1 5 10 20 30 40 50 60 70 iiii i i i i i i i i ii i notched-fin eelpout-Q1 mksviltgllfvllcvdhmssaNQ-ESVVAAVLIPINTAL TVGMMTTRVVSPTGIPAEDIPRLISMQVNQAVPMGTTLMPDMVKFYCLCAPKN viviparous-eelpout-Q1† mksviltgllfvllcvdhmssaNQ-ESVVAAVLIPINTAL TVGMMTTRVVSPTGIPAEDIPRLVSMQVNQAVPMGTTLMPDMVKFYCLCAPKN ocean pout-Q1* ------cvdhmssaNQ-ESVVAATLIPINTAL TVGMMTTRVVTPVGIPAEDIPRLVSMQVNQAVPMGTTLMPDMVKFYCLCAPKN ocean pout-Q2 mksviltgllfvllcvdhmssaNQ-ESVVATQLIPINTAL TLVMMTTRVIYPTGIPAEDIPRLVSMQVNQAVPMGTTLMPDMVKFYCLCAPKN Atlantic wolffish-Q1 mksailtgllfvllcvdhmssaHQ-ASVVATQLIPINTAL TLVMMTTRVVFPTGIPAEDIPRLVSMQVNRAVPMGTTLMPDMVKFYCLCAPKTQRW Atlantic wolffish-Q2 ---ailtgllfvllcvdhmssaNQ-ASVVATQLIPINTAL TLVMMTTAVVTPTGIPAEDIPRLVSMQVNRAVPMGTTLMPDMVKFYCLCAPKY Alaskan ronquil-Q1* ------nmssaK-GDSVVATQLIPINTAL TLVMMGTADVTPTGIPAEDIPRLVGMQVNRAVLMGTTLMPDMVKFYCLC ocean pout-Q3 mksviltgllfvllcvdhmssaNQ-ESVVATQLIPINTAL TLVMMTTRVIYPTGIPAEDIPRLVSMQVNQAVPMGTTLLPDMVKGYPL-T notched-fin eelpout-Q2 mksviltglffvllcvdhmssaNQ-ESVVAAVLIPINTAL TVGMMTTRVVSPTGIPAEDIPRLISMQVNQVVPMGTTLMPDMVKGYAP-A viviparous eelpout-Q2† mksviltglffvllcvnhmssaNQ-ESVVAAVLIPINTAL TVGMMTTRVVSPTGIPAEDIPRLISMQVNQVVPMGTTLMPDMVKGYAP-A ocean pout-Q4* ------cvdhmssaNQ-ASVVATQLIPINTAL TLVMMRSKVVTPMGIPAEDIPRLVSMQVNQAVACGTTLMPGMVKTYTP-AK viviparous eelpout-Q3† mksviltgllfvllcvdhmssaNQ-ASVVATQLIPINTAL TLVMMKAKVVTPMGIPAEDIPRIVSMQVNQAVACGTTLMPGMVKTYTP-VK notched-fin eelpout-Q3 mksviltgllfvllcvdhmssaNQ-ASVVATQLIPINTAL TLVMMKAKVATPMGIPAEDIPRIVSMQVNQAVACGTTLMPGMVKTYTP-VK ocean pout-Q5* ------cvdhmssaNQ-ASVVANQLIPINTAL TLVMMRSEVVTPVGIPAEDIPRLVSMQVNRAVPLGTTLMPDMVKGYPP-A radiated shanny-Q1* mnsviftglvfvllcvdnmssaASGQSVVANQLIPINTAL TLVMMRAEVVSPLGIPAEDIPRLVSLQVNRAVPLGTTLTAEMVKGYSP-AK rock gunnel-Q1* mnsailtgflfvllcvdnmtsaGSGKSVVANQLIPINTAL TRIMMKAELVAPMGIPAEDIPRLVSLQVNRAVPMGTTLMPDMVKTYQP-AK viviparous eelpout-Q4† mksviltgllfvllcvdhmssaNQ-ASVVANQLIPINTAL TLVMMRSEVVTPMGIPAVDIPRLVSMQVNRAVPLGTTLMPDMVKGYTP-A notched-fin eelpout-Q4 mksviltgllfvllcvdhmssaNQ-ASVVANQLIPINTAL TLVMMRAEVVTPMGIPAVDIPRLVSMQVNRAVPLGTTLMPEMVKGYTP-A viviparous-eelpout-Q5† mksviltgllfvllcvdhmssaDQ-ASVVANQLIPINTAL TLVMMRAEVVTPMGIPAEDIPRLVSLQVNRAVPLGTTIMPDMVKGYAP-N Canadian eelpout-Q1 ------NK-ASVVANQLIPINTAL TLVMMRAEVVTPAGIPAEDIPRLVGLQVNRAVLIGTTLMPDMVKGYAP-Q spotted wolffish-Q1 mksailtgllfvllcvdhmssaHQ-ASIVANQLIPINTAL TLI MMRAQVVTPLGIPAEDIPRIIGMQVNRAVALGTTLMPDMVKGYPP-N spotted wolffish-Q2 mksailtgllfvllcvdhmssaHQ-ASIVANQLIPINTAL TPI MTKAQVVTPLGIPAEDIPRIIGMQVNRAVALGTTLMPDMVKGYPP-N P. brachycephalum-1† mksvvltgllfvllcvdhmssaNK-ASVVANQLIPINTAL TLVMMKAEVVTPMGIPAEDIPRLIGMQVNRAVPLGTTLMPDMVKMYCLCI Antarctic eelpout-Q1 mksvvltgllfvllcvdhmssaNK-ASVVANQLIPINTAL TLI MMKAEVVTPMGIPAEDIPRIIGMQVNRAVPLGTTLMPDMVKNY---EK Antarctic eelpout-Q2 mksvvltgllfvllcvdhmssaNK-ASVVANQLIPINTAL TLI MMKAEVVTPMGIPAEDIPKLMGMQVNRAVPLGTTLMPDMVKNY---EK Antarctic eelpout-Q3a mksvvltgllfvllcvdhmssaNK-ASVVANQLIPINTAL TLI MMKAEVVTPMGIPAEEIPNLVGMQVNRAVPLGTTLMPDMVKNY---ED Antarctic eelpout-Q4 mksvvltgllfvllcvdhmssaNK-ASVVANQLIPINTAL TLVMMKAEVVTPMGIPAEEIPKLVGMQVNRAVPLGTTLMPDMVKNY---EK Antarctic eelpout-Q3b (tandemer) GTTSPGLKSVVANQLIPINTAL TLVMMKAEEVSPKGIPSEEISKLVGMQVNRAVPLGTTLMPDMVKNY---EK P. brachycephalum-Q2† mksvvltgllfvllcvdhmssaNK--SVVANQLIPINTAL TLVMMKAEEVSPKGIPAEEIPRLVGMQVNRAVPLGTTLMPDMVKNY---QK Antarctic eelpout-Q5b (tandemer) VTTCPGFKSAVANQLIPINTAL TLM MMKAEEVSPKGIPAEEIPKLVGMQVNRAVYLDQTLMPDMVKNY---ED Antarctic eelpout-Q6 ------SVVANQLIPINTAL TLVMMKAKEVSPKGIPAEEIPKLVGMQVNRAVYLDETLMPDMVKNY---EK P. brachycephalum-Q3 ------TK--SVVANQLIPINTAL TLVMMKAEEVSPKGIPAEEIPRLVGMQVNRAVYLDETLMPDMVKNY---E P. brachycephalum-Q4 mksvvltgllfvllcvdhmssaTK--SVVASQLIPINTAL TPAMMKAKEVSPKGIPAEEMSKIVGMQVNRAVNLDETLMPDMVKTY---QK ocean pout-Q6† ------cvdhmssaNQ-ASVVATQLIPINTAL TLVMMKAKVVTPMGIPAEEIPQIVGLQVDRAVPLGTTLMPDMVKTYCA-AK ocean pout-Q7 mksviltglflvllcvdhmssaNQ-ESVVATQLIPINTAL TPI MMKGKVVTPMGIPFKEMSQIVGKQVNRAVPLGTTIMPEMVKGYAP-N Antarctic eelpout-sasB ------GKSVVAKVKIPKGTVL TQDMLAVKAAEPMGIAAEDLCKMVGKTVTEDVEEDDSVMPEMVKGYCK-NKKC wolf eel-sasB ------GKSVVAKVKIPKGTVL TQDMLAVKAAEPMGIAAEDLCKMVGKTVTEDVEEDDSIMPEMVKGYCK-NKKC Antarctic eelpout-sasA ------GKSLVATVKIPKGTVL TQDMLTVKVAEPMGVAAEDIFQMVGKTVTKDVEEDGSLLPEVVDGYCK-KRKC wolf eel-sasA ------GKSLVAKVKIPKGTVL TQDMLTVKVAEPMGIAAEDIFQMVGKTVTKDVEEDDSLLPEVVDGYCK-KRKC Atlantic wolffish-S1 mksailtgllfvllcvdhmssaSQ--SVVATQLIPINTAL TPI MMKGKVVNPAGIPFAEMSQIVGKQVNRPVAKDETLMPNMVKTYRA-AK spotted wolffish-S1 mksailtgllfvllcvdhmssaSQ--SVVATQLIPINTAL TPI MMKGKVVNPAGIPFAEMSQIVGKQVNRPVAKDETLMPNMVKTYRA-AK spotted wolffish-S2 mksailtgllfvllcvdhmssaSQ--SVVATQLIPINTAL TPAMMKGKVVSPAGIPFAEMSQIVGKQVNRPVAKDETLMPNMVKTYRA-AK rock gunnel-S1* mnsailtgflfvllcvdnmtsaGS--SVVASQLIPMNTAL TPAMMKGVVVSPAGIPFAEMSRIVGKQVNQIVAKDQTLMPSMVKTYQP-AK radiated shanny-S1* mnsailtgflfvllcv-nmssaAQ--SVVATQLIPINTAL TPAMMKGMDVNPSGIPFTEKSTLVGKQVNQPVVKGQTLMRNMVKP ocean pout-S1 mksviltgllfvllcvdhm-taSQ--SVVATQLIPINTAL TPAMMEGKVTNPIGIPFAEMSQIVGKQVNTPVAKGQTLMPNMVKTYVA-GK ocean pout-S2* ------cvdhi-taSQ--SVVATRLIPMNTAL TPAMMEGKVTNPIGIPFAEMSQIVGKQVNRIVAKGQTLMPNMVKTYAA-GK ocean pout-S3 mksviltgllfvllcvdhm-taSQ--SVVATQLIPMNSAL TPV MMEGKVTNPIGIPFAEMSQMVGKQVNRPVAKGQTIMPNMVKTYAA-GK ocean pout-S4* ------cvdhm-taSQ--SVVATQLIPMNTAL TPV MMEGKVTNPIGIPFAEMSQIVGKQVNTPVAKGQTIMPNMVKTYAA-GK viviparous eelpout-S1 mkcviltgllfvllcvdhmssaGE--SVVATQLIPINTAL TPAMMAGKVTNPSGIPFAEMSQIVGKQVNTPVAKGQTLMPDMVKTYVP-AK notched-fin eelpout-S1 mksviltgllfvllcvdhmssaGE--SVVATQLIPINTAL TPAMMEGKVTNPSGIPFAEMSQIVGKQVNTPVAKGQTLMPGMVKTYVP-AK viviparous eelpout-S2 ------GE--SVVATQLIPMNTAL TLA MIEGKVTNPSGIPFAEKSQIVGKQVNTPVAKGQTLMPDMVKTYVP-AK viviparous eelpout-S3† mkcviltgllfvllcvdhmssaGE--SVVATQLIPMNTAL TPAMMAGKVTNPSGIPFAEMLQIVGKQVNVIVAKGQTIMPNMVKTYAA-GK viviparous eelpout-S4† mkcviltgllfvllcvdhmssaGE--SVVATQLIPMNTAL TLA MIEGKVTNPSGIPFAEKSQIVGKQVNVIVAKGQTIMPIMVKTYVP-AK viviparous eelpout-S5† mksviltgllfvllcvdhmssaGQ--SVVATQLIPMNTAL TPAMMEGKVTNPSGIPFAEMSQIVGKQVNVIVPKGHTIMPIMVKTYA--GK notched-fin eelpout-S2 mksviltgllfvllcvdhmssaGE--SVVATQLIPINTAL TPAMMEGKVTNPSGIPFAEMSQIVGKQVNVIVAKGQTLMPDMVKTYA--GK viviparous-eelpout-S6† mkcviftgllfvllcvdhmssaGE--SVVATQLIPMNTAL TPAMMAGKVTNPSGIPFAEMSQIVGKQVNVIVAKGQTLMPDMVKTYA--GK viviparous-eelpout-S7† mksvivtgllfvllcvdhm-taGQ--SVVATQLIPMNTAL TLV MMEGKVTNPIGIPFAERDQIVGKQVNVIVAKGQTIMPGMVKTYA--GK viviparous-eelpout-S8† ------hmssaGE--SVVATQLIPMNTAL TLV MMEGKVTNPIGIPFGERDQIVGKQVNVIVAKGQTIMPDMVKTYA--GK viviparous-eelpout-S9† ----iltgllfvllcvdhmssaGE--SVVATQLIPMNTAL TLV MMEGKVTNPSGIPFAEKSQIVGKQVNVIVAKGQTIMPDMVKTYA--GK viviparous-eelpout-S10† mksviltgllfvllcvdhmssaGE--SVVATQLIPMNTAL TPAMMAGKVTNPSGIPFVEASQIVGKQVNVIVPKGQTIMPDMVKTYA--GK notched-fin eelpout-S3 mksviltgllfvllcvdhmssaGQ--SVVATQLIPMNTAL TLA MMEGKVTNPSGIPFVEASQIVGKQVNVIVAKGQTIMPIMVKTYA--GK notched-fin eelpout-S4 ------GQ--SVVATQLIPMNTAL TPAMMEGKVTNPSGIPFVEASQIVGKQVNVIVAKGQTLMPDMVKTYA--GK *:..:.**:.:***** :: :* * ** ***:*:** * * * *** : ::. **: * . *: ***