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Home , Calponin 1, CHEK1, Chromosome 9, DPM3, GJA4, IL1A

Supplemental figure 1. LSECs suppress T production and proliferation via PD-1/PD-L1 interaction. (A) LSECs were stained by either isotype control or anti-CD146, anti-NK1.1, anti-DX5, anti-CD11b, anti-CD11c, and anti-F4/80 after magnetic microbead purification. The purity of the cell fractions was then measured by flow cytometry. (B) Unstimulated or anti-CD3 (1 μg/ml) and anti-CD28 (1 μg/ml) stimulated splenocytes from C57BL/6 mice were cultured with or without LSECs at a ratio of 1:2 (LSECs:splenocytes). cytokine production and proliferation were measured after 48 h. Anti-CD3/CD28 stimulated splenocytes only were used as responder controls (RC). Unstimulated splenocytes alone were used as negative control (NC). Unstimulated splenocytes cocultured with LSECs were used as unstimulated control (UC). (C) LSECs were cocultured with polyclonal-stimulated splenocytes in contact or transwell (LSECs and splenocytes separated by a semipermeable membrane) 96-well plates. T cell cytokine production and proliferation were measured after 48 h. (D) LSECs were incubated with 10 μg/ml isotype control antibodies (iso Ab) or anti-PD-L1 (αPD-L1) for 1 h. Polyclonal stimulated splenocytes were cultured with antibodies treated LSECs. T cell cytokine production and proliferation were measured after 48 h. Data shown are the mean ± SD of one out of 3 representative experiments. *p < 0.05; **p < 0.01.

Supplemental figure 2. TLR1/2 signaling does neither increase the costimulatory molecules expression nor the PD-L1 expression on LSECs. LSECs were stimulated in vitro by 10 μg/ml P3C(TLR1/2), 10 μg/ml poly(I:C)(TLR3), 10 μg/ml LPS(TLR4), or 50 ng/ml IFN-γ for either 24 h (A) or 6 h (B). (A) The changes of CD80, CD86, and PD-L1

1

expression on cells were then determined by flow cytometry. (B) The changes of CD80,

CD86, and PD-L1 expression at the RNA level were determined by real-time RT-PCR.

Results are representative of two independent experiments. (C) C57BL/6 mice were intraperitoneally injected with PBS or 100 μg P3C. LSECs from those animals were obtained 24 h later. The expression of CD80, CD86, and PD-L1 was determined by flow cytometry. (D) C57BL/6 mice were intraperitoneally injected with PBS or 100 μg P3C.

Splenic DCs from those animals were obtained 24 h later. The expression of CD80 and

CD86 was determined by flow cytometry.

Supplemental figure 3. TLR1/2 stimulated LSECs do not inhibit activation induced

CD8 T cell apoptosis. (A) Unstimulated or anti-CD3 (1 μg/ml) and anti-CD28 (1 μg/ml) stimulated splenocytes from C57BL/6 mice were cultured with or without LSECs at a ratio of 1:2 (LSECs:splenocytes). CD 8 T cell apoptosis and death were analyzed by using Annexin V and 7-AAD staining after 48 h. Anti-CD3/CD28 stimulated splenocytes only were used as responder controls (RC). Unstimulated splenocytes alone were used as negative control (NC). (B) P3C pretreated or untreated LSECs pulsed with 2 µg/ml FV peptide (FV GagL CTL epitope aa 85-93). Naïve FV-TCR TCR transgenic CD8+ T cells were cocultured with those LSECs or cultured alone (NC). As positive control (PC), the T cells were cocultured with bone marrow-derived FV peptide-pulsed DCs. CD 8 T cell apoptosis and death were analyzed by using Annexin V and 7-AAD staining after 72 h.

Annexin V positive and 7-AAD negative cells are considered as early apoptosis cells and

Annexin V and 7-AAD double positive cells are considered as end stage apoptosis and death cells.

2

Supplemental table 1. expression difference analysis in P3C stimulated LSECs. In

the table, the fold changes (log2 Ratio) of , which were significantly regulated in

P3C stimulated LSECs (compared to untreated LSEC), are shown. Significantly regulated

genes were selected using the following criteria: fold change>2 (Log2 Ratio≥ ±1), false discovery rate (FDR)<0.001.

3 A

n.s. n.s. B * 60000 ** 150000 C 60000 150000 * ** 40000 100000 40000 100000 pg/ml pg/ml   20000 50000 20000 50000 IFN- IFN- Proliferation cpm Proliferation cpm 0 0 0 0 NC UC RC LSEC NC UC RC LSEC RC Contact Transwell RC Contact Transwell ¢CD3/¢CD28 - - + + ¢CD3/¢CD28 - - + + LSEC - + - + LSEC - + - + n.s. n.s. 5000 * 800 * * 5000 * 800 4000 4000 600 600 3000 3000 pg/ml pg/ml

 400

 400 2000 2000 IL-2 pg/ml IL-2 pg/ml TNF- 200 TNF- 200 1000 1000

0 0 0 0 NC UC RC LSEC NC UC RC LSEC RC Contact Transwell RC Contact Transwell ¢CD3/¢CD28 - - + + ¢CD3/¢CD28 - - + + LSEC - + - + LSEC - ++-

n.s. n.s. D 50000 150000 ** ** 40000 100000 30000 pg/ml 20000  50000 IFN- 10000 Proliferation cpm Proliferation 0 0 RC Iso Ab PD-L1 RC Iso Ab PD-L1

n.s. n.s. ** 8000 1000 * 800 6000 600 pg/ml

4000  400 IL-2 pg/ml IL-2 2000 TNF- 200

0 0 RC Iso Ab PD-L1 RC Iso Ab PD-L1

Supplementary Table 1: difference analysis in P3C stimulated LSECs. In the table, the fold changes (log2 Ratio) of genes,which were significantly regulated in P3C stimulated LSECs (compared to untreated LSEC) are shown. Significantly regulated genes were selected using the following criteria: fold change>2 (Log2 Ratioı ±1), false discovery rate (FDR)<0.001.

Symbols Gene names log2 Ratio Acpp acid , prostate, isoform CRA_b 9.44 Gm6578 39S ribosomal L32, mitochondrial 9.40 Il1b -1 beta precursor 6.61 Pls1 plastin-1 5.86 Depdc1a DEP domain-containing protein 1A isoform 3 5.16 Taz , isoform CRA_c 4.52 Chgb secretogranin-1 precursor 4.20 Nit1 nitrilase homolog 1 isoform 1 4.17 Ccl20 C-C motif 20 isoform 1 4.02 Fpr1 fMet-Leu-Phe receptor 3.81 Mmp13 3 preproprotein 3.57 Rgs4 regulator of G-protein signaling 4 3.46 Il1a interleukin 1 alpha, isoform CRA_a 3.21 Nox4 NADPH oxidase 4 3.02 Ccl3 C-C motif chemokine 3 2.88 Kcnh1 potassium voltage-gated channel subfamily H member 1 isoform 2 2.83 Clec4a1 C-type lectin domain family 4, member a1 2.81 Cxcl5 C-X-C motif chemokine 5 precursor 2.78 Steap4 metalloreductase STEAP4 2.76 Il18r1 interleukin-18 receptor 1 isoform a 2.74 Cenpe -associated protein E 2.69 Fpr2 formyl peptide receptor 2 2.69 Serf1 small EDRK-rich factor 1 2.64 Gm6377 novel protein 2.58 Cenph centromere protein H 2.58 Irg1 immune-responsive gene 1 2.58 Kif18a kinesin-like protein KIF18A 2.58 Cnr1 cannabinoid receptor 1 2.56 9930023K05Rik RIKEN cDNA 9930023K05, isoform CRA_c 2.56 Pilra paired immunoglobulin-like type 2 receptor alpha precursor 2.55 Nusap1 nucleolar and spindle-associated protein 1 isoform a 2.53 4930547N16Rik mCG16776, isoform CRA_b 2.52 Esco2 N-acetyltransferase ESCO2 2.48 Hjurp Holliday junction recognition protein 2.44 Ccl9 C-C motif chemokine 9 2.40 Cxcl2 C-X-C motif chemokine 2 precursor 2.38 Fam64a family with sequence similarity 64, member A 2.38 Prune2 protein prune homolog 2 2.34 Ccnb2 G2/mitotic-specific cyclin-B2 2.32 Aspm abnormal spindle-like microcephaly-associated protein homolog 2.32 Prelid2 PRELI domain-containing protein 2 2.29 Sncaip synuclein, alpha interacting protein (synphilin), isoform CRA_b 2.28 Tmem71 transmembrane protein 71 2.28 Mmp9 matrix metalloproteinase-9 precursor 2.28 Kif15 kinesin-like protein KIF15 2.28 Ccl6 C-C motif chemokine 6 precursor 2.24 Cxcl3 C-X-C motif chemokine 3 precursor 2.20 1-Mar E3 ubiquitin-protein MARCH1 isoform 3 2.20 Il33 interleukin-33 precursor 2.20 Fam36a Protein FAM36A 2.20 Scd2 acyl-CoA desaturase 2 2.19 Snhg3 mCG118858 2.17 Kif20b kinesin-like protein KIF20B 2.16 Pkd2l2 polycystic disease 2-like 2 protein 2.16 Psg22 mCG3735 2.16 Mis18bp1 expressed sequence C79407 2.15 Cldn2 claudin-2 2.15 8430408G22Rik protein DEPP 2.15 Hells -specific helicase 2.14 Casc5 protein CASC5 2.13 Myh15 -15 2.12 Kctd12 BTB/POZ domain-containing protein KCTD12 2.10 Csf3r colony-stimulating factor receptor precursor 2.08 2810417H13Rik mCG131663, isoform CRA_b 2.06 Cybb cytochrome b-245 heavy chain 2.06 Clec1a C-type lectin domain family 1 member A 2.06 Ect2 mKIAA4037 protein 2.04 C330027C09Rik RIKEN cDNA C330027C09, isoform CRA_a 2.03 Sgol1 shugoshin-like 1 2.02 C030044B11Rik mCG1043891 2.01 Rassf4 Ras association (RalGDS/AF-6) domain family member 4 2.00 St8sia4 ST8 alpha-N-acetyl-neuraminide alpha-2,8- 4 2.00 Efha2 EF-hand domain-containing family member A2 1.99 Prr11 proline-rich protein 11 1.98 Fam83b Protein FAM83B 1.97 Clec4n C-type lectin domain family 6 member A isoform 1 1.97 Enpp2 ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform 1 1.97 Trp53i11 Trp53 inducible protein 11, isoform CRA_d 1.94 Prrg1 proline rich Gla (G-carboxyglutamic acid) 1 1.94 5830433M19Rik likely ortholog of H. sapiens 9 open reading frame 82 (C9orf82) 1.93 Cdca2 cell division cycle-associated protein 2 1.92 Cd14 differentiation antigen CD14 precursor 1.92 Col4a3 collagen alpha-3(IV) chain precursor 1.90 Gpr84 G-protein coupled receptor 84 1.90 Ccl4 C-C motif chemokine 4 1.89 Sorcs1 VPS10 domain-containing receptor SorCS1 1.89 Cenpf centromere protein F 1.89 Fam83d mCG15735 1.88 Bub1 budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae), isoform CRA_a 1.87 Kif14 kinesin-like protein KIF14 1.86 Fam132b Protein FAM132B 1.85 Muc1 mucin-1 1.85 Plch1 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase eta-1 isoform 2 1.85 Ccdc15 coiled-coil domain-containing protein 15 1.84 Ube2c ubiquitin-conjugating E2 C 1.84 Cxcr4 C-X-C chemokine receptor type 4 1.82 Igfbp3 -like -binding protein 3 1.82 Clec7a C-type lectin domain family 7 member A 1.81 Lcp2 lymphocyte cytosolic protein 2 1.80 Hmmr hyaluronan mediated motility receptor 1.79 Kif11 kinesin family member 11, isoform CRA_b 1.79 Spic Spi-C 1.79 Lrch2 leucine-rich repeats and homology (CH) domain containing 2 1.78 Gulp1 PTB domain-containing engulfment adapter protein 1 1.78 Fam199x cDNA sequence BC031748 1.78 Tpx2 TPX2, microtubule-associated protein homolog (Xenopus laevis), isoform CRA_b 1.77 Ms4a6d membrane-spanning 4-domains subfamily A member 6D 1.76 Ccl7 C-C motif chemokine 7 1.75 Kif23 kinesin family member 23 1.74 Ptpn4 tyrosine-protein phosphatase non-receptor type 4 1.73 Abcc9 ATP-binding cassette sub-family C member 9 isoform a 1.72 Sorl1 sortilin-related receptor 1.72 Pbk -activated killer T-cell-originated protein 1.71 Mastl microtubule-associated serine/threonine-protein kinase-like 1.70 Ehf ets homologous factor, isoform CRA_d 1.70 Mybl1 myeloblastosis oncogene-like 1, isoform CRA_a 1.69 Gen1 flap endonuclease GEN homolog 1 1.69 Dock11 dedicator of cytokinesis protein 11 1.68 Fam169a Fam169a protein 1.67 Cep55 centrosomal protein 55, isoform CRA_d 1.67 Col4a4 collagen alpha-4(IV) chain precursor 1.66 Sass6 spindle assembly abnormal protein 6 homolog 1.65 Ndc80 kinetochore protein NDC80 homolog 1.65 Eya4 eyes absent homolog 4 1.64 Kcne3 potassium voltage-gated channel subfamily E member 3 1.64 C1galt1 glycoprotein-N-acetylgalactosamine 3-beta- 1 1.64 Vash1 vasohibin-1 1.64 Idi1 isopentenyl-diphosphate Delta- 1 1.63 Plek pleckstrin 1.62 Insig1 insulin-induced gene 1 protein 1.62 Elovl6 elongation of very long chain fatty acids protein 6 1.62 Cp ceruloplasmin, isoform CRA_c 1.61 Lyve1 lymphatic vessel endothelial hyaluronic acid receptor 1 precursor 1.61 Marco receptor MARCO 1.61 Ccna2 cyclin-A2 1.60 Mki67 antigen KI-67 1.60 Siglece sialic acid binding Ig-like lectin E 1.60 Prpf40a pre-mRNA-processing factor 40 homolog A 1.59 Ncapg non-SMC condensin I complex, subunit G 1.58 Chm rab geranylgeranyltransferase component A 1 1.58 Slx1b GIY-YIG domain containing 2, isoform CRA_d 1.57 Lin9 protein lin-9 homolog isoform 1 1.57 Anln anillin, binding protein (scraps homolog, Drosophila), isoform CRA_b 1.57 Utp14b U3 small nucleolar RNA-associated protein 14 homolog B 1.56 Acin1 apoptotic condensation inducer in the nucleus isoform 4 1.56 Kif20a kinesin family member 20A, isoform CRA_b 1.55 Prss22 brain-specific serine 4 1.55 Dram1 DNA damage-regulated autophagy modulator protein 1 1.55 Emr1 EGF-like module containing, mucin-like, hormone receptor-like sequence 1, isoform CRA_c 1.55 Slc7a11 cystine/glutamate transporter 1.54 Ctsc dipeptidyl peptidase 1 preproprotein 1.54 Gsta3 glutathione S- A3 1.54 Ttk Ttk protein kinase, isoform CRA_c 1.54 Zdhhc2 palmitoyltransferase ZDHHC2 1.54 Mfap3l microfibrillar-associated protein 3-like, isoform CRA_b 1.54 Zcchc10 zinc finger, CCHC domain containing 10, isoform CRA_b 1.54 Ccbe1 collagen and calcium binding EGF domains 1, isoform CRA_e 1.53 Hsd17b7 3-keto- reductase 1.53 Rp2h protein XRP2 1.52 Pcmtd1 rCG30411, isoform CRA_b [Rattus norvegicus] 1.51 Nbeal1 neurobeachin like 1 1.51 Msr1 macrophage scavenger receptor types I and II isoform a 1.51 Cmah cytidine monophospho-N-acetylneuraminic acid hydroxylase, isoform CRA_c 1.50 Trim59 tripartite motif-containing protein 59 1.50 Itga2 alpha-2 precursor 1.49 Lpar4 lysophosphatidic acid receptor 4 [Rattus norvegicus] 1.49 Smc2 structural maintenance of protein 2 1.49 Il1rn interleukin-1 receptor antagonist 2 1.48 Atad2 ATPase family AAA domain-containing protein 2 1.48 Cav2 caveolin-2 1.48 Acsl3 long-chain-fatty-acid--CoA ligase 3 isoform a 1.47 Cep290 centrosomal protein of 290 kDa 1.47 Ccdc88a girdin 1.46 Clec4e C-type lectin domain family 4 member E 1.46 Scd1 acyl-CoA desaturase 1 1.45 F8 coagulation factor VIII isoform 3 1.45 Pak3 serine/threonine-protein kinase PAK 3 isoform C 1.45 Vma21 vacuolar ATPase assembly integral membrane protein VMA21 1.45 Aurka serine/threonine-protein kinase 6 1.43 Pla2g7 -activating factor acetylhydrolase 1.43 Slc4a7 sodium bicarbonate cotransporter 3 1.43 Fam46a mCG11933 1.43 Ckap2l associated protein 2-like, isoform CRA_a 1.42 Rnf125 E3 ubiquitin-protein ligase RNF125 1.42 Yes1 Yamaguchi sarcoma viral (v-yes) oncogene homolog 1, isoform CRA_b 1.42 Wisp1 WNT1 inducible signaling pathway protein 1, isoform CRA_d 1.41 Rnf122 ring finger protein 122, isoform CRA_b 1.41 Tspan2 tetraspanin-2 1.41 D19Ertd737e DNA segment, Chr 19, ERATO Doi 737, expressed, isoform CRA_d 1.41 Galnt3 polypeptide N-acetylgalactosaminyltransferase 3 1.41 Tmtc3 transmembrane and TPR repeat-containing protein 3 isoform 2 1.40 Marcks myristoylated alanine-rich C-kinase [Bos taurus] 1.40 Stk3 serine/threonine-protein kinase 3 1.40 Anxa13 annexin A13 1.39 Adamts4 A disintegrin and metalloproteinase with thrombospondin motifs 4 1.39 Abca5 ATP-binding cassette sub-family A member 5 1.39 Prc1 protein regulator of cytokinesis 1, isoform CRA_d 1.39 Fst follistatin 1.39 Treml4 triggering receptor expressed on myeloid cells-like 4, isoform CRA_c 1.38 Zfp800 zinc finger protein 800 1.38 Ccnc cyclin C, isoform CRA_d 1.38 Top2a DNA topoisomerase 2-alpha 1.38 Myo1b myosin-Ib isoform 2 1.38 Hmgn5 GARP45 1.38 Rb1 retinoblastoma-associated protein 1.37 Atp11c probable phospholipid-transporting ATPase 11C isoform a 1.37 Lcorl -dependent nuclear receptor corepressor-like protein isoform 1 1.37 Kif4 chromosome-associated kinesin KIF4 1.36 Rad51ap1 RAD51-associated protein 1 1.36 AA467197 normal mucosa of esophagus-specific gene 1 protein 1.36 Gtpbp10 GTP-binding protein 10 1.36 Tmx3 mKIAA1830 protein 1.35 Birc5 baculoviral IAP repeat-containing protein 5 isoform 1 1.35 Aldh1a2 retinal dehydrogenase 2 1.35 Usp45 ubiquitin carboxyl-terminal 45 1.34 Iqgap3 IQ motif containing GTPase activating protein 3 1.34 7-Mar E3 ubiquitin-protein ligase MARCH7 1.34 Dnajb9 DnaJ (Hsp40) homolog, subfamily B, member 9 1.34 Chek1 serine/threonine-protein kinase Chk1 1.32 Smc4 structural maintenance of chromosomes protein 4 1.32 Hif1a hypoxia inducible factor 1, alpha subunit, isoform CRA_b 1.32 Ccdc34 coiled-coil domain-containing protein 34 1.31 Spp1 secreted phosphoprotein 1, isoform CRA_d 1.31 Gmnn geminin 1.31 Prkcb beta type 1.31 Prkaa2 5'-AMP-activated protein kinase catalytic subunit alpha-2 1.30 Spc25 spindle pole body component 25 homolog (S. cerevisiae), isoform CRA_b 1.30 Fam117a RIKEN cDNA 5730593F17 1.30 Rab11fip2 Rab11-family interacting protein 2 isoform 1 1.30 Cdc20 cell division cycle protein 20 homolog 1.30 Lysmd3 lysM and putative peptidoglycan-binding domain-containing protein 3 1.29 Fmr1 fragile X mental retardation protein 1 homolog 1.29 Manea glycoprotein endo-alpha-1,2-mannosidase 1.29 Lrrcc1 mKIAA1764 protein 1.29 Vkorc1l1 vitamin K epoxide reductase complex subunit 1-like protein 1 isoform 2 1.29 Zfp518a zinc finger protein 518A 1.28 Gm11428 mCG55370 1.28 Fyb FYN-binding protein 1.28 Fsd1l FSD1-like protein isoform 1 1.28 F630043A04Rik RIKEN cDNA F630043A04, isoform CRA_b 1.28 Fam101b family with sequence similarity 101, member B 1.28 Mzt1 mCG1042562 1.27 Homer1 homer protein homolog 1 isoform L 1.26 Gng2 mCG144516 1.26 Gpr126 G-protein coupled receptor 126 precursor 1.26 Psd3 PH and SEC7 domain-containing protein 3 isoform 2 1.26 Vamp7 vesicle-associated membrane protein 7 1.26 Nid2 nidogen-2 precursor 1.26 Cpeb4 cytoplasmic polyadenylation element-binding protein 4 1.26 Txlng gamma-taxilin 1.26 Snrnp27 U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein 1.25 Tmed5 Transmembrane emp24 protein transport domain containing 5 1.25 Acsl4 acyl-CoA synthetase long-chain family member 4, isoform CRA_b 1.25 Amot Angiomotin 1.25 Dcdc2a mKIAA1154 protein 1.25 Upf3b regulator of nonsense transcripts 3B 1.24 Plcb4 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-4 1.24 Plod2 procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 isoform 1 1.24 Hs6st2 Heparan-sulfate 6-O-sulfotransferase 2 1.24 Pcdhb16 protocadherin beta 16 1.24 Mme membrane metallo endopeptidase, isoform CRA_a 1.24 Apob apolipoprotein B precursor 1.24 Pde3b cGMP-inhibited 3',5'-cyclic phosphodiesterase B 1.24 Pik3c2a phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha 1.23 Jhdm1d lysine-specific demethylase 7 1.22 Slc12a2 solute carrier family 12 member 2 1.22 Uevld ubiquitin-conjugating enzyme E2 variant 3 1.22 C5ar1 C5a anaphylatoxin chemotactic receptor 1.22 Filip1 -A-interacting protein 1 1.22 Itgam integrin alpha-M isoform 2 precursor 1.22 Suz12 suppressor of zeste 12 homolog (Drosophila), isoform CRA_c 1.22 Cacybp calcyclin binding protein 1.21 Naa50 N-acetyltransferase 13, isoform CRA_c 1.21 A630089N07Rik PREDICTED: putative transposase element L1Md-A101/L1Md-A102/L1Md-A2-like 1.21 Pcmtd2 mKIAA3003 protein 1.21 Cyp26b1 cytochrome P450, family 26, subfamily b, polypeptide 1, isoform CRA_b 1.20 Chml rab proteins geranylgeranyltransferase component A 2 1.20 Scoc short coiled-coil protein isoform a 1.20 Fcgr3 low affinity immunoglobulin gamma Fc region receptor III 1.20 Tnf 1.20 A230046K03Rik WASH complex subunit 7 1.20 Eea1 early endosome antigen 1 1.20 Rrm2 ribonucleotide reductase M2 1.20 Sgol2 shugoshin-like 2 isoform 2 1.20 D730040F13Rik likely ortholog of H. sapiens open reading frame 5 (C9orf5) 1.20 Styx serine/threonine/tyrosine-interacting protein 1.19 Brca2 breast cancer 2 1.18 Atrx transcriptional regulator ATRX 1.18 Tceal8 transcription elongation factor A protein-like 8 1.18 Rock2 rho-associated protein kinase 2 1.18 Dtx4 protein deltex-4 1.18 Zfp36l1 zinc finger protein 36, C3H1 type-like 1 1.17 Cd44 CD44 antigen isoform a precursor 1.17 Cdk1 cyclin-dependent kinase 1 1.17 Adamts5 A disintegrin and metalloproteinase with thrombospondin motifs 5 precursor 1.17 Fam49a DNA segment, Chr 12, ERATO Doi 553, expressed, isoform CRA_b 1.17 Kif2c kinesin-like protein KIF2C 1.17 Il1rap interleukin-1 receptor accessory protein isoform d precursor 1.17 Olfml2b olfactomedin-like protein 2B precursor 1.16 Ammecr1 PREDICTED: AMME syndrome candidate gene 1 protein homolog 1.16 Rbm34 RNA-binding protein 34 1.16 Dbf4 protein DBF4 homolog A isoform 1 1.16 Col4a5 procollagen type IV alpha 5 1.15 Sc4mol C-4 methylsterol oxidase 1.15 Dzip3 E3 ubiquitin-protein ligase DZIP3 isoform 1 1.15 Cul4b cullin 4B 1.15 Psip1 PC4 and SFRS1-interacting protein 1.15 Espl1 extra spindle poles-like 1 (S. cerevisiae), isoform CRA_b 1.15 Ube2g1 ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans), isoform CRA_c 1.15 Fnip1 folliculin-interacting protein 1 1.15 Osbpl8 oxysterol-binding protein-like protein 8 isoform a 1.14 Tbca tubulin-specific chaperone A 1.14 Itgav integrin alpha-V precursor 1.14 Klhl4 kelch-like 4 (Drosophila) 1.14 Dlgap5 discs, large homolog 7 (Drosophila), isoform CRA_a 1.13 Xrn1 5'-3' exoribonuclease 1 1.13 Pou2f1 POU domain, class 2, transcription factor 1 isoform B 1.13 Rif1 -associated protein RIF1 1.13 Sp4 transcription factor Sp4 isoform 2 1.13 Man2a1 mannosidase 2, alpha 1 1.13 Arhgap18 Rho GTPase activating protein 18 1.13 Rg9mtd2 RNA (guanine-9-)-methyltransferase domain-containing protein 2 1.13 Atp7a ATPase, Cu++ transporting, alpha polypeptide, isoform CRA_b 1.13 Tnfsf10 tumor necrosis factor ligand superfamily member 10 1.13 Mad2l1 mCG125314, isoform CRA_b 1.12 Zbtb41 zinc finger and BTB domain-containing protein 41 1.12 A630007B06Rik oocyte-testis gene 1 1.12 Taf1d TATA box-binding protein-associated factor RNA polymerase I subunit D isoform 2 1.12 Calcrl calcitonin gene-related peptide type 1 receptor precursor 1.12 Pter phosphotriesterase related, isoform CRA_a 1.11 Fam38b piezo2 1.11 Mob1b mps one binder kinase activator-like 1A 1.11 Mndal myeloid cell nuclear differentiation antigen-like protein 1.11 Rb1cc1 RB1-inducible coiled-coil protein 1 1.11 4933421E11Rik receptor-interacting factor 1 isoform 1 1.10 Fam63b RIKEN cDNA B230380D07, isoform CRA_d 1.10 Zfp644 zinc finger protein 644 1.10 Acer3 alkaline ceramidase 3 1.10 Olr1 oxidized low density lipoprotein (lectin-like) receptor 1 1.10 Map3k2 -activated protein kinase kinase kinase 2 1.10 Esco1 N-acetyltransferase ESCO1 1.10 Zbed6 zinc finger BED domain-containing protein 6 1.10 Hook3 protein Hook homolog 3 1.10 Cldn1 claudin-1 1.10 Ankrd32 ankyrin repeat domain-containing protein 32 1.09 Sarnp SAP domain-containing ribonucleoprotein 1.09 Steap2 metalloreductase STEAP2 1.09 Hmgb2 high mobility group protein B2 1.09 Dhx36 probable ATP-dependent RNA helicase DHX36 1.09 Ccdc99 protein Spindly 1.09 Casp3 caspase-3 1.09 2700089E24Rik mCG1039243, isoform CRA_c 1.09 Lin7c protein lin-7 homolog C 1.09 Scai protein SCAI 1.09 Ell2 RNA polymerase II elongation factor ELL2 1.09 Arhgap11a mKIAA0013 protein 1.08 Gm3414 Putative uncharacterized protein 1.08 Fktn 1.08 Slfn9 schlafen 9 1.08 3110003A17Rik 3110003A17Rik protein 1.08 Stk17b serine/threonine kinase 17b (apoptosis-inducing), isoform CRA_b 1.07 Tab3 mKIAA4135 protein 1.07 Tanc2 protein TANC2 1.07 Ube2w mCG13474 1.07 Abca1 ATP-binding cassette sub-family A member 1 1.07 1810029B16Rik UPF0534 protein C4 1.07 Pdcd10 programmed cell death protein 10 1.07 Zfp91 E3 ubiquitin-protein ligase ZFP91 1.06 Ggps1 geranylgeranyl pyrophosphate synthase 1.06 Polr3g DNA-directed RNA polymerase III subunit RPC7 1.06 Ptprc protein tyrosine phosphatase, receptor type, C, isoform CRA_b 1.06 7-Sep Septin-7 1.06 Serpinb9 serine (or cysteine) proteinase inhibitor, clade B, member 9 1.06 Itga8 integrin alpha-8 precursor 1.06 Trpm7 transient receptor potential cation channel subfamily M member 7 isoform 2 1.05 Trip11 thyroid hormone receptor interactor 11 1.05 Fignl1 fidgetin-like 1, isoform CRA_b 1.05 Exo1 exonuclease 1 1.04 Dennd1b DENN domain-containing protein 1B isoform 1 1.04 Hmgcs1 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1, isoform CRA_b 1.04 Fam76b Protein FAM76B 1.04 Zbtb10 zinc finger and BTB domain containing 10 1.04 Dr1 hypothetical protein PANDA_006355 1.04 Naa16 N-alpha-acetyltransferase 16, NatA auxiliary subunit 1.04 Lnpep leucyl-cystinyl aminopeptidase 1.04 Mier3 mesoderm induction early response protein 3 1.03 Cenpc1 centromere protein C 1 1.03 Arhgap5 rho GTPase-activating protein 5 1.03 Ncoa7 nuclear receptor coactivator 7 isoform 2 1.03 Cd164 sialomucin core protein 24 1.03 Malat1 mCG1042565, isoform CRA_a 1.03 Pmp22 Peripheral myelin protein 22 1.03 Ugcg ceramide 1.03 Sc5d lathosterol oxidase 1.02 Tfb2m dimethyladenosine transferase 2, mitochondrial 1.02 Ncoa6 nuclear receptor coactivator 6 1.02 Cxcl1 Cxcl1 protein 1.02 Kcnj2 inward rectifier 2 1.02 Prex2 phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein isoform 1 1.02 Sh3kbp1 SH3 domain-containing kinase-binding protein 1 isoform 2 1.02 Prom1 prominin-1 isoform s8 1.01 Klf6 Krueppel-like factor 6 1.01 Gda mKIAA1258 protein 1.01 Phf17 mKIAA1807 protein 1.01 Blm homolog isoform 1 1.01 Arid4b AT-rich interactive domain-containing protein 4B isoform 1 1.01 Cd2ap CD2-associated protein 1.01 E2f7 transcription factor E2F7 1.01 Rnpc3 RNA-binding protein 40 1.00 Mgll monoglyceride lipase isoform d 1.00 Atad5 ATPase family AAA domain-containing protein 5 1.00 Prrg4 transmembrane gamma-carboxyglutamic acid protein 4 precursor 1.00 4921524J17Rik UPF0547 protein C16orf87 homolog 1.00 Apobec3 DNA dC->dU-editing enzyme APOBEC-3 isoform 1 1.00 Pdk2 kinase, isoenzyme 2 precursor -1.00 Plxna4 plexin-A4 precursor -1.00 Abi3 phosphoethanolamine/phosphocholine phosphatase [Rattus norvegicus] -1.00 Pnkp polynucleotide kinase 3'- phosphatase, isoform CRA_d -1.01 Zfpm1 zinc finger protein ZFPM1 -1.01 Pdrg1 and DNA damage-regulated protein 1 -1.02 C1qb complement C1q subcomponent subunit B precursor -1.02 Fbn1 fibrillin-1 -1.02 Xdh xanthine dehydrogenase, isoform CRA_a -1.02 Rrp9 U3 small nucleolar RNA-interacting protein 2 -1.02 Cpt2 carnitine O-palmitoyltransferase 2, mitochondrial precursor -1.03 Shroom1 protein Shroom1 -1.04 Prmt2 protein arginine N-methyltransferase 2 -1.04 Polr2i mCG22814 -1.04 Stk40 serine/threonine kinase 40, isoform CRA_b -1.04 Bst2 bone marrow stromal cell antigen 2, isoform CRA_b -1.05 Ccs copper chaperone for superoxide dismutase -1.05 Fbxw4 F-box/WD repeat-containing protein 4 -1.05 Dmpk myotonin-protein kinase isoform 2 -1.06 Fam203a brain protein 16 -1.06 Cd5l CD5 antigen-like precursor -1.06 Telo2 RIKEN cDNA 1200003M09, isoform CRA_c -1.06 Xaf1 XIAP-associated factor 1 -1.06 Slc25a19 mitochondrial thiamine pyrophosphate carrier -1.07 0910001L09Rik mCG7441, isoform CRA_a -1.07 Cebpa CCAAT/enhancer-binding protein alpha -1.07 Mib2 E3 ubiquitin-protein ligase MIB2 -1.08 Eng isoform 2 -1.08 Nenf neudesin precursor -1.08 Tmem204 transmembrane protein 204 -1.08 Eppk1 epiplakin -1.09 Calhm2 RIKEN cDNA 2810048G17, isoform CRA_a -1.09 Rspo3 R-spondin-3 precursor -1.09 H2-D1 H-2 class I histocompatibility antigen, L-D alpha chain precursor -1.10 Atf5 cyclic AMP-dependent transcription factor ATF-5 -1.10 Rapgef3 rap guanine nucleotide exchange factor 3 isoform 3 -1.10 Snai1 zinc finger protein SNAI1 -1.10 Fhdc1 mKIAA1727 protein -1.11 Nr1d1 nuclear receptor subfamily 1 group D member 1 -1.11 Ndufs7 NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial precursor -1.12 Tap1 antigen peptide transporter 1 isoform 2 -1.12 Krt8 keratin, type II cytoskeletal 8 -1.13 Hspb1 beta-1 -1.13 Mrps12 28S ribosomal protein S12, mitochondrial precursor -1.14 Mad2l1bp mKIAA0110 protein -1.14 Abcc10 multidrug resistance-associated protein 7 isoform mrp7B -1.14 Rinl RIKEN cDNA 5830482F20, isoform CRA_a -1.15 Twf2 twinfilin-2 -1.15 Id1 helix-loop-helix protein (Id) -1.16 Gata4 transcription factor GATA-4 -1.16 Igsf9 immunoglobulin superfamily, member 9, isoform CRA_a -1.17 Acads short-chain specific acyl-CoA dehydrogenase, mitochondrial precursor -1.17 Alb serum albumin precursor -1.17 Pknox2 homeobox protein PKNOX2 -1.18 Nbl1 neuroblastoma suppressor of tumorigenicity 1 precursor -1.19 Armc6 mCG23104, isoform CRA_b -1.20 Mettl11a alpha N-terminal protein methyltransferase 1A -1.20 Pcyox1l prenylcysteine oxidase-like precursor -1.22 Htra1 serine protease HTRA1 precursor -1.22 Zfp59 zinc finger protein 59 -1.24 Tfeb transcription factor EB, isoform CRA_c -1.25 Hint2 histidine triad nucleotide-binding protein 2, mitochondrial precursor -1.26 Rhod rho-related GTP-binding protein RhoD precursor -1.27 Bmp6 bone morphogenetic protein 6, isoform CRA_a -1.27 Ltbp4 latent-transforming growth factor beta-binding protein 4 isoform b -1.28 Neat1 mCG148129 -1.29 Adcy1 adenylate cyclase type 1 -1.30 Fam171a2 Protein FAM171A2 -1.31 AU021092 UPF0764 protein C16orf89 homolog -1.32 Entpd1 ectonucleoside triphosphate diphosphohydrolase 1, isoform CRA_b -1.35 Ehd3 EH domain-containing protein 3 -1.36 Cspg4 chondroitin sulfate proteoglycan 4 precursor -1.37 Ccdc48 coiled-coil domain-containing protein 48 -1.39 Hs3st1 heparan sulfate glucosamine 3-O-sulfotransferase 1 precursor -1.42 Vwf Von Willebrand factor homolog -1.43 Ceacam1 carcinoembryonic antigen-related cell adhesion molecule 1 isoform 1 -1.44 Psmg4 proteasome assembly chaperone 4 isoform b -1.46 Il18bp interleukin-18-binding protein precursor -1.46 H2-T23 H-2 class I histocompatibility antigen, D-37 alpha chain precursor -1.47 Smad7 MAD homolog 7 (Drosophila), isoform CRA_a -1.47 5430435G22Rik RIKEN cDNA 5430435G22, isoform CRA_a -1.50 Flnc filamin-C -1.50 Smarcd3 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 -1.51 Oas1a 2'-5'-oligoadenylate synthase 1A -1.52 Ace angiotensin-converting enzyme isoform 1 -1.54 Lgals3 Galectin-3 -1.55 Smad6 mothers against decapentaplegic homolog 6 -1.57 E4f1 transcription factor E4F1 -1.57 Adap2 arf-GAP with dual PH domain-containing protein 2 -1.59 Snta1 syntrophin, acidic 1, isoform CRA_a -1.61 Boc brother of CDO precursor -1.62 Krt17 keratin, type I cytoskeletal 17 -1.62 Trem2 TREM2B splice variant -1.63 Id3 DNA-binding protein inhibitor ID-3 -1.67 H2-Q8 H-2 class I histocompatibility antigen, Q8 alpha chain -1.68 Dpm3 dolichol-phosphate subunit 3 -1.71 Efhd1 EF hand domain containing 1, isoform CRA_c -1.71 Bmper BMP-binding endothelial regulator protein precursor -1.71 Gja4 alpha-4 protein -1.71 Pf4 precursor -1.71 Zfp862 zinc finger protein 862-like -1.71 Gata2 endothelial transcription factor GATA-2 -1.72 Zfp775 zinc finger protein 775 -1.74 3000002C10Rik Glyceraldehyde-3-phosphate dehydrogenase -1.75 Cnn1 calponin-1 -1.78 Tgfbi transforming growth factor-beta-induced protein ig-h3 precursor -1.78 Lims2 LIM and senescent cell antigen-like-containing domain protein 2 -1.81 H2-Q6 histocompatibility 2, Q region 6 -1.82 Cd74 H-2 class II histocompatibility antigen gamma chain isoform 2 -1.83 Cd300lb CMRF35-like molecule 7 -1.84 Rasl11b ras-like member 11B -1.84 Rn7sk zinc finger CCCH-type domain containing 3 -1.86 Adamts2 A disintegrin and metalloproteinase with thrombospondin motifs 2 -1.87 H2-Ab1 histocompatibility 2, class II antigen A, beta 1 precursor -1.89 Nudt7 nudix (nucleoside diphosphate linked moiety X)-type motif 7, isoform CRA_b -1.90 Nr4a1 nuclear receptor subfamily 4 group A member 1 -1.96 Pltp phospholipid transfer protein precursor -1.97 Tmem200b transmembrane protein 200B [Homo sapiens] -2.05 Atoh8 protein atonal homolog 8 -2.08 Plac8 placenta-specific gene 8 protein -2.12 Zbp1 Z-DNA-binding protein 1 isoform 2 -2.17 Vsig4 V-set and immunoglobulin domain-containing protein 4 -2.34 H2-Aa histocompatibility 2, class II antigen A, alpha precursor -2.44 Ly6a lymphocyte antigen 6 complex, locus A, isoform CRA_b -2.44 Klhdc8a kelch domain containing 8A, isoform CRA_b -2.56 Igf2 insulin-like growth factor II isoform 1 preproprotein -2.75 Cyp1a1 cytochrome P450 1A1 isoform 1 -2.90 Irf7 regulatory factor 7 -2.97 C4b complement C4-B precursor -3.21 Foxn1 forkhead box protein N1 -3.21 Pdlim3 PDZ and LIM domain protein 3 -3.37 Dio3 type III iodothyronine deiodinase -4.44 Blk B lymphoid kinase -10.34