Supplemental Data For: Zdunić, G., J.E

Supplemental Data for: Zdunić, G., J.E. Preece, G.S. Dangl, A. Koehmstedt, A. Mucalo, E. Maletić, and I. Pejić. 2013. Genetic characterization of grapevine cultivars collected throughout the Dalmatian region. Am. J. Enol. Vitic. 64:285-290. doi: 10.5344/ajev.2012.12085.

Supplemental Table 1 Grape cultivars (Vitis vinifera L.) analyzed in this study. Collecting location/ Registered in the official Cultivar/accession name Berry colora Main useb source Croatian cultivar listd Babić N W Primošten + Babica N W Kaštela + Bak N W Omiš - Beretinjok B W FAZc - Bogdanuša B W FAZc + Bratkovina Blatska B W Island of Korčula + Bratkovina Čara B W Island of Korčula + Cetinka B W Island of Korčula + Cipar Rs W/T FAZc - Crljenak Kaštelanski N W Kaštela + Crljenak Viški N W Island of Vis - Crna Brajda N W Nin - Crnka N W FAZc + Debit B W Nin + Dobričić N W Island of Šolta + Drnekuša Vela N W Island of Hvar + Frmentum B W FAZc - Gegić B W FAZc + Glavanjuša N W Island of Vis + Glavinuša Kaštela Rg W Kaštela + Gnjatonja B W Nin - Grk B W Island of Korčula + Gustopupica Ninska Rg W FAZc + Krivaja Bijela B T Island of Vis - Krivaja Crvena Rg T Island of Vis - Krkošija B W FAZc + Krstičevica B W Island of Vis + Kujundžuša B W Imotski + Lasina N W FAZc + Linčuša N W Omiš + Ljutun N W Kaštela + Malvasija Dubrovačka B W FAZc + Malvazija Istarska B W Kaštelir, Poreč + Maraština Omiš B W Omis - Marinkovića Grozje B T FAZc - Mijajuša Rg W FAZc - Mladenka B W Kaštela + Muskat Ruža Omiški RgV W/T Omiš + Ninčuša N W Kaštela + Okatac Omiški Rg W Omiš + Okatac Omiški Rodni Rg W Omiš + Okatica Bijela B W Imotski + Okatica Crna N W Imotski + Palagružanka B W Island of Palagruža - Palaruša B W Omiš + Pavicić N W Island of Vis + Plava Lovora N W Pelješac - Plava Lovora Rodna N W Pelješac - Plavac Mali N W Island of Hvar + Plavac Mali Sivi G W Pelješac - Plavina Crna N W FAZc + Plavina Crna Sitna N W Zemunik + Pošip Crni Rg W FAZc - Pošip Bijeli B W Island of Korčula + Pošip Bijeli Sitni B W Island of Korčula + Pribidrag N W Omiš + Rogoznička N W Kaštela + Rudežuša N W Imotski + Rukatac B W Island of Korčula + Šarica Trišnjavica Rg W FAZc - Silbijanac B W/T FAZc + Svrdlovina N W Zemunik + Tanetova B W Island of Vis - Terin N W Nin - Unknown IJK10 Rg W Omiš - Vlaška B W Kaštela + Vranac N W FAZc + Vrškajica B W Pelješac - Vugava B W Island of Vis + Vugava Bijela Omiška B W Omiš - Vugava Crvena Omiška Rg W Omiš - Zadarka N W FAZc + Zelenka Šolta B W Island of Šolta - Žilavka B W FAZc + Zlatarica Blatska B W FAZc + Zlatarica Vrgorska B W FAZc + aAccording to OIV code 225: B (green-yellow), G (grey), Rs (rose), Rg (red), N (blue-black), RgV (red-violet). bW: wine cultivar, T: table cultivar for fresh consumption, W/T: dual type. cPropagated from cuttings obtained from Faculty of Agriculture, University of Zagreb. dOfficial Regulation on the National Croatian List of Recognized Vine Varieties (NN 159/04). 1 Am. J. Enol. Vitic. Supplemental Data for: Zdunić, G., J.E. Preece, G.S. Dangl, A. Koehmstedt, A. Mucalo, E. Maletić, and I. Pejić. 2013. Genetic characterization of grapevine cultivars collected throughout the Dalmatian region. Am. J. Enol. Vitic. 64:285-290. doi: 10.5344/ajev.2012.12085.

Supplemental Table 2 SSR profiles of 63 presumed native Dalmatian cultivars maintained at Institute for Adriatic Crops in Split and five reference cultivars at 11 microsatellite loci. Allele sizes given in base pairs (bp) (nd: not detected). Accession VVS2 VVMD5 VVMD7 VVMD27 VrZAG62 VrZAG79 VVMD25 VVMD28 VVMD32 VVMD31 VVMD34 Babić 143 151 228 228 247 249 179 179 205 205 237 259 243 243 247 251 241 253 212 216 240 248 Babica 143 153 228 228 239 249 179 179 189 191 259 259 243 245 247 251 253 265 212 212 240 242 Bak 143 143 226 232 239 251 179 181 189 205 259 259 243 259 261 261 257 273 212 212 240 248 Beretinjok 143 145 232 232 239 253 181 185 189 201 243 259 245 259 239 249 259 273 204 212 240 242 Bogdanuša 143 151 222 228 239 249 183 194 189 191 247 251 245 263 251 261 253 259 210 212 240 248 Bratkovina Blatska 133 135 234 240 239 247 179 181 187 205 249 249 243 259 247 249 251 253 204 212 242 248 Cetinka 133 135 226 226 239 243 181 189 189 189 257 259 245 259 239 239 263 265 204 210 240 248 Cipar 133 143 228 236 247 253 185 185 189 195 239 251 245 259 239 261 257 263 216 216 240 248 Crljenak Kaštelanski 133 143 226 236 247 249 179 181 201 205 237 259 243 243 251 261 257 265 212 214 240 242 Crljenak Viški 133 135 226 240 239 249 179 181 187 201 251 259 243 259 247 251 251 257 204 214 240 248 Crna Brajda 145 153 226 232 247 249 179 181 191 203 249 259 245 245 251 281 253 273 210 212 240 240 Crnka 133 143 228 246 239 249 179 194 187 205 237 249 243 243 239 261 253 273 204 212 240 248 Debit 133 145 228 228 239 249 179 194 191 197 237 251 243 245 239 251 241 273 214 214 224 240 Dobričić 145 151 228 228 247 249 179 179 191 205 237 259 243 245 251 261 253 265 212 216 240 242 Drnekuša Vela 133 145 228 240 247 249 179 194 189 191 251 259 245 259 251 261 257 273 204 212 240 248 Frmentum 143 145 226 234 249 253 179 185 189 203 237 251 245 259 239 261 241 253 212 212 240 248 Gegić 133 145 226 228 247 253 179 194 195 201 237 251 243 245 247 247 251 273 214 216 240 240 Glavinuša Kaštela 133 145 228 240 239 249 179 181 189 205 259 259 243 245 231 251 nd nd 212 212 240 240 Gnjatonja 133 143 228 234 239 249 179 181 189 201 243 249 243 245 255 261 251 257 204 212 240 240 Grk 133 133 234 236 247 249 179 179 205 205 237 251 243 245 247 261 257 273 212 212 242 248 Gustopupica Ninska 143 143 226 226 239 243 179 179 189 197 237 251 243 259 249 261 251 273 210 212 240 240 Krivaja Bijela 133 149 238 246 249 249 179 187 189 205 251 251 249 253 247 261 257 263 214 214 240 240 Krivaja Crvena 145 149 238 246 239 249 179 179 189 205 251 251 nd nd 261 261 257 257 212 214 240 240 Krkošija 133 153 226 230 239 239 179 194 189 197 249 249 243 245 255 255 273 273 212 214 240 242 Krstičevica 143 145 228 232 239 253 179 181 189 189 237 259 243 245 nd nd 253 259 212 212 240 240 Kujundžuša 133 143 222 226 239 247 179 189 189 205 245 255 nd nd 237 261 241 273 210 212 240 240 Lasina 133 133 228 246 233 239 179 181 197 197 237 249 243 243 239 249 241 257 212 214 240 248 Linčuša 143 145 226 228 249 249 179 194 191 191 237 251 243 243 251 261 257 273 212 212 224 240 Ljutun 145 151 228 228 247 253 179 194 201 205 251 259 245 263 247 261 257 259 212 212 224 240 Malvasija Dubrovačka 143 145 226 226 243 249 179 183 189 203 243 247 245 259 239 261 253 273 210 212 240 248 Malvazija Istarska 143 143 222 240 239 239 179 179 189 189 237 255 243 259 255 281 257 273 204 210 240 248 Maraština Omiš 133 145 226 246 243 247 189 189 189 197 251 257 245 245 237 261 263 273 210 212 240 240 Marinkovića Grozje 133 135 226 232 239 249 185 185 187 189 243 251 253 259 237 261 259 273 204 212 240 240 Mijajuša 133 143 234 246 233 249 179 179 189 205 247 247 243 249 247 261 251 273 210 214 240 240 Mladenka 145 151 226 228 247 253 179 194 201 205 237 251 243 263 247 247 265 273 212 212 240 242 Muškat Ruža Omiški 135 149 232 238 247 249 179 185 187 193 239 255 253 259 239 247 273 273 212 216 240 240 Okatica Bijela 151 151 226 228 247 249 181 194 191 205 249 259 253 263 239 247 273 273 210 212 nd nd Okatica Crna 133 151 226 236 245 247 179 189 195 205 245 251 245 253 237 247 241 251 212 216 240 248 Palagružanka 143 145 226 228 247 249 179 194 197 201 249 257 245 259 251 261 259 265 210 214 248 248 Palaruša 133 137 228 240 249 253 181 183 203 203 245 259 243 243 237 261 253 273 212 214 240 248 Pavicić 143 151 226 226 247 249 179 194 191 205 249 259 243 253 239 251 257 273 210 212 240 240 Plava Lovora 145 151 228 228 249 253 179 181 191 201 237 259 245 263 251 251 257 273 212 212 240 248 Plavac Mali 143 145 226 228 247 249 179 179 191 205 237 259 243 245 251 261 253 257 212 212 240 240 Plavina Crna 133 143 232 236 239 249 179 189 189 189 237 243 243 243 251 261 253 265 212 214 240 242 Pošip Crni 135 135 nd nd 239 239 179 181 187 189 251 259 259 259 239 247 253 265 204 204 242 248 Pošip Bijeli 133 133 228 240 239 239 179 181 187 189 251 259 243 245 247 249 253 257 204 204 242 248 Rudežuša 133 143 226 228 239 253 179 181 189 201 251 259 243 259 231 237 251 253 212 212 240 240 Rukatac 145 145 226 240 239 253 179 179 197 201 243 251 243 245 251 255 253 257 212 214 240 242 Šarica Trišnjavica 145 151 nd nd 243 247 189 189 189 205 249 251 243 243 247 261 241 273 212 216 240 248 Silbijanac 151 153 226 228 239 239 179 179 189 189 249 255 245 263 247 281 241 265 204 212 240 242 Svrdlovina 133 153 238 240 247 249 181 181 195 201 257 259 245 259 261 281 251 273 212 212 240 240 Terin 143 153 226 228 239 247 179 194 189 205 249 255 245 259 237 247 nd nd 204 210 240 240 Unknown IJK-10 133 135 228 228 239 249 179 181 197 205 237 259 259 263 231 251 nd nd 212 214 240 242 Vlaška 143 153 226 240 239 253 179 179 189 201 251 259 245 259 251 255 257 265 212 212 240 242 Vranac 133 133 226 226 247 249 181 181 195 201 259 259 243 245 239 251 257 257 214 216 240 240 Vrškajica 133 133 226 232 239 239 179 181 189 197 237 259 243 243 247 251 257 265 212 212 240 242 Vugava 133 151 240 240 239 249 179 181 189 189 251 259 243 263 231 255 257 273 212 212 240 240 Vugava Bijela Omiška 135 145 226 228 239 249 179 181 189 191 259 259 243 259 239 261 nd nd 204 212 240 248 Vugava Crvena Omiška 133 143 226 226 239 245 181 194 189 195 249 259 243 243 239 261 nd nd 204 216 240 240 Zadarka 133 143 226 234 239 247 179 194 189 203 249 255 243 259 255 281 253 257 210 212 240 240 Žilavka 133 151 228 240 239 249 181 189 189 201 245 251 243 263 237 239 253 273 212 212 240 248 Zlatarica Blatska 133 153 226 228 239 239 181 194 189 189 257 259 245 245 247 251 257 273 204 212 242 248 Zlatarica Vrgorska 135 143 226 232 239 249 179 181 189 195 251 259 243 259 249 251 257 273 212 212 240 248 References Cabernet Sauvignon 139 151 232 240 239 239 175 189 189 195 247 247 243 253 237 239 241 241 206 210 240 248 Chardonnay 137 143 234 238 239 243 181 189 189 197 243 243 243 259 221 231 241 273 214 216 240 240 Pinot noir 137 151 228 238 239 243 185 189 189 195 239 245 243 253 221 239 241 273 216 216 240 240 Sangiovese 133 133 226 236 239 263 179 185 195 197 243 259 245 245 237 247 253 257 212 212 240 240 Zinfandel 133 143 226 236 247 249 179 181 201 205 237 259 243 243 251 261 257 265 212 214 240 242

2 Am. J. Enol. Vitic. Supplemental Data for: Zdunić, G., J.E. Preece, G.S. Dangl, A. Koehmstedt, A. Mucalo, E. Maletić, and I. Pejić. 2013. Genetic characterization of grapevine cultivars collected throughout the Dalmatian region. Am. J. Enol. Vitic. 64:285-290. doi: 10.5344/ajev.2012.12085.

Supplemental Table 3 Genetic diversity parameters for the 11 SSR loci analyzed based on the 63 unique genotypes from 76 grapevines accessions from the Institute for Adriatic Crops and Karst Reclamation in Split. He: expected heterozygosity, Ho: observed heterozygosity, r: probability of null alleles, PI: probability of identity, PIC: polymorphism information content. Alleles Allele size Locus (n) range (bp) He Ho r PI PIC VVS2 8 133-153 0.799 0.825 -0.015 0.069 0.771 VVMD5 10 222-246 0.790 0.754 0.020 0.071 0.762 VVMD7 8 233-253 0.750 0.857 -0.061 0.103 0.710 VVMD27 7 179-194 0.692 0.714 -0.013 0.135 0.651 VrZAG62 9 187-205 0.805 0.810 -0.002 0.060 0.783 VrZAG79 10 237-259 0.822 0.841 -0.011 0.054 0.800 VVMD25 6 243-263 0.727 0.770 -0.025 0.119 0.682 VVMD28 9 231-281 0.840 0.887 -0.025 0.045 0.821 VVMD32 8 241-273 0.819 0.914 -0.052 0.056 0.796 VVMD31 5 204-216 0.660 0.635 0.015 0.150 0.625 VVMD34 4 224-248 0.541 0.629 -0.057 0.263 0.489 All loci 84 2.55x10-12 Mean 7.6 0.749 0.785 0.717

Supplemental Figure 1 Distribution and relative frequency of microsatellite alleles in 63 grapevine genotypes from Dalmatia analyzed at 11 SSR loci.

3 Am. J. Enol. Vitic.