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Taqman® Human, Mouse, and Rat GPCR Arrays

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TaqMan® Gene Signature Arrays TaqMan® Human, Mouse, GPCR subfamilies included in GPCR Arrays for human, mouse, or rat and Rat GPCR Arrays Acetylcholine (muscarinic) receptors Melanocortins receptors Adenosine and adenine nucleotide Melanotonin receptors receptors Neuromedin U receptors Adrenergic receptors Neuropeptide FF receptors Adrenomedullin receptors Neuropeptide S receptors Angiotensin receptors Neuropeptide Y receptors ® These arrays are part of a collection of TaqMan Gene Apelin receptors Signature Arrays that enable analysis of hundreds of TaqMan® Neuropeptides B/W receptors Bile acid receptors Gene Expression Assays on a micro fluidic card with minimal Neurotensin receptors Bombesin receptors effort. Nicotinic acid receptors Bradykinin receptors G-Protein Coupled Receptors (GPCRs) are a superfamily of Opioid peptide receptors Cannabinoids receptors integral membrane proteins with seven membrane-spanning Opsins helices. GPCRs detect signals such as a peptide, a protein, a Chemokines and chemotactic factors receptors Orexins receptors small molecule, or an ion or a photon that causes a change Orphan receptors in a retinal group. The signal is transduced from the cell surface Cholecystokinin / gastrin receptors through the transmembrane domains to the cytosol where a Cysteinyl leukotriene receptors Other receptors trimeric G-protein becomes activated, and the G-protein trimer Dopamine receptors Platelet-activating factor receptors elicits a response in the cell. Endothelin receptors Prokineticin receptors GPCRs are the targets for about 50% of all existing drugs. Family 2 (B) receptors Prostanoids receptors Well-known medications such as beta blockers, antihistamines Family 3 (C) receptors Proteinase-activated receptors and narcotic analgesics act on GPCRs. Drug indications (metabotropic glutamate and calcium Relaxin receptors include inflammation, asthma, neurologic disorders, thrombosis, receptors) Releasing hormones receptors and hypertension. Family fz/smo receptors Serotonin receptors We have generated distinct TaqMan GPCR Arrays for human, Free fatty acid receptors Somatostatin and urotensin mouse and rat. The GPCR targets selected fall into the 50 Glycoprotein hormones receptors receptors subfamilies listed, many of which are drug targets or disease Histamine receptors Tachykinin receptors associated. We have not included odorant, olfactory, gustatory Kisspeptins receptors Trace amine receptors or pheromone receptors on these arrays. Krebs cycle intermediates receptors Vasopressin / oxytocin receptors References: Lysolipids receptors GPCR classification: http://us.expasy.org/cgi-bin/lists?7tmrlist.txt Melanin-concentrating hormone receptors TaqMan® Gene Signature Arrays Gene Signature Array Name # of Targets/Controls Format Pack Size Part Number Human GPCR Array 367/14 Format 384 4 arrays/pack 4367785 Mouse GPCR Array 365/16 Format 384 4 arrays/pack 4378703 Rat GPCR Array 365/16 Format 384 4 arrays/pack 4378709 More arrays will be available soon! Register to receive new Gene Signature Array product announcements, or suggest an array at taqmanarray.appliedbiosystems.com Human GPCR Array A ADCYAP1R1 ADMR ADORA1 ADORA2AADORA2B ADORA3 ADRA1A ADRA1B ADRA1D ADRA2A 18S 18S ADRA2B ADRB1 ADRB2 ADRB3 AGTR1 AGTR2 AGTRL1 AVPR1A AVPR1B AVPR2 BAI1 BAI2 1 B BAI3 BDKRB1 BDKRB2 BLR1 BRS3 C11ORF4 C3AR1 C5R1 CALCR CALCRL 18S 18S CASR CCBP2 CCKAR CCKBR CCR1 CCR2 CCR3 CCR4 CCR5 CCR6 CCR7 CCR8 C CCR9 CCRL1 CCRL2 CD97 CELSR1 CELSR2 CELSR3 CHRM1 CHRM2 CHRM3 CHRM4 CHRM5 CMKLR1 CNR1 CNR2 CRHR1 CRHR2 CX3CR1 CXCR3 CXCR4 CXCR6 CYSLTR1 CYSLTR2 DRD1 2 D DRD2 DRD3 DRD4 DRD5 EBI2 EDG1 EDG2 EDG3 EDG4 EDG5 EDG6 EDG7 EDG8 EDNRA EDNRB ELTD1 EMR1 EMR2 EMR3 GPR37L1 F2RF2RL1 F2RL2 F2RL3 E GPR174 FKSG83 OR7E5P FPR1 FPRL1 FPRL2 FSHR FY FZD1 FZD10 FZD2 FZD3 FZD4 FZD5 FZD6 FZD7 FZD8 FZD9 GABBR1 GALR1 GALR2 GALR3 GCGR GHRHR 3 F GHSR GIPR GLP1R GLP2R GNRHR GNRHR2 GPBAR1 GPR GPR1 GPR10 GPR101 GPR103 P2RY14 GPR110 GPR111 GPR112 GPR113 GPR114 GPR115 GPR116 GPR12 GPR120 GPR123 GPR124 G GPR125 GPR126 GPR128 GPR133 GPR135 UTS2R GPR141 GPR142 GPR143 GPR145 GPR146 GPR148 GPR15 GPR150 GPR152 GPR153 GPR160 GPR161 GPR17 GPR18 GPR19 CCR10 GPR20 GPR21 4 H GPR22 GPR23 GPR24 GPR25 GPR26 GPR27 GPR3 GPR30 GPR31 GPR32 TRBV5-4 GPR34 GPR35 GPR37 MLNR GPR39 GPR4 GPR40 GPR43 GPR44 GPR45 LGR4 LGR5 GPR50 I GPR51 GPR52 GPR54 GPR55 GPR56 TAAR2 GPR6 GPR61 GPR62 GPR63 GPR64 GPR65 NMUR1 GPR68 GPR7 GPR73 GPR73L1 GPR74 GPR75 GPR77 GPR78 GPR8 OXGR1 GPR81 5 J GPR82 GPR83 GPR84 GPR85 P2RY13 GPR87 GPR88 SUCNR1 GPR92 GPR97 GPRC5B GPRC5C GPRC5D GPRC6A GRM1 GRM2 GRM3 GRM4 GRM5 GRM6 GRM7 GRM8 GRPR GPR171 K HCRTR1 HCRTR2 HDAC3 HRH1 HRH2 HRH3 HRH4 HTR1A HTR1B HTR1D HTR1E HTR1F HTR2A HTR2B HTR2C HTR4 HTR5A HTR6 HTR7 IL8RA IL8RBLANCL1 LANCL2 LGR6 6 L LGR7 LHCGR SLC26A7 LPHN1 LPHN2 LPHN3 LTB4R LTB4R2 MAS1 MAS1L MASS1 MATK MC1R MC2R MC3R MC4R MC5R MRGPRF MRGPRD MRGPRX1 MRGPRX2 MRGPRX3 MRGPRX4 MTNR1A M MTNR1B NMBR NMUR2 NPY1R NPY2R NPY5R NPY6R NTSR1 NTSR2 RARS GCNT2 OPN1SW OPN3 OPN4 OPRD1 OPRK1 OPRL1 OPRM1 SENP3 OR2A4 OR2C3 PTPN22 MELL1 PHGDH 7 N OR7C2 GPR147 OXTR P2RY1 P2RY10 P2RY11 P2RY12 P2RY2 P2RY4 P2RY5 P2RY6 P2RY8 PCDH15 TAAR5 PPYR1 PTAFR PTGDR PTGER1 PTGER2 PTGER3 PTGER4 PTGFR PTGIR PTHR1 O PTHR2 GPRC5A RGR RHO RRH RLN3R1 SCTR SMO GPR173 SSTR1 SSTR2 SSTR3 SSTR4 SSTR5 TAAR6 TACR1 TACR2 TACR3 TAAR1 TAAR9 TAS1R1 LONPL TBXA2R OXER1 8 P TM7SF1 TAAR8 TRHR TSHR VIPR1 VIPR2 VN1R1 VN1R2 VN1R5 XCR1 MRGPRE GAPDH RPLP0 ACTB PPIA PGK1 B2M GUSB HPRT1 TBP TFRC HMBS IPO8 POLR2A 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Port Mouse GPCR Array A Adra1b Adra2c Adrb1 Bdkrb1 Blr1 Calcr Cckbr Chrm1 Chrm4 Cnr1 18S 18S Crhr1 Drd3 Drd4 Ednrb F2rl1 F2rl3 Fzd4 Fzd8 Galr1 Gcgr Grm8 Hrh1 1 B Hrh2 Htr1d Htr2b Htr2c Htr4 Htr5a Htr7 Mas1 Mc1r Mc2r 18S 18S Mc3r Mtnr1a Ntsr2 Ppyr1 Ptgdr Ptger2 Ptger4 Ptgfr Sstr1 Sstr3 Sstr4 Tacr1 C Tacr2 Vipr2 Bdkrb2 Cckar Ccr1 Crhr2 Gpr44 Drd2 F2r F2rl2 Lgr5 Gpr83 Gnrhr Edg2 Gpr50 Htr1b Htr5b Npy6r Gpr143 Oprl1 Pthr1 Frzb Tshr Agtrl1 2 D Xcr1 Adra1a Adrb3 Fpr1 Fshr Lhcgr Mc5r Oprd1 Trhr Casr Galr3 Avpr1a Ntsr1 Gabbr1 P2ry4 Glp1r Htr6 Tacr3 Fzd1 Fzd3 Fzd5 Gpr63 Chrm3 Gpr45 E Lgr8 Hrh3 Vipr1 Mc4r Celsr2 Gprc5b Emr4 Gpr85 Gpr156 Celsr1 Celsr3 Mtnr1b Gprc6a Edg6 Edg4 Edg7 Taar1 Kiss1r Gpr124 Mass1 Adra2b Fpr-rs2 Fpr-rs3 Fpr-rs4 3 F Sfrp2 Sfrp1 Ramp1 Ramp2 Gpr162 Ltb4r2 Gpr97 Npffr2 C5ar1 Leng4 Ccr3 Nmur1 Cd97 Ccrl2 Calcrl Prokr1 Gpr87 Gprc5c Lgr4 Htr2a Gpr128 Bai2 Gpbar1 Bai1 G Fzd10 Gpr61 Gpr75 Gpr68 Gpr81 Gpr4 Gpr82 Gpr154 Glp2r Nmur2 P2ry5 Ghsr Gpr123 Npy1r Gpr137b Mchr1 Mrgpra4 Bai3 Opn5 Gpr108 Gpr64 Gprc5a Gpr120 Gpr142 4 H Grm3 Galr2 Il8ra Gpr119 Gpr135 Gpr141 Rxfp4 Prokr2 Fpr-rs6 Ptgir Adora2a Adora3 Gpr149 Ffar1 Gpr56 Fpr-rs7 Adora2b Dusp2 Ramp3 Grm6 Adra2a Ccr2 Drd5 Fzd7 I Gpr151 Gpr3 Htr1a P2ry1 Pthr2 ACTB B2M GAPDH GUSB HMBS HPRT1 IPO8 PGK1 POLR2A PPIA RPLP2 TBP TFRC UBC YWHAZ Adcyap1r1 Fzd6 Sstr2 Tbxa2r 5 J Ptger1 Hrh4 Eltd1 Rxfp3 Grm5 Emr1 Gpr153 Grm1 Smo Gpr26 Npffr1 Hcrtr2 Gpr175 Hcrtr1 Oprm1 Grm7 Avpr2 Crcp Gpr133 Gpr125 Lphn3 Lgr7 Gpr113 Oprk1 K Gpr137 Grm2 Ednra Lphn1 Gpr114 Tm7sf3 Gprc5d Prlhr Gpr77 Gpr116 Gpr176 Gpr115 Gpr139 Gpr111 Sctr Gpr161 Lgr6 Gpr22 Gpr103 Gpr112 Ccr7 Grm4 Sstr5 Adora1 6 L Gipr Ptger3 Lphn2 Ghrhr Adra1d Agtr2 Gabbr2 Avpr1b Gpr6 Mrgprd Chrm2 Mrgprg Chrm5 Mrgprb4 Mrgprb1 Mrgprb3 Mrgprb5 Oxgr1 Gpr27 Gpr35 Taar9 Mrgpra6 Mrgpra2 Ccr4 M Cxcr3 Cxcr4 Ccr6 Ccr8 Il8rb Admr Adrb2 Agtr1a Agtr1b C3ar1 Ccr10 Ccr5 Ccr9 Ccrl1 Cmklr1 Cmkor1 Cnr2 Cx3cr1 Cxcr6 Cysltr1 Cysltr2 Darc Drd1a Ebi2 7 N Edg1 Edg3 Edg5 Edg8 Fprl1 Fzd2 Fzd9 Gpr1 Gpr101 Gpr109a Gpr12 Gpr132 Gpr146 Gpr15 Gpr150 Gpr152 Gpr160 Gpr17 Gpr171 Gpr173 Gpr174 Gpr18 Gpr19 Gpr20 O Gpr21 Gpr23 NULL Gpr30 Gpr33 Gpr34 Gpr39 Gpr41 Ffar2 Gpr55 Gpr62 Gpr65 Npbwr1 Gpr84 Gpr88 Gpr92 Htr1f NULL Ltb4r1 Mrgpra1 Mrgpra3 Mrgpra5 Mrgpra7 Mrgpra8 8 P Mrgprb2 Mrgpre Mrgprf Mrgprx2 Npy2r Npy5r Oxtr P2ry10 P2ry12 P2ry13 P2ry14 P2ry2 P2ry6 Ptafr Sucnr1 Calm2 Adrbk1 Prkaca Adrbk2 Rho Calm1 Rgr Prkacb Rrh 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Port Rat GPCR Array A Adcyap1r1 Admr Adora1 Adora2a Adora2b Adora3 Adra1a Adra1b Adra1d Adra2a 18S 18S Adra2b Adra2c Adrb1 Adrb2 Adrb3 Adrbk1 Adrbk2 Agtr1a Agtr1b Agtr2 Agtrl1 Alox12 1 B Alox5 Avpr1a Avpr1b Avpr2 Bai1 Bai2 Bai3 Bdkrb1 Bdkrb2 Blr1 18S 18S Brs3 C3ar1 C5r1 Calcr Calcrl Casr Ccbp2 Cckar Cckbr Ccr1 Ccr2 Ccr3 C Ccr4 Ccr5 Ccr6 Ccr7 Ccr8 Ccr9 Ccrl2 Cd97 Celsr1 Celsr2 Celsr3 Chrm1 Chrm2 Chrm3 Chrm4 Chrm5 Cmklr1 Cmkor1 Cnr1 Cnr2 Crcp Crhr1 Crhr2 Cx3cr1 2 D Cxcr3 Cxcr4 Cysltr1 Cysltr2 Drd1a Drd2 Drd3 Drd4 Drd5 Edg1 Edg2 Edg5 Edg6 Edg7 Edg8 Ednra Ednrb Eltd1 Emr1 Emr4 F2r F2rl1 F2rl2 F2rl3 E Ffar1 Fpr1 Frzb Fshr Fzd1 Fzd2 Fzd3 Fzd4 Fzd5 Fzd6 Fzd7 Fzd9 Gabbr1 Gabbr2 Galr1 Galr2 Galr3 Gcgr Ghrhr Ghsr Gipr Glp1r Glp2r Gnrhr 3 F Gpbar1 Gpcr12 Gpr1 Gpr101 Gpr103 Gpr108 Gpr109a Gpr110 Gpr112 Gpr113 Gpr114 Gpr115 Gpr116 Gpr119 Gpr120 Gpr123 Gpr125 Gpr126 Gpr128 Gpr135 Gpr137b Gpr139 Gpr141 Gpr143 G Gpr149 Gpr151 Gpr153 Gpr155 Gpr156 Gpr157 Gpr158 Gpr160 Gpr162 Gpr173 Gpr175 Gpr176 Gpr19 Gpr20 Gpr22 Gpr25 Gpr26 Gpr27 Gpr3 Gpr30 Gpr33 GPR34 Gpr35 Gpr37 4 H Gpr37l1 Gpr4 Gpr41 Gpr43 Gpr44 Gpr50 Gpr56 Gpr58 Gpr6 Gpr61 Gpr64 Gpr68 Gpr77 Gpr83 Gpr85 Gpr87 Gpr88 Gpr89 Gpr97 Gprc5a Gprc5b Gprc5c Gprc6a Grm1 I Grm2 Grm3 Grm4 Grm5 Grm6 Grm7 Grm8 Grpr Hcrtr1 Hcrtr2 Hpgd Hrh1 Hrh2 Hrh3 Hrh4 Htr1a Htr1b Htr1d Htr1f Htr2a Htr2b Htr2c Htr4 Htr5a 5 LOC LOC LOC J Htr5b Htr6 Htr7 Icam1 Il8ra Il8rb Kiss1r Kng1 Lancl1 Lgr4 Lgr5 Lgr7 Lgr8 Lhcgr 306792 362068 498289 Lphn1 Lphn2 Lphn3 Lta4h Ltb4r Ltb4r2 Ltc4s K Mapk1 Mapk14 Mas1 Mc2r Mc3r Mc4r Mc5r Mchr1 MGC Mrga10 Mrgprb1
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    BMJ Publishing Group Limited (BMJ) disclaims all liability and responsibility arising from any reliance Supplemental material placed on this supplemental material which has been supplied by the author(s) J Immunother Cancer Supplementary Table S5. Differentially expressed gene lists of PD-1high CD39+ CD8 TILs according to 4-1BB expression compared to PD-1+ CD39- CD8 TILs Up- or down- regulated genes in Up- or down- regulated genes Up- or down- regulated genes only PD-1high CD39+ CD8 TILs only in 4-1BBneg PD-1high CD39+ in 4-1BBpos PD-1high CD39+ CD8 compared to PD-1+ CD39- CD8 CD8 TILs compared to PD-1+ TILs compared to PD-1+ CD39- TILs CD39- CD8 TILs CD8 TILs IL7R KLRG1 TNFSF4 ENTPD1 DHRS3 LEF1 ITGA5 MKI67 PZP KLF3 RYR2 SIK1B ANK3 LYST PPP1R3B ETV1 ADAM28 H2AC13 CCR7 GFOD1 RASGRP2 ITGAX MAST4 RAD51AP1 MYO1E CLCF1 NEBL S1PR5 VCL MPP7 MS4A6A PHLDB1 GFPT2 TNF RPL3 SPRY4 VCAM1 B4GALT5 TIPARP TNS3 PDCD1 POLQ AKAP5 IL6ST LY9 PLXND1 PLEKHA1 NEU1 DGKH SPRY2 PLEKHG3 IKZF4 MTX3 PARK7 ATP8B4 SYT11 PTGER4 SORL1 RAB11FIP5 BRCA1 MAP4K3 NCR1 CCR4 S1PR1 PDE8A IFIT2 EPHA4 ARHGEF12 PAICS PELI2 LAT2 GPRASP1 TTN RPLP0 IL4I1 AUTS2 RPS3 CDCA3 NHS LONRF2 CDC42EP3 SLCO3A1 RRM2 ADAMTSL4 INPP5F ARHGAP31 ESCO2 ADRB2 CSF1 WDHD1 GOLIM4 CDK5RAP1 CD69 GLUL HJURP SHC4 GNLY TTC9 HELLS DPP4 IL23A PITPNC1 TOX ARHGEF9 EXO1 SLC4A4 CKAP4 CARMIL3 NHSL2 DZIP3 GINS1 FUT8 UBASH3B CDCA5 PDE7B SOGA1 CDC45 NR3C2 TRIB1 KIF14 TRAF5 LIMS1 PPP1R2C TNFRSF9 KLRC2 POLA1 CD80 ATP10D CDCA8 SETD7 IER2 PATL2 CCDC141 CD84 HSPA6 CYB561 MPHOSPH9 CLSPN KLRC1 PTMS SCML4 ZBTB10 CCL3 CA5B PIP5K1B WNT9A CCNH GEM IL18RAP GGH SARDH B3GNT7 C13orf46 SBF2 IKZF3 ZMAT1 TCF7 NECTIN1 H3C7 FOS PAG1 HECA SLC4A10 SLC35G2 PER1 P2RY1 NFKBIA WDR76 PLAUR KDM1A H1-5 TSHZ2 FAM102B HMMR GPR132 CCRL2 PARP8 A2M ST8SIA1 NUF2 IL5RA RBPMS UBE2T USP53 EEF1A1 PLAC8 LGR6 TMEM123 NEK2 SNAP47 PTGIS SH2B3 P2RY8 S100PBP PLEKHA7 CLNK CRIM1 MGAT5 YBX3 TP53INP1 DTL CFH FEZ1 MYB FRMD4B TSPAN5 STIL ITGA2 GOLGA6L10 MYBL2 AHI1 CAND2 GZMB RBPJ PELI1 HSPA1B KCNK5 GOLGA6L9 TICRR TPRG1 UBE2C AURKA Leem G, et al.
  • Molecular Characterization of Clonal Human Renal Forming Cells Cohen-Zontag Osnat , Gershon Rotem , Harari-Steinberg Orit , Kant

    Molecular Characterization of Clonal Human Renal Forming Cells Cohen-Zontag Osnat , Gershon Rotem , Harari-Steinberg Orit , Kant

    bioRxiv preprint doi: https://doi.org/10.1101/2020.03.05.978254; this version posted March 6, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Molecular characterization of clonal human renal forming cells Cohen-Zontag Osnat1,7,*, Gershon Rotem1,7,*, Harari-Steinberg Orit1,7,*, Kanter Itamar4, Omer Dorit1,7 , Pleniceanu Oren1,7, Tam Gal4, Oriel Sarit4, Ben-Hur Herzl8,9 , Katz Guy1,3,5,7, Zohar Dotan2,7, Kalisky Tomer4,#, Dekel Benjamin1, 6,7,#,^, Pode- Shakked Naomi1,3,7,#. 1Pediatric Stem Cell Research Institute, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, Tel-Hashomer, Israel 2Dept of Urology, Sheba Medical Center, Tel-Hashomer, Israel 3The Talpiot Medical Leadership Program, Sheba Medical Center, Tel-Hashomer, Israel 4Faculty of Engineering and Bar-Ilan Institute of Nanotechnology and Advanced Materials (BINA), Bar-Ilan University, Ramat Gan, Israel. 5The Joseph Buchman Gynecology and Maternity Center, Sheba Medical Center, Tel- Hashomer, Israel 6Division of Pediatric Nephrology, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, Tel-Hashomer, Israel 7Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel 8L.E.M. Laboratory of Early Detection, Nes Ziona, Israel 9Department of Obstetrics and Gynecology, Assaf Harofeh Medical Center, Tzrifin, Israel *These first authors contributed equally to this work #These senior authors contributed equally to this work ^Correspondence: Benjamin Dekel MD, PhD Pediatric Stem Cell Research Institute Edmond & Lily Safra Children's Hospital, Sheba Medical Center E-mails: [email protected] or [email protected] bioRxiv preprint doi: https://doi.org/10.1101/2020.03.05.978254; this version posted March 6, 2020.
  • In-Depth Characterization of the Wnt-Signaling/Β-Catenin Pathway In

    In-Depth Characterization of the Wnt-Signaling/Β-Catenin Pathway In

    Götzel et al. BMC Gastroenterology (2019) 19:38 https://doi.org/10.1186/s12876-019-0957-5 RESEARCH ARTICLE Open Access In-depth characterization of the Wnt- signaling/β-catenin pathway in an in vitro model of Barrett’s sequence Katharina Götzel1, Olga Chemnitzer1, Luisa Maurer1, Arne Dietrich1,2, Uwe Eichfeld1, Orestis Lyros1, Yusef Moulla1, Stefan Niebisch1, Matthias Mehdorn1, Boris Jansen-Winkeln1, Michael Vieth3, Albrecht Hoffmeister4, Ines Gockel1 and René Thieme1* Abstract Background: An altered Wnt-signaling activation has been reported during Barrett’s esophagus progression, but with rarely detected mutations in APC and β-catenin (CTNNB1) genes. Methods: In this study, a robust in-depth expression pattern analysis of frizzled receptors, co-receptors, the Wnt- ligands Wnt3a and Wnt5a, the Wnt-signaling downstream targets Axin2, and CyclinD1, as well as the activation of the intracellular signaling kinases Akt and GSK3β was performed in an in vitro cell culture model of Barrett’s esophagus. Representing the Barrett’s sequence, we used normal esophageal squamous epithelium (EPC-1, EPC-2), metaplasia (CP-A) and dysplasia (CP-B) to esophageal adenocarcinoma (EAC) cell lines (OE33, OE19) and primary specimens of squamous epithelium, metaplasia and EAC. Results: A loss of Wnt3a expression was observed beginning from the metaplastic cell line CP-A towards dysplasia (CP-B) and EAC (OE33 and OE19), confirmed by a lower staining index of WNT3A in Barrett’s metaplasia and EAC, than in squamous epithelium specimens. Frizzled 1–10 expression analysis revealed a distinct expression pattern, showing the highest expression for Fzd2, Fzd3, Fzd4, Fzd5, Fzd7, and the co-receptor LRP5/6 in EAC cells, while Fzd3 and Fzd7 were rarely expressed in primary specimens from squamous epithelium.
  • Human Kidney Clonal Proliferation Disclose Lineage-Restricted Precursor Characteristics

    Human Kidney Clonal Proliferation Disclose Lineage-Restricted Precursor Characteristics

    www.nature.com/scientificreports OPEN Human kidney clonal proliferation disclose lineage‑restricted precursor characteristics Osnat Cohen‑Zontag1,7,10, Rotem Gershon1,7,10, Orit Harari‑Steinberg1,7,10, Itamar Kanter4, Dorit Omer1,7, Oren Pleniceanu1,7, Gal Tam4, Sarit Oriel4, Herzel Ben‑Hur8,9, Guy Katz1,3,5,7, Zohar Dotan2,7, Tomer Kalisky4,11, Benjamin Dekel1,6,7,11* & Naomi Pode‑Shakked1,3,7,11 In‑vivo single cell clonal analysis in the adult mouse kidney has previously shown lineage‑restricted clonal proliferation within varying nephron segments as a mechanism responsible for cell replacement and local regeneration. To analyze ex‑vivo clonal growth, we now preformed limiting dilution to generate genuine clonal cultures from one single human renal epithelial cell, which can give rise to up to 3.4 * ­106 cells, and analyzed their characteristics using transcriptomics. A comparison between clonal cultures revealed restriction to either proximal or distal kidney sub‑lineages with distinct cellular and molecular characteristics; rapidly amplifying de‑diferentiated clones and a stably proliferating cuboidal epithelial‑appearing clones, respectively. Furthermore, each showed distinct molecular features including cell‑cycle, epithelial‑mesenchymal transition, oxidative phosphorylation, BMP signaling pathway and cell surface markers. In addition, analysis of clonal versus bulk cultures show early clones to be more quiescent, with elevated expression of renal developmental genes and overall reduction in renal identity markers, but with an overlapping expression of nephron segment identifers and multiple identity. Thus, ex‑vivo clonal growth mimics the in‑vivo situation displaying lineage‑restricted precursor characteristics of mature renal cells. These data suggest that for reconstruction of varying renal lineages with human adult kidney based organoid technology and kidney regeneration ex‑vivo, use of multiple heterogeneous precursors is warranted.
  • Technical Note, Appendix: an Analysis of Blood Processing Methods to Prepare Samples for Genechip® Expression Profiling (Pdf, 1

    Technical Note, Appendix: an Analysis of Blood Processing Methods to Prepare Samples for Genechip® Expression Profiling (Pdf, 1

    Appendix 1: Signature genes for different blood cell types. Blood Cell Type Source Probe Set Description Symbol Blood Cell Type Source Probe Set Description Symbol Fraction ID Fraction ID Mono- Lympho- GSK 203547_at CD4 antigen (p55) CD4 Whitney et al. 209813_x_at T cell receptor TRG nuclear cytes gamma locus cells Whitney et al. 209995_s_at T-cell leukemia/ TCL1A Whitney et al. 203104_at colony stimulating CSF1R lymphoma 1A factor 1 receptor, Whitney et al. 210164_at granzyme B GZMB formerly McDonough (granzyme 2, feline sarcoma viral cytotoxic T-lymphocyte- (v-fms) oncogene associated serine homolog esterase 1) Whitney et al. 203290_at major histocompatibility HLA-DQA1 Whitney et al. 210321_at similar to granzyme B CTLA1 complex, class II, (granzyme 2, cytotoxic DQ alpha 1 T-lymphocyte-associated Whitney et al. 203413_at NEL-like 2 (chicken) NELL2 serine esterase 1) Whitney et al. 203828_s_at natural killer cell NK4 (H. sapiens) transcript 4 Whitney et al. 212827_at immunoglobulin heavy IGHM Whitney et al. 203932_at major histocompatibility HLA-DMB constant mu complex, class II, Whitney et al. 212998_x_at major histocompatibility HLA-DQB1 DM beta complex, class II, Whitney et al. 204655_at chemokine (C-C motif) CCL5 DQ beta 1 ligand 5 Whitney et al. 212999_x_at major histocompatibility HLA-DQB Whitney et al. 204661_at CDW52 antigen CDW52 complex, class II, (CAMPATH-1 antigen) DQ beta 1 Whitney et al. 205049_s_at CD79A antigen CD79A Whitney et al. 213193_x_at T cell receptor beta locus TRB (immunoglobulin- Whitney et al. 213425_at Homo sapiens cDNA associated alpha) FLJ11441 fis, clone Whitney et al. 205291_at interleukin 2 receptor, IL2RB HEMBA1001323, beta mRNA sequence Whitney et al.