entire programme
CGPA = Sum of the credits of all the courses cleared in the programme
Ci = Credits earned for course i in any semester Gi = Grade point obtained for course i in any semester ∑ = Summation of all courses cleared in a semester in the case of GPA i and all courses cleared in all semesters in the case of CGPA.
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Page 15 of 159 XII. Other Provisions :
1. In the Mark Statement, ‘AA’ will be marked against the courses for which the candidate was absent for the examination.
2. If a candidate is found indulging in malpractice, she will be expelled from the examination hall right away and debarred from appearing in all examinations of that particular semester. She can be allowed to take up examination only in the consecutive semester.
3. The courses she has already appeared during that semester will not be considered.
4. A student can appear for any number of arrear courses.
5. Repeat Examinations will be conducted for the final semester papers within a month after the publication of final semester results.
6. Revaluation is permitted.
XIII. Transitory Provisions:
Students from other institutions will be granted year exemption alone and she has to appear and pass all the courses of all semesters under CBCS pattern in order to get the consolidated Statement of Marks/Grade.
Those students who have discontinued in the middle of the Programme may be admitted in the respective semester if they want to rejoin and complete the Programme, provided they had not got their transfer certificate.
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Page 16 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY (UG PROGRAMME) Allotment of Hours and Credits (For those who have joined in June 2017 and later )
Semester Subject Total I II III IV V VI No.of Credits Part I - Tamil language course 6(3) 6(3) 6(3) 6(3) - - 12 Part II – English Language Course 6(3) 6(3) 6(3) 6(3) - - 12 Part III – Major and Allied Courses: Course I 4(4) 5(5) 4(4) 5(5) 6(5) 6(5) Theory Course II 3(3) - 3(3) - 5(5) 5(5) a) Major Course III - - - - 5(5) 5(5) Lab I 3(*) 3(4) 3(*) 3(4) 6(5) 6(5) Practical Lab II - - - - 3(*) 3(3) b)Allied I & II Theory 4(4) 4(4) 4(4) 4(4) - - Lab 2(*) 2(2) 2(*) 2(2) - - Total No.of Credits 11 15 11 15 20 23 95 Part IV – Non Major/ Value Added Courses Peace Education 2(2) - - - - - Environmental Studies - 2(2) - - - - i) NME I - - 2(2) - - - ii) NME II - - - 2(2) - - i) Computer Literacy - 2(2) - - - - ii) Discipline Specific Course - - - 2(2) - - iii) Career Guidance and Subject Viva - - - 2(2) - iv) Women Studies - - - - 2(2) - v) Skill Based Courses :Self Employment/ - - - - - 2(2) Job Oriented courses – Theory vi) Skill Based Courses :Self Employment/ Job Oriented courses – Practical/Field - - - - - 2(2) work/ Project f) Library and Information Science - - - - 1(*) 1(*) Total No.of Credits 2 4 2 4 4 4 20 Part V - Extension Activities - Physical Education & Social Awareness Programme NSS/NCC/Physical Education/Extension Activities/Youth Red Cross/Social Service (*) (1) - - - - 1 League/Red Ribbon Club/Envirronmental club/Citizen Consumer club Total 140
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Page 17 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN (AUTONOMOUS), SIVAKASI. DEPARTMENT OF BOTANY (UG PROGRAMME) (For those who have joined in June 2017 and later)
Marks Allotted
Course Sem. Course Title Code Per Week Per Internal External Teaching Teaching Hours Credits ofDuration (Hrs.)Exam. Total
PART III – MAJOR AND ALLIED COURSES I GLBP11 Plant Diversity-I 4 4 3 25 75 100 GLBP12 Cell Biology and Plant Anatomy 3 3 3 25 75 100 GLBP1A Animal Biotechnology I 4 4 3 25 75 100 II GLBP21 Plant Diversity-II 5 5 3 25 75 100 I & II GLBP2L Practical I 3+ 3 4 3 40 60 100 II GLBP2A Animal Biotechnology II 4 4 3 25 75 100 I & II GLBP2AL Allied Practical 2 + 2 2 3 40 60 100 GLBP31 Embryology and Plant tissue culture 4 4 3 25 75 100 III GLBP32 Genetics 3 3 3 25 75 100 IV GLBP41 Biochemistry 5 5 3 25 75 100 III & IV GLBP4L Practical II 3+3 4 3 40 60 100 GLBP51 Taxonomy of Angiosperms 6 5 3 25 75 100 V GLBP5EA Elective I 5 5 3 25 75 100 GLBP5EB Elective II 5 5 3 25 75 100 GLBP5L Practical III 3 + 3 3 3 40 60 100 GLBP61 Plant Physiology 6 5 3 25 75 100 GLBP62 Biotechnology 5 5 3 25 75 100 VI GLBP6EC Elective III 5 5 3 25 75 100 GLBP6L1 Practical IV 3 + 3 5 3 40 60 100 GLBP6L2 Practical V 3 + 3 3 3 40 60 100 ELECTIVE COURSES GLBP5E1 Microbiology and Plant Pathology 5 5 3 25 75 100 V GLBP5E2 Horticulture 5 5 3 25 75 100 GLBP5E3 Natural Resources 5 5 3 25 75 100 GLBP5E4 Herbal Medicine 5 5 3 25 75 100 VI GLBP6E1 Ecology and Biodiversity 5 5 3 25 75 100 GLBP6E2 Economic Botany 5 5 3 25 75 100 ALLIED COURSE II ( for chemistry major students ) III GLBP3A Fundamentals of Biochemistry 4 4 3 25 75 100 IV GLBP4A Microbiology and Biotechnology 4 4 3 25 75 100 III & IV GLBP4AL Allied Practical 2 + 2 2 3 40 60 100 Part IV – NON MAJOR ELECTIVE I & II (open option) III GLBP3N Freshwater Aquaculture 2 2 2 25 75 100 IV GLBP4N Sericulture 2 2 2 25 75 100 DISCIPLINE SPECIFIC COURSE IV GLBP4DS Food processing technology 2 2 2 25 75 100 SELF EMPLOYMENT/ JOB ORIENTED COURSE VI GLSE68 Herbal Cosmetics 2 2 2 25 75 100 GLSE68L Herbal Cosmetics Lab 2 2 3 40 60 100
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Page 18 of 159 B.Sc. Botany with specialization in Plant Biotechnology Programme Articulation Matrix (PAM) - Weights
Sem Course Course title PO1 PO2 PO3 PO4 PO5 PO6 PO7 code GLGT11/ rq;f ,yf;fpaKk; 15 27 3 5 3 1 1 GLGH11/ ciueilAk ;/ Hindi GLGF11 Language Course - I/ French Language Course - I I GLGE11 Communicative English -I 11 12 3 27 - - 9 GLBP11 Plant Diversity-I 33 12 3 3 - - - GLBP12 Cell Biology and Plant Anatomy 33 15 3 2 4 - - GLBP1A Animal Biotechnology I 45 8 15 4 - 1 - GLPE11 Peace education 19 13 - 16 24 21 9 GLGT21/ fhg;gpa ,yf;fpaKk; 15 7 11 5 3 3 2 GLGH21/ GjpdKk ;/ GLGF21 Hindi Language Course -II / French Language Course - II GLGE21 Communicative English-II 15 15 3 15 - - 6 GLBP21 Plant Diversity-II 27 11 4 4 1 - - II GLBP2L Practical I 33 37 11 7 - - - GLBP2A Animal Biotechnology II 45 9 15 7 - - - GLBP2AL Allied Practical 13 8 45 10 15 - - GLES21 Environmental studies 39 12 6 3 3 15 2 GLCL23 Introduction to Computer & MS 33 10 - 12 - - 9 Office GLGT31/ rka ,yf;fpaKk; ehlfKk ;/ 21 9 18 5 3 3 4 GLGH31/ Hindi GLGF31 Language Course –III / French Language Course - III III GLGE31 Communicative English-III 15 15 - 15 - - 9 Embryology and Plant tissue 31 10 9 3 - - - GLBP31 culture GLBP32 Genetics 33 19 3 6 - - 1 GLCH3A Fundamentals of chemistry 19 15 19 11 3 - - GLNM Non Major Elective I 19 14 - 9 - - - GLGT41/ ftpij ,yf;fpaKk; 15 39 12 5 3 6 4 GLGH41/ rpWfijAk ;/ Hindi Language GLGF41 Course -IV/ French Language Course - IV GLGE41 Communicative English -IV 15 15 3 12 - - 9 IV GLBP41 Biochemistry 25 10 9 6 2 - - GLBP4L Practical II 21 25 18 13 6 - - GLNM Non Major Elective II 19 14 - 9 - - - GLBP4DS Food processing technology 19 3 18 4 7 - - GLCH4A Physical and Industrial 10 24 15 11 - - 3 Chemistry GLCH4AL Volumetric Estimation 16 18 3 11 - - - GLBP51 Taxonomy of Angiosperms 33 24 4 6 - - 1 GLBP5EA Elective I 27 15 5 9 - - - GLBP5EB Elective II 31 19 18 7 4 - - V GLBP5L Practical III 31 39 25 21 12 - -
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Page 19 of 159 GLGV51 Career Guidance and Subject Viva 22 24 3 1 - - 2 GLWS51 Women Studies 45 18 - 9 13 3 30 VI GLBP61 Plant Physiology 33 27 5 5 6 - - GLBP62 Biotechnology 33 27 7 2 2 - - GLBP6EC Elective III 33 15 13 6 7 - - GLBP6L1 Practical IV 33 39 33 13 13 - - GLBP6L2 Practical V 21 33 19 9 6 - - Self Employment/ Job Oriented 19 14 - 9 - - 6 GLSE/GLJO courses – Theory Self Employment/ Job Oriented 19 14 - 9 - - 6 GLSE/GLJO courses – Practical/Field work/Project Total Weightage of all courses contributing to POs 1034 734 381 346 140 53 113
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Page 20 of 159 B.Sc. Botany with specialization in Plant Biotechnology Programme Articulation Matrix - Weighted Percentage
Sem Course Course title PO1 PO2 PO3 PO4 PO5 PO6 PO7 code GLGT11/ rq;f ,yf;fpaKk; GLGH11/ ciueilAk;/ Hindi GLGF11 Language Course- I/ French Language Course - I 1.45 3.68 0.79 1.45 2.14 1.89 0.88 GLGE11 Communicative I English-I 1.06 1.63 0.79 7.8 0 0 7.96 GLBP11 Plant Diversity -I 3.19 1.63 0.79 0.87 0 0 0 Cell Biology and Plant GLBP12 Anatomy 3.19 2.04 0.79 0.58 2.86 0 0 GLBP1A Animal Biotechnology I 4.35 1.09 3.94 1.16 0 1.89 0 GLPE11 Peace education 1.84 1.77 0 4.62 17.14 39.62 7.96 GLGT21/ fhg;gpa GLGH21/ ,yf;fpaKk; GLGF21 GjpdKk;/ Hindi Language Course -II / French Language 1.45 0.95 2.89 1.45 2.14 5.66 1.77 Course - II GLGE21 Communicative II English-II 1.45 2.04 0.79 4.34 0 0 5.31 GLBP21 Plant Diversity -II 2.61 1.5 1.05 1.16 0.71 0 0 GLBP2L Practical I 3.19 5.04 2.89 2.02 0 0 0 Animal GLBP2A Biotechnology II 4.35 1.23 3.94 2.02 0 0 0 GLBP2AL Allied Practical 1.26 1.09 11.81 2.89 10.71 0 0 GLES21 Environmental studies 3.77 1.63 1.57 0.87 2.14 28.3 1.77 GLCL23 Introduction to computer & MS Office 3.19 1.36 0 3.47 0 0 7.96 GLGT31/ rka ,yf;fpaKk; GLGH31/ ehlfKk;/ Hindi GLGF31 Language Course –III / French Language 2.03 1.23 4.72 1.45 2.14 5.66 3.54 Course - III III GLGE31 Communicative English-III 1.45 2.04 0 4.34 0 0 7.96 Embryology and Plant GLBP31 tissue culture 3 1.36 2.36 0.87 0 0 0 GLBP32 Genetics 3.19 2.59 0.79 1.73 0 0 0.88 GLCH3A Fundamentals of chemistry 1.84 2.04 4.99 3.18 2.14 0 0 GLNM Non Major Elective I 1.84 1.91 0 2.6 0 0 0 GLGT41/ ftpij ,yf;fpaKk; GLGH41/ rpWfijAk;/ Hindi GLGF41 Language Course -IV/ IV 1.45 5.31 3.15 1.45 2.14 11.32 3.54 French Language Course – IV
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Page 21 of 159 GLGE41 Communicative English-IV 1.45 2.04 0.79 3.47 0 0 7.96 GLBP41 Biochemistry 2.42 1.36 2.36 1.73 1.43 0 0 GLBP4L Practical II 2.03 3.41 4.72 3.76 4.29 0 0 GLNM Non Major Elective II 1.84 1.91 0 2.6 0 0 0 Food processing GLBP4DS technology 1.84 0.41 4.72 1.16 5 0 0 GLCH4A Physical and Industrial Chemistry 0.97 3.27 3.94 3.18 0 0 2.65 GLCH4AL Volumetric Estimation 1.55 2.45 0.79 3.18 0 0 0 Taxonomy of GLBP51 Angiosperms 3.19 3.27 1.05 1.73 0 0 0.88 GLBP5EA Elective I 2.61 2.04 1.31 2.6 0 0 0 GLBP5EB Elective II 3 2.59 4.72 2.02 2.86 0 0 V GLBP5L Practical III 3 5.31 6.56 6.07 8.57 0 0 Career Guidance and GLGV51 Subject Viva 2.13 3.27 0.79 0.29 0 0 1.77 GLWS51 Women Studies 4.35 2.45 0 2.6 9.29 5.66 26.55 GLBP61 Plant Physiology 3.19 3.68 1.31 1.45 4.29 0 0 GLBP62 Biotechnology 3.19 3.68 1.84 0.58 1.43 0 0 GLBP6EC Elective III 3.19 2.04 3.41 1.73 5 0 0 GLBP6L1 Practical IV 3.19 5.31 8.66 3.76 9.29 0 0 GLBP6L2 Practical V 2.03 4.5 4.99 2.6 4.29 0 0
Self Employment/ Job VI GLSE/ Oriented courses – GLJO Theory 1.84 1.91 0 2.6 0 0 5.31 Self Employment/ Job GLSE/ Oriented courses – GLJO Practical/Field work/Project 1.84 1.91 0 2.6 0 0 5.31 Total Weighted percentage of all courses contributing to POs 100 99.97 100.01 100.03 100 100 99.96
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Page 22 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY MAJOR COURSE SEMESTER I GLBP11 - PLANT DIVERSITY- I (For those admitted in June 2017 and later)
Contact hours per week 04 Total number of hours per semester 60 Number of credits 04
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: summarize the characters, classification and economic importance of lower plants. CO2: illustrate the thallus structure and reproduction of algae. CO3: analyze the structure and reproduction of fungi. CO4: examine the thallus structure and reproduction of lichens. CO5: explain the structure and reproduction of bryophytes.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 ------CO2 9 3 - - - - - CO3 9 3 - - - - - CO4 9 3 3 - - - - CO5 3 3 - 3 - - - Weightage of 33 12 3 3 - - - the course Weighted 3.19 1.63 0.79 0.87 - - - percentage of Course contribution to POs
Unit – I (12 hrs) Classification of Algae based on Fritsch (1935). Occurrence, structure, reproduction and life cycle of the following types (Development of sex organs are excluded). • Cyanophyceae – Nostoc • Chlorophyceae – Volvox
Unit – II (12 hrs) Occurrence, structure, reproduction and life cycle of the following types (Develpoment of sex organs are excluded). • Phaeophyceae – Sargassum
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Page 23 of 159 • Rhodophyceae – Polysiphonia Economic importance of Algae – beneficial - Algae as food, biofertilizer, source of agar, diatomite and role of Algae in industries and harmful effects.
Unit – III (12 hrs) Classification of Fungi based on Alexopoulos (1962). Occurrence, structure, reproduction and life cycle of the following types ((Develpoment of sex organs are excluded) • Ascomycetes – Saccharomyces • Basidiomycetes – Puccinia
Unit – IV (12 hrs) Economic importance of fungi- Beneficial effects - Fungi as food, industry, pharmaceuticals and agriculture and harmful effects. Lichens – Classification - ascolichens, basidiolichens. structure - External and internal structure - homomerous and heteromerous thalli. Structures associated with lichen thallus. Economic importance of lichens. Usnea – Structure, reproduction - asexual and sexual reproduction.
Unit – V (12 hrs) Classification of Bryophytes based on Rothmaler (1951). Structure, reproduction and life cycle of the following types (need not study the development of sex organs, gametophyte and sporophyte) • Hepaticopsida – Marchantia • Bryopsida – Funaria
Text Book: 1. Singh,V., Pandey P.C., and Jain D.K. (1998) A Text Book of Botany, Rastogi Puplications , II Revised Edition. Unit I - 19-21, 42-44 and 60-66. Unit II - 152-158 and 178-183. Unit III- 10, 59-66 and 109-116. Unit IV- 153-168. Unit V – 21-31 and 86-97.
Reference Books: 1. Chopra, G.D., (1980), A Text Book of Algae , S. Nagin & Co., New Delhi, XVII Edition. 2. Vashista, B.R., (1994), Bryophyta , S. Chand and Co., New Delhi, VIII Edition. 3. Vashista, B.R., (1990), Fungi , S. Chand and Co., New Delhi, IX Edition.
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Page 24 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY MAJOR COURSE SEMESTER I GLBP12 – CELL BIOLOGY AND PLANT ANATOMY (For those admitted in June 2017 and later)
Contact hours per week : 03 Total number of hours per semester : 45 Number of credits : 03
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: summarize the structure and functions of different components of cell. CO2: analyze the process of cell divisions and its significances. CO3: identify the types of tissue and its functions. CO4: compare the tissue composition of different parts of a plant. CO5: explain the normal and anomalous secondary growth in plants.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 - - 1 3 - - CO2 9 3 1 1 - - - CO3 3 ------CO4 9 3 1 - 1 - - CO5 9 9 1 - - - - Weightage of 33 15 3 2 4 - - the course Weighted percentage of Course 3.19 2.04 0.79 0.58 2.86 - - contribution to Pos
Unit – I (9 hrs) Ultrastructure of a plant cell. Structure of cell wall – Primary and secondary. Plasma membrane - structure (fluid mosaic model alone) and functions. Structure and functions of the following cell organelles – mitochondria and chloroplast.
Unit – II (9 hrs) Structure and functions of endoplasmic reticulum, ribosomes, dictyosomes and nucleus. Cell divisions – mitotic and meiotic cell divisions.
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Page 25 of 159 Unit – III (9 hrs) Meristem – definition, classification. Theories regarding root and shoot apices – apical cell theory, histogen theory, tunica - corpus theory, quiescent centre concept and korper - kappe theory. Structure and functions of simple permanent tissues - parenchyma, collenchyma and sclerenchyma.
Unit – IV (9 hrs) Structure and functions of complex permanent tissues - xylem and phloem. Primary structures of dicot root and stem – Helianthus, monocot root and stem - Zea mays .
Unit – V (9 hrs) Normal secondary growth in dicot root and stem. Anomalous secondary growth in dicot stem - Boerhaavia and monocot stem - Dracaena . Anatomy of dicot leaf - Mangifera and monocot leaf - Zea mays .
Text Books: 1. Veer Bala Rastogi, (1979-1980), Introductory Cytology , Kedar Nath Ram Nath, Meerut, V Edition. Unit I – Page No: 58-64, 133-137, 108, 117-118, 178-183, 193-195 and 218-229. Unit II – Page No: 142-146, 169-177, 151-160, 236-246, 337-340 and 346- 358. 2. Pandey, B.P., (Reprint 2013), Plant Anatomy , S. Chand and Co., New Delhi. Unit III – Page No: 89-100 and 122-129. Unit IV – Page No: 101-112, 181-191, 213-218 and 229-230. Unit V – Page No: 236 – 252, 204-209, 268-273, 291-293 and 304-305.
Reference Books: 1. Katherine Esau. (1972), Plant anatomy , Wiley Eastern Pvt. Ltd., New Delhi, II Edition. 2. Verma, P.S. and V.K. Agarwal, (1985), Cytology , S. Chand and Co., New Delhi, V Edition.
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Page 26 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY ALLIED COURSE SEMESTER I GLBP1A - ANIMAL BIOTECHNOLOGY I (For those admitted in June 2017 and later)
Contact hours per week 04 Total number of hours per semester 60 Number of Credits 04
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: demonstrate the techniques pertaining to animal cell culture. CO2: design and evaluate the techniques for development and maintenance of cell lines. CO3: develop animal cell culture products. CO4: examine the methods of transgenesis. CO5: unravel the information pertaining to transgenesis and to relate transgenic animals with ethical issues.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 - 3 - - - - CO2 9 3 3 1 - - - CO3 9 1 3 - - - - CO4 9 3 3 3 - - - CO5 9 1 3 - - 1 - Weightage of 45 8 15 4 - 1 - the course Weighted percentage of Course 4.35 1.09 3.94 1.16 - 1.89 - contribution to POs
Unit - I (12 hrs) Animal cell culture: Substrate, Culture media - natural media, artificial media. Cell culture practices: Preparation and sterilization of substrate and medium, Isolation, disaggregation of explants, subculture.
Unit - II (12 hrs) Cell lines: Development and maintenance of cell lines. Large scale culture of cell lines - Monolayer cultures, suspension cultures, immobilized cultures.
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Page 27 of 159 Unit - III (12 hrs) Animal cell culture products: Preparation of two major categories of viral vaccines, The mechanism of protection by Interferons, Use of cell lines in the production of recombinant protein – insulin. Construction of hybrid antibodies (antibody engineering ).
Unit - IV (12 hrs) Transgenesis: Methods of transferring genes - physical, chemical and biological methods (Calcium phosphate precipitation, direct microinjection, retro virus infection, lipofection, particle gun delivery, electroporation and embryonic stem cell transfer).
Unit - V (12 hrs) Transgenic animals: Introduction, Gene Targeting in Mice, Knock out and knock in technology, Transgenic cow, Transgenic pig, Transgenic goat, Transgenic bird, Transgenic fish and Transgenic insects. Ethical issues pertaining to transgenic animals.
Text Book: 1. Singh , B.D ( 2008 ), Biotechnology , Kalyani Publishers , New Delhi Unit I: Page No: 115 – 127. Unit II: Page No: 128 – 138. Unit III: Page No: 169 – 179. Unit V: Page No: 228 – 232. Reference Books: 1. Sasidhara, R (2006), Animal Biotechnology , MJP Publisher, Chennai. Unit - V: Page Number: 133 – 163. 2. Spier, R.E. and Griffiths, J.B (1988), Animal Cell Biotechnology , Academic Press, New York. 3. Butler, M (1987), Animal Cell Technology , Principles and Products , Open University Press, New York 4. Brown , T.A (2001), Gene Cloning and Analysis , Blackwell science Ltd.,
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Page 28 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY MAJOR COURSE SEMESTER II GLBP21 - PLANT DIVERSITY- II (For those admitted in June 2017 and later)
Contact hours per week 05 Total number of hours per semester 75 Number of credits 05 Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: recall the general characters and classification of Pteridophytes and Gymnosperms. CO2: discuss the structure and reproduction of Pteridophytes. CO3: analyse the morphology, anatomy, reproduction and economic importance of Gymnosperms. CO4: explain the formation and types of fossil and their existence in various eras. CO5: examine the structure and reproduction of fossil.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 1 - - - - - CO2 9 3 - - 1 - - CO3 9 3 - 3 - - - CO4 3 1 1 1 - - - CO5 3 3 3 - - - - Weight age of 27 11 4 4 1 - - the course Weighted 2.61 1.5 1.05 1.16 0.71 - - percentage of Course contribution to POs
Unit – I (15 hrs) General characters of Pteridophytes – classification of Pteridophytes by G.M.Smith, 1933. Structure and reproduction of the following types. (development of sex organs, gametophyte and sporophyte are not included) (a) Psilotum (b) Lycopodium (c) Equisetum
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Page 29 of 159 Unit – II (15 hrs) Structure and reproduction of the following types. (development of sex organs, gametophyte and sporophyte are not included) (a) Pteridium (b) Marsilea (c) Azolla
Unit – III (15 hrs) General characters of Gymnosperms – classification of Gymnosperms by C.J.Chamberlain, 1910. Economic importance of Gymnosperms. Pinus – Habit – internal structure - root, stem and needle. Reproduction – asexual and sexual reproduction, economic importance (developmental aspects need not be discussed).
Unit – IV (15 hrs) Gnetum – Habit - internal structure - root, stem and leaf. Secondary growth in root and stem. Reproduction – structure of male strobilus, female strobilus, ovule, seed, and germination of seed. Angiospermic characters of Gnetum (developmental aspects need not be discussed).
Unit – V (15 hrs) Formation of fossils, fossil types - compressions, impressions, incrustations, petrifactions, compactions. Geological time scale. Structure and reproduction of the following fossil types. (a) Rhynia (b) Lepidodendron (c) Williamsonia Text Book: 1. Singh,V., Pandey P.C., and Jain D.K. (1998) A Text Book of Botany, Rastogi Puplications , II Revised Edition. Unit I - 1-13, 29-51 and 88-100. Unit II - 125-134 and 146-173. Unit III- 1 -10 and 37 -58. Unit IV- 84 -99. Unit V – 14-28, 14-17 and 52-55. Reference Books: 1. Parihar N.S., The Biology and Morphology of Pteridophytes , II Edition, 1992. 2. Vashista, P.C., Pteridophyta , S. Chand & Co, New Delhi, IV Edition, 1990. 3. Chamberlain, C.J., Gymnosperms, CBS Publishers & Distributors, Delhi, I Edition, 1986. 4. Vashista, P.C., Gymnosperms Volume V , S. Chand & Co, New Delhi, XV Edition, 1996. 5. Pandey, B.P., College Botany Vol. II , S. Chand and Co., New Delhi, VII Edition, 1993.
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Page 30 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY CORE COURSES (MAJOR) SEMESTER I & II GLBP2L - PRACTICAL I (Plant Diversity - I, Cell Biology and Plant Anatomy and Plant Diversity - II) (For those admitted in June 2017 and later)
Contact hours per week : 03 +03 Total number of hours per semester : 45 + 45 Number of credits 04
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: identify the specimens and slides. CO2: illustrate the preparation of whole mount. CO3: analyze the structure and functions of cell organelles. CO4: examine the process of cell division in plants. CO5: formulate suitable technique for the study of internal structure of a plant part.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 9 1 1 - - - CO2 9 9 3 1 - - - CO3 3 1 1 1 - - - CO4 9 9 3 3 - - - CO5 9 9 3 1 - - - Weight age of 33 37 11 7 - - - the course Weighted 3.19 5.04 2.89 2.02 - - - percentage of Course contribution to POs
Plant Diversity - I 1. Make suitable whole mount preparation of the following types Nostoc Volvox 2. Make suitable slide preparation of the following Sargassum – axis Puccinia - Uredial and Telial stage 3. Spot at sight Saccharomyces Lichen - habit - crustose, foliose and fruticose and V.S. of apothecium.
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Page 31 of 159 Marchantia - gametophyte Funaria - sporophyte Polysiphonia - Cystocorp Cell Biology and Plant Anatomy 1. Smear the onion root tip and identify different stages of mitosis. 2. Making suitable slide preparation of the following Tridax - T.S. of old root and stem Boerhaavia - T.S. of old stem Dracaena – T.S. of old stem Mangifera – T.S. of leaf Zea mays – T.S. of leaf Spot at sight Ultrastructure of a plant cell, mitochondria, chloroplast, endoplasmic reticulum and nucleus. Submission of observation note book. Plant Diversity - II 1. Make suitable slide preparation of the following Lycopodium – T.S. of stem. Equisetum - T.S. of stem. Pteridium - T.S. of stem. Marsilea - T.S. of petiole Pinus - T.S.of stem, T.L.S and R.L.S, T.S. of needle. Gnetum - T.S. of stem. 3. Spot at sight Psilotum – synangium. Lycopodium – L.S of cone. Equisetum - habit. Pteridium – habit. Marsilea – habit and T.S of petiole and sporocarp. Pinus - L.S. of male cone, L.S. of female cone and L.S. of ovule. Gnetum - L.S. of male cone, L.S. of female cone and L.S. of ovule. Rhynia – T.S of rhizome, T.S. of stem and L.S of Sporangium. Lepidodendron - T.S. of root and T.S. of young axis. Williamsonia – habit.
Submission of observation note book.
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Page 32 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY ALLIED COURSE SEMESTER II GLBP2A - ANIMAL BIOTECHNOLOGY II (For those admitted in June 2017 and later)
Contact hours per week 04 Total number of hours per semester 60 Number of Credits 04
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: analyze the concepts of Human Genome Project. CO2: classify and characterize the methods of gene therapy. CO3: evaluate the basics of IVF Technology in man and cattle. CO4: demonstrate hybridoma technology along with its therapeutic and diagnostic applications. CO5: summarize stem cell biology and to evaluate its biomedical applications.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 1 3 1 - - - CO2 9 3 3 1 - - - CO3 9 3 3 3 - - - CO4 9 1 3 1 - - - CO5 9 1 3 1 - - - Weightage of 45 9 15 7 - - - the course Weighted percentage of Course 4.35 1.23 3.94 2.02 - - - contribution to POs
Unit - I (12 hrs) Human Genome Project (HGP): Milestones of HGP, Types and sequences in the Human Genome, Impact of HGP. Potential Benefits of HGP. Ethical, Legal and Social issues of HGP.
Unit - II (12 hrs) Gene therapy: Introduction, Germ line Gene therapy, Somatic Cell gene therapy, Augmentation Therapy, Targeted Gene Transfer, Target cells, Gene Transfer Techniques, Gene therapy for Human Diseases - Cancer.
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Page 33 of 159 Unit - III (12 hrs) In vitro Fertilization (IVF) in Human ; Embryo Transfer (ET) in Human, Super ovulation, IVF and ET in Farm animals e.g., Cow. Embryo Transfer in Cattle. Applications of Embryo Transfer Technology, Limitations.
Unit - IV (12 hrs) Hybridoma Technology: Methodology for Production of Monoclonal antibodies, Techniques of cell fusion, Selection and Screening of Positive Hybrids, Purification. Therapeutic and Diagnostic Applications of Monoclonal antibodies. Abzymes (Monoclonal Antibodies as Enzymes).
Unit - V (12 hrs) Stem cell Biology: Embryonic stem cells, Adult stem cells, Human embryonic stem cell culture, Procedure for therapeutic cloning, Biomedical Applications of stem cells.
Reference Books: 1. Prakash S.Lohar (2009), Bioinformatics, MJP Publisher, Chennai. Unit - I: Page No: 499 – 508 2. Dubey R.C (2009), A Text Book of Biotechnology , S. Chand & Company Ltd., New Delhi. Unit - II: Page No: 163 – 171. 3. Sasidhara, R (2006), Animal Biotechnology , MJP Publisher, Chennai. Unit - II: 239 – 248. Unit - V: Page No: 431 - 436. 4. Singh, B.D (2008 ), Biotechnology , Kalyani Publishers, New Delhi Unit - III: Page No: 413 – 414. 5. Gupta, P.K (2007), Elements of Biotechnology , Rastogi Publications, Meerut. Unit - III: Page No: 158 – 168. 6. Rema, L.P (2006), Applied Biotechnology , MJP Publisher, Chennai. Unit - IV: Page No: 155 -169. 7. Sudha Gangal, (2007), Principles and Practice of Animal Tissue Culture , University Press, Hyderabad. Unit - V: Page No: 128 - 135.
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Page 34 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY ALLIED COURSE SEMESTER I & II GLBP2AL – ALLIED PRACTICAL (for those admitted in June 2017 and later)
Contact hours per week : 02+02 Total number of hours per semester : 60 Number of Credits : 02
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: design the animal cell culture laboratory and adopt biosafety practices. CO2: demonstrate animal cell culture trials. CO3: prepare animal cell culture products. CO4: estimate the microbial load and their sensitivity to antibiotics. CO5: handle equipments.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 1 9 1 3 - - CO2 3 3 9 3 3 - - CO3 1 1 9 3 3 - - CO4 3 3 9 3 3 - - CO5 3 - 9 - 3 - - Weightage of 13 8 45 10 15 - - the course Weighted percentage of Course 1.26 1.09 11.81 2.89 10.71 - - contribution to POs
• Animal Cell Culture Laboratory - Design • Biosafety Practices • Preparation of Animal Cell Culture Media • Methods of Sterilization - Dry Heat Sterilization • Moist Heat Sterilization - Membrane Filtration - UV radiation • Serial Dilution and Plating • Animal Cell Culture - Preparation of Explants - Monolayering of Liver Cells
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Page 35 of 159 • Preparation of Vaccines - Heat Killed Vaccine - Formalin Killed Vaccine - Soluble Antigen - Cellular Antigen - Adjuvants • Immobilization of Cells • Methods of preservation and maintenance of Cell Lines • Antimicrobial Sensitivity Test - Kirby Bauer Method • Spotters References: 1. Sudha Gangal (2007) , Principles and Practice of Animal Tissue Culture , University Press, Hyderabad.
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Page 36 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY MAJOR COURSE SEMESTER III GLBP31 – EMBRYOLOGY AND PLANT TISSUE CULTURE (For those admitted in June 2017 and later)
Contact hours per week 04 Total number of hours per semester 60 Number of credits 04
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: explain the stages of microsporogenesis and megasporogenesis. CO2: analyze the process of fertilization and formation of endosperm. CO3: discuss about the embryo, polyembryony and apomixis in angiosperms. CO4: summarize the concepts and fundamental requirements for plant tissue culture. CO5: examine the regeneration of plantlets various through tissue culture techniques.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 1 1 - - - - - CO2 3 ------CO3 9 3 3 - - - - CO4 9 3 3 - - - - CO5 9 3 3 3 - - - Weightage of 31 10 9 3 - - - the course Weighted 3.0 1.36 2.36 0.87 - - - percentage of Course contribution to POs
Unit – I (12 hrs) Microsporogenesis - structure and development of microsporangium - anther wall - tapetum types and its functions. Sporogenous tissue. Structure and development of male gametophyte. Formation of vegetative and generative cell and its functions. Megasporogenesis - structure - development and types of megasporangium- crassinucellate, tenuinucellae type. Different types of ovules based on position of micropyle and funiculus. Structure, development and types of female gametophyte - Polygonum, Allium and Peperomia types.
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Page 37 of 159 Unit – II (12 hrs) Fertilization - germination of pollen grain - pollen tube growth - path of pollen tube - entry of pollen tube into the ovule - discharge of gametes - double fertilization and its significance. Endosperm - free nuclear, cellular, helobial and ruminate endosperm and its functions.
Unit – III (12 hrs) Development of dicot embryo - Capsella type. Development of monocot embryo - Luzula type. Polyembryony - classification - causes - types - cleavage polyembryony and embryos from cells of embryo sac other than egg - more than one embryo sac in the same ovule - activation of some sporophytic cells of the ovule. Apomixis - causes - vegetative reproduction - agamospermy - adventive embryony - diplospory - apospory. Significance of apomixis.
Unit – IV (12 hrs) Basic requirements for Tissue culture laboratory. Maintenance of aseptic condition in laboratory. Totipotency of cells. Nutrient Media - composition and preparation of MS medium. Collection of explant materials - Surface sterilization of explant materials-inoculation of explants-incubation of culture flasks-sub culture of callus.
Unit – V (12 hrs) Regeneration of plants from callus-Organogenesis – embryogenesis – direct and indirect method. Somatic embryogenesis. Anther culture and its application. Micropropagation. Somoclonal variation – role in crop improvement. Artificial seeds- types-production-uses and advantages.
Text Books: 1. Bhojwani S.S. and S.P. Bhatnagar, (2011). The embryology of Angiosperms , Vikas Publishing House Pvt. Ltd., New Delhi, V Edition. Unit - I - Page No: 13 - 38, 62 - 76 and 82 - 87. Unit - II - Page No: 124 - 147 and 177 - 198. Unit - III - Page No: 204 - 214; 235 - 244, 249 - 250 and 253 - 261. 2. Kumaresan V. (2009). Biotechnology, Saras publication Nagercoil . Reprint Unit IV - Page No: 435 – 445. Unit V - Page No: 446 - 448; 451 - 452,456 – 462.
Reference Books: 1. Maheswari P. (1990). An introduction to Embryology of Angiosperms , Tata Mc Graw Hill, New Delhi, XI Edition. 2. Ramawat K.G. (2008). Plant Biotechnolgy , S.Chand & Co Ltd., New Delhi. 3. Razdan M.K. (2003). Introduction to plant tissue culture , Science Publishers, New Delhi. II Edition. 4. Chawla H.S. (2002). Introduction to Plant Biotechnology , Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, II Edition.
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Page 38 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER III MAJOR COURSE GLBP32 – GENETICS (For those admitted in June 2017 and later)
Contact hours per week 03 Total number of hours per semester 45 Number of credits 03
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: demonstrate the process of inheritance and interaction of genes in plants. CO2: analyze about linkage and crossing over and its significances. CO3: assess the structure, replication and role of nucleic acids. CO4: classify and detect the basis of mutations and its effects. CO5: apply statistical tools in life sciences.
CO-PO Mapping table (Course Articulation Matrix
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 3 - 3 - - - CO2 3 1 - - - - - CO3 9 3 - - - - - CO4 3 3 - - - - - CO5 9 9 3 3 - - 1 Weightage of 33 19 3 6 - - 1 the course Weighted percentage of Course 3.19 2.59 0.79 1.73 - - 0.88 contribution to POs
Unit – I (9 hrs) Mendel’s laws of heredity - law of dominance, segregation and independent assortment. Mono and dihybrid crosses. Modifications of 3:1 phenotypic ratio - incomplete dominance - Mirabilis jalapa and lethal gene action - Zea mays . Interaction of genes - dominant epistasis - Cucurbita pepo, recessive epistasis - length of petiole in Tobacco. Complementary genes - Lathyrus odoratus . Polygenic inheritance - skin colour in man.
Unit – II (9 hrs) Linkage - definition - types - complete linkage - Drosophila - incomplete linkage - Zea mays and its significances. Crossing over - mechanism - theories and significances.
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Page 39 of 159 Genetic code - general characters. Operon concept of gene - lac operon.
Unit – III (9 hrs) Nucleic acids - DNA - types - structure and replication - Watson and Crick model. RNA - types and structure. DNA as a genetic material - Griffith experiment and Hershey and Chase Experiment. Mechanism of sex determination in Melandrium album. Extra chromosomal inheritance in plants - Plastid inheritance.
Unit – IV (9 hrs) Mutation - definition and classification. CIB technique to detect mutation. Mutagens - physical and chemical mutagens and their mode of action. Chromosomal mutations - structural changes - addition - deletion - inversion and translocation. Numerical changes - euploidy and aneuploidy.
Unit – V (9 hrs) Measures of central tendency - mean - arithmetic mean (direct method only) - individual, discrete and continuous series - correcting incorrect mean, unknown value. Median and mode - individual, discrete and continuous series. Merits and demerits of mean, median and mode. Measure of dispersion - standard deviation (direct method only) and standard error.
Text Books: 1. Meyyan R.P. (reprint 2016). Genetics , Saras Publication, Nagercoil. VII Edition (Revised). Unit – I - Page No: 11-24, 32-54 and 82-84. Unit – II – Page.No: 88-97, 102-108, 329-332 and 381-383. Unit – III – Page No: 295-302, 306-318, 290-292, 270-271 and 200- 201. Unit – IV - Page.No: 228-245. 2. Ramakrishnan P. (2010). Biostatistics , Saras Publication, Nagercoil. III Edition (Revised). Unit – V – Page.No: 114-153 and 165-178.
Reference Books: 1. Gupta P.K. (1987). Genetics , Rastogi Publications, Meerut, II Edition. 2. Verma P.S. and V.K. Agarwal (1999). Genetics , S. Chand and Co., New Delhi, XIV Edition. 3. Veer Bala Rastogi, (1979). A Text Book of Genetics , Kedernath Ramnadh Publishers, Meerut, I Edition.
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Page 40 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER IV MAJOR COURSE GLBP41 - BIOCHEMISTRY (For those admitted in June 2017 and later)
Contact hours per week 05 Total number of hours per semester 75 Number of credits 05
Course outcomes (CO): On successful completion of the course, the learner should be able to CO1: outline the structure of atoms, nature of bondings and buffers. CO2: analyze the structure and properties of biomolecules. CO3: illustrate the classification of biomolecules. CO4: discuss the structure, properties, mechanism and role of enzymes. CO5: make use of the techniques and instruments for biochemical analysis.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 1 - - - 1 - - CO2 9 1 - 1 - - - CO3 9 3 3 1 - - - CO4 3 3 3 1 - - - CO5 3 3 3 3 1 - - Weightage of 25 10 9 6 2 - - the course Weighted 2.42 1.36 2.36 1.73 1.43 - - percentage of Course contribution to POs
Unit – I (15 hrs) Brief account of atoms - stable and unstable atom. Chemical bonds - bonding and properties of ionic - covalent - co-ordinate - anhydro bond and hydrogen bonds -Vander waal’s force. Molecular structure and properties of water. Buffer - types and its role.
Unit – II (15 hrs) Carbohydrates - classification. Structure and properties of monosaccharide - glucose, disaccharide - sucrose, polysaccharide - starch.
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Page 41 of 159 Lipids - classification, structure of triglyceride and fatty acids (saturated and unsaturated fatty acids) and properties - hydrolysis - saponification - rancidity - hydrogenation.
Unit – III (15 hrs) Amino acids - structure and general properties. Proteins - classification based on shape - composition and solubility. Structure of protein (primary, secondary, tertiary and quaternary). Properties of proteins. Vitamins - structure and properties of water soluble and fat soluble vitamins.
Unit – IV (15 hrs) Enzymes - classification - nomenclature. Structure and properties. Mechanism of enzyme action – lock and key model and induced fit model. Specificity of enzyme action, inhibition – reversible - competitive and noncompetitive inhibition. Biological role of enzymes.
Unit – V (15 hrs) Basic principles, techniques and applications of 1. pH meter 2. Colorimeter 3. UV – Vis spectrophotometer 4. Centrifuge 5. Chromatography – Paper and column Text Book: 1. Annie and N. Arumugam, (Reprint 2004). Biochemistry and Biotechniques , Saras publication, Nagercoil. Unit – II – Page.No: 39-56, 62-64, 68-70, 128-170. Unit- III _ Page No: 82-127. Unit- IV – Page No: 171-174, 180-190,195 -199. Unit -V – Page No: 203-226.
Reference Books: 1. Jain J.L. (2001). Fundamentals of Biochemistry , S.Chand and Co., New Delhi, VI Edition. Unit - I- Page No: 55-70, 19-24, 44-51. Unit - III - Page No: 959-1015. 2. Conn E.E., Stumpf, P.K., Brening, G and Doi, R,H. (1987). Outlines of Biochemistry, John Wiley and Sons, Singapore, V Edition. 3. Jeyaraman J., (Reprint 1985), Laboratory manual in Biochemistry, Wiley Eastern Ltd., Chennai. 4. Veerakumari L., (2009). Bioinstrumentation , MJP Publishers, Chennai. Unit – V – Page No: 309-312.
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Page 42 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER III & IV MAJOR COURSE GLBP4L - PRACTICAL II (Embryology, Plant tissue culture, Genetics and Biochemistry) (For those admitted in June 2017 and later) Contact hours per week : 03 + 03 Total number of hours per semester : 45 + 45 Number of credits 04
Course outcomes (CO): On successful completion of the course, the learner should be able to CO1 : demonstrate the preparation of culture medium and sterilization. CO2 : examine the germination of pollen grains and dissect out embryo . CO3 : analyze solution for a genetic problem with its cross. CO4 : interpret the role of several genes in the inheritance of character using statistical method. CO5 : make use of the biochemical techniques to detect and estimate the samples.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 3 9 3 3 - - CO2 3 1 - 1 - - - CO3 9 9 - 3 - - - CO4 3 9 - 3 - - - CO5 3 3 9 3 3 - - Weight age of 21 25 18 13 6 - - the course Weighted 2.03 3.41 4.72 3.76 4.29 - - percentage of Course contribution to POs
Embryology and Plant tissue culture • Effect of sucrose on pollen germination. • Any two stages of dicot embryo –Tridax. • Listing of pre- requisites of sterilization techniques. • Preparation of MS medium. • Surface sterilization procedure. • Initiation of callus. • Artificial seed preparation. Spotters • T.S. of anther- tetrad stage
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Page 43 of 159 • T.S. of mature anther microspore tetrad stage • Different types of ovules • L.S. of Monocot embryo • Adventive polyembryony • Autoclave • Laminar Air Flow chamber • Callus culture • Different cultures Genetics To work out simple genetic problems on monohybrid, dihybrid ratios and interaction of genes. To study polygenic inheritance of Quantitative traits in plants - length of leaves (Polyalthia ). Spotters • Watson and Crick model of DNA • Semi - conservative method of replication of DNA • Structure of tRNA • Griffith experiment • Hershey and Chase Experiment • Crossing over • CIB technique to detect mutation • Chromosomal mutations Biochemistry • Measurement of pH of a solution. • Titration of fatty acid. • Qualitative test for carbohydrate and protein. • Quantitative estimation of Protein by Lowry’s method • Qualitative test for amylase • Determination of complementary colour and verification of Beer’s law. • Paper chromatography – ascending and circular. Spotters • pH meter • Colorimeter • Centrifuge Submission of Record note book.
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Page 44 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI DEPARTMENT OF BOTANY SEMESTER III ALLIED COURSE GLBP3A – FUNDAMENTALS OF BIOCHEMISTRY (For those admitted in June 2017 and later) Contact hours per week 04 Total number of hours per semester 60 Number of Credits 04
Course outcomes (CO): On successful completion of the course, the learners should be able to CO1: explain the structure of atoms and nature of bondings and buffer. CO2: analyze the structure and functions of biomolecules. CO3: discuss about enzymology . CO4: assess the biochemical nature of nucleic acids, their role in living systems. CO5: make use of different techniques related to biochemistry.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 1 9 - - - - - CO2 1 9 - - - - - CO3 3 3 - - - - - CO4 3 1 3 - - - - CO5 1 3 1 1 - - - Weightage of 9 25 4 1 - - - the course
Unit – I (12 hrs) Brief account of atoms - atomic weight - number and stable and unstable atom. Chemical bonds - ionic - covalent - co-ordinate- hydrogen and anhydro bond (one example for each). Vander-waal’s force. Buffer and buffer action.
Unit – II (12 hrs) Carbohydrates - classification - structure and properties of monosaccharide - D- Glucose, disaccharide - sucrose, polysaccharide - starch. Lipids classification - structure and properties of triglyceride and fatty acids. Unit – III (12 hrs) Aminoacids - basic structure and properties. Proteins - classification, structure - primary - secondary - tertiary and quaternary structure. Nucleic acid – structure- chemical composition,- replication and its types. Type of DNA- biological significance and function. RNA – structure- chemical composition- types – mRNA- r RNA- t RNA and its function.
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Page 45 of 159 Unit – IV (12 hrs) Classification – nomenclature- properties- mechanism of enzyme action – lock and key and induced fit model- specificity of enzyme. Enzyme inhibition – competitive- non-competitive and allosteric inhibition. Biological role of enzymes.
Unit – V (12 hrs) Basic principles, techniques and applications of 1. pH meter 2. Colorimeter 3. Centrifuge 4. Chromatography – Ascending and circular Text Book: 1. Annie and N.Arumugam (2004). Biochemistry and Biotechniques , Saras publication, Nagercoil. Unit I- Page No: 8- 23 and 28-29. Unit II- Page No: 101-138, 152-167 and 179-194. Unit III-Page No: 235-244 and 299-328. Unit IV-Page No: 328-331, 340-354 and 365. Unit V-Page No: 691-702 and 709- 713.
Reference Books: 1. Jain J.L. (2001). Fundamentals of Biochemistry , S. Chand and Co., New Delhi, V Edition. Unit III-Page No: 246-252 and 262-271. 2. Jeyaraman J. (1985). Laboratory manual in Biochemistry, Wiley Eastern Ltd., Chennai. II Edition 3. Plummer D.T. (1979). An Introduction to practical Biochemistry , Tata Mc Graw Hill Publishing Co., Bombay, II Edition.
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Page 46 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI DEPARTMENT OF BOTANY SEMESTER IV ALLIED COURSE GLBP4A - MICROBIOLOGY AND BIOTECHNOLOGY (For those admitted in June 2017 and later) Contact hours per week 04 Total number of hours per semester 60 Number of Credits 04
Course outcomes: On successful completion of the course, the learners should be able to CO1: examine the contribution of microbiologists, general characteristics of microbes and their multiplication. CO2: analyze the involvement of microbes in agriculture. CO3: evaluate the role of microorganisms in industry. CO4: discuss about genetic engineering and their applications. CO5: explain the concepts and applications of plant tissue culture.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 1 3 - - - - - CO2 1 1 1 - - - - CO3 1 3 - - - - - CO4 1 3 - 1 - - - CO5 1 3 1 - - - - Weightage of 5 13 2 1 - - - the course
Unit – I (12 hrs) Contribution of Antonvan Leewenhock, Louis Pasteur. Bacteria – general characters- morphology of bacteria- colonization. Structure of bacteria – flagella- mesosome- endospore and reproduction – conjugation. Virus - size and shape- structure- reproduction of Bacteriophage – lytic cycle- lysogenic cycle.
Unit – II (12 hrs) Algal biofertilizer - mass cultivation- application and significance of BGA. Bacterial biofertilizer – mass cultivation and significance of Rhizobium. Microbial pesticide and its significance – Bacterial pesticide – Bacillus thuringiensis . Fungal pesticide – Trichoderma viride .
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Page 47 of 159 Unit – III (12 hrs) Biogas preparation – principle- mechanism- feeding material- KVIC model of biogas plant and its advantages. Industrial production of ethanol and its applications. Pasteurization technique.
Unit – IV (12 hrs) Genetic engineering – definition - enzymes involved in genetic engineering - restriction endonuclease- exonuclease – ligase - reverse transcriptase – S1 endonuclease - Taq DNA polymerase -. rDNA technology. Applications of genetic engineering in medicine, agriculture, industry and environment.
Unit – V (12hrs) Plant tissue culture – Principles – totipotency and cytodifferentiation. An outline procedure for plant production through tissue culture. Medium preparation – MS medium. Culture conditions. Explant and its preparation- callus culture- organogenesis and embryogenesis. Application of tissue culture in crop improvement.
Text Books: 1. Kumaresan V. (2010). Biotechnology , Saras Publication, Nagercoil. VI Edition. Unit II - 523-527, 529-530 and 556-558. Unit IV- 61-66, 74-84 and 179-185. Unit V – 435-453 and 466-469. 2. Mani A., Selvaraj A.M., Narayanan L. M., and N. Arumugam, (2013 Revised Edition). Microbiology, Saras Publication, Nagercoil, I Edition. Unit I -Page No: 6-7, 38-52, 57-62,131-134 and142-150. Unit III-Page No: 451,545-549 and 623-631.
Reference Books: 1. Dubey R.C. (2006), Text Book of Biotechnology, S. Chand & Co., New Delhi, Revised Edition. 2. Pelczar M.J., Kreig N.R. and E. C. S. Chan. (1998). Microbiology, McGraw Hill Book Company, V Edition.
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Page 48 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY SEMESTER – III & IV ALLIED COURSE GLBP4AL - ALLIED PRACTICAL (Fundamentals of Biochemistry, Microbiology and Biotechnology) (For those admitted in June 2017 and later) Contact hours per week : 02 + 02 Total number of hours per semester : 30 + 30 Number of credits 02
Course outcomes: On successful completion of the course, the learners should be able to CO1: demonstrate bacterial inoculation techniques. CO2: summarize the characterization of bacteria and their multiplication. CO3: detect and evaluate the biomolecules using analytical techniques. CO4: discover the ways to regenerate plantlets through tissue culture. CO5: Make use of various equipments to achieve sterilization techniques.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 1 3 - - - - - CO2 1 - 1 - - - - CO3 - 3 - - - - - CO4 1 3 1 - - - - CO5 1 3 - - - - - Weightage of 4 12 2 - - - - the course
Fundamentals of Biochemistry • Qualitative tests for carbohydrates • Qualitative tests for lipids • Qualitative tests for proteins • Measurement of pH • Determination of complementary colour • Verification of Beer’s law • Paper chromatography– circular and ascending
Microbiology and Biotechnology • Preparation of nutrient agar medium • Differential staining – Gram staining
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Page 49 of 159 • Pure culture technique – different streaking methods • Bacteriological testing of milk: dye reduction test – methylene blue • Preparation of MS medium Spotters • Colorimeter • Centrifuge • pH meter • pH electrode • Autoclave • Laminar air flow chamber • Structure of bacteria • Flagellation in bacteria • Endospore • Lytic cycle • Lysogenic cycle • Bacterial pesticides • KVIC Model of biogas Plant • Production of ethanol • Pasteurization of milk • rDNA technology • Callus culture Submission of record note book.
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Page 50 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY NON MAJOR ELECTIVE I (OPEN OPTION) SEMESTER – III GLBP3N – FRESHWATER AQUACULTURE (For those admitted in June 2017 and later)
Contact hours per week 02 Total number of hours per semester 30 Number of credits 02
Course outcomes ( CO): On successful completion of the course, the learners should be able to CO1: categorize the various types of freshwater aquaculture, cultivable species of carps and culture practices. CO2: construct and prepare fish culture ponds. CO3: outline nutrient management and to summarize predators, weeds and common diseases which occur in fish culture practices. CO4: manage soil and water quality parameters. CO5: adapt fishing crafts to harvest and evolve methodology to preserve the fishes by realizing the scope and need for aquaculture practices.
CO - PO Mapping Table ( Course Articulation Marks)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 9 - 3 - - - CO2 3 3 - 3 - - - CO3 3 1 - - - - - CO4 3 1 - - - - - CO5 1 - - 3 - - - Weightage of 19 14 - 9 - - - the course Weighted 1.84 1.91 - 2.6 - - - percentage of Course contribution to POs
Unit - I (10 hrs) Types of Freshwater aquaculture – Pond culture, reverine fish culture, Dam culture, lake culture, cold water fish culture. Cultivable species of carps - Catla, Rohu, Mrigal, common carp, cat fish and Murrels. Composite fish culture. Culture practices – Monoculture, Monosex culture, Poly culture, sewage fed fish culture, pen culture, cage culture, raft culture, Integrated culture : Animal husbandry cum aquaculture, Paddy cum fish culture.
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Page 51 of 159 Unit - II (10 hrs) Construction of Pond, Preparation of ponds, Nutrient management in fish culture ponds; Pond fertilization, supplementary feeding, stocking of fishes. Predators and weed control. Common Diseases in fishes.
Unit - III (10 hrs) Soil structure and quality, Management of water quality parameters - Temperature, pH, Dissolved oxygen, turbidity, carbon dioxide , hydrogen sulphide and plankton. Fish harvesting, Fishing Crafts, Fish preservation methods; salting, drying, smoking and freezing. Scope and need for Aquaculture practices.
Text Book: 1. Santhakumar G. A . (1993). Aquaculture , An Indian Perspective , JJ Publications. Unit - I P.No 5-35, 97 – 102, 86 – 96 and 106 – 108 . Unit – II P.No 48 -59 and 117. Unit – III P.No1 -4, 36 – 47 and 60 -66.
Reference Books: 1. Pullin R. and Shehaden Z. (Eds). (1980). Integrated agriculture-aquaculture farming systems. ICLARM. 2. Pillay T.V.R. (1991). Aquaculture and the environment . Fishing News Books / Blackwell Scientific. 3. Tamas C., Horvath L., Seagrave C. (1991). Carp and pond fish culture , Blackwell Scientific.
*Study material will be provided.
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Page 52 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY NON MAJOR ELECTIVE II (OPEN OPTION) SEMESTER - IV GLBP4N – SERICULTURE (For those admitted in June 2017 and later)
Contact hours per week 02 Total number of hours per semester 30 Number of credits 02
Course outcomes ( CO): On successful completion of the course, the learners should be able to CO1: outline the races and biology of silkworm and to list the causes and symptoms of diseases in silkworm. CO2: illustrate the basic design of the rearing house and to disinfect the rearing room and appliances. CO3: demonstrate the feeding frequency of silkworm and to evaluate the optimum environmental conditions for rearing and spinning. CO4: elaborate the strategies pertaining to harvest, sort, store, preserve, reel and market the cocoons and to relate world silk production and Central Silk Board. CO5: demonstrate mulberry cultivation and to relate causes and symptoms of diseases in mulberry plants.
CO - PO Mapping Table ( Course Articulation Marks)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 9 - 3 - - - CO2 3 3 - 3 - - - CO3 3 1 - - - - - CO4 3 1 - - - - - CO5 1 - - 3 - - - Weightage of 19 14 - 9 - - - the course Unit - I (10 hrs) Silkworm Biology;. Races of silkworm, Morphology of egg, larva, pupa and adult of Bombyx mori., Physiology of silk production , Life cycle of Bombyx mori , Causes and symptoms of diseases in silkworm. Rearing: basic design of rearing house, Rearing appliances-Disinfection of rearing house and rearing appliances Unit - II (10 hrs) Silkworm rearing: Frequency of feeding silk worm, optimum environmental conditions for rearing and Spinning, Harvesting: Time of harvesting of silk; sorting, storage, preservation, reeling and marketing. Budgeting. World Silk Production, Central Silk Board.
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Page 53 of 159 Unit - III (10hrs) Mulberry Cultivation: Irrigation, manuring and pruning . Nutrient value of leaves, Harvesting and storage of leaves, causes and symptoms of diseases in mulberry plants.
Text Book: 1. Ganga G. and Sulochana Chetty. 1991. An Introduction to Sericulture , Oxford & IBH Publishing Co. Pvt. Ltd. Unit - I P.No 48 – 61, 117 – 133. Unit - II P.No 91 – 113 Unit – III P. No 1-3 , 19 – 47, 115 – 116,135 – 155. Reference Books : 1. Narasingabba, M. N. (1988). Manual of silkworm and its production by Central Silk Board, Bangalore. 2. Narasaiah M.L. (2003). Problems and Prospects of Sericulture , Discovery Publishing House. 3. Venkatanarasaiah P. (2013). Sericulture , APH Publishing. *Study material will be provided.
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Page 54 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY DISCIPLINE SPECIFIC COURSE SEMESTER IV GLBP4DS – FOOD PROCESSING TECHNOLOGY (For those admitted in June 2017 and later) Contact hours per week 02 Total number of hours per semester 30 Number of credits 02 Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1 : explain the processing of cereals and pulses. CO2 : list out the advantages and disadvantages of food processing techniques. CO3 : analyse the steps involved in vegetable and fruit processing. CO4 : discuss the stages of meat, fish and poultry processing. CO5 : list out the significance of defeathering and deheading in poultry and fish processing.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 ------CO2 3 3 3 1 1 - - CO3 9 3 3 3 - - - CO4 3 - 9 - 3 - - CO5 3 - 3 - - - - Weightage of 21 6 18 4 4 - - the course Weighted 1.84 0.41 4.72 1.16 5.0 - - percentage of Course contribution to POs
Unit –I (10 hrs) Introduction to food processing. Processing on the Quality of foods - Types of processing - home scale, micro-scale and small scale. Food processing through cooking methods - double steaming - grill cooking - carbonation and blanching. Cereals and Pulses processing. Rice - harvesting - milling - parboiling advantages and disadvantages. Wheat – milling process. Pulses – puffing – flaking – milling.
Unit –II (10 hrs) Vegetable and Fruit processing technologies - harvesting and pre-processing- reception- temporary storage before processing- washing - sorting – trimming and peeling - heat blanching - acid treatment.
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Page 55 of 159
Unit –III (10 hrs) Meat and Poultry processing – slaughter house – process - curing - cuts and grades. Poultry – unloading bay - slaughter room – bleeding – scalding – defeathering – washing – chilling – draining – freezing. Fish processing – stunning of fish – grading – slime removal – scaling – washing – deheading – gutting – filleting – skinning – meat and borne separation.
Text Books: 1. M.K.Singh. (Reprint 2011). Food Processing . Discovery Publishing House, Sachin Printers, New Delhi. Unit -I - Page No : 45-51,57-58. Unit -III- Page No :33-36,41-44. 2. Sathya Prakash Sinha. (2009). Principles of Food Processing , Adhyayan Publishers and Distributors, New Delhi. Unit -I - Page No : 1-9. Unit -II- Page No : 21-29,61-68 Unit -III - Page No : 124-139 3. Srilakshmi B. (2007). Food Science , New age international publishers, IV Edition, New Delhi. Unit I : 31-34,37-40.
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Page 56 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI . DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY MAJOR COURSE SEMESTER V GLBP51 –TAXONOMY OF ANGIOSPERMS (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: explain the morphology of a plant in technical terms. CO2: analyse the importance of herbarium, binomial nomenclature and rules and recommendations of ICBN. CO3: identify the basis of systems of classification with its merits and demerits. CO4: conclude the characters and economic importance of different angiospermic families. CO5: compare the vegetative and floral characters of angiospermic families.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 9 - 3 - - - CO2 3 3 1 - - - - CO3 3 ------CO4 9 3 3 - - - 1 CO5 9 9 - 3 - - - Weightage of 33 24 4 6 - - 1 the course Weighted percentage of Course 3.19 3.27 1.05 1.73 - - 0.88 contribution to POs
Unit – I (18 hrs) Morphology Root system – types of root and its modifications. Shoot system – aerial and underground stem and its modifications. Leaves – simple and compound leaves. Phyllotaxy. Inflorescence – types - racemose, cymose, mixed and special types of inflorescence. Technical description of floral characters. Fruits – types - simple fleshy
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Page 57 of 159 fruits, simple dry fruits - dry dehiscent and dry indehiscent fruits, aggregate and multiple fruits. Unit – II (18 hrs) Binomial nomenclature – rules and recommendations of ICN - typification, author citation, principles of priority and nyms. Herbarium techniques – collection and pressing of specimens, drying, poisoning and mounting, Uses of herbarium. Study of the following systems of classification with merits and demerits. a. Artificial system – Linnaeus classification. b. Natural system - Bentham and Hooker’s classification. c. Phylogenetic system – APG IV system (Brief account).
Unit – III (18 hrs) Polypetalae Study of the following families with special reference to salient features and economic importance. 1. Annonaceae 5. Meliaceae 2. Brassicaceae 6. Caesalpiniceae 3. Sterculiaceae 7. Cucurbitaceae 4. Rutaceae
Unit – IV (18 hrs) Gamopetalae Study of the following families with special reference to salient features including floral characters and its economic importance. 8. Rubiaceae 11. Acanthaceae 9. Asclepiadaceae 12. Solanaceae 10. Apocynaceae 13. Lamiaceae
Unit – V Monochlamydeae and monocot (18 hrs) Study of the following families with special reference to salient features and economic importance. 14. Amaranthaceae 17. Liliaceae 15. Euphorbiaceae 18. Poaceae 16. Cannaceae
Text Books: 1. Narayanaswamy, R.V and Rao, K.N. (1976), Outlines of Botany , S.Viswanathan Printers and Publishers, Chennai. Unit I: Page No: 9-20, 26-40, 43-49, 71-80, 81-102 (technical terms alone) and 115- 123.
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Page 58 of 159 2. Sharma, O.P., (sixth reprint 2013), Plant Taxonomy, Tata McGraw- Hill, New Delhi, II Edition. Unit II : Page No: 79-82, 86-87, 55-59, 152-156, 21-22, 24-27. Unit III: Page No: 217-219, 243-245, 262-265, 276-280, 281-284, 302-305 and 329- 332. Unit IV: Page No: 349-352, 380-387, 397- 401 and 414 - 421. Unit V : Page No: 432-435, 445-450, 482-485, 490-493 and 518-523. Reference Books: 1. George H.M. Lawrence, (1969), Taxonomy of vascular plants , Oxford & IBH Publishing Company, New Delhi, II Edition. 2. Sambamurthy, A.V.S.S., (2005), Taxonomy of Angiosperms, I.K.International Pvt.Ltd, New Delhi. I Edition. 3. Pullaiah, T and Karuppusamy, S, (2018), Taxonomy of Angiosperms, Regency Publications, New Delhi. IV Edition. Unit II : APG IV system: 63-74.
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Page 59 of 159 THE STANDARD FIREWORKS RAJARAT NAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY MAJOR COURSE SEMESTER V GLBP5L - PRACTICAL III (Taxonomy of Angiosperms and Horticulture / Natural Resources) (For those admitted in June 2017 and later )
Contact hours per week : 03 + 03 Total number of hours per semester : 45 + 45 Number of credits 03
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: conclude the morphological features of a plant in technical terms. CO2: identify the plants and prepare herbarium. CO3: demonstrate the various plant propagation techniques. CO4: create bouquet and arrange flowers in different patterns. CO5: examine the beauty of nature.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 9 9 9 - - - CO2 9 9 3 3 - - - CO3 9 9 9 3 - - - CO4 1 9 1 3 9 - - CO5 3 3 3 3 3 - - Weight age of 31 39 25 21 12 - - the course Weighted 3.0 5.31 6.56 6.07 8.57 - - percentage of Course contribution to POs
Taxonomy of Angiosperms Morphology 1. Phyllotaxy Alternate, opposite, ternate, whorled and radical. 2. Types of inflorescence a. Racemose – raceme, panicle, spike, compound spike, catkin, corymb, umbel , compound spadix, head.
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Page 60 of 159 b. Cymose - solitary, simple, monochasial, dichasial and polychasial cyme . c. Mixed cyme – thyrsus and fascicle. d. Special types - verticillaster, hypanthodium and cyathium . 3. Types of Fruits a. Simple fleshy fruits - berry, drupe, hesperidium, pome and pepo. b. Simple dry fruits - Dry dehiscent – legume, follicle, lomentum, capsule – loculicidal, septicidal, porous, pyxidium , siliqua and schizocarp. Dry indehiscent - utricle, caryopsis, samara, nut . c. Aggregate and multiple fruits. Taxonomy • To refer angiospermic plants to their respective families giving reasons. • To describe the plant in technical terms. Draw labelled diagrams of the floral parts including the longitudinal section of the flower and to construct the floral diagram. • To identify at sight the angiospermic specimens from the local flora or from herbarium. 1. Annonaceae 10. Apocynaceae 2. Brassicaceae 11. Acanthaceae 3. Sterculiaceae 12. Solanaceae 4. Rutaceae 13. Lamiaceae 5. Meliaceae 14. Amaranthaceae 6. Caesalpinaceae 15. Euphorbiaceae 7. Cucurbitaceae 16. Cannaceae 8. Rubiaceae 17. Liliaceae 9. Asclepiadaceae 18. Poaceae
To submit a herbarium of 15 sheets correctly identified for external valuation. • Study tour / Field visit Horticulture Propagation Methods Soft wood cutting – Jasmine. Herbaceous cutting – Coleus . Hard wood cutting – Rose. Semi hard wood cutting – Hibiscus . Leaf cutting – Sansevieria. Layering – simple, compound and air layering. Grafting – whip, cleft, whip and tongue. Budding – T- Budding and patch budding. Spotters Horticultural implements
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Page 61 of 159 Layout of Kitchen Garden, Ornamental garden Indoor gardening - hanging basket, rockery, bonsai, indoor pots and terrarium. Flower vase arrangement and bouquet. Organic manures, oil cakes. Fertilizers – urea. NPK mixture. Biofertilizer - bacterial, algal. Vermicompost. Natural Resources 1. Social forestry, study of forest. 2. Energy- wind, solar, geothermal and hydrogen. 3. Bio gas plant - KVIC model. Field visit and study Tour. Submission of record note book.
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Page 62 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.S c. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY MAJOR COURSE SEMESTER VI GLBP61– PLANT PHYSIOLOGY (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: summarize the water relation in plants with respect to various physiological process. CO2: explain the various physiological processes in plants. CO3: determine the physical and biological nitrogen fixation in plants. CO4: analyze the growth of plants, role of mineral elements and effects of plant growth regulators. CO5: discuss about photoperiodism, vernalization and phytochromes in flowering plants.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 3 1 - - - - CO2 9 9 1 3 3 - - CO3 3 3 - 1 - - - CO4 9 9 3 1 3 - - CO5 3 3 - - - - - Weightage of 33 27 5 5 6 - - the course Weighted 3.19 3.68 1.31 1.45 4.29 - - percentage of Course contribution to POs
Unit – I (18 hrs) Absorption of water Imbibition, diffusion, osmosis, plant cell as osmotic system, plasmolysis - significance. Water absorption - Soil water, mechanism of water absorption – active and passive absorption. Factors affecting absorption of water - external and internal factors.
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Page 63 of 159 Ascent of sap - path of movement of sap, mechanism - theories on ascent of sap - vital theories, root pressure theory, physical force theories, capillary theory, imbibitional, atmospheric pressure theory, transpiration pull and cohesion theory. Mechanism of ascent of sap.
Unit – II (18 hrs) Transpiration - types – cuticular, stomatal and lenticular transpiration. Theories on mechanism of stomatal opening diurnal pattern., Steward’s theory and ion exchange theory. Factors affecting transpiration - significance of transpiration. Guttation. Role of mineral elements - functions and deficiency symptoms of macro and micronutrients. Mechanism of ion absorption - active and passive. Hydroponics.
Unit – III (18 hrs) Photosynthesis – photosynthetic apparatus – photosynthetic pigments, action and absorption spectra, photosynthetic unit. Mechanism of photosynthesis - Light reaction – cyclic and non cyclic photophosphorylation. Emerson enhancement effect, Dark reaction – C3, and C 4 cycles. Photorespiration - C2 pathway.
Unit – IV (18 hrs) Respiration. RQ of different substrates – carbohydrates, fats, protein, organic acids and RQ of succulents. Types- aerobic and anaerobic respiration. Mechanism of respiration - Glycolysis, Krebs cycle, electron transport system, pentose phosphate pathway and its significance. Growth – growth curve, measurement of growth, factors affecting growth. Plant growth regulators- physiological role of auxins, GA and kinetin.
Unit – V (18 hrs) Nitrogen fixation- physical and biological; Mechanism of biological nitrogen fixation- symbiotic- Rhizobium , asymbiotic- Clostridium . Photoperiodism – short day, long day, day neutral plant and photoperiodic induction. Role of phytochromes in flowering. Biological Clock – circadian rhythm.
Text Book: 1. Pandey, S.N. and B.K.Sinha, (1988), Plant Physiology , Vani Educational Books, New Delhi, III Edition. Unit I - Page No: 67-75, 80-89 Unit II - Page No: 80-102, 104-119 and121-134. Unit III – Page No: 202 – 260. Unit IV - Page No: 301 – 338,422-455 Unit V- Page No: 378-383,466-468,549-554 Reference Books: 1. Bidwell, R.G. S., (1986), Plant Physiology , Macmillan Publishing Co., New York, I Edition. 2. Jain, V.K., (1990), Fundamentals of plant physiology , S. Chand & Co., New Delhi, III Edition. 3. Ray Noggle G. and George J., Frits, (1982), Introductory Plant Physiology , Prentice Hall of India Pvt. Ltd., New Delhi, III Edition.
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Page 64 of 159 THE STANDARD FIREWOR KS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY MAJOR COURSE SEMESTER VI GLBP62 – BIOTECHNOLOGY (For those admitted in June 2017 and later)
Contact hours per week 05 Total number of hours per semester 75 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: analyze the role of enzymes and vectors in gene manipulation techniques. CO2: illustrate the aspects of DNA cloning strategies and its applications. CO3: discuss the role of microbes in biomining and in the production of vaccines and toxoids. CO4: examine the fermentation tools and techniques involved in the production of commercially valuable products. CO5: explain the mass production and applications of single cell protein, biofertilizers and microbial pesticide.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 3 - - - - - CO2 9 3 - - - - - CO3 9 9 1 1 - - - CO4 9 9 3 1 1 - - CO5 3 3 3 1 - - Weight age of 33 27 7 2 2 - - the course Weighted 3.19 3.68 1.84 0.58 1.43 - - percentage of Course contribution to POs
Unit – I (15 hrs) Genetic engineering – definition and principles. Tools of genetic engineering - restriction enzymes, reverse transcriptase, DNA polymerase, ligases, S1 nuclease and alkaline phosphatase. Vectors - general characters of plasmid, pBR 322, Ti plasmid, cosmids, phagemids and phasmids.
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Page 65 of 159 Unit – II (15 hrs) Cloning strategies – basic methods, rDNA technology - isolation of DNA - shotgun method - obtaining DNA from mRNA - hybridization method - reverse transcriptase method. Genomic library, cDNA library, chromosome walking and jumping. Applications of genetic engineering – Transgenic plants, GMOs – beneficial and harmful effects in health care products and Agriculture.
Unit – III (15 hrs) Blotting techniques - agarose gel electrophoresis, SDS-PAGE. Southern, Northern and Western blotting - procedure and application, DNA finger printing and its uses. Single cell protein – Spirulina – mass cultivation, industrial products and its applications.
Unit – IV (15 hrs) Fermentation technology – types- solid state fermentation, bioreactors- structure of fermentor, selection and culture of industrial micro-organisms and its characteristics. Commercial production of citric acid, ethanol, penicillin. Vaccines and toxoids. Biomining – bioleaching, removal of metals from water, microbial enhancement of oil recovery.
Unit – V (15 hrs) Algal biofertilizer - mass cultivation and significance of BGA. Bacterial biofertilizer – mass cultivation and significance of Rhizobium. Fungal biofertilizers – mycorrhiza. Fungal biocontrol agent – Trichoderma . Microbial pesticide – bacterial and viral pesticides.
Text Books: 1. Kumaresan V. (Reprint 2010), Biotechnology , Saras Publication, Nagercoil. VI Edition. Unit I - Page No: 64-89, 115-122 and 134-147. Unit II - Page No: 201-2114 and 66-72. Unit III - Page No: 14-19, 220-227, 306-313 and 570-583. Unit IV - Page No: 670-672, 677-678 and 682-690. Unit V - Page No: 516-520, 523-526, 536-539 and 556-561. 2. Patel A.H. (Reprint 2008), Industrial microbiology, Macmillan India ltd., Ahamedabed. Unit IV – Page No: 124-130, 145-150 and 112-118. 3. Powar C.B., Daginawala H.F. (Reprint 1990), General microbiology (Volume II) , Himalaya Publishing House. Bombay. Unit II - Page No: 138-143. Reference Books: 1. Dubey.R.C. (Reprint 2002), A text book of biotechnology . S.Chand and Company Ltd., New Delhi. IV Edition. 2. Satyanarayana.U. (Reprint 2010), Biotechnology . Books and allied Pvt. Ltd., Kolkata. I Edition.
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Page 66 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY MAJOR COURSE SEMESTER VI GLBP6L1 - PRACTICAL IV (Plant Physiology and Biotechnology) (For those admitted in June 2017 and later)
Contact hours per week : 03 + 03 Total number of hours per semester : 45 +45 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: evaluate the rate of photosynthesis under various conditions. CO2: analyse the effect of temperature and alcohol in permeability of cell. CO3: estimate chlorophyll content, imbibition rate and stomatal index. CO4: determine the effect of biofertilizers on growth. CO5: demonstrate various experiments related to biotechnology.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 9 9 3 3 - - CO2 3 9 9 3 3 - - CO3 9 9 3 3 1 - - CO4 3 9 9 1 3 - - CO5 9 3 3 3 3 - - Weight age of 33 39 33 13 13 - - the course Weighted 3.19 5.31 8.66 3.76 9.29 - - percentage of Course contribution to POs
Plant Physiology Experiments 1. Determination of Osmotic pressure – Plasmolytic method 2. Rate of Photosynthesis under different light conditions 3. Rate of Photosynthesis – Hydrilla experiments of Willmont’s bubble method using different colour filters.
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Page 67 of 159 4. Rate of Photosynthesis in different concentrations of Sodium bicarbonate (Bubble method) 5. Effect of temperature on cell permeability. 6. Effect of alcohol on cell permeability. 7. Imbibition - direct weight method. 8. Determination of stomatal index. 9. Determination of chlorophyll pigment. Experimental setups – Demonstration Only Retentivity of soil Capillarity of soil Adsorptive power of soil Potato osmoscope Varigated leaf experiment Continuity of intracellular spaces Suction due to transpiration Comparsion between cuticular and stomatal transpiration Ganong’s potometer Foliar transpiration – Bell jar experiment Ganong’s light screen experiment Determination of fermentation –Kuhne’s tube Ganong’s respiroscope Evolution of heat during respiration Geotropism, hydrotropism and phototropism Spotters Red drop and Emerson enhancement effect Transpiration and absorption ratio Sigmoid curve Auxanometer IAA NAA 2,4,D Cytokinin Gibberellin
Biotechnology I. Experiments 1. Isolation of DNA from plant cells 2. Isolation of plasmid DNA. 3. Analysis of DNA by agarose gel electrophoresis. 4. Isolation and identification of microbes present in soil samples. 5. Shelf life of biofertilizers in carrier based culture. i. Rhizobium , Azotobacter , Bacillus thruingiensis (Bt)
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Page 68 of 159 ii. Mass production of biofertilizers. iii. Measurement of bacterial growth by turbidity method. iv. Comparative study of treated and untreated biofertilizers in terms of root, shoot length, number of nodules. II. Demonstration only SDS-PAGE electrophoresis III. Spotters 1. pBR 322 2. Ti plasmid 3. Reverse transcriptase 4. DNA polymerase 5. rDNA technology 6. Southern, Western and Northern blotting techniques 7. cDNA library 8. DNA finger printing 9. Insect resistant transgenic plant 10. Fermentor – ethanol / citric acid / penicillin production.
Submission of record note book.
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Page 69 of 159 THE STANDARD FIREWORKS RAJARAT NAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY MAJOR COURSE SEMESTER VI GLBP6L2 - PRACTICAL V (Microbiology and plant pathology / Herbal Medicine/ Ecology and Biodiversity / Economic Botany) (For those admitted in June 2017 and later)
Contact hours per week : 03 + 03 Total number of hours per semester : 45 + 45 Number of credits 03
On successful completion of the course, the learners should be able to CO1: analyse the nature of vegetation by quadrat method. CO2: explain the anatomy of various groups of plants. CO3: demonstrate the preparation, sterilization and culturing of microbes. CO4: classify the group of microorganisms based on staining. CO5: inspect the role of plants in economy.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 9 1 1 3 - - CO2 3 9 3 1 - - - CO3 9 3 9 1 3 - - CO4 3 9 3 3 - - - CO5 3 3 3 3 - - - Weightage of 21 33 19 9 6 - - the course Weighted 2.03 4.5 4.99 2.6 4.29 - - percentage of Course contribution to POs
Microbiology and plant pathology Preparation of culture media – nutrient agar and nutrient broth. Culture techniques – serial dilution technique. Plating techniques –streak plate, pour plate and spread plate. Slant and stab culture. Bacterial staining – simple and Gram staining Fungal staining. Determination of bacteria by coliform test. Biochemical test for identification of bacteria – indole, methyl red, Voges- Proskauer, citrate utilization, urease, catalase, TSI agar. Enumeration of bacteria – Haemocytometer. Bacterial movement - Hanging drop technique.
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Page 70 of 159 Micropreparation of Cercospora / Albugo Spotters Ultra structure of bacterium. Shapes of bacteria. Types of flagella. Endospore. Reproduction in bacteria - binary fission, budding, fragmentation, conjugation. Structure of T 4 bacteriophage. Lytic cycle, Lysogenic cycle. Growth curve. Colony counter. Trickling filter. Oxidation pond. Symptoms of plant diseases - smut, black rust, chlorosis, necrosis Bunchy top of banana Red rot of sugarcane Citrus canker Herbal Medicine To identify the medicinal plants with reference to botanical name, family, morphology of the useful parts and their uses.
• Aloe vera • Mentha arvensis • Curcuma longa • Gymnema sylvestre • Hibiscus rosa-sinensis • Lawsonia inermis • Ocimum sanctum • Phyllanthus emblica • Solanum trilobatum • Aerva lanata
Study of the following drug plants with special reference to the botanical name, family, morphology of the useful part and uses.
Drugs obtained from root - Indian Sarsaparilla and Asafoetida. Drugs obtained from bark - Cinnamon. Drugs obtained from stem – Ephedrine. Drugs obtained from leaves - Digitalis. Drugs obtained from flowers – Saffron. Drugs obtained from fruits - Coriander, Pepper and Myrobalan. Drugs obtained from seeds – Fenugreek.
Industrial visit. Economic Botany Spotters Study of economic produces with special reference to the botanical name, family, morphology of the useful part and the uses of the following crops. • Cereals: wheat, ragi, sorghum.
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Page 71 of 159 • Pulses : black gram, bengal gram, soybean. • Fruits : pine apple, pomegranate, grapes. • Nuts : badam, cashew nut, walnut. • Spices and Condiments: Pepper, clove, cardamom. • Resins and Gums: canada balsam, turpentine. • Dyes : haematoxylin and indigo • Fibre : jute, cotton. • Latex : gutta percha and rubber. • Tannin : wattle bark and myrobalan. • Wood and cork: teak wood, sandal wood and cork. Ecology and Biodiversity 1. Spotters Ecosystem- pond, terrestrial and forest. Inverted pyramid of biomass in pond ecosystem Pyramid of biomass in a grass land ecosystem Pyramid of energy in forest ecosystem Food chain in grass land ecosystem Food web in grass land ecosystem Energy flow. Hydrophytes - Hydrilla, Pistia, Eichhornia, Nelumbo, Nymphaea and Marsilea, Xerophytes–Aloevera , Agave , Sansevieria , Euphorbia splendens Opuntia, Muehlenbeckia, Ruscus, Asparagus, Casuarina, Acacia, Parkinsonia, Zizyphus jujuba, Calotropis, Pinus needle. Halophytes- Rhizophora , Pneumatophores , Vivipary . Pollution – air, water, land, radioactive and noise. Rain water harvesting. Biogeochemical cycle-carbon, nitrogen, sulphur and water. 2. Sectioning T.S.of Hydrilla stem T.S.of Nelumbium petiole T.S of Casuarina cladode T.S of Nerium leaf T.S. of Parkinsonia phyllode
3. Quadrat study in different places.
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Page 72 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY MAJOR ELECTIVE COURSE SEMESTER V GLBP5E1 – MICROBIOLOGY AND PLANT PATHOLOGY (For those admitted in June 2017 and later)
Contact hours per week 05 Total number of hours per semester 75 Number of credits 05
On successful completion of the course, the learners should be able to CO1: recall the contribution of microbiologists. CO2: examine the characteristics and multiplication of microbes. CO3: acquire the knowledge of bacterial inoculation techniques. CO4: identify plant diseases and their remediation. CO5: analyze the microbiology of waste water and its implications.
CO-PO Mapping table (Course Articulation Matrix)
POs PO1 PO2 PO3 PO4 PO5 PO6 PO7
COs CO1 3 - - 3 - - - CO2 9 3 - - - - - CO3 3 9 3 - - - - CO4 3 1 3 - - - CO5 9 3 1 3 - - - Weightage of 27 15 5 9 - - - the course Weighted 2.61 2.04 1.39 2.6 - - - percentage of Course contribution to POs
Unit – I (15 hrs) Contribution of Microbiologists – Anton van Leeuwenhoek, Louis Pasteur and Robert Koch Postulates. Bergey’s manual of bacterial classification. Ultra structure of Bacteria- capsule, cell wall, mesosomes, nucleoid, flagella, pili and endospore. Ultra structure of Bacteriophage - reproduction – lytic and lysogenic cycle.
Unit – II (15 hrs) Microbial growth –reproduction methods - binary fission, budding, fragmentation, conjugation. Growth rate-generation time - Growth curve of bacteria – measurement of growth – cell number, cell mass. Growth in a batch culture, factors affecting growth – oxygen, temperature, pH. Nutritional types of bacteria – autotrophs – photoautotrophs – chemoautotrophs – heterotrophs – photoheterotrophs- chemoheterotrophs - saprotrophs
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Page 73 of 159 Unit – III (15 hrs) Culture media – nutrient agar and broth, PDA medium, Inoculation and Incubation. Isolation and purification technique -– serial dilution, plating - streak plate, pour plate and spread plate. Slant and stab cultures. Gram’s staining –simple, differential staining techniques. Biochemical test for identification of bacteria – indole, methyl red, voges-proskauer, citrate utilization, urease, catalase, TSI agar.
Unit – IV (15 hrs) Microbiology of domestic water – methods of purification of potable water- sedimentation, filtration and disinfection. Determination of sanitary quality of water. Sewage water – microbial characteristics.Wastewater treatment – primary, secondary and tertiary treatment. Determination of coliform bacteria – presumptive test, confirmative test, completed test.
Unit – V (15 hrs) Plant diseases –bacterial, fungal and viral symptoms, control and preventive measures. Causative organism, symptoms, etiology and control measures of the following diseases: Bacterial - Citrus canker Viral - Bunchy top of banana Fungal - Tikka disease of groundnut White rust of Albugo
Text Books: 1. Mani, A., Selvaraj A.M., Narayanan L. M., and N. Arumugam, (2011), Microbiology, Saras Publication, Nagerkoil, Revised Edition. Unit I – Page No: 5-7, 24-25,38-53 and142-148. Unit II –Page No: 57-62, and 159- 174 and 53-55. Unit III- Page No: 174-180,188-202,274-280 and 734-735. Unit IV - Page No: 481- 502. 2. Moshrafuddin Ahmed (2006), Applied Microbiology , MJP Publication, I Edition. Unit V- Page.No: 305-307, 330-331 and 318-319. Reference Books: 1. Rajeshwar Reddy K. (Reprint 2013). General Microbiology, New Age International Publishers, New Delhi. Unit III- Page No: 140-144. 2. Pelczar, M.J., Kreig, N.R. and E.C.S.Chan, (1998), Microbiology, McGraw Hill Book Company, V Edition.
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Page 74 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER V ELECTIVE COURSE GLBP5E2 - HORTICULTURE (For those admitted in June 2017 and later)
Contact hours per week 05 Total number of hours per semester 75 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: analyse the importance of horticulture, its tools and divisions. CO2: explain various methods of propagation and techniques practiced in horticulture. CO3: plan various types of garden. CO4: summarize the role of fruit and flowers in economy. CO5: discuss the importance of irrigation and manure.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 1 1 - - - - - CO2 9 9 9 3 1 - - CO3 9 3 3 1 - - - CO4 3 3 3 3 3 - CO5 9 3 3 - - - - Weightage of 31 19 18 7 4 - - the course Weighted 3.0 2.59 4.72 2.02 2.86 - - percentage of Course contribution to POs
Unit – I (15 hrs) Importance and divisions of Horticulture – pomology, olericulture, floriculture, spices and plantation crops, aromatic and medicinal plants. Garden tools and implements. Methods of propagation – seedage. Layerage –techniques, ground and air layering. Cuttage – root, stem, and leaf cutting. Graftage – types of grafting. Budding- methods. Transplantation- methods of transplantation.
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Page 75 of 159 Unit – II (15 hrs) Gardening - importance of gardening, designing, garden components - landscape gardening, land scaping, plant types and arrangements. – layout of garden – lawn - lawn making - seed, turfing, turf plastering, dibbing roots, plants for lawn making. Design and composition of ornamental gardening. Indoor gardening - topiary, terrarium, rockery, bonsai and hanging baskets.
Unit – III (15 hrs) Green House – plastic green house, net houses, polythene green house, list of green house plants, maintenance and its application. Training – details of training- height of head, number and distribution of scaffold limbs. Methods of leader training - open centre, central leader and modified leader. Pruning – season of pruning, special techniques – ringing, notching, smudging, de-blossoming and thinning- hand and chemical thinning.
Unit – IV (15 hrs) Planning and layout of orchard - vertical, alternate row planting pattern. Fruit drop – first, second, third drop, pre-harvest drop, causes and prevention. Fruit cultivation – Guava. Floriculture – Jasmine. Cut flowers - packages for export and significance. Dry flower arrangement - materials for dry arrangement, preservation of plant materials, design for dry arrangement, colour in dry arrangement, bases. Bouquet making.
Unit – V (15 hrs) Kitchen garden - selection of site, principle, layout, model kitchen garden and the cropping arrangement. Manures - organic – compost, vermi compost. Inorganic manures – NPK, urea, super phosphate, potash and mixed fertilizer. Manuring - time and method of application. Irrigation- types - surface irrigation - flooding, check, basins, ring, bed and furrow. Sub surface irrigation, special irrigation methods - overhead irrigation, drip irrigation. Text Book: Kumar,N., Introduction to Horticulture , Rohini agency, Nagercoil, V Edition, 2010. Unit – I – Page No: 4-12,148-176 and 363-364. Unit - II - Page No: 320-329 and 330-333. Unit – III – Page No: 44-47 and 74-90. Unit – IV – Page No: 97-101, 258-265, 373-377 and 406-410. Unit – V – Page No: 419-421, 52-60, 330-333 and 61-67. Reference Books: Adriance ,W. & R.Brison. Propagation of horticultural plants , Tata Mc Graw.Hill Publishing company Ltd, NewDelhi, II Edition, 1979. Unit – I - Page No: 260-270 Bose, K., Garden Plants. Oxford & IBH Publishing Co., Bombay, 1980. Manibhushan Rao, K., Text book of Horticulture , Macmillan India Ltd, NewDelhi, I Edition, 1991. Unit -I - Page No: 264-265. Gary L.McDaniel, Ornamental Horticulture, Second edition, Reston publishing, Reston, Virginia.
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Page 76 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER V ELECTIVE COURSE GLBP5E3 - NATURAL RESOURCES (For those admitted in June 2017 and later) Contact hours per week 05 Total number of hours per semester 75 Number of credits 05 Course Outcomes (CO): On successful completion of the course, the learner should be able to CO1: summarize the social forestry and forest protection. CO2: analyze about the conventional and non conventional energy resources. CO3: explain the biomass and bioconversion technology. CO4: elaborate the usage and over utilization of water resources. CO5: outline the uses, exploitation and conservation of mineral resources.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 - - 3 - - - CO2 9 3 - - - - - CO3 3 9 3 - - - - CO4 3 1 3 - - - CO5 9 3 1 3 - - - Weightage of 27 15 5 9 - - - the course Weighted 2.61 2.04 1.39 2.6 - - - percentage of Course contribution to POs
Unit – I (15 hrs) Forest resources Social forestry, forest protection - factors affecting forest protection internal and external dangers, afforestation, deforestation and its effect on environment.
Unit – II (15 hrs) Energy resources Energy – conventional – non-renewable forms – fossil fuel and its impact. Non conventional sources of energy – principle and applications of solar, wind, geothermal and hydrogen energy.
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Page 77 of 159 Unit – III (15 hrs) Energy resources Biomass – thermal conversion technology - combustion, pyrolysis, gasification and liquefaction. Bioconversion - biogas production – principle, feeding material and significance – biogas plant – KVIC model.
Unit – IV (15 hrs) Water resources Usage and over utilization of surface and ground water, floods, drought, dams, benefits and problems.
Unit – V (15 hrs) Mineral resources Uses and exploitation of major minerals of India, environmental effects of extracting and using mineral resources.Conservation of minerals resources.
Text Book: 1. Kaushik A., and C.P. Kaushik., (Reprint 2004), Perspectives in Environmental studies , New Age International Publishers, New Delhi, I Edition. Reference Books: 1. Koteswara Rao, M.V.R. (2004), Energy Resources – Conventional and Non Conventional, BS Publications, Hydrabad, I Edition. 2. Anjaneyulu, Y., (Reprint 2004), Introduction to Environmental Science, BS publications, Hyderabad. 3. Asthana, D.K. and Meera Asthana, (2006), A Text book of Environmental studies, S.chand & company Ltd, New Delhi, I Edition.
Study material will be provided.
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Page 78 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER V ELECTIVE COURSE GLBP5E4 – HERBAL MEDICINE (For those admitted in June 2017 and later) Contact hours per week 05 Total number of hours per semester 75 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: list out the medicinal plants and their cultivation process, active principles and mode of usage. CO2: evaluate the uses of drugs obtained from roots. CO3: examine the usage of drugs obtained from the stem and bark. CO4: discuss about the drugs obtained from leaves. CO5: summarize the drugs obtained from the flowers, fruits and seed.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 1 1 - - - - - CO2 9 9 9 3 1 - - CO3 9 3 3 1 - - - CO4 3 3 3 3 3 - CO5 9 3 3 - - - - Weightage of 31 19 18 7 4 - - the course Weighted 3.0 2.59 4.72 2.02 2.86 - - percentage of Course contribution to POs
Unit – I (15 hrs) Brief study of cultivation, active principles and mode of usage and medicinal value of the following medicinal plants. Aloe vera Mentha arvensis Curcuma longa Gymnema sylvestris Hibiscus rosa-sinensis
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Page 79 of 159 Unit – II (15 hrs) Brief study of cultivation, active principles and mode of usage and medicinal value of the following medicinal plants. Lawsonia inermis Ocimum sanctum Phyllanthus emblica Solanum trilobatum Aerva lanata
Unit – III (15 hrs) Study of the following drug plants with special reference to the Botanical name, family, morphology of the useful part and uses. Drugs obtained from root - Rauwolfia, Aconite, Celery, Belladona, Colocynth, Liquorice, Indian Sarassaparilla, Aswagandha and Asafoetida.
Unit – IV (15 hrs) Study of the following drug plants with special reference to the Botanical name, family, morphology of the useful part and uses. Drugs obtained from bark - cinnamon, quinine, asoka and wild orange. Drugs obtained from stem - ephedrine and catechu. Drugs obtained from leaves - life plant, Indian pennywort, digitalis, physic nut and tylophora.
Unit – V (15 hrs) Study of the following drug plants with special reference to the Botanical name, family, morphology of the useful part and uses. Drugs obtained from flowers - Saffron and Violet. Drugs obtained from fruits – Bael, Purging cassia, Coriander, Cumin, Funnel, Pepper and Myrobalan. Drugs obtained from seeds - Nux vomica, Carrot, Pungam and Fenugreek.
Reference Books: 1. Pandey, B.P., (1995), Economic Botany , S .Chand & Company Ltd, Delhi. IV Edition. 2. Purohit, S. S., and S.P. Viyas, (2004), Medicinal Plant Cultivation , AgroBios India, Jodhpur, I Edition. 3. Verma, V., (Reprint 1985), A text book of Economic Botany, Emkay Publication, Delhi.
Study material will be provided.
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Page 80 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER VI ELECTIVE COURSE GLBP6E2 – ECONOMIC BOTANY (For those admitted in June 2017 and later)
Contact hours per week 05 Total number of hours per semester 75 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: list out the economic produces with special reference to botanical name, family, morphology of the useful part and uses. CO2: discuss the commercial uses of pulses and cereals. CO3: analyse the core concepts of economic botany. CO4: identify the binomials and uses of fruits and nuts. CO5: distinguish the morphology of the useful part in the production resin, gum and tannin.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 3 - - - - - CO2 9 3 3 - 1 - - CO3 9 3 3 3 3 - - CO4 3 3 1 3 - - - CO5 9 3 3 - 3 - - Weight age of 33 15 13 6 7 - - the course Weighted 3.19 2.04 3.41 1.73 5.0 - - percentage of Course contribution to POs
Unit – I (15 hrs) Study of economic produces with special reference to the Botanical name, family, morphology, of the useful part and uses. Crops: Wheat, Ragi, Barley, Rye and Sorghum
Unit – II (15 hrs) Study of economic produces with special reference to the Botanical name, family, and morphology of the useful part and uses of the commercial products. Pulses: bengal gram, catan pea, pigeon pea, soybean, black gram and green gram.
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Page 81 of 159 Unit – III (15 hrs) Study of economic produces with special reference to the Botanical name, family, morphology of the useful part and uses. Fruits: mango, apple and pomegranate. Nuts: cashew nut and almond Spices and Condiments: pepper, clove and cardamom.
Unit – IV (15 hrs) Study of economic produces with special reference to the Botanical name, family, morphology of the useful part and uses. Resins and Gums: canadabalsam and turpentine. Dyes : haematoxylin and indigo
Unit – V (15 hrs) Study of economic produces with special reference to the Botanical name, family, morphology of the useful part and uses. Fibre : jute and cotton. Latex : rubber. Tannin : wattle bark and myrobalan. Wood and cork: teak wood and sandal wood.
Text Book: 1. Pandey, B.P., (1995), Economic Botany , S .Chand & Company Ltd, Delhi. IV Revised edition. Reference Books: 1. Albert F.Hill., (1952), Economic Botany, Tata Mc Graw Hill Publishing Co.Ltd, New Delhi, II Edition. 2. Das, P.C. (2009), Economic Botany , AITBS publishers, India, I Edition.
Study material will be provided.
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Page 82 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY B.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY ELECTIVE COURSE SEMESTER VI GLBP6E1 – ECOLOGY AND BIODIVERSITY (For those admitted in June 2017 and later)
Contact hours per week 05 Total number of hours per semester 75 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: explain the basics of ecology and ecosystem. CO2: analyse different groups of plants and their adaptations. CO3: discuss about major environmental pollutions. CO4: inspect the cycling of minerals and water. CO5: summarize biodiversity and its conservation.
CO-PO Mapping table (Course Articulation Matrix)
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 3 - - - - - CO2 9 3 3 - 1 - - CO3 9 3 3 3 3 - - CO4 3 3 1 3 - - - CO5 9 3 3 - 3 - - Weightage of 33 15 13 6 7 - - the course Weighted 3.19 2.04 3.41 1.73 5.0 - - percentage of Course contribution to POs
Unit – I (15 hrs) Introduction, scope of Ecology. Ecosystem - pond ecosystem. Ecosystem components – biotic - producers, consumers and decomposers and abiotic factors. Ecological pyramids- types- pyramid of numbers, biomass and energy - trophic levels - Food chain- types-grazing food chain, detritus food chain, food webs and energy flow- the first law of thermodynamics, the second law of thermodynamics. Unit – II (15 hrs) Study of the following groups with special reference to the morphological, anatomical and physiological adaptations.
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Page 83 of 159 (a) Hydrophytes – Classification - free-floating hydrophytes, submerged hydrophytes, rooted-floating hydrophytes, rooted submerged hydrophytes, rooted emergent hydrophytes (b) Xerophytes - Kinds of xerophytes - drought escaping plants, drought enduring plants, drought resistant plants. (c) Halophytes – Classification - salt escaping plants, salt evading plants, salt resisting plants, salt enduring plants. Unit – III (15 hrs) Environmental pollution - definition, pollutants - biodegradable and non - biodegradable pollutants, control measures. Source, effects and control measures of water, air, land, thermal, noise and radioactive pollutions.
Unit – IV (15 hrs) Biogeochemical cycles – phases of biogeochemical cycle, Types – flow of carbon, sulphur, phosphorus, nitrogen in biotic and abiotic system. Hydrocycle – Methods water harvesting and its significance.
Unit – V (15 hrs) Biodiversity - definition, components, various levels of biodiversity. Values of biodiversity. Endemism, Endangered plant and animal species. Conservation methods - In situ - wildlife sanctuaries, National park, biosphere reserves. Ex situ conservation-gene bank, seed bank, pollen bank, cryopreservation. Text Book: 1. Sharma, P.D., (2006), Environmental Biology , Rastogi Publications, New Delhi, VII edition. Unit I –Page No: 220-242 and 248-252. Unit II –Page No: 82-109. Unit III –Page No: 415-439, 455-465 and 470-483. 2. Arumugam. N, (2005), Concept of Ecology Saras publication, Nagercoil, I Edition. Unit I- Page No: 148 -182 and 191. Unit IV –Page No: 269 - 274. Unit V –Page No: 587-608. Reference Books: 1. Odum, E.P, (1978), Fundamentals of Ecology , W.B. Saunder Co., London, II Edition. 2. Saha T. K. (Reprint 2008), Ecology and Environmental Biology , Books and allied (p) Ltd., Kolkata.. Unit I- Page No: 1-10. Unit IV-Page No: 247-250. Unit V- Page No: 371-380. 3. Shukla R.S. and P.S. Chandel, (Reprint 2012), Plant Ecology and Soil Science , Chand and Co., Ltd., New Delhi, XII Edition. Unit II –Page No: 154 – 193.
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Page 84 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY SELF EMPLOYMENT COURSE SEMESTER - VI GLSE68– HERBAL COSMETICS (For those admitted in June 2017 and later)
Contact hours per week 02 Total number of hours per semester 30 Number of credits 02
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: summarize the knowledge on herbal cosmetics. CO2: relate the importance of skin and hair maintenance. CO3: apply the techniques of preservation and marketing of herbal products. CO4: analyse the effects of natural and synthetic cosmetics. CO5: make use of herbs for common ailments.
CO-PO Mapping table (Course Articulation Matrix)
POs PO1 PO2 PO3 PO4 PO5 PO6 PO7
COs CO1 9 ------CO2 1 1 - - - - CO3 3 3 - 9 - - 3 CO4 3 9 - - - - - CO5 3 1 - - - - 3 Weightage of 19 14 - 9 - - 6 the course
Unit – I (10 hrs) Anatomy, physiology and types of skin and hair – importance, maintenance and its benefits. Nutrient requirements for skin and hair care – sources. Deficiency symptoms - common ailments - herbal remedies.
Unit-II (10 hrs) Herbs to make cosmetics.Different forms of herbal cosmetics – preparation - facepack - face cream - shikakai powder - hair shampoo - hair oil - hair dye - hair colouring - crack ointment.
Unit-III (10 hrs) Harmful chemicals – synthetic cosmetics - impact on health - importance of traditional cosmetics. Preservation and marketing – herbal cosmetic products - scope for earning opportunities.
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Page 85 of 159 Reference Books:
1. Vimaladevi M.(I edition,2015) Textbook of Herbal Cosmetics , CBS Publishing, New Delhi,India.
2. Swarnlata Saraf, Shailendra Saraf,(3rd Edition, 2014) Cosmetics A Practical Manual (Cosmetics), PharmaMed Press, Hyderabad, India.
3. EIRI Board (Author), (2014 ) Herbal Cosmetics & Beauty Products with Formulations, Engineers India Research Institute, New Delhi, India.
4. Ranjan Magazine, 1ST edition (1 January 2011) Drugs and Cosmetics Formulations , CBS Publishing, New Delhi, India.
5. H Panda, (2015), Herbal Cosmetics Handbook , Asia Pacific Business Press, New Delhi,India.
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Page 86 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY SELF EMPLOYMENT COURSE SEMESTER – VI GLSE68L– HERBAL COSMETICS LAB (For those admitted in June 2017 and later)
Contact hours per week 02 Total number of hours per semester 30 Number of credits 02
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: recall the basic knowledge on natural products. CO2: recognize the basic medicinal herbs with scientific knowledge. CO3: discuss the effects of synthetic cosmetics. CO4: modify and preserve herbal cosmetics on their own. CO5: inspect the ways to market their own herbal products.
CO-PO Mapping table (Course Articulation Matrix) POs PO1 PO2 PO3 PO4 PO5 PO6 PO7
COs CO1 9 ------CO2 1 1 - - - - CO3 3 3 - 9 - - 3 CO4 3 9 - - - - - CO5 3 1 - - - - 3 Weightage of 19 14 - 9 - - 6 the course
List of practicals
Preparation of • Herbal bath powder • Herbal chooranum • Shikakai powder • Hair oil Demonstration • Face care • Hair care • Foot care • Herbal cosmetics
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Page 87 of 159
Page 88 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN (AUTONOMOUS), SIVAKASI – 626 123. (Affiliated to Madurai Kamaraj University, Re-accredited with A Grade by NAAC and College with Potential for Excellence by UGC) DEPARTMENT OF BOTANY M.Sc. DEGREE PROGRAMME IN BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY GUIDELINES FOR OUTCOME BASED EDUCATION WITH CHOICE BASED CREDIT SYSTEM (For those admitted in June 2017 and later) I. A. PREAMBLE The institutional vision is to emerge as a premier institution offering need-based, value conscious and career-oriented quality education to empower rural women with communicative competency and employment potential. With the advent of Autonomy in the year 2005, Choice Based Credit System (CBCS) is followed and it offers much flexibility to innovate and design the contents of each programme and align it with the institutional mission. Quality assurance developments in higher education have encouraged us to move towards outcomes-based approach to teaching, learning and assessment. Programme specifications define the students in terms of what they can do at the end of a programme or a particular level of study. This is a change from the more traditional approach where teachers tended to define courses in terms of what is taught, rather than what the student can do at the end of the course or programme. More directed and coherent curriculum, “more relevant” Graduates to industry and other stakeholders and Continuous Quality Improvement (CQI) are the benefits of OBE. A student-centered paradigm in higher education entails a shift from a more input- oriented curricular design based on the description of course content, to outcomes-based education in which the course content is developed in terms of learning outcomes. The implementation of Outcome Based Education with CBCS as per the UGC guidelines from the academic year 2019-2020 will definitely mark a paradigm shift from traditional education. B. OUTCOME BASED EDUCATION (OBE) FRAMEWORK
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Page 89 of 159 C. PROGRAMME EDUCATIONAL OBJECTIVES, PROGRAMME OUTCOMES AND PROGRAMME SPECIFIC OUTCOMES Programme Educational Objectives (PEOs): PEOs are broad statements that describe the career and professional achievements that the programme is preparing the graduates to achieve within the first few years after graduation. PEOs should be consistent with the mission of the Institution. PEO’s can be measured by a PO-PEO matrix. The PEO’s should evolve through constant feedback from alumnae, students, industry, management etc,. It is mandatory that each PEO should be mapped to atleast one of the POs. The Graduates will PEO1: exhibit a mastery of skills and knowledge at a level required for plant based industry or to be an eminent research scholar. PEO2: pursue research of significance in Botany or an interdisciplinary or creative project to solve the problems in thrust areas. PEO3: enhance the productivity of several economically important products/botanicals and thereby become a successful entrepreneur. PEO4: imbibe moral, ethical and professional values to preserve nature for a better and respectable life in the society. Programme Outcomes (PO): Programme Outcomes are narrower statements that describe what students are expected to know and be able to do upon the graduation. These relate to the skills, knowledge and behaviour that students acquire in their study through the programmes. PO1: Disciplinary knowledge Apply the knowledge of Arts, Science and Humanities to address fundamental and complex questions appropriate to their programmes. PO2: Critical thinking, Problem solving and Analytical reasoning Make use of appropriate knowledge and skills to identify, formulate, analyze and solve problems in order to reach substantiated conclusions. PO3: Research related skills and scientific reasoning Critically analyze research processes, products and practices with a view of strategic use of data in their field. PO4: Communication skills and Digital literacy Demonstrate skills in oral and written communication and make use of ICT in various learning ambience. PO5: Team work and Leadership quality Interact productively with people from diverse backgrounds as both leaders/mentors and team members with integrity and professionalism. PO6: Multicultural competence with Moral and ethical awareness Defend the society against gender and environmental issues with moral and ethical awareness. PO7: Self-directed and Life-long learning Formulate their own educational needs in a changing world in ways sufficient to maintain their competence and to allow them to contribute to the advancement of knowledge.
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Page 90 of 159 Programme Specific Outcomes (PSO): Programme Specific Outcomes denote what the students should be able to do at the time of graduation. They are programme specific. It is mandatory that each PO should be mapped to the respective PSO specified in the programme in order. By the completion of the M.Sc. Botany with specialization in Plant Biotechnology programme, the learners will be able to PSO1: demonstrate a systematic, extensive and coherent knowledge of plant sciences and its application with the use of established theories, principles and concepts of Botany. PSO2: apply their botanical knowledge and transferable skills to identify and analyze issues related to new/unfamiliar contexts and to solve it with well-defined solutions. PSO3: be competent on data collection and process of scientific documentation in areas related to specializations and current updates in the field of Botany. PSO4: make use of appropriate techniques, skills and modern ICT tools necessary to decipher knowledge related to life sciences. PSO5: demonstrate leadership and team workmanship in order to serve efficiently in institution, industry and society. PSO6: defend the environmental and professional issues with moral ethics. PSO7: involve in life-long learning and to adapt to the technological advancements in the emerging areas of Botany. BLOOM’S TAXONOMY: Bloom's Taxonomy was created in 1956 by an educational psychologist Dr. Benjamin Bloom in order to promote higher forms of thinking in education, such as analyzing and evaluating concepts, processes, procedures, and principles, rather than just remembering facts. It is most often used when designing educational, training, and learning processes.
The K-levels mentioned in the diagram are usually denoted as [k1] to [k6] respectively from the bottom.
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Page 91 of 159 Course Outcomes (CO):
Course Outcomes are narrower statements that describe what students are expected to know and be able to do at the end of each course. These relate to the skills, knowledge, and behaviour that students acquire in their study through the course. Each course comprises five COs and the keywords used to define COs are based on Bloom’s Taxonomy[k1] to [k6]. On successful completion of the course, the learners should be able to CO1: [k1] / [k2] CO2: [k3] CO3: [k4] CO4: [k5] CO5: [k6]
D. CO-PO &PO-PEO relationship:
E. CO – PO MAPPING OF COURSES: After CO statements are developed by the course in-charge, COs will be mapped with any possible POs based on the relationship exist between them. A CO must be mapped to atleast one PO. The PO’s which are not related to any of the COs in a particular course may be left blank. All the courses together must cover all the POs. The CO-PO matrix for a course is as shown below. The correlation between COs and PO can be defined by three levels using the Letter Grades H, M, L which denotes respectively High (H), Medium (M), Low (L) and ‘- ‘ for no correlation. The concept of Six Sigma is used for calculating weighted percentage of contribution of each course in attainment of respective POs. As per Six Sigma Tool- Cause and Effect Matrix, the weightage of H, M and L are 9, 3 and 1 respectively.
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Page 92 of 159 CO-PO Mapping table (Course Articulation Matrix)
POs PO1 PO2 PO3 PO4 PO5 PO6 PO7
COs CO1 CO2 CO3 CO4 CO5 Weightage of the course
Weighted percentage of Course contribution to POs
The levels of contribution are denoted by Grades and weightages H-High (9), M- Medium (3), L-Low (1) Weighted percentage of Contribution of the Course in attainment of PO1= Weightage of the course / Total weightage of all courses contributing PO1 computed based on correlation between COs and POs X 100 Programme Articulation Matrix (PAM):
Course Course Title PO1 PO2 PO3 PO4 PO5 PO6 PO7 Code
Total Weightage of all courses contributing to POs
PO-PEO Mapping Matrix:
PEOs PEO1 PEO2 PEO3 PEO4 POs PO1 X X X PO2 X X PO3 X X PO4 X X PO5 X X X PO6 X X PO7 X (Mark X to map a PO to a PEO)
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Page 93 of 159 F. MEASUREMENT OF ATTAINMENT OF DESIRED GOALS: Course Outcome (CO) is measured through the performance of students by the various assessment tools adopted for that particular course. Each evaluation tool is mapped to a particular verb in Bloom’s Taxonomy and further each verb is mapped to a particular CO. Once the Course Outcome is measured, the PO can be measured using a CO-PO matrix. Measurement of PO attainment shall be done by direct and indirect methods. Direct assessment method and indirect assessment method are considered for 80% and 20% weightages respectively. Target levels of attainment shall be fixed by the Course teacher and Heads of the respective departments. Direct assessments (rubric based) - Conventional assessment tools such as Term Test, Quiz, Seminar, Assignment and End Semester Examination. Indirect assessments – Course Survey, Graduate Exit Survey, Feedback from Alumnae, Employer and Parents. G. ASSESSMENT PROCESS:
Assessment is one or more processes carried out by the institution that identify, collect and prepare data to evaluate the achievement of course outcomes and programme outcomes. Assessment Process for CO Attainment: For the evaluation and assessment of CO’s and PO’s, rubrics are used. (i) CO Assessment Rubrics: Course Outcome is evaluated based on the performance of students in the Continuous Internal Assessments and in End Semester Examination of a course. Internal assessment contributes 25% and End Semester assessment contributes 75% to the total attainment of a CO.
(ii) CO Assessment Tools: The description of Assessment tools used for the evaluation of COs and POs is given below. Mode of Assessment Description Evaluation of Course Related POs Assessment Tool Outcomes Theory Courses- Internal Assessment (Weightage 25%) Theory- Three written The questions in the three Term Test examinations Term Tests, Quiz and are conducted Assignment are framed in and average such a way that they cover of best two is all the COs of respective considered course. PO1 to PO7
Assignment One The final attainment for Assignment is each CO under direct Direct given per assessment is calculated (Weightage course by taking average of the 80%) Quiz/Seminar One CO attainments from Term Quiz/Seminar Tests, Assignment and
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Page 94 of 159 is conducted Quiz. for each course Theory Courses- External Assessment(Weightage 75%) End Semester Examination It covers the entire PO1 to PO7 Examination at the end of syllabus of the course. It the course of would generally satisfy all 3- hour course outcomes for a duration particular course. The COs are evaluated based on the set attainment levels.
Practical Courses - Internal Assessment (Weightage 40%) Observation Day to day Lab exercises are planned PO1 to PO7 evaluation to cover all COs and CO Model Model Exam attainment is calculated. Practical is conducted Examination for each lab course. Practical Courses - External Assessment (Weightage 60%) End Semester Examination The final attainment for PO1 to PO7 Practical at the end of each CO under direct Examination the course of assessment is calculated 3- hour by taking average of the duration CO attainments in Observation, Model Practicals and End Semester Practical Examination Indirect Course This survey At the end of each PO1 to PO7 (Weightage Survey gives the course an exit survey is 20%) opinion of the collected from the students on students and Considered attainment of for the CO attainment Course under Indirect assessment Outcomes
(iii) CO Attainment: Direct CO Attainment: Course outcomes of all courses are assessed and the CO wise marks obtained by all the students are recorded for all the assessment tools mentioned above. The respective CO attainment level is evaluated based on set attainment rubrics. Attainment Levels of COs
Assessment Methods Attainment Levels Level 1 60% of students scoring more than average marks or set target marks in internal assessment tools Level 2 70% of students scoring more than average marks or set target marks in internal assessment tools
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Page 95 of 159 Internal Assessment Level 3 75% of students scoring more than average marks or set target marks in internal assessment tools
Level 1 60% of students scoring more than average marks or set target marks in End Semester Examination Level 2 70% of students scoring more than average marks End Semester Examination or set target marks in End Semester Examination Level 3 75% of students scoring more than average marks or set target marks in End Semester Examination
Target setting for Assessment method: For setting up the target of internal assessment tools, the average of last three tests must be taken into consideration and it should be kept as target. If the average marks are not available then current average can also be used as target. For setting up the target of End Semester Examination, the average of the last year examination shall be set as target. If the average marks are not available then current average can also be used as target. Formula for Attainment for each CO: Attainment = Percentage of students who have scored more than the target marks
➢ Internal Attainment is the average of attainments obtained using various internal assessment tools. ➢ For Theory Courses, Direct CO Attainment =25% of internal attainment + 75% of End Semester attainment ➢ For Practical Courses, Direct CO Attainment =40% of internal attainment + 60% of End Semester attainment Indirect CO Attainment: At the end of each course, an exit survey is collected from the students and it gives the opinion of the students on attainment of Course Outcomes. A questionnaire is designed to reflect the views of the students about the attainment of course outcomes. Overall CO Attainment = 80% of Direct CO Attainment + 20% of Indirect CO Attainment In each course, the level of attainment of each CO is compared with the predefined targets, if the target is not reached, the course teacher takes necessary steps for the improvement to reach the target. If the average attainment of a particular course for two consecutive years is greater than 80% of the maximum attainment value (i.e. 80% of 3 = 2.4), then for that particular course the current rubrics for attainment must be changed to analyze continuous improvement.
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Page 96 of 159 II. ASSESSMENT PROCESS FOR OVERALL PO ATTAINMENT
With the help of CO against PO mapping, the PO attainment is calculated. PO assessment is done by giving 80% weightage to direct assessment and 20% weightage to indirect assessment. Direct assessment is based on CO attainment, where 75% weightage is given to attainment through End Semester examination and 25% weightage is given to attainment through internal assessments. Indirect assessment is done through Graduate exit survey. PO Assessment Tools
Mode of Assessment Assessment Tool Description
Direct Attainment CO Assessment This is computed from the calculated CO (Weightage 80%) Attainment value for each Course Indirect Attainment Graduate Exit At the end of the programme, Graduate Exit (Weightage 20%) survey Survey is collected from the graduates and it gives the opinion of the graduates on attainment of Programme Outcomes Direct Attainment of POs for all Courses At the end of the each programme, the direct PO assessment is done from the CO attainment of all courses. The direct PO attainment for a particular course is determined from the attainment values obtained for each course outcome related to that PO and the CO-PO mapping values. For the evaluation and assessment of CO’s and PO’s, the same set of rubrics is used. Programme Articulation Matrix (PAM):
Course Course Title PO1 PO2 PO3 PO4 PO5 PO6 PO7 Code
Average Direct PO Attainment
Direct PO Attainment in %
Indirect Attainment of POs for all Courses
POs PO1 PO2 PO3 PO4 PO5 PO6 PO7
Graduate Exit survey Indirect PO Attainment
Indirect PO Attainment = 20% of PO Attainment from Graduate Exit survey Attainments of POs for all Courses
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Page 97 of 159
POs PO1 PO2 PO3 PO4 PO5 PO6 PO7
Direct Attainment (Weightage 80%) Indirect Attainment (Weightage 20%)
Overall PO Attainment
Overall PO Attainment = 80% of Direct PO Attainment + 20% of Indirect PO Attainment
III. ASSESSMENT PROCESS FOR PROGRAMME EDUCATIONAL OBJECTIVES The curriculum is designed so that all courses contribute to the achievement of PEOs. The marks secured by the students in these courses indicate the level of achievement of the PEOs. In addition, Alumnae survey, Placements of students and progression to higher studies also contribute to the attainment of PEOs.
Indicators Type of Data Assessment Assessment Responsible for Assessment collection Tool criteria entity Attainment frequency of PEO PO This is computed from the calculated PO PEO-1 Direct Assessment Attainment values for each Course PEO-2 Weightage PEO-3 70% PEO-4 Alumnae Once in a year, Alumnae Survey is PEO-1 Survey collected from the alumnae and it gives the PEO-2 Weightage opinion of the alumnae on attainment of PEO-3 10% Programme Outcomes and their PEO-4 achievements of in a Placement Number Once Placement PEO-1 Record students year cell PEO-2 Indirect Weightage Placed PEO-3 Weightage 10% PEO-4 30% Higher Number of Once in a Department PEO-1 Education students year PEO-2 Weightage opted for PEO-3 10% higher PEO-4 education
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Page 98 of 159 The attainment of the PEOs Direct Evaluation of Programme Outcomes (POs) of the concerned PEO
POs PEO1 PEO2 PEO3 PEO4 PO1 PO2 PO3 PO4 PO5 PO6 PO7 Average Direct PEO Attainment in % Total Direct PEO Attainment Indirect Attainment of PEOs
PEOs PEO1 PEO2 PEO3 PEO4
Alumnae Survey
Average PEO Attainment from Alumnae Survey
Indirect Attainment from placement
Indirect Attainment from higher studies
Indirect PEO Attainment = 10% attainment of Alumnae survey + 10% Attainment from placement+ 10 % Attainment from higher studies
Overall PEO Attainment = 70% of Direct PEO Attainment+ Indirect PEO Attainment Expected Level of Attainment for each of the Programme Educational Objectives PEO Level of Attainment Value >=70% Excellent Value > = 60 and value < 70 Very good Value > = 50 and value < 60 Good Value > = 40 and value < 50 Satisfactory Value < 40 Not Satisfactory
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Page 99 of 159 Level of PEO attainment
Graduation Overall PEO Attainment Whether Expected level of Batch PEO is achieved?
Process of Redefining the PEOs: The college has always been involving the key stake holders in collecting information and suggestions with regard to curriculum development and curriculum revision. Based on the information collected the objectives of the programme are defined, refined and are inscribed in the form of PEO’s. The level of attainment of PEO’s defined earlier will be analyzed and will identify the need for redefining PEOs. Based on identified changes in terms of curriculum, regulations and PEOs, the administrative system like BOS, Academic Council and Governing Body involve appropriate actions.
IV. Eligibility condition for admission:
As per the G.O(ID) No. 75, for admission to M.Sc. Botany with Specialization in Plant Biotechnology, a candidate must have passed a 3 years degree course in Botany / Plant Biology and Plant Biotechnology / Plant science (under the 10+2+3 pattern) recognized by the university as equivalent there to.
V. Duration of the Programme:
The duration of the programme is two academic years. Each Academic Year consists of two semesters. The duration of a semester is 90 working days.
VI. Attendance: The Rules regarding the attendance for regular classes for the candidates to appear for the End Semester examinations are framed as given below: a) Each student must put in a minimum attendance of 68 days (75% of 90 days per semester) so as to become eligible to appear for the End Semester examinations.
Shortage of attendance:
b) Those students with an attendance of 67 days and less but 59 days and above (65%) can be permitted to appear for the End Semester examination provided, they get the condonation certificate from the Principal stating the proper reasons for the absence, within 5 days after the last working day of the concerned class. The Certificate may be obtained from the office on payment of penalty as per Madurai Kamaraj University norms.
c) In case of attendance with 58 days and less but 45 days and (50%) above, the students cannot appear for the End Semester examination of that semester but can appear for the next End Semester examinations by obtaining special permission from the Principal providing necessary
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Page 100 of 159 documents supporting the reasons for absence on payment of penalty as per Madurai Kamaraj University norms.
d) Students with an attendance of 44 days and less should repeat the whole semester.
VII. Evaluation Procedure: Evaluation of each theory course will be 25% for CIA and 75% for End Semester examination. Evaluation of each Practical Course will be 40% for CIA and 60% for End Semester Examination. Project will be evaluated for 100% in the End Semester Examination. A mark statement will be issued to every student at the end of every semester.
VIII. Passing Minimum:
For a pass in each course a student should secure a minimum of 45% marks in the End Semester examinations and a minimum of 50% marks in aggregate (i.e. marks of CIA and End Semester examinations put together).The same rule is applicable for Dissertation/Project report and Viva – Voce.
Minimum credits to be earned for M.Sc Botany with specilization in Plant Biotechnology Programme is 90 credits.
IX. Eligibility condition for getting the Degree:
A Candidate undergoing the M.Sc. degree Programme in M.Sc. Botany with specilization in Plant Biotechnology will be eligible for the award of degree in M.Sc. Botany with specilization in Plant Biotechnology; if she completes the entire Programme and passes all the examinations prescribed for the Programme.
As per UGC guidelines, a student who is not able to complete the Programme within two years, may be allowed for 2 years period beyond the two years duration to clear the backlog to be qualified for the degree.
X. Classification of Successful Candidates: The successful candidates will be classified as per the details given in the table below: CGPA Grade Classification of Final Result 9.50000 – 10.00000 O+ 9.00000 – 9.49999 O
8.50000 – 8.99999 D++ 8.00000 – 8.49999 D+ First Class 7.50000 – 7.99999 D 7.00000 – 7.49999 A++ 6.50000 – 6.99999 A+ 6.00000 – 6.49999 A
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Page 101 of 159 5.50000 – 5.99999 B+ Second Class 5.00000 – 5.49999 B 4.50000 – 4.99999 C+ Third Class 4.00000 – 4.49999 C 0.00000 – 3.99999 U Re-appear XI. Award of Ranks:
Candidates who qualify themselves for the respective Degree Programme, passing all the examinations in the first attempt are eligible for ranking based on the CGPA.
For Each Major:-
Σi CiGi CUMULATIVE GRADE POINT AVERAGE [CGPA] =
Σi Ci Sum of the multiplica tion of grade points by the respective credits of the course cleared in the entire programme
CGPA = Sum of the credits of all the courses cleared in the programme
‘Ci’ = Credits earned for course i in any semester
‘Gi’= Grade point obtained for course i in any semester
‘Σi’= Summation of all courses cleared in a semester in the case of GPA and all courses cleared in all semesters in the case of CGPA. XII. Other Provisions: 1. In the Mark Statement, ‘AA’ will be marked against the courses for which the candidate was absent for the examination. 2. If a candidate is found indulging in malpractice, she will be expelled from the examination hall right away and debarred from appearing in all examinations of that particular semester. She can be allowed to take up examination only in the consecutive semester. 3. The courses she has already appeared during that semester will not be considered. 4. A student can appear for any number of arrear courses. 5. Repeat Examinations will be conducted for the final semester paper within a month after the publication of final semester results. 6. Revaluation is permitted.
XIII. Transitory Provisions: Students from other institutions will be granted year exemption alone and she has to appear and pass all the courses of all semesters under CBCS pattern in order to get the consolidated Statement of Marks/ Grade. Those students who have discontinued in the middle of the Programme may be admitted in the respective semester if they want to rejoin and complete the Programme, provided they had not got their transfer certificate.
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Page 102 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN (Autonomous), SIVAKASI DEPARTMENT OF BOTANY (PG PROGRAMME) Allotment of Hours and Credits (For those who have joined in June 2017 and later)
Subjects I II III IV Total
Semester Semester Semester Semester Credits
Core courses:
Paper I 6 (5) 6 (5) 6 (5) 6 (5)
Paper II 6 (4) 6 (4) 6 (4) 6 (5)
Paper III 6 (4) 6 (4) 6 (4) --
Lab 6 (4) 6 (4) 6 (4) 6 (4)
Project and Viva - - - 6 (5) Voce Total 24(17) 24(17) 24(17) 24 (19) 70
6 (5) 6 (5) 6 (5) 6 (5) 20 Electives: Total 30 (22) 30 (22) 30 (22) 30 (24) 90
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Page 103 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN (Autonomous), SIVAKASI. DEPARTMENT OF BOTANY (PG PROGRAMME) (For those who have joined in June 2017 and later)
Marks Allotted
Teaching Duration Course Course Title of the Paper Hours Per of Exam Code
Week Credits (Hours)
Total
Semester
Internal
External
HLBP11 Plant Diversity 6 5 3 25 75 100
HLBP12 Inheritance Biology 6 4 3 25 75 100 I HLBP13 Taxonomy of Angiosperms 6 4 3 25 75 100 HLBP1L Practical I 6 4 5 40 60 100 HLBP1E Elective I 6 5 3 25 75 100 HLBP21 Plant Anatomy and Embryology 6 5 3 25 75 100 HLBP22 Biochemistry 6 4 3 25 75 100
II HLBP23 Microbiology 6 4 3 25 75 100 HLBP2L Practical II 6 4 5 40 60 100 HLBP2E Elective II- Food Preservation 6 5 3 25 75 100 HLBP31 Cell and Molecular Biology 6 5 3 25 75 100 HLBP32 Environmental Microbiology 6 4 3 25 75 100 III HLBP33 Plant Physiology 6 4 3 25 75 100 HLBP3L Practical III 6 4 5 40 60 100 HLBP3E Elective III 6 5 3 25 75 100 HLBP41 Plant Ecology 6 5 3 25 75 100 HLBP42 Applied Biotechnology 6 5 3 25 75 100 IV HLBP4L Practical IV 6 4 3 25 75 100 HLBP4E Elective IV 6 5 3 25 75 100 HLBP4P Project and viva voce 6 5 - - 100 100 ELECIVES HLBP1E1 Bioinstrumentation 6 5 3 25 75 100 I HLBP1E2 Herbal Medicine 6 5 3 25 75 100 HLBP3E1 CSIR UGC-NET Preparatory course - 6 5 2 25 75 100 III Life Sciences HLBP3E2 Energy resources 6 5 3 25 75 100 HLBP4E1 Biophysics and Biostatistics 6 5 3 25 75 100 IV HLBP4E2 Microbial Genetics 6 5 3 25 75 100
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Page 104 of 159 M.Sc. Botany with specialization in Plant Biotechnology Programme Articulation Matrix – Weights
Sem Course Course title PO1 PO2 PO3 PO4 PO5 PO6 PO7 code HLBP11 Plant Diversity 39 12 4 4 3 - - HLBP12 Inheritance Biology 25 21 33 9 - - - Taxonomy of I HLBP13 39 15 3 14 1 - - Angiosperms HLBP1L Practical I 33 31 10 7 - - - HLBP1E Elective I 33 6 7 8 - - - Plant Anatomy and 33 18 9 6 - - - HLBP21 Embryology HLBP22 Biochemistry 15 21 9 6 2 - - II HLBP23 Microbiology 31 24 5 1 - - 1 HLBP2L Practical II 18 30 9 6 2 - - HL2E Elective II 25 16 12 9 - - - Cell and Molecular 27 19 7 4 - 1 - HLBP31 Biology Environmental 39 18 33 6 - - - HLBP32 III Microbiology HLBP33 Plant Physiology 33 33 12 3 2 - - HLBP3L Practical III 27 33 25 2 3 - - HLBP3E Elective III 31 24 15 6 - - - HLBP41 Plant Ecology 33 8 4 5 - 1 - Applied 20 12 21 4 9 - - HLBP42 Biotechnology IV HLBP4L Practical IV 20 12 21 4 9 - - HLBP4E Elective IV 27 33 22 9 2 - - Project and viva 45 18 27 24 1 3 3 HLBP4P voce Total Weightage of all courses contributing to POs 593 404 288 137 34 5 4
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Page 105 of 159 M.Sc. Botany with specialization in Plant Biotechnology Programme Articulation Matrix - Weighted percentage
Sem Course Course title PO1 PO2 PO3 PO4 PO5 PO6 PO7 code HLBP11 Plant Diversity 6.58 2.97 1.39 2.92 8.82 0 0 Inheritance HLBP12 Biology 4.22 5.2 11.46 6.57 0 0 0 I Taxonomy of HLBP13 Angiosperms 6.58 3.71 1.04 10.22 2.94 0 0 HLBP1L Practical I 5.56 7.67 3.47 5.11 0 0 0 HLBP1E Elective I 5.56 1.49 2.43 5.84 0 0 0 Plant Anatomy HLBP21 and Embryology 5.56 4.46 3.13 4.38 0 0 0 HLBP22 Biochemistry 2.53 5.2 3.13 4.38 5.88 0 0 II HLBP23 Microbiology 5.23 5.94 1.74 0.73 0 0 25 HLBP2L Practical II 3.04 7.43 3.13 4.38 5.88 0 0 HL2E Elective II 4.22 3.96 4.17 6.57 0 0 0 Cell and HLBP31 Molecular Biology 4.55 4.7 2.43 2.92 0 20 0 Environmental III HLBP32 Microbiology 6.58 4.46 11.46 4.38 0 0 0 HLBP33 Plant Physiology 5.56 8.17 4.17 2.19 5.88 0 0 HLBP3L Practical III 4.55 8.17 8.68 1.46 8.82 0 0 HLBP3E Elective III 5.23 5.94 5.21 4.38 0 0 0 HLBP41 Plant Ecology 5.56 1.98 1.39 3.65 0 20 0 Applied HLBP42 Biotechnology 3.37 2.97 7.29 2.92 26.47 0 0 IV HLBP4L Practical IV 3.37 2.97 7.29 2.92 26.47 0 0 HLBP4E Elective IV 4.55 8.17 7.64 6.57 5.88 0 0 Project and viva HLBP4P voce 7.59 4.46 9.38 17.52 2.94 60 75 Total Weighted percentage of all courses contributing to POs 99.99 100.02 100.03 100.01 99.98 100 100
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Page 106 of 159 THE STANDARD FIREWORKS RAJARARATNAM COLLEGE FOR WOMEN (Autonomous), SIVAKASI DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER I CORE COURSE HLBP11 – PLANT DIVERSITY (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course outcomes (CO): On successful completion of the course, the learners should be able to CO1: recall the occurrence and structure of cryptogams and Gymnosperms. CO2: analyse the classification and lifecycle pattern of different groups of plants. CO3: explain the economic importance of different groups of plants. CO4: relate the evolution of sporophyte in Pteridophytes. CO5: identify the existence of life on earth in various eras.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 ------CO2 9 3 - - - - - CO3 9 3 - 3 - - - CO4 9 3 1 1 - - - CO5 3 3 3 - 3 - - Weightage of 39 12 4 4 3 - - the course Weighted 6.58 2.97 1.39 2.92 8.82 0 0 percentage of Course contribution to POs
Unit-I (18 Hours) Classification of Algae by Fritsch,1935. Thallus construction, different life cycle patterns. Distribution, structure and life cycle of the following - Cyanophyta - Anabaena, Chlorophyta – Oedogonium, Phaeophyta – Ectocarpus, Rhodophyta - Gracillaria. Economic importance of Algae. Classification of Fungi by Alexopoulos and Mims, 1979. Distribution, structure and life cycle of the following - Ascomycetes - Aspergillus and Basidiomycetes - Agaricus. Economic importance of Fungi. Unit-II (18 Hours) Classification of Bryophytes by Rothmaler, 1951. Distribution, structure and life cycle of the following - Anthocerotopsida - Anthoceros, Bryopsida – Polytrichum. Fossil Bryophytes. Evolution of sporophytes in Bryophytes.
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Page 107 of 159 Unit-III (18 Hours) Classification of Pteridophytes by Sporne, 1966. Characteristic features and life cycle pattern – apogamy and apospory. Evolution of sporophyte – telome theory, stelar variation, Enation theory of Bower. Distribution, structure and life cycle of the following- Lycopsida – Selaginella, Filicopsida – Gleichenia.
Unit-IV (18 Hours) Classification of Gymnosperms by Chamberlain, 1933. Internal structure, reproduction and Life cycle of the following - Coniferales – Cupressus, Taxales – Araucaria. Economic importance of Gymnosperms.
Unit – V (18 Hours) Contribution by Birbal Shahini. Geological time scale, fossilization types of fossils, techniques used for fossil study. Fossil Pteridophytes- Calamitales - Calamites. Fossil Gymnosperms- Ginkgoales – Ginkgo.
Reference Books: 1. Singh,V., Pandey P.C., and Jain D.K. (1998) A Text Book of Botany, Rastogi Puplications, II Revised Edition. Unit I - Page No : 19-21, 68-72, 93-100,142-147,153-160 and 187-191. Unit II - Page No : 60-68, 97-104,111-114. Unit III – Page No : 11, 66 – 79, 135 – 139, 183-186, 187-191. Unit IV – Page No : 6. Unit V – Page No : 14-22, 80-83, 192-193. 2. Pandey, B.P., (2003), College Botany, Vol.I, S. Chand and Co., New Delhi, IIIEdition. Unit I - Page No: 25 - 30, 129 -134,224 - 230,249 – 255. Unit II - Page No: 11 – 17, 110 – 133, 171 – 190. 3. Pandey, B.P., (2013), College Botany, VoII. S. Chand and Co., New Delhi, I Edition. Unit- III -Page No: 47 – 69, 130 - 138. Unit-V - Page No: 46-59. 4. Kumaresan ,V., and Annie Ragland., 2010.Pteridophytes, Gymnosperms and Palaeobotany, Saras Publication , Nagercoil, Unit III - Page No: 113 - 131. Unit IV - Page No: 268 - 291. 5. Vashishta, B.R., 2013.Botany for degree students, Algae, S. Chand and Co., New Delhi, Revised Edition.
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Page 108 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER I CORE COURSE HLBP12-INHERITANCE BIOLOGY (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 04
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: relate the knowledge on laws of inheritance, genetic basis of loci and alleles. CO2: compute the various steps in protein synthesis and protein modification. CO3: analyze the chromosome structure and genome organization. CO4: discuss the effect of linkage and crossing over. CO5: interpret on non-allelic genic interactions and population genetics.
CO-PO Mapping table (Course Articulation Matrix) POs PO1 PO2 PO3 PO4 PO5 PO6 PO7
COs CO1 9 3 3 - - - - CO2 1 3 9 - - - - CO3 9 3 3 - - - - CO4 3 3 9 - - - - CO5 3 9 9 9 - - - Weightage of 25 21 33 9 - - - the course Weighted 4.22 5.2 11.46 6.57 0 0 0 percentage of Course contribution to POs
Unit – I (18 Hours) Molecular and chemical organization of chromosomes –Euchromatin and heterochromatin, karyotype, banding patterns. Special types of chromosomes – polytene, lampbrush, B-chromosomes, Chromatin organization – chromosome structure and packaging of DNA. Nuclear DNA content, C-value paradox, cot curve and its significance.
Unit - II (18 Hours) Mendelian theory of inheritance - trihybrid ratio and test cross ratios. Interaction of non-allelic genes - complementary, dominant epistatic, recessive epistatic and duplicate ratios.Multiple alleles - Blood group in human beings. Eye colour in
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Page 109 of 159 Drosophila. Polygenic inheritance - skin colour in human beings, corolla length in Nicotiana.
Unit - III (18 Hours) Linkage –theories of linkage - complete, incomplete linkage. Crossing over – molecular mechanism and theories of crossing over. Cytological basis of crossing over – Creighton and McClintock’s in corn. Homologous recombination of genes – holiday junction. Construction of genetic map – two point cross – three point test cross – maize. Pedigree analysis – lod score method. Tetrad analysis –ordered tetrads.
Unit - IV (18 Hours) Fine structure of gene - cistron, muton, recon, exons and introns. Genetic code - properties and wobble hypothesis. Mechanism and regulation of protein synthesis. Post translational modification of proteins. RNA interference - siRNA.
Unit – V (18 Hours)
Mutation - molecular basis of mutation, physical, chemical mutagens and their mode of action. Detection of mutation in higher plants. Chromosomal aberrations and polyploidy.
Reference Books: 1. Gupta P.K. (2011) Genetics, Rastogi Publications, Meerut. Unit- I- Page No: 58-68, 321-323 Unit- II- Page No: 19 -51 Unit-III- Page No: 92-115 Unit-IV - Page No: 152-153and357-391. Unit-V - Page No: 210-243. 2. Verma, P.S. and Agarwal V.K. (Reprint 2012) Genetics, S.Chand &company Ltd, NewDelhi. 3. Parihar, P, (2005) A text book of Basic and Molecular Genetics, Publishing Student Edition, Jodhpur, II Edition. 4. Cooper, G.M. and Hausman, R.E. (2007), The Cell- A Molecular Approach, ASM Press, Washington, D.C., IV Edition. 5. David Friefelder, (2005) Molecular Biology, Narosa Publishing House, New Delhi.II Edition.
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Page 110 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER I CORE COURSE HLBP13 - TAXONOMY OF ANGIOSPERMS (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 04
Course outcomes (CO): On successful completion of the course, the learners should be able to CO1: analyze different systems of classification and its historical account with its merits and demerits. CO2: summarize the rules and recommendations of ICN, role of BSI and herbarium. CO3: explain the use of digital resources in plant taxonomy. CO4: demonstrate the key characters and economic importance of angiospermic families. CO5: compare the vegetative and floral characters within and between angiospermic families.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 - - 3 - - - CO2 9 3 - - - - - CO3 3 - 3 1 - - - CO4 9 3 - 1 - - - CO5 9 9 - 9 1 - - Weightage of 39 15 3 14 1 - - the course Weighted percentage of Course 6.58 3.71 1.04 10.22 2.94 0 0 contribution to POs
Unit – I (18 Hours) A brief historical account of the classification of Angiosperms. Systems of classification: Linnaeus, Bentham and Hooker’s, Carls Bessey, Engler and Prantl, Hutchinson and APG III system (in brief) – merits and demerits.
Unit – II (18 Hours) International Code of Botanical Nomenclature - typification, principles of priority, effective and valid publication, author citation, retention and rejection of names. Chemotaxonomy, numerical taxonomy and cladistics.
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Page 111 of 159 Unit – III (18 Hours) Collection of plants and preparation of herbarium, achievements of BSI, databases and softwares for plant taxonomy – e-floras, digital atlas and electronic herbaria. Key of plant taxonomy – conventional – indented and bracketed keys. Computerised taxonomic keys – delta and polyclave. Role of DNA in plant taxonomy - DNA bar coding. Floras, Monographs and revisions.
Unit – IV (18 Hours) Salient features and floral characters of the following families: Magnoliaceae, Nymphaeceae, Capparidaceae, Tiliaceae, Anacardiaceae, Rosaceae, Moringaceae, Combretaceae, Myrtaceae, and Apiaceae.
Unit – V (18 Hours) Salient features and floral characters of the following families: Sapotaceae, Oleaceae, Convolvulaceae, Bignoniaceae, Verbenaceae, Nyctaginaceae, Orchidaceae, Commelinaceae, Arecaceae, and Cyperaceae.
Reference Books: 1. Sharma, O.P., (2009), Plant Taxonomy, Tata McGraw Hill, New Delhi, II Edition. Unit I - Page No: 8-12, 21-22, 24-33. Unit II –Page No: 115-126. Unit III - Page No: 152 – 157. Unit IV & V–Page No: 214-525 (Refer pages for selected families). 2. Lawerance, H.M, (1967), Taxonomy of vascular plants, Oxford and IBH publishing company, New Delhi, I Edition. Unit III – Page No: 263 – 283. 3. Rendle, A.B, (1979), Classification of flowering plants, Vol. I and II. Cambridge University press, London,V Edition. Unit II - Page No: 76 – 87. Unit III - www.plantatlas.eu https://en.wikipedia.org/wiki/virtualherbarium delta-intkey.com crescentbloom.com/ii/l/18.htm
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Page 112 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY CORE COURSE SEMESTER I HLBP1L-PRACTICAL I (Plant Diversity, Inheritance Biology and Taxonomy of Angiosperms) (For those admitted in June 2017 and later)
Contact hours per week : 2+2+2 Total number of hours per semester : 30+30+30 Number of credits 04
Course outcomes (CO): On successful completion of the course, the learners should be able to CO1: illustrate the preparation of whole mount and sectioning of plant materials. CO2: analyze genetic problems. CO3: determine the role of several genes in inheritance. CO4: identify the family of an angiospermic plant with its taxonomical description. CO5: construct taxonomic keys and make use of herbarium.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 3 1 - - - - CO2 9 1 - - - - - CO3 3 9 3 3 - - - CO4 9 9 3 1 - - - CO5 9 9 3 3 - - - Weightage of 33 31 10 7 - - - the course Weighted percentage of Course 5.56 7.67 3.47 5.11 0 0 0 contribution to POs
Plant Diversity 1. Make suitable whole mount preparation of the following types Anabaena, Oedogonium, Ectocarpus, Aspergillus 2. Make suitable slide preparation of the following Agaricus – T.S. of gills Anthoceros, Polytrichum – T.S. of thallus, Selaginella–T.S of stem Auracaria, Cupressus, and Ginkgo– T.S. of stem 3. Spot at sight Oedogonium – hold fast, macrandrous male, Oogonia Ectocarpus – unilocular, plurilocular
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Page 113 of 159 Aspergillus - Sporangia Agaricus –T.S of gills Polytrichum – Protonema, Antheridia V.S and L. S of sporophyte Anthoceros – T.S of thallus, antheridia V.S, L.S of sporophyte Selaginella – T.S of rhizophore, T.S of root, T.S of stem, L.S of cone Gleichenia – T.S of petiole. Calamites – habit, T.S. of root, T.S. of stem and L.S of cone. Inheritance Biology To study polygenic inheritance of Quantitative traits in plants - height of fruits chillies and Polyalthia Solve the genetic problems 1. Trihybrid ratio and test cross. 2. Interaction of genes. 3. Multiple alleles – Blood group. 4. Eye colour in Drosophila. 5. Sex linked inheritance. 6. Linkage. 7. Crossing over. 8. Construction of genetic map. 9. Two point test cross. 10. Three point test cross. 11. Pedigree analysis. 12. Tetrad analysis. Spot at sight 1. Special types of chromosomes – polytene, lampbrush, B chromosomes. 2. Chromatin organization. 3. C-value paradox. 4. Cot curve. 5. Holiday junction. 6. Si RNA. 7. Mutagens. 8. Chromosomal aberration. 9. Polyploidy Taxonomy of Angiosperms 1. Prepare a yoked key for the plants supplied. 2. Prepare a bracketed key for the plants given. 3. Describe the plant in technical terms. Draw labeled diagrams of the floral parts including the longitudinal section of the flower and construct the floral diagram. 1. Magnoliaceae 2. Nymphaeceae 3. Capparidaceae 4. Tiliaceae 5. Anacardiaceae 6. Rosaceae 7. Moringaceae 8. Combertaceae 9. Myrtaceae
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Page 114 of 159 10. Apiaceae 11. Sapotaceae 12. Oleaceae 13. Convolvulaceae 14. Bignoninaceae 15. Verbenaceae 16. Nyctaginaceae 17. Orchidaceae 18. Commelinaceae
19. Arecaceae 20. Cyperaceae Study tour Submission of 15 Herbarium sheets correctly identified for external valuation. Submission of record and field note book.
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Page 115 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER I ELECTIVE COURSE HLBP1E1 - BIOINSTRUMENTATION (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course outcomes (CO): On successful completion of the course, the learners should be able to CO1: recall the principles and applications of microscopy. CO2 :demonstrate the preparation of gel and detection of nucleic acid by electrophoresis. CO3: classify the various types of chromatographic techniques and its applications. CO4: analyse the working principle and applications of colorimetry, spectrophotometer, flame photometer and NMR. CO5: explain the stages involved in the preparation of permanent slides.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 - - 3 - - - CO2 9 - 3 3 - - - CO3 9 3 1 1 - - - CO4 9 3 - - - - - CO5 3 - 3 1 - - - Weightage of 33 6 7 8 - - - the course Weighted percentage of 5.56 1.49 2.43 5.84 0 0 0 Course contribution to POs
Unit – I (18 Hours) Microscopy - basic principle, applications, types of microscopes - phase contrast, dark field, and flurorescent microscopes. Electron microscopes - types - SEM, TEM and AFM - principle, working mechanism and applications. Sample preparation for EM.
Unit – II (18 Hours) Electrophoresis- principle, types and methods (PAGE, AGE, SDS -PAGE) - preparation of gel, composition, sample preparation, running and detection. Factors affecting electrophoresis.
Unit – III (18 Hours)
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Page 116 of 159 Chromatography techniques - principle - paper, column, ion exchange chromatography, TLC, HPLC, GC-MS and its applications.
Unit – IV (18 Hours) Colorimetry - principle, Beer-Lambert law and its application. Single and double beam spectrophotometers, principle and biological applications of UV-visible spectrophotometers. Atomic absorbtion of spectrophotometry - principle and application. Flame photometer and NMR - principle and applications.
Unit – V (18 Hours) Microtechnique - working principle of microtome - fixation, infilteration, embedding, sectioning, staining, double staining, dehydration, cleaning and mounting. Microslide preparation.
Reference Books: 1. Gurumani,N., (2006), Research Methodology for biological sciences, MJP Publication, Chennai, I Edition. Unit I - Page No: 179-248 Unit II - Page No: 319-372 Unit III - Page No: 277-309 Unit IV - Page No: 375-402 2. KeithWilson and JohnWalker., (2004), Practical biochemistry, principles and techniques,Cambridge University press, V Edition. Unit II - Page No: 580-612 Unit III - Page No: 619-681 Unit IV - Page No: 453-485 3. Marimuthu R., (2008), Microscopy and Microtechnique, MJP publishers, Chennai. Unit I - Page No: 1-5, 113-116, 137-151 & 191-198. Unit V - Page No : 251-345 4. Veerakumari L., (2006), Bioinstrumentation, MJP Publishers, Chennai.
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Page 117 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER I ELECTIVE COURSE HLBP1E2-HERBAL MEDICINE (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: illustrate the basic principles and history of medicinal practices. CO2: discuss the ways to cultivate herbs by different methods. CO3: conclude the collection, processing and storage of herbal drugs. CO4: justify the importance of major herbs in day today life. CO5: identify the adulteration of drug and its evaluation through various testing methods.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 - - 3 - - - CO2 9 - 3 3 - - - CO3 9 3 1 1 - - - CO4 9 3 - - - - - CO5 3 - 3 1 - - - Weightage of 33 6 7 8 - - - the course Weighted percentage of 5.56 1.49 2.43 5.84 0 0 0 Course contribution to POs
Unit - I (18 Hours) Basic principles and history of various medical practices in India (Siddha, Ayurveda, Unani). Scope of raw drugs of plant origin. Importance and relevance of Herbal drugs in Indian system of medicine. Cultivation – Sexual and asexual methods - factors affecting cultivation. Collection and processing of herbal drugs and storage
Unit - II (18 Hours) Drug adulteration – methods and types. Deterioration of drugs – primary factors. Mould, bacteria, insect pest. Control of insect pest. Drug evaluation – morphological, microscopic, chemical, physical and biological evaluation.
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Page 118 of 159 Unit – III (18 Hours) Aromatic and cosmetic herbs with special reference to binomial, family and morphology of the useful parts, active principle and uses of the following - Acorus calamus, Elettaria cardamomum, Piper longum, Mentha arvensis, Curcuma longa, Hibiscus – rosa – sinensis, Aloe vera. Lawsonia inermis, Carica papaya, Santalum album, Papaver somniferum and Pimpinella anisum.
Unit – IV (18 Hours) Study of the phytochemicals and therapeutic value of the following -alkaloids, glycosides, saponins and tannins. Study of crude drugs from Atropa belladonna (atropine), Digitalis purprea (digitalin), Cassia angustifolia (sennosides A and B), Solanum nigrum. (solasodine) and Terminalia chebula (chebulic acid).
Unit – V (18 Hours) Study of the following plants with special reference to binomial, family and morphology of the useful parts, active principle and uses. Roots – Rauwolfia serpentina, Gentiana lutea, Withania somnifera. Rhizome – Gingiber officinale, Helleborus niger, Ferula asafoetida. Bark – Acacia catechu, Azadirachta indica, Rhamnus purshiana. Leaves – Eucalyptus globulus, Adhatoda vasica, Andrographis paniculata. Flowers – Catharanthus roseus, Ervatamia coronaria, Eugenia caryophyllata. Fruits – Vanilla planifolia, Momordica charantia, Punica granatum. Seeds - Theobroma cocoa, Brassica nigra, Colchicum autumnale.
Reference Books: 1. Chopra, R.N., Nayar S.L. and I.C.Chopra, (1956), Glossary of Indian Medicinal Plants, CSIR, New Delhi, I Edition. 2. Gamble, J.S., (1979), The Flora of the Presidency of Madras, Volume I, II and III, Bishen Singh Mahendra Pal Singh, Dehradun, II Edition. 3. Kokate, C.K., Purohit, A.P. and Gokhale, (2007), Pharmacognosy, Nirali Prakasam, Pune, XXXVII Edition., 4. Mathew, K.M., (1983), The Flora of the Tamil Nadu Carnatic, The Rapinat Herbarium, Trichy, I Edition.
Study material will be provided.
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Page 119 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY CORE COURSE SEMESTER II HLBP21–PLANT ANATOMY AND EMBRYOLOGY (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learner should be able to CO1: summarize about meristematic, epidermal and ground tissue systems. CO2: explain the origin, types, structure and functions of vascular tissue system. CO3: elaborate sporogenesis on the basis of their structure and development. CO4: analyze the process of fertilization and sexual incompatibility. CO5:discuss endosperm, polyembryony, embryo development, apomixis and parthenocarpy in plants.
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 ------CO2 3 3 - - - - - CO3 9 3 3 - - - - CO4 9 3 3 3 - - - CO5 3 9 3 3 - - - Weightage of 33 18 9 6 - - - the course Weighted percentage of 5.56 4.46 3.13 4.38 0 0 0 Course contribution to POs
Unit - I (18 Hours) Plant morphogenesis, polarity, symmetry. Meristems - classification -based on stage of development - based on origin of initiating cells - position in plant body. Functions and theories - The Histogen theory, Tunica - corpus theory. Epidermal tissue system and functions - epidermis, stomata, trichomes. Ground tissue system and functions - cortex, endodermis, pericycle and pith.
Unit – II (18 Hours) Vascular tissue system - xylem, phloem and cambium. origin, types and structure. Secondary xylem - ontogeny, structure and function - non porous and porous wood, growth layers, sap wood and heart wood. Arrangement of vessels in secondary xylem of dicots. Phloem and its constituents, transfer cells. Various types of anomalous secondary
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Page 120 of 159 thickening - Nyctanthes, Dracaena, Achyranthes and Boerhaavia. Bark anatomy of Bombax and Tectona. Rhytidome. Nodal anatomy.
Unit – III (18 Hours) Microsporogenesis - anther wall layers, sporogenous tissue, development of microsporangium. Development of male gametophyte - formation of generative and vegetative cell. Structure and development of pollen wall, NPC system of apertures, pollen wall features. Megasporogenesis - types of embryo sac based on Maheswari and BGL Swamy’s classification. Structure of mature embryo sac. Haustorial behavior and nutrition of embryo sac.
Unit - IV (18 Hours) Fertilization – pollen germination, factors influencing pollen (in vivo and in vitro) germination, pollen tube wall and pollen tube growth. Entry of pollen tube into the ovule, pollen tube discharge, passage of sperms, gametic fusion, double fertilization and its significance. Sexual incompatibility - genetic and biochemical basis of incompatibility, methods to overcome incompatibility. Endosperm - free nuclear, cellular, helobial and ruminate endosperm. Haustorial behavior and functions of endosperm.
Unit – V (18 Hours) Polyembryony - classification, causes, types - Cleavage Polyembryony and embryos from cells of embryo sac other than egg, more than one embryo sac in the same ovule, activation of some sporophytic cells of the ovule. Experimental induction of polyembryony and practical value of polyembryony. Embryo development and classification (BGL Swamy) - development of dicot - Chenopodial type eg. Chenopodium bonus-henricus and monocot embryo - eg. Najus. Apomixis - causes, vegetative reproduction, agamospermy - adventive embryony, diplospory - apospory. Significance of apomixis. Parthenocarpy.
Reference Books: 1. Pandey, B.P, (reprint 1984), Plant Anatomy, S. Chand & company Ltd, New Delhi. Unit I – Page No. 89-120, 121-130, 134-155 Unit II – Page No. 155-160, 163-169, 239-251, 253-255, 269-272, 273-274, 286-289, 291-294 2. Bhojwani, S.S and S.P. Bhatnagar, (1985), Embryology of Angiosperms, Vikas Publishing House Pvt.Ltd, New Delhi, II Edition. Unit- III - Page No: 13-37, 55-59, 82-89, 90-105. Unit- IV – Page No: 124-148, 149-175, 177-198. Unit- V – Page No: 235-244, 249-251, 253-263. 3. Cutter, E.G., (1971), Plant Anatomy, Vol. I and II, Edward Arnold Publishing Ltd. London, II Edition. 4. Esau, K, (1965), Plant Anatomy, Wiley Eastern Ltd, New Delhi, II Edition. 5. Fahn, A., (1974), Plant Anatomy, Pergamon Press, Oxford, II Edition. 6. Maheswari, P., (1950), Introduction to embryology of Angiosperms, Mc Graw Hill. New Delhi, I Edition.
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Page 121 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER II CORE COURSE HLBP22 - BIOCHEMISTRY (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 04 Course Outcomes (CO): On successful completion of the course, the learner should be able to CO1: discuss the classification, properties and metabolism of carbohydrates. CO2: elaborate the classification, structure, biosynthesis and properties of amino acids and lipids. CO3: explain the classification, structure and properties of proteins and vitamins. CO4: illustrate the classification, principle and mechanism of enzyme action. CO5: analyze the structure, types, composition and biosynthesis of nucleic acids.
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 - 9 - - - - - CO2 3 9 - - - - - CO3 - 3 3 - - - - CO4 9 - 3 3 1 - - CO5 3 - 3 3 1 - - Weightage of 15 21 9 6 2 - - the course Weighted percentage of 2.53 5.2 3.13 4.38 5.88 0 0 Course contribution to POs
Unit I (18 Hours) Carbohydrates - classification and general properties of mono, disaccharides - maltose, sucrose, lactose. Polysaccharides - homo polysaccharides - starch and inulin. Heteropolysaccharides - peptidoglycan, glycoprotein. Metabolism - Glycogenesis, glycolysis, HMP pathway, citric acid cycle - electron transport chain, oxidative phosphorylation, Gluconeogenesis.
Unit II (18 Hours) Amino acids - structure and properties. Classification based on precursors composition of side chain, number of amino and carboxylic groups, polarity of the side chain and biosynthetic precursors. Biosynthesis of aromatic amino acids and metabolism - Oxidative deamination and urea cycle.
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Page 122 of 159 Proteins - classification, properties and structure - Primary, Secondary, Tertiary and Quaternary structures. Ramachandran plot.
Unit III (18 Hours) Lipids - classification, structure and properties. Simple lipids - fats, oils and waxes. Compound lipids - phospholipids, glycolipids. Derived lipids - steroid, monoterpenes, carotenoids. Fatty acids - saturated and unsaturated. Biosynthesis of triacyl glycerol and phospholipid and degradation. ß oxidation of fatty acids. Vitamins - classification - sources, structure and deficiency diseases of fat soluble and water soluble vitamins.
Unit IV (18 Hours) Enzymes - classification, principles of catalysis, enzyme kinetics, Enzyme regulation - allosteric enzymes, covalent modifications, mechanism of enzyme catalysis, isozymes. Mechanism of enzyme action - Michalies - Menton equation, Line weaver - Burk equation, Fischer’s lock and key model, Koshland’s induced fit model. Enzyme inhibition - competitive, non competitive and feedback inhibition.
Unit V (18 Hours) Nucleic acids - chemical composition. Structure of DNA, Types – double stranded DNA, single stranded DNA, A-DNA, B-DNA and Z-DNA. Biosynthesis of purine and pyrimidine. DNA replication - Unit of replication, enzymes involved in replication origin and replication fork, fidelity of replication, extra chromosomal replicons, DNA damage, repair mechanisms and recombinations.
Reference Books: 1. Jain, J.L., (2014), Elementary Biochemistry, S.Chand and Co., Ltd., New Delhi. Unit I - Page No: 91-94,116-138,143-149 Unit II - Page No: 166-178,800-809,251-275,190-217. Unit III - Page No: 280-335, 1303-1374. Unit IV - 103-107, 110-115. Unit V - Page No: 341-354,361-376,377-378,851-856. 2. Gupta, P.K. (2011), Genetics, Rastogi publications. Unit V - 292- 319, 3. Gardner, (2006), Principles of Genetics, John Willey & Sons.INC. Unit IV - 110-122. 4. Michael Cox., David. L. Nelson, Lehninger, (reprint 2004), Principles of Biochemistry, Kalyani Publishers, New Delhi.
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Page 123 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER II CORE COURSE HLBP23 - MICROBIOLOGY (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 04
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: identify the characters and methods of classification of microbes. CO2: explain the structure and multiplication of bacteria and viruses. CO3: analyze the impact of microflora in soil, air and water. CO4: elaborate the symptoms, transmission, diagnosis and control of microbial diseases caused to human beings. CO5: assess the cellular and molecular basis of immune responsiveness and the ways to detect it.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 1 ------CO2 9 3 1 - - - - CO3 9 9 3 - - - - CO4 3 3 - 1 - - 1 CO5 9 9 1 - - - - Weightage of 31 24 5 1 - - 1 the course Weighted percentage of 5.23 5.94 1.74 0.73 0 0 25 Course contribution to POs
Unit – I (18 Hours) Bacteria - General characteristics, Classification- Bergey’s manual. Ultra structure of bacterial cell. Staining methods - Gram and acid fast staining, endospore staining. Sterilization - Physical and chemical - principle, agents, mode of action and application. Culture of microorganism - continuous, synchronous and batch culture, growth phases, factors affecting bacterial growth.
Unit II (18 Hours) Virus - classification based on genetic material. Morphology of viruses - size, shape, structure, envelope, nucleic acids and proteins. TMV- structure, genome
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Page 124 of 159 multiplication, transmission and symptoms. HIV - structure, genome organization, transmission. Replication and assembly. T4 Bacteriophage - morphology and multiplication. Lytic and lysogenic life cycle.
Unit – III (18 Hours) Soil Microbiology - microorganisms, interactions among soil microorganisms - beneficial and harmful effects. Air microbiology - significance and control of air borne microorganisms. Microbiology of water - marine and freshwater microbiology, microbial analysis of water purity - sanitary test of coliforms, most probable number of coliforms. Purification of water - sedimentation, filtration and disinfection. Sewage treatment - primary, secondary and tertiary treatment.
Unit – IV (18 Hours) Disease caused by microbe - symptoms etiology, transmission, diagnosis and control with special reference to Bacterial diseases - Diphtheria, Tuberculosis. Fungal diseases - Candidiasis, Mycetoma. Protozoan diseases - Amoebiosis, Malaria. Viral diseases - Small pox, Hepatitis.
Unit – V (18 Hours) Immunology- definition - defense mechanism - innate and acquired resistance. Phagocytosis. Immune system - types - humoral and cell mediated immune system. Antigen - antibody reaction. Primary and secondary lymphoid organs. Immunoglobulin structure and their types, immunoassay - RIA, ELISA and hybridoma technology.
Reference Books: 1. Pelczar, JR., Chan, E.C.S., Krieg,R. (reprint 1998), Microbiology, Tata Mc Graw – Hill Publishing Company Limited, New Delhi. Unit I – Page No: 37-49. 2. Dubey, R.C. and D.K.Maheswari, (reprint 2012), Microbiology, S.Chand & Company LTD, New Delhi. Unit I - 50-71, Unit- II - Page No: 305-317, 330-333, 352-363 Unit- V - Page No: 428- 458 3. Sharma, P.D., (reprint 2005), Environmental Microbiology, Narosa publishing house, New Delhi. Unit- III - Page No: 53-57 and 43-49. 4. Rajan, S., and Selvi Christy. R., (2015), Essential of Microbiology, Anjanaa book house, Chennai. Unit I – 95-97. Unit III - 251-260, 270-273, 531-570. Unit IV - 538-539,541-542, 553-564, 558-561, 5. Prescott, Harley and Klein’s, (2008), Microbiology, Mc Graw - Hill publishing company.
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Page 125 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER II CORE COURSE HLBP2L-PRACTICAL II (Plant Anatomy and Embryology, Biochemistry, Microbiology) (For those admitted in June 2017 and later)
Contact hours per week : 2+2+2 Total number of hours per semester : 30+30+30 Number of credits 04
Course Outcomes (CO): On successful completion of the course, the learner should be able to CO1: make use of maceration technique to isolate and identify cells. CO2: identify the anomalous secondary growth and bark anatomy of plants. CO3: examine pollen germination, embryo development and endosperm . CO4: estimate the biomolecules qualitatively and quantitatively. CO5: experiment the preparation of media, staining, Isolation and identification of microbes.
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 9 - - - - - CO2 - 9 - - - - - CO3 3 3 3 - - - - CO4 9 - 3 3 1 - - CO5 3 9 3 3 1 - - Weightage of 18 30 9 6 2 - - the course Weighted percentage of 3.04 7.43 3.13 4.38 5.88 0 0 Course contribution to POs Plant Anatomy Experiments Maceration technique – isolation and measurements of cells – tracheids, vessels and fibres. Slide preparation Anomalous secondary growth – Nyctanthes, Dracaena, Achyranthes and Boerhaavia. Bark anatomy of Bombax and Tectona. Spot at sight Meristem studies – Shoot apex. Nodal Anatomy – unilacunar, trilacunar, multilacunar.
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Page 126 of 159 Embryology Experiments Effects of sucrose / pH on Pollen germination Dissection of any two stages of embryo. Dissection of haustorial endosperm of cucumber. Spot at sight T.S of mature anther Integument- Aril – Pithecolobium dulce, Myristica fragrans Ovule types Polyembryony – Cleavage Polyembryony – Citrus medica Parthenocarpy – Seedless grapes Biochemistry Experiments Qualitative test for carbohydrates, proteins and fats. Estimation of sugar – Anthrone method. Isoelectric point of amino acid. Separation of amino acids by paper chromatography. Estimation of protein – Lowry’s method. Saponification value of fat and oil. pKa value of acetic acid. Estimation of peroxidase, amylase, catalase and MDA Spot at sight DNA replication Replication fork Extra chromosomal replicons DNA damage Microbiology Experiment Preparation of culture media- • Nutrient agar • Nutrient broth Staining • Simple • Gram stain • Acid fast • Endospore staining • Negative staining Isolation and identification of microbes of air, water and soil. • Serial dilution technique • Streak plate method • Pour plate method • Spread plate technique
Spot at sight Ultra structure of E.coli Virus • TMV • T4 Bacteriophage • Lytic and lysogenic cycle
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Page 127 of 159 • HIV Antigen antibody reaction. RIA ELISA Hybridoma technology
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Page 128 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY SEMESTER II ELECTIVE COURSE HLBP2E - FOOD PRESERVATION (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learner should be able to CO1: outline the basic methods and techniques of food preservation. CO2: illustrate the protocol for fruit juices and beverages. CO3: make use of various preparation and preservation techniques. CO4: assess the use of food additives and sweetners. CO5: analyze food adulteration and various forms of food packaging materials.
POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 1 - 3 - - - - CO2 3 1 - - - - - CO3 9 3 3 - - - - CO4 3 9 3 - - - - CO5 9 3 3 9 - - - Weightage of 25 16 12 9 - - - the course
Unit - I (18 Hours) Introduction - classification of food - perishable foods, semi -perishable foods and non - perishable foods. Principles of food preservation - prevention - delay of microorganisms, delay of self - decomposition of the food, damage from insects, animals and mechanical causes. Methods of food preservation - bacteriostatic methods – asepsis, removal of microorganisms, maintenance of anaerobic conditions, uses of high temperature, freezing, drying and dehydration and canning.
Unit – II (18 Hours) Fruit juice beverages - classification - general methods of fruit juice beverages preparation - Pine apple, Citrus and Mango juice - selection and preparation of fruits, extraction of juice, preservation of juice, clarification of juice, filling, pasteurization of juice, cooling and storage. Preparation of Orange squash, Rose syrup, Nannari and Grape crush - selection and preparation of fruits, extraction of juice, preservation of juice, clarification of juice, filling, pasteurization of juice, cooling and storage. Unit – III (18 hours) Dried / dehydrated products - carrot and Tomato - cleaning, washing, scraping, cutting, blanching, cooling, packing and storage.
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Page 129 of 159 Preparation of jam - mixed fruit jam - selection of fruits, washing, peeling, cooling and storage. Mushroom dishes - recipes - washing, peeling, cooking. Pickles - garlic and mixed vegetable pickles.
Unit – IV (18 hours) Food additives - product consistency, nutritional value, palatability, flavor and leavening. Natural and Artificial sweeteners - Aspartame and Saccharin - composition and digestion, effect of heating on aspartame, consumer safety, aspartame and regulatory bodies and ADI. Coloring agents, Flavouring agents and Preservatives (SB, KMS, citric acid and vinegar).
Unit –V (18 hours) Food adulteration - quality standards - Food standards in India - prevention of food adulteration Act 1954, FPO, ISI, BIS, AGMARK, Vegetable oil control order (1976), and the meat product order. Food packaging materials and forms - meats, tin can, glass containers, flexible packaging material, hazards of plastic packaging, laminates, retortable pouches.
Reference Books: 1. Sudesh Jood and Neelam Khetarpaul, (2002), Food preservation, Agrotech Publishing Academy, Udaipur, I Edition. Unit I – Page. No: 1-2 and 6- 34. Unit II – Page. No: 84-95. Unit III – Page. No: 82-83, 86, 95-102 and 111-116. Unit IV – Page. No: 141-145, 150-155, 157-162 and 46-49. Unit V – Page. No: 195-199, 237-246 and 250-252. 2. William C. Frazier & Dennis C.Westhoff, (1996), Food Microbiology, Tata Mc Graw - Hill Publishing Company Ltd., Bombay, III Edition. 3. Rita Singh, (2004), Food Biotechnology, Volume I, Global Vision Publishing House, Delhi, I Edition. 4. Manorajan Kalia and Sangita Sood, (2008), Household food processing and preservation, Agrotech Publishing Academy, Udaipur. 5. Bharat singh, (2005), Food storage and preservation, Anmol Publications Pvt Ltd., New Delhi, I Edition.
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Page 130 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER III CORE COURSE HLBP31 - CELL AND MOLECULAR BIOLOGY (for those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: recall the structure and functions of cell and cell cycle. CO2: explain about the structure and functions of cell organelles. CO3: examine the different types of genome organization and gene expression. CO4: elaborate the steps involved in DNA cloning and DNA sequencing. CO5: discuss the process, types and applications of PCR and its allied techniques.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 1 - - - - - CO2 3 3 - - - - - CO3 3 3 3 - - - - CO4 9 9 3 1 - 1 - CO5 9 3 1 3 - - - Weightage of 27 19 7 4 - 1 - the course Weighted percentage of 4.55 4.7 2.43 2.92 0 20 0 Course contribution to POs
Unit – I (18 Hours) Cell Structure - prokaryotic and eukaryotic cell. Cell wall - ultra structure. Plasma membrane - molecular structure - chemical nature and functions. Mitochondria - origin and structure. Plastids - classification - types - structure and development of plastids. Cell cycle - mitosis and meiosis.
Unit – II (18 Hours) Structure and functions of (in brief) - ribosomes, dictyosomes, lysosome,
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Page 131 of 159 sphaerosome, glyoxysome, peroxisomes, nucleus and nucleolus. Chromosome - structure - chemical organization and special types - lampbrush and polytene.
Unit- III (18 Hours) Chloroplast and mitochondrial genome organization - prokaryotic and eukaryotic DNA differentiations. Regulation of gene expression in prokaryotes - lac Operon and trp operon - regulation of gene expression in eukaryotes. Post transcription modification of protein – 5 prime capping, 3 prime tailing and RNA splicing.
Unit- IV (18 Hours) DNA cloning – generation of sticky and blunt ends - homopolymeric tailing - use of linkers and adapters. Chimeric DNA formation, Probe construction -labeling - selection and screening of recombinants. DNA sequencing - principles of chemical and enzymatic methods. Site directed mutagenesis -cassette mutagenesis.
Unit –V (18 Hours) PCR- basic process, types and applications - PCR based molecular markers - RAPD, RFLP, minisatellites, microsatellites. Arbitrary primed polymerase chain reaction (AP-PCR) and AFLP. Applications of molecular markers in medicine and Forensic Science.
Reference books: 1. Verma P.S. and V.K.Agarwal (1985). Cytology, S.Chand Publications, Nagercoil, V Edition. Unit-I - Page No: 196-203,219-231,239-241 and 448-467. Unit II –Page No: 274-289,177-195,243-248,290-304 and 308-329 2. Gupta P.K. (2004). Biotechnology and Genomics, S. Chand & Company Ltd. New Delhi Unit-V - Page No: 61-69, 127-135, 350 and 371-372. 3. Kumaresan, V. (2010). Biotechnology, Saras Publication, Nagercoil, VI Edition Unit – IV- Page No: 176-178,182-185 and 294-305. 4. Verma, P.S. and V. K. Agarwal. (Reprint1999). Concept of Cell Biology, S. Chand Company LTD, New Delhi I Edition. Unit I - Page No: 37-42, 46-56 and 94-106. 5. Watson, J.D. (1987). Molecular Biology of Gene – The Benjamin / Cummings Publishing Company Inc., California, V Edition. Unit-III - Page No: 483-490 and 529-537. 6. De Robertis E.D.R and De Robertis E.M.R. (2012).Cell and MolecularBiology, Wolters Kluwer India.Pvt.Ltd. (VIII Edition).
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Page 132 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER III CORE COURSE HLBP32-ENVIRONMENTAL MICROBIOLOGY (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: relate the microbial interaction and plant- microbe interactions. CO2: illustrate the microbiology of air, soil and role of microbes in nutrient cycling. CO3: analyze microbial adaptations and associations in extreme environment. CO4: explain the various bioremediation technology. CO5: discuss the ways to solve the problems posed by solid and liquid waste.
CO-PO Mapping table (Course Articulation Matrix) POs PO1 PO2 PO3 PO4 PO5 PO6 PO7
COs CO1 9 - 3 - - - - CO2 9 3 3 - - - CO3 3 3 9 - - - - CO4 9 3 9 3 - - - CO5 9 9 9 3 - - - Weightage of 39 18 33 6 - - - the course Weighted percentage of 6.58 4.46 11.46 4.38 0 0 0 Course contribution to POs
Unit –I (18 Hours) Microbial adaptations in extreme environment - halophiles - thermophiles - acidophiles - alkaliphiles - barophiles. Microbial interactions - microbe-microbe- positive - cooperation - commensalism - synergism - mutualism - negative - competition- ammensalism - predation and parasitism. Plant microbe- spermosphere - rhizosphere - phyllosphere. Unit –II (18 Hours) Aerobiology - microbial contamination of air – sources – indicators - air sampling devices - air sanitation - droplet nuclei – aerosols - air borne transmission of microbes -
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Page 133 of 159 assessment of air quality. Aquatic habitats - freshwater - lakes - ponds and streams, marine habitats - estuaries, deep sea, coral reefs and mangroves and their microbial communities.
Unit –III (18 Hours) Soil Microbiology – Structure – types. Physical and Chemical properties - soil microbial sources. Biogeochemical cycles – nitrogen, carbon, phosphorus, sulphur, iron cycles and its significance.
Unit –IV (18 Hours) Wastewater microbiology - sewage – characteristics - dissolved oxygen - biochemical oxygen demand - chemical oxygen demand. Solid waste management - saccharification - pyrolysis - gasification - biogas – vermicompost. Biofuel -hydrogen.
Unit –V (18 Hours) Bioremediation - types - in-situ - intrinsic – engineered - ex-situ - solid phase - slurry phase - bioremediation of hydrocarbons - bioremediation of industrial wastes - dyes - paper and pulp industry. Heavy metals - metal microbe interaction. Bioaccumulation - biosorption. Xenobiotics - microbial degradation – bioaugmentation – biofiltration - microbial leaching - in-situ - ex-situ methods - copper and uranium.
Reference Books: 1. Rajan, S. and R. Selvi Christy Gupta. (2015). Essentials of Microbiology, Anjanaa Book House, Chennai. Unit- I - Page No: 309-313. Unit- II - Page No: 293-308,316-317. Unit- III - Page No: 251-269. Unit- IV - Page No: 332-340. Unit- V - Page No: 345-357. 2. Vijayan Ramesh, K. (2014). Environmental Microbiology, MJP publishers, Chennai. Unit- I- Page No: 206-228. Unit- III - Page No: 280-308. Unit- IV - Page No: 323-338. 3. Dubey, R.C. (Reprint 2013). A text book of Biotechnology, S.Chand & company Ltd, New Delhi. Unit- IV - Page No: 589-591. Unit- V - Page No: 570-589,592-594. 4. Sharma,P.D. (2014). Environmental Microbiology, Narosa Publishing House, New Delhi. 5. Pelczar, J.R., Chan, E.C.S., Krieg, R. (Reprint 1998). Microbiology, Tata Mc Graw- Hill Publishing Company Limited, New Delhi. 6. Dubey, R.C. and D.K.Maheswari. (Reprint 2012). Microbiology, S. Chand & Company Ltd, New Delhi.
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Page 134 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER III CORE COURSE HLBP33 - PLANT PHYSIOLOGY (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 04
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: demonstrate the absorption of water in land plants. CO2: explain the physiological process such as photosynthesis, respiration, photorespiration and circadian rhythm. CO3: explain the metabolism of lipid and nitrogen. CO4: analyze about plant growth regulators and seed dormancy. CO5: discuss about the various physiological mechanisms that protect the plants from environmental stress.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 3 3 - - - - CO2 9 9 3 1 - - - CO3 9 3 - - - - - CO4 3 9 3 1 1 - - CO5 9 9 3 1 1 - - Weight age of 33 33 12 3 2 - - the course Weighted percentage of 5.56 8.17 4.17 2.19 5.88 0 0 Course contribution to POs
Unit – I (18 Hours) Water relations in plants - physical phenomena - water potential and its components, apoplast - symplast - absorption of water - water absorbing organs - mechanism of water absorption - active absorption - passive absorption. Ascent of sap - path and mechanism - vital force theory, root pressure theory, physical force theory - Dixon’s cohesion theory. Transpiration - types - mechanism of stomatal movement - old and new hypothesis. Hydroponics, Aeroponics.
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Page 135 of 159 Unit – II (18 Hours) Photosynthesis - photosynthetic apparatus and functions. Mechanism of photosynthesis -light reaction - cyclic - non cyclic photophosphorylation Red drop -
Emerson enhancement effect. Carbon assimilation - C3 - C4 cycle and CAM pathway and its significance. Photorespiration.
Unit – III (18 Hours) Respiration - glycolysis, TCA cycle - electron transport system coupled with oxidative phosphorylation and inhibitors of electron transport system - ATP balance sheet calculation. Pentose phosphate pathway and its significance. Lipid metabolism - α and β oxidation of fatty acids - gluconeogenesis and glyoxylate cycle.
Unit – IV (18 Hours) Nitrogen metabolism -.nitrogen fixation - symbiotic nitrogen fixation - asymbiotic nitrogen fixation. Ammonia assimilation – reductive and transamination. Plant growth regulators - occurrence - physiological effects and practical applications of auxins - gibberellins - cytokinins - ABA and ethylene. Photoperiodism - role of phytochrome. Vernalisation. Seed dormancy and seed germination. Circadian rhythm.
Unit – V (18 Hours) Stress physiology - abiotic and biotic stress - causes of stress related to flooding, drought, saline, alkaline and UV radiation. Role of proline in stress physiology. Reference Books: 1. Pandey, S.N. and B.K.Sinha. (1988). Plant Physiology, Vani Educational Books, New Delhi, IV Edition. Unit I – Page No: 57-139 Unit II – Page No: 223-277 and 297-303. Unit III – Page No: 319-354 and 427-435. Unit IV – Page No: 361-373 and 525-537. Unit V – Page No: 538-549. 2. Verma, V. (Reprint 2007). Text Book of Plant Physiology, Anne Books India, New Delhi, Unit I – Page No: 147-186, 205-254. Unit III – Page No: 448-451. 3. Bidwell, R.G.S. (1979). Plant Physiology, McMillan publishers, New York, II Edition. Unit III – Page No: 108-138. Unit IV – Page No: 476-488, 504-509, 546-556 and 557-577. Unit V – Page No: 637-646. 4. Noggle, R. and G.I.Fritz. (1989). Introductory Plant physiology, Prentice Hall of India, New Delhi, II Edition. 5. Salisbury, F.B and E.Ross. (2010). Plant Physiology, Wadsworth, Belmont, California, IV Edition.
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Page 136 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER III CORE COURSE HLBP3L- PRACTICAL III (Cell and Molecular Biology, Environmental Microbiology and Plant Physiology) (for those admitted in June 2017 and later)
Contact hours per week : 2+2+2 Total number of hours per semester : 30+30+30 Number of credits 04
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: demonstrate skill in squash and smear techniques. CO2: make use of a protocol for the isolation of DNA and RNA. CO3: analyse the quantification of primary and secondary metabolites. CO4: evaluate the potability of water under different environmental conditions. CO5: estimate various nutrients in soil.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 3 1 - - - - CO2 9 9 3 - 3 - - CO3 3 9 9 1 - - - CO4 3 9 9 1 - - - CO5 3 3 3 - - - - Weightage of 27 33 25 2 3 - - the course Weighted percentage of 4.55 8.17 8.68 1.46 8.82 0 0 Course contribution to POs
Cell and Molecular Biology • Cytological techniques – mitosis and meiosis • Isolation and estimation of DNA. • Isolation and estimation of RNA. • PAGE and SDS-PAGE gel Electrophoresis.
Environmental Microbiology • Isolation of Rhizobium from root nodules. • Isolation of Cyanobacteria from soil.
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Page 137 of 159 • Determination of salinity - water sample • Determination of alkalinity - water sample • Determination of total hardness • Determination of sodium, calcium and potassium from soil sample – Flame Photometer. • Determination of nitrogen. • Dissolved Oxygen and Biological Oxygen Demand – Winkler’s method. • Chemical Oxygen Demand.
Plant Physiology • Determination of Diffusion Pressure Deficit (DPD) with potato tubers. • Calculation of stomatal index of upper and lower epidermal peelings of (Rheo leaf). • Estimation of water content, dry matter and ash content in higher plants. • Determination of osmotic potential of cell sap by plasmolytic method. • Quantitative estimation of photosynthetic pigments (chlorophyll a, b, total chlorophyll and carotenoids) by spectrophotometry. • Determination of absorption spectrum of chlorophyll. • Quantification of carotenes and xanthophylls by solvent fractionation method. • Estimation of anthocyanin content in leaves and petals. • Estimation of free amino acids in plant tissue by ninhydrin method. • Estimation of proline. • Estimation of in vivo nitrate reductase activity in higher plants. • Estimation of ascorbic acid in plants. • Seed viability test. Spot at sight • Electron micrographs of cell organelles. • Carbon cycle. • Nitrogen cycle. • Phosphorous cycle. • Sulphur cycle. • Iron cycle. • Biogas. • Vermicompost. • Bioremediation. • Xenobiotics. • Bioaugmentation. • Bioleaching.
Submission of record.
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Page 138 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY SEMESTER III ELECTIVE COURSE HLBP3E1/HLMBP3E3 – CSIR UGC-NET Preparatory course - Life Sciences (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 No. of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: explain the basic principle of biochemistry and molecular biology. CO2: analyse insights of cell functions and embryo development. CO3: assess the importance of plant and animal physiology. CO4: compose the genetic variations and outline the hierarchy of diversity forms. CO5: apply and utilize the advanced techniques in the biomedical field.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 1 ------CO2 3 3 - - - - - CO3 9 3 3 - - - - CO4 9 9 3 3 - - - CO5 9 9 9 3 - - - Weightage of 31 24 15 6 - - - the course Weighted percentage of 5.23 5.94 5.21 4.38 0 0 0 Course contribution to POs
Unit I - Biochemistry and Molecular Biology (18 hours) Principles of Biochemistry - pH, Buffer, enzyme kinetics, inhibition kinetics Aquaporins- water channels. Concept of gene-DNA replication, transcription factors, protein synthesis, Post translational modification of protein, Purification of protein. Transposons. Regulation of gene expression - Lac operon, Trp operon. Control of expression of Aqrobacterium vir genes. Role of chromatin in regulating gene expression. Gene silencing. DNA supercoiling. DNA sequencing.
Unit II - Cytology and Developmental biology (18 hours) Cell communication, Host parasite interaction and Neuro transmission. Cell signalling- Signal reception,signal transduction and cellular response. Quorum sensing. Cell cycle. Intracellular Receptors.Genes of cancer.
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Page 139 of 159 Embryogenesis and floral development in Arabidopsis. Caehorhaditis elegans as a model organism. Axis and pattern formation in Drosophila and amphibians. Limb development in vertebrates.
Unit III - Physiology (18 hours) Plant physiology - Secondary metabolites in plants - Terpenes, Alkaloids, Glycosides and Phenolics. Stress physiology-Water, Temperature and Oxidative stress. Mechanism of loading & unloading of photo assimilates. Carbon isotopes ratio of C3 and C4 plants Animal physiology-Respiratory and excretory systems. physiology of visions, Cardiac action potential. Stress and adaptations, High altitude problems.
Unit IV – Genetics, Diversity of Life forms and Ecology (18 hours) Extensions of Mendelian principles –Interaction of genes, mutation, linkage and crossing over, Blood grouping, cytoplasmic inheritance, sex linked inheritance. Pedigree Analysis. Levels of structural organization - unicellular, colonial and multicellular forms. Angiosperm Taxonomy and cladistics. Ecological principles-Shanon’s and Simpsons diversity index. Population ecology. Biogeography - terrestial biomes, Island biogeography. Biogeographical zones of India. Outlines of conservation strategies.
Unit V - Applied Biology and Evolution (18 hours) Biophysical methods - Analysis of biomolecules using Electron microscope, NMR. Electrophysiological methods - ECG, PET, MRI. Methods in field Biology - Remote sensing. Population genetics-Populations, gene pool, gene frequency, Hardy-Weinberg law. genetic drift, continental drift and Evolution. Mating systems.
Reference Books: Unit I 1. Lehninger. (2008). Principles of Biochemistry, W. H. Freeman & Company, 52nd Edition. 2. Harvey Lodish, Arnold Berk, Anthony Bretscher, Chris A. Kaiser, Hidde Ploegh, Matthew P. Scott, Paul Matsudaira, Monty Krieger. (2007). Molecular cell biology, W. H. Freeman & Company, 6th Edition. 3. Kumar Pushkar and A.P.Singh.(2014), CSIR-UGC NET/JRF/SET Life Sciences, Upkar Prakashan, Agra. ISBN : 978-81-7482-423-3 4. Keith Wilson and John Walker, (2010), Principles and Techniques of Biochemistry and Molecular Biology, 7th Edition. Unit II 1. Gerald Karp, (2013). Cell and Molecular Biology, Wiley publication, 7th Edition Binder Ready Version edition. 2. Scott F. Gilbert. (2013). Developmental biology, Scott F. Gilbert Publishers, 10th Edition Unit III 1. Lincoln Taiz, Eduardo Zeiger, Ian M. Møller, and Angus Murphy. (2006). Plant Physiology and Development, Sinauer Associates Publishers, Sixth Edition.
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Page 140 of 159 2. Kent M. Van De Graaff, R. Ward Rhees. (2001). Schaum's Easy Outline of Human Anatomy and Physiology, McGraw Hill Professional publishers. Unit IV 1. Benjamin A. Pierce. (2010). Genetics: A Conceptual Approach, Mcmillan publication, Fourth Edition. 2. V. N. Naik, (1984), Taxonomy of Angiosperms, Tata McGraw-Hill Education, 1st Edition. 3. Lansing M. Prescott, John P. Harley, Donald A. Klein, (2002). Prescott's Microbiology, McGraw-Hill publication, 5th Edition. 4. Peter Stiling. (1999). Ecology: Theories and Applications, Prentice Hall publishers, 1st Edition. Unit V 1. John Maynard Smith. (1993). The theory of evolution, Cambridge University Press, 1st Edition. 2. Kalyan Kumar D. (2008). An Introduction to Plant Tissue Culture, New Central Book Agency (P) Ltd. , 7th Edition 3. T. A Brown. (2010). Gene Cloning and DNA Analysis: An Introduction, Wiley Publications, 6th Edition.
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Page 141 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER III ELECTIVE COURSE HLBP3E2 -ENERGY RESOURCES (for those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1 : list out the energy requirement in relation to population and industrial growth. CO2 : summarize the source and applications of non conventional energy resources. CO3 : make use of solar energy and solar gadgets for thermal conversion. CO4 : classify the basic components of wind energy generating system and management of wind forms. CO5 : analyze the production and use of bioenergy.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 1 ------CO2 3 3 - - - - - CO3 9 3 3 - - - - CO4 9 9 3 3 - - - CO5 9 9 9 3 - - - Weightage of 31 24 15 6 - - - the course Weighted percentage of 5.23 5.94 5.21 4.38 0 0 0 Course contribution to POs
Unit – I (18 Hours) Definition, introduction to conventional energy sources. Nonrenewable forms – fossil fuels and its impact, present energy scenario. Energy crises. Available energy sources for future – renewable energy sources. Hydro power and wood. Energy requirement in relation to population and industrial growth, need to tap more energy from various non conventional sources.
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Page 142 of 159 Unit – II (18 Hours) Non conventional energy sources – source, mechanism, and applications of ocean energy - tidal energy - wave energy - thermal energy, geothermal energy. Hydrogen fuel. Biofuels. Improved cook stove for villagers.
Unit – III (18 Hours) Solar energy - photo thermal conversion. Solar collectors - concentrating collectors. Solar photovoltaic conversion. Solar energy utilization in India. Solar gadgets - solar cooker, solar thermal engine, solar desalination. Future prospects for solar energy.
Unit – IV (18Hours) Wind energy - basic components of a wind energy generating system (Wind mill) - transmission and control - supporting structure - wind power details. Advantages and disadvantages of wind energy system. Wind power development in India. Management of wind farms. Future prospects of wind energy.
Unit – V (18 Hours) Biomass - fermentation, bioenergy. Substrate available for biogas production - factors affecting biogas production. Various models of biogas plants. Biogas from various waste materials including night soil. Recent advances in biogas generation and its application in rural sectors. Vegetable oils and alcohols as energy sources.
Reference Books: 1. Koteswara Rao, M.V.R., (2004), Energy resources, Conventional and Non Conventional, BS publications, Hyderabad, I Edition. 2. Shyam M., Pandey K.C., and A.K. Dubey, (reprint 2005), Renewable energy Technologies for rural sector, Published by Central Institute of Agricultural Engineering, Nabi bagh, Berasia Road, Bhopal. 3. Shyam M., (reprint 2003), Biennial Report on Renewable Energy Sources, Published by Co-ordinating cell on RFS, CIAE, Bhopal, Madhya Pradesh. 4. Welford, R., (reprint 1996), Comporate Environment Management, Univ. Press, Hyderabad.
Study material will be provided.
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Page 143 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER IV CORE COURSE HLBP41-PLANT ECOLOGY (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learner should be able to CO1: outline the basics of ecology, concept of ecosystem and ecological succession. CO2: analyze the biodiversity and methods of studying plant community. CO3: discuss about environmental pollution and disaster management. CO4: summarize about phytogeography. CO5: explain environmental impact assessment and role of GOs and NGOs in its management.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 3 - 1 - - - - CO2 9 1 - - - - - CO3 9 3 3 3 - 1 - CO4 3 3 - 1 - - - CO5 9 1 - 1 - - - Weightage of 33 8 4 5 - 1 - the course Weighted percentage of 5.56 1.98 1.39 3.65 0 20 0 Course contribution to POss
Unit-I (18 Hours) History and scope of Ecology. Concept of ecosystem. Energy flow - single channel, Y-shape. Ecological succession -causes, types - primary, secondary, autogenic, allogenic autotrophic and heterotrophic succession, general process of succession - nudation, biotic, invasion, migration, ecesis, aggregation. Climax concept in succession - monoclimax and polyclimax and climax pattern hypothesis - hydrosere, lithosere.
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Page 144 of 159 Unit-II (18 Hours) Methods of studying plant community – quadrat, transect- line and belt, point frame method and loop method. Invasive and keystone species. Biodiversity - definition, scope and constrains of biodiversity, Vavilov’s principles, current levels of biodiversity, organization in diversity. , and biodiversity. Types of biodiversity - Species, genetic and habitat biodiversity. Hot spots of biodiversity. Disaster management - floods, earthquake, cyclone, Tsunami and landslides.
Unit III (18 Hours) Environmental pollution - definition, pollutants and types, sources, causes, effect and control measures of the following : Air pollution - (ozone depletion, acid rain and green house effect), water pollution, noise pollution, radioactive pollution, thermal pollution and marine pollution.
Unit-IV (18 Hours) Principles of Phytogeography. Continental drift. IUCN. Red data book. Formation of endemics - Theories and causes of endemism. Categories of endemism - relic endemics, paleo endemics, neo- endemics, pseudoendemics. Sacred species and sacred grooves. Remote sensing and geographic information system (a brief account).
Unit-V (18 Hours) Environmental impact assessment- development - projects covered, procedure for environmental impact assessment, environmental appraisal committee - environmental management plan and industrial projects. Environmental management. Environmental education and organizations (a brief account).
Reference Books: 1. Odum, E. P. and Barrett. (2005). Fundamentals of Ecology, Cengage Learning India private Limited, V Edition. Delhi. 2. Sharma, P.D. (2012). Ecology and Environment. Rastogi Publications, Meerut. X Edition. Unit I - Page No: 3-17, 146-161 and 175-181 Unit II - Page No: 136-144 Unit V - Page No: 579-612 3. Shukla, R.S. and P.S.Chandel. (1972). Plant Ecology- S.Chand & Company, (First edition). New Delhi. Unit II - Page No: 110-140 Unit IV - Page No: 215-220 4. Saha T.K. (Reprint 2011). Ecology and Environmental biology- Books and Allied Private Limited, Maharashtra. Unit IV - Page No: 398-401and 515-518 5. S.S.Purohit, Q.J.Shammi and A.K.Agarwal. (2004). A text book of Environmental sciences, Student edition, Jodhpur. Unit III - Page No: 207-247 and 257-289 Approved in the Academic Council meeting held on 13.06.2019 PGBOT -Page 57
Page 145 of 159 6. Misra, S.P. and S.N.Pandey. (2011). Essential of Environmental studies. Ane Books Pvt.Ltd, Third edition New Delhi. Unit II - Page No: 282-298 and 484-503 7. S.C. Santra. (2016). Environmental Science, New central book agency (P) Ltd., Kolkata. Unit IV - Page No: 562-568. Unit II- Invasive species https://www.nwf.org/Educational-Resources/Wildlife-Guide/Threats-to- Wildlife/Invasive-Species http://hydrodictyon.eeb.uconn.edu/courses/introconsbio/EEB208_11_invasives_2.htm Unit II- Keystone species https://www.nature.com/scitable/knowledge/library/keystone- species-15786127 http://examples.yourdictionary.com/examples-of-keystone-species.html
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Page 146 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER IV CORE COURSE HLBP42- APPLIED BIOTECHNOLOGY (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: recall the core concepts and fundamentals of plant Biotechnology, biological nitrogen fixation and genetic engineering. CO2: develop their ability to raise plantlets on different types of plant tissue culture. CO3: list the different types of biofertilizers based on their mass cultivation and its importance. CO4: compare the various types of fermentation and produce the various industrial products. CO5: compile fundamental knowledge on bioinformatics and problems and its solution in bioethics.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 1 - 3 - - - - CO2 - 3 9 3 3 - - CO3 9 3 3 1 3 - - CO4 9 3 3 - - - - CO5 1 3 3 - 3 - - Weightage of 20 12 21 4 9 - - the course Weighted percentage of 3.37 2.97 7.29 2.92 26.47 0 0 Course contribution to POs
Unit-I (18 Hours) Introduction - definition, scope of Plant Biotechnology, organizations of Plant Biotechnology in India and applications of biotechnology. Role of Agrobacterium in Plant Biotechnology. Ti based plasmids - construction, insertion of cDNA into plant DNA. Biological nitrogen fixation - symbiotic nitrogen fixation - establishment of symbiosis, factors affecting nodule formation, mechanism of nitrogen fixation in root
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Page 147 of 159 nodules. Nif gene - cloning of nif gene. Transgenic plants - Bt cotton, virus resistance, herbicide resistance and drought resistance (a brief account).
Unit-II (18 Hours) Plant tissue culture – principles. Types of media- MS and B5 medium, explant, organogenesis, cell culture, somatic embryogenesis. Protoplast culture - Isolation, sterilization, purification, regeneration, fusion and somatic hybridization - methods of somatic hybridization, selection of somatic hybrids and cybrids. Production of haploid plants – androgenesis, gynogenesis. Applications and limitations of haploids. DNA transfer methods - microinjection, liposome mediated transformation, ultrasonication, shotgun method and agroinfection.
Unit-III (18 Hours) Biofertilizer - definition, importance. Algal biofertilizer - mass cultivation and significance of BGA and Azolla. Bacterial biofertilizer - mass cultivation and significance of Rhizobium and Azospirillum. Mycorrhizal fungi - mass cultivation and application of VAM. Role of leguminous plants as green manure.
Unit-IV (18 Hours) Fermentation technology – batch fermentation, fed-batch fermentation, continous fermentation, product recovery. Industrial production of ethanol, lactic acid and streptomycin. Production of amylase and insulin. Production of SCP- Spirulina.
Unit-V (18 Hours) Basic fundamentals of Bioinformatics - scope and applications. Genomics and proteomics database. Sequence data base- NCBI / PIR, BLAST and FASTA. Bioethics – problems, solutions and its significance. Intellectual property rights (IPR) and patenting.
Reference Books: 1. Dubey, R.C. (2012). Text book of Biotechnology, S. Chand and Company Ltd, New Delhi. IV Edition. Unit - I - Page No: 1-24 and 319-328 Unit- II - Page No: 280-312. Unit- III - Page No: 463-486 and 498-503. Unit- IV - Page No: 511-514 and 559-563. Unit- V - Page No: 204-217 and 605-614 2. Kumaresan, V. (2010). Biotechnology, Saras publication, Nagercoil, VI Edition. Unit - V - Page No: 392-395. Unit - II - Page No: 435-485 3. Ramawat, K.G. (2004). Plant Biotechnology, S. Chand and Company Ltd, New Delhi. II Edition. Unit- II - Page No: 37-47, 61-65 and 169-174. 4. Satyanarayana, U. (Reprint 2010). Biotechnology, Books and allied Pvt. Ltd, Chennai. Unit- I - Page No: 573-561 Unit- II - Page No: 497 – 545 and 583-587. Unit- IV - Page No: 312-338 and 259-263.
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Page 148 of 159 5. S.S.Purohit, S.K.mathur. (1996). Biotechnology Fundamentals and applications, Agro Botanical Publishers, India. Unit- I - Page No: 44-48 and 59-73 6. Razdan M.K. (2003). Introduction to Plant tissue culture, Science Publishers, New Delhi. II Edition.
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Page 149 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER IV CORE COURSE HLBP4L – PRACTICAL IV (Plant Ecology and Applied Biotechnology) (For those admitted in June 2017 and later)
Contact hours per week : 3+3 Total number of hours per semester : 45+45 Number of credits 04
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: analyze the nature of vegetation. CO2: examine the primary productivity. CO3: evaluate the role of biofertilizers on plant growth. CO4: demonstrate various tissue culture techniques. CO5: compare the protein and nucleotide sequence in different plants.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 1 - 3 - - - - CO2 9 3 9 3 3 - - CO3 9 3 3 1 3 - - CO4 - 3 3 - - - - CO5 1 3 3 - 3 - - Weightage of 20 12 21 4 9 - - the course Weighted percentage of 3.37 2.97 7.29 2.92 26.47 0 0 Course contribution to POs
Plant Ecology • Permanent quadrat. • Quadrat method. • List quadrat • Line transect. • Belt transect. • Point frame method. • Species area curve method. • Zig- Zag transect method.
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Page 150 of 159 • Random sampling method • Frequency index. • Frequency curve. • Importance value index. • Measurement of primary productivity. Environmental pollution Air pollution • ozone depletion. • acid rain. • green house effect. Water pollution • pesticide. • herbicide. • biomagnification.
Energy flow • single channel. • Y-shape model. Hotspots of India. Sacred species and grooves.
Applied Biotechnology • Sterilization techniques.
• Preparation of tissue culture medium (MS medium) and stock solution.
• Preparation of artificial seed.
• Callus culture, embryo culture, anther culture.
• Effect of biofertilizers on plant growth.
• Isolation and screening of enzyme producing microorganisms.
• Precipitation of crude amylase by ammonium sulphate method.
• Biosorption of metallic pollutants using immobilized application.
• Analysis of nucleotide and protein sequence using BLAST.
• Analysis of nucleotide and protein sequence using FASTA.
• Multiple sequence alignment.
• Protein secondary structure prediction.
• Analysis of expressed sequence tags using NCBI database.
• Handling EMBOSS tools using biological sequence.
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Page 151 of 159 Spotters • Ti plasmid. • Nif gene. • Bt cotton. • Callus. • Azolla. • SCP. • VAM. • Ethanol production. DNA transfer methods • microinjection, • liposome mediated transformation. • Ultrasonication. • shotgun method.
Submission of record notebook.
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Page 152 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY SEMESTER IV ELECTIVE COURSE HLBP4E1 – BIOPHYSICS AND BIOSTATISTICS (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05 Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: summarize bioenergetics, photobiology and concepts of biostatistics. CO2: determine the measures of central tendency and measures of dispersion. CO3: analyze about correlation and regression. CO4: relate the knowledge on probability and theoretical distribution. CO5: formulate hypothesis and test the significance with interpretation.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 3 1 - - - - CO2 3 9 9 3 - - - CO3 3 9 3 3 1 - - CO4 3 3 - - - - - CO5 9 9 9 3 1 - - Weight age of 27 33 22 9 2 - - the course Weighted percentage of 4.55 8.17 7.64 6.57 5.88 0 0 Course contribution to POs
Unit – I (18 Hours) Bioenergetics - laws of thermodynamics - Concept of free energy - redox potential - ATP as a high energy compound - chloroplast and mitochondrial bioenergetics. Photobiology - nature of light - solar radiation - properties of light. Biological energy conversion - fluorescence - phosphorescence - bioluminescence and its significance.
Unit – II (18 Hours) Basic concepts of biostatistics. Collection, processing - classification – tabulation, diagrammatic presentation and graphic presentation of data. Measures of central tendency
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Page 153 of 159 and their merits and demerits - mean, median and mode - their relationship - simple problems
Unit – III (18 Hours) Measures of dispersion - range - quartile deviation - mean deviation - coefficient of variation - standard deviation and standard error - simple problems. Probability – measurent – laws - theorems and applications. Theoretical distribution - binomial - poisson - normal distribution and ‘t’ distribution. Sampling - definition - types - sampling techniques and sampling error.
Unit – IV (18 Hours) Correlation - introduction - types - positive - negative - simple - partial - linear and non-linear - methods of studying correlation - scatter diagram and graphic methods - Karl Pearson’s co-efficient correlation - simple problems. Regression - equation. Non linear and multiple regressions. Uses of regression.
Unit – V (18 Hours) Tests of significance - chi - square test,‘t’ – test. Null and alternate hypothesis. Analysis of variance - F-test - one way ANOVA and two way ANOVA. Simple problems.
Reference Books: 1. Salil Bose, S. (1982).Elementary Biophysics and Biostatistics, Vijaya Printers, Madurai. Unit I - Page No: 17-51, 69-80 and 96-99 2. Arumugam, N. and V. Kumaresan. (2015). Principles of Techniques of Biophysics, Saras Publication, Nagercoil. Second Edition. Unit I - Page No: 54-123. 3. Khan, H and A. Khanum. (Reprint 2009). Fundamentals of Biostatistics, Ukaaz publications, Hyderabad. III Edition. Unit II- Page No: 49-76 Unit III - Page No: 08-127, 161-189 and 8-15 Unit IV - Page No: 204-227. Unit V - Page No: 262-283. 4. Ramakrishnan, P. (Reprint 2000). Biostatistics, Saras Publication, Nagercoil. Unit II - Page No: 5-113 and 114-157. Unit V - Page No: 334 – 361, 303-304 and 325-331. 5. Sundar Rao, P.S.S and J. Richard, (2005). Introduction to Biostatistics and Research methods, Prentice Hall of India private Ltd, New Delhi, IV Edition. Unit III - Page No: 31-65 Unit IV - Page No: 89-93. Unit V - Page No:94-101
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Page 154 of 159 6. Gurumani, N. (impressions 2010). An Introduction to Biostatistics, MJP publishers, Chennai. Unit III - Page No: 78-88 Unit V - Page No: 228 7. Manoharan, M. and S.Palanichamy. (Reprint 2008). Statistical methods, Palani Paramount Publications, Palani. III Edition. 8. Unit V- F test - https://people.richland.edu/james/lecture/m170/ch13-f.html
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Page 155 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY SEMESTER IV ELECTIVE COURSE HLBP4E2 – MICROBIAL GENETICS (For those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: recall the effect of mutation on gene function. CO2: analyse about genetic recombination in T4 phage. CO3: distinguish DNA and RNA virus genomes and its role in molecular genetics. CO4: explain the chromosomal and extra chromosomal inheritance in yeast. CO5: demonstrate the genetics of bacteria and bacteria phages.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 3 1 - - - - CO2 3 9 9 3 - - - CO3 3 9 3 3 1 - - CO4 9 3 - - - - - CO5 3 9 9 3 1 - - Weightage of 27 33 22 9 2 - - the course Weighted percentage of 4.55 8.17 7.64 6.57 5.88 0 0 Course contribution to POs
Unit –I (18 Hours) Genetic nomenclature, mutants – types, isolation and characterization. Genetic analysis, genetic recombination, genetic mapping, Linkage and multifactor crosses, deletion mapping, complementation, need for isogenic strains for genetic analysis.
Unit-II (18 Hours) Genome of virus - molecular genetics-RNA virus genomes – positive strand RNA virus, negative strand RNA viruses. DNA virus genomes –small DNA genomes, large DNA genomes, herpes virus genomes, adenovirus genomes, segmented and multipartite virus genomes.
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Page 156 of 159 Unit-III (18 Hours) Yeast genetics- cell structure, lifecycle, cell cycle, sexual reproduction –mating type A and alpha. Yeast genetic nomenclature, chromosomal inheritance, random spore analysis of tetrad analysis, extra chromosomal inheritance. Yeast genomics.
Unit-IV (18 Hours) Genetics of bacteria-plasmids –types, detection, purification and transfer of plasmid DNA, invitro plasmid transfer, replication and properties of F plasmids, drug-resistance plasmids, Agrobacterium plasmid Ti.
Unit-V (18 Hours) Genetics of phage T4-phage mutants, genetic recombination in phage, genetic mapping of phage T4,lytic growth of phage , lysogency and prophage integration prophage excision, decision between lysis & lysogency, dilysogens, role of in screening carcinogen.
Reference Books: 1. Rajapandiyan K.and S.Shanthi. (2011).Molecular biology and Microbial genetics, PBS Book Enterprises, Chennai. Unit – II Page No: 243-256 Unit – III Page No: 231-243 2. Stanly. R. Maloy. John. E. Cronan and David Friefelder. (2006). Microbial Genetics, Narosa Publishing House, New Delhi. II Edition Unit – I Page No: 1-26 Unit – IV Page No: 237-263 Unit – V Page No: 346-473 3. James D.Watson. (2007). Molecular Biology of the gene, Dorling Kindersley Pvt.Ltd., India. V edition 4. David Friefelder. (XIV Reprint 2005).Molecular Biology, Narosa Publishing House, New Delhi.
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Page 157 of 159 THE STANDARD FIREWORKS RAJARATNAM COLLEGE FOR WOMEN, SIVAKASI. DEPARTMENT OF BOTANY M.Sc. BOTANY WITH SPECIALIZATION IN PLANT BIOTECHNOLOGY SEMESTER IV CORE COURSE HLBP4P- PROJECT AND VIVA VOCE (for those admitted in June 2017 and later)
Contact hours per week 06 Total number of hours per semester 90 Number of credits 05
Course Outcomes (CO): On successful completion of the course, the learners should be able to CO1: develop laboratory skills and master in advanced techniques. CO2: make use of advanced tools in analyzing data. CO3: summarize the concept of research with ethics. CO4: defend their research professionally. CO5: justify valuable solutions to the betterment of society.
CO-PO Mapping table (Course Articulation Matrix) POs PO7 PO1 PO2 PO3 PO4 PO5 PO6 COs CO1 9 3 3 - 1 - - CO2 9 3 9 3 - - - CO3 9 - 9 3 - 3 - CO4 9 3 3 9 - - - CO5 9 9 3 9 - - 3 Weightage of 45 18 27 24 1 3 3 the course Weighted percentage of 7.59 4.46 9.38 17.52 2.94 60 75 Course contribution to POs
Rules Regarding Project and Project viva 1. Project can be done individually (or) in a group consisting of two. 2. The dissertation can be on • A Research Problem. • An advanced study of a topic in their syllabus. • Extensive study of an experiment. 3. The dissertation should be submitted to the Controller of Examination through the Head of the department one week prior to the commencement of End semesterExamination. 4. Each student shall submit 2 copies of her dissertation for valuation. 5. The project report shall contain at least 25 pages excluding bibliography and appendices.
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Page 158 of 159 The project work will be valued as • Report present – 80 • Viva voce – 20
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Page 159 of 159