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The Effects of Exogenous ACTH on 5-3B-Hydroxysteroid Dehydrogenase Activity in the Embryonic Avian Adrenal Gland

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The Effects of Exogenous ACTH on 5-3B-Hydroxysteroid Dehydrogenase Activity in the Embryonic Avian Adrenal Gland Loyola University Chicago Loyola eCommons Master's Theses Theses and Dissertations 1968 The Effects of Exogenous ACTH on 5-3b-hydroxysteroid Dehydrogenase Activity in the Embryonic Avian Adrenal Gland Grover Charles Ericson Loyola University Chicago Follow this and additional works at: https://ecommons.luc.edu/luc_theses Part of the Medicine and Health Sciences Commons Recommended Citation Ericson, Grover Charles, "The Effects of Exogenous ACTH on 5-3b-hydroxysteroid Dehydrogenase Activity in the Embryonic Avian Adrenal Gland" (1968). Master's Theses. 2264. https://ecommons.luc.edu/luc_theses/2264 This Thesis is brought to you for free and open access by the Theses and Dissertations at Loyola eCommons. It has been accepted for inclusion in Master's Theses by an authorized administrator of Loyola eCommons. For more information, please contact [email protected]. This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License. Copyright © 1968 Grover Charles Ericson THE EFFECTS OF EXOGENOUS ACTH ON d -JB-HYDROXYSTEROID DEHYDROGENASE ACTIVITY IN THE EMBRYONIC AVIAN ADRENAL GLAND by Grover Charles Ericson A The.is Submitted to the Faculty ot the Graduate School of La.vo1. University in Partial Fulfillment ot the Requirements for the Degree ot Master ot Science February 1968 BIOGRAPHY Grover Charles Ericson was born in Oak Park, D.linois, on February 17. 1941. He •• graduated f'rom the Naperville COIIUlIW1ity High School, Naperville. D.l1nois in June, 19.59. He entered North Central College, Naperville. Illinois, in September, 19.59, and was awarded the Bachelor of Arts degree in June, 1964. While attending North Central College. the author lllBrried a fellow student, the former Miss Barbara Joye Hoed •• In September, 1964, he began his graduate studies in the Department of' Anatomy, Loyola University Strltch School of Medicine, Chicago. llllnois. ii The author wishes to express his sincere appreoiation to his adviaor. Dr. Lincoln V. DOllDll, Protessor ot Anatomy, and Chairman, Department ot Anatomy, Emeritus, tor his approval of the probl•• 1nTalwable help, guidance and encouragement throughout thia investigation. The author would also like to expre.s hi. appreciation to Dr. Lealie A. Ehmert, Asslatant Prote.sor ot Anatany. tor h1a helpful critlciam and sug­ gestions on histochemical problema, to Dr. Robert C. Clawon. A.sistant Pro­ feasor ot Anatomy tor his advice and counael on avian embryology; and to Dr. Lucia Smelte, Senior Technician in the Department of Anatomy tor her help and counce! in preparing the histological material. Last, but not least, I would like to take th1a opportunity to expre.s my profound gratitude to my w1!e, Barbara. tor the aid. understanding and encouragement so generously provided. Th1a study WlS supported in part by USPBS, NIH, Reaearch Grants AM 03895 and AM 09926, and a USPBS, NIH, General Re.earch Support Grant. The Grants were administered by' Dr. Lincoln V. Damm. i11 TABLE OF CONTENTS Page INTRODUCTION........................................................ 1 REVI&i OF TliE Lnw.TtJRE............................................. 4 Early Development of the Adrenal Glanda........................ 4 The Adrenal Cortex........................................ 4 The Adrenal Me~....................................... ? Adrenal Steroid Production..................................... 8 H1atochami.tr.y ••••••••••••••••••••••••••••••••••••••••••••••••• 10 Ad renal-P1tu1 ta ry- Axia...... • • • • • • • • • • • • • • • • • •• • • • • • • • • • • • • • • • • 1.3 MATERIALS AND METHODS •••••••• •••••••••••••••••••••••••••••••••••••• 20 OBS.E:R.VATIONS ..................... 41' ............ .. ••••••••••••••••••••• 25 Early Development of the Adrenal Gland ..•...•..•....•.•..•..... 25 Development ot Adrenal~-3B-BSD Activity•••••••••••••••••••••• 28 Histochemical Method tor d-.3B-HSD Uaing DBA as the Substrate.. 28 Histochemical Method tor d -3B-HSD Using Pregnenolone as the Substrat••••••••••••••••••••••••••••••••••••••••••••••••••••••• )0 Effecta of Exogenous ACTH on Adrenal d_.3B-HSD Activity........ )0 Stage 22 Embr.7os •••••••••••••••••••••••••••••••••••••••••• J1 stage 23 ~os •••••••••••••••••••••••••••••••••••••••••• 31 stage 23-24 Embry-o ••••••••••••••••••••••••••••••••••••••••• .32 Stage 24 Embr.yoa •••••••.•••••••••••••••••••••••••••••••••• J2 stage 25 Embry-o ••••••••••••••••••••••••••••••••••••••••••• )3 DISCUSSION •••••••••••••••••••••••••••••••••••••••••••.•••••••.•••••• )4 iv TABLe OF CONTENTS (cont'd) Page SUMMARI AND CONCLUSIONS............................................... 40 LITERATURE CITED ••••••••••••••••••••••••••••••••••••••• ~ •••••••••••••• 42 TABLES •••••••••••••••••••••••••.••••.••.•••.•••••••••••••••••••••••••• 48 PLATES AND FIGURES ••••.••••••••••••••••••.•••.••••••••.••••••••••••••• 57 v LIST OF TABLES Table Page 1 MORPHOLOGICAL AND CHRONOLOGICAL AGES OIt' BRa.ffl .AND ~mrrE LEGHORN EMBRyOS ••••••.•••••••••••••••••••••••••••••••••••••••••• 48 2 INTENSITY OF A.DREHAL FORMAZAN DEPOSITION IN d-JB_HSD PREPkRATIONS.................................................... ;0 J TREAT.MEh~ OF STAGE 22 EMBRyOS •••••••••••••••••••••••••••.••••••• 52 4 TREATMENT OF STAGE 23 E1~OS................................... 5) 5 TREATMENT OF STAGE 23-24 ru~yOS................................ 54 6 TREATMENT OF STAGE 24 ru~yos ••••••••••••••••••••••••••••••••••• 55 ? TREATMENT or STAGE 25 ~~yos................................... 56 LIST OF FIGURES FIGURES Pi.Gli: 1 CROSS SECTION OF it. STAGE 21 BROWN LEGHORN ENBRYO............... 57 2 CROSS SECTION OF it. STAGE 22 BRO~~ LEGHORN EMBRYO............... 57 3 A HIGHER MAGNIFICATION OF THE STA.GE 22 BRCHlN LEGHORN E.t1BRIO SHOWN IN FIGURE TWO............................................ 57 4 CROSS SECTION or A STAGE 23 BROWN LEGHORN EMBRyo............... 57 5 A HIGHER MAGNIFICATION OF THE STAGE 23 ~1i LEGHORN EMBRYO SHOWI II FIGURE FOUR........................................... 57 6 CROSS SECTION OF A STAGE 24 BROWN LEGHCIUf EMBRyO............... .58 7 A HIGHER MAGNIFICATION OF THE STAGE 24 BROWN LEGHORN EMBRYO SHOWN IN FIGURE SIX............................................ 58 8 CROSS SECTION or it. STAGE 25 BRaiN LEGHORN mmRYO............... 58 9 CROSS SECTION OF A STAGE 26 WHITE LEGHORN EMBRYO............... 58 10 CROSS SECTION or A STAGE '2:l BROWN LEGHORN EMBRyO............... .58 11 CROSS SECTION OF A STAGE 28 BROWN Lb13HORN EMBRyo............... 59 12 A HIGHER MAGNIFICATION OF THE ADRENAL OF THE STAGE 28 BROWN LEGHORN EMBRYO JHOWN IN FIGUP~ ELEVEN.......................... 59 13 CROSS SECTION OF THE ADRENAL OF A STAGE 29 WHITE LEGHORN EMBRYO .......................................... or • • • • • • • • • • • • • • • • 59 14 CROSS SECTION or THE ADRENAL or A STAGE 30 WHITE LEGHORN ~\fBRYO. • • • • • • • • • •• • • • • • • • • • • •• • • • • • • • • • • • • • • • • • • • • • • •• •• •• • • • • • 59 1.5 CROSS SEeTIO» OF THE ADRENOCORTICAL CELL MASS OF A STAGE 23 COKTROL BROWH LEGHORN EMBRYO INCUBA.TED WITH DEHIDROEPIAN- DROSTERONE (DBA)............................................... 60 16 CROSS SECTION OF THE ADRENOCORTICAL CELL 11ASS OF J,. STAGE 2,3 ACTH TREATED BRC1NN LEGHORN DmRYO SACRIFICED FIVE HOURS P~"T- INJECTION AND INCUBATED WITH DBA............................... 60 LIST OF FIGURES (oonttd) FIGURES PAGE 17 CROSS SECTION OF THE ADRENOCORTICAL CELL }1ASS OF A STAGE 23- 24 CONTROL BRCWH LEGHORN EMBRYO INCUBATED WITH DBA.............. 60 18 CROSS SECTION OF THE ADRENOCORTICAL CELL MASS OF A STAGE 23- 24 ACTH TREATED BROtlH LEGHORB EMBRYO SACRIFICED THREE HOURS POST.INJECTION AND INCUBATED WITH DI~.••••••••••••••••••••••••••• 60 19 CROSS SECTION OF THE ADRENOCORTICAL CELL MASS OF A STAGE 24 CONTROL BROWN LEGHORN EMBRYO INCUBATED WITH DHA ••••••••••••••••• 60 20 CROSS SECTION OF THE ADRENOCORTICAL CELL MASS OF A STAGE 24 ACTH TREATED BROWN J.EGHORN EMBRYO SACRIFICED FOUR HOURS POST- INJECTION AND INCUBATED WITH DHA •••••••••••••••••••••••••••••••• 60 21 CROSS Sl!.'C1'ION OF THE ADRENOCORTICAL CELL MASS OF A STAGE 24 CONTROL WHITE LEGHORN EMBRYO INCUBATED WITH DNA ••••••••••••••••• 61 22 CROSS SECTION OF THE ADREHOCORTICAL CELL MASS OF A STAGE 25 CONTROL BROWN LEGHORN EMBRYO INCUBATED WITH DRA ••••••••••••••••• 61 23 CROSS SECTION OF THE ADRENOCORTICAL CELL MASS OF A STAGE 25 ACTH TREATED BRCWN LEGHORN EMBRYO SACRIFICED FIVE HOURS P~'T- INJECTION AND INCUBATED WITH DRA ••••• ..........•............... 61 24 CROSS SECTION OF THE ADRENOCORTICAL CELL MASS OF A STAGE 25 ACTH TREATED BR(ltIN LEGHORN EMBRYO SACRIFICED 2.5 HOURS POS'T .. INJECTION AND INCUBATED WITH DBA... • • • • • • • • • • •• • • • •• • • • • • • • • • • • • 61 25 CROSS SECTION OF THE ADRENOCORTICAL CELL MASS OF A STAGE 23 CONTROL BR(7NN LEGHORN .l!.'MBRYO INCUBATED -VIITH PREGNENOLONE. • • • • • • • 62 26 CROSS SECTION OF THE ADRENOCORTICAL CELL MASS OF THE STAGE 23 CONTROL BROWN LEGHORN EMBRYO SHGIN IN FIGURE 2.5 IHCUBATED \rlITH DHA.. • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 62 27 CROSS SECTION OF THE ADRENOCORTICAL CELL MASS OF A STAGE 27 CONTROL BROWN LEGHORN EMBRYO INCUBATED WITH PREGNENOLONE........ 62 28 CROSS SECTION OF THE ADRENOCORTICAL CELL MASS OF THE STAGE 27 CONTROL BRCWN LEGHORN EMBRYO SHOWN IN FIGURE 27 INCUBATED WITH DBA •••••.•••••••••••••••••••••••••••••••• * • • • • • • • • • • • • • • • •• 62 viii ABSTRACT This investigation concerned itself primarily with the development ot adrenal d-JB-hydr0JE;Y8teroid dehydrogenase (d-3B-HSD) aotivity and the effects of exogenous
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