BGI RNA-Seq (Transcriptome) Sequencing

SERVICE OVERVIEW

Service Description Project Workflow Transcriptome sequencing is used to reveal the presence, quantity We care for your samples from the start through and structure of RNA in a biological sample under specific conditions. to the result reporting. Highly experienced Compared to hybridization-based RNA quantification methods such laboratory professionals follow strict quality as microarray analysis, sequencing-based transcriptome detection procedures to ensure the integrity of your can quantify gene expression with low background, high accuracy and results. high levels of reproducibility within a large dynamic range. In addition, transcriptome sequencing does not require an existing genome sequence and can detect mutations/SNPs, splice variants and fusion genes that can not be obtained by microarrays. SAMPLE PREPARATION BGI also provides Whole Transcriptome sequencing, which provides sequencing information for both mRNA and Long non-coding RNA Sample QC (lncRNA). Whole Transcriptome Sequencing can identify novel lncRNAs besides detection and quantification of known lncRNAs. LIBRARY Sequencing Service Specification CONSTRUCTION BGI transcriptome sequencing services are executed with the Illumina HiSeq 4000 sequencing system. Library QC • Multiple choices for mRNA enrichment and rRNA removal kits • Regular and strand-specific transcriptome library SEQUENCING • 100bp and 150bp paired-end sequencing options available • Raw data and bioinformatics analysis are available in Sequencing QC standard file formats • Advanced and custom bioinformatics data analysis • Cloud-based data storage and delivery system RAW DATA OUTPUT • ≥30 Million reads per sample recommended Turn Around Time Data QC • Typical 40 working days from sample QC acceptance to filtered raw data availability • Expedited services are available, contact your local BIOINFORMATICS ANALYSIS BGI specialist for details

Locations Delivery QC BGI’s sequencing services are available from a variety of BGI service laboratory locations, including:

- Copenhagen, Denmark - Philadelphia, USA - Hong Kong, China BGI also has a network of laboratories across mainland China. BGI RNA-SEQ (TRANSCRIPTOME) SEQUENCING

Data Analysis Request Information or In addition to raw data output, BGI offers a range of standard and customized Quotation bioinformatics pipelines for your Transcriptome sequencing project. Contact your BGI account Reports and output data files are delivered in industry standard file formats: FASTQ, representative for the most affordable BAM, FPKM calculation and Excel. rates in the industry, to discuss how we can meet your specific project requirements or for expert advice on STANDARD ANALYSIS experiment design, from sample to bioinformatics. • Filtering • Alignment [email protected] • Quantitative expression profiles • Detection of differentially expressed genes • Spliced transcript analysis International Head Offices

• Fusion gene analysis United States • SNP and Indel detection One Broadway, 3rd Floor • RNA editing analysis Cambridge, MA 02142, USA • Gene ontology analysis Tel: +1 617 500-2741 • Pathway enrichment analysis • Hierarchical clustering analysis Europe Ole Maaløes Vej 3, • Additionally, for Whole Transcriptome Sequencing: lncRNA analysis included DK-2200 Copenhagen N, Denmark Tel: +45 7026 0806 CUSTOMIZED ANALYSIS Asia Pacific Further customization of Bioinformatics analysis to suit your unique project is 16 Dai Fu Street, available: Tai Po Industrial Estate, New Territories, Hong Kong Please contact your BGI technical representative Tel: +852 36103510

Sample Requirements We can process your total RNA, blood, cell line, FFPE, fresh frozen tissue and single cell samples from a variety of species, with the following general requirements:

Sample Concentration For BGI’s scientiﬁc publications Species Amount RIN Value OD Type (ng/µl) relating to RNA-Seq (Transcriptome)

Human/Animal ≥200ng ≥20 RIN≥7.0 28S/18S≥1.0 Sequencing, sample shipping instructions or sample submission Fungi ≥1ug ≥40 RIN≥6.5 28S/18S≥1.0 forms, please visit our website. Total OD ≥1.8, Plant ≥200ng ≥20 RIN≥6.0 28S/18S≥1.0 260/280 RNA OD260/230 ≥1.8 Prokaryotes ≥1ug ≥40 RIN≥7.0 23S/16S≥1.0

Insect ≥200ng ≥20 N/A N/A www.bgi.com

Copyright© 2017 BGI. The BGI logo is a trademark of BGI. All rights reserved. All brand and product names are trademarks or registered trademarks of their respective holders. Information, descriptions, and speciﬁcations in this publication are subject to change without notice. Published January 2017, version 1.0 Services are for research use only.