(COVIS) Overview
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Genomics 98 (2011) 370–375
Genomics 98 (2011) 370–375 Contents lists available at ScienceDirect Genomics journal homepage: www.elsevier.com/locate/ygeno Whole-genome comparison clarifies close phylogenetic relationships between the phyla Dictyoglomi and Thermotogae Hiromi Nishida a,⁎, Teruhiko Beppu b, Kenji Ueda b a Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan b Life Science Research Center, College of Bioresource Sciences, Nihon University, Fujisawa, Japan article info abstract Article history: The anaerobic thermophilic bacterial genus Dictyoglomus is characterized by the ability to produce useful Received 2 June 2011 enzymes such as amylase, mannanase, and xylanase. Despite the significance, the phylogenetic position of Accepted 1 August 2011 Dictyoglomus has not yet been clarified, since it exhibits ambiguous phylogenetic positions in a single gene Available online 7 August 2011 sequence comparison-based analysis. The number of substitutions at the diverging point of Dictyoglomus is insufficient to show the relationships in a single gene comparison-based analysis. Hence, we studied its Keywords: evolutionary trait based on whole-genome comparison. Both gene content and orthologous protein sequence Whole-genome comparison Dictyoglomus comparisons indicated that Dictyoglomus is most closely related to the phylum Thermotogae and it forms a Bacterial systematics monophyletic group with Coprothermobacter proteolyticus (a constituent of the phylum Firmicutes) and Coprothermobacter proteolyticus Thermotogae. Our findings indicate that C. proteolyticus does not belong to the phylum Firmicutes and that the Thermotogae phylum Dictyoglomi is not closely related to either the phylum Firmicutes or Synergistetes but to the phylum Thermotogae. © 2011 Elsevier Inc. -
Vibrio Species in an Urban Tropical Estuary: Antimicrobial Susceptibility, Interaction with Environmental Parameters, and Possible Public Health Outcomes
microorganisms Article Vibrio Species in an Urban Tropical Estuary: Antimicrobial Susceptibility, Interaction with Environmental Parameters, and Possible Public Health Outcomes Anna L. B. Canellas 1 , Isabelle R. Lopes 1 , Marianne P. Mello 2, Rodolfo Paranhos 2, Bruno F. R. de Oliveira 1 and Marinella S. Laport 1,* 1 Laboratório de Bacteriologia Molecular e Marinha, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941902, Brazil; [email protected] (A.L.B.C.); [email protected] (I.R.L.); [email protected] (B.F.R.d.O.) 2 Departamento de Biologia Marinha, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941617, Brazil; [email protected] (M.P.M.); [email protected] (R.P.) * Correspondence: [email protected] Abstract: The genus Vibrio comprises pathogens ubiquitous to marine environments. This study evaluated the cultivable Vibrio community in the Guanabara Bay (GB), a recreational, yet heavily polluted estuary in Rio de Janeiro, Brazil. Over one year, 66 water samples from three locations along a pollution gradient were investigated. Isolates were identified by MALDI-TOF mass spectrometry, revealing 20 Vibrio species, including several potential pathogens. Antimicrobial susceptibility testing confirmed resistance to aminoglycosides, beta-lactams (including carbapenems and third-generation cephalosporins), fluoroquinolones, sulfonamides, and tetracyclines. Four strains were producers Citation: Canellas, A.L.B.; Lopes, of extended-spectrum beta-lactamases (ESBL), all of which carried beta-lactam and heavy metal I.R.; Mello, M.P.; Paranhos, R.; de resistance genes. The toxR gene was detected in all V. parahaemolyticus strains, although none carried Oliveira, B.F.R.; Laport, M.S. -
Genomic Signatures of Predatory Bacteria
The ISME Journal (2013) 7, 756–769 & 2013 International Society for Microbial Ecology All rights reserved 1751-7362/13 www.nature.com/ismej ORIGINAL ARTICLE By their genes ye shall know them: genomic signatures of predatory bacteria Zohar Pasternak1, Shmuel Pietrokovski2, Or Rotem1, Uri Gophna3, Mor N Lurie-Weinberger3 and Edouard Jurkevitch1 1Department of Plant Pathology and Microbiology, The Hebrew University of Jerusalem, Rehovot, Israel; 2Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel and 3Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel Predatory bacteria are taxonomically disparate, exhibit diverse predatory strategies and are widely distributed in varied environments. To date, their predatory phenotypes cannot be discerned in genome sequence data thereby limiting our understanding of bacterial predation, and of its impact in nature. Here, we define the ‘predatome,’ that is, sets of protein families that reflect the phenotypes of predatory bacteria. The proteomes of all sequenced 11 predatory bacteria, including two de novo sequenced genomes, and 19 non-predatory bacteria from across the phylogenetic and ecological landscapes were compared. Protein families discriminating between the two groups were identified and quantified, demonstrating that differences in the proteomes of predatory and non-predatory bacteria are large and significant. This analysis allows predictions to be made, as we show by confirming from genome data an over-looked bacterial predator. The predatome exhibits deficiencies in riboflavin and amino acids biosynthesis, suggesting that predators obtain them from their prey. In contrast, these genomes are highly enriched in adhesins, proteases and particular metabolic proteins, used for binding to, processing and consuming prey, respectively. -
Interactions Between the Human Pathogen Vibrio Parahaemolyticus and Common Marine Microalgae
Current Trends in Microbiology Vol. 12, 2018 Interactions between the human pathogen Vibrio parahaemolyticus and common marine microalgae Savannah L. Klein, Katherine E. Haney, Thomas M. Hornaday, India B. Gartmon and Charles R. Lovell* Department of Biological Sciences, University of South Carolina, Columbia, South Carolina, 29208, USA. ABSTRACT KEYWORDS: Vibrio parahaemolyticus, tdh, trh, T3SS2, T6SS, microalgae. Vibrio parahaemolyticus is a gastrointestinal pathogen that is abundant in coastal marine environments. Elevated numbers of V. parahaemolyticus cells INTRODUCTION have been correlated with marine microalgae Vibrio parahaemolyticus, a common organism in blooms, particularly blooms of diatoms and coastal environments, is a significant and sometimes dinoflagellates, but the nature of the relationship pandemic human pathogen responsible for an between V. parahaemolyticus and microalgae is estimated 34,000 cases of seafood-associated unknown. We performed in vitro assays using 27 gastroenteritis per year in the United States [1]. Most environmental V. parahaemolyticus strains and cases of V. parahaemolyticus-induced gastroenteritis various phototrophs; a diatom, a dinoflagellate, are self-limiting and relatively mild, but infections unarmored and armored forms of a coccolithophore, can be deadly in immunocompromised individuals. and two species of cyanobacteria. The V. The common mode of transmission of this parahaemolyticus strains we employed contained bacterium to the human host is ingestion of raw or different combinations of virulence-correlated genes, undercooked shellfish, primarily oysters. In addition, the hemolysin genes tdh and trh, the Type III some strains of V. parahaemolyticus can infect Secretion System 2 (T3SS2) marker gene vscC2, wounds and some produce systemic infections, and the Type VI Secretion System (T6SS) marker while others are apparently non-pathogenic. -
BACTERIAL and PHAGE INTERACTIONS INFLUENCING Vibrio Parahaemolyticus ECOLOGY
University of New Hampshire University of New Hampshire Scholars' Repository Master's Theses and Capstones Student Scholarship Spring 2016 BACTERIAL AND PHAGE INTERACTIONS INFLUENCING Vibrio parahaemolyticus ECOLOGY Ashley L. Marcinkiewicz University of New Hampshire, Durham Follow this and additional works at: https://scholars.unh.edu/thesis Recommended Citation Marcinkiewicz, Ashley L., "BACTERIAL AND PHAGE INTERACTIONS INFLUENCING Vibrio parahaemolyticus ECOLOGY" (2016). Master's Theses and Capstones. 852. https://scholars.unh.edu/thesis/852 This Thesis is brought to you for free and open access by the Student Scholarship at University of New Hampshire Scholars' Repository. It has been accepted for inclusion in Master's Theses and Capstones by an authorized administrator of University of New Hampshire Scholars' Repository. For more information, please contact [email protected]. BACTERIAL AND PHAGE INTERACTIONS INFLUENCING Vibrio parahaemolyticus ECOLOGY BY ASHLEY MARCINKIEWICZ Bachelor of Arts, Wells College, 2011 THESIS Submitted to the University of New Hampshire In Partial Fulfillment of The Requirements for the Degree of Master of Science in Microbiology May, 2016 This thesis has been examined and approved in partial fulfillment of the requirements for the degree of Masters of Science in Microbiology by: Thesis Director, Cheryl A. Whistler Associate Professor of Molecular, Cellular, and Biomedical Sciences Stephen H. Jones Research Associate Professor of Natural Resources and the Environment Jeffrey T. Foster Assistant Professor of Molecular, Cellular, and Biomedical Sciences On April 15th, 2016 Original approved signatures are on file with the University of New Hampshire Graduate School. iii TABLE OF CONTENTS ACKNOWLEDGEMENTS………………………………………………………... vi LIST OF TABLES………………………………………………………………… vii LIST OF FIGURES…….………………………………………………………….. viii ABSTRACT………………………………………………………………………. -
Unlocking the Genomic Taxonomy of the Prochlorococcus Collective
bioRxiv preprint doi: https://doi.org/10.1101/2020.03.09.980698; this version posted March 11, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Unlocking the genomic taxonomy of the Prochlorococcus collective Diogo Tschoeke1#, Livia Vidal1#, Mariana Campeão1, Vinícius W. Salazar1, Jean Swings1,2, Fabiano Thompson1*, Cristiane Thompson1* 1Laboratory of Microbiology. SAGE-COPPE and Institute of Biology. Federal University of Rio de Janeiro. Rio de Janeiro. Brazil. Av. Carlos Chagas Fo 373, CEP 21941-902, RJ, Brazil. 2Laboratory of Microbiology, Ghent University, Gent, Belgium. *Corresponding authors: E-mail: [email protected] , [email protected] Phone no.: +5521981041035, +552139386567 #These authors contributed equally. ABSTRACT 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.03.09.980698; this version posted March 11, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Prochlorococcus is the most abundant photosynthetic prokaryote on our planet. The extensive ecological literature on the Prochlorococcus collective (PC) is based on the assumption that it comprises one single genus comprising the species Prochlorococcus marinus, containing itself a collective of ecotypes. Ecologists adopt the distributed genome hypothesis of an open pan-genome to explain the observed genomic diversity and evolution patterns of the ecotypes within PC. -
S41598-020-68891-6.Pdf
www.nature.com/scientificreports OPEN Structure and co‑occurrence patterns of bacterial communities associated with white faeces disease outbreaks in Pacifc white‑leg shrimp Penaeus vannamei aquaculture Yustian Rovi Alfansah1,2,3*, Sonja Peters1, Jens Harder4, Christiane Hassenrück1,7 & Astrid Gärdes1,5,6,7 Bacterial diseases cause production failures in shrimp aquacultures. To understand environmental conditions and bacterial community dynamics contributing to white faeces disease (WFD) events, we analysed water quality and compared bacterial communities in water as well as in intestines and faeces of healthy and diseased shrimps, respectively, via 16S rRNA gene sequencing and qPCR of transmembrane regulatory protein (toxR), thermolabile haemolysin (tlh), and thermostable direct haemolysin genes of pathogenic Vibrio parahaemolyticus as a proxy for virulence. WFD occurred when pH decreased to 7.71–7.84, and Alteromonas, Pseudoalteromonas and Vibrio dominated the aquatic bacterial communities. The disease severity further correlated with increased proportions of Alteromonas, Photobacterium, Pseudoalteromonas and Vibrio in shrimp faeces. These opportunistic pathogenic bacteria constituted up to 60% and 80% of the sequences in samples from the early and advances stages of the disease outbreak, respectively, and exhibited a high degree of co-occurrence. Furthermore, toxR and tlh were detected in water at the disease event only. Notably, bacterial community resilience in water occurred when pH was adjusted to 8. Then WFD ceased without a mortality event. In conclusion, pH was a reliable indicator of the WFD outbreak risk. Dissolved oxygen and compositions of water and intestinal bacteria may also serve as indicators for better prevention of WFD events. Bacterial diseases are a major problem for Penaeus vannamei pond aquaculture in Asia and Latin America. -
Luxr Solos in Photorhabdus Species
ORIGINAL RESEARCH ARTICLE published: 18 November 2014 CELLULAR AND INFECTION MICROBIOLOGY doi: 10.3389/fcimb.2014.00166 LuxR solos in Photorhabdus species Sophie Brameyer 1, Darko Kresovic 2, Helge B. Bode 2* and Ralf Heermann 1* 1 Bereich Mikrobiologie, Biozentrum, Ludwig-Maximilians-Universität München, München, Germany 2 Fachbereich Biowissenschaften, Merck Stiftungsprofessur für Molekulare Biotechnologie, Goethe-Universität Frankfurt, Frankfurt am Main, Germany Edited by: Bacteria communicate via small diffusible molecules to mediate group-coordinated Vittorio Venturi, International Centre behavior, a process designated as quorum sensing. The basic molecular quorum sensing for Genetic Engineering and system of Gram-negative bacteria consists of a LuxI-type autoinducer synthase producing Biotechnology, Italy acyl-homoserine lactones (AHLs) as signaling molecules, and a LuxR-type receptor Reviewed by: Brian Ahmer, The Ohio State detecting the AHLs to control expression of specific genes. However, many proteobacteria University, USA possess one or more unpaired LuxR-type receptors that lack a cognate LuxI-like synthase, Eric Déziel, Institut National de la referred to as LuxR solos. The enteric and insect pathogenic bacteria of the genus Recherche Scientifique, Canada Photorhabdus harbor an extraordinarily high number of LuxR solos, more than any other *Correspondence: known bacteria, and all lack a LuxI-like synthase. Here, we focus on the presence Helge B. Bode, Fachbereich Biowissenschaften, Merck and the different types of LuxR solos in the three known Photorhabdus species Stiftungsprofessur für Molekulare using bioinformatics analyses. Generally, the N-terminal signal-binding domain (SBD) of Biotechnologie, Goethe-Universität LuxR-type receptors sensing AHLs have a motif of six conserved amino acids that is Frankfurt, Max-von-Laue-Str. -
Diverse Deep-Sea Anglerfishes Share a Genetically Reduced Luminous
RESEARCH ARTICLE Diverse deep-sea anglerfishes share a genetically reduced luminous symbiont that is acquired from the environment Lydia J Baker1*, Lindsay L Freed2, Cole G Easson2,3, Jose V Lopez2, Dante´ Fenolio4, Tracey T Sutton2, Spencer V Nyholm5, Tory A Hendry1* 1Department of Microbiology, Cornell University, New York, United States; 2Halmos College of Natural Sciences and Oceanography, Nova Southeastern University, Fort Lauderdale, United States; 3Department of Biology, Middle Tennessee State University, Murfreesboro, United States; 4Center for Conservation and Research, San Antonio Zoo, San Antonio, United States; 5Department of Molecular and Cell Biology, University of Connecticut, Storrs, United States Abstract Deep-sea anglerfishes are relatively abundant and diverse, but their luminescent bacterial symbionts remain enigmatic. The genomes of two symbiont species have qualities common to vertically transmitted, host-dependent bacteria. However, a number of traits suggest that these symbionts may be environmentally acquired. To determine how anglerfish symbionts are transmitted, we analyzed bacteria-host codivergence across six diverse anglerfish genera. Most of the anglerfish species surveyed shared a common species of symbiont. Only one other symbiont species was found, which had a specific relationship with one anglerfish species, Cryptopsaras couesii. Host and symbiont phylogenies lacked congruence, and there was no statistical support for codivergence broadly. We also recovered symbiont-specific gene sequences from water collected near hosts, suggesting environmental persistence of symbionts. Based on these results we conclude that diverse anglerfishes share symbionts that are acquired from the environment, and *For correspondence: that these bacteria have undergone extreme genome reduction although they are not vertically [email protected] (LJB); transmitted. -
The Evolution of Bacterial Shape Complexity by a Curvature-Inducing Module
bioRxiv preprint doi: https://doi.org/10.1101/2020.02.20.954503; this version posted February 20, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Title: The evolution of bacterial shape complexity by a curvature-inducing module Authors: Nicholas R. Martin, Edith Blackman, Benjamin P. Bratton, Thomas M. Bartlett†, Zemer Gitai*. Affiliations: Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA *Correspondence to: [email protected] †Present address: Department of Microbiology, Harvard Medical School, Boston, MA 02115 Abstract: Bacteria can achieve a staggering diversity of cell shapes that promote critical functions like growth, motility, and virulence1-4. Previous studies suggested that bacteria establish complex shapes by co-opting the core machineries essential for elongation and division5,6. In contrast, we discovered a two-protein module, CrvAB, that can curve bacteria autonomously of the major elongation and division machinery by forming a dynamic, asymmetrically-localized structure in the periplasm. CrvAB is essential for curvature in its native species, Vibrio cholerae, and is sufficient to curve multiple heterologous species spanning 2.5 billion years of evolution. Thus, modular shape determinants can promote the evolution of morphological complexity independently of existing cell shape regulation. Main Text: Cells come in a variety of shapes that contribute to fitness in diverse environments 1,2,7,8. In bacteria, cell curvature can influence motility and host colonization 1,3,4. -
Diversity and Evolution of Bacterial Bioluminescence Genes in the Global Ocean Thomas Vannier, Pascal Hingamp, Floriane Turrel, Lisa Tanet, Magali Lescot, Y
Diversity and evolution of bacterial bioluminescence genes in the global ocean Thomas Vannier, Pascal Hingamp, Floriane Turrel, Lisa Tanet, Magali Lescot, Y. Timsit To cite this version: Thomas Vannier, Pascal Hingamp, Floriane Turrel, Lisa Tanet, Magali Lescot, et al.. Diversity and evolution of bacterial bioluminescence genes in the global ocean. NAR Genomics and Bioinformatics, Oxford University Press, 2020, 2 (2), 10.1093/nargab/lqaa018. hal-02514159 HAL Id: hal-02514159 https://hal.archives-ouvertes.fr/hal-02514159 Submitted on 21 Mar 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Published online 14 March 2020 NAR Genomics and Bioinformatics, 2020, Vol. 2, No. 2 1 doi: 10.1093/nargab/lqaa018 Diversity and evolution of bacterial bioluminescence genes in the global ocean Thomas Vannier 1,2,*, Pascal Hingamp1,2, Floriane Turrel1, Lisa Tanet1, Magali Lescot 1,2,* and Youri Timsit 1,2,* 1Aix Marseille Univ, Universite´ de Toulon, CNRS, IRD, MIO UM110, 13288 Marseille, France and 2Research / Federation for the study of Global Ocean Systems Ecology and Evolution, FR2022 Tara GOSEE, 3 rue Michel-Ange, Downloaded from https://academic.oup.com/nargab/article-abstract/2/2/lqaa018/5805306 by guest on 21 March 2020 75016 Paris, France Received October 21, 2019; Revised February 14, 2020; Editorial Decision March 02, 2020; Accepted March 06, 2020 ABSTRACT ganisms and is particularly widespread in marine species (7–9). -
Revival and Phylogenetic Analysis of the Luminous Bacterial Cultures of MW Beijerinck
RESEARCH ARTICLE Historical microbiology: revival and phylogenetic analysis of the luminous bacterial cultures of M. W. Beijerinck Marian J. Figge1, Lesley A. Robertson2, Jennifer C. Ast3 & Paul V. Dunlap3 1The Netherlands Culture Collection of Bacteria, CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands; 2Department of Biotechnology, Delft University of Technology, Delft, The Netherlands; and 3Department of Ecological and Evolutionary Biology, University of Michigan, Ann Arbor, MI, USA Correspondence: Paul V. Dunlap, Abstract Department of Ecology and Evolutionary Biology, University of Michigan, 830 North Luminous bacteria isolated by Martinus W. Beijerinck were sealed in glass University Ave., Ann Arbor, MI 48109-1048, ampoules in 1924 and 1925 and stored under the names Photobacterium phos- USA. Tel.: +1 734 615 9099; fax: +1 734 phoreum and ‘Photobacterium splendidum’. To determine if the stored cultures 763 0544; e-mail: [email protected] were viable and to assess their evolutionary relationship with currently recog- nized bacteria, portions of the ampoule contents were inoculated into culture Present address: Jennifer C. Ast, medium. Growth and luminescence were evident after 13 days of incubation, Evolutionary Biology Centre, Departments of Molecular Evolution and Limnology, Uppsala indicating the presence of viable cells after more than 80 years of storage. The University, Norbyva¨ gen 18C, 752 36, Beijerinck strains are apparently the oldest bacterial cultures to be revived from Uppsala, Sweden. storage. Multi-locus sequence analysis, based on the 16S rRNA, gapA, gyrB, pyrH, recA, luxA, and luxB genes, revealed that the Beijerinck strains are distant Received 3 May 2011; revised 21 July 2011; from the type strains of P.