Effects of Disposable PET Plastic Bottle Usage and Waste in America's
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Marine Microorganisms: Evolution and Solution to Pollution Fu L Li1, Wang B1,2
COMMENTARY Marine microorganisms: Evolution and solution to pollution Fu L Li1, Wang B1,2 Li FL, Wang B. Marine microorganisms: Evolution and solution to pollution. J Mar Microbiol. 2018;2(1):4-5. nce ocean nurtured life, now she needs our care. Marine microorganism will be an opportunity to further understand ourselves and to seek for new Ois the host of ocean in all ages. We should learn from them humbly. methods of fighting old infections. Marine microorganism is tightly bond with human during the history of evolution and nowadays’ environment pollution. Along with industrial revolution, our marine ecosystem suffered serious pollutions. Microplastics are tiny plastic particles (<5 mm) (Figure 1B), Although the topic is still in debate, life is probably originated from which poison marine lives. Because these microplastics are very hard to be submarine in hydrothermal vent systems (1). In the journey of evolution, our degraded, it is predicted that there will be more microplastics than fish in biosphere was completely dominated by microbes for a very long time (Figure ocean by the year 2050 (7). Since marine sediments are considered as the sink 1A). Human being evolves with those microorganisms. Consequently, of microplastics and marine microbes are key dwellers of marine sediments, the influences of microorganisms can be found in all aspects of human more attention should be paid on the interactions between microplastics biology. More than 65% of our genes originated with bacteria, archaea, and and marine microbes. Actually, a call for this has been published in 2011 unicellular eukaryotes, including those genes responsible for host-microbe (8). -
Recent Advances in Biocatalysts Engineering for Polyethylene Terephthalate Plastic Waste Green Recycling
Environment International 145 (2020) 106144 Contents lists available at ScienceDirect Environment International journal homepage: www.elsevier.com/locate/envint Review article Recent advances in biocatalysts engineering for polyethylene terephthalate plastic waste green recycling Nadia A. Samak a,b,c,1, Yunpu Jia a,b,1, Moustafa M. Sharshar a,b, Tingzhen Mu a, Maohua Yang a, Sumit Peh a,b, Jianmin Xing a,b,* a CAS Key Laboratory of Green Process and Engineering & State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China b College of Chemical Engineering, University of Chinese Academy of Sciences, 19 A Yuquan Road, Beijing 100049, PR China c Processes Design and Development Department, Egyptian Petroleum Research Institute, Nasr City, 11727 Cairo, Egypt ARTICLE INFO ABSTRACT Handling Editor: Guo-ping Sheng The massive waste of poly(ethylene terephthalate) (PET) that ends up in the landfills and oceans and needs hundreds of years for degradation has attracted global concern. The poor stability and productivity of the Keywords: available PET biocatalysts hinder their industrial applications. Active PET biocatalysts can provide a promising Plastic waste avenue for PET bioconversion and recycling. Therefore, there is an urgent need to develop new strategies that Poly(ethylene terephthalate) could enhance the stability, catalytic activity, solubility, productivity, and re-usability of these PET biocatalysts Recycling under harsh conditions such as high temperatures, pH, and salinity. This has raised great attention in using Biocatalysts ’ Bioengineering bioengineering strategies to improve PET biocatalysts robustness and catalytic behavior. Herein, historical and forecasting data of plastic production and disposal were critically reviewed. -
Kidder-Catalogue.Pdf
SHORT SUMMARY INDEX Balloon Helicopter Kit TRANSPORTATION Pg. 4-13 MECHANISMS Pulleys & Gears Pg. 14 &15 ELECTRICITY Pg. 16-20 ALTERNATIVE ENERGY Pg. 21 & 22 Pg. 50 MakerSpace Stations MAGNETICS Pg. 23 & 24 STRUCTURES Wood Building Materials Pg. 25-27 TOOLS & ACCESSORIES Pg. 28-32 MAKERSPACe Pg. 33-34 Pg. 34 WIGGLE-BOTS SAFETY Pg. 35 KIDDERAMA DOLLAR STORE! Pg. 36-38 HYDRAULICS/PNEUMATICS Pg. 39 & 40 TEACHERGEEK Pg. 41 & 42 Pg. 41 FLIGHT/AEROSPACE Pg. 43-45 SAFETY EARTH & SPACE Pg. 46 OPTICS Pg. 47 LABWARE & CHEMISTRY Pg. 48 & 49 Pg. 35 PHYSICS Forces, Mass & Density Pg. 50 TELESCOPES PLASTICS & MANUFACTURING Pg. 51-55 RESOURCE MATERIAL Pg. 56-58 CLOCKS & CRAFT SUPPLIES Pg. 59-69 Pg. 46 A...Bass Body Blanks N CO2 Dragster Supplies Th e basswood blank has a hole for the cartridge cut out. O I It is 30cm x 4cm x 7cm. T CAT# 19-1013 EA ........................QTY 1 $5.60, 2-5 $5.00, 20+ $4.30 A CAT# 19-1014 EA (BALSA)............QTY 1 $7.70 2-5 $6.85 20+ $5.95 T R B...Rear Slicks O Th ese plastic slicks are 40mm in diameter and 16mm thick. 12” A P CAT# 19-1016 EA ................................QTY 1 56¢, 2-5 46, 6-19 42¢ S CAT# 19-54-1016 100/PKG ......................................................$30.00 Also available in BALSA Cat# 19-1014 N CAT# 19-5426 500/PKG ..........................................................$146.50 A R C...Front Wheels T TRANSPORTATION Th ese plastic wheels are 33mm in diameter and 5mm thick. CAT# 19-1015 EA .................................QTY 1 47¢, 2-5 43¢, 6-19 39¢ CAT# 19-54-1015 100/PKG .....................................................$22.50 CAT# 19-5425 500/PK. -
Microbes on a Bottle: Substrate, Season and Geography Influence Community Composition of Microbes Colonizing Marine Plastic Debris
RESEARCH ARTICLE Microbes on a Bottle: Substrate, Season and Geography Influence Community Composition of Microbes Colonizing Marine Plastic Debris Sonja Oberbeckmann1,2,3☯, A. Mark Osborn1,2,4, Melissa B. Duhaime5☯* 1 Department of Biological Sciences, University of Hull, Cottingham Road, Hull HU6 7RX, United Kingdom, a11111 2 School of Life Sciences, University of Lincoln, Brayford Pool Lincoln LN6 7TS, United Kingdom, 3 Environmental Microbiology Working Group, Leibniz Institute for Baltic Sea Research, Warnemünde, Germany, 4 School of Applied Sciences, Royal Melbourne Institute of Technology University, PO Box 77, Bundoora, VIC3083, Australia, 5 Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan, United States of America ☯ These authors contributed equally to this work. * [email protected] OPEN ACCESS Citation: Oberbeckmann S, Osborn AM, Duhaime MB (2016) Microbes on a Bottle: Substrate, Season Abstract and Geography Influence Community Composition of Microbes Colonizing Marine Plastic Debris. PLoS Plastic debris pervades in our oceans and freshwater systems and the potential ecosystem- ONE 11(8): e0159289. doi:10.1371/journal. level impacts of this anthropogenic litter require urgent evaluation. Microbes readily colonize pone.0159289 aquatic plastic debris and members of these biofilm communities are speculated to include Editor: Dee A. Carter, University of Sydney, pathogenic, toxic, invasive or plastic degrading-species. The influence of plastic-colonizing AUSTRALIA microorganisms on the fate of plastic debris is largely unknown, as is the role of plastic in Received: May 11, 2015 selecting for unique microbial communities. This work aimed to characterize microbial bio- Accepted: April 26, 2016 film communities colonizing single-use poly(ethylene terephthalate) (PET) drinking bottles, determine their plastic-specificity in contrast with seawater and glass-colonizing communi- Published: August 3, 2016 ties, and identify seasonal and geographical influences on the communities. -
Structure of the Plastic-Degrading Ideonella Sakaiensis Mhetase Bound to a Substrate
ARTICLE https://doi.org/10.1038/s41467-019-09326-3 OPEN Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate Gottfried J. Palm 1, Lukas Reisky 2, Dominique Böttcher2, Henrik Müller2, Emil A.P. Michels1, Miriam C. Walczak2, Leona Berndt1, Manfred S. Weiss3, Uwe T. Bornscheuer 2 & Gert Weber 1,4 The extreme durability of polyethylene terephthalate (PET) debris has rendered it a long-term environmental burden. At the same time, current recycling efforts still lack sustainability. Two 1234567890():,; recently discovered bacterial enzymes that specifically degrade PET represent a promising solution. First, Ideonella sakaiensis PETase, a structurally well-characterized consensus α/β-hydrolase fold enzyme, converts PET to mono-(2-hydroxyethyl) terephthalate (MHET). MHETase, the second key enzyme, hydrolyzes MHET to the PET educts terephthalate and ethylene glycol. Here, we report the crystal structures of active ligand-free MHETase and MHETase bound to a nonhydrolyzable MHET analog. MHETase, which is reminiscent of feruloyl esterases, possesses a classic α/β-hydrolase domain and a lid domain conferring substrate specificity. In the light of structure-based mapping of the active site, activity assays, mutagenesis studies and a first structure-guided alteration of substrate specificity towards bis-(2-hydroxyethyl) terephthalate (BHET) reported here, we anticipate MHETase to be a valuable resource to further advance enzymatic plastic degradation. 1 Molecular Structural Biology, University of Greifswald, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany. 2 Biotechnology & Enzyme Catalysis, University of Greifswald, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany. 3 Macromolecular Crystallography, Helmholtz-Zentrum Berlin für Materialien und Energie, Albert-Einstein-Straße15, 12489 Berlin, Germany. -
Safe & Unsafe Materials for the Laser Cutter
Safe & Unsafe Materials for the Laser Cutter BARNEYARTLABS.COM Any questions please email [email protected] UNSAFE (NEVER CUT THESE MATERIALS) WARNING: Because many plastics are dangerous to cut, it is important to know what kind you are planning to use. Material DANGER! Cause/Consequence PVC (Poly Vinyl Emits pure chlorine gas when Don't ever cut this material as it Chloride)/vinyl/pleather/artificia cut! will ruin the optics, cause the l leather metal of the machine to corrode, and ruin the motion control system. Thick ( >1mm ) Cut very poorly, discolor, catch Polycarbonate is often found as Polycarbonate/Lexan fire flat, sheet material. The window of the laser cutter is made of Polycarbonate because polycarbonate strongly absorbs infrared radiation! This is the frequency of light the laser cutter uses to cut materials, so it is very ineffective at cutting polycarbonate. Polycarbonate is a poor choice for laser cutting. ABS Emits cyanide gas and tends to ABS does not cut well in a laser melt cutter. It tends to melt rather than vaporize, and has a higher chance of catching on fire and leaving behind melted gooey deposits on the vector cutting grid. It also does not engrave well (again, tends to melt). HDPE/milk bottle plastic Catches fire and melts It melts. It gets gooey. Don't use it. PolyStyrene Foam Catches fire It catches fire, it melts, and only thin pieces cut. This is the #1 material that causes laser fires!!! PolyPropylene Foam Catches fire Like PolyStyrene, it melts, catches fire, and the melted drops continue to burn and turn into rock-hard drips and pebbles. -
A Bacterium That Degrades and Assimilates Poly(Ethylene Terephthalate)
1 Title: A bacterium that degrades and assimilates poly(ethylene terephthalate) 2 † 3 Authors: Shosuke Yoshida1,2 , Kazumi Hiraga1, Toshihiko Takehana3, Ikuo Taniguchi4, 4 Hironao Yamaji1, Yasuhito Maeda5, Kiyotsuna Toyohara5, Kenji Miyamoto2*, Yoshiharu 5 Kimura4, & Kohei Oda1* 6 Affiliations: 7 1Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, 8 Matsugasaki, Sakyo-ku, Kyoto .0.-0 0 , 1apan 9 2Department of Biosciences and Informatics, Keio 2ni3ersity, 3-14-1 Hiyoshi, Kohoku-ku, 10 Yokohama, Kanagawa 223-0 22, 1apan 11 35ife Science Materials 5aboratory, ADEKA Corporation, 7-2-34 Higashiogu, Arakawa-ku, 12 Tokyo 11.-0 3, 1apan 13 4Department of Polymer Science, Faculty of Te,tile Science, Kyoto Institute of Technology, 14 Matsugasaki, Sakyo-ku, Kyoto .0.-0 0 , 1apan 15 Ecology-Related Material Group Inno3ation Research Institute, Teijin 5td., Hinode-cho 2-1, 16 Iwakuni, Yamaguchi 740-0 11, 1apan 17 Current address: Department of Polymer Chemistry, Graduate School of Engineering, Kyoto 18 2ni3ersity, Nishikyo-ku, Kyoto .1 -0 30, 1apan 19 "Correspondence to: K.M. (kmiyamotoAbio.keio.ac.jp) or K.O. (bikaAkit.ac.jp). 20 21 1 22 Abstract: Poly(ethylene terephthalate) (PET) is used e,tensi3ely worldwide in plastic products, 23 and its accumulation in the en3ironment has become a global concern. Because the ability to 24 enzymatically degrade PET for microbial growth has been limited to a few fungal species, 25 biodegradation is not yet a 3iable remediation or recycling strategy. By screening natural 26 microbial communities e,posed to PET in the en3ironment, we isolated a no3el bacterium, 27 Ideonella sakaiensis 201-F., that is able to utilize PET as its major energy and carbon source. -
Enzymatic PET Degradation
GREEN AND SUSTAINABLE CHEMISTRY CHIMIA 2019, 73, No. 9 743 doi:10.2533/chimia.2019.743 Chimia 73 (2019) 743–749 © Swiss Chemical Society Enzymatic PET Degradation Athena Papadopoulou§, Katrin Hecht§, and Rebecca Buller* Abstract: Plastic, in the form of packaging material, disposables, clothing and other articles with a short lifespan, has become an indispensable part of our everyday life. The increased production and use of plastic, however, accelerates the accumulation of plastic waste and poses an increasing burden on the environment with negative effects on biodiversity and human health. PET, a common thermoplastic, is recycled in many countries via ther- mal, mechanical and chemical means. Recently, several enzymes have been identified capable of degrading this recalcitrant plastic, opening possibilities for the biological recycling of the omnipresent material. In this review, we analyze the current knowledge of enzymatic PET degradation and discuss advances in improving the involved enzymes via protein engineering. Looking forward, the use of plastic degrading enzymes may facilitate sustain- able plastic waste management and become an important tool for the realization of a circular plastic economy. Keywords: Biocatalysis · Biodegradation · Enzyme engineering · Plastic recycling · PET Dr. Athena Papadopoulou studied Biology 1. Introduction and received her BSc from the University Plastic has become an omnipresent material in our daily life of Ioannina. She completed her MSc in and, as a consequence, the plastic industry has become the seventh Chemistry with a focus on Chemical and most important industry in Europe, employing more than 1.5 mil- Biochemical Technologies. In 2013 she lion people with a turnover of 355 billion Euros in 2017.[1] Plastic moved to Biotechnology group at the production is cheap and the generated plastic items are durable University of Ioannina to pursue her PhD and versatile. -
Bacteria – Friend Or Foe?
Bacteria – Friend or Foe? By Rachel L. Dittmar & Rosa M. Santana Carrero [email protected], [email protected] April 2020 Danger! Sick! Gross! Vaccine! Disease! Medicine! Dirty! MRSA! Eww! E. coli! What is the first thing that comes to mind when you hear “bacteria”? Science! Microscope! MRSA! Salmonella! Hospital! Germs! Staph! Food! Small! 2 Introduction to Bacteria 3 Bacterial nomenclature • Bacteria are referred to by their genus and species, with genus coming first and species coming last: Escherichia coli Escherichia: genus Species: coli Bacteria names are ALWAYS italicized. Genus names are capitalized and species names are not. Sometimes, the genus is abbreviated by its first initial: E. coli 4 Bacteria are prokaryotes. 5 What are prokaryotes? • Plasma membrane separates the cell from its surrounding environment • Cytoplasm contains organelles • Contain DNA consisting of a single large, circular chromosome • Ribosomes make proteins 6 Prokaryotes v. Eukaryotes 7 Image from: https://www.difference.wiki/prokaryotic-cell-vs-eukaryotic-cell/ How do these organisms differ? Prokaryotes Eukaryotes - circular DNA - linear DNA (found in nucleus) - no nucleus - nucleus - no membrane bound organelles - membrane bound organelles - small- less than 10μm - larger than 10μm - unicellular - can be unicellular and multicellular 8 Bacteria are very small. 9 How big are bacteria? Bacteria are very small: 0.1 – 5.0 micrometers. A micrometer (μm) is 0.000001 meters or 0.001 millimeters (mm) For comparison, a human hair is 30 – 100 μm Image from: https://www.khanacademy.org/science/high-school-biology/hs-cells/hs-prokaryotes-and-eukaryotes/a/prokaryotic-cells 10 Bacteria are classified by phenotype or genotype. -
Flexseal-Couplings-Brochure.Pdf
Flexible Couplings Pioneering | Evolving | Connecting flexseal.co.uk Contents Icon Coupling .........................................................6 Chemical Couplings ........................................ 24 150-400mm + diameter 100-1000mm diameter For the secure internal connection of two pipes Provide a watertight connection where a of irregular or inaccessible external profile chemically inert flexible coupling is required Standard Couplings ...........................................8 Bushes ........................................................................ 26 50-620mm diameter 11-3000+ mm diameter Designed for sewerage, drainage and all non Designed for use with couplings when connecting and low pressure applications up to 2.5 bar pipes of significantly different outside diameters Large Diameter Couplings ......................... 10 Multibush ................................................................ 28 601-2100mm diameter 100, 150 & 200mm diameter Designed for sewerage, drainage and all non and For the connection of DN100, DN150 & DN200 low pressure applications. Manufactured to order pipes of differing outside diameters to suit the outside diameter of any pipe EPDM Plumbing Range ................................ 30 Extra Wide Couplings ..................................... 12 30-118mm diameter 200-3000+ mm diameter Flexible couplings for low pressure Designed for connecting large diameter plumbing applications pipes of any material EPDM End Cap ..................................................... 33 Magnum .................................................................. -
Solar2017-0013-Tandl
Conference Proceedings ASES National Solar Conference 2017 Denver, Colorado 9-12 October 2017 Thermal Collection and Heating Device for Spas John J Tandler Aztech Energy, LLC, Arvada, Colorado, USA Abstract A new type of solar appliance is presented which provides solar energy to heat portable electric spas. The design constraints are such that conventional photovoltaic and solar thermal solutions are not feasible, so a plastic solar collector and radiator is presented with air as the working fluid. An hourly simulation model is presented and validated with field data which shows the device reducing electric energy consumption for heating 43 to 98 percent depending on the climate zone, with simple economic paybacks between 1.5 and 3.5 years. Keywords: solar thermal, solar hot water, polymer solar collector, spa heater, hot tub heater 1. Introduction The portable electric spa is an industry term for a self-standing, electrically-powered hot tub. The purpose of such applicances is to provide thermal comfort and therapeutic treatments by immersion of most of the body in water that is heated to 35-39 degrees C. The majority of such spas are located outdoors, and as such the energy consumption to maintain the desired water temperature consumes a signficiant amount of electricity. Nationwide typical energy consumption for an outdoor electric spa is 800-2000 Wh/yr at a cost of US$200-400/yr. There are over 5.5 million such spas in operation in the United States, and over two million more in Canada and Europe, with about 185,000 new units sold each year in the US[1]. -
Microbes on a Bottle: Substrate, Season and Geography Influence Community Composition of Microbes Colonizing Marine Plastic Debris
RESEARCH ARTICLE Microbes on a Bottle: Substrate, Season and Geography Influence Community Composition of Microbes Colonizing Marine Plastic Debris Sonja Oberbeckmann1,2,3☯, A. Mark Osborn1,2,4, Melissa B. Duhaime5☯* 1 Department of Biological Sciences, University of Hull, Cottingham Road, Hull HU6 7RX, United Kingdom, a11111 2 School of Life Sciences, University of Lincoln, Brayford Pool Lincoln LN6 7TS, United Kingdom, 3 Environmental Microbiology Working Group, Leibniz Institute for Baltic Sea Research, Warnemünde, Germany, 4 School of Applied Sciences, Royal Melbourne Institute of Technology University, PO Box 77, Bundoora, VIC3083, Australia, 5 Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan, United States of America ☯ These authors contributed equally to this work. * [email protected] OPEN ACCESS Citation: Oberbeckmann S, Osborn AM, Duhaime MB (2016) Microbes on a Bottle: Substrate, Season Abstract and Geography Influence Community Composition of Microbes Colonizing Marine Plastic Debris. PLoS Plastic debris pervades in our oceans and freshwater systems and the potential ecosystem- ONE 11(8): e0159289. doi:10.1371/journal. level impacts of this anthropogenic litter require urgent evaluation. Microbes readily colonize pone.0159289 aquatic plastic debris and members of these biofilm communities are speculated to include Editor: Dee A. Carter, University of Sydney, pathogenic, toxic, invasive or plastic degrading-species. The influence of plastic-colonizing AUSTRALIA microorganisms on the fate of plastic debris is largely unknown, as is the role of plastic in Received: May 11, 2015 selecting for unique microbial communities. This work aimed to characterize microbial bio- Accepted: April 26, 2016 film communities colonizing single-use poly(ethylene terephthalate) (PET) drinking bottles, determine their plastic-specificity in contrast with seawater and glass-colonizing communi- Published: August 3, 2016 ties, and identify seasonal and geographical influences on the communities.