Human TGFBI / BIGH3 (Fasciclin Domain 4) - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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SA6045X Human TGFBI / BIGH3 (Fasciclin domain 4) - Purified

Alternate names: Beta ig-h3, Kerato-epithelin, RGD-CAP, RGD-containing collagen-associated protein, Transforming growth factor-beta-induced protein ig-h3 Quantity: 0.5 mg Concentration: 1.0 mg/ml Background: BIGH3, also known as TGFBI and Beta ig-h3, is an extracellular matrix protein induced by transforming growth factor(TGF)-beta 1. BIGH3 protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of BIGH3 were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain). Uniprot ID: Q15582 NCBI: NP_000349.1 GeneID: 7045 Species: Human Source: E. coli Format: State: Liquid purified protein Purity: >95% by SDS-PAGE Buffer System: 20 mM Tris, pH 8.0 Description: Recombinant human BIGH3 protein (fourth FAS domain) was expressed in E. coli and purified by using conventional chromatography techniques. AA Sequence: MGTVMDVLKG DNRFSMLVAA IQSAGLTETL NREGVYTVFA PTNEAFRALP PRERSRLLGD AKELANILKY HIGDEILVSG GIGALVRLKS LQGDKLEVSL KNNVVSVNKE PVAEPDIMAT NGVVHVITNV LQPPA Molecular weight: 14 kDa (135 aa, residues 502-636) Storage: Store (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: Billings PC, et al. (2002). J Biol Chem. 277. 28003–28009. Munier FL. et al. (2002). Invest Ophthalmol Vis Sci. 43.949–954. Jung-Eun Kim, et al.(2000) J Biol Chem. 275. 30907–30915.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20130116 1 / 2 SA6045X: Human TGFBI / BIGH3 (Fasciclin domain 4) - Purified

Pictures:

Recombinant human BIGH3 (aa 502-636): 15% SDS PAGE

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20130116 2 / 2