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Hormone Testing Summary Accession # 00280397 Male Sample Report 123 A Street Sometown, CA 90266 Ordering Provider: DOB: 1967-08-09 Collection Times: Precision Analytical Age: 50 2017-08-09 06:01AM (S) 2017-08-09 06:31AM (S) Gender: Male 2017-08-09 07:01AM (S) 2017-08-09 05:01PM (S) 2017-08-09 10:01PM (S) 2017-08-09 06:01AM (U) 2017-08-09 08:01AM (U) 2017-08-09 05:01PM (U) 2017-08-09 10:01PM (U) Hormone Testing Summary Key (how to read the results): Sex Hormones Testosterone Age Range 2.4 25 22 patient 18-25 50-115 low limit high limit result 0.5 2.2 115 26-40 40-95 41-60 30-80 Estradiol(E2) Testosterone >60 25-60 Adrenal Hormones See pages 4 and 5 for a more complete breakdown of adrenal hormones Total DHEA Production 10 1000 5500 Age Range 1317 Saliva Free Cortisol Pattern 20-39 3000-5500 8 40-60 2000-4000 (ng/mL) >60 1000-2500 Total DHEA Production (DHEAS + Etiocholanolone + Androsterone) 6 High Range Limit Patient Values 4 9.60 4550 Saliva Cortisol Saliva 17.48 9250 19.30 10000 2 Low Range Limit Saliva Cortisol Total cortisol Metabolized Cortisol (THF+THE) 0 (Sum of 5 values) metabolism (Total Cortisol Production) Waking (W) W+30 min. W+60 min. Afternoon Night Free cortisol best reflects tissue levels. Metabolized cortisol best reflects total cortisol production. The following videos (which can also be found on the website under the listed names along with others) may aid your understanding: DUTCH Plus Overview (quick overview) Estrogen Tutorial Male Androgen Tutorial Cortisol/CAR Tutorial PLEASE BE SURE TO READ BELOW FOR A NY SPECIFIC LA B COMMENTS. More detailed comments can be found on page 9. - The patient collected an "Insomnia" salivary sample in the middle of the night. The cortisol result for this sample was 2.10ng/mL (expected range 0-0.9). Please see page 4 for cortisol and cortisone results for this sample. The Cortisol Awakening Response (CAR) was 2.99ng/mL (expected range 1.5-4.0) or 69.2% (range 50-160%). See page 5 for more details. Precision Analytical (Dawn Huo, Ph.D., Lab Director) Male Sample Report Page 1 of 18 3138 Rivergate Street #301C FINAL REPORT CLIA Lic. #38D2047310 McMinnville, OR 97128 01/28/2021 DutchTest.com Ref020121 Accession # 00280397 Male Sample Report 123 A Street Sometown, CA 90266 Sex Hormones and Metabolites Ordering Provider: DOB: 1967-08-09 Collection Times: Precision Analytical Age: 50 2017-08-09 06:01AM (S ) 2017-08-09 06:31AM (S ) Gender: Male 2017-08-09 07:01AM (S ) 2017-08-09 05:01PM (S ) 2017-08-09 10:01PM (S ) 2017-08-09 06:01AM (U) 2017-08-09 08:01AM (U) 2017-08-09 05:01PM (U) 2017-08-09 10:01PM (U) Category Test Result Units Normal Range Progesterone Metabolites (Urine) b-Pregnanediol Low end of range 110.0 ng/mg 75 - 400 a-Pregnanediol Low end of range 40.0 ng/mg 20 - 130 Estrogens and Metabolites (Urine) Estrone(E1) High end of range 15.3 ng/mg 4 - 16 Estradiol(E2) Above range 2.4 ng/mg 0.5 - 2.2 Estriol(E3) Within range 4.2 ng/mg 2 - 8 2-OH-E1 Within range 3.73 ng/mg 0 - 5.9 4-OH-E1 Above range 1.2 ng/mg 0 - 0.8 16-OH-E1 Within range 0.7 ng/mg 0 - 1.2 2-Methoxy-E1 Within range 2.1 ng/mg 0 - 2.8 2-OH-E2 Above range 0.61 ng/mg 0 - 0.6 4-OH-E2 Within range 0.1 ng/mg 0 - 0.3 2-Methoxy-E2 Within range 0.3 ng/mg 0 - 0.8 Total Estrogen High end of range 30.34 ng/mg 10 - 34 Androgens and Metabolites (Urine) DHEA-S Low end of range 95.0 ng/mg 30 - 1500 Androsterone Low end of range 835.0 ng/mg 500 - 3000 Etiocholanolone Below range 387.0 ng/mg 400 - 1500 Testosterone Below range 21.6 ng/mg 25 - 115 5a-DHT Low end of range 8.2 ng/mg 5 - 25 5a-Androstanediol Low end of range 52.0 ng/mg 30 - 250 5b-Androstanediol Low end of range 46.0 ng/mg 40 - 250 Epi-Testosterone Low end of range 38.1 ng/mg 25 - 115 Precision Analytical (Dawn Huo, Ph.D., Lab Director) Male Sample Report Page 2 of 18 3138 Rivergate Street #301C FINAL REPORT CLIA Lic. #38D2047310 McMinnville, OR 97128 01/28/2021 DutchTest.com Hormone metabolite results from the previous page are presented here as they are found in the steroid cascade. See the Provider Comments for more information on how to read the results. Age-Dependent Ranges s Age DHEA-S n 20-39 150-1500 e Pregnenolone 40-60 60-800 g >60 30-300 o 30 95 r Etiocholanolone Androsterone d 1500 20-39 800-1500 20-39 1500-3000 n DHEA 40-60 600-1200 40-60 1000-2000 A DHEA-S >60 400-1000 >60 500-1000 5ß-androstanediol 5α-androstanediol 20-39 70-250 20-39 60-250 40-60 55-210 40-60 50-180 Androstenedione 25 22 >60 40-150 >60 30-130 5 ß α 5 a 115 Testosterone 5α-DHT ro m a 18-25 50-115 20-39 9-25 ta s 26-40 40-95 40-60 7-20 e Testosterone 41-60 30-80 >60 5-16 a ro m >60 25-60 a ta 400 387 1500 500 835 3000 se Etiocholanolone Androsterone Testosterone 5 ß α 15.3 2.4 5 4.2 4.0 16.0 0.5 2.2 2.0 8.0 CYP3A4 Estrone(E1) Estradiol(E2) Estriol(E3) Less Androgenic Metabolites primary estrogens (E1, E2, E3) 5.0 8.2 C Y P 3 25.0 A 4 5a-DHT s n 0.7 e 1.2 g 0.0 o C 40 30 Y 16-OH-E1 ) Phase 1 Estrogen Metabolism Ratios 46 52 r P y 1 a t 250 250 B w 1 s h t a E 5b-Androstanediol 5a-Androstanediol p e v i t c e Patient t 2-OH 4-OH 16-OH o Percentages 5ß preference 5α Preference r 66.2% 21.4% 12.4% p (androgenic) ( Expected 60-80% 7.5-11% 13-30% 1 A Percentages (2-OH) (4-OH) (16-OH) 5α-Reductase Activity 1 1.2 P Y 0.8 C 5α-metabolism makes androgens more potent, most 0.0 notably 5α-DHT is the most potent testosterone metabolite 4-OH-E1 Glutathione detox Low High COMT QUINONE 2.1 3.73 (reactive) 2.8 methylation 5.90 Methylation-activity 0.0 0.00 2-Methoxy-E1 2-OH-E1 2-Methoxy/2-OH Methylation detox 4-OH-E1 If not detoxified, 4-OH-E1 can bind to and damage DNA Precision Analytical (Dawn Huo, Ph.D., Lab Director) Male Sample Report Page 3 of 18 3138 Rivergate Street #301C FINAL REPORT CLIA Lic. #38D2047310 McMinnville, OR 97128 01/28/2021 DutchTest.com Accession # 00280397 Male Sample Report 123 A Street Sometown, CA 90266 Adrenal Ordering Provider: DOB: 1967-08-09 Collection Times: Precision Analytical Age: 50 2017-08-09 06:01AM (S ) 2017-08-09 06:31AM (S ) Gender: Male 2017-08-09 07:01AM (S ) 2017-08-09 05:01PM (S ) 2017-08-09 10:01PM (S ) 2017-08-09 01:31AM (S *) 2017-08-09 06:01AM (U) 2017-08-09 08:01AM (U) 2017-08-09 05:01PM (U) 2017-08-09 10:01PM (U) Category Test Result Units Normal Range Free Cortisol and Cortisone (Saliva) Saliva Cortisol - Waking (W) High end of range 4.32 ng/mL 1.6 - 4.6 Saliva Cortisol - W+30 min. High end of range 7.31 ng/mL 3.7 - 8.2 Saliva Cortisol - W+60 min. Within range 4.33 ng/mL 2.3 - 5.3 Saliva Cortisol - Afternoon Within range 1.2 ng/mL 0.4 - 1.5 Saliva Cortisol - Night Within range 0.32 ng/mL 0 - 0.9 Saliva Cortisone - Waking (W) High end of range 13.16 ng/mL 6.8 - 14.5 Saliva Cortisone - W+30 min. Above range 25.57 ng/mL 12.4 - 19.4 Saliva Cortisone - W+60 min. Above range 16.9 ng/mL 9.4 - 15.3 Saliva Cortisone - Afternoon Above range 7.98 ng/mL 2 - 7.1 Saliva Cortisone - Night Within range 2.02 ng/mL 0 - 4.8 Saliva Cortisol Total High end of range 17.48 ng/mL 9.6 - 19.3 Saliva Cortisone Total Above range 65.63 ng/mL 36 - 55 Creatinine (Urine) Creatinine A (Waking) Within range 0.45 mg/ml 0.3 - 3 Creatinine B (Morning) Within range 0.41 mg/ml 0.3 - 3 Creatinine C (Afternoon) Within range 0.9 mg/ml 0.3 - 3 Creatinine D (Night) Within range 0.88 mg/ml 0.3 - 3 Cortisol Metabolites and DHEA-S (Urine) a-Tetrahydrocortisol (a-THF) Within range 450.0 ng/mg 175 - 700 b-Tetrahydrocortisol (b-THF) Within range 2800.0 ng/mg 1750 - 4000 b-Tetrahydrocortisone (b-THE) Above range 6000.0 ng/mg 2350 - 5800 Metabolized Cortisol (THF+THE) High end of range 9250.0 ng/mg 4550 - 10000 DHEA-S Low end of range 95.0 ng/mg 30 - 1500 Additional Cortisol and Cortisone (Saliva) * Saliva Cortisol - Insomnia Above range 2.1 ng/mL 0 - 0.9 * Saliva Cortisone - Insomnia Above range 10.4 ng/mL 0 - 4.8 Precision Analytical (Dawn Huo, Ph.D., Lab Director) Male Sample Report Page 4 of 18 3138 Rivergate Street #301C FINAL REPORT CLIA Lic.
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