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Setophoma Spp. on Camellia Sinensis

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VOLUME 4 DECEMBER 2019 Fungal Systematics and Evolution PAGES 43–57 doi.org/10.3114/fuse.2019.04.05 Setophoma spp. on Camellia sinensis F. Liu1, J. Wang1,2, H. Li3, W. Wang4, L. Cai1, 2* 1State key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China 2College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China 3College of Life Sciences, Hebei University, Baoding, Hebei Province, 071002, China 4Shandong Hetian Wang Biological Technology Co., Ltd., WeiFang, 261300, China *Corresponding author: [email protected] Key words: Abstract: During our investigation of Camellia sinensis diseases (2013–2018), a new leaf spot disease was found in seven five new taxa provinces of China (Anhui, Fujian, Guangxi, Guizhou, Jiangxi, Tibet and Yunnan), occurring on both arboreal and terraced fungal pathogen tea plants. The leaf spots were round to irregular, brown to dark brown, with grey or tangerine margins. Multi-locus (LSU, phylogeny ITS, gapdh, tef-1α, tub2) phylogenetic analyses combined with morphological observations revealed four new species taxonomy belonging to the genus Setophoma, i.e. S. antiqua, S. longinqua, S. yingyisheniae and S. yunnanensis. Of these four species, tea plants S. yingyisheniae was found to be present on diseased terraced tea plants in six of the seven sampled provinces (excluding Yunnan). The other three species only occurred on arboreal tea plants in Yunnan Province. In addition to the four species isolated from diseased leaves, S. endophytica sp. nov. was isolated from healthy leaves of terraced tea plants. Effectively published online: 15 May 2019. INTRODUCTION morphological comparison, host association and geographical Editor-in-Chief Prof. dr P.W. Crous, Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD Utrecht, The Netherlands. distribution. E-mail: [email protected] During our investigation of diseases of tea plants (Camellia sinensis) cultivated in China in 2013, a new leaf spot disease was found to cause severe yield losses in Guangxi Province. The MATERIALS AND METHODS associated leaf spots were circular to irregular, brown to dark brown in colour, with grey or tangerine margins. A subsequent Isolates collection effort of similarly affected tea plant leaves has been ongoing in many tea plantations located in six provinces of China, Isolates were obtained from either diseased or healthy tea i.e. Anhui, Fujian, Guizhou, Jiangxi, Tibet and Yunnan. Preliminary plant tissues collected from 18 locations in seven provinces of morphological observations and molecular analyses identified China, following the single spore isolation and tissue isolation the associated fungi as Setophoma spp. To our knowledge, this methods described in Liu et al. (2015). Type specimens of new is the first report of Setophoma on tea plants. Considering the species were deposited in the Mycological Herbarium of the potential commercial yield losses in tea plantations and the Institute of Microbiology, Chinese Academy of Sciences, Beijing, limited knowledge of this disease, accurate identification of the China (HMAS), with the ex-type living cultures being deposited causal organisms is of great importance. in the China General Microbiological Culture Collection Center The genus Setophoma (Phaeosphaeriaceae) was introduced (CGMCC). to accommodate Phoma terrestris and Pyrenochaeta sacchari (de Gruyter et al. 2010). Species of Setophoma are characterised DNA extraction, PCR amplification and phylogenetic as having setose pycnidia, phialidic conidiogenous cells and analyses hyaline, ellipsoidal to subcylindrical, aseptate conidia (de Gruyter et al. 2010, Quaedvlieg et al. 2013). According to Index Total genomic DNA was extracted from fresh mycelia using Fungorum and MycoBank, four additional Setophoma species the CTAB method. Five partial loci including the large subunit have been described since the genus was introduced in 2010. of the nrDNA (LSU), the 5.8S nuclear ribosomal RNA gene with The currently recognised species are: S. chromolaenae, S. the two flanking internally transcribed spacer regions (ITS), cyperi, S. poaceicola, S. sacchari, S. terrestris, and S. vernoniae. partial glyceraldehyde-3-phosphate dehydrogenase (gapdh), All except S. terrestris are reported to occur on unique host translation elongation factor 1-alpha (tef-1α) and β-tubulin plants (Table 1). (tub2) were amplified and sequenced using the following primer The aim of the present study was to investigate the taxonomic pairs: ITS1/ITS4 for ITS (White et al. 1990), LR0R/LR5 for LSU and phylogenetic relationships of Setophoma spp. associated (Vilgalys & Hester 1990, Rehner & Samuels 1994), gpd1/gpd2 for with tea plants based on multi-locus phylogenetic analyses, gapdh (Berbee et al. 1999), EF-1/EF-2 for tef-1α (O’Donnell et al. Fungal Systematics and Evolution is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License © 2019 Westerdijk Fungal Biodiversity Institute 43 Liu et al. Table 1. Host and distribution of Setophoma species. Species Host Distribution References S. antiqua Camellia sinensis China This study S. chromolaenae Chromolaena odorata Brazil Quaedvlieg et al. (2013) S. cyperi Cyperus sphaerocephalus South Africa, Eastern Cape Crous et al. (2016) S. endophytica Camellia sinensis China This study S. longinqua Camellia sinensis Editor-in-Chief China This study Prof. dr P.W. Crous, Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD Utrecht, The Netherlands. E-mail: [email protected] S. poaceicola Grass Thailand Thambugala et al. (2017) S. sacchari Saccharum officinarum Brazil de Gruyter et al. (2010) S. terrestris Allium cepa North America, Senegal de Gruyter et al. (2010) Allium sativum United States de Gruyter et al. (2010) Brassica sp. Canada, Alberta Yang et al. (2017) Cucurbita maxima USA, Oregon Rivedal et al. (2018) Cucurbita moschata Japan Ikeda et al. (2012) Solanum lycopersicum Canada, Ontario Johnston-Monje et al. (2017) S. vernoniae Vernonia polyanthes Brazil Crous et al. (2014) S. yingyisheniae Camellia sinensis China This study S. yunnanensis Camellia sinensis China This study Table 2. GenBank accession numbers from NCBI database. Name Strain/specimen No.a LSU ITS Acericola italica MFLUCC 13-0609* MF167429 MF167428 Allophaeosphaeria muriformia MFLUCC 13-0349* KP765681 KP765680 Allophaeosphaeria subcylindrospora MFLUCC 13-0380* KT314183 KT314184 Amarenomyces ammophilae CBS 114595 GU301859 KF766146 Ampelomyces quisqualis CBS 129.79* EU754128 HQ108038 Bhatiellae rosae MFLUCC 17-0664* MG828989 MG828873 Chaetosphaeronema achilleae MFLUCC 16-0476 KX765266 KX765265 Chaetosphaeronema hispidulum CBS 216.75 KF251652 KF251148 Coniothyrium carteri CBS 105.91 KF251712 KF251209 Dactylidina dactylidis MFLUCC 14-0966* MG829002 MG828886 Dematiopleospora rosicola MFLU 16-0232* MG829006 MG828888 Dematiopleospora salsolae MFLUCC 17-0828* MG829007 MG828889 Didymocyrtis consimilis Gardiennet 12041 KT383796 KT383813 Didymocyrtis ramalinae Ertz 16399 KT383802 KT383838 Embarria clematidis MFLUCC 14-0976* MG828987 MG828871 Galiicola pseudophaeosphaeria MFLUCC 14-0524* KT326693 KT326692 Hawksworthiana alliariae MFLUCC 13-0070 KX494877 KX494876 Hawksworthiana clematidicola MFLUCC 14-0910* MG829011 MG828901 Italica achilleae MFLUCC 14-0959* MG829013 MG828903 Juncaceicola achilleae MFLUCC 13-0606* KX449526 KX449525 Juncaceicola luzulae MFLUCC 16-0780* KX449530 KX449529 Juncaceicola typharum CBS 296.54 KF251695 KF251192 Leptospora galii KUMCC 15-0521 KX599548 KX599547 Leptospora rubella CPC 11006 DQ195792 DQ195780 Muriphaeosphaeria galatellae MFLUCC 14-0614* KT438329 KT438333 Neosetophoma samarorum CBS 138.96* KF251664 KF251160 Neostagonospora caricis CBS 135092* KF251667 KF251163 Neostagonospora elegiae CBS 135101* KF251668 KF251164 44 © 2019 Westerdijk Fungal Biodiversity Institute Setophoma spp. on Camellia sinensis Table 2. (Continued). Name Strain/specimen No.a LSU ITS Neosulcatispora agaves CPC 26407* KT950867 KT950853 Nodulosphaeria hirta MFLUCC 13-0867 KU708845 KU708849 Nodulosphaeria scabiosae MFLUCC 14-1111* KU708846 KU708850 Ophiobolopsis italica MFLUCC 17-1791* MG520959 MG520939 Ophiobolus artemisiae MFLUCC 14-1156* KT315509 KT315508 Editor-in-Chief Prof. dr P.W. Crous, Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD Utrecht, The Netherlands. E-mail: [email protected] Ophiobolus disseminans MFLUCC 17-1787* MG520961 MG520941 Ophiosimulans tanaceti MFLUCC 14-0525 KU738891 KU738890 Paraophiobolus arundinis MFLUCC 17-1789* MG520965 MG520945 Paraophiobolus plantaginis MFLUCC 17-0245* KY815010 KY797641 Paraphoma chrysanthemicola CBS 172.70 KF251669 KF251165 Paraphoma radicina CBS 102875 KF251677 KF251173 Paraphoma rhaphiolepidis CBS 142524* KY979813 KY979758 Paraphoma vinacea UMPV001 = BRIP 63684 KU176888 KU176884 Parastagonospora caricis CBS 135671 KF251680 KF251176 Parastagonospora nodorum CBS 110109 KF251681 KF251177 Parastagonospora poagena CBS 136776* KJ869174 KJ869116 Parastagonosporella fallopiae CBS 135981* MH460545 MH460543 CCTU 1151.1 MH460546 MH460544 Phaeosphaeria oryzae CBS 110110* KF251689 KF251186 Phaeosphaeria papayae CBS 135416 KF251690 KF251187 Phaeosphaeriopsis glaucopunctata CBS 653.86 KF251702 KF251199 Poaceicola arundinis MFLU 15-0702* KU058726 KU058716 Poaceicola italica MFLUCC 13-0267* KX910094 KX926421 Populocrescentia forlicesenensis MFLU 15-0651* KT306952 KT306948 Pseudoophiobolus achilleae MFLU 17-0925* MG520966 MG520946 Pseudoophiobolus
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    Polish Botanical Journal 55(2): 359–371, 2010 CLADOSPORIUM EPICHLOËS, A RARE EUROPEAN FUNGUS, WITH NOTES ON OTHER FUNGICOLOUS SPECIES* MAŁGORZATA RUSZKIEWICZ-MICHALSKA Abstract. Cladosporium epichloës Lobik, associated with Epichloë typhina (Pers.) Tul. & C. Tul. and known from three records worldwide, is reported from Poland for the fi rst time. The morphology and distribution data of this species as well as the fi rst records of Cladosporium uredinicola Speg. and Phoma glomerata (Corda) Wollenw. & Hochapfel as parasites of powdery mil- dews in Poland are presented. Information concerning specimens of fi ve other hyperparasitic species deposited in Herbarium Universitatis Lodziensis (LOD) is provided. Key words: Cladosporium, Phoma glomerata, Sphaerellopsis fi lum, Ampelomyces, Tuberculina, hyperparasite, fungicolous fungi, chorology, Poland Małgorzata Ruszkiewicz-Michalska, Department of Mycology, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland; e-mail: [email protected] INTRODUCTION The term ‘fungicolous fungi’ covers species that spores via appresorium-like structures (Płachecka occur on other fungi as parasites, commensals or 2005 and literature cited therein). saprobionts (Kirk et al. 2008). The nature of the A reliable estimate of the number of fungi- interfungal relationships is not always clear, es- colous species is not available (Kirk et al. 2008). pecially for intimate mycoparasitic interactions Hawksworth (1981), however, traced 1100 an- (Jeffries & Young 1994). Opinions on the type of amorphic species recorded on other species of relations of particular species with their mycohosts fungi. Fungicolous fungi include members of all vary considerably. The status of the mycoparasite higher taxa of true fungi (Chytridiomycota, Zygo- Ampelomyces quisqualis Ces., considered to be mycota, Ascomycota including anamorphic taxa, an intracellular necrotroph vs.
  • Taxonomic and Phylogenetic Placement of Nodulosphaeria

    Taxonomic and Phylogenetic Placement of Nodulosphaeria

    Mycol Progress (2016) 15:34 DOI 10.1007/s11557-016-1176-x ORIGINAL ARTICLE Taxonomic and phylogenetic placement of Nodulosphaeria Ausana Mapook1,2,4,5 & Saranyaphat Boonmee 5 & Hiran A. Ariyawansa4,5,9 & Saowaluck Tibpromma4,5 & Erio Campesori6,7,8 & E. B. Gareth Jones3 & Ali H. Bahkali3 & K. D. Hyde1,2,3,5 Received: 27 August 2015 /Revised: 15 February 2016 /Accepted: 17 February 2016 # German Mycological Society and Springer-Verlag Berlin Heidelberg 2016 Abstract Nodulosphaeria is a ubiquitous genus that com- the family Phaeosphaeriaceae as a distinct genus. The sexual prises saprobic, endophytic and pathogenic species associated morphs of Nodulosphaeria hirta and N. spectabilis are de- with a wide variety of substrates and has 64 species epithets scribed and illustrated using modern concepts. Two new listed in Index Fungorum. The classification of species in the Nodulosphaeria species are introduced. The phylogenetic re- genus has been a major challenge due to a lack of understand- lationships and taxonomy of the genus Nodulosphaeria are ing of the importance of characters used to distinguish taxa, as discussed, but further sampling with fresh collections, refer- well as the lack of reference strains. The present study clarifies ence or ex-type strains and molecular data are needed to obtain the phylogenetic placement of the genus and related species, a better and natural classification for the genus. using fresh collections from Italy. Four Nodulosphaeria spe- cies are characterized based on multi-loci analyses of ITS, LSU, SSU, TEF and RPB2 sequence datasets. Phylogenetic Keywords Dothideomycetes . Phaeosphaeriaceae . analyses indicate that Nodulosphaeria species group within Phylogeny . Taxonomy Section Editor: Franz Oberwinkler * K.
  • Microfungi Associated with Camellia Sinensis: a Case Study of Leaf and Shoot Necrosis on Tea in Fujian, China

    Microfungi Associated with Camellia Sinensis: a Case Study of Leaf and Shoot Necrosis on Tea in Fujian, China

    Mycosphere 12(1): 430–518 (2021) www.mycosphere.org ISSN 2077 7019 Article Doi 10.5943/mycosphere/12/1/6 Microfungi associated with Camellia sinensis: A case study of leaf and shoot necrosis on Tea in Fujian, China Manawasinghe IS1,2,4, Jayawardena RS2, Li HL3, Zhou YY1, Zhang W1, Phillips AJL5, Wanasinghe DN6, Dissanayake AJ7, Li XH1, Li YH1, Hyde KD2,4 and Yan JY1* 1Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, People’s Republic of China 2Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Tha iland 3 Tea Research Institute, Fujian Academy of Agricultural Sciences, Fu’an 355015, People’s Republic of China 4Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People’s Republic of China 5Universidade de Lisboa, Faculdade de Ciências, Biosystems and Integrative Sciences Institute (BioISI), Campo Grande, 1749–016 Lisbon, Portugal 6 CAS, Key Laboratory for Plant Biodiversity and Biogeography of East Asia (KLPB), Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, Yunnan, People’s Republic of China 7School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, People’s Republic of China Manawasinghe IS, Jayawardena RS, Li HL, Zhou YY, Zhang W, Phillips AJL, Wanasinghe DN, Dissanayake AJ, Li XH, Li YH, Hyde KD, Yan JY 2021 – Microfungi associated with Camellia sinensis: A case study of leaf and shoot necrosis on Tea in Fujian, China. Mycosphere 12(1), 430– 518, Doi 10.5943/mycosphere/12/1/6 Abstract Camellia sinensis, commonly known as tea, is one of the most economically important crops in China.