Anubias Barteri Var. Nana Anubias Barteri

Anubias Barteri var. Nana Anubias Barteri var. nana is one of the five varieties of the Anubias Barteri species and certainly the most popular among hobbyists. From the Araceae family, this plant originates from Cameroon. In the wild, it grows on the edge of streams and rivers. It can grow emerged in wet areas or totally submerged. When submerged, A. Barteri var. nana seems to grow slower. Rarely taller than 5”, Anubias nana thrives in a wide range of conditions. Ph range between 6.0 and 9.0, and temperature between 72 and 82. Lighting requirements are not essential for this plant as they will grow in low as well as in strong lighting. Our personal experience showed that this plant will grow flowers when submerged in a medium to strong lighting environment. Flowers can last for a few months in good water conditions. Anubias nana grows quite slowly and rarely grows more than 8 to 10 leaves a year. These same leaves can survive for several years and are prone to algae. That algae can be removed by transferring the contaminated plant in another tank with very different water conditions and low lighting. Because of their easy adaptation to most aquarium environment, Anubias nana are quite easy to keep. They can be kept as foreground plants directly rooted in the sand or gravel. In this case, it is important to not to cover the rhizome as it would rot. Another successful way of growing this plant is to attach it to a piece of rock or root. Use fishing line to secure the plant on the substrate. Roots will develop on the chosen substrate downward. To reproduce this plant, simply divide the rhizome in two pieces and plant/attach it somewhere else in the tank. These plants are known to be very strong and resistant against most herbivorous fish. Another great species to have in your tank for growing off rock and drift. .

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Micropropagation of Anubias Barteri Var. Nana from Shoot Tip Culture and the Analysis of Ploidy Stability
Available online at www.notulaebotanicae.ro Print ISSN 0255-965X; Electronic 1842-4309 Not Bot Horti Agrobo, 2012, 40(2): 148-151 Notulae Botanicae Horti Agrobotanici Cluj-Napoca Micropropagation of Anubias barteri var. Nana from Shoot Tip Culture and the Analysis of Ploidy Stability Kantamaht KANCHANAPOOM1*, Panyaros CHUNUI2 , Kamnoon KANCHANAPOOM2 1 Prince of Songkla University, Faculty of Science, Department of Molecular Biotechnology and Bioinformatics, Hat Yai, Songkhla, 90112 Thailand;[email protected] (*corresponding author) 2 Prince of Songkla University, Faculty of Science, Department of Biology, Plant Biotechnology Research Unit, Hat Yai, Songkhla, 90112 Thailand; [email protected] Abstract Plant regeneration of Anubias barteri var. Nana was achieved through organogenesis in shoot tip cultures. Multiple shoots were induced from cultured shoot tips on a modified MS (Murashige and Skoog, 1962) medium supplemented with BA and kinetin. The maximum green shoot numbers were best obtained on MS medium containing 3 mg/L BA with 5 shoots. Rooting in all regenerated shoots was promoted on MS medium devoid of plant growth regulators or kinetin singly. Acclimatization and survival when transferred to field conditions were shown to be 100% in the regenerated plants. Cytological and flow cytometric analyses of the mother plants and in vitro grown plants derived from 5 years old cultures showed no differences in ploidy level, they were all diploid (2n = 2x = 48) with a 2C peak indicating that ploidy alteration did not occur. Keywords: aquatic plant, Araceae, flow cytometry, nuclear DNA content Introduction Materials and methods Plant materials The genus Anubias of the family Araceae is divided Young plantlets of A.
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