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Ilhan Esse # 11 Library Construction Effi Ciency in KAPA Library Preparation and KAPA Hyper Prep Kits Dana-Farber/Harvard Cancer Center CURE Continuing Umbrella of Research Experiences Scientifi c Presentations Summer 2015 I Dana-Farber/Harvard Cancer Center Continuing Umbrella of Research Experiences (CURE) Launched in 2002, the Continuing Umbrella of Research Experiences (CURE) at Dana-Farber/Harvard Cancer Center (DF/HCC) is an important building block in research training initiatives. Under the direction of the DF/HCC Initiative to Eliminate Cancer Disparities(IECD), this program is designed to provide underrepresented minority high school and college students with a stimulating and rewarding hands-on research experience that encourages students to pursue education and training in the biomedical sciences and careers in basic, clinical, nursing, and population sciences, cancer research. Dana-Farber/Harvard Cancer Center Initiative to Eliminate Cancer Disparities (IECD) Th e IECD provides a centralized and coordinated structure for addressing cancer disparities. It was among the nation’s fi rst integrated, inter- institutional, multi-pronged approach for addressing cancer inequities. Its mission is to support and encourage disparities research in all disciplines and across all DF/HCC member institutions. Th e IECD focuses on four key areas: a) community engagement, b) training, recruitment and faculty development, c) increasing minority enrollment to cancer clinical trials, and d) enhancing cultural competency throughout DF/HCC institutions and membership. For more information about the IECD or CURE program, contact: Karen Burns White, Deputy Associate Director Initiative to Eliminate Cancer Disparities P: (617) 632-3244, E: [email protected] Ying Jiang, CURE Research Training Specialist P: (617) 632-3028, E: [email protected] CURE Scientifi c Presentations Oral Presentations Tuesday, August 11, 2015 and Wednesday, August 12, 2015 2:30P.M.-5:00 P.M. Digital Poster Presentations Thursday, August 13, 2015 3:30P.M.-5:00 P.M. Dana-Farber Cancer Institute 450 Brookline Avenue, Third Floor Yawkey Conference Center Boston, MA 02215 Table of Contents Usman Ali Ahmed 1 Rachel C. Ezieme 12 Detecting Binary Protein-Protein Interactions Using Understanding Barriers to Cancer Care Among the Yeast Two-Hybrid Assay Black Bostonians Kate Armstrong 2 Armel Foade 13 Examining Oncogenic Transformation of Transfected Towards Nanoparticle Drones: New Technology FLT3 Gene Isoform in Hek293T Cell Line to Kill Deadly Prostate Cancer Cells with Minimal Collateral Damage Omar Badr 3 Involvement of Signal Transduction Molecules in Nora Fortoul 14 Cardiomyocyte (CM) Proliferation Patient Ancestry May be a Determinant in Hematopoietic Stem Cell Transplantation Katisha Bellegarde 4 Outcomes The Effect of Cyclin Dependent Kinases on the Expression and Function of Enhancer of Zest Ana Paola Garcia # 15 Homolog 2 (EZH2) and its Implications on Foxp3 The Interplay between Disrupted Peripheral expression Circadian Rhythmicity and Cancer Cachexia Roderick Brathwaite Jr. # 5 Heresa Guerrier # 16 Weight Loss in Overweight and Obese A Comparison of Two CRISPR-Cas9-based Postmenopausal Women Reduces Breast Cancer Methods for Transcriptional Activation in Drosophila Risk Soham Gupta 17 Kaelyn Brown # 6 Reduction in Prostate Specifi c Antigen Screening Investigating the Role of NRDE-2 in Non-Small Cell Following Contrary Recommendation Lung Cancer Adnil Gutarra # 18 Piseth Cheav 7 Cancer Genome Atlas Gene Mutation: Detection of Investigating the Reproducibility of Tumor Co-occurring Variants and Network Community Measurements Between Different Image Analysts Ted Hilaire # 19 Daniella Colombo 8 Angiogenic Properties of Breast Cancer Cell Lines BPA-Induced Stress Granules: Characterizing the Cellular Stress Response Lisa Hsiao 20 Müllerian Inhibiting Substance Inhibits Growth of Eliezer Colon 9 Ovarian Cancer Cells In Vitro Eicosapentaenoic Acid Induces Survival of Human Skin Fibroblasts Exposed to Ultraviolet Light Alexander R. Jeremiah # 21 Epidermal Growth Factor Receptor Inhibition for Kenesha Darlington # 10 Targeted Neurofi bromatosis Therapies Analysis of Intestinal Pro- and Anti-infl ammatory Transcription Factor Expression after Sleeve Thamael Laurore # 22 Gastrectomy The Use of Functional Magnetic Resonance Imaging (fMRI) to Prevent Post Operative Defi cits Ilhan Esse # 11 Library Construction Effi ciency in KAPA Library Preparation and KAPA Hyper Prep Kits # Indicates students presenting a poster Nicholas F. Lawrence 23 Emilio Vides-Curnen # 34 Id1 and p53 Expression and G691S RET Red Blood Cell Antigen Prediction Using Whole Polymorphism in Desmoplastic Melanoma: An Genome Sequencing (WGS) Data Analysis of Sixty Six Cases Carolina Villalba 35 Amy Luong 24 Neuropilin 2 Infl uences Angiogenesis in Breast Correlating Distances between Prostate Cancer Biopsy Core and Cancer Lesion to Respective Metabolomic Profi les Katherina Yeager # 36 BRG/BRM Assay Development Pipeline Khang Nguyen # 25 Defective NHEJ Leads to Deregulated PI3-K Pathway in Glioblastoma Multiforme Mice Model Habiba Noamany 26 Structural Study of Actin Nucleating Formins using X-Ray Crystallography Miracle Onyeoziri # 27 Drugs (263,1781) Inhibit the Hypoxia Induced Cell Death Ambar Piña 28 Optimization of Cell Migration Assay to Assess the Role of CMG-2 in Endothelial Cell Migration Brenda Romero # 29 DREAM Complex Maintenance in Quiescence is Regulated by CDK Phosphorylation Naria Sealy 30 Questionnaire Reliability in the Boston Mammography Cohort Study Adam Stevens 31 Exploring Blood Platelets as a Liquid Biopsy for Tumor-Derived Mutant RNA in Patients with Glioblastoma Mariano R. Suriel 32 A Phase I/Ib Study of Ipilimumab in Patients with Relapsed Hematologic Malignancies after Allogeneic Hematopoietic Cell Transplantation Judene Thomas # 33 Numb Isoform Expression During MEL (Murine Erythroleukemia) DMSO (Dimethyl sulfoxide) -induced Differentiation Detecting Binary Protein-Protein Interactions Using the Yeast Two-Hybrid Assay Usman Ali Ahmed Principal Investigator: Marc Vidal, PhD Mentors: Tiziana Cafarelli, PhD; Alice Desbuleux Dana-Farber Cancer Institute Macromolecules are involved in almost every function of a cell and tend to interact with each other in networks. Studying such macromolecular networks can help us understand genotype-phenotype relationships in living organisms. Perturbations occurring within these macromolecular networks can lead to complex disease phenotypes such as cancer. In order to improve our understanding of the relationships between macromolecules, specifi cally proteins, the Center for Cancer Systems Biology systematically maps complete and high-quality protein- protein interactions called “interactomes,” of various model organisms. Due to its evolutionarily well conserved pathways and easily manipulable genetics, we are currently mapping the interactome of the simple model eukaryote, Saccharomyces cerevisiae using the Yeast Two-Hybrid (Y2H) assay. In this assay, proteins of interest X and Y, are respectively fused to the DNA binding domain (DB) and activation domain (AD) of the transcription factor, Gal4. If an interaction occurs between these proteins, Gal4 is reconstituted, which then activates a reporter gene, allowing the yeast cells containing the interacting proteins to grow on selective media. Our pipeline screens ~6,000 AD prey proteins against ~6,000 DB bait proteins which covers a subset of the entire interactome. In our latest screen of a subset of bait and prey protein, we identifi ed a total of 1,263 possible interactions; these will later be verifi ed by a pairwise Y2H assay and subsequently validated with other orthogonal assays. Generating a high-quality, binary Saccharomyces cerevisiae interactome map will provide a deeper understanding of protein networks and through the study of their perturbations, insight into genetic variation, including disease. 1 Examining Oncogenic Transformation of Transfected FLT3 Gene Isoform in Hek293T Cell Line Kate Armstrong Principal Investigator: James D Griffi n, MD Mentor: Sophia Adamia, PhD Dana-Farber Cancer Institute Introduction: Acute myeloid leukemia (AML) is the most prevalent leukemia in adults. AML begins in blood stem cells in the bone marrow but is quick to spread, resulting in a low survival rate. Th ese outcomes have initiated the search for novel biomarkers and drug targets for selective therapy. Previously in our laboratory, genetic alterations were identifi ed in the FLT3 gene; this gene is known to be involved in hematopoesis and cell cycle regulation and we are currently testing the biological eff ects of these alterations in a cell line model system. We engineered the FLT3 gene transfected into HEK293T cells and are examining if the genetically altered FLT3 gene is transforming and has oncogenic characteristics. Study Hypothesis: It was hypothesized that because of genetic alterations detected on the FLT3 gene, the novel form of this gene would encode a novel form of FLT3 protein with oncogeneic potential. Methods: HEK293T cells were cultured and split prior to transfection. Previously generated plasmids containing the FLT3 gene isoform were transfected into the HEK293T cells using Lipofectamine 2000 Reagent. Polymerase chain reaction (PCR) and western blotting were conducted, and gel electrophoresis was performed to evaluate successful gene delivery. Results: Our Real-Time PCR (RT-PCR) and western blotting analyses demonstrate that novel transcripts of FLT3 were successfully expressed in the HEK293T cells. We are currently monitoring biological eff ects of novel
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