PATZ1 Rabbit Pab

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PATZ1 Rabbit pAb

Catalog No.: A10053

Basic Information Background

Catalog No. The protein encoded by this gene contains an A-T hook DNA binding motif which usually A10053 binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein- Observed MW protein interaction and is required for transcriptional repression, and the zinc-fingers 74kDa comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round Calculated MW cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is 57kDa/69kDa/74kDa thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence Category containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Primary antibody Four alternatively spliced transcript variants are described for this gene. Applications WB

Cross-Reactivity Human, Mouse, Rat

Recommended Dilutions Immunogen Information

WB 1:1000 - 1:4000 Gene ID Swiss Prot 23598 Q9HBE1

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 250-350 of human PATZ1 (NP_114440.1).

Synonyms PATZ1;MAZR;PATZ;RIAZ;ZBTB19;ZNF278;ZSG;dJ400N23

Contact Product Information

www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification

Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data

Western blot analysis of extracts of various cell lines, using PATZ1 antibody (A10053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.