DOCSLIB.ORG

Annals of Basic and Applied Sciences December 2015, Volume 6, Number 1, (ISSN 2277-8756)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Annals of Basic and Applied Sciences December 2015, Volume 6, Number 1, (ISSN 2277-8756) 6 December 2015 Volume 6 SI.No 1 A Facile Spectrophotometric Determination of Osmium(VIII) in very low 1 - 6 concentrations using DL-Pencillamine as Chromogenic Reagent Bincy Joseph and Abraham Joseph 2 Evaluation of insecticidal and larvicidal activity and the detection of lipase 7 - 14 from Eupatorium odoratum leaf extracts. Soniya P.A., Navia Sebastian and Dr. Deepa G Muricken 3 Biosynthesis of Gold Nanoparticles using Bacteria and Fungi 15 - 21 Nivea Wilson, Nileena PV, Dhanya KC 4 Comparative Study on Antibacterial profiles of Kanakasava 22 - 25 Elizabeth P Thomas, Uma V Menon P, Afeeda Beegum M K, Athira MR, Darsana NC, and KS Dhaneesha Sivadas 5 Antibacterial effect of citrus fruit juice against enteric and non enteric 26 - 33 pathogenic bacteria Geenat Paul , Rigi George A , Rahima N A 6 Hydrogen Peroxide Scavenging Ability of Scoparia Dulci&Piper Longum Linn 34 - 40 ABAS Geetha T December 2015 7 Effect of plant growth promoting Proteus spp.on fenugreek 41 - 49 Vol. 6, No.1 Jimtha John C, Mrudula M M, Anisha C, and Radhakrishnan EK 8 A study on the antibiotic sensitivity test of selected isolates from facial 50 - 60 microflora and their susceptibility to commercial face washes Femina K K, Finciya A T, Hanna T V, Jasira V, Jini Joy P & Mabel Merlen Jacob 9 Bioactive peptides derived from milk: a review 61 - 65 Reshma Chandran P 5 1 0 2 Annals of Basic and Applied Sciences December 2015, Volume 6, Number 1, (ISSN 2277-8756). (Official publication of St Mary's College, Trichur - 680020, Kerala, India) Editor Dr Meera C R Department of Microbiology, St. Mary's College, Trichur - 680020 Kerala, India. Associate Editors Dr. Dhanya KC Dr. Mabel Merlen Jacob Department of Microbiology, Department of Microbiology, St. Mary's College, Trichur - 680020, St. Mary's College, Trichur - 680020, Kerala, India. Kerala, India. Editorial Board Dr. Regi Raphael K Dr. Kezia Kuruvila Dr. Sheeja T Tharakan Dr. Manju Madhavan Department of Botany, Department of Zoology, Department of Botany, Department of Botany, St. Mary's College, Vimala College, Vimala College, Vimala College, Trichur-680020, Trichur-680009, Trichur - 680009, Trichur - 680020 Kerala, India. Kerala, India. Kerala, India. Kerala, India. Dr Geetha T Dr. Manju Sebastian Dr. Rekha K Department of Chemistry, Department of Department of Botany, St. Mary's College, Chemistry, St. Mary's College, Trichur - 680020, St.Mary's College, Trichur - 20 Kerala, India. Trichur - 680020, Kerala, India. Kerala, India. Scientific Advisory Board Dr. Sr Jacintha C C Dr. K K Janardhanan Dr.C K K Nair Principal, Professor & Head, Director of Research, St. Mary's College, Department of Microbiology, St. Gregorios Dental Trichur - 680020, Amala Cancer Research Centre, College & Research Centre, Kerala, India. Trichur - 680555, Kerala, India. Kothamangalam – 686681, Kerala,India. Dr. S G PrapuIla Dr.Valsa A K Department of Fermentation, Department of Microbiology, Technology & Bioengineering, Sree Sankara College. Kalady, CFTRI. Mysore - 570020, Emakulam-683574, Karnataka, India. Kerala,India. Publishers St.Mary'sCollege,Trichur-680020.Kerala,India. A Facile Spectrophotometric Determination of Osmium(VIII) in very low concentrations using DL-Pencillamine as Chromogenic Reagent Bincy Joseph and Abraham Joseph* * Department of Chemistry ,University of Calicut, Calicut University Corresponding Author:Bincy Joseph,Dept.Of Chemistry,St.Mary’s College,Thrissur.Ph.No.9946355330 Email:[email protected]. ABSTRACT A simple spectrophotometric technique of high sensitivity and selectivity has been developed for the determination of trace amount of osmium(VIII) using DL- Pencillamine(DL- Pen) as a chromogenic reagent. The method is based on the formation of a pale pink complex at a fast pace at room temperature by the reaction of osmium(VIII) with DL-Pen in strongly acidic solution of pH 1 which absorb at 540 nm. The decrease of absorbance is directly proportional to osmium concentration and obeys the Beer’s law in the range of 0.15-1.50 µg/ml. The optimum reaction condition and 4 - other analytical requirements were evaluated. The molar absorptivity(5.05 x 10 LMol 1 -1 -3 cm ), Sandell's sensitivity(3.8 x 10 -2 -1 -1 µgcm ), detection limit(0.066 µgml ) and quantitation limit(0.20 µgml ) of the method were computed. Key words Osmium, Spectrophotometry, DL-Pencillamine , Chromogenic reagent. 1 Annals of Basic and Applied Sciences, Vol 6, No.1, Dec 2015 1.Introduction emission spectrometry, electron microprobe analysis, total reflection X- 6 2 Osmium(Z=76,5d 6s ) is a bluish ray fluorescence spectrometry, atomic white metal, the heaviest among the absorption spectrometry and neutron platinum metals, exhibits high specific activation analysis, the visible density(22.61g cm-3), high melting spectrophotometric methods seems to be 0 point(3050 C)and hardness(7.0Mhos the most appropriate analytical approach for the determination of toxic metals like scale) , occurs in ten different oxidation osmium, as it provides sensitive, precise states from Os(II) to Os(VIII). The and accurate measurements of suitable metal and its alloys are used for the analytes and offers practical and production of materials of extreme economical advantages over other hardness. methods. Most of these instruments are highly expensive and their day to day The volatile osmium tetroxide, maintenance cost is high and are also OsO is formed by the oxidation of 4, not free from various types of inherent osmium, is one of the most important interferences (Somasiri LLWet al., compounds of the element and is easily 1991,Honegger K .,et al 1987 Ramesh et al., identified by its characteristic unpleasant 1992).Besides,visible spectrophotometric odour. Osmium tetroxide is a powerful detection is much more viable as a toxic agent, harmful to eyes and useful technique to develop a portable respiratory tracts. So special precautions on-line or at line system. are needed in sample collection, storage and other procedures due to its strong Most of the existing well tendency of oxidation to volatile established spectrphotometric methods tetroxide and possible losses of the using the sulfur containing molecules element. The volatility of OsO4 makes and ligands like thiourea, thiocynate and the basis of separation of osmium from 1,5-diphenylcarbazide lack adequate its mixtures with other noble metals by sensitivity and selectivity and necessitate the separation of osmium by distillation. The reduction of OsO4 to extraction or distillation before the the black osmium dioxide, OsO2, by determination. biological substances made the basis of its use as a staining reagent for the Many organic reagents have been microscopic examination of tissues. proposed for spectrophotometric determination of osmium, including Compared to the various modern Perphenazine (Gowda et al., 1984) optical methods of analysis including inductively coupled plasma atomic In the flotation methods, the molar 2 Annals of Basic and Applied Sciences, Vol 6, No.1, Dec 2015 5 absorptivities are 2.2 x 10 at 655 nm ,2.0 containing ∼100 ml of water acidified 5 x 10 (with crystal violet and malachite with 3 ml of H2SO4 (1+1) wash, dry and green) (Balcerzak M. 1991) . Larger weigh the glass fragments of the amounts of osmium can be determined ampoule and calculate the weight of 2- 3 as osmate, OSO4 (ε = 2.75 x 10 at 340 OsO4 by difference. Dilute the osmium nm) (Ensafi et al 1991) .Other than direct solution with double distilled water till 1 spectrophotometric methods of ml contains precisely 1.5 mg of osmium. determination, certain reagents (Jamshid Perform all these operations in a fume et al 2000) are employed for the cupboard, and keep the osmium solution spectrophotometric determination of in a bottle with precision ground stopper osmium based on its catalytic activity. on account of the toxic properties and Derivative spectrophotometric methods offensive odour of the tetroxide( are also used for the determination of Marczenko Z 1986). osmium under different experimental conditions (Balcerzak M e t al., 1996, Pencillamine solution (1%): Balcerzak M et al.,1997, Kosiorek A et al., Dissolve 1g of reagent (HIMEDIA) in 2006) . 100 ml of double distilled water. Apparatus: The absorbance was In the present work, a reaction of measured and absorption spectra were Os(VIII) with DL-pencillamine have recorded using a systronics double beam been examined at various experimental uv-vis spectrophotometer 2201with conditions and the most suitable Quartz Cells (1cm). Digital pH meter analytical requirements have been (M.C. Dalal and Co. Madras) was used established for the quantitative for pH measurements. determination the element in pure Procedure:Aliquots of the stock solution aliquots and in the presence of various containing 0.15-1.5 µg of osmium(VIII) other metal ions. The proposed method were pipetted out into a series of 10 ml for the element offers many advantages standard flask and made up to the mark over the conventional using dil. H2SO4 (to maintain pH1). 3ml spectrophotometric methods. each of these solutions were taken and mixed with 0.1ml of 1% DL- 2.Experimental pencillamine solution and shaken well. A pink colour is developed in the Reagents and chemicals:Standard solution and measure the absorbance at osmium solution (1.5 mg/ml) : carefully 540 nm against the reagent blank. The broken an accurately weighed glass calibration graphs were constructed by ampoule containing ∼1 g of OsO4 (J.M. plotting the absorbance against the Chemicals Ltd., London)in a beaker amount of metal ions. 3 Annals of Basic and Applied Sciences, Vol 6, No.1, Dec 2015 3. Results and Discussion 0.75µg of osmium. It was noticed that an excess of the reagent does not have DL-pen reacts with osmium at any adverse effect on the sensitivity and room temperature to form a pink stability of the complex. coloured complex species having maximum absorbance at 540 nm. 3.3 Effect of time on absorbance Subsequent studies have been therefore carried out at this wavelength.
Load more

Recommended publications
  • Ecological Studies on Mango Leaf Webber (Orthaga Exvinaceahamp.)
    Internat. J. agric. Sci. Vol.2 No.2 July 2006 : (308-311) 308 Ecological studies on mango leaf webber (Orthaga exvinacea Hamp.) in Andhra Pradesh as a basis for IPM M. Kannan*1 and N. Venugopala Rao Department of Entomology, S.V. Agricultural College, TIRUPATI (A.P.) INDIA ABSTRACT The influence of ecological factors viz., biotic (Host plant) and abiotic factors (weather parameters) on the abundance and population fluctuation of leaf webber, Orthaga exvinacea (Hamp.) on mango under the conditions of Chittoor district were worked out. Peak incidence was observed during first fortnight of November (19.4 webs/tree). However, gradual increase was observed from the first fortnight of July (2.6 webs/tree) and declined during second fortnight of January (3.2 webs/tree). Correlation studies between incidence and weather parameters showed positive relationship with minimum temperature, relative humidity and rainfall and negative relationship with maximum temperature. None of the varieties was free from infestation. The infestation ranged from 7.80 to 29.47 webs/tree, 5.82 to 22.55 leaves/web and 1.92 to 29.47 larvae/tree. Variety Neelum showed less infestation, while Bangalore showed severe infestation and other varieties viz., Neeleghan, Cherakurasam, Mulgova, Rumani, Baneshan and Swarnajahangir have moderate infestation. Result of the study also revealed that older mango trees (15 and above years old) were more susceptible (18.26, 348.75, 121.61 webs/tree, webbed leaves/tree and larva/ tree, respectively) to leaf webber damage than young trees (0-5 years old). Key Words: Mango leaf webber, Ecological studies, Varietal susceptibility, Abiotic factors INTRODUCTION Impact of abiotic factors on population dynamics of leaf webber Mango, Mangifera indica is an important fruit crop of India.
    [Show full text]
  • Hemiptera: Miridae: Deraeocorinae) from India with Biological Note
    J. Entomol. Res. Soc., 20(3): 67-73, 2018 Research Article Print ISSN:1302-0250 Online ISSN:2651-3579 First Record of Termatophylum orientale Poppius (Hemiptera: Miridae: Deraeocorinae) from India with Biological Note Richa VARSHNEY1* Yeshwanth H. M.2 1 ICAR-National Bureau of Agricultural Insect Resources, P.B. No. 2491, H.A. Farm Post, Bellary Road, Hebbal, Bangalore-560024, INDIA. e-mail: *[email protected] 2 Department of Entomology, University of Agricultural Sciences, GKVK, Bangalore-560065 INDIA. e-mail: [email protected] ABSTRACT Termatophylum orientale Poppius is being reported for the first time from India. It was collected from Mangifera indica (Mango, Anacardiaceae), Carica papaya (Papaya, Caricaceae) and Peltophorum pterocarpum (Copperpod, Fabaceae) where it shares niche along with other predators like anthocorids, geocorids and pests like thrips, mites and lepidopteran larvae. For the first time rearing protocol and biology has been given for this mirid. Key words: Mango, Miridae, Deraeocorinae, Termatophylini, Termatophylum orientale, Thrips. Varshney, R., Yeshwanth, H.M. (2018). First record of Termatophylum orientale Poppius (Hemiptera: Miridae: Deraeocorinae) from India with biological note. Journal of the Entomological Research Society, 20(3), 67-73. 68 VARSHNEY, R., H. M., Y. INTRODUCTION Mirid bugs of the tribe Termatophylini are known to inhabit inflorescences, moth larval galleries or rolled bark. They are known to feed on thrips, besides feeding on nectar and pollen (Cassis, 1995; Cassis et al., 2011; Yasunaga et al., 2001). Three species of the genus Termatophylidea Reuter and Poppius were reported to attack on the cacao thrips, occupying the niche shared by anthocorids. It is assumed that these mirids are obligate predators and feed exclusively on thrips.
    [Show full text]
  • Heteroptera: Anthocoridae, Lasiochilidae)
    2018 ACTA ENTOMOLOGICA 58(1): 207–226 MUSEI NATIONALIS PRAGAE doi: 10.2478/aemnp-2018-0018 ISSN 1804-6487 (online) – 0374-1036 (print) www.aemnp.eu RESEARCH PAPER Annotated catalogue of the fl ower bugs from India (Heteroptera: Anthocoridae, Lasiochilidae) Chandish R. BALLAL1), Shahid Ali AKBAR2,*), Kazutaka YAMADA3), Aijaz Ahmad WACHKOO4) & Richa VARSHNEY1) 1) National Bureau of Agricultural Insect Resources, Bengaluru, India; e-mail: [email protected] 2) Central Institute of Temperate Horticulture, Srinagar, 190007 India; e-mail: [email protected] 3) Tokushima Prefectural Museum, Bunka-no-Mori Park, Mukoterayama, Hachiman-cho, Tokushima, 770–8070 Japan; e-mail: [email protected] 4) Department of Zoology, Government Degree College, Shopian, Jammu and Kashmir, 192303 India; e-mail: [email protected] *) Corresponding author Accepted: Abstract. The present paper provides a checklist of the fl ower bug families Anthocoridae th 6 June 2018 and Lasiochilidae (Hemiptera: Heteroptera) of India based on literature and newly collected Published online: specimens including eleven new records. The Indian fauna of fl ower bugs is represented by 73 5th July 2018 species belonging to 26 genera under eight tribes of two families. Generic transfers of Blap- tostethus pluto (Distant, 1910) comb. nov. (from Triphleps pluto Distant, 1910) and Dilasia indica (Muraleedharan, 1978) comb. nov. (from Lasiochilus indica Muraleedharan, 1978) are provided. A lectotype is designated for Blaptostethus pluto. Previous, as well as new, distribu-
    [Show full text]
  • Hyperlipaemic and Hyperglycaemic Effects of a Metabolic Peptide Hormone from the Neuronal Tissues of the Mango Leaf Webber Orthaga Exvinacea D
    D. Umadevi et al. IRJP 2012, 3 (2) INTERNATIONAL RESEARCH JOURNAL OF PHARMACY www.irjponline.com ISSN 2230 – 8407 Research Article HYPERLIPAEMIC AND HYPERGLYCAEMIC EFFECTS OF A METABOLIC PEPTIDE HORMONE FROM THE NEURONAL TISSUES OF THE MANGO LEAF WEBBER ORTHAGA EXVINACEA D. Umadevi*, M. Gokuldas and A.P. Ajaykumar Department of Zoology, Division of Insect Biochemistry and Physiology, University of Calicut, Kerala- 673635, India Article Received on: 06/01/12 Revised on: 09/02/12 Approved for publication: 18/02/12 *Email: [email protected] ABSTRACT The peptide hormone present in the brain-retrocerebral complexes of the mango leaf webber Orthaga exvinacea (Pyralidae: Lepidoptera) belonging to the adipokinetic hormone/red pigment-concentrating hormone family have different functions. The activity of the hormone extract tested both in vivo (heterologous bioassays against the polyphagous plant bug Iphita limbata) and in vitro clearly indicated that they are involved in lipid (adipokinetic hormones) and carbohydrate (hyperglycaemic hormones) release by activating fat body lipase and glycogen phosphorylase respectively. Injection of hormone extract (5 µl) containing one gland pair equivalent (gpe) elicited significant hyperlipaemic (up to 15%, P<0.001) and hyperglycaemic effects (up to 18%, P<0.05), whereas in the control, injection of 5 µl of insect saline did not evoke any such effects. The brain-retrocerebral complex extract showed significant effect on fat body lipid mobilization (up to 17%, P<0.05) and fat body sugar release (up to 18%, P<0.001). HPLC separation of the peptides followed by analysis of the fractions for activities confirmed that the peptide hormone extracted has a pivotal role in the mobilization of these metabolites.
    [Show full text]
  • I International Journal O International Journal Of
    International Journal Of Recent Scientific Research ISSN: 0976-3031 Volume: 7(3) March -2016 CHANGES IN THE TITRE OF ECDYSTEROIDS IN THE MANGO LEAF WEBBER, ORTHAGA EXVINACEA (HAMPSON) DURING DEVELOPMENT Remya, S and Gokuldas, M THE OFFICIAL PUBLICATION OF INTERNATIONAL JOURNAL OF RECENT SCIENTIFIC RESEARCH (IJRSR) http://www.recentscientific.com/ [email protected] Available Online at http://www.recentscientific.com International Journal of Recent Scientific International Journal of Recent Scientific Research Research Vol. 7, Issue, 3, pp. 9503-9508, March, 2016 ISSN: 0976-3031 RESEARCH ARTICLE CHANGES IN THE TITRE OF ECDYSTEROIDS IN THE MANGO LEAF WEBBER, ORTHAGA EXVINACEA (HAMPSON) DURING DEVELOPMENT Remya, S and *Gokuldas, M ARTICLE INFO Department ofABSTRACT Zoology, University of Calicut, Kerala, India 673 635 Article History: Investigations were carried out to estimate the qualitative and quantitative variation of ecdysteroids in the larval hemolymph and pupae of Orthaga exvinacea. The analyses were done by using HPLC Received December, 2015 st and FTIR. HPLC separations were carried out using Shimadzu system with a reverse phase column Received in revised form 21 (C18) of 250×4.6 mm i.d. The HPLC separations were carried out in binary gradient for 20 min. January, 2016 Acetonitrile 15% was used as solvent A, trifluoro acetic acid (TFA 0.1%) as solvent B. The eluents Accepted 06th February, 2016 th were monitored at 242 nm using a UV–visible detector. The main component found in the Published online 28 hemolymph and pupae was 20-ecdysteriod. The titre of ecdysteroid in the pupae showed a higher March, 2016 value (1.23 µg/pupae) than the ecdysteroid in the hemolymph of 6th (0.70 µg/10 µL equivalent) and7th (0.60 µg/10 µL equivalent) instar larvae.
    [Show full text]
  • EFFECTS of BLOOD FEEDING on the TRANSCRIPTOME of the MALPIGHIAN TUBULES in the ASIAN TIGER MOSQUITO AEDES ALBOPICTUS Thesis
    EFFECTS OF BLOOD FEEDING ON THE TRANSCRIPTOME OF THE MALPIGHIAN TUBULES IN THE ASIAN TIGER MOSQUITO AEDES ALBOPICTUS Thesis Presented in Partial Fulfillment of the Requirements for the Degree Master in Science in the Graduate School of The Ohio State University By Carlos J. Esquivel Palma, B.S. Graduate Program in Entomology The Ohio State University 2015 Master’s Examination Committee: Dr. Peter M. Piermarini, Advisor Dr. David L. Denlinger Dr. Andrew P. Michel Copyright by Carlos J. Esquivel Palma 2015 Abstract Mosquitoes are one of the major threats to human health worldwide. They are vectors of protozoans, arboviruses, and filarial nematodes that cause diseases in humans and animals. The Asian tiger mosquito Aedes albopictus is a vector of medically important arboviruses such as dengue fever, chikungunya fever, yellow fever, eastern equine encephalitis, La Crosse encephalitis, and West Nile fever. Control of these diseases often involves control of the mosquito vectors with chemical insecticides. However, the use of a limited number of chemicals with similar modes of actions has led to resistance in several mosquito species. The development of new insecticides with novel modes of action is considered a promising strategy to overcome resistance. The Piermarini lab has recently shown that the renal (Malpighian) tubules of mosquitoes are promising physiological targets to disrupt for killing mosquitoes via a novel mode of action. The Malpighian tubules play a critical role in the acute processing of blood meals, by mediating the rapid excretion of water and ions derived from the ingested blood. However, the physiological roles of Malpighian tubules during the chronic processing of blood meals after the initial diuresis is complete (~1-2 h after feeding) are not known.
    [Show full text]
  • Journal of the Entomological Research Society
    ISSN 1302-0250 Journal of the Entomological Research Society --------------------------------- Volume: 20 Part: 3 2018 JOURNAL OF THE ENTOMOLOGICAL RESEARCH SOCIETY Published by the Gazi Entomological Research Society Editor (in Chief) Abdullah Hasbenli Managing Editor Associate Editor Zekiye Suludere Selami Candan Review Editors Doğan Erhan Ersoy Damla Amutkan Mutlu Nurcan Özyurt Koçakoğlu Language Editor Nilay Aygüney Subscription information Published by GERS in single volumes three times (March, July, November) per year. The Journal is distributed to members only. Non-members are able to obtain the journal upon giving a donation to GERS. Papers in J. Entomol. Res. Soc. are indexed and abstracted in Biological Abstract, Zoological Record, Entomology Abstracts, CAB Abstracts, Field Crop Abstracts, Organic Research Database, Wheat, Barley and Triticale Abstracts, Review of Medical and Veterinary Entomology, Veterinary Bulletin, Review of Agricultural Entomology, Forestry Abstracts, Agroforestry Abstracts, EBSCO Databases, Scopus and in the Science Citation Index Expanded. Publication date: November 25, 2018 © 2018 by Gazi Entomological Research Society Printed by Hassoy Ofset Tel:+90 3123415994 www.hassoy.com.tr J. Entomol. Res. Soc., 20(3): 01-22, 2018 Research Article Print ISSN:1302-0250 Online ISSN:2651-3579 Palm Weevil Diversity in Indonesia: Description of Phenotypic Variability in Asiatic Palm Weevil, Rhynchophorus vulneratus (Coleoptera: Curculionidae) Sukirno SUKIRNO1, 2* Muhammad TUFAIL1,3 Khawaja Ghulam RASOOL1 Abdulrahman
    [Show full text]
  • Incidence and Distribution Pattern of Leaf Webber (Orthaga Exvinacea
    Journal of Entomology and Zoology Studies 2017; 5(2): 1196-1199 E-ISSN: 2320-7078 P-ISSN: 2349-6800 Incidence and distribution pattern of leaf webber JEZS 2017; 5(2): 1196-1199 © 2017 JEZS (Orthaga exvinacea Hamp.) on mango Received: 07-01-2017 Accepted: 08-02-2017 N Kasar N Kasar, JC Marak, UK Das and S Jha Department of Agricultural Entomology, Bidhan Chandra Abstract Krishi Viswavidyalaya, A field investigation was carried out to study the incidence and distribution pattern of mango leaf Mohanpur, Nadia, West Bengal, webber, Orthaga exvinacea Hampson during 2012-13 and 2013-14 at mango orchard of Bidhan Chandra 741252, India Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal. The results indicated that the most active period of mango leaf webber in both years was found from August to December. The distribution study of leaf JC Marak webs within the tree revealed that western and southern side had more number of webs in comparison to Department of Agricultural Entomology, Bidhan Chandra the northern and eastern side respectively. The differences were statistically significant. Correlation Krishi Viswavidyalaya, results indicated that maximum and minimum temperature had negative and significant effect (r = - Mohanpur, Nadia, West Bengal, 0.796** and -0.755**, respectively) on the leaf webber population. However, relationship of morning 741252, India relative humidity (r = +0.328**) was positively significant and evening relative humidity (r = -0.239) was negative and non-significant. Total rainfall (r = -0.370*) had negative and significant influence on UK Das leaf webber population. Department of Agricultural Entomology, Bidhan Chandra Keywords: Mango, O. exvinacea, seasonal incidence, distribution pattern, correlation Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, 1.
    [Show full text]
  • Efficacy of Clerodendrum Infortunatum and Chromolaena Odorata on Carbohydrate Content in Haemolymph of the Sixth Instar Larvae O
    [Nambiar et. al., Vol.5 (Iss.4): April 2018] ISSN: 2454-1907 DOI: https://doi.org/10.29121/ijetmr.v5.i4.2018.209 EFFICACY OF CLERODENDRUM INFORTUNATUM AND CHROMOLAENA ODORATA ON CARBOHYDRATE CONTENT IN HAEMOLYMPH OF THE SIXTH INSTAR LARVAE OF ORTHAGA EXVINACEA HAMPSON Jagadeesh G. Nambiar*1, K. R. Ranjini2 *1 Department of Zoology, Malabar Christian College, Calicut, India 2 Associate Professor, Department of Zoology, Malabar Christian College, Calicut, India Abstract: Orthaga exvinacea is one of the major pests of mango crop and the caterpillars defoliate the leaves and thereby reduce the crop yield. Use of synthetic insecticide is the quick method for the control this pest but its uncontrolled usage has resulted in serious lethal effects on non-target organisms and environmental pollution. Botanical insecticides are very effective, safe and ecologically acceptable. In the present study, the impact of methanolic leaf extracts of Clerodendrum infortunatum and Chromolaena odorata on carbohydrate concentration in the haemolymph of sixth instar larvae of O. exvinacea was studied under laboratory conditions. The different concentrations (1% to 5%) of each botanical treated mango leaves were fed to the sixth instar. After 48 hours, larvae were sacrificed to collect haemolymph and the quantitative estimation of carbohydrate has been done. The results showed that there was some noticeable decrease in the amount of carbohydrate in the treated larvae when compared to control. The decrease in level of carbohydrate concentration was correlated with the increase in concentration of botanicals. Among the botanicals tested C. odorata possessed more efficacy than that of C. infortunatum and this experiment reveals the potency of both botanicals to be used as natural biopesticides against this pest.
    [Show full text]
  • Bioefficacy of Leaf Extracts of Clerodendrum Infortunatum L. and Eupatorium Odoratum L
    J. G. Nambiar et al. Int. J. Res. Biosciences, 6(1), 19-25, (2017) International Journal of Research in Biosciences Vol. 6 Issue 1, pp. (19-25), January 2017 Available online at http://www.ijrbs.in ISSN 2319-2844 Research Paper Bioefficacy of leaf extracts of Clerodendrum infortunatum L. and Eupatorium odoratum L. on both quantitative and qualitative analysis of midgut protein of sixth instar larvae of Orthaga exvinacea Hampson (Lepidoptera: Pyralidae) Jagadeesh G. Nambiar, Ranjini K.R., Ranjini K.D., Najiya Beegum T.P. Department of Zoology, Malabar Christian College, Calicut-673001, Kerala, INDIA (Received November 15, 2016, Accepted December 08, 2016) Abstract The impact of methanolic leaf extract of Clerodendrum infortunatum and Eupatorium odoratum on protein concentration and protein profile in the midgut tissue of sixth instar larvae of Orthaga exvinacea were studied under laboratory conditions. The different concentrations (1%, 2%, 3%, 4%, and 5%) of each botanical treated mango leaves were fed to the sixth instar larvae. After 48 hours larvae were sacrificed to collect midgut tissue and analysis were done. The results of quantitative and qualitative estimation of protein content in the midgut tissue of both botanicals treated and control showed that there was some considerable decrease in the amount of protein and alterations in protein profile were observed in treated tissue compared to control. The decrease in level of protein concentration was correlated with the increase in concentration of botanicals. Formation of new protein bands and disappearance of some protein bands were noticed in protein profile of treated tissues with increase in botanical concentrations. Among the botanicals tested E.
    [Show full text]
  • Zootaxa, Taxonomic and Biological Notes on Cardiastethus Affinis And
    Zootaxa 1910: 59–68 (2008) ISSN 1175-5326 (print edition) www.mapress.com/zootaxa/ ZOOTAXA Copyright © 2008 · Magnolia Press ISSN 1175-5334 (online edition) Taxonomic and biological notes on Cardiastethus affinis and C. pseudococci pseudococci (Hemiptera: Heteroptera: Anthocoridae) in India KAZUTAKA YAMADA1, 3, K. BINDU2 & M. NASSER2 1Tokushima Prefectural Museum, Bunka-no-mori Park, Mukôterayama, Hachiman-chô, Tokushima, 770–8070 Japan. E-mail: [email protected] 2Department of Zoology, University of Calicut, Malappuram, Kerala, 673 635 India. E-mail: [email protected] (KB); [email protected] (MN) 3Corresponding author Abstract Cardiastethus affinis and C. pseudococci pseudococci were recognized in Kerala State, southern India: the latter is recorded from India for the first time. It is found that Cardiastethus affinis is associated with Orthaga exvinacea (Lepi- doptera: Pyralidae) and C. pseudococci pseudococci is associated with Opisina arenosella (Lepidoptera: Xylorictidae). Revised diagnoses and illustrations of both species are given. Biological notes for Indian species of Cardiastethus and a key to the three local species of the genus are provided. Key words: Heteroptera, Anthocoridae, Cardiastethus affinis, Cardiastethus pseudococci pseudococci, taxonomy, new record, biological control, hosts, India Introduction Cardiastethus Fieber, 1860 is a cosmopolitan genus in the family Anthocoridae, with approximately 45 described species (cf. Péricart 1972; Lattin & Stanton 1993). Eight species occur in Asia. Of these, C. affinis Poppius, 1909 and C. exiguus Poppius, 1913 have been recorded from India (Muraleedharan 1975; Mura- leedharan & Ananthakrishnan 1978; Nasser & Abdurahiman 1990). The species of Cardiastethus have attracted the attention of researchers who work in agro-ecosystems because they may include potential bio- control agents against major agricultural pests.
    [Show full text]
  • Studies on Biology of Mango Leaf Webber Orthaga Exvinacea
    International Journal of Chemical Studies 2020; 8(6): 2088-2091 P-ISSN: 2349–8528 E-ISSN: 2321–4902 www.chemijournal.com Studies on biology of mango leaf webber Orthaga IJCS 2020; 8(6): 2088-2091 © 2020 IJCS exvinacea (Hampson) Received: 07-08-2020 Accepted: 19-09-2020 Mallikarjun CJ, Suvarna Patil, Kotikal YK, Athani SI, Mastiholli AB, Mallikarjun CJ Naik KR, Vinaykumar MM and Ambika DS College of Horticulture, Bagalkot, Karnataka, India DOI: https://doi.org/10.22271/chemi.2020.v8.i6ad.11077 Suvarna Patil Assistant Professor, Department Abstract of Entomology, RHREC, The laboratory studies on biology of mango leaf webber was conducted at the Department of Dharwad, Karnataka, India Entomology, College of Horticulture, Bagalkot during 2018-19. The studies revealed that the mean preoviposition, oviposition and postoviposition periods lasted for 2.60±0.52, 4.10±0.74 and 1.90±0.74 Kotikal YK days, respectively. The larva moulted six times by passing through seven larval instars. The mean Professor, Department of st nd rd th th th th Entomology and Director of durations of 1 , 2 , 3 , 4 , 5 , 6 and 7 instar were 4.80±0.92, 4.65±0.90, 4.41±0.63, 4.16±0.87, Extension, UHS, Bagalkot, 4.52±0.51, 5.20±0.42 and 6.50±0.53 days, respectively with mean total larval period of 42.54±2.53 days. Karnataka, India The mean duration of male and female pupal stage were 11.80±0.63 days and 14.30±1.43 days respectively. The mean longevity of adult male and female were 4.10±0.74 days and 5.80±0.42 days, Athani SI respectively.
    [Show full text]