Symposium As Severe As That Caused by the Serogroup O157 Strains
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
The Food Poisoning Toxins of Bacillus Cereus
toxins Review The Food Poisoning Toxins of Bacillus cereus Richard Dietrich 1,†, Nadja Jessberger 1,*,†, Monika Ehling-Schulz 2 , Erwin Märtlbauer 1 and Per Einar Granum 3 1 Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig Maximilian University of Munich, Schönleutnerstr. 8, 85764 Oberschleißheim, Germany; [email protected] (R.D.); [email protected] (E.M.) 2 Department of Pathobiology, Functional Microbiology, Institute of Microbiology, University of Veterinary Medicine Vienna, 1210 Vienna, Austria; [email protected] 3 Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, P.O. Box 5003 NMBU, 1432 Ås, Norway; [email protected] * Correspondence: [email protected] † These authors have contributed equally to this work. Abstract: Bacillus cereus is a ubiquitous soil bacterium responsible for two types of food-associated gastrointestinal diseases. While the emetic type, a food intoxication, manifests in nausea and vomiting, food infections with enteropathogenic strains cause diarrhea and abdominal pain. Causative toxins are the cyclic dodecadepsipeptide cereulide, and the proteinaceous enterotoxins hemolysin BL (Hbl), nonhemolytic enterotoxin (Nhe) and cytotoxin K (CytK), respectively. This review covers the current knowledge on distribution and genetic organization of the toxin genes, as well as mechanisms of enterotoxin gene regulation and toxin secretion. In this context, the exceptionally high variability of toxin production between single strains is highlighted. In addition, the mode of action of the pore-forming enterotoxins and their effect on target cells is described in detail. The main focus of this review are the two tripartite enterotoxin complexes Hbl and Nhe, but the latest findings on cereulide and CytK are also presented, as well as methods for toxin detection, and the contribution of further putative virulence factors to the diarrheal disease. -
The Eucharist Is the Body and Blood, Soul and Divinity of Jesus Christ
The Eucharist Is the Body and Blood, Soul and Divinity of Jesus Christ UNIT 7, LESSON 2 Learning Goals Connection to the Catechism ӹ The miracle of the multiplication of the Catholic Church of the loaves and the fish ӹ CCC 547-548 ӹ CCC 1392-1397 foreshadows the institution of ӹ CCC 1335-1336 ӹ CCC 1411-1412 the Eucharist at the Last Supper. ӹ CCC 1360 ӹ CCC 1415 ӹ Jesus instituted the Eucharist at the Last Supper as a memorial of ӹ CCC 1365 ӹ CCC 1417 His Death and Resurrection, and ӹ CCC 1373-1376 He commanded His Apostles to celebrate it until His return. Vocabulary At Mass, the bread and wine ӹ ӹ Eucharist ӹ Essential are changed into the Body and ӹ Chalice Elements of the Blood, Soul and Divinity of Jesus Eucharist Christ in the Holy Eucharist. ӹ Real Presence ӹ State of Grace ӹ The Eucharist is a sacrifice of ӹ Last Supper thanksgiving. BIBLICAL TOUCHSTONES While they were eating, Jesus took bread, said I am the bread of life; whoever comes to me will the blessing, broke it, and giving it to his disciples never hunger, and whoever believes in me will said, “Take and eat; this is my body.” Then he took never thirst. a cup, gave thanks, and gave it to them, saying, JOHN 6:35 “Drink from it, all of you, for this is my blood of the covenant, which will be shed on behalf of many for the forgiveness of sins.” MATTHEW 26:26-28 653 Lesson Plan Materials ӹ Feeding the Five Thousand ӹ Appendix A: My First ӹ The Real Presence Communion Journal ӹ The Last Supper and the Mass ӹ Crayons, markers, and/ or colored pencils ӹ The Eucharist ӹ Paper plates ӹ Teacher Resource: The Multiplication of the Loaves ӹ Scissors and the Fish Comic Strip ӹ Glue ӹ Teacher Resource: ӹ Blank paper Loaves and Fish ӹ Small tablecloth ӹ Teacher Resource: ӹ Flameless candle The Last Supper Prayer Soul of Christ, sanctify me. -
Blood in Hittite Ritual
Blood IN Hittite Ritual Gary Beckman (University of Michigan) The generally anonymous authors of the texts that describe the rites of the Hittite state cult were practical men.1 Laconically they set forth the procedures to be followed and wasted few words explicating or justifying either the ceremonies or the individual ritual actions of which they were composed. Thus in our consideration of the meaning and function of blood in Hittite religious practice,2 we cannot avail ourselves of any clear statement of na- tive belief analogous to that found for Israel in the Hebrew Bible: “For the life of the flesh is in the blood; and I have given it to you for making atonement for your lives on the altar; for, as life, it is the blood that makes atonement.”3 Nonetheless, Hittite compositions of various genres do contain passages revealing that in H ̮ atti blood (ešh ̮ar 4) was recognized as the carrier of life and strength. Conversely, its presence was taken as an index of mortality. In a legendary account of the campaigns conducted in Anatolia by the Sargonic king Narām-Sîn, the ruler commands that a scout be dispatched to perform the following test on terrifying beings encountered by his forces: When he proceeds to pierce(!) them with a spear and cut them with a blade(?)—if [blood] spurts forth from them, they are human, and I shall go against them (in battle). If blood does not spurt forth from them, they are deities, and I will not go against them.5 In the course of an “induction ceremony” into the Hittite army, wine is poured out on the ground before the sol- diers concerned, and the officiant addresses them: Abbreviations employed here are those of The Hittite Dictionary of the Oriental Institute of the University of Chicago (1980–). -
Identification of Mrna As Biomarker of Bacillus Weihenstephanensis Acid Resistance : Toward the Integration of Omic Data Into Predictive Microbiology Noemie Desriac
Identification of mRNA as biomarker of Bacillus weihenstephanensis acid resistance : toward the integration of Omic data into predictive microbiology Noemie Desriac To cite this version: Noemie Desriac. Identification of mRNA as biomarker of Bacillus weihenstephanensis acid resistance : toward the integration of Omic data into predictive microbiology. Microbiology and Parasitology. Université de Bretagne occidentale - Brest, 2013. English. NNT : 2013BRES0096. tel-02152918 HAL Id: tel-02152918 https://tel.archives-ouvertes.fr/tel-02152918 Submitted on 11 Jun 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. présentée par THÈSE / UNIVERSITÉ DE BRETAGNE OCCIDENTALE sous le sceau de l’Université européenne de Bretagne Noémie DESRIAC pour obtenir le titre de Préparée à ADRIA Développement et au DOCTEUR DE L’UNIVERSITÉ DE BRETAGNE OCCIDENTALE Laboratoire Universitaire de Biodiversité et Mention : Microbiologie Alimentaire d'Ecologie Microbienne École Doctorale SICMA Thèse soutenue le 04 Juillet 2013 Identification d’ARNm devant le jury composé de : -
IAFP Meeting
July 31-August 3 M Annual20 O N IAFPMeetingMilwaukee,11 WI D th All presentations to be held at Frontier Airlines Convention Center A Y MONDAY MORNING 9:15 Food Safety Activities in Thailand — PENSRI RODMA, AUGUST 1 Ministry of Public Health, Nonthaburi, Thailand A 10:00 Break M (Posters will be on display 10:00 a.m. − 6:00 p.m. 10:30 TBD — QINCY LISSAUR, BSI Standard Solutions, See details beginning on page 51.) London, United Kingdom S1 The Diverse and Discrepant Non-O157 STEC: 11:00 Moving Forward — Changing Cultures on Different Data, Differences and Discernment Levels — PATRICK WALL, University College Dublin, Frontier Airlines Convention Center, 203 Dublin, Ireland Sponsored by ILSI North America Technical 11:30 Panel Discussion Committee on Food Microbiology Organizer: Darinka Djordjevic S3 To Tell or Not to Tell, That is the Question! Convenor: Peter Gerner-Smidt Environmental Testing and Mandatory 8:30 The Increasing Importance of Non-O157 STEC in the Reporting — An Industry, Regulatory and United States — RAJAL MODY, Centers for Disease Public Relations Nightmare Control and Prevention, Atlanta, GA, USA Frontier Airlines Convention Center, 201AB 9:00 STEC Pathobiology and Virulence — LOTHAR BEUTIN, Sponsored by the IAFP Foundation Federal Institute for Risk Assessment, Berlin, Germany Organizers: Rudy Westervelt and Nancy Eggink 9:30 STEC Detection/Characterization Current Status — Convenors: Rudy Westervelt and Nancy Eggink Future Prospects — NANCY STROCKBINE, Centers for 8:30 Liability and Responsibility — The Legal Ramifications Disease Control and Prevention, Atlanta, GA, USA of Reporting or Not Reporting — SHAWN K. 10:00 Break STEVENS, Gass Weber Mullins, Milwaukee, WI, USA 10:30 Industry Perspectives on Non-O157 — TIMOTHY A. -
Improving the Iron Status of Children in Kisumu County Kenya Using Porridge Flour Enriched with Bovine Blood
IMPROVING THE IRON STATUS OF CHILDREN IN KISUMU COUNTY KENYA USING PORRIDGE FLOUR ENRICHED WITH BOVINE BLOOD Angela Adhiambo Andago BSc (Nagpur University) PGDE (Kenyatta University) MSc (University of Nairobi) A THESIS SUBMITTED IN FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY IN APPLIED HUMAN NUTRITION OF THE UNIVERSITY OF NAIROBI DEPARTMENT OF FOOD SCIENCE, NUTRITION AND TECHNOLOGY 2015 1 DECLARATION This thesis is my original work and has not been presented for a degree in any other university ____________________________________________________________________ This thesis has been submitted for examination with our approval as university supervisors. ____________________________________________ ___________________ Prof. Jasper K. Imungi. Date Department of Food Science, Nutrition and Technology ____________________________________________ ___________________ Dr. Alice M. Mwangi Date Department of Food Science, Nutrition and Technology. ____________________________________________ ___________________ Prof. Ruth W. Nduati Date Department of Paediatrics and Child Health i UNIVERSITY OF NAIROBI COLLEGE OF AGRICULTURE AND VETERINARY SCIENCES (CAVS) Faculty of Agriculture DEPARTMENT OF FOOD SCIENCE NUTRITION AND TECHNOLOGY (DFSNT) Plagiarism Declaration Form for Students Name of Student ___________________________________________________________ Registration Number ________________________________________________________ College ___________________________________________________________________ Faculty/School/Institute -
Assesment and Control of Bacillus Cereus Emetic Toxin in Food
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Helsingin yliopiston digitaalinen arkisto Assessment and control of Bacillus cereus emetic toxin in food Elina Jääskeläinen Department of Applied Chemistry and Microbiology Division Microbiology University of Helsinki Academic dissertation in Microbiology To be presented, with the permission of the Faculty of Agriculture and Forestry of the University of Helsinki, for public criticism in Auditorium 2041 at Viikki Biocenter, Viikinkaari 5, on February 1th, 2008, at 12 o´clock noon Helsinki 2008 Supervisor: Prof. Mirja S. Salkinoja-Salonen Department of Applied Chemistry and Microbiology Faculty of Agriculture and Forestry University of Helsinki Helsinki, Finland Reviewers: Prof. Willem M. de Vos 1) Laboratory of Microbiology Agrotechnology and Food Sciences Group Wageningen University and Research Centre Wageningen, the Netherlands 2) Department of Basic Veterinary Sciences Faculty of Veterinary Medicine University of Helsinki Helsinki, Finland Dr. Christophe Nguyen-The Institute of Plant Products Technology French National Institute for Agricultural Research (INRA) University of Avignon Avignon, France Opponent: Prof. Jacques Mahillon Laboratory of Food and Environmental Microbiology Université Catholique de Louvain Louvaine-la-Neuve, Belgium ISNN 1795-7079 ISBN 978-952-10-4458-8 (hardback) ISBN 978-952-10-4459-5 (PDF) Yliopistopaino Helsinki, Finland 2008 Front cover: Boys evaluating Mother`s art of cooking To my family Contents: List of original -
Cereulide and Valinomycin, Two Important Natural Dodecadepsipeptides with Ionophoretic Activities
Polish Journal of Microbiology 2010, Vol. 59, No 1, 310 MINIREVIEW Cereulide and Valinomycin, Two Important Natural Dodecadepsipeptides with Ionophoretic Activities MAGDALENA ANNA KROTEÑ, MAREK BARTOSZEWICZ* and IZABELA WIÊCICKA Department of Microbiology, Institute of Biology, University of Bia³ystok, wierkowa 20B, 15-950 Bia³ystok, Poland Received 2 July 2009, revised 20 December 2009, accepted 5 January 2010 Abstract Cereulide produced by Bacillus cereus sensu stricto and valinomycin synthesized mainly by Streptomyces spp. are natural dodecadepsipeptide ionophores that act as potassium transporters. Moreover, they comprise three repetitions of similar tetrapeptide motifs synthesized by non- ribosomal peptide synthesis complexes. Resemblances in their structure find their reflections in the same way of action. The toxicity of valinomycin and cereulide is an effect of the disturbance of ionic equilibrium and transmembrane potential that may influence the whole organism and then cause fatal consequences. The vlm and ces operons encoding valinomycin and cereulide are both composed of two large, similar synthetase genes, one thioestrase gene and four other ORFs with unknown activities. In spite of the characterization of valinomycin and cereulide, genetic determinants encoding their biosynthesis have not yet been clarified. Key words: Bacillus cereus, cereulide, valinomycin, ionophore Introduction lar hydrolytic enzymes and at least two third of known antibiotics (Omura et al., 2001). While cereulide, also The life and growth of both prokaryotic -
Detection and Isolation of Emetic Bacillus Cereus Toxin Cereulide by Reversed Phase Chromatography
toxins Communication Detection and Isolation of Emetic Bacillus cereus Toxin Cereulide by Reversed Phase Chromatography Eva Maria Kalbhenn 1, Tobias Bauer 1, Timo D. Stark 2 , Mandy Knüpfer 3, Gregor Grass 3 and Monika Ehling-Schulz 1,* 1 Functional Microbiology, Institute of Microbiology, Department of Pathobiology, University of Veterinary Medicine Vienna, 1210 Vienna, Austria; [email protected] (E.M.K.); [email protected] (T.B.) 2 Chair of Food Chemistry and Molecular Sensory Science, Technical University of Munich, Lise-Meitner-Straße 34, 85354 Freising, Germany; [email protected] 3 Bundeswehr Institute of Microbiology, Neuherbergstraße 11, 80937 Munich, Germany; [email protected] (M.K.); [email protected] (G.G.) * Correspondence: [email protected] Abstract: The emetic toxin cereulide is a 1.2 kDa dodecadepsipeptide produced by the food pathogen Bacillus cereus. As cereulide poses a serious health risk to humans, sensitive and specific detection, as well as toxin purification and quantification, methods are of utmost importance. Recently, a stable isotope dilution assay tandem mass spectrometry (SIDA–MS/MS)-based method has been described, and an method for the quantitation of cereulide in foods was established by the International Organization for Standardization (ISO). However, although this SIDA–MS/MS method is highly accurate, the sophisticated high-end MS equipment required for such measurements limits the method’s suitability for microbiological and molecular research. Thus, we aimed to develop a method for cereulide toxin detection and isolation using equipment commonly available in microbiological and biochemical research laboratories. Reproducible detection and relative quantification of cereulide Citation: Kalbhenn, E.M.; Bauer, T.; was achieved, employing reversed phase chromatography (RPC). -
Intinction: an Historical, Exegetical, and Systematic-Theological Examination Reverend Lane B
Intinction: An Historical, Exegetical, and Systematic-Theological Examination Reverend Lane B. Keister December 2012 Introduction: What Is At Stake? The Reformers commonly spoke of three marks of the true church: the gospel faithfully preached, the sacraments faithfully administered, and church discipline properly carried out. Intinction, which may be defined as the practice of dipping the bread into the wine during the administration of the Lord's Supper, affects one of the three marks of the church, i.e., the correct administration of the sacraments. No one in the debate would claim that the gospel is at stake. Nor would anyone claim that the Lord's Supper becomes null and void through the use of intinction. What this paper will attempt to prove is that the issue at stake is neither more nor less than the clarity of the sign of the Lord's Supper. As such, it is an issue that cannot be ignored. However, no church or teaching elder that currently practices intinction in our denomination should feel that they are under attack because of this practice. No church should be run out of town on a rail on this basis! If proponents of the change to the PCA's BCO should be victorious, all that would be required is a change in practice. These comments are offered for two reasons: 1. The temperature of the debate should be low. There is no need for heat in what should be a collegial and brotherly debate. 2. The issue needs to be seen in its proper context, as neither a gospel- level issue (a hill on which to die), nor an issue to be ignored (as if it were an attempt to prescribe, say, the shape of every Reformed church building). -
5, Webisode 10
Please note: Each segment in this Webisode has its own Teaching Guide The muckrakers had a good friend in Sam McClure. He founded McClure’s Magazine, which set a new standard for activist journalism and created a new field, investigative journalism. Most important, he hired the best writers he could find: Ida Tarbell, Lincoln Steffens, Jack London, Booth Tarkington, Rudyard Kipling, Stephen Crane, and Willa Cather. Ida Tarbell broke new ground not only in showing what a woman could do in a traditionally male occupation but also in setting a standard for scholarship, fairness, and integrity in the new field of investigative journalism. Her painstakingly researched The History of the Standard Oil Company detailed the illegal tactics used by John D. Rockefeller and led to the 1911 Supreme Court decision to break up the Standard Oil trust. Another female muckraker, Elizabeth Jane Cochrane, got her first job at a newspaper at age nineteen; by twenty-five, she was the most famous woman in the world, known as the daring round-the-world reporter Nellie Bly. Editor Sam McClure and energetic journalists exercised their First Amendment right and used the pen to expose the excesses of the Gilded Age. They gave birth to the field of investigative journalism. Teacher Directions 1. Ask students to predict. • From what you know about the last half of the nineteenth century, what problems might newspaper writers expose? 2. Allow time for student response. 3. Make sure students understand the following points in discussing the question. In the Gilded Age, industry boomed and large corporations grew. -
The Potential of Animal By-Products in Food Systems: Production, Prospects and Challenges
sustainability Review The Potential of Animal By-Products in Food Systems: Production, Prospects and Challenges Babatunde O. Alao 1,*, Andrew B. Falowo 1, Amanda Chulayo 1,2 and Voster Muchenje 1 1 Department of Livestock and Pasture Science, University of Fort Hare, Private Bag X314, Alice 5700, South Africa; [email protected] (A.B.F.); [email protected] (A.C.); [email protected] (V.M.) 2 Dohne Agricultural Development Institute, Department of Rural Development and Agrarian Reform, Private Bag X15, Stutterheim 4935, South Africa * Correspondence: [email protected]; Tel.: +27-833-46-4435 Received: 9 May 2017; Accepted: 16 June 2017; Published: 22 June 2017 Abstract: The consumption of animal by-products has continued to witness tremendous growth over the last decade. This is due to its potential to combat protein malnutrition and food insecurity in many countries. Shortly after slaughter, animal by-products are separated into edible or inedible parts. The edible part accounts for 55% of the production while the remaining part is regarded as inedible by-products (IEBPs). These IEBPs can be re-processed into sustainable products for agricultural and industrial uses. The efficient utilization of animal by-products can alleviate the prevailing cost and scarcity of feed materials, which have high competition between animals and humans. This will also aid in reducing environmental pollution in the society. In this regard, proper utilization of animal by-products such as rumen digesta can result in cheaper feed, reduction in competition and lower cost of production. Over the years, the utilization of animal by-products such as rumen digesta as feed in livestock feed has been successfully carried out without any adverse effect on the animals.