DOCSLIB.ORG

6311.Full.Pdf

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
6311.Full.Pdf Role of p190RhoGAP in β2 Integrin Regulation of RhoA in Human Neutrophils Karim Dib, Fredrik Melander and Tommy Andersson This information is current as J Immunol 2001; 166:6311-6322; ; of September 26, 2021. doi: 10.4049/jimmunol.166.10.6311 http://www.jimmunol.org/content/166/10/6311 Downloaded from References This article cites 53 articles, 34 of which you can access for free at: http://www.jimmunol.org/content/166/10/6311.full#ref-list-1 Why The JI? Submit online. http://www.jimmunol.org/ • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average by guest on September 26, 2021 Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2001 by The American Association of Immunologists All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. ␤ Role of p190RhoGAP in 2 Integrin Regulation of RhoA in Human Neutrophils1 Karim Dib,2 Fredrik Melander, and Tommy Andersson ␤ We found that engagement of 2 integrins on human neutrophils induced activation of RhoA, as indicated by the increased ratio ␤ ؉ of GTP:GTP GDP recovered on RhoA and translocation of RhoA to a membrane fraction. The clustering of 2 integrins also ␤ induced a time-dependent increase in GDP bound to RhoA, which correlated with 2 integrin-induced activation of p190RhoGAP. The activation of p190RhoGAP was completely blocked by [4-amino-5-(4-methylphenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine] ␤ (PP1), a selective inhibitor of Src family tyrosine kinases. However, clustering of 2 integrins did not increase the basal tyrosine ␤ phosphorylation of p190RhoGAP, nor did it affect the amount of p120RasGAP bound to p190RhoGAP. Instead, the 2 integrin- induced activation of p190RhoGAP was accompanied by increased tyrosine phosphorylation of a p190RhoGAP-associated protein, ␤ p120RasGAP, and accumulation of both p120RasGAP and p190RhoGAP in a membrane fraction. PP1 blocked the 2 integrin- Downloaded from induced phosphorylation of p120RasGAP, as well as the translocation of p190RhoGAP and p120RasGAP, but it did not affect the accumulation of RhoA in the membrane fraction. In agreement with the mentioned findings, PP1 also increased the GTP:GTP ؉ ␤ ␤ GDP ratio recovered on RhoA immunoprecipitated from 2 integrin-stimulated cells. Thus, in neutrophils, 2 integrin-induced activation of p190RhoGAP requires a signal from a Src family tyrosine kinase, but it does not occur via the signaling pathway responsible for activation of RhoA. The Journal of Immunology, 2001, 166: 6311–6322. http://www.jimmunol.org/ ␤ embers of the 2 integrin family are the dominating long to the Rho subfamily, members of the Ras superfamily of integrins expressed on neutrophils, and they play a small GTPases, have been strongly implicated (11). These small M major role in cell-cell and cell-matrix adhesion of neu- GTP-binding proteins cycle between a GDP-bound inactive form ␤ trophils. 2 integrins are noncovalently associated heterodimers to a GTP-bound active form (12). Guanine nucleotide exchange composed of a common ␤-chain, CD18, and one of four unique factors (GEFs),3 activated by extracellular stimuli, are responsible ␣-chains; CD11a, CD11b, CD11c, or CD11d, with CD11b/CD18 for the GDP-GTP exchange, a transition that allows translocation being the most prominent on neutrophils (1, 2). The signal trans- of the monomeric G proteins to the plasma membrane (13, 14). In duction properties of these integrins are vital for the migration of their GTP-bound state, these proteins interact with specific effec- neutrophils, but to date have only been partly elucidated. tors to initiate downstream signals and functions. The subsequent by guest on September 26, 2021 ␤ Engagement of 2 integrins on neutrophils has been accom- hydrolysis of bound GTP to GDP is mediated via the intrinsic plished in experimental models by plating the cells on surface- GTPase activity of these GTP-binding proteins, which, however, is bound anti-␤ integrin Abs or on ICAM-1 or other types of coated 2 so low that the proteins are totally dependent on GTPase-activating surfaces. The results show that ␤ integrin engagement triggers 2 proteins (GAPs) to achieve adequate hydrolysis of GTP (12). various signal transduction events, including tyrosine phosphory- The best-characterized Rho-specific GAP is p190RhoGAP, lation of several different proteins (3–8), activation of the Src fam- which was originally reported to be a tyrosyl-phosphorylated pro- ily tyrosine kinases p59/61hck and p58c-fgr (3, 9), and activation of tein that coprecipitated with p120RasGAP (15). Molecular cloning p21ras (7). These signals are associated with a profound alteration of the cognate cDNA of p190RhoGAP has revealed an NH -ter- in cell morphology that leads to cell spreading and locomotion. It 2 has been reported that these morphological changes are driven by minal region that contains several sequence motifs that are shared ␤ by all known GTP-binding proteins, and a C-terminal region with dynamic 2 integrin-induced rearrangement of the actin cytoskel- eton (10), but little is known about the nature of the signal(s) a GAP domain specific for the GTP-binding proteins Rho and Rac ␤ (16). Several lines of evidence support the idea that p190RhoGAP generated by 2 integrins regulating cytoskeletal rearrangements. However, in other cell types, small GTP-binding proteins that be- somehow participates in rearrangement of actin cytoskeleton. For example, microinjection of the C-terminal region of p190RhoGAP into Swiss 3T3 cells has been found to block serum-induced stress Division of Experimental Pathology, Department of Laboratory Medicine, Lund Uni- fiber formation (17). McGlade et al. (18) reported that constitutive versity, Malmo¨University Hospital, Malmo¨, Sweden association of GAP-N (i.e., p120RasGAP lacking the C-terminal Received for publication May 5, 2000. Accepted for publication March 7, 2001. Ras-binding domain) with p190RhoGAP increased the GAP ac- The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance tivity of this complex, caused a subsequent cellular loss of actin with 18 U.S.C. Section 1734 solely to indicate this fact. stress fibers, and reduced cellular adhesion. In adherent melanoma 1 ␤ This work was supported by the Swedish Cancer Foundation, the Network for In- cells, Nakahara et al. (19) found that engagement of 1 integrins flammation Research (funded by the Swedish Foundation for Strategic Research), the Universitetssjukhust-Malmo¨ Alma¨nna Sjukhus Research Foundations, the Medical Faculty of Lund University, The King Gustaf V Memorial Foundation, The Crafoord Foundation, The O¨ sterlund Foundation, and The Koch Foundation. 2 Address correspondence and reprint requests to Dr. Karim Dib, Department of Lab- 3 Abbreviations used in this paper: GEF, guanine nucleotide exchange factor; FN, oratory Medicine, Division of Experimental Pathology, Lund University, Malmo¨Uni- fibrinogen; GAP, GTPase-activating protein; PP1, [4-amino-5-(4-methylphenyl)-7-(t- versity Hospital, Entrance 78, SE-205 02 Malmo¨, Sweden. E-mail address: butyl)pyrazolo[3,4-d]pyrimidine]; RAM, rabbit anti-mouse; EGF, epidermal growth [email protected] factor; PVDF, polyvinylidene difluoride. Copyright © 2001 by The American Association of Immunologists 0022-1767/01/$02.00 ␤ 6312 2 INTEGRIN REGULATION OF p190RhoGAP induced increased phosphorylation of p190RhoGAP, which en- were washed twice with PBS and then incubated with casein (2.0 mg/ml) abled association with filamentous actin. In addition, it was dem- for 2 h. Thereafter, the plates were washed twice with PBS and anti-CD18 ␮ onstrated (20) that increased tyrosine phosphorylation of (IB4) Ab (10 g/ml) or the isotype-matched negative control IgG2a Ab (10 ␮g/ml) was added, and the plates were incubated overnight. Before use, the p190RhoGAP correlated with rapid and exaggerated disassembly plates were washed and incubated with 10% heat-inactivated FCS for 2 h of actin stress fibers. The cited authors suggested that the intrinsic and then washed extensively (three times with PBS and twice with the ␤ RhoGAP activity of p190RhoGAP might be augmented by phos- calcium-containing medium). 2 integrins on neutrophils were engaged by phorylation, but they have not tested that possibility. adding the cells to the coated dishes at 37°C and incubating for different periods of time. Alternatively, neutrophils were plated on a surface coated In this paper, we investigated the signal transduction pathways with FN (20 ␮g/ml) in the presence of TNF-␣ (20 ng/ml), a procedure that involved in ␤ integrin-mediated regulation of RhoA activity in ␤ ␤ 2 specifically activates 2 integrins on neutrophils (24). As a control for 2 neutrophils. In particular, we researched whether a relationship integrin-independent spreading of neutrophils, we also plated these cells on exists between activation of p190RhoGAP and RhoA. anti-CD59 Ab-coated surfaces (the method
Load more

Recommended publications
  • TNF Cdc42-Mediated Filopodium
    Multiple Signal Transduction Pathways Regulate TNF-Induced Actin Reorganization in Macrophages: Inhibition of Cdc42-Mediated Filopodium Formation by This information is current as TNF of September 28, 2021. Maikel Peppelenbosch, Elke Boone, Gareth E. Jones, S.J.H. van Deventer, Guy Haegeman, Walter Fiers, Johan Grooten and Anne J. Ridley J Immunol 1999; 162:837-845; ; Downloaded from http://www.jimmunol.org/content/162/2/837 References This article cites 48 articles, 17 of which you can access for free at: http://www.jimmunol.org/ http://www.jimmunol.org/content/162/2/837.full#ref-list-1 Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists by guest on September 28, 2021 • Fast Publication! 4 weeks from acceptance to publication *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 1999 by The American Association of Immunologists All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. Multiple Signal Transduction Pathways Regulate TNF-Induced Actin Reorganization in Macrophages: Inhibition of Cdc42-Mediated Filopodium Formation by TNF Maikel Peppelenbosch,1*†‡§ Elke Boone,‡ Gareth E. Jones,¶ S.J.H. van Deventer,† Guy Haegeman,‡ Walter Fiers,‡ Johan Grooten,‡ and Anne J.
    [Show full text]
  • Diacylglycerol Kinase Α Mediates HGF-Induced Rac Activation and Membrane Ruffling by Regulating Atypical PKC and Rhogdi
    Diacylglycerol kinase α mediates HGF-induced Rac activation and membrane ruffling by regulating atypical PKC and RhoGDI Federica Chianalea,1, Elena Raineroa,1, Cristina Cianflonea, Valentina Bettioa, Andrea Pighinia, Paolo E. Porporatoa, Nicoletta Filigheddua, Guido Serinib,c, Fabiola Sinigagliaa, Gianluca Baldanzia, and Andrea Graziania,2 aDepartment of Clinical and Experimental Medicine and Biotecnologie per la Ricerca Medica Applicata, University Amedeo Avogadro of Piemonte Orientale, 28100 Novara, Italy; bDepartment of Oncological Sciences and Division of Molecular Angiogenesis, Istituto per la Ricerca e la Cura del Cancro, Institute for Cancer Research and Treatment, University of Torino School of Medicine, 10060 Candiolo, Italy; and cCenter for Complex Systems in Molecular Biology and Medicine, University of Torino, 10100 Torino, Italy Edited* by Lewis Clayton Cantley, Harvard Medical School, Boston, MA, and approved December 14, 2009 (received for review July 29, 2009) Diacylglycerol kinases (DGKs) convert diacylglycerol (DAG) into β-chimaerin (5). Conversely, by generating PA, DGKs regulate phosphatidic acid (PA), acting as molecular switches between several signaling proteins, including serine kinases, small-GTPase- DAG- and PA-mediated signaling. We previously showed that Src- regulating proteins, and lipid-metabolizing enzymes (reviewed in dependent activation and plasma membrane recruitment of DGKα refs. 6 and 7). Thus, by regulating in a reciprocal manner the level of are required for growth-factor-induced cell migration and ruffling, both DAG and PA lipid second messengers, DGK enzymes may act through the control of Rac small-GTPase activation and plasma as terminators of DAG-mediated signals as well as activators of membrane localization. Herein we unveil a signaling pathway PA-mediated ones.
    [Show full text]
  • Rac1 Activation Can Generate Untemplated, Lamellar Membrane Ruffles F
    Leyden et al. BMC Biology (2021) 19:72 https://doi.org/10.1186/s12915-021-00997-3 METHODOLOGY ARTICLE Open Access Rac1 activation can generate untemplated, lamellar membrane ruffles F. Leyden1†, S. Uthishtran2†, U. K. Moorthi2,3†, H. M. York2,3†, A. Patil2,3, H. Gandhi2,3, E. P. Petrov4, T. Bornschlögl5 and S. Arumugam1,2,3,6* Abstract Background: Membrane protrusions that occur on the dorsal surface of a cell are an excellent experimental system to study actin machinery at work in a living cell. Small GTPase Rac1 controls the membrane protrusions that form and encapsulate extracellular volumes to perform pinocytic or phagocytic functions. Results: Here, capitalizing on rapid volumetric imaging capabilities of lattice light-sheet microscopy (LLSM), we describe optogenetic approaches using photoactivable Rac1 (PA-Rac1) for controlled ruffle generation. We demonstrate that PA-Rac1 activation needs to be continuous, suggesting a threshold local concentration for sustained actin polymerization leading to ruffling. We show that Rac1 activation leads to actin assembly at the dorsal surface of the cell membrane that result in sheet-like protrusion formation without any requirement of a template. Further, this approach can be used to study the complex morpho-dynamics of the protrusions or to investigate specific proteins that may be enriched in the ruffles. Deactivating PA-Rac1 leads to complex contractile processes resulting in formation of macropinosomes. Using multicolour imaging in combination with these approaches, we find that Myo1e specifically is enriched in the ruffles. Conclusions: Combining LLSM and optogenetics enables superior spatial and temporal control for studying such dynamic mechanisms. Demonstrated here, the techniques implemented provide insight into the complex nature of the molecular interplay involved in dynamic actin machinery, revealing that Rac1 activation can generate untemplated, lamellar protrusions.
    [Show full text]
  • The Nanomorphology of Cell Surfaces of Adhered Osteoblasts
    The nanomorphology of cell surfaces of adhered osteoblasts C. Voelkner1,2, M. Wendt1,2, R. Lange1,2, M. Ulbrich1,2, M. Gruening1,3, S. Staehlke1,3, J.B. Nebe1,3, I. Barke1,2 and S. Speller*1,2 Address: 1Department Science and Technology of Life, Light and Matter, University of Rostock, Albert-Einstein-Str. 25, 18059 Rostock, Germany. 2Institute of Physics, University of Rostock, Albert-Einstein-Str. 23, 18059 Rostock, Germany. 3Department of Cell Biology, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, Germany. Email: Sylvia Speller – [email protected] * Corresponding author Abstract Functionality of living cells is inherently linked to subunits with dimensions on the nanoscale. In case of osteoblasts the cell surface plays a particularly important role for adhesion and spreading which are crucial properties with regard to bone implants. Here we present a comprehensive characterization of the 3D nanomorphology of living as well as fixed osteoblastic cells using scanning ion conductance microscopy (SICM) which is a nanoprobing method largely avoiding forces. Dynamic ruffles are observed, manifesting themselves in characteristic membrane protrusions. They contribute to the overall surface corrugation which we systematically study by introducing the relative 3D excess area as a function of projected adhesion area. A clear anticorrelation is found upon analysis of ~40 different cells on glass as well as on amine covered surfaces. At the rim of lamellipodia characteristic edge heights between 100 nm and ~300 nm are observed. Power spectral densities of membrane fluctuations show frequency-dependent decay exponents in excess of -2 on living osteoblasts. We discuss the capability of apical membrane features and fluctuation dynamics in aiding assessment of adhesion and migration properties on a single-cell basis.
    [Show full text]
  • Direct Binding of SWAP-70 to Non-Muscle Actin Is Required for Membrane Ruffling
    500 Research Article Direct binding of SWAP-70 to non-muscle actin is required for membrane ruffling Sayoko Ihara, Tsutomu Oka* and Yasuhisa Fukui‡ Division of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan *Present address: Weis Center for Research, Gessinger Clinic, Danville, PA 17822, USA ‡Author for correspondence (e-mail: [email protected]) Accepted 26 October 2005 Journal of Cell Science 119, 500-507 Published by The Company of Biologists 2006 doi:10.1242/jcs.02767 Summary Membrane ruffling induced by growth factor stimulation cells are stimulated with growth factors, suggesting the is caused by actin remodeling, which is mediated by various presence of a stimuli-dependent regulatory mechanism for signaling molecules including Rac. We have shown that actin-binding activity in vivo. Overexpression of the mutant SWAP-70, which binds phosphatidylinositol trisphosphate, SWAP-70 lacking this binding domain inhibits the is one such molecule required for membrane ruffling in membrane ruffling induced by epidermal growth factor mouse kidney cells. Here, we show that SWAP-70 directly stimulation in COS7 cells. This dominant-negative effect is binds to F-actin. The bacterially expressed C-terminal also observed in membrane ruffling induced by a region of SWAP-70 co-sedimented with non-muscle F-actin, dominant-active Rac, suggesting that SWAP-70 cooperates suggesting direct binding of SWAP-70 to F-actin. The with Rac. These results suggest that the binding activity of binding was much weaker in muscle F-actin.
    [Show full text]
  • A Genome-Wide Sirna Screen Implicates Spire1/2 in Sipa-Driven Salmonella Typhimurium Host Cell Invasion
    RESEARCH ARTICLE A Genome-Wide siRNA Screen Implicates Spire1/2 in SipA-Driven Salmonella Typhimurium Host Cell Invasion Daniel Andritschke1, Sabrina Dilling1, Mario Emmenlauer2, Tobias Welz3, Fabian Schmich4,5, Benjamin Misselwitz1,6, Pauli Rämö2, Klemens Rottner7,8, Eugen Kerkhoff3, Teiji Wada9, Josef M. Penninger9, Niko Beerenwinkel4,5, Peter Horvath10, Christoph Dehio2, Wolf-Dietrich Hardt1* 1 Institute of Microbiology, Eidgenössische Technische Hochschule Zurich, CH-8093, Zurich, Switzerland, 2 Biozentrum, University of Basel, CH-4056, Basel, Switzerland, 3 Department of Neurology, University of a11111 Regensburg, DE- 93040, Regensburg, Germany, 4 Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zurich, CH-4058, Basel, Switzerland, 5 SIB Swiss Institute for Bioinformatics, 4058, Basel, Switzerland, 6 Division of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, CH-8091, Zurich, Switzerland, 7 Zoological Institute, Technische Universität Braunschweig, D-38106, Braunschweig, Germany, 8 Department of Cell Biology, Helmholtz Centre for Infection Research, D-38124, Braunschweig, Germany, 9 Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), A-1030, Vienna, Austria, 10 Light Microscopy Center, Eidgenössische Technische Hochschule Zurich, CH-8093, Zurich, Switzerland OPEN ACCESS * [email protected] Citation: Andritschke D, Dilling S, Emmenlauer M, Welz T, Schmich F, Misselwitz B, et al. (2016) A Genome-Wide siRNA Screen Implicates Spire1/2 in SipA-Driven Salmonella Typhimurium Host Cell Abstract Invasion. PLoS ONE 11(9): e0161965.doi:10.1371/ journal.pone.0161965 Salmonella Typhimurium (S. Tm) is a leading cause of diarrhea. The disease is triggered by Editor: Yousef Abu Kwaik, University of Louisville, pathogen invasion into the gut epithelium. Invasion is attributed to the SPI-1 type 3 secretion UNITED STATES system (T1).
    [Show full text]
  • Requirements for Both Rac1 and Cdc42 in Membrane Ruffling And
    CORE Metadata, citation and similar papers at core.ac.uk Provided by PubMed Central Requirements for Both Rac1 and Cdc42 in Membrane Ruffling and Phagocytosis in Leukocytes By Dianne Cox,* Peter Chang,* Qing Zhang,* P. Gopal Reddy,* Gary M. Bokoch,‡ and Steven Greenberg* From the *Pulmonary Division, Department of Medicine, Columbia University College of Physicians and Surgeons, New York 10032; and ‡Department of Immunology and Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037 Summary Specific pathways linking heterotrimeric G proteins and Fcg receptors to the actin-based cyto- skeleton are poorly understood. To test a requirement for Rho family members in cytoskeletal events mediated by structurally diverse receptors in leukocytes, we transfected the full-length human chemotactic peptide receptor in RAW 264.7 cells and examined cytoskeletal alterations in response to the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (FMLP), col- ony stimulating factor–1 (CSF-1), IgG-coated particles, and phorbol 12-myristate 13-acetate (PMA). Expression of Rac1 N17, Cdc42 N17, or the GAP domain of n-chimaerin inhibited cytoskeletal responses to FMLP and CSF-1, and blocked phagocytosis. Accumulation of F-actin– rich “phagocytic cups” was partially inhibited by expression of Rac1 N17 or Cdc42 N17. In contrast, PMA-induced ruffling was not inhibited by expression of Rac1 N17, but was blocked by expression of Cdc42 N17, indicating that cytoskeletal inhibition by these constructs was nonoverlapping. These results demonstrate differential requirements for Rho family GTPases in leukocyte motility, and indicate that both Rac1 and Cdc42 are required for Fcg receptor– mediated phagocytosis and for membrane ruffling mediated by structurally distinct receptors in macrophages.
    [Show full text]
  • Lamellipodin Tunes Cell Migration by Stabilizing Protrusions and Promoting Adhesion Formation
    bioRxiv preprint doi: https://doi.org/10.1101/777326; this version posted September 20, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation Georgi Dimchev1,2, Behnam Amiri3, Ashley C. Humphries4,7, Matthias Schaks1,2, Vanessa Dimchev1,2, Theresia E.B. Stradal2, Jan Faix5, Matthias Krause6, Michael Way4, Martin Falcke3,*, and Klemens Rottner1,2,8,* 1Division of Molecular Cell Biology, Zoological Institute, Technische Universität Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany; 2Department of Cell Biology, Helmholtz Centre for Infection Research, Inhoffen Strasse 7, 38124 Braunschweig, Germany; 3Max Delbrück Center for Molecular Medicine, Robert Rössle Strasse 10, 13125 Berlin, Germany; 4Cellular Signalling and Cytoskeletal Function Laboratory, The Francis Crick Institute, London, England, UK; 5Institute for Biophysical Chemistry, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany; 6Randall Centre of Cell & Molecular Biophysics, King's College London, New Hunt's House, Guy's Campus, London, SE1 1UL, UK; 8Braunschweig Integrated Centre of Systems Biology (BRICS), Braunschweig, D-38106, Germany 7present address: Icahn School of Medicine at Mount Sinai, New York, USA *Authors for correspondence: Klemens Rottner or Martin Falcke Klemens Rottner: Phone: +49 531 391 3255, Email: [email protected] Martin Falcke: Phone: +49 30 9406 2753, Email: [email protected] Running title: Lamellipodin function in protrusion Key words: lamellipodium, VASP, Arp2/3, WAVE regulatory complex Summary statement: We describe how genetic ablation of the prominent actin- and VASP- binding protein lamellipodin combined with software-aided protrusion analysis uncovers mechanistic insights into its cellular function during cell migration.
    [Show full text]
  • Rhoa Inactivation by P190rhogap Regulates Cell Spreading and Migration by Promoting Membrane Protrusion and Polarity William T
    Molecular Biology of the Cell Vol. 12, 2711–2720, September 2001 RhoA Inactivation by p190RhoGAP Regulates Cell Spreading and Migration by Promoting Membrane Protrusion and Polarity William T. Arthur* and Keith Burridge Department of Cell and Developmental Biology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599 Submitted April 20, 2001; Revised May 22, 2001; Accepted June 26, 2001 Monitoring Editor: Mary C. Beckerle The binding of extracellular matrix proteins to integrins triggers rearrangements in the actin cytoskeleton by regulating the Rho family of small GTPases. The signaling events that mediate changes in the activity of Rho proteins in response to the extracellular matrix remain largely unknown. We have demonstrated in previous studies that integrin signaling transiently sup- presses RhoA activity through stimulation of p190RhoGAP. Here, we investigated the biological significance of adhesion-dependent RhoA inactivation by manipulating p190RhoGAP signaling in Rat1 fibroblasts. The inhibition of RhoA activity that is induced transiently by adhesion was antagonized by expression of dominant negative p190RhoGAP. This resulted in impaired cell spreading on a fibronectin substrate, reduced cell protrusion, and premature assembly of stress fibers. Conversely, overexpression of p190RhoGAP augmented cell spreading. Dominant negative p190RhoGAP elevated RhoA activity in cells on fibronectin and inhibited migration, whereas overexpression of the wild-type GAP decreased RhoA activity, promoted the formation of membrane protrusions, and enhanced motility. Cells expressing dominant negative p190RhoGAP, but not control cells or cells overexpressing the wild-type GAP, were unable to establish polarity in the direction of migration. Taken together, these data demonstrate that integrin-triggered RhoA inhibition by p190RhoGAP enhances spreading and migration by regulating cell protrusion and polarity.
    [Show full text]
  • Translocation of Cortactin to the Cell Periphery Is Mediated by the Small Gtpase Rac1
    Journal of Cell Science 111, 2433-2443 (1998) 2433 Printed in Great Britain © The Company of Biologists Limited 1998 JCS4584 Translocation of cortactin to the cell periphery is mediated by the small GTPase Rac1 Scott A. Weed, Yunrui Du and J. Thomas Parsons* Department of Microbiology, Health Sciences Center, University of Virginia, Charlottesville, Virginia 22908, USA *Author for correspondence (E-mail: [email protected]) Accepted 15 June; published on WWW 30 July 1998 SUMMARY Small GTPases of the Rho family regulate signaling translocation of cortactin from cytoplasmic pools into pathways that control actin cytoskeletal structures. In peripheral membrane ruffles. Expression of mutant forms Swiss 3T3 cells, RhoA activation leads to stress fiber and of the serine/threonine kinase PAK1, a downstream effector focal adhesion formation, Rac1 to lamellipoda and of Rac1 and Cdc42 recently demonstrated to trigger membrane ruffles, and Cdc42 to microspikes and filopodia. cortical actin polymerization and membrane ruffling, also Several downstream molecules mediating these effects have led to the translocation of cortactin to the cell cortex, been recently identified. In this report we provide evidence although this was effectively blocked by coexpression of that the intracellular localization of the actin binding Rac1N17. Collectively these data provide evidence for protein cortactin, a Src kinase substrate, is regulated by the cortactin as a putative target of Rac1-induced signal activation of Rac1. Cortactin redistributes from the transduction events involved in membrane ruffling and cytoplasm into membrane ruffles as a result of growth lamellipodia formation. factor-induced Rac1 activation, and this translocation is blocked by expression of dominant negative Rac1N17.
    [Show full text]
  • Diacylglycerol Kinase Α Mediates HGF-Induced Rac Activation and Membrane Ruffling by Regulating Atypical PKC and Rhogdi
    Diacylglycerol kinase α mediates HGF-induced Rac activation and membrane ruffling by regulating atypical PKC and RhoGDI Federica Chianalea,1, Elena Raineroa,1, Cristina Cianflonea, Valentina Bettioa, Andrea Pighinia, Paolo E. Porporatoa, Nicoletta Filigheddua, Guido Serinib,c, Fabiola Sinigagliaa, Gianluca Baldanzia, and Andrea Graziania,2 aDepartment of Clinical and Experimental Medicine and Biotecnologie per la Ricerca Medica Applicata, University Amedeo Avogadro of Piemonte Orientale, 28100 Novara, Italy; bDepartment of Oncological Sciences and Division of Molecular Angiogenesis, Istituto per la Ricerca e la Cura del Cancro, Institute for Cancer Research and Treatment, University of Torino School of Medicine, 10060 Candiolo, Italy; and cCenter for Complex Systems in Molecular Biology and Medicine, University of Torino, 10100 Torino, Italy Edited* by Lewis Clayton Cantley, Harvard Medical School, Boston, MA, and approved December 14, 2009 (received for review July 29, 2009) Diacylglycerol kinases (DGKs) convert diacylglycerol (DAG) into β-chimaerin (5). Conversely, by generating PA, DGKs regulate phosphatidic acid (PA), acting as molecular switches between several signaling proteins, including serine kinases, small-GTPase- DAG- and PA-mediated signaling. We previously showed that Src- regulating proteins, and lipid-metabolizing enzymes (reviewed in dependent activation and plasma membrane recruitment of DGKα refs. 6 and 7). Thus, by regulating in a reciprocal manner the level of are required for growth-factor-induced cell migration and ruffling, both DAG and PA lipid second messengers, DGK enzymes may act through the control of Rac small-GTPase activation and plasma as terminators of DAG-mediated signals as well as activators of membrane localization. Herein we unveil a signaling pathway PA-mediated ones.
    [Show full text]
  • R-Ras Regulates B1-Integrin Trafficking Via Effects on Membrane Ruffling
    Conklin et al. BMC Cell Biology 2010, 11:14 http://www.biomedcentral.com/1471-2121/11/14 RESEARCH ARTICLE Open Access R-Ras regulates b1-integrin trafficking via effects on membrane ruffling and endocytosis Matthew W Conklin1, Aude Ada-Nguema1, Maddy Parsons2, Kristin M Riching1, Patricia J Keely1* Abstract Background: Integrin-mediated cell adhesion and spreading is dramatically enhanced by activation of the small GTPase, R-Ras. Moreover, R-Ras localizes to the leading edge of migrating cells, and regulates membrane protrusion. The exact mechanisms by which R-Ras regulates integrin function are not fully known. Nor is much known about the spatiotemporal relationship between these two molecules, an understanding of which may provide insight into R-Ras regulation of integrins. Results: GFP-R-Ras localized to the plasma membrane, most specifically in membrane ruffles, in Cos-7 cells. GFP-R- Ras was endocytosed from these ruffles, and trafficked via multiple pathways, one of which involved large, acidic vesicles that were positive for Rab11. Cells transfected with a dominant negative form of GFP-R-Ras did not form ruffles, had decreased cell spreading, and contained numerous, non-trafficking small vesicles. Conversely, cells transfected with the constitutively active form of GFP-R-Ras contained a greater number of ruffles and large vesicles compared to wild-type transfected cells. Ruffle formation was inhibited by knock-down of endogenous R- Ras with siRNA, suggesting that activated R-Ras is not just a component of, but also an architect of ruffle formation. Importantly, b1-integrin co-localized with endogenous R-Ras in ruffles and endocytosed vesicles.
    [Show full text]