Genetic and Functional Diversity of Agricultural Microorganisms in Conjunction with the 10Th International Congress for Culture Collections
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The 12th NIAS International Workshop on Genetic Resources Genetic and Functional Diversity of Agricultural Microorganisms In conjunction with the 10th International Congress for Culture Collections ll-16 OCTOBER 2004 TSUKUBA, JAPAN National Institute of Agrobiological Sciences Genetic and Functional Diversity of Agricultural Microorganisms Proceedings of the 12th NIAS International Workshop on Genetic Resources Edited by KURISAKI, Jun-ichi KAKU, Hisatoshi SATO, Toyozo AOKI, Takayuki and NAGAI, Toshirou Published by National Institute of Agrobiological Sciences, Tsukuba, Japan 3 1 January 2005 Printed by Asahi Printing Co., Ltd, Tsukuba, Japan ISBN 4-931511-11-2 CONTENTS Introduction to the workshop Jun-ichi Kurisaki and Taro Obata 1. Genetic and functional diversity of agricultural microorganisms Preface Hisatoshi Kaku and Kerry O 'Donnell Mobile genetic elements contribution to the differentiation of closely related Xanthomonas genomes Marie-Anne VanSluys and Claudia B. Monteiro-Vitorello Species recognition and identification of agriculturally important fiisaria: current status and future prospects Kerry O 'Donnelly DavidM Geiser and Takayuki Aoki Functional analysis for pathogenicity-related genes of xanthomonads using mutagenesis approach Chaozu He and Wei Qian Genetic diversity of Ralstonia solanacearum strains in Japan and the Southeast Asian countries Kenichi Tsuchiya, Mitsuo Honta, WongBoonsuebsakul, Josehto, E. Villa and Karden Mulya Taxonomic analyses of soybean SDS and dry bean root-rot pathogens based on a MAFF - NRRL collaboration Takayuki Aoki and Kerry O 'Donnell Systems biology initiatives in the rice blast fungus, Magnaporthe grisea Yong-HwanLee 2. Newparadigms of biological resource centers (Reproduced by permission of ICCC- 10 Secretariat) Preface Hideaki Sugawara, Makoto M WatanabeandJean Swings 39 The new paradigm of the Biological Resource Centres (BRCs) challenges and opportunities of culture collections or BRCs J. Swings and V.Storms 40 The critical role of Biological Resource Centers in public health RaymondH. Cypess and Shung-Chang Jong 43 National Biological Resource Center to establish the intellectual infrastructure for life sciences and biotechnology Ken-ichiro Suzuki 49 Microbial diversity and pharmaceutical industry culture collections Dwight Baker 5 6 Biosafety demands and the self-image of modern BRCs - Global challenges Christine Rohde 62 3. Progress in the Research on Agricultural Microorganisms Overview of the MAFF Genebank project Toyozo Sato, Toshirou Nagai, Keisuke Tomioka, Kasumi Takeuchi, Motoko Iida and MasaeKawada 69 Current status and future prospects of collection and use of microbial resources in ACCC Ruibo Jiang, Jingang Gu, Bingquan Fan, Xiaoxia Zhang, Xiaotong Ma,Shigui Li and Zhiyong Ruan 7 1 Collection and identification of plant pathogenic Fusarium in Thailand Apirusht Somnth, Pattana Sontirat, Niyom Khaimook and Tharntip Bhasabutra. 74 Maintenance of microbial genetic resources by cryopreservation and freeze-drying Toshirou Nagai, Toyozo Sato, Keisuke Tomioka, KasurniTakeuchi, Motoko Iida and MasaeKawada 76 Long term preservation for 20 years of entomogenous fungi Sanae Wada,Ritsuko Murakami, Wataru Mitsuhashi, Kazuhisa Miyamoto and Kiyoshi Kawakami 78 Grouping of bacteria isolated from leaf sheaths and panicles of intact rice plants Hirosuke Shinohara, Jun-ichiro Enya, Shigenobu Yoshida, Takao Tsukiboshi and Seiya Tsushima 80 Pathogenic diversity ofXanthomonas oryzae pv. oryzae in Myanmar Thein Lwin and Seint San Aye 82 Phylogenetic analysis ofXanthomonas species based on the nucleotide sequences of23S rRNA gene and 16S-23S rDNA spacer region Hirokazu Ochiai, Jean Swings and Hisatoshi Kaku 86 Development of genome database system for Xanthomonas oryzae pv. oryzae Masaru Takeya, Hirokazu Ochiai, Masahiro Satoh and Hisatoshi Kaku 8 8 PCR-based genomovar identification and characterization of Burkholderia cepacia complex strains isolated from diverse origins Kenichi Tsuchiya, Sang-Tae Seo, Chika Fujitam, Hiroyuki Sawada, Takanobu Yoshida and MamiTakahashi 90 Flagellin glycosylation island in Pseudomonas syringae Kasumi Takeuchi, Fumiko Taguchi, Chihiro Yasuda, Hanae Kaku, Katsuyosh Murata, Etsuko Katoh, Yoshishige Inagaki, Kazuhiro Toyoda, TomononShiraishi and Yuki Ichinose 92 Huanglongbing disease management in citrus in Indonesia-Current research Siti Subandiyah, Andi Trisyono, Susamto Somowiyarjo, Andrew Beattie, Paul Holford, Zamir Hossain and Paul De Barro 94 A preliminary study of a toxin associated with Huanglongbing disease on citrus Siti Subandiyah, Rina Ediati, Achmad Himawan,Andi Trisyono, Arman Wijonarko, Zamir Hossain, Paul Holford and Toru Iwanami 97 Phylogeny and taxonomy of bacterial nitrogen-fixing legume symbionts Hiroyuki Sawada, Toshiki Uchiumi, Mikiko Abe, Masakito Hayatsu, Yukan Ichiman, Katsumi Akutsu, Takanobu Yoshida and Kenichi Tsuchiya 100 Gene expression controlled by a conidiophore patterning regulator Acr l in Magnaporthe grisea Marie Nishimura 102 Study on durability of resistance genes to blast disease (Pyricularia grisea) in the Mekong delta Pham VanDu andLe CamLoan 104 Current status of asparagus diseases in Southeast Asia (SEA) Baharuddin Salleh, Siti Nurdijati, Fachri Djas, Pangeran Insanul, Kasmal and Lahmuddin 1 08 Taxonomic and phylogenic study on Bipolaris fiingi from Japan and their productivity of ophiobolins Takao Tsukiboshi, WenHsin Chung and Shigenobu Yoshida 1 1 1 Studies on phytopathological, morphological and molecular variations of Plectosporium tabacinum in Japan Toyozo Sato, Jun Takeuchi, Hideyuki Nagao and Keisuke Tomioka 11 3 Morphology and molecular taxonomy of Colletotrichum destructivum (Teleomorph: Glomerella glydnes) and related species Jouft Moriwaki, Toyozo Sato, Takao Tsukiboshi, Masako Noguchi and Kazuyuki Hirayae 1 15 Pathogenicity of some Colletotrichum species to petals of Antirrhinum majus Keisuke Tomioka, Jouji Moriwaki and Toyozo Sato 1 1 7 Virulence of Fusicoccum aesculi, Phomopsis phomoides, Fusarium lateritium and Stemphylium lycopersici to sweet pepper fruits Keisuke Tomioka and Toyozo Sato 1 1 9 Saccharomyces cerevisiae genome-wide mutant screen for antifungal activities of yeasts, Williopsis mrafcii, Kluyveromyces lactis and S. cerevisiae Hiroko Kuze Kitamoto 12 1 Isolation and characterization of bacteria in fermented vegetables sold in northern part of Vietnam Yasuhiro Inatsu and Shinichi Kawamoto 123 A newmethod to obtain plasmid variants from Lactococcus lactis Miho Kobayashi, Masaru Nomuraand Hiromi Kimoto-Nira 125 Effect of plasmids ofLactococcus strains on cytokine production from murine macrophage cells Hiromi Kimoto-Nira, Miho Kobayashi, Koko Mizumachi, Jun-icki Kurisaki and Takashi Okamoto 127 Effects of a probiotic strain on the cellular immuneresponse to food antigens in mice Koko Mizumachit Hiromi Kimoto and Jun-ichi Kurisaki 1 29 Training course Schedule and participants 1 3 1 Introduction to the workshop Jun-ichi Kurisaki and Taro Obata National Institute of Agrobiological Sciences The National Institute of Agrobiological Sciences (NIAS) holds an International Workshop on Genetic Resources annually. The objectives of these workshops are to exchange research information with experts from around the world and to promote international collaboration for the development of technology related to the evaluation, conservation and use of plant, animal and microorganism genetic resources These workshops have helped to stimulate activities of our genebank system in Japan and help Japanese genetic resources scientists to forge collaborative linkages with scientists in other countries This year we are holding the 12th NIAS International Workshop on Genetic Resources This workshop consisted of two themes 1 Current research on the genetic and functional diversity of microorganisms related to agriculture ; 2. Ways to improve the quality and use of culture collections in the light of developments in microbial genomics and trends related to the Convention on Biological Diversity. This workshop was held in conjunction with the 10th International Congress for Culture Collections (ICCC-10) in Tsukuba during October 2004. The theme of ICCC-10 was "Innovative roles of biological resources centers" This book contains the proceedings of the 12th NIAS International Workshop on Genetic Resources and consists of four parts The first two parts are the papers presented at the two workshop symposia organized under the titles "Genetic and functional diversity of agricultural microorganisms" and "Newparadigms of biological resources centers" In addition, the papers based on the poster presentations are included. At the time of the workshop, a training course was held for Southeast Asian microbiologists in order to help improving their culture collection techniques A report on this training course is provided in this book. Wegreatly appreciate the inputs of scientists from around the world and Japan that helped to make this multi-objective workshop a success We hope that this workshop will stimulate further international developments and research cooperation on culture collections for agricultural microorganisms The 12th NIAS International Workshop on Genetic Resources Organizing Committee OBATA, Taro (Chairperson) Genome and Biodiversity Division KURISAKI, Jun-ichi Genetic Diversity Department OKUNO, Kazutoshi Genebank NAKAMOTO,Yasutoshi : Genebank Executive Committee KAKU, Hisatoshi (Chairperson) Genetic Diversity Department SATO, Toyozo: Genebank HAYASHI, Nagao: Genetic Diversity Department AOKI, Takayuki : Genetic