DOCSLIB.ORG

Cytotoxic and Anti-Inflammatory Compounds from Red Sea Grass

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Cytotoxic and Anti-Inflammatory Compounds from Red Sea Grass MEDICINAL Medicinal Chemistry Research CHEMISTRY https://doi.org/10.1007/s00044-018-2143-7 RESEARCH ORIGINAL RESEARCH Cytotoxic and anti-inflammatory compounds from Red Sea grass Thalassodendron ciliatum 1,2 2 1 2 Reda F. Abdelhameed ● Amany K. Ibrahim ● Koji Yamada ● Safwat A. Ahmed Received: 24 October 2017 / Accepted: 23 January 2018 © Springer Science+Business Media, LLC, part of Springer Nature 2018 Abstract Chemical investigation of the less polar fraction methylene dichloride–methanol extract of the Red Sea grass Thalassodendron ciliatum led to the isolation of a new phytoceramide molecular species TCC-1, along with four known compounds: 7β-hydroxy cholesterol (10), 7β-hydroxysitosterol (11), stigmasterol glucoside (12), and β-sitosterol glucoside (13). Phytosphingosines with 2-hydroxy fatty acid residues constituted the phytoceramide molecular species TCC-1. Further purification of TCC-1 afforded two new phytoceramides: TCC-1-5 (5) and TCC-1-7 (7) as well as the known ceramide TCC- 1-6 (6). All compounds are reported for the first time from this genus. The chemical structures of the isolated compounds were clarified on the basis of spectroscopic techniques including IR, NMR experiments, mass spectrometry, and chemical fi 1234567890();,: methods, in addition to comparison with literature data. All isolated compounds exhibited signi cant cytotoxicity against two human cell lines (Hep G2 and MCF-7). Moreover, compounds (10–13) have been found to possess significant anti- inflammatory activity in which compound (13) is the most potent. Keywords Thalassodendron ciliatum ● Ceramide ● Cytotoxic compounds ● anti-inflammatory compounds Introduction marine vascular flowering plants that are widely distributed along the coastal and estuarine regions of the world. They Ethnobotanical studies have continued to validate folkloric are unique in that they are often completely submerged in use of plants (marine and terrestrial) (Moghadamtousi et al. water. There are approximately 12 genera affording 2015) in phytomedicinal remedies (Rivera et al. 2005); 50 species of sea grasses. Seven genera are tropical, while (Sharma and Gupta 2015). Now, more than any time, in the the remaining five are almost confined to temperate waters history of phytochemistry research, the chemical basis of (Pollard and Greenway 1993). In many places, sea grasses most age-long folkloric cures is better understood. Drug cover extensive areas, which are usually known as sea grass development in contemporary times have benefitted from beds. The sea grass beds play a vital role in the marine these bodies of knowledge, accounting for more than 50% ecosystem since they act as shelter for a variety of other of drugs and other pharmaceutical preparations approved organisms and provide food for commercially important fish for human use today (Sharma and Gupta 2015) for the species (Howard et al. 1989). McMillan (McMillan et al. treatment and/or management of diseases. Sea grasses are 1980) reported that sea grass is a rich source of secondary metabolites, especially phenolics, which are known to have various biological activities. One such plant whose bioac- tive principles are under intensive research currently is Electronic supplementary material The online version of this article Thalassodendron ciliatum. T. ciliatum is a tropical sea grass (https://doi.org/10.1007/s00044-018-2143-7) contains supplementary fi material, which is available to authorized users. which can be classi ed as sub-tidal and not deep water bed- forming sea grass (Carruthers et al. 2002). It is widely * Safwat A. Ahmed distributed in the Red Sea, the tropical part of the [email protected] Indo–Pacific region, and the western Indian Ocean (Den and 1 Graduate School of Biomedical Sciences, Nagasaki University, Hartog 1970). Recently, its phenolic constituents (rutin, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan asebotin, 3-hydroxyasebotin, quercetin-3-O-beta-D-xylo- 2 Departments of Pharmacognosy, Faculty of Pharmacy, Suez Canal pyranoside, catechin, and caffeic acid) were isolated and University, Ismailia 41522, Egypt characterized (Hamdy et al. 2012) and assayed for cytotoxic Medicinal Chemistry Research response in HCT-116, HEPG, MCF-7, and HeLa human Plant material, collection, and identification cancer cell lines. Furthermore, three independent studies have established that the extract of the plant and its isolated The sea grass T. ciliatum (coll. no. SAA-41) was collected components (especially asebotin) have potent anti-viral from Sharm el sheikh at the Egyptian Red Sea, air-dried, activities (Mohammed et al. 2014); (Ibrahim et al. 2013). and stored at low temperature (−24 ˚C) until processed. The Anti-oxidant activities have also been reported due to the plant was identified by Dr Tarek Temraz, Marine Science presence of flavonoids. Department, Faculty of Science, Suez Canal University, A limited number of phytochemical studies are found on Ismailia, Egypt, A voucher specimen was deposited in the the isolation and structure elucidation of the chemical herbarium section of Pharmacognosy Department, Faculty constituents from the polar fractions of the Red Sea grass of Pharmacy, Suez Canal University, Ismailia, Egypt under Thalassodendron ciliatum and to the best of our knowledge registration number SAA-41. there is no chemical investigation for the non polar fraction till now, except for the diglyceride ester isolated by our Extraction and isolation group (Ibrahim et al. 2013). Therefore, in this study we have further expanded the chemical landscape of the Red T. ciliatum was dried (250 g dry weight), grounded, and Sea grass T. ciliatum with the isolation and spectral char- repeatedly extracted with a mixture of CH2Cl2/MeOH (1:1) acterization of phytosphingosine type ceramides and ster- (3 × 2 L) at room temperature. The combined extracts were oidal scaffolds for the first time. concentrated under vacuum to afford a dark green residue TC (30 g). The residue was chromatographed over silica gel column using CHCl3: MeOH: H2O (100:0:0 ~ 60:40:10) Material and methods gradient elution to give 11 sub-fractions (TC-1–TC-11) based on TLC analysis. Fraction TC-6 (0.64 g), was sub- General experimental procedures jected to SiO2 column eluting with a gradient of increasing MeOH in CHCl3 to afford 6 fractions (TC-6-1 ~ TC-6-6). 1H-NMR chemical shifts are expressed in δ values refer- Fraction TC-6-4 (114 mg) was chromatographed over ring to the solvent peak δH 7.19, 7.55, and 8.71 for Pyr- Sephadex LH-20 column and eluted with CHCl3: MeOH idine-d5, 7.26 for CDCl3, and coupling constants are (1:1) to yield 4 fractions (TC-6-4-1 ~ TC-6-4-4). Fraction expressed in Hz. 13C NMR chemical shifts are expressed TC-6-4-3 (56 mg) was successively re-chromatographed in δ values referring to the solvent peak δC 123.5, 135.5, over silica gel column using CHCl3: MeOH (9.5:0.5) iso- 1 13 and 149.9 for Pyridine-d5, and 77 for CDCl3. Hand C cratic elution to give a pure phytoceramide molecular spe- NMR spectra were recorded with a Unity plus 400 spec- cies TCC-1 (28 mg). TCC-1 (23 mg) was finally purified on trometer (Varian Inc., U.S.A.) operating at 400 MHz for semi-preparative HPLC (cosmosil 5C18, 100% MeOH) to 1H, and 100 MHz for 13C. Optical rotations were mea- afford TCC-1-1 (1) (0.3 mg), TCC-1-2 (2) (0.9 mg), TCC-1- sured with a JASCO DIP-370 digital polarimeter. Positive 3(3) (0.6 mg), TCC-1-4 (4) (0.8 mg), TCC-1-5 (5) (2.1 mg), ion FAB-MS spectra were recorded on JMS DX-303 TCC-1-6 (6) (6.4 mg), TCC-1-7 (7) (2.4 mg), TCC-1-8 (8) spectrometer (JEOL Ltd., Japan), using m-nitrobenzyl (1.3 mg), and TCC-1-9 (9) (1.0 mg) as pure phytocer- alcohol or Magic bullet as a matrix. Sephadex LH-20 amides. Fraction TC-4 (1.4 g), was further chromatographed (Pharmacia Fine Chemical Co.Ltd) and Wakogel C-300 over SiO2 column using CHCl3: MeOH (100:0 ~ 80:20) (Wako Pure Chemical Industries, Ltd, Japan, 45 ~ 75 μm) gradient elution to afford 10 fractions (TC-4-1 ~ TC-4-10). were used for column chromatography. Precoated silica Fraction TC-4-5 (320 mg) was chromatographed over gel plates (Merck, Kieselgel 60 F254, 0.25 mm) and RP- Sephadex LH-20 column and eluted with CHCl3: MeOH 18 F254s plates (Merck) were used for thin-layer chro- (1:1) followed by silica gel column using n-hexane: EtOAc matography (TLC) analysis. IR spectra were obtained (70:30 ~ 50:50) to yield 6 fractions (TC-4-5-1 ~ TC-4-5-6). with JASCO FT/IR-410 spectrophotometers. The UV Fraction TC-4-5-3 (65 mg) was chromatographed over spectra were recorded on a double beam Shimadzu silica gel column using n-hexane: EtOAc (70:30) isocratic UV–visible spectrophotometer (model UV-1601 PC, elution followed by semi-preparative reversed-phase HPLC Kyoto City, Japan). Semi-preparative high-performance (cholester, 100% MeOH) to give 10 (2.5 mg) and 11 (2.2 liquid chromatography (HPLC) was performed on a cos- mg). Fraction TC-7 (0.6 g), was subjected to silica gel mosil 5C18–MS–II (150 × 4.6 mm) at a flow rate of 0.5 ml/ column using CHCl3: MeOH (100:0 ~ 90:10) gradient elu- min, and cholester column at a flow rate of 0.5 ml/min. tion to afford seven fractions (TC-7-1 ~ TC-7-7). Com- Both columns were equipped with a TOSOH RI-8020 pounds 12 (7.7 mg) and 13 (13.5 mg) were isolated after detector and a JASCO BIP-I HPLC pump. column chromatography over Sephadex LH-20 column and Medicinal Chemistry Research Table 1 1H and 13C-NMR spectral data of TCC-1 (δ values in C D N)a eluted with CHCl3: MeOH (1:1) followed by semi- 5 5 preparative reversed-phase HPLC (cholester, 90% MeOH) Position TCC-1 from fraction TC-7-5 (66 mg).
Load more

Recommended publications
  • Global Seagrass Distribution and Diversity: a Bioregional Model ⁎ F
    Journal of Experimental Marine Biology and Ecology 350 (2007) 3–20 www.elsevier.com/locate/jembe Global seagrass distribution and diversity: A bioregional model ⁎ F. Short a, , T. Carruthers b, W. Dennison b, M. Waycott c a Department of Natural Resources, University of New Hampshire, Jackson Estuarine Laboratory, Durham, NH 03824, USA b Integration and Application Network, University of Maryland Center for Environmental Science, Cambridge, MD 21613, USA c School of Marine and Tropical Biology, James Cook University, Townsville, 4811 Queensland, Australia Received 1 February 2007; received in revised form 31 May 2007; accepted 4 June 2007 Abstract Seagrasses, marine flowering plants, are widely distributed along temperate and tropical coastlines of the world. Seagrasses have key ecological roles in coastal ecosystems and can form extensive meadows supporting high biodiversity. The global species diversity of seagrasses is low (b60 species), but species can have ranges that extend for thousands of kilometers of coastline. Seagrass bioregions are defined here, based on species assemblages, species distributional ranges, and tropical and temperate influences. Six global bioregions are presented: four temperate and two tropical. The temperate bioregions include the Temperate North Atlantic, the Temperate North Pacific, the Mediterranean, and the Temperate Southern Oceans. The Temperate North Atlantic has low seagrass diversity, the major species being Zostera marina, typically occurring in estuaries and lagoons. The Temperate North Pacific has high seagrass diversity with Zostera spp. in estuaries and lagoons as well as Phyllospadix spp. in the surf zone. The Mediterranean region has clear water with vast meadows of moderate diversity of both temperate and tropical seagrasses, dominated by deep-growing Posidonia oceanica.
    [Show full text]
  • Reassessment of Seagrass Species in the Marshall Islands1
    Micronesica 2016-04: 1–10 Reassessment of Seagrass Species in the Marshall Islands 1 ROY T. TSUDA Department of Natural Sciences, Bishop Museum, 1525 Bernice Street, Honolulu, HI 96817, USA [email protected] NADIERA SUKHRAJ U.S. Fish and Wildlife Service, Pacific Islands Fish and Wildlife Office, 300 Ala Moana Blvd., Honolulu, HI 96850, USA [email protected] Abstract—Recent collections of specimens of Halophila gaudichaudii J. Kuo, previously identified as Halophila minor (Zollinger) den Hartog, from Kwajalein Atoll in September 2016 and the archiving of the specimens at BISH validate the previous observation of this seagrass genus in the Marshall Islands. Previously, no voucher specimen was available for examination. Molecular analyses of the Kwajalein Halophila specimens may demonstrate conspecificity with Halophila nipponica J. Kuo with H. gaudichaudii relegated as a synonym. Herbarium specimens of Cymodocea rotundata Ehrenberg and Hemprich ex Ascherson from Majuro Atoll were found at BISH and may represent the only specimens from the Marshall Islands archived in a herbarium. Cymodocea rotundata, however, has been documented in past literature and archived via digital photos in its natural habitat in Majuro. The previous validation of Thalassia hemprichii (Ehrenberg) Ascherson with specimens, and the recent validation of Halophila gaudichaudii and Cymodocea rotundata with specimens reaffirm the low coral atolls and islands of the Marshall Islands as the eastern limit for the three species in the Pacific Ocean. Introduction In a review of the seagrasses in Micronesia, Tsuda et al. (1977) reported nine species of seagrasses in Micronesia with new records of Thalassodendron ciliatum (Forsskål) den Hartog from Palau, and Syringodium isoetifolium (Ascherson) Dandy and Cymodocea serrulata (R.
    [Show full text]
  • Seagrasses from the Philippines
    SMITHSONIAN CONTRIBUTIONS TO THE MARINE SCIENCES •NUMBER 21 Seagrasses from the Philippines Ernani G. Mefiez, Ronald C. Phillips, and Hilconida P. Calumpong ISSUED DEC 11983 SMITHSONIAN PUBLICATIONS SMITHSONIAN INSTITUTION PRESS City of Washington 1983 ABSTRACT Menez, Ernani G., Ronald C. Phillips, and Hilconida P. Calumpong. Sea­ grasses from the Philippines. Smithsonian Contributions to the Marine Sciences, number 21, 40 pages, 26 figures, 1983.—Seagrasses were collected from various islands in the Philippines during 1978-1982. A total of 12 species in seven genera are recorded. Generic and specific keys, based on vegetative characters, are provided for easier differentiation of the seagrasses. General discussions of seagrass biology, ecology, collection and preservation are presented. Local and world distribution of Philippine seagrasses are also included. OFFICIAL PUBLICATION DATE is handstamped in a limited number of initial copies and is recorded in the Institution's annual report, Smithsonian Year. SERIES COVER DESIGN: Seascape along the Atlantic coast of eastern North America. Library of Congress Cataloging in Publication Data Menez, Ernani G. Seagrasses from the Philippines. (Smithsonian contributions to the marine sciences ; no. 21) Bibliography: p. Supt. of Docs, no.: SI 1.41:21 1. Seagrasses—Philippines. I. Phillipps, Ronald C. II. Calumpong, Hilconida P. III. Ti­ tle. IV. Series. QK495.A14M46 1983 584.73 83-600168 Contents Page Introduction 1 Acknowledgments 3 Materials and Methods 3 Collecting and Preserving Seagrasses 4 General Features of Seagrass Biology and Ecology 6 Key to the Philippine Seagrasses 7 Division ANTHOPHYTA 8 Class MONOCOTYLEDONEAE 8 Order HELOBIAE 8 Family POTAMOGETONACEAE 8 Cymodocea rotundata Ehrenberg and Hemprich, ex Ascherson 8 Cymodocea serrulata (R.
    [Show full text]
  • Of the Seagrass Thalassodendron Ciliatum (Cymodoceaceae) Along the East African Coast
    Leaf production, shoot demography, and flowering frequency of the seagrass Thalassodendron ciliatum (Cymodoceaceae) along the East African coast Item Type Report Section Authors Kamermans, Pauline; Hemminga, Marten; Marba, Nuria; Mateo, Miguel A.; Mtolera, Matern; Stapel, Johan Publisher Netherlands Institute of Ecology, Centre for Esturarine and Coastal Ecology Download date 04/10/2021 00:42:22 Link to Item http://hdl.handle.net/1834/8444 Leaf production, shoot demography, and flowering frequency of the seagrass Thalassodendron ciliatum (Cymodoceaceae) along the East African coast Pauline Kamermans1*, Marten A. Hemminga1, Nuria Marbd1, Miguel A. Mateo1, Matern Mtolera2 & Johan Stapel1 1 Netherlands Institute of Ecology, Centre for Estuarine and Coastal Ecology, P.O. Box 140, 4400 AC Yerseke, The Netherlands 2 Institute of Marine Sciences, University of Dar es Salaam, P.O. Box 668, Zanzibar, Tanzania 'corresponding author: tel. +31-113-577452, fax. +31-113-573616, email [email protected] Several characteristics of Thalassondendron ciliatum populations were evaluated by a large scale sampling effort along the Kenyan coast and Zanzibar Island, with the aim to study spatial variability. A reconstruction technique, which makes use of scars left by abscised leaves and flowers, was employed to determine leaf production, shoot demography and flowering frequency of a number of T. ciliatum populations. Eight subtidal back-reef lagoons were sampled. Furthermore, samples were collected in an exposed subtidal site, intertidal rock pools, and a subtidal mangrove bay. Leaf production rates ranged from 33 to 57 leaves shoot'1 year1. Differences could not be related to habitat type. Median ages of the populations varied almost six fold from 0.34 year to 1.93 years.
    [Show full text]
  • Egyptian Red Sea Seagrass As a Source of Biologically Active Secondary Metabolites Abdel-Hamid A
    [Downloaded free from http://www.epj.eg.net on Tuesday, January 26, 2021, IP: 158.232.3.16] 224 Original Article Egyptian red sea seagrass as a source of biologically active secondary metabolites Abdel-Hamid A. Hamdya, Nabaweya M. El-Fikyb, Ahmed A. El-Beiha, Magdy M.D. Mohammedc, Walaa S.A. Mettwallya aDepartment of Chemistry of Natural and Background and objective Microbial Products National Research Centre, The Red Sea seagrass Halophila stipulacea (Forsskål) Ascherson and Dokki, Giza, bDepartment of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Thalassodendron ciliatum (Forsskål) den Hartog were poorly investigated either cDepartment of Pharmacognosy, National for their biological activities or for chemical constituents. This study aims to Research Centre, Dokki, Giza, Egypt investigate the phytochemical constituent of both grass, along with studying the Correspondence to Walaa S.A. Mettwally, PhD, different biological activities (osteoclastogenesis, antioxidant activity, and Department of Chemistry of Natural and anticancer activity) of the crude extract as well as purified compounds. Microbial Products, National Research Centre, Materials and methods 12311 Dokki, Giza, Egypt. The present study used three different in-vitro bioassay methods to screen the Tel: +20 238 339 394; fax: +20 3337 0931; fractions and/or isolated compounds of both seagrass, to assess their possible e-mail: [email protected] biological activity. Osteoclastogenesis assay, antioxidant activity, and anticancer Received: 15 November 2019 activity were carried out using different assays of the different anticancer Revised: 29 February 2020 mechanism of action. Accepted: 15 March 2020 Published: 30 September 2020 Results and conclusion Ten secondary metabolites were isolated and identified for the first time from Red Egyptian Pharmaceutical Journal 2020, 19:224–237 Sea seagrass H.
    [Show full text]
  • Of the Red Sea
    SeagrassesField Guide to of the Red Sea By Amgad El Shaffai Edited by: Anthony Rouphael, PhD Ameer Abdulla, PhD SeagrassesField Guide to of the Red Sea Text and Photographs by Amgad El Shaffai Edited by: Anthony Rouphael, PhD Ameer Abdulla, PhD The designation of geographical entities in this book, and the presentation of the material, do not imply the expression of any opinion whatsoever on the part of IUCN, concerning the legal status of any country, territory, or area, or of its authorities, or concerning the delimitation of its frontiers or boundaries. The views expressed in this publication do not necessarily reflect those of IUCN. Published by: IUCN, Gland, Switzerland and Total Foundation, Courbevoie, France. Copyright: © 2011 International Union for Conservation of Nature and Natural Resources Reproduction of this publication for educational or other noncommercial purposes is authorized without prior written permission from the copyright holder provided the source is fully acknowledged. Reproduction of this publication for resale or other commercial purposes is prohibited without prior written permission of the copyright holder. Citation: El Shaffai, A. (2011). Field Guide to Seagrasses of the Red Sea. Rouphael, A. and Abdulla, A., eds. First Edition. Gland, Switzerland: IUCN and Courbevoie, France: Total Foundation. viii + 56pp. ISBN: 978-2-8317-1414-1 This publication is a contribution of the Marine Biodiversity and Conservation Science Group. Design and layout: Tasamim Design - www.tasamim.net Cover picture: Amgad El Shaffai All photographs used in this publication remain the property of the original copyright holder. Photographs should not be reproduced or used in other contexts without written permission from the copyright holder.
    [Show full text]
  • Seagrass Syllabus a Training Manual for Resource Managers
    SEAGRASS SYLLABUS A TRAINING MANUAL FOR RESOURCE MANAGERS WORLD SEAGRASS ASSOCIATION Authors: Giuseppe Di Carlo, Len McKenzie Contributing authors: Leanne Cullen, Cleto Nanola, Miguel Fortes, Richard Unsworth, W. Jud Kenworthy Design and layout: Catherine Roberts Water color illustrations: Ruth Berry, copyright Fisheries Queensland (DEEDI) This manual was produced by the World Seagrass Association for Conservation International, with the generous support of the Flora Family Foundation. This publication should be cited as: Di Carlo G., McKenzie L.J. 2011. Seagrass training manual for resource managers. Conservation International, USA. Conservation International 2011 Crystal Drive, Suite 500 Arlington, VA 22202 USA Web: www.conservation.org For more information on the seagrass trainings please contact the World Seagrass Association at [email protected] or visit www.worldseagrass.org CONTENTS 5 INTRODUCTION Purpose of this manual Who is this manual for? General introduction to seagrasses Global trends in seagrass ecosystems Seagrasses and Marine Protected Areas Learning objectives 7 MODULE 1: WHAT ARE SEAGRASSES? BASIC NOTIONS OF THEIR BIOLOGY AND ECOLOGY Module Objectives What are seagrasses? Where are seagrasses found? The seagrass plant What keeps seagrass healthy? The ecological role of seagrass beds Activity: seagrass morphology17 (!lling in the gaps) 12 MODULE 2: KEY SEAGRASS SPECIES AND HOW TO IDENTIFY THEM Module Objectives Species diversity Classi!cation of seagrass species Key distinguishing features Activity: Seagrass Identi!cation
    [Show full text]
  • Diversity and Distribution of Seagrasses Around Inhaca Island, Southern Mozambique
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector South African Journal of Botany 2002, 68: 191–198 Copyright © NISC Pty Ltd Printed in South Africa — All rights reserved SOUTH AFRICAN JOURNAL OF BOTANY ISSN 0254–6299 Diversity and distribution of seagrasses around Inhaca Island, southern Mozambique SO Bandeira Department of Marine Botany, Göteborg University, PO Box 461, SE 405 30 Göteborg, Sweden Present address: Department of Biological Sciences, Universidade Eduardo Mondlane, PO Box 257, Maputo, Mozambique e-mail: [email protected] Received 21 September 2000, accepted in revised form 15 August 2001 Nine seagrass species were identified around Inhaca as tion key is presented. Seagrasses covered half of the well as a more narrow form of Thalassodendron cilia- whole intertidal area around Inhaca Island and diversity tum (Forsk.) den Hartog occurring in rocky pools at the was also high at community level. Transects at macro north east coast. Seagrasses were mapped and and micro scales showed zonation of species which grouped in seven distinct community types (in order of may be due to tidal gradients or topographic variation. areas covered): Thalassia hemprichii/Halodule wrightii, Cluster analysis indicated ecological dissimilarities Zostera capensis, Thalassodendron ciliatum/Cymodocea between co-dominant species. A decline of Zostera serrulata, Thalassodendron ciliatum/seaweeds, capensis has recently been seen outside the local vil- Cymodocea rotundata/Halodule wrightii, Cymodocea lage. Baseline studies like this are important for coastal serrulata and Halophila ovalis /Halodule wrightii; with zone management in developing countries, where large the ninth species Syringodium isoetifolium occurring in future changes in seagrass cover can be expected.
    [Show full text]
  • Review Article Highlights in Seagrasses' Phylogeny, Physiology
    International Scholarly Research Network ISRN Botany Volume 2012, Article ID 103892, 15 pages doi:10.5402/2012/103892 Review Article Highlights in Seagrasses’ Phylogeny, Physiology, and Metabolism: What Makes Them Special? Jutta Papenbrock Institute of Botany, Leibniz University Hannover, Herrenhauser¨ Straße 2, 30419 Hannover, Germany Correspondence should be addressed to Jutta Papenbrock, [email protected] Received 1 October 2012; Accepted 20 November 2012 Academic Editors: M. Kwaaitaal and I. Zarra Copyright © 2012 Jutta Papenbrock. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The marine seagrasses form an ecological and therefore paraphyletic group of marine hydrophilus angiosperms which evolved three to four times from land plants towards an aquatic and marine existence. Their taxonomy is not yet solved on the species level and below due to their reduced morphology. So far also molecular data did not completely solve the phylogenetic relationships. Thus, this group challenges a new definition for what a species is. Also their physiology is not well understood due to difficult experimental in situ and in vitro conditions. There remain several open questions concerning how seagrasses adapted secondarily to the marine environment. Here probably exciting adaptation solutions will be detected. Physiological adaptations seem to be more important than morphological ones. Seagrasses contain several compounds in their secondary metabolism in which they differ from terrestrial plants and also not known from other taxonomic groups. Some of these compounds might be of interest for commercial purposes.
    [Show full text]
  • Seagrasses of the Great Barrier Reef JANET LANYON
    Seagrasses of the Great Barrier Reef JANET LANYON IIE.strallorls Geo~f Kelly ~.~.~,.Great Barrier Ree| Marine Park Authority ~'Special PubJication Series (3) Guide to the Identification of Seagrasses in the Great Barrier Reef Region JANET LANYON Illustrations Geoff Kelly ~ Great Barrier Reef Marine Park Authority Special Publication Series (3) ISSN 0810--6983 ISBN 0--642--52489--0 Cover Design: Geoff Kelly NOTES ABOUT THE AUTHOR Janet Lanyon is currently doing a PhD in Zoology with supervisors at both Monash and James Cook Universities. Her interest in seagrass stems from research in which she is examining seagrass as food for dugongs. In addition to monitoring seasonal changes in the seagrass meadows of the Townsville region, Janet has been involved in seagrass surveys within the Great Barrier Reef Marine Park and Torres Strait. Her other interests include nutritional biology and ecology, particularly of Australian mammals, plant-herbivore relationships, marine mammal biology and various aspects of reef ecology. Published by GBRMPA 1986 Typesetting and artwork: Tim Weaver Graphics, Townsville, Queensland. Printed by Nadicprint Services Pty. Ltd., Townsville, Queensland. ACKNOWLEDGEMENTS I wish to thank the following people for their valuable comments and criticism of the manuscript: Dr. Helene Marsh, Dr. Gordon Sanson, Mark Simmons, Dr. lan Price, Dr. Tony Larkum, Robert Coles, Dr Peter Arnold and Ruth Lanyon. Thanks must also go to Bey and Lyle Squire and Warren Lee Long of Queensland Fisheries, Cairns, for providing additional seagrass specimens, and to the seagrass workshop participants who attempted and commented on the taxonomic keys. I am most grateful to both Dr. lan Poiner of CSIRO Marine Laboratories, and the Great Barrier Reef Marine Park Authority for allowing me to participate in recent northern Australian seagrass surveys.
    [Show full text]
  • Highlights in Seagrasses' Phylogeny, Physiology, and Metabolism: What
    International Scholarly Research Network ISRN Botany Volume 2012, Article ID 103892, 15 pages doi:10.5402/2012/103892 Review Article Highlights in Seagrasses’ Phylogeny, Physiology, and Metabolism: What Makes Them Special? Jutta Papenbrock Institute of Botany, Leibniz University Hannover, Herrenhauser¨ Straße 2, 30419 Hannover, Germany Correspondence should be addressed to Jutta Papenbrock, [email protected] Received 1 October 2012; Accepted 20 November 2012 Academic Editors: M. Kwaaitaal and I. Zarra Copyright © 2012 Jutta Papenbrock. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The marine seagrasses form an ecological and therefore paraphyletic group of marine hydrophilus angiosperms which evolved three to four times from land plants towards an aquatic and marine existence. Their taxonomy is not yet solved on the species level and below due to their reduced morphology. So far also molecular data did not completely solve the phylogenetic relationships. Thus, this group challenges a new definition for what a species is. Also their physiology is not well understood due to difficult experimental in situ and in vitro conditions. There remain several open questions concerning how seagrasses adapted secondarily to the marine environment. Here probably exciting adaptation solutions will be detected. Physiological adaptations seem to be more important than morphological ones. Seagrasses contain several compounds in their secondary metabolism in which they differ from terrestrial plants and also not known from other taxonomic groups. Some of these compounds might be of interest for commercial purposes.
    [Show full text]
  • New Perspectives on Western Australian Seagrass and Macroalgal Biogeography
    Journal of the Royal Society of Western Australia, 103: 29–37, 2020 New perspectives on Western Australian seagrass and macroalgal biogeography DIANA WALKER 1 *, JOHN M HUISMAN 2, KIERYN KILMINSTER 1,3 & JOHN KUO 4 1 School of Biological Sciences, University of Western Australia, 35 Stirling Highway, Perth, WA 6009, Australia 2 Western Australian Herbarium, Biodiversity and Conservation Science, Department of Biodiversity, Conservation and Attractions, 17 Dick Perry Avenue Kensington, Locked Bag 104, Bentley Delivery Centre, WA 6983, Australia 3 Department of Water and Environmental Regulation, WA Government, Locked Bag 10 Joondalup DC, WA 6919, Australia 4 Research Infrastructure Centres, University of Western Australia, 35 Stirling Highway, Perth, WA 6009, Australia * Corresponding author: [email protected] Abstract The widespread adoption of new methodologies, especially molecular techniques, has dramatically changed our understanding of how species of seagrasses and macroalgae are classified and distributed. One consequence of this new paradigm is increased uncertainty regarding biogeographic studies based on pre-molecular species records. The question “how does one delineate an individual species?” has changed and differing interpretations may alter how previous assessments are viewed. In some instances, specimens previously regarded as a single species have been shown to represent multiple genetic lineages. An extreme example is that of the red alga Portiera hornemannii, now thought to include 21 cryptic species in the Philippines alone, and possibly up to 96 species in the wider Indo-Pacific. Reworking and reclassification of species based on DNA analyses have sunk many species into one, or combined or reorganized genera. These changes in taxonomic concepts have implications for conservation and biogeographical assessments, but our understanding of many groups is still in its infancy and requires further work.
    [Show full text]