US010694750B2

(12 ) United States Patent ( 10 ) Patent No.: US 10,694,750 B2 Hirotomi et al. (45 ) Date of Patent: Jun . 30 , 2020

( 54 ) PLANT DISEASE CONTROL COMPOSITION WO WO 03/055303 A1 7/2003 AND PLANT DISEASE CONTROL METHOD WO WO 2009/037242 A2 3/2009 WO WO 2011/162397 A1 12/2011 WO WO 2013/110591 A1 8/2013 (71 ) Applicant: SUMITOMO CHEMICAL WO WO 2013/178661 A 12/2013 COMPANY, LIMITED , Tokyo ( JP ) WO WO 2014/083033 A1 6/2014 WO WO 2015/004260 A1 1/2015 (72 ) Inventors : Dai Hirotomi, Kasai ( JP ) ; Yasutaka Shimokawatoko, Takarazuka ( JP ) OTHER PUBLICATIONS ( 73 ) Assignee : SUMITOMO CHEMICAL Derwent abstract 2007-050901 ; abstracting JP 2006-347885 (Dec. COMPANY, LIMITED , Tokyo (JP ) 2006 ). * EPA Pesticide Fact Sheet Ipconazole , United States Environmen ( * ) Notice : Subject to any disclaimer , the term of this tal Protection Agency (2004 ) . * patent is extended or adjusted under 35 U.S. Office Action for U.S. Appl. No. 15 /746,551 , dated Jul . 3 , 2018. U.S.C. 154 (b ) by 0 days . International Preliminary Report on Patentability and English trans lation of the Written Opinion of the International Searching Author (21 ) Appl. No .: 15 /747,699 ity (PCT /IB /373 and PCT/ ISA / 237) for International Application No. PCT/ JP2016 /072072 , dated Jan. 30 , 2018 . ( 22 ) PCT Filed : Jul. 27 , 2016 International Preliminary Report on Patentability and English trans lation of the Written Opinion of the International Searching Author ( 86 ) PCT No .: PCT/ JP2016 /072074 ity (PCT / IB /373 and PCT/ ISA/ 237 ) for International Application $ 371 ( c ) ( 1 ) , No. PCT/ JP2016 /072075 , dated Jan. 30 , 2018 . (2 ) Date : Jan. 25 , 2018 International Search Report ( PCT/ ISA /210 ) for International Appli cation No. PCT/ JP2016 /072072 , dated Sep. 13 , 2016 . ( 87 ) PCT Pub . No .: WO2017 /018465 International Search Report (PCT / ISA / 210 ) for International Appli cation No. PCT/ JP2016 /072075 , dated Sep. 13 , 2016 . PCT Pub . Date: Feb. 2 , 2017 Peachey et al. , “Mesiterpro Crop Protection Handbook ," 2014 Centennial Edition , 2014 , pp . 9 , 191, 318-319 , 358-360 , 568-569 . (65 ) Prior Publication Data The Insecticide Resistance Action Committee , “Mode of Action Classification , ” Insecticide Resistance Management , Fourth Edi US 2018/0213796 A1 Aug. 2 , 2018 tion , Dec. 2014 , pp . 7 , 20 ( 4 pages total) . International Preliminary Report on Patentability and English trans ( 30 ) Foreign Application Priority Data lation of the Written Opinion of the International Searching Author ity (PCT / IB / 373 and PCT/ ISA / 237 ) for International Application Jul. 27 , 2015 ( JP ) 2015-147698 No. PCT/ JP2016 /072073 , dated Jan. 30 , 2018 . Dec. 22 , 2015 ( JP ) 2015-250254 International Preliminary Report on Patentability and English trans lation of the Written Opinion of the International Searching Author (51 ) Int. Cl. ity (PCT / IB / 373 and PCT/ ISA / 237 ) for International Application No. PCT/ JP2016 / 072074 , dated Jan. 30 , 2018 . AOIN 43/653 ( 2006.01 ) International Preliminary Report on Patentability and English trans AOIN 4738 (2006.01 ) lation of the Written Opinion of the International Searching Author AOIN 63/22 (2020.01 ) ity (PCT / IB /373 and PCT/ ISA / 237) for International Application (52 ) U.S. Cl. No. PCT/ JP2016 /072076 , dated Jan. 30 , 2018 . CPC A01N 63/22 ( 2020.01) ; A01N 43/653 International Search Report (PCT / ISA /210 ) for International Appli ( 2013.01) cation No. PCT/ JP2016 /072073 , dated Sep. 13 , 2016 . (58 ) Field of Classification Search International Search Report (PCT /ISA /210 ) for International Appli CPC combination set( s ) only . cation No. PCT/ JP2016 /072074 , dated Sep. 13 , 2016 . See application file for complete search history . (Continued ) (56 ) References Cited Primary Examiner John Pak U.S. PATENT DOCUMENTS ( 74 ) Attorney , Agent, or Firm - Birch , Stewart , Kolasch & Birch , LLP 6,995,007 B2 2/2006 Gunner et al. 7,666,406 B2 2/2010 Gunner et al. 2011/0110906 A1 5/2011 Andersch et al . 2015/0282483 A1 10/2015 Sawada et al. (57 ) ABSTRACT 2017/0188584 A1 * 7/2017 Jabs A01N 63/02 The present invention addresses the problem of providing a FOREIGN PATENT DOCUMENTS composition that has excellent plant disease controlling effects . Provided is a plant disease control composition that JP 10-109913 A 4/1998 JP 2006-347885 A 12/2006 includes a new strain of Bacillus, APM - 1, which has been JP 2011-519824 A 7/2011 deposited under ATCC Accession number PTA - 4838 , and JP 2014-139223 A 7/2014 one or more ergosterol biosynthesis inhibitor. JP 2015-504905 A 2/2015 JP 2015-519352 A 7/2015 JP 2016-155862 A 9/2016 7 Claims, No Drawings US 10,694,750 B2 Page 2

( 56 ) References Cited Kim et al ., " Ethaboxam : a new Oomycetes fungicide, ” BCPC Conference Pests & Diseases , vol . 1 , 2002, pp . 377-382 (1 page , abstract only provided ). OTHER PUBLICATIONS U.S. Office Action for U.S. Appl. No. 15 / 746,551, dated Jan. 8 , International Search Report (PCT / ISA/ 210 ) for International Appli 2019 . cation No. PCT/ JP2016 /072076 , dated Sep. 13 , 2016 . U.S. Office Action for U.S. Appl . No. 15/ 747,513, dated Oct. 1 , MacBean ( Ed . ), “ The Pesticide Manual, ” A World Compendium , 2018 . Sixteenth Edition , BCPC , 2012, pp . 97 , 117-118 , 122-123, 528-529 , Bartlett et al. , “ The Strobilurin Fungicides, ” Pest Management 559-560 , 577 , 671-672 , 863-865 , 868-869 , 1021-1022 , 1216 (27 Science , vol . 58 , 2002, pp . 649-662 . pages total) . U.S. Office Action for U.S. Appl. No. 15 /747,628 , dated Jul. 11, Hilber et al. , “ Comparative resistance patterns of fludioxonil and 2019 . vinclozolin in Botryotinia fuckeliana , ” Journal of Phytopathology , vol . 143, No. 7 , 1995 , pp . 423-438 ( 1 page , only abstract provided ) . * cited by examiner US 10,694,750 B2 1 2 PLANT DISEASE CONTROL COMPOSITION ofmetconazole , tebuconazole, prothioconazole, ipconazole , AND PLANT DISEASE CONTROL METHOD difenoconazole , triticonazole , triadimenol, and bromcon azole . TECHNICAL FIELD [4 ] The composition according to any one of [1 ] to [3 ] 5 comprising the ergosterol biosynthesis inhibitor in an The present invention relates to composition for con amount of 10-10 to 1.5x107 g per 1010 cfu of Bacillus strain trolling plant diseases and a method for controlling plant APM - 1 . diseases . [ 5 ] A plant seed or a vegetative propagation organ compris ing Bacillus strain APM - 1 (New strain of Bacillus , APM - 1 ) BACKGROUND ART 10 deposited under ATCC Accession No. PTA -4838 and one or more ergosterol biosynthesis inhibitor. New strain of Bacillus, APM - 1 (deposited under ATCC [ 6 ] The plant seed or a vegetative propagation organ accord Accession No. PTA - 4838 ) , has been known as an active ing to [ 5 ] wherein the ergosterol biosynthesis inhibitor is ingredient of compositions for controlling plant diseases and selected from the group consisting of tetraconazole , met disclosed , for example , in Patent Document 1. Also , ergos 15 conazole , tebuconazole , prothioconazole , ipconazole , terol biosynthesis inhibitors were known as an active ingre difenoconazole , triticonazole , propiconazole , triadimenol, dient of compositions for controlling plant diseases and prochloraz , fluquinconazole , hexaconazole , epoxiconazole , disclosed , for example , in Non - Patent Document 1. There is flutriafol , triadimefon , pefurazoate and bromconazole . need for a material which is still more effective for control- 20 to[ 7 ][ The 5] or plant [6 ] seedcomprising or vegetative 104 to propagation 1014 cfu of organ Bacillus according strain ling plant diseases. APM - 1 and 0.000001 to 15 g of the ergosterol biosynthesis inhibitor, per 1 kg of the seed or vegetative propagation PRIOR ART DOCUMENTS organ . [8 ] A method for controlling plant diseases, comprising a Patent Documents 25 step of applying Bacillus strain APM - 1 (New strain of Bacillus , APM - 1 ) deposited under ATCC Accession No. Patent Document 1 : WO 2003/055303 PTA -4838 and one or more ergosterol biosynthesis inhibitor to a plant or a plant cultivation site . Non - Patent Document [9 ] The method for controlling plant diseases according to 30 [8 ] wherein the plant is a genetically modified plant. Non -Patent Document 1 : The Pesticide Manual- 16th edition (BCPC , ISBN : 978-1-901396-86-7 ) Effect of Invention DISCLOSURE OF THE INVENTION The present invention provides an excellent composition 35 for protecting seeds or vegetative propagation organs and Problems to be Solved by the Invention plants grown therefrom from plant diseases . Damage from plant disease is a cause of considerable loss DESCRIPTION OF EMBODIMENTS of crop production , and there is a need to control such plant disease more effectively . Thus , it is an object of the present 40 The composition for controlling plant diseases of the invention to provide a composition having an excellent present invention (hereinafter referred to as “ the present controlling effect against plant diseases . composition ” ) contains Bacillus strain APM -1 (New strain of Bacillus, APM - 1 ) deposited under ATCC Accession No. PTA -4838 (hereinafter referred to as “ the present bacterial Means for Solving the Problems 45 strain ” ) and one or more ergosterol biosynthesis inhibitor The present inventors intensively studied to achieve the (hereinafter referred to as “ the present compound ” ) . above object and have found that a composition comprising The present bacterial strain has been disclosed in WO Bacillus strain APM - 1 which has been deposited under 2003/055303 and deposited under the name “ New strain of ATCC Accession No. PTA -4838 (New strain of Bacillus, 50 ATCCBacillus (, American APM - 1 ” underType ATCC Culture Accession Collection No. ). PTA WO -4838 2003 at/ APM - 1 ) and one or more ergosterol biosynthesis inhibitor 055303 describes that the strain is most similar to Bacillus has an excellent controlling effect against plant diseases . amyloliquefaciens. The present bacterial strain is available Thus, the present invention includes the following [ 1 ] to from ATCC and can be cultured by a known procedure . The [ 9 ] . culture may be used as it is or may be separated and [ 1 ] A composition for control plant diseases comprising 55 concentrated using a conventional industrial technique , such Bacillus strain APM - 1 (New strain of Bacillus, APM - 1 ) as, not limited to , membrane separation , centrifugal separa deposited under ATCC Accession No. PTA -4838 and one or tion , or filtration separation . The fraction of the present more ergosterol biosynthesis inhibitor. bacterial strain thus obtained may be used directly as it [ 2 ] The composition according to [ 1 ] wherein the ergosterol contains certain water in the present composition , or if biosynthesis inhibitor is selected from the group consisting 60 necessary , a dried product obtained by a dry method , such as of tetraconazole , metconazole , tebuconazole , prothiocon freeze- dry or spray drying , may be used as the present azole , ipconazole , difenoconazole , triticonazole , propicon bacterial strain . azole , triadimenol, prochloraz , fluquinconazole, hexacon In the present composition for controlling plant diseases , azole , epoxiconazole , flutriafol, triadimefon , pefurazoate the present compound to be used in combination with the and bromconazole . 65 presentbacterial strain is not limited , so long as it has an has [ 3 ] The composition according to [ 1 ] wherein the ergosterol inhibitory effect on ergosterol biosynthesis , but includes , for biosynthesis inhibitor is selected from the group consisting example , tetraconazole , metconazole, tebuconazole , proth US 10,694,750 B2 3 4 ioconazole , ipconazole , difenoconazole , triticonazole , Epoxiconazole is a known compound and has been propiconazole , triadimenol, prochloraz, fluquinconazole , described , e.g., on page 420 in " The Pesticide Manual- 16th hexaconazole , epoxiconazole , flutriafol, triadimefon , edition (Published by BCPC ): ISBN 978-1-901396-86-7 ” . pefurazoate and bromconazole , and preferably , metcon Epoxiconazole can be obtained from a commercially avail azole , tebuconazole , prothioconazole , ipconazole , difeno- 5 able formulation or produced by a known method . conazole , triticonazole , triadimenol, and bromconazole. Flutriafol is a known compound and has been described , Tetraconazole is a known compound and has been e.g., on page 556 in “ The Pesticide Manual - 16th edition described , e.g., on page 1087 in “ The Pesticide Manual- 16th ( Published by BCPC ) : ISBN 978-1-901396-86-7 ” . Flutria edition (Published by BCPC ): ISBN 978-1-901396-86-7 ” . fol can be obtained from a commercially available formu Tetraconazole can be obtained from a commercially avail 10 lation or produced by a known method . able formulation or produced by a known method . Triadimefon is a known compound and has been Metconazole is a known compound and has been described , e.g., on page 1135 in “ The Pesticide Manual- 16th described , e.g., on page 744 in “ The Pesticide Manual - 16th edition (Published by BCPC ) : ISBN 978-1-901396-86-7 ” . edition (Published by BCPC ). ISBN 978-1-901396-86-7 ” . 15 Triadimefon can be obtained from a commercially available Metconazole can be obtained from a commercially available formulation or produced by a known method . formulation or produced by a known method . Pefurazoate is a known compound and has been Tebuconazole is a known compound and has been described , e.g., on page 858 in “ The Pesticide Manual- 16th described , e.g. , on page 1063 in “ The Pesticide Manual - 16th edition (Published by BCPC ) : ISBN 978-1-901396-86-7 ” . edition (Published by BCPC ): ISBN 978-1-901396-86-7 ” . 20 Pefurazoate can be obtained from a commercially available Tebuconazole can be obtained from a commercially avail formulation or produced by a known method . able formulation or produced by a known method . Bromconazole is a known compound and has been Prothioconazole is a known compound and has been described , e.g. , on page 135 in “ The Pesticide Manual- 16th described , e.g., on page 956 in “ The Pesticide Manual- 16th edition (Published by BCPC ) : ISBN 978-1-901396-86-7 ” . edition (Published by BCPC ) : ISBN 978-1-901396-86-7 " . 25 Bromconazole can be obtained from a commercially avail Prothioconazole can be obtained from a commercially avail able formulation or produced by a known method . able formulation or produced by a known method . The present composition can be prepared typically by Ipconazole is a known compound and has been described , mixing the present bacterial strain and the present com e.g., on page 658 in “ The Pesticide Manual - 16th edition pound , respectively , with a solid carrier or a liquid carrier , ( Published by BCPC ) : ISBN 978-1-901396-86-7 ” . Ipcon- 30 with addition of a surfactant or other auxiliary agents for azole can be obtained from a commercially available for formulation if necessary, followed by combining the present mulation or produced by a known method . bacterial strain formulation and the compound formulation Difenoconazole is a known compound and has been thus obtained . Alternatively , the present composition can be described , e.g. , on page 348 in “ The Pesticide Manual - 16th prepared by mixing the present bacterial strain with the edition (Published by BCPC ): ISBN 978-1-901396-86-7 ” . 35 present compound in advance , adding a solid carrier or a Difenoconazole can be obtained from a commercially avail liquid carrier , with addition of a surfactant or other auxiliary able formulation or produced by a known method . agents for formulation if necessary , followed by formulating Triticonazole is a known compound and has been into a single formulation . described , e.g., on page 1171 in “ The Pesticide Manual- 16th Examples of the solid carrier include mineral fine pow edition (Published by BCPC ). ISBN 978-1-901396-86-7” . 40 ders , such as kaolin clay, pyrophyllite clay, bentonite ,mont Triticonazole can be obtained from a commercially available morillonite , diatomaceous earth , synthetic hydrous silicon formulation or produced by a known method . oxide , acidic clay, talc , clay, ceramic , quartz , sericite , ver Propiconazole is a known compound and has been miculite , pearlite , Oya stone, anthracite , limestone , coalite , described , e.g. , on page 942 in “ The Pesticide Manual- 16th and zeolite , inorganic compounds, such as sodium chloride, edition (Published by BCPC ) : ISBN 978-1-901396-86-7 " . 45 carbonate , sulfate , nitrate , and urea , organic fine powders , Propiconazole can be obtained from a commercially avail such as rice hulls , bran , wheat flour, and peat moss. able formulation or produced by a known method . Examples of the liquid carrier include water, vegetable oil , Triadimenol is a known compound and has been animal oil, and mineral oil. Examples of the auxiliary described , e.g. , on page 1136 in “ The Pesticide Manual - 16th substance for formulation include anti- freezing agents , such edition (Published by BCPC ) : ISBN 978-1-901396-86-7” . 50 as ethylene glycol, and propylene glycol, and thickening Triadimenol can be obtained from a commercially available agents , such as carboxymethyl cellulose , and xanthan gum . formulation or produced by a known method . The present composition may contain the present bacterial Prochloraz is a known compound and has been described , strain in an effective amount, for example , at least 104 cfu / g , e.g., on page 918 in “ The Pesticide Manual- 16th edition typically 104 to 1013 cfu /g , and preferably 10 % to 1012 cfu /g (Published by BCPC ). ISBN 978-1-901396-86-7 ” . Prochlo- 55 of the present composition . raz can be obtained from a commercially available formu The present composition may contain the present com lation or produced by a known method . pound in an effective amount, for example , typically 0.0001 Fluquinconazole is a known compound and has been to 0.90 g , preferably 0.001 to 0.80 g , per 1 g of the present described , e.g., on page 538 in “ The Pesticide Manual - 16th composition . edition (Published by BCPC ) : ISBN 978-1-901396-86-7 ” . 60 The present composition typically contains 10-10 to 1.5x Fluquinconazole can be obtained from a commercially avail 107 g , preferably 10-7 to 10 % g ,more preferably 10-5 to 102 able formulation or produced by a known method . g of the present compound per 1010 cfu of the present Hexaconazole is a known compound and has been bacterial strain . described in , e.g., on page 607 in “ The Pesticide Manual The term " effective amount” as used herein refers to an 16th edition (Published by BCPC ) : ISBN 978-1-901396-86- 65 amount of the present bacterial strain and the present com 7” . Hexaconazole can be obtained by a known method for pound that is able to exert the controlling effect against plant the production . diseases . US 10,694,750 B2 5 6 The method of the invention for controlling plant diseases the kind of plant disease to be targeted , and the occurrence (hereinafter referred to as “ the present controlling method ” ) frequency , the formulation form , the treatment period , the comprises a step of applying the present bacterial strain and treatment method , the place to be treated , the weather one or more of the present compounds to a plant or a plant condition or the like , and when a stem and a leaf of the plant cultivation site . 5 or a soil where the plant grows is treated , the amount of the In the present controlling method , the present bacterial present bacterial strain for the treatment is usually 10 % to strain and the present compound to be used are typically 1019 cfu , preferably 10 to 1017 cfu , per 1 ha, and the amount those which have been formulated and may be applied as of the present compound for the treatment is usually 10 to separate formulations or as a present composition . The 5000 g, preferably 20 to 2000 g , per 1 ha . The composition separate formulations may be applied simultaneously or 10 in a form of wettable powder , water dispersible granules or independently . the like may be used by diluting with water so that the In the present controlling method , the present bacterial concentration of the present bacterial strain is usually 10 % to strain and the present compound are applied in an effective 1012 cfu / L and that the concentration of the present com amount. pound is usually 0.0005 to 1 % by weight . The composition In the present invention , examples of the cultivation site 15 in a form of dustable powder or granules may be used as it of the plant include paddy field , cultivated field , tea field , is . fruit orchard , non -agricultural land , seedling tray and nurs In the seed treatment or vegetative propagation organ ery box , nursery soil and nursery mat, water culture medium treatment, the amount of the present bacterial strain is in hydroponic farm , and the like. The plant diseasemay have usually 104 to 1014 cfu , preferably 10 % to 1013 cfu per 1 kg already or not yet occurred in a cultivation site of plant or a 20 of the seed or vegetative propagation organ , and the amount place of disease occurrence . of the present compound is usually 0.000001 to 15 g , In the present controlling method , examples of the preferably 0.0001 to 10 g, per 1 kg of the of seed or method for treating the present bacterial strain and the vegetative propagation organ . present compound include foliage treatment, soil treatment, The weight of the seed or vegetative propagation organ root treatment, seed treatment and vegetative propagation 25 means the weight thereof when treating with the present organ treatment. bacterial strain and the present compound or other agricul Examples of the foliage treatment include treatment of the tural chemicals before seeding or burying of the same. surface of the cultivated plant with spraying onto the foliage By treating the seed or vegetative propagation organ as and stem . described above , a seed or vegetative propagation organ Examples of the root treatment include immersing whole 30 comprising the present bacterial strain and one or more plant or a root of the plant in a solution containing the compounds of the invention can be obtained . An adjuvant present bacterial strain and the present compound , as well as may be admixed if necessary during the seed treatment or attaching a solid preparation containing the present bacterial vegetative propagation organs treatment. strain , the present compound and a solid carrier to a root of Examples of the plant to which the present invention is the plant. 35 applicable include the followings . Examples of the soil treatment include soil broadcast, soil Agricultural crops: cereal crops, such as corn , wheat , incorporation and chemical irrigation to soil . barley, rye , oat , sorghum ; pseudocereals , such as buckwheat; Examples of the seed treatment and vegetative propaga pulses, such as soybean , peanut; cotton ; sugar beet; rice ; tion organ treatment include applying seed treatment or oilseed rape ; sunflower; sugar cane; tobacco ; hop . vegetative propagation treatment using the present compo- 40 Vegetables : solanaceous crops (eggplant , tomato , potato , sition, specifically , such as spray treatment wherein a sus chili pepper, green pepper, etc. ), cucurbitaceae crops ( cu pension of the present composition is sprayed onto the cumber, pumpkin , zucchini, watermelon , melon , orienta surface of the seed or the vegetative propagation organ , wet melon , etc. ) , cruciferous vegetables ( radish , turnip , horse powder coating treatment wherein the present composition radish , kohlrabi, chinese cabbage, cabbage , mustard , broc in a form of wettable powder is coated onto moist seed or 45 coli, cauliflower , etc. ), asteraceae vegetables (burdock , gar vegetative propagation organ , smearing treatment wherein a land chrysanthemum , artichoke , lettuce , etc. ) , liliaceae liquid of the present composition prepared from wettable vegetables (green onion , onion , garlic , asparagus, etc. ), powder , emulsifiable concentrate or flowable formulation of umbelliferae vegetables (carrot , parsley, celery , parsnip , the present composition , with addition of water if necessary , etc.) , chenopodiaceae vegetables (spinach , chard , etc.) , is applied onto seed or vegetative propagation organ , immer- 50 labiatae vegetables (perilla , mint, basil, etc. ) , leguminous sion treatment wherein seeds or vegetative propagation crops (pea , kidney bean , adzuki bean, broad bean , chickpea , organs are immersed in a liquid containing the present etc.) , strawberry , sweet potato, yam , taro , konjac, ginger , composition for a certain period of time, and film coating okra . treatment and pellet coating treatment of seeds with the Fruit trees: pome fruits (apple , Japanese pear, common present composition . 55 pear, Chinese quince, quince, etc. ), stone fruits (peach , In the present invention , the simply described “ plant”29 plum , nectarine , Japanese plum , cherry , apricot, prune, etc.) , encompasses in its meaning “ a seed of the plant” and “ a citrus fruits ( Satsuma mandarin , orange, lemon , lime, grape vegetative propagation organ of the plant" . fruit, etc.) , nuts ( chestnut, walnut, hazel nut, almond , pista The term “ vegetative propagation organ ” as used herein chio , cashew nut , macadamia nut, etc.) , berries ( blueberry, means a part of root , stem , leaf or the like of the plant having 60 cranberry , blackberry , raspberry , etc.) , grape, Japanese per the ability to grow when it is separated from the body and simmon , olive , loquat , banana, coffee , date palm , coconut placed on soil , such as flower bulb , potato tuberous root, palm , oil palm . stem tuber , scaly bulb , corm , rhizophore , and strawberry Trees other than fruit trees : tea, mulberry , flowering trees runner. (azalea , camellia , hydrangea , sasanqua , Japanese star anise , In the present controlling method , the amount of the 65 cherry, tulip tree , crape myrtle , orange osmanthus , etc.) , present bacterial strain and the present compound in the street trees ( ash tree , birch , dogwood , eucalyptus, ginkgo , treatment varies depending on the kind of plant to be treated , lilac, maple tree , oak , poplar , cercis , Chinese sweet gum , US 10,694,750 B2 7 8 plane tree , zelkova, Japanese arborvitae, fir tree, Japanese metabolizing enzyme ( glyphosate oxidase gene , goxv247 ) hemlock , needle juniper, pine, spruce, yew , elm , horse from Ochrobactrum (Ochrobactrum anthropi strain LBAA ) , chestnut, etc.) , coral tree , podocarpus , cedar, Japanese or EPSPS gene having glyphosate -tolerant mutation cypress , croton , Japanese spindle tree, Japanese photinia . (mepsps , 2mepsps ) from corn . There are glyphosate - tolerant Grasses : zoysia (zoysiagrass , Zoysia matrella , etc. ), ber- 5 genetically modified varieties with respect to plants such as muda grasses (Cynodon dactylon , etc.) , bent grasses ( Agros corn (Zea mays L.) , soybean (Glycine max L.) , cotton tis alba , creeping bent grass , hiland bent, etc.) , blueglasses (Gossypium hirsutum L.) , sugar beet ( Beta vulgaris ) , canola (meadow grass , bird grass , etc. ), fescue (tall fescue, chew ( Brassica napes, Brassica rapa ) , alfalfa (Medicago sativa ), ings fescue, creeping red fescue, etc. ), ryegrasses (darnel , potato (Solanum tuberrosum L ), wheat ( Triticum aestivum ), rye grass , etc.) , orchard grass, timothy grass. 10 and creeping bent grass (Agrostis stolonifera ). Others : flowers ( rose, carnation , chrysanthemum , prairie Some glyphosate - tolerant genetically modified plants are gentian , gypsophila , gerbera , marigold , Salvia, Petunia, commercially available . For example , a genetically modified verbena , tulip , aster , gentian , lily , pansy , cyclamen , orchid , plant expressing glyphosate - tolerant EPSPS from Agrobac convallaria , lavender, stock , ornamental cabbage , primula , terium has been marketed under the trade name such as poinsettia , gladiolus , cattleya , daisy , cymbidium , begonia , 15 Roundup Ready® , a genetically modified plant expressing etc. ), bio - fuel plants ( Jatropha, safflower , camelina, switch glyphosate metabolizing enzyme from Bacillus with grass , Miscanthus, reed canary grass, giant reed , kenaf, enhanced metabolic activity by gene shuffling has been cassava , willow , etc. ), ornamental plants . marketed under the trade name such as Optimum® GAT® , The present invention is preferably applied to cereal crops Optimum® Gly canola , and a genetically modified plant or millets . The present invention is more preferably applied 20 expressing EPSPS gene having glyphosate - tolerant mutation to corn , wheat, sorghum , and soybean . has been marketed under the trade name GlyTol® . In the present invention , the variety of plant is not limited Examples of plants conferred with herbicide - tolerance by so long as it is commonly cultivated . The plants of such gene recombination technology include glufosinate - tolerant varieties include plants which have been conferred with one genetically modified plants which have been introduced with or more useful trait by a classical breeding technique or a 25 phosphinothricin N - acetyltransferase (PAT ) gene (bar ) of the genetic engineering technique ( genetically modified plant) glufosinate metabolizing enzyme from Streptomyces ( Strep as well as stack varieties obtained by crossing such geneti tomyces hygroscopicus) , phosphinothricin N - acetyltrans cally modified plants . ferase gene (pat ) of the glufosinate metabolizing enzyme Such useful characters include tolerance to herbicide, pest from Streptomyces ( Streptomyces viridochromogenes ), a resistance, disease resistance , stress tolerance , and improved 30 synthesized pat gene, or the like. There are glufosinate quality of crops such as modified fatty acid residue compo tolerant genetically modified varieties with respect to plants sition of oils and fats. such as corn, soybean , cotton , canola , rice (Oryza sativa L.) , Examples of the genetically modified plant include those sugar beet , and cichory (Cichori intybus ). listed in the genetically modified crop registration database Some glufosinate -tolerant genetically modified plants are (GM APPROVAL DATABASE ) in the electronic informa- 35 commercially available . A genetically modified plant tion site ( http://www.isaaa.org/) of the INTERNATIONAL expressing glufosinate metabolizing enzyme (bar , pat ) from SERVICE for the ACQUISITION of AGRI- BIOTECH Streptomyces has been marked under a trade name including APPLICATIONS ( ISAAA ). More specifically, the plant Liberty Link® . may be a plant which has been conferred with an environ Examples of herbicide - tolerant genetically modified mental stress tolerance, a disease resistance , a herbicide 40 plants include genetically modified plants which have been tolerance, a pest resistance or the like , or a plant wherein its introduced with the gene (bxn ) of nitrilase , which is a trait has been modified with respect to growth and yield , bromoxynil- metabolizing enzyme from Klebsiella (Kleb quality of product, sterility or the like, by genetic recombi siella pneumoniae subsp . Ozaenae) . Bromoxynil -tolerant nation technology genetically modified varieties have been produced for plants Examples of the plant conferred with a herbicide toler- 45 such as canola , cotton , tobacco (Nicotiana tabacum L.) and ance by gene recombination technology include genetically have been marked under a trade name including Navigator® modified plants conferred with a tolerance to protoporphy canola , or BXN? . rinogen oxidase (herein after referred to as PPO ) herbicides Examples of herbicide -tolerant genetically modified such as flumioxazin ; 4 -hydroxyphenyl pyruvic acid dioxy plants include genetically modified carnation (Dianthus genase (hereinafter abbreviated as HPPD ) inhibitors such as 50 caryophyllus) which has been introduced with ALS herbi isoxaflutole , mesotrione ; acetolactate synthase (hereinafter cide - tolerant ALS gene ( SurB , S4 -HrA ) from tobacco as a referred to as ALS ) inhibitors such as imazethapyr, thifen selectable marker . Also , a genetically modified larvae ( Li sulfuron methyl; 5 -enolpyruvylshikimate -3 -phosphate syn num usitatissumum L.) which has been introduced with ALS thase (hereinafter referred to as EPSP ) inhibitors such as herbicide- tolerant ALS gene from Arabidopsis ( Arabidopsis glyphosate; glutamine synthetase inhibitors such as glufo- 55 thaliana ) has been developed under the trade name CDC sinate ; auxin herbicides such as 2,4 - D , dicamba ; and herbi Triffid Flax . Also , a genetically modified soybean which has cides such as bromoxynil. been introduced with ALS herbicide -tolerant ALS gene Examples of the plant conferred with a herbicide toler (csr1-2 ) from Arabidopsis has been developed under the ance by gene recombination technology include glyhosate trade name Cultivance® . Furthermore , there are sulfonylu tolerant genetically modified plants which have been intro- 60 rea /imidazolinone herbicide -tolerant genetically modified duced with one or more gene selected from glyphosate corn which has been introduced with ALS herbicide- tolerant tolerant EPSPS gene (CP4 epsps ) from Agrobacterium ALS gene ( zm -hra ) from corn , and sulfonylurea herbicide tumefaciens strain CP4 ; glyphosate metabolizing enzyme tolerant genetically modified soybean which has been intro gene (gat4601 , gat6421) which is a gene of glyphosate duced with ALS herbicide - tolerant ALS gene (gm -hra ) from metabolizing enzyme (glyphosate N -acetyl transferase ) 65 soybean . from Bacillus (Bacillus licheniformis ) modified by gene Examples of plants conferred with herbicide - tolerance by shuffling to enhance the metabolic activity ; glyphosate gene recombination technology include isoxaflutole - tolerant US 10,694,750 B2 9 10 genetically modified soybean which has been introduced soil bacterium Bt bacteria . Examples of the delta -endotoxin with HPPD herbicide- tolerant HPPD gene (hppdPFW 336 ) that confers a pest resistance to coccinella insects include from Pseudomonas (Pseudomonas fluorescens strain A32 ) Cry3A , mCry3A (modified Cry3A ) , Cry3Bb1, Cry34Ab1, and mesotrione- tolerant genetically modified soybean which and Cry35Ab1. has been introduced with HPPD gene (avhppd -03 ) from oats 5 Examples of the plant conferred with a pest resistance to (Avena sativa ). multipter insects by genetic recombination technology Examples of plants conferred with herbicide - tolerance by include genetically modified corn , which has been intro gene recombination technology include 2,4 - D - tolerant duced with a synthetic gene encoding a hybrid protein genetically modified corns, genetically modified soybeans , eCry3.1Ab , which is a hybrid protein of Cry3A and Cry1Ab genetically modified cottons which have been introduced 10 derived from soil bacteria Bt bacteria , a genetically modified with gene ( aad - 1 ) of 2,4 - D metabolizing enzyme aryloxy cotton , which has been introduced with a gene encoding alkanoate dioxygenase from Sphingobium (Sphingobium trypsin inhibitor CpTI from black - eyed pea (Vigna unguicu herbicidovorans) or with gene ( aad - 12 ) of 2,4 - D metabo lata ) , a genetically modified poplar , which has been intro lizing enzyme aryloxyalkanoate dioxygenase from Delftia duced with a gene encoding API, which is a protease (Delftia acidovorans) . Some of them are developed under 15 inhibitor protein A from arrowhead ( Sagittaria sagittifolia ) . the trade names such as Enlist® Maize, Enlist® Soybean . Examples of the insecticidal protein that confers a pest Also , there are dicamba -tolerant genetically modified soy resistance to the plants include hybrid proteins, truncated beans and cottons which have been introduced with gene proteins , and modified proteins of the insecticidal proteins (dmo ) of dicamba monooxygenase, which is dicamba described above . The hybrid proteins are produced by com metabolizing enzyme from Stenotrophomonas ( Stenotroph- 20 bining different domains of multiple insecticidal proteins omonas maltophilia strain DI- 6 ). using a common recombination technology, and Cry1Ab - Ac Examples of genetically modified plant tolerant to two or and Cry1A.105 are known. more herbicides include genetically modified cotton and Examples of the truncated proteins include Cry1Ab lack genetically modified corn , which are tolerant to both gly ing the amino acid sequence partially . Examples of the phosate and glufosinate , and marketed under the trade name 25 modified proteins include proteins in which one or more such as GlyTol® Liberty Link® , Roundup Ready® Liber amino acids of natural delta -endotoxin have been substi tyLink® Maize . Also , there are a genetically modified tuted , such as Cry1Fa2 , moCry1F , mCry3A . soybean tolerant to both glufosinate and 2,4 - D and devel Examples of other insecticidal proteins that confer insect oped under the trade name Enlist® Soybean , and a geneti resistance to plants by genetic recombination technology cally modified cotton tolerant to both glufosinate and 2,4 - D . 30 include insecticidal proteins from Bacillus cereus or Bacil A genetically modified soybean tolerant to both glyphosate lus popilliae , the insecticidal proteins Vip 1 , Vip 2 , Vip 3 of and dicamba has been developed under the trade name Btbacteria , insecticidal proteins from nematode, toxin pro Genuity® ) Roundup Ready® 2 Xtend® . Genetically modi duced by an animal such as scorpotoxin , spider toxin , bee fied corn and soybean resistant to both glyphosate and ALS venom or insect- specific neurotoxin , toxins of filamentous inhibitors have been developed under the trade name Opti- 35 fungi , plant lectin , agglutinin , protease inhibitor such as mum® GAT® . In addition , a genetically modified cotton trypsin inhibitor, serine protease inhibitor, patatin , cystatin , tolerant to both glufosinate and dicamba , a genetically papain inhibitor, ribosome inactivating protein (RIP ) such as modified corn tolerant to both glyphosate and 2,4 - D , a ricin , corn -RIP , abrin , rufin , saporin , bryodin , steroid genetically modified soybean tolerant to both glyphosate and metabolizing enzymes such as 3 -hydroxysteroid oxidase , HPPD herbicide have also been developed. Furthermore, a 40 ecdysteroid -UDP - glucosyltransferase , cholesterol oxidase , genetically modified soybean tolerant to three herbicides ecdysone inhibitor, HMG - CoA reductase , ion channel glyphosate , glufosinate and 2,4 - D has been developed . inhibitors such as sodium channel inhibitor, calcium channel Examples of the plant conferred with a pest resistance by inhibitor, juvenile hormone esterase , diuretic hormone gene recombination technology include plants conferred receptor, stilbene synthase, bibenzyl synthase , chitinase, with resistance to lepidopteran insects , coccinella insects , 45 glucanase, and the like . multipter insects , nematodes and the like . Genetically modified plants conferred with a pest resis Examples of the plant conferred with a pest resistance to tance by introducing one or more insecticidal protein gene lepidopteran insects by genetic recombination technology are known , and some of such genetically modified plants are include genetically modified plants such as soybean , cotton , commercially available . rice, poplar (Populus sp . ) , and tomato (Lycopersicon escu- 50 Examples of commercially available genetically modified lentum ), and eggplant ( Solanum melongena ), which have cotton conferred with a pest resistance include Bollgard® been introduced with a gene encoding delta - endotoxin , cotton expressing the insecticidal protein Cry1Ac of Bt which is an insecticidal protein derived from a soil bacte bacteria , Bollgard II® cotton expressing the insecticidal rium Bacillus thuringiensis bacteria (hereinafter referred to proteins Cryl Ac and Cry2Ab of Bt bacteria , Bollgard III® as Bt bacteria ). Examples of the delta - endotoxin that confers 55 expressing the insecticidal proteins Cry1Ac, Cry2Ab , Vip3A a pest resistance to lepidopteran insects include Cry1A , of Bt bacteria , VIPCOT® expressing the insecticidal pro Cry1Ab , modified Cry1Ab ( truncated Cry1Ab ) , Cry1Ac , teins Vip3A and Cry1Ac of Bt bacteria , WideStrike® Cry1Ab -Ac (hybrid protein of Cry1Ab and Cry1Ac) , expressing the insecticidal proteins Cry1Ac, Cry1F of Bt Cry1C , Cry1F, Cry1Fa2 (modified cry1F ) , moCry1F (modi bacterium . fied Cry1F ) , Cry1A . 105 (hybrid protein of Cry1Ab , Cry1Ac 60 Examples of commercially available genetically modified and Cry1F ), Cry2Ab2, Cry2Ae , Cry9C , Vip3A , Vip3Aa20 , corn conferred with a pest resistance include YieldGard® and the like . Rootworm RW expressing the insecticidal protein Cry3Bb1 Examples of the plant conferred with a pest resistance to of Bt bacteria , YieldGard Plus® expressing the insecticidal coccinella insects by genetic recombination technology proteins Cry1Ab and Cry3Bb1 of Bt bacteria , YieldGard® include genetically modified plants such as corn , potato , 65 VT Pro® expressing the insecticidal proteins Cry1A . 105 which have been introduced with a gene encoding delta and Cry2Ab2 of Bt bacteria . Agrisure® RW expressing the endotoxin , which is an insecticidal protein derived from a insecticidal protein mCry3A of Bt bacteria , Agrisure® Vip US 10,694,750 B2 11 12 tera expressing the insecticidal protein Vip3Aa20 of Bt A genetically modified canola wherein the triacylglycer bacteria , Agrisure® Duracade® expressing the insecticidal ide content, including lauric acid , has been increased by protein eCry3.1Ab of Bt bacteria are also commercially introducing a gene involved in fatty acid synthesis , 12 : 0 available . ACP thioesterase gene of laurier (Umbellularia californica ) , Examples of commercially available genetically modified 5 has been developed under the trade name Laurical® Canola . potato conferred with a pest resistance include Atlantic A genetically modified canola wherein the degradation of NewLeaf® potato , New Leaf® Russet Burbank potato , and endogenous phytic acid has been enhanced by introducing a the like, which express the insecticidal protein Cry3A of Bt gene (phyA ) of 3 -phytase , which is a degrading enzyme of bacteria . phytic acid of plants from Aspergillus niger , has been Examples of genetically modified plants conferred with 10 developed under the trade name Phytaseed® Canola . Also , resistance to plant diseases include kidney bean ( Phaseolus a genetically modified corn wherein the degradation of vulgaris ) , papaya (Carica papaya ), plum (Prunus domes endogenous phytic acid has been enhanced by introducing tica ), potato , squash ( Cucurbita pepo ), sweet pepper (Cap 3 -phytase gene (phyA ) of Aspergillus niger has been devel sicum annuum ) , tomato , and the like, which have been oped . conferred with a resistance to plant viral diseases . Specific 15 A genetically modified carnation wherein the flower color examples of genetically modified plants conferred with a has been controlled to blue by introducing a gene of dihy resistance to plant viral diseases include a genetically modi droflavonol- 4 -reductase , which is an enzyme that produces fied kidney bean which has been introduced with a gene that blue pigment delphinidin and its derivative of Petunia produces double - stranded RNA of a replication protein of (Petunia hybrida ), and a flavonoid - 3 ', 5 '- hydroxylase gene bean golden mosaic virus, a genetically modified papaya 20 from Petunia , pansy ( Viola wittrockiana ), Salvia ( Salvia which has been introduced with a coat protein gene of splendens) or carnation is known. Genetically modified papaya ringspot virus , a genetically modified potato which carnations with flower color controlled to blue have been has been introduced with a coat protein gene of potato virus developed under the trade name such as Moonldust® , Y or replication enzyme domain gene of potato leaf roll Moonshadow® , Moonshade® , Moonlite® , Moonaqua® , virus , a genetically modified squash which has been intro- 25 Moonvista® , Mooniquer , Moonpearl® , Moonberry Regis duced with a coat protein gene of Cucumber mosaic virus, tered trademark ), and Moonvelvet® . Also , genetically modi with a coat protein gene of Watermelon mosaic virus, or with fied roses with flower color controlled to blue by introducing a coat protein gene of Zucchini yellow mosaic virus, a a gene of anthocyanin - 5 -acyltransferase , which is an genetically modified sweet pepper and transgenic tomato enzyme that produces blue pigment delphinidin and its which has been introduced with a coat protein gene of 30 derivative , from Torenia ( Torenia sp . ), and a flavonoid - 3 ', Cucumber mosaic virus, and the like . 5 '- hydroxylase gene from pansy have been developed . A genetically modified potato conferred with a resistance A genetically modified corn wherein the production of to plant viral diseases is commercially available under a bioethanol has been increased by introducing a gene trade name including New Leaf® . ( Amy797E ) of heat- resistant alpha -amylase relating to Examples of the plant conferred with a resistance to plant 35 starch degradation of Thermococcales sp . have been devel disease also include plants that have been conferred with an oped under the trade name Enogen® . ability to produce a selective anti- pathogenic substance A genetically modified corn wherein the production of using genetic recombination technology. PR proteins are lysine has been increased by introducing a gene (cordapA ) known as an anti -pathogenic substance (PRPs , EP392225 ) . of dihydrodipicolinate synthase relating to the production of Such anti -pathogenic substance and genetically modified 40 amino acid lysine of Corynebacterium glutamicum has been plants that produce the same are described in EP 392225 , developed under the trade name including Mavera® . WO 199533818 , EP 353191 and the like. Examples of the A genetically modified melon and a genetically modified anti- pathogenic substance include ion channel inhibitors tomato wherein the shelf life has been improved by intro such as sodium channel inhibitors, calcium channel inhibi ducing a gene ( sam - K ) of S - adenosylmethionine hydrolase tors ( KP1, KP4, KP6 toxin produced by viruses are known ) , 45 relating to ethylene production by plant hormones from anti- pathogenic substances produced by microorganisms Escherichia coli bacteriophage T3 has been developed . such as stilbene synthase , bibenzyl synthase , chitinase , Also , genetically modified tomatoes with improved shelf life glucanase , peptide antibiotics , antibiotics having hetero by introducing a gene that lacks a part of the ACC synthase cycles , protein factors involved in plant disease resistance , gene, which is involved in the ethylene production by plant which is referred to as plant disease resistance genes and 50 hormones, from tomato ; an ACC deaminase gene from described in WO 2003000906 . Pseudomonas ( Pseudomonas chlororaphis ) that degrades Examples of genetically modified plant wherein the qual the ethylene precursor ACC , a gene that generates double ity of product has been modified includes genetically modi stranded RNA of polygalacturonase genes which degrades fied plants having a modification in lignin production , a cell wall pectin , or ACC oxidase genes of tomato related to modification in oils or fatty acid components , production of 55 the production of ethylene have been developed . A geneti phytic acid degrading enzymes , a modification in flower cally modified tomato with improved shelf life by introduc color, a modification in alpha - amylase activity , a modifica ing a gene that produces double -stranded RNA of polyga tion in amino acids, a modification in starch or carbohydrate lacturonase genes of tomato has been developed under the components , inhibition of acrylamide production , reduction trade name FLAVR SAVR® . of black spots due to mechanical damage, anti- allergy, 60 A genetically modified potato , wherein the possibility of reduction of nicotine production , or retardation of aging or decomposition of starch , formation of black spots due to grain - filling mechanical damage and production of a carcinogen (acryl There is a genetically modified alfalfa wherein the lignin amide) from heating are lowered by introducing a gene that content has been lowered by RNA interference with a gene generates double - stranded RNA of a transcription factor that generates double - stranded RNA of S -adenosyl - L -me- 65 promoting degradation of starch derived from potato , and a thionine : trans- caffeoyl CoA 3 -methyltransferase ( ccomt) gene that generates double - stranded RNA of polyphenol gene of alfalfa related to lignin production . oxidase gene and a gene that generates double -stranded US 10,694,750 B2 13 14 RNA of genes involved in asparagine production from duced with choline dehydrogenase gene (RmBetA ) of alfalfa potato , has been developed under a trade mark including Rhizobium (Rhizobium meliloti ) or E. coli ( Esherichia coli) Innate® . Also , a genetically modified potato wherein the has been developed . amylose content is lowered by introducing an antisense gene Examples of the plants wherein a trait related to growth of starch synthase from potato has been developed under the 5 and yield has been modified by genetic recombination technology include genetically modified plants having trade name Amflora . enhanced growth ability . For example , a genetically modi A genetically modified rice having alleviation effect on fied soybean which has been introduced with a gene of pollinosis with immune tolerance by introducing a gene Arabidopsis encoding a transcription factor that controls ( 7crp ) of altered antigenic protein of cedar pollen has been 10 circadian rhythm ( bbx32 ) has been developed . developed The plant according to the present invention can be a plant A genetically modified soybean wherein the oleic acid which has been modified using other techniques than genetic content is increased by introducing a partial gene ( gm - fad2 recombination technology . More specifically , it may be a 1 ) of w -6 desaturase , which is a fatty acid desaturase plant which has been conferred with tolerance to environ enzyme, of soybean to inhibit the gene expression thereof 15 mental stress , disease resistance , tolerance to herbicide , has been developed under the trade name Plenish® or insect resistance , or the like , by classical breeding technique , Treus® . Also , a genetically modified soybean wherein the genetic marker breeding technique , genome editing tech saturated fatty acid content is lowered by introducing a gene nique, or the like . ( fatb1- A ) that generates a double - stranded RNA of acyl- acyl Examples of the plantwherein a tolerance to herbicide has carrier protein -thioesterase and a gene ( fad2-1A ) that gen- 20 been conferred by classical breeding technique or genetic erates a double -stranded RNA of 8-12 desaturase has been marker breeding technique include corn , rice , wheat, sun developed under the trade name Vistive Gold® . Also , a flower (Helianthus annuus ), canola , and lentil beans ( Lens genetically modified soybean wherein the w3 fatty acid culinaris ), which are resistant to imidazolinone type ALS content is enhanced by introducing a d -6 desaturase gene inhibiting herbicides , such as imazethapyr, and are marketed (Pj.D6D ) of primrose and a 8-12 desaturase gene (Nc.Fad3 ) 25 under the trade name Clearfield® . Also , there is STS soy of Neurospora crassa has been developed . bean , which is a soybean tolerant to sulfonylurea- based A genetically modified tobacco wherein the nicotine con herbicide, as an example of plants which has been conferred tent is lowered by introducing an antisense gene of quino with a resistance to sulfonyl- based ALS -inhibiting herbi linic acid phosphoribosyltransferase (NtQPT1 ) of tobacco cides such as thifensulfuron methyl by genetic marker has been developed . 30 breeding technique . Also , there is SR corn ,which is resistant A genetically modified rice , Golden rice, introduced with to sethoxydim , as an example of plants which has been a phytoene synthase gene (psy ) of trumpet Narcissus (Nar conferred with a resistance to acetyl CoA carboxylase cissus pseudonarcissus) and a carotene desaturase gene inhibitor, such as trione oxime type hert de , aryloxyphe ( crt1 ) of soil bacteria that synthesizes carotenoids ( Erwinia noxypropionic acid type herbicide, by genetic marker breed uredovora ), which allow endosperm - specific expression to 35 ing technique. produce ß - carotene in endosperm tissue, whereby a rice Examples of the plants conferred with pest resistance by containing vitamin A is enabled to be harvested , has been classic or genetic marker breeding technique include a developed . soybean having Rag 1 (Resistance Aphid Gene 1 ) gene , Examples of the plants in which the fertile trait has been which is an aphid resistant gene . Examples of the plants modified by a genetic recombination technique include 40 conferred with resistance to nematodes by the classical genetically modified plants conferred with male sterility and breeding technique include a soybean conferred with a fertility restoration . There are genetically modified corn and resistance to Cysto nematode , and a cotton conferred with a chicory conferred with male sterility by introducing anther resistance to Root Knot nematode. tapetum cell expressing a ribonuclease gene (barnase ) of Examples of the plants which has been conferred with a Bacillus ( Bacillus amyloliquefaciens) . There is also a geneti- 45 resistance to plant disease by classic or genetic marker cally modified corn conferred with male sterility by intro breeding technique include a corn which has been conferred ducing a DNA adenine methyltransferase gene (dam ) of with a resistant to anthracnose stalk rot, a corn which has Escherichia coli. Furthermore, there is a genetically modi been conferred with a resistant to Gray leaf spot, a corn fied corn wherein the sterility has been controlled by intro which has been conferred with a resistant to Goss's wilt, a ducing alpha- amylase gene (zm - aal) of corn that confers 50 corn which has been conferred with a resistant to Fusarium male sterility and ms45 protein gene (ms45 ) of corn that stalk rot, a soybean which has been conferred with a confers fertility restoration . resistant to Asian soybean rust, a pepper which has been There is a genetically modified canola conferred with a conferred with a resistant to Phytophthora , a lettuce which fertility restoring function by introducing anther tapetum has been conferred with a resistant to powdery mildew , a cells expressing a ribonuclease inhibitory protein gene 55 tomato which has been conferred with a resistant to Bacte (barstar ) of Bacillus. In addition , there is a genetically rial wilt , a tomato which has been conferred with a resistant modified canola wherein the sterility has been controlled by to Geminivirus , and a lettuce which has been conferred with introducing a ribonuclease gene (barnase ) of Bacillus that a resistant to downy mildew . confers a male sterility and a ribonuclease inhibitory protein As an example of the plants which have been conferred gene ( barstar ) of Bacillus that confers a fertility restoration . 60 with a tolerance to dryness by classic or genetic marker Examples of the plants conferred with tolerance to envi breeding technique , a dry tolerant corn has been developed ronmental stress by a genetic recombination technique under the trade name such as Agrisure Artesian® , Optimum include genetically modified plants conferred with tolerance AQUA max® . to dryness . A dry tolerant corn which has been introduced As an example of the plants conferred with a tolerance to with a cold shock protein gene ( cspB ) of Bacillus subtilis has 65 herbicide by genomic editing technique , a canola conferred been developed under the trade name Genuity® Drought with a tolerance to sulfonylurea herbicide by rapid breed Gard® . Also , dry tolerant sugar cane which has been intro development technology wherein a mutation to confer tol US 10,694,750 B2 15 16 erance to sulfonylurea herbicide has introduced into ALS have been developed under the trade name, such as Hi- Lite gene via chimera oligonucleotides of DNA and RNA , has New Leaf® Y Potato , New Leaf® Y Russet Burbank Potato , been developed under the trade name SU canola® . and Shepody New Leaf® Y potato . As for genetically modi The above plants include a variety which has been con fied plants conferred with resistance to potato leaf roll virus ferred with two or more traits , such as tolerance to environ- 5 and pest resistance , genetically modified potatoes have been mental stress , disease resistance, tolerance to herbicide, pest developed under the trade name , such as New Leaf® Plus resistance , growth and yield traits , quality of product, and Russet Burbank Potato . sterility , using a genetic recombination technology as Examples of a variety conferred with two or more traits described above, such as a classic breeding technique , a include genetically modified plants conferred with tolerance genetic marker breeding, or a genome editing technique, as 10 well as a variety which has been conferred with two or more to herbicide and altered product quality . For example , a traits from parents by crossing the parents , which are geneti genetically modified canola and genetically modified corn , cally modified plants having same or different characteristic . which have been conferred with tolerance to glufosinate and Examples of such plant include genetically modified plants fertile trait have been developed under the trade name, such conferred with both of tolerance to herbicide and pest 15 as InVigor® Canola and InVigor® Maize , respectively . resistance . Examples of a variety conferred with two or more traits For example , as for a genetically modified plant conferred include genetically modified plants conferred with a pest with tolerance to glyphosate and pest resistance , genetically resistance and altered product quality . For example , a geneti modified cottons, such as Roundup Ready® Bollgard® cally modified corn conferred with resistance to lepidopter cotton , Roundup Ready® Bollgard II® cotton , Roundup 20 ous insects and a trait of enhanced lysine production has Ready® Flex® Bollgard II® cotton , Bollgard® III X been developed under the trade name such as Mavera® Roundup Ready® Flex® , and VIPCOT® Roundup Ready YieldGard® Maize . Flex® Cotton , have been developed . Also , genetically modi For other Examples of a variety conferred with two or fied soybeans have been developed under the trade name, more traits as mentioned above, genetically modified plants such as Agrisure® GT/ RW , Roundup Ready® YieldGard® 25 conferred with tolerance to herbicide and a trait altering maize, Genuity® VT Double Pro® , Genuity® VT Triple fertility , genetically modified plants conferred with tolerance Pro® , YieldGard® , YieldGard® CB +RW , YieldGard® VT® to herbicide and tolerance to environmental stress, geneti Rootworm® RR 2 , YieldGardORW + RR , YieldGard® VT cally modified plants conferred with tolerance to herbicide Triple , or YieldGard® Plus with RR . Furthermore, a geneti and a trait modifying growth and yield , genetically modified cally modified soybean such as Intacta® Roundup Ready® 30 plants conferred with tolerance to herbicide, pest resistance , 2 Pro has been developed . and a trait modifying product quality , genetically modified For example , as for genetically modified plants conferred plants conferred with tolerance to herbicide, pest resistance, with tolerance to glufosinate and pest resistance , genetically and tolerance to environmental stress , have been developed . modified cottons have been developed under the trade name, Examples of the plant diseases which can be controlled such as Widestrike® Cotton , Twinlink® Cotton , and Fiber- 35 according to the present invention include the followings . Max® LibertyLink® Bollgard II® . Also , genetically modi Diseases of rice: blast ( Magnaporthe oryzae ), brown spot fied corns have been developed under the trade name, such (Cochliobolus miyabeanus ), sheath blight (Rhizoctonia as Agrisure® CB /LL , Agrisure® CB /LL /RW , Agrisurer solani) , “ Bakanae” disease (Gibberella fujikuroi ), seedling Viptera®2100 , Agrisure® Viptera® 3100, Bt Xtra Maize, blight ( Pythium arrhenomanes, Pythium graminicola , NaturGard Knockout® , Herculex® RW , Herculex® CB , 40 Pythium spinosum , Pythium sp ., Rhizopus chinensis , Rhizo Herculex® XTRA , Starlink® Maize , and Liberty Link® pus oryzae, Trichoderma viride ); YieldGard® Maize . Diseases of wheats : powdery mildew (Erysiphe For example , as for genetically modified plants conferred graminis ), Fusarium blight ( Fusarium graminearum , F. ave with tolerance to glyphosate and glufosinate and pest resis nacerum , F. culmorum , F. asiaticum , Microdochium nivale ) , tance, genetically modified cottons have been developed 45 rust (Puccinia striiformis , P. graminis , P. recondite , P. hor under the trade name, such as Widestriker Roundup dei) , snow mold ( Typhula sp. , Micronectriella nivalis ) , loose Ready® Cotton , Widestrike® Roundup Ready Flex® Cot smut ( Ustilago tritici , U. nuda ) , stinking smut (Tilletia ton , Widestrike® Cotton , Registered trademark ) x Roundup caries) , eye spot (Pseudocercosporella herpotrichoides) , Ready Flex® x VIPCOT® Cotton , and Glytol® x Twin scald (Rhynchosporium secalis ), speckled leaf blotch ( Sep link® . Also , genetically modified corns have been devel- 50 toria tritici ), glume blotch (Leptosphaeria nodorum ) , net oped under the trade name, such as Agrisure® GT/ CB /LL , blotch (Pyrenophora teres Drechsler ), yellow spot (Preno Agrisure® 3000GT, Agrisure® 3122 , Agrisure® Viptera? phora tritici- repentis ), stripe (Prenophora tritici- repentis ), 3110 , Agrisure® Viptera 3111 , Agrisure® Viptera® 3220 , Rhizoctonia damping -off (Rhizoctonia damping -off ), snow Agrisure® Duracade® 5122, Agrisure® Duracade® 5222 , mold ( Typhula ishikariensis , Typhula incarnata , Sclerotinia Optimum® Intrasect, Optimum® TRIsect, Optimum® 55 borealis, Microdochium nivale ), foot lot disease (Fusarium Intrasect XTRA , Optimum® Intrasect Xtreme, Genuity® graminearum ) ; martStax® , Power Core® , Herculex® I RR , Herculex® RW Diseases of corn : smut ( Ustilago maydis ) , brown spot Roundup Ready® 2 , and Herculex XTRA® RR . ( Cochliobulus heterostrophus) , zonate leaf spot (Gloeocer For example , as for genetically modified plants conferred cospora sorghi) , southern rust (Puccinia polysora ), grey leaf with tolerance to bromoxynil and pest resistance, a geneti- 60 spot (Cercospora zaea -maydis ), Rhizoctonia damping -off cally modified cottons has been developed under the trade (Rhizoctonia solani) , Gibberella ear rot (Fusarium monili name, such as BXN® Plus Bollgard® Cotton . forme) , anthracnose ( Colletotrichum graminicola ), seedling Examples of a variety conferred with two or more traits blight (Fusarium solani, Rhizoctonia solani) ; include genetically modified plants conferred with disease Diseases of citrus: black leaf spot (Diaporthe citri) , scab resistance and pest resistance. For example , as for geneti- 65 (Elsinoe fawcetti ), fruit rot (Penicillium digitatum , P. itali cally modified plants conferred with resistance to potato cum ); brown rot (Phytophthora parasitica , Phytophthora virus Y and pest resistance , genetically modified potatoes citrophthora ) ; US 10,694,750 B2 17 18 Diseases of apple : Monilia leaf blight (Monilinia mali ), (Phaeoisariopsis griseola ), Rhizoctonia root necrosis Valsa canker ( Valsa ceratosperma) , powdery mildew (Po (Rhizoctonia solani) , Aphanomyces root necrosis (Aphano dosphaera leucotricha ), Alternaria blotch ( Alternaria alter myces euteiches ); nate apple pathotype ) , scab ( Venturia inaequalis ) , anthra Diseases of peanut : leaf spot ( Cercospora personata ), cnose (Colletotrichum gloeosporioies, Colletotrichum 5 brown( Sclerotium leaf spotrolfsii (Cercospora ) ; arachidicola ), southern blight acutatum ), Phytophthora rot (Phytophtora cactorum ) ; Diseases of pea: powdery mildew ( Erysiphe pisi ), root blotch (Diplocarpon mali ); ring rot ( Botryosphaeria beren necrosis ( Fusarium solani f. Sp . Pisi ) ; geriana) ; Diseases of potato : early blight ( Alternaria solani) , late Diseases of pear: scab (Venturia nashicola , V. pirina ), blight (Phytophthora infestans ), powdery scab (Spon black spot (Alternaria alternate Japanese pear pathotype ) , 10 gospora subterranea ), pink rot (Phytophthora erythrosep rust (Gymnosporangium haraeanum ) , Phytophthora fruit rot tica ) ; ( Phytophthora cactorum ) ; Diseases of strawberry : powdery mildew ( Sphaerotheca Diseases of peach : brown rot (Monilinia fructicola ), scab humuli) , anthracnose (Glomerella cingulata ) ; ( Cladosporium carpophilum ), Phomopsis seed decay (Pho- 15 tumDiseases ), white ofscab tea ( : Elsinoenet blister leucospila blight )(Exobasidium, gray blight (reticula Pestalo mopsis sp .) ; tiopsis sp .) , anthracnose ( Colletotrichum theae -sinensis ); Diseases of grape: anthracnose (Elsinoe ampelina ), ripe Diseases of cotton : Fusarium wilt (Fusarium oxysporum ), rot ( Colletorichum gloeosporioides , Colletotrichum acuta Fusarium wilt (Rhizoctonia solani) ; tum ), powdery mildew (Uncinula necator ), rust ( Phakop Diseases of tobacco : brown spot ( Alternaria longipes ), sora ampelopsidis) , black rot (Guignardia bidwellii) ,downy 20 powdery mildew ( Erysiphe cichoracearum ), anthracnose mildew ( Plasmopara viticola ) , gray mold (Botrytis cinerea ) ; ( Colletotrichum tabacum ) , downy mildew (Peronospora Diseases of persimmon : anthracnose (Gloeosporium tabacina ), black shank (Phytophthora nicotianae ) ; kaki) , leaf spot (Cercospora kaki, Rycosphaerella nawae ); Diseases of sugar beet: brown leaf spot ( Cercospora Diseases of cucumbers : anthracnose (Colletotrichum beticola ), leaf blight (Thanatephorus cucumeris ), root orbiculare ), powdery mildew ( Sphaerotheca fuliginea ), 25 (necrosis Aphanomyces (Thanatephorus cochlioides cucumeris ) ; ), Aphanomyces root rot gummy stem blight (Mycosphaerella melonis ) , Fusarium Diseases of rose: scab (Diplocarpon rosae ), powdery wilt (Fusarium oxysporum ), downy mildew ( Pseudoperono mildew (Sphaerotheca pannosa ), downy mildew ( Perono spora cubensis) , Phytophthora blight ( Phytophthora sp .) , spora sparsa ) ; damping -off (Pythium sp .) ; Rhizoctonia damping -off Diseases of chrysanthemum : brown leaf spot (Septoria ( Rhizoctonia solani) ; 30 chrysanthemi- indici) , rust (Septoria chrysanthemi- indici ), Diseases of tomato : Early blight ( Alternaria solani) , Leaf downy mildew (Bremia lactucae ) ; mold (Cladosporium fulvum ), late blight ( Phytophthora Diseases of radish : Alternaria leaf spot (Alternaria bras infestans) , leaf spot ( Stemphylium lycopesici ) ; sicicola ) ; Diseases of eggplant: brown spot (Phomopsis vexans ), 35 carpaDisease ), brown of turfgrasspatch and : dollarlarge patch spot (Rhizoctonia (Sclerotinia solanihomeo) ; powdery mildew ( Erysiphe cichoracearum ); Diseases of banana: Sigatoka disease (Mycosphaerella Diseases of Brassica vegetables: Alternaria leaf spot fijiensis , Mycosphaerella musicola , Pseudocercospora (Alternaria japonica ) , leaf spot (Cercosporella brassicae) , musae ) ; Clubroot ( Plasmodiophora brassicae ) , downy mildew (Per Diseases of sunflower : downy mildew ( Plasmopara hal onospora parasitica ), root rot (Phoma lingam ) ; 40 stedii) , Alternaria leaf spot ( Alternaria helianthi) , southern Diseases of rapeseed : Sclerotinia rot (Sclerotinia sclero blight ( Sclerotium rolfsii) , damping -off ( Rhizoctonia solani) , tiorum ), Alternaria leaf spot (Alternaria brassicae) , pow Sclerotinia rot (Sclerotinia sclerotiorum ), rust (Puccinia dery mildew ( Erysiphe cichoracearum ), black leg (Lep helianthi) ; tosphaeria maculans ), Rhizoctonia damping -off Diseases of various plants : diseases caused by Pythium ( Rhizoctonia solani) ; 45 spp . (Pythium aphanidermatum , Pythium debarianum , Diseases of green onion : rust (Puccinia allii ), Fusarium Pythium graminicola , Pythium irregulare , pythium ulti wilt ( Fusarium oxysoporum ) ; mum ) , gray mold ( Botrytis cinerea ), Sclerotinia rot (Sclero Diseases of onion : gray -mold neck rot (Botrytis allii ), leaf tinia sclerotiorum ); damping -off (Rhizoctonia solani) . blight (Botrytis squamosa ) , Fusarium basal rot ( Fusarium The present invention can be applied preferably to plant oxysoporum , Fusarium solani) ; 50 diseases caused by bacteria in the genus of Rhizoctonia spp ., Diseases of soybean : purple stain ( Cercospora kikuchii ), Fusarium spp ., Pythium spp ., Phoma spp ., and Penicillium anthracnose (Elsinoe glycines ), pod and stem blight ( Dia porthe phaseolorum var. Sojae ) , brown spot ( Septoria gly spp . cines ), leaf spot (Cercospora sojina ), rust ( Phakopsora EXAMPLES pachyrhizi ), Fusarium blight (Phytophthora sojae) , damp- 55 ing -off ( Rhizoctonia solani) , root necrosis (Rhizoctonia The invention is described in more detail with reference solani) , Fusarium root necrosis (Fusarium solani) , anthra to the following Preparation Examples , Formulation cnose ( Colletotrichum truncatum ) , Fusarium blight Examples , Seed Treatment Examples , and Test Examples, ( Fusarium oxysporum , F. avenaceum , F. roseum ), Sclero which are not intended to limit the scope of the present tinia rot (Sclerotinia sclerotiorum ); 60 invention . The term “ part ” means “ part by weight ” unless Diseases of adzuki bean : gray mold ( Botrytis cinerea ), otherwise specified . Sclerotinia rot ( Sclerotinia sclerotiorum ), rust (Uromyces Preparation Examples are provided below . phaseoli ) , anthracnose (Coletotrichum phaseolorum ) ; Diseases of kidney bean : gray mold ( Botrytis cinerea ) , Preparation Example 1 Sclerotinia rot (Sclerotinia sclerotiorum ), anthracnose (Col- 65 letotrichum lindemthianum ), Fusarium wilt ( Fusarium A culture broth of the present bacterial strain , which has oxysporum ), rust (Uromyces phaseoli ), angular leaf spot been cultured by a known technique , is centrifuged accord US 10,694,750 B2 19 20 ing to an ordinary method to separate into a supernatant and 1900xg for 10 min to separate into a supernatant and a a precipitate . The supernatant is removed , and the precipitate precipitate . After removing the supernatant, the precipitate is washed with sterilized water to obtain a bacterial mass . was washed with sterilized water and centrifuged at 1900xg The obtained bacterial mass is suspended in water , dried on for 10 min . The supernatantwas removed to obtain 3.8x1011 spray drier , and the resultant dried product is pulverized to 5 cfu / g of bacterial cells of the present bacterial strain . obtain a powder of the present bacterial strain . Preparation Example 7 Preparation Example 2 The bacterial cells of the present bacterial strain obtained A culture broth of the present bacterial strain , which has 10 as described in Preparation Example 6 were frozen at -80 ° been cultured by a known technique, is frozen at -80 ° C., C. and freeze -dried . The dried product obtained thus by freeze -dried and pulverized to obtain a powder of the present freeze- drying was pulverized using scoopula to obtain 2.8x bacterial strain . 1012 cfu /g of a powder of the present bacterial strain . Formulation Examples are provided below . Preparation Example 3 15 Formulation Example 1 In a 500 mL Erlenmeyer flask with baffle , a platinum loop scraping of the present bacterial strain , which have been To a mixture containing 1 part of metconazole , 5 parts of cultured in TSA (an agar medium containing 15 g/ L of white carbon , 8 parts of sodium lignin sulfonate , 2 parts of casein peptone, 5 g /L of soybean peptone, 5 g/ L of sodium 20 sodium alkyl naphthalene sulfonate are added a powder of chloride , and 15 g /L of agar ), are inoculated to a liquid the present bacterial strain obtained as described in Prepa medium containing 200 mL TSB ( a liquid medium contain ration Example 1 or 2 , in an amount of 1x1010 cfu per 1 g ing 17 g/ L of casein peptone, 3 g /L of soybean peptone , 2.5 of the formulation , and diatomaceous earth to 100 parts , K2HPO4g / L of glucose) and incubated, 5 g / L of at sodium 30° C. chloridefor 12 hours and to2.5 24 g hours /L of 25 followed bymixing and grinding to obtain wettable powder. to obtain a liquid culture . In a 500 mL volume Erlenmeyer flask with baffle , 2 mL of the liquid culture is inoculated to Formulation Example 2 200 mL of a fresh TSB and cultured with shaking for 24 To a mixture containing 1 parts of tebuconazole , 5 parts bacterialhours to strain48 hours ( hereinafter to obtain referreda liquid to culture as Liquid of theCulture present a ). 30 of white carbon , 8 parts of sodium lignin sulfonate , 2 parts The Liquid Culture a is centrifuged according to a conven of sodium alkyl naphthalene sulfonate are added a powder of tional manner to separate into a supernatant and precipitate . the present bacterial strain obtained as described in Prepa After removing the supernatant, the precipitate is washed ration Example 1 or 2, in an amount of 1x1010 cfu per g with sterile water and centrifuged . The supernatant is of the formulation , and diatomaceous earth to 100 parts , removed to obtain bacterial cells of the present bacterial 35 followed by mixing and grinding to obtain wettable powder . strain . Formulation Example 3 Preparation Example 4 To a mixture containing 2 parts of prothioconazole , 5 The bacterial cells of the present bacterial strain obtained 40 parts of white carbon , 8 parts of sodium lignin sulfonate , 2 in Preparation Example 3 are suspended in water, dried on parts of sodium alkyl naphthalene sulfonate are added a spray drier , and pulverized the resulting dried product to powder of the present bacterial strain obtained as described obtain a powder of the present bacterial strain . in Preparation Example 1 or 2 , in an amount of 1x1010 cfu per 1 g of the formulation , and diatomaceous earth to 100 Preparation Example 5 45 parts , followed by mixing and grinding to obtain wettable powder . The Liquid Culture a is obtained as described in Prepa ration 3. The Liquid Culture a is frozen at -80 ° C., and Formulation Example 4 freeze- dried and pulverized to obtain a powder of the present bacterial strain . 50 To a mixture containing 2 parts of ipconazole , 5 parts of white carbon , 8 parts of sodium lignin sulfonate , 2 parts of Preparation Example 6 sodium alkyl naphthalene sulfonate are added a powder of the present bacterial strain obtained as described in Prepa In a Erlenmeyer flask with baffle , a platinum loop scrap ration Example 1 or 2 , in an amount of 1x1010 cfu per 11 g ing of the present bacterial strain , which have been cultured 55 of the formulation , and diatomaceous earth to 100 parts , in TSA (an agar medium containing 15 g / L of casein followed by mixing and grinding to obtain wettable powder . peptone , 5 g /L of soybean peptone, 5 g /L of sodium chloride , and 15 g / L of agar ), were inoculated to a liquid medium Formulation Example 5 containing 200 mL TSB ( a liquid medium containing 17 g / L of casein peptone, 3 g / L of soybean peptone, 2.5 g /L of 60 To a mixture containing 6 parts of difenoconazole and 5 glucose, 5 g / L of sodium chloride and 2.5 g / L of K2HPO4) parts of white carbon , 8 parts of sodium lignin sulfonate , 2 and incubated at 30 ° C. for 23 hours to obtain a liquid parts of sodium alkyl naphthalene sulfonate are added a culture . The liquid culture (2 % (v / v )) was inoculated to a powder of the present bacterial strain obtained as described fresh TSB in a Erlenmeyer flask with baffle and cultured at in Preparation Example 1 or 2 , in an amount of 1x1010 cfu 30 ° C. with shaking for 43 hours to obtain a liquid culture 65 per 1 g of the formulation , and diatomaceous earth to 100 of the present bacterial strain (hereinafter referred to as parts , followed by mixing and grinding to obtain wettable Liquid Culture b ). The liquid culture b was centrifuged at powder. US 10,694,750 B2 21 22 Formulation Example 6 oxyethylene alkyl ether sulfate ammonium salt are added a powder of the present bacterial strain obtained as described To a mixture containing 2 parts of triticonazole and 5 parts in Preparation Example 1 or 2 , in an amount of 1x1010 cfu of white carbon , 8 parts of sodium lignin sulfonate , 2 parts per 1 g of the formulation , and water to 100 parts are added ofthe sodium present alkyl bacterialnaphthalene strain obtained sulfonate as are described added a powderin Prepa of 5 and finely milled by wet- milling to obtain a flowable for ration Example 1 or 2 , in an amount of 1x1010 cfu per 1 mulation . of the formulation , and diatomaceous earth to 100 parts , followed by mixing and grinding to obtain wettable powder . Formulation Example 13 Formulation Example 7 10 To a mixture containing 30 parts of difenoconazole and parts of white carbon containing 30 % by weight of poly To a mixture containing 6 parts of triadimenol and 5 parts oxyethylene alkyl ether sulfate ammonium salt are added a of white carbon , 8 parts of sodium lignin sulfonate , 2 parts powder of the present bacterial strain obtained as described of sodium alkyl naphthalene sulfonate are added a powder of in Preparation Example 1 or 2 , in an amount of 1x1010 cfu the present bacterial strain obtained as described in Prepa 15 per 1 g of the formulation , and water to 100 parts , followed ration Example 1 or 2 , in an amount of 1x1010 cfu per 1 g by wet- milling to finely milled to obtain a flowable formu of the formulation , and diatomaceous earth to 100 parts , lation . followed by mixing and grinding to obtain wettable powder. Formulation Example 14 Formulation Example 8 20 To a mixture containing 10 parts of triticonazole and 30 To a mixture containing 2 parts of bromconazole and 5 parts of white carbon containing 30 % by weight of poly parts of white carbon , 8 parts of sodium lignin sulfonate , 2 oxyethylene alkyl ether sulfate ammonium salt are added a parts of sodium alkyl naphthalene sulfonate are added a powder of the present bacterial strain obtained as described powder of the present bacterial strain obtained as described 25 in Preparation Example 1 or 2 , in an amount of 1x1010 cfu in Preparation Example 1 or 2 , in an amount of 1x1010 cfu per 1 g of the formulation , and water to 100 parts , followed per 1 g of the formulation , and diatomaceous earth to 100 by wet -milling to finely milled to obtain a flowable formu parts , followed by mixing and grinding to obtain wettable lation . powder 30 Formulation Example 15 Formulation Example 9 To a mixture containing 25 parts of triadimenol and 30 To a mixture containing 5 parts of metconazole and 30 parts of white carbon containing 30 % by weight of poly parts of white carbon containing 30 % by weight of poly oxyethylene alkyl ether sulfate ammonium salt are added a oxyethylene alkyl ether sulfate ammonium salt are added a 35 powder of the present bacterial strain obtained as described powder of the present bacterial strain obtained as described in Preparation Example 1 or 2 , in an amount of 1x1010 cfu in Preparation Example 1 or 2 , in an amount of 1x1010 cfu per 11 g of the formulation , and water to 100 parts , followed per 11 g of the formulation , and water to 100 parts , followed by wet -milling to finely milled to obtain a flowable formu by wet -milling to finely milled to obtain a flowable formu lation . lation . 40 Formulation Example 16 Formulation Example 10 To a mixture containing 10 parts of bromconazole and 30 To a mixture containing 5 parts of tebuconazole and 30 parts of white carbon containing 30 % by weight of poly parts of white carbon containing 30 % by weight of poly- 45 oxyethylene alkyl ether sulfate ammonium salt are added a oxyethylene alkyl ether sulfate ammonium salt are added a powder of the present bacterial strain obtained as described powder of the present bacterial strain obtained as described in Preparation Example 1 or 2 , in an amount of 1x1010 cfu in Preparation Example 1 or 2 , in an amount of 1x1010 cfu per 1 g of the formulation , and water to 100 parts, followed per 1 g of the formulation , and water to 100 parts , followed by wet- milling to finely milled to obtain a flowable formu by wet -milling to finely milled to obtain a flowable formu- 50 lation . lation . Formulation Example 17 Formulation Example 11 To a mixture containing 5 parts of white carbon , 8 parts To a mixture containing 10 parts of prothioconazole and 55 of sodium lignin sulfonate , and 2 parts of sodium alkyl parts of white carbon containing 30 % by weight of poly naphthalene sulfonate are added the bacterial cells or pow oxyethylene alkyl ether sulfate ammonium salt are added a der of the present bacterial strain obtained as described in powder of the present bacterial strain obtained as described any one of Preparation Examples 3 to 5 , in an amount of in Preparation Example 1 or 2 , in an amount of 1x1010 cfu 1x1010 cfu per 1 g of the formulation , and diatomaceous per 1 g of the formulation , and water to 100 parts , followed 60 earth to 100 parts to obtain a mixture. The mixture was by wet -milling to finely milled to obtain a flowable formu milled to obtain wettable powder. lation . Formulation Example 18 Formulation Example 12 65 To 30 parts of white carbon containing 30 % by weight of To a mixture containing 10 parts of ipconazole and 30 polyoxyethylene alkyl ether sulfate ammonium salt are parts of white carbon containing 30 % by weight of poly added the bacterial cells or powder of the present bacterial US 10,694,750 B2 23 24 strain obtained as described in any one of Preparation acetone/ Tween 20 (weight ratio = 95 : 5 ) and diluting with Examples 3 to 5 , in an amount of 1x1010 cfu or 1x1012 cfu water in an amount of 0.2 g of prothioconazole per 1 kg of per 1 g of the formulation , and water to 100 parts to obtain the sorghum seeds. The Sorghum seeds thus treated with a mixture . The mixture was finely milled by wet- milling to prothioconazole are treated by smearing treatment with a obtain a flowable formulation . 5 flowable formulation prepared as described in Seed Treat ment Example 3 in an amount of 1x1010 cfu of the present Formulation Example 19 bacterial strain per 1 kg of said sorghum seeds . To 30 parts of white carbon containing 30 % by weight of polyoxyethylene alkyl ether sulfate ammonium salt were Seed Treatment Example 5 added the powder of the present bacterial strain obtained in 10 Preparation Example 7 , in an amount of 1x1010 cfu or Wheat seeds are treated by smearing treatment with a 1x1012 cfu per 1 g of the formulation , and water to 100 parts liquid mixture containing the flowable formulation of the to obtain a mixture. The mixture was finely milled by present bacterial strain and triadimenol flowable formulation wet -milling to obtain a flowable formulation . (30 % flowable formulation , trade name: Baytan 30 , Bayer Seed Treatment Examples are provided below . 15 CropScience) , in an amount of 1x1010 cfu of the present bacterial strain and 1.0 g of triadimenol per 1 kg of the wheat Seed Treatment Example 1 seeds . To a mixture containing 5 parts of white carbon , 8 parts of sodium lignin sulfonate and 2 parts of sodium alkyl 20 Seed Treatment Example 6 naphthalene sulfonate are added a powder of the present Wheat seeds are treated by smearing treatment with the bacterial strain obtained as described in Preparation flowable formulation of the present bacterial strain and Example 1 , in an amount of 1x1010 cfu per 1 g of the difenoconazole prepared as described in Formulation obtained formulation , and diatomaceous earth to 100 parts , Example 13 , in an amount of 2x1010 cfu of the present offollowed the present by mixing bacterial and straingrinding . to obtain wettable powder 25 bacterial strain and 0.6 g of difenoconazole per 1 kg of the Wheat seeds are treated by smearing treatment with wheat seeds . metconazole flowable formulation (40.0 % flowable formu lation , trade name: Metolock Fungicide , Valent U.S.A. Cor Seed Treatment Example 7 poration ) in an amount of 0.1 g ofmetconazole per 1 kg of the wheat seeds. The wheat seeds thus treated with metcon 30 Wheat seeds are treated by smearing treatment with the azole are treated by wet powder coating with wettable flowable formulation of the present bacterial strain and powder of the present bacterial strain obtained by the triticonazole prepared as described in Formulation Example process descried above in an amount of 1x1010 cfu /kg of the 14 , in an amount of 2x1010 cfu of the present bacterial strain present bacterial strain per 1 kg of said wheat seeds . 35 and 0.2 g of triticonazole per 1 kg of the wheat seeds. Seed Treatment Example 2 Seed Treatment Example 8 Soybean seeds are treated by smearing treatment with a Wheat seeds are treated by smearing treatment with the chemical liquid prepared by dissolving ipconazole in flowable formulation of the present bacterial strain and wateracetone in/ Tweenan amount 20 ( weightof 0.2 g ratioof ipconazole = 95 :5 ) and per diluting 1 kg ofwith the 40 bromconazole prepared as described in Formulation seeds. The soybean seeds thus treated with ipconazole are Example 16 , in an amount of 2x1010 cfu of the present treated by wet powder coating with wettable powder of the bacterial strain and 0.2 g of bromconazole per 1 kg of the present bacterial strain prepared as described in Seed Treat wheat seeds. ment Example 1 in an amount of 1x1010 cfu /kg of the present bacterial strain per 1 kg of said soybean seeds. 45 Seed Treatment Example 9 Seed Treatment Example 3 Corn seeds are treated by smearing treatment with a liquid mixture containing a flowable formulation of the present To 30 parts of white carbon containing 30 % by weight of bacterial strain obtained as described in Formulation polyoxyethylene alkyl ether sulfate ammonium salt are 50 Example and tebuconazole flowable formulation ( 28.3 % added a powder of the present bacterial strain obtained as flowable formulation , trade name: Raxil 2.6F , Bayer Crop described in Preparation Example 2 , in an amount of 1x1010 Science ), in an amount of 1x1010 cfu of the present bacterial cfu per 1 g of the formulation , and water to 100 parts , and strain and 1.3 g of tebuconazole per 1 kg of the corn seeds . the mixture is finely milled by wet -milling to obtain a Test Examples are provided below . flowable formulation of the present bacterial strain . 55 Corn seeds are treated by smearing treatmentwith a liquid Test Example 1 mixture containing the flowable formulation of the present bacterial strain ( 1x1010 cfu per 1 kg of the soybean seeds ) In a rotary seed treatment machine ( trade name: HEGE11 , and tebuconazole flowable formulation ( 28.3 % flowable manufactured by WINTERSTEIGER ) , corn seeds ( variety : formulation , trade name: Raxil 2.6F, Bayer CropScience ) , in 60 yellow dent corn ) are treated by smearing treatment with a an amount of 1.3 g of tebuconazole per 1 kg of the corn liquid wherein a flowable formulation of the present bacte seeds . rial strain as prepared in the Seed Treatment Example 3 (adjusted to 1x1010 cfu per 1 kg of the corn seeds) was Seed Treatment Example 4 mixed with metconazole flowable formulation (40.0 % flow 65 able formulation , trade name: Metolock Fungicide, Valent Sorghum seeds are treated by smearing treatment with a U.S.A. Corporation , adjusted to 0.1 g ofmetconazole per 1 chemical liquid prepared by dissolving prothioconazole in kg of the corn seeds ), or with tebuconazole flowable for US 10,694,750 B2 25 26 mulation ( 28.3 % flowable formulation , trade name: Raxil tured in a bran medium . Cultivation is carried out in a 2.6F , Bayer CropScience , adjusted to 0.1 g of tebuconazole greenhouse under irrigation (“ treated compartment" ) . The per 1 kg of the corn seeds ), or with triadimenol flowable plants are investigated 20 days after for the number of formulation (30 % flowable formulation , trade name: Baytan diseased plants , and the disease incidence is calculated by 30 , Bayer CropScience , adjusted to 1.0 g of triadimenol per 5 the following “Equation 1 ” . Using untreated seeds, seeding , 1 kg of the corn seeds) . covering with a soil and cultivation are conducted in a A plastic pot is filled with a soil , and then , the seeds thus similar manner as described above for " treated compart treated are seeded and covered with a soil, which has been ment” ( “ untreated compartment” ) . The plants are investi mixed with damping -off fungus ( Rhizoctonia solani) cul gated 20 days after for the number of diseased plants , and the tured in a bran medium . Cultivation is carried out in a 10 disease incidence is calculated by the following “ Equation greenhouse under irrigation (“ treated compartment” ) . The 1 ” . Based on the disease incidences of the treated compart plants are investigated 20 days after for the number of ment and the untreated compartment, the control value of the diseased plants , and the disease incidence is calculated by treated compartment is calculated by the following equation the following “ Equation 1” . Using untreated seeds, seeding , “ Equation 2” , and the treated compartment is confirmed to covering with a soil and cultivation are conducted in a 15 have a good control effect on plant disease . similar manner as described above for " treated compart ment” (“ untreated compartment” ) . The plants are investi Disease incidence ( % )= 100x (number of diseased gated 20 days after for the number of diseased plants , and the plant/ total number of seeded seeds ) Equation 1 disease incidence is calculated by the following “ Equation Control value ( % ) = 100x [ (disease incidence in 1 ” . Based on the disease incidences of the treated compart- 20 untreated compartment - disease incidence in ment and the untreated compartment, the control value of the treated compartment )/ disease incidence in treated compartment is calculated by the following equation untreated compartment] Equation 2 “ Equation 2” , and the treated compartment is confirmed to The composition of the invention shows a significantly have a good control effect on plant disease . higher controlling effect. 25 Disease incidence % ) = 100x (number of diseased plant/ total number of seeded seeds ) Equation 1 Test Example 3 Control value ( % ) = 100x [ (disease incidence in Soybean seeds ( variety :“ Hatayutaka ” ) are treated by wet untreated compartment - disease incidence in powder coating treatment with the wettable powder of the treated compartment) /disease incidence in 30 present bacterial strain and ipconazole prepared in Formu untreated compartment] Equation 2 lation Example 4 ( adjusted to 1x1011 cfu of the present The composition of the invention shows a significantly bacterial strain and 0.2 g of ipconazole per 1 kg of the higher controlling effect . soybean seeds ) . A plastic pot is filled with a soil, and then , the seeds thus Test Example 2 35 treated are seeded and covered with a soil, which has been mixed with damping -off fungus ( Rhizoctonia solani) cul Corn seeds ( variety : yellow dent corn ) are treated by wet tured in a bran medium . Cultivation is carried out in a powder coating treatment with the wettable powder of the greenhouse under irrigation (“ treated compartment” ) . The present bacterial strain and metconazole prepared in For plants are investigated 20 days after for the number of mulation Example 1 ( adjusted to 1x1011 cfu of the present 40 diseased plants , and the disease incidence is calculated by bacterial strain and 0.1 g ofmetconazole per 1 kg of the corn the following “ Equation 1 ” . Using untreated soybean seeds , seeds ), or with the wettable powder of the present bacterial seeding , covering with a soil and cultivation are conducted strain and tebuconazole prepared in Formulation Example 2 in a similar manner as described above for " treated com (adjusted to 1x1011 cfu of the present bacterial strain and 0.1 partment” (“ untreated compartment” ) . The plants are inves g of tebuconazole per 1 kg of the corn seeds) , or with the 45 tigated 20 days after for the number of diseased plants , and wettable powder of the present bacterial strain and prothio the disease incidence is calculated by the following “ Equa conazole prepared in Formulation Example 3 (adjusted to tion 1 ” . Based on the disease incidences of the treated 1x1011 cfu of the present bacterial strain and 0.2 g of compartment and the untreated compartment , the control prothioconazole per 1 kg of the corn seeds) , or with the value of the treated compartment is calculated by the fol wettable powder of the present bacterial strain and difeno- 50 lowing equation “ Equation 2 ” , and the treated compartment conazole prepared in Formulation Example 5 (adjusted to is confirmed to have a good control effect on plant disease . 1x1011 cfu of the present bacterial strain and 0.6 g of difenoconazole per 1 kg of the corn seeds) , or with the Disease incidence ( % ) = 100x (number of diseased wettable powder of the present bacterial strain and triticon plant/ total number of seeded seeds ) Equation 1 azole prepared in Formulation Example 6 (adjusted to 55 Control value ( % ) = 100x [( disease incidence in 1x1011 cfu of the present bacterial strain and 0.2 g of untreated compartment - disease incidence in triticonazole per 1 kg of the corn seeds) , or with the wettable treated compartment ) /disease incidence in powder of the present bacterial strain and triadimenol pre untreated compartment] Equation 2 pared in Formulation Example 7 ( adjusted to 1x1011 cfu of The composition of the invention shows a significantly the present bacterial strain and 1.0 g of triadimenol per 1 kg 60 higher controlling effect. of the corn seeds ), or with the wettable powder of the present bacterial strain and bromconazole prepared in Formulation Test Example 4 Example 8 (adjusted to 1x1011 cfu of the present bacterial strain and 0.2 g of bromconazole per 1 kg of the corn seeds ) . In a rotary seed treatmentmachine ( trade name: HEGE11, A plastic pot is filled with a soil, and then , the seeds thus 65 manufactured by WINTERSTEIGER ), corn seeds ( variety : treated are seeded and covered with a soil , which has been yellow dent corn ) are treated by smearing treatment with the mixed with damping -off fungus (Rhizoctonia solani) cul flowable formulation of the present bacterial strain and US 10,694,750 B2 27 28 metconazole prepared in Formulation Example 9 ( adjusted greenhouse under irrigation ( “ treated compartment” ) . The to 2x1010 cfu of the present bacterial strain and 0.1 g of plants are investigated 20 days after for the number of metconazole per 1 kg of the corn seeds) , or with the flowable diseased plants , and the disease incidence is calculated by formulation of the present bacterial strain and tebuconazole the following " Equation 1 " . Using untreated soybean seeds, prepared in Formulation Example 10 (adjusted to 2x1010 cfu 5 seeding, covering with a soil and cultivation are conducted of the present bacterial strain and 0.1 g of tebuconazole per in a similar manner as described above for " treated com 1 kg of the corn seeds ), or with the flowable formulation of partment” (“ untreated compartment” ) . The plants are inves the present bacterial strain and prothioconazole prepared in tigated 20 days after for the number of diseased plants , and Formulation Example 11 (adjusted to 2x1010 cfu of the the disease incidence is calculated by the following “Equa present bacterial strain and 0.2 g of prothioconazole per 1 kg 10 tion 1” . Based on the disease incidences of the treated of the corn seeds) , or with the flowable formulation of the compartment and the untreated compartment , the control present bacterial strain and difenoconazole prepared in For value of the treated compartment is calculated by the fol mulation Example 13 ( adjusted to 2x100 cfu of the present lowing equation “ Equation 2 ” , and the treated compartment bacterial strain and 0.6 g of difenoconazole per 1 kg of the is confirmed to have a good control effect on plant disease . corn seeds ) , or with the flowable formulation of the present 15 bacterial strain and triticonazole prepared in Formulation Disease incidence ( % ) = 100x (number of ungermi Example 14 (adjusted to 2x1010 cfu of the present bacterial nated seeds/ total number of seeded seeds ) Equation 1 strain and 0.2 g of triticonazole per 1 kg of the corn seeds ), Control value ( % ) = 100x [ (disease incidence in or with the flowable formulation of the present bacterial untreated compartment - disease incidence in strain and triadimenol prepared in Formulation Example 15 20 treated compartment) /disease incidence in (adjusted to 4x1010 cfu of the present bacterial strain and 1.0 untreated compartment ] Equation 2 g of triadimenol per 1 kg of the corn seeds ), or with the The composition of the invention shows a significantly flowable formulation of the present bacterial strain and higher controlling effect. bromconazole prepared in Formulation Example 16 ( ad justed to 2x101 ° cfu of the present bacterial strain and 0.2 g 25 Test Example 6 of bromconazole per 1 kg of the corn seeds) . A plastic pot is filled with a soil, and then , the seeds thus In a rotary seed treatment machine ( trade name: HEGE11, treated are seeded and covered with a soil , which has been manufactured by WINTERSTEIGER ), corn seeds (variety : mixed with damping - off fungus (Rhizoctonia solani) cul yellow dent corn ) are treated by smearing treatment inde tured in a bran medium . Cultivation is carried out in a 30 pendently with metconazole flowable formulation ( 40.0 % greenhouse under irrigation ( “ treated compartment" ) . The flowable formulation , trade name: Metolock Fungicide , plants are investigated 20 days after for the number of Valent U.S.A. Corporation , adjusted to 0.1 g ofmetconazole diseased plants , and the disease incidence is calculated by per 1 kg of the corn seeds ), or with tebuconazole flowable the following " Equation 1 ”. Using untreated seeds , seeding , formulation (28.3 % flowable formulation , trade name: Raxil covering with a soil and cultivation are conducted in a 35 2.6F , Bayer CropScience , adjusted to 0.1 g of tebuconazole similar manner as described above for “ treated compart per 1 kg of the corn seeds ), or with a chemical liquid ment” (“ untreated compartment” ) . The plants are investi prepared by dissolving prothioconazole in acetone/ Tween gated 20 days after for the number of diseased plants , and the 20 (weight ratio = 95 :5 ) and diluting with water ( adjusted to disease incidence is calculated by the following “ Equation 0.2 g of prothioconazole per 1 kg of the corn seeds) , or with 1 ” . Based on the disease incidences of the treated compart- 40 a chemical liquid prepared by dissolving difenoconazole in ment and the untreated compartment, the control value of the acetone/ Tween 20 (weight ratio = 95 : 5 ) and diluting with treated compartment is calculated by the following equation water (adjusted to 0.6 g of difenoconazole per 1 kg of the “ Equation 2 ” , and the treated compartment is confirmed to corn seeds ) , or with a chemical liquid prepared by dissolving have a good control effect on plant disease . triticonazole in acetone/ Tween 20 ( weight ratio = 95 :5 ) and Disease incidence ( % ) = 100x ( number of diseased 45 diluting with water ( adjusted to 0.2 g of triticonazole per 1 Equation 1 kg of the corn seeds ), or with triadimenol flowable formu plant/ total number of seeded seeds ) lation ( 30 % flowable formulation , trade name: Baytan 30 , Control value ( % ) = 100x [ (disease incidence in Bayer CropScience , adjusted to 1.0 g of triadimenol per 1 kg untreated compartment- disease incidence in of the corn seeds) , or with a chemical liquid prepared by treated compartment) /disease incidence in 50 dissolving bromconazole in acetone/ Tween 20 (weight untreated compartment] Equation 2 ratio = 95 : 5 ) and diluting with water ( adjusted to 0.2 g of The composition of the invention shows a significantly bromconazole per 1 kg of the corn seeds) . The seeds thus higher controlling effect. treated with metconazole , tebuconazole , prothioconazole , difenoconazole , triticonazole , triadimenol or bromconazole Test Example 5 55 are treated by wet powder coating with wettable powder of the present bacterial strain obtained in Seed Treatment In a rotary seed treatment machine ( trade name: HEGE11 , Example 1 in an amount of 1x1010 cfu of the present manufactured by WINTERSTEIGER ) , soybean seeds ( vari bacterial strain per 1 kg of said seeds. ety : “Hatayutaka ” ) are treated by smearing treatment with a A plastic pot is filled with a soil , and then , the seeds thus liquid mixture of the flowable formulation of the present 60 treated are seeded and covered with a soil , which has been bacterial strain and ipconazole prepared in Formulation mixed with damping -off fungus (Rhizoctonia solani ) cul Example 12 (adjusted to 2x1010 cfu of the present bacterial tured in a bran medium . Cultivation is carried out in a strain and 0.2 g of ipconazole per 1 kg of the soybean seeds ). greenhouse under irrigation (“ treated compartment" ) . The A plastic pot is filled with a soil, and then , the seeds thus plants are investigated 20 days after for the number of treated are seeded and covered with a soil, which has been 65 diseased plants , and the disease incidence is calculated by mixed with damping -off fungus ( Rhizoctonia solani) cul the following “ Equation 1 ” . Using untreated seeds, seeding , tured in a bran medium . Cultivation is carried out in a covering with a soil and cultivation are conducted in a US 10,694,750 B2 29 30 similar manner as described above for “ treated compart in Seed Treatment Example 3 ( adjusted to 1x100 cfu of the ment" (" untreated compartment” ) . The plants are investi present bacterial strain per 1 kg of the corn seeds) . In a rotary gated 20 days after for the number of diseased plants , and the seed treatment machine (trade name: HEGE11 , manufac disease incidence is calculated by the following “ Equation tured by WINTERSTEIGER ) , said seeds are treated by 1 ” . Based on the disease incidences of the treated compart- 5 smearing treatment independently with metconazole flow ment and the untreated compartment, the control value of the able formulation (40.0 % flowable formulation , trade name: treated compartment is calculated by the following equation Metolock Fungicide , Valent U.S.A. Corporation , adjusted to “ Equation 2” , and the treated compartment is confirmed to 0.1 g of metconazole per 1 kg of the seeds ), or with have a good control effect on plant disease . tebuconazole flowable formulation ( 28.3 % flowable formu 10 lation , trade name: Raxil 2.6F, Bayer CropScience, adjusted Disease incidence ( % ) = 100x ( number of diseased to 0.1 g of tebuconazole per 1 kg of the seeds) , or with a plant/ total number of seeded seeds ) Equation 1 chemical liquid prepared by dissolving prothioconazole in Control value ( % ) = 100x [ (disease incidence in acetone/ Tween 20 (weight ratio = 95 : 5 ) and diluting with untreated compartment - disease incidence in water (adjusted to 0.2 g of prothioconazole per 1 kg of the treated compartment) / disease incidence in 15 seeds) , or with a chemical liquid prepared by dissolving untreated compartment ] Equation 2 difenoconazole in acetone/ Tween 20 ( weight ratio = 95 : 5 ) The composition of the invention shows a significantly and diluting with water ( adjusted to 0.6 g of difenoconazole higher controlling effect. per 1 kg of the seeds ), or with a chemical liquid prepared by dissolving triticonazole in acetone/ Tween 20 (weight Test Example 7 20 ratio = 95 : 5 ) and diluting with water (adjusted to 0.2 g of triticonazole per 1 kg of the seeds) , or with triadimenol In a rotary seed treatment machine ( trade name: HEGE11 , flowable formulation ( 30 % flowable formulation , trade manufactured by WINTERSTEIGER ), soybean seeds ( vari name: Baytan 30 , Bayer CropScience , adjusted to 1.0 g of ety : “ Hatayutaka” ) are treated by smearing treatment with a triadimenol per 1 kg of the seeds) , or with a chemical liquid chemical liquid prepared by dissolving ipconazole in 25 prepared by dissolving bromconazole in acetone /Tween 20 acetone/ Tween 20 (weight ratio = 95: 5 ) and diluting with (weight ratio = 95 : 5 ) and diluting with water (adjusted to 0.2 water in an amount of 0.2 g of ipconazole per 1 kg of the g of bromconazole per 1 kg of the seeds) . seeds. In a rotary seed treatment machine ( trade name: A plastic pot is filled with a soil , and then , the treated HEGE11 , manufactured by WINTERSTEIGER ), the soy seeds are seeded and covered with a soil , which has been bean seeds thus treated with ipconazole are treated by 30 mixed with damping -off fungus (Rhizoctonia solani) cul smearing treatment with the flowable formulation obtained tured in a bran medium . Cultivation is carried out in a in Seed Treatment Example 3 in an amount of 1x101 ° cfu of greenhouse under irrigation (“ treated compartment” ). The the present bacterial strain per 1 kg of said soybean seeds. plants are investigated 20 days after for the number of A plastic pot is filled with a soil, and then , the seeds thus diseased plants , and the disease incidence is calculated by treated are seeded and covered with a soil , which has been 35 the following “ Equation 1 ” . Using untreated seeds, seeding, mixed with damping -off fungus (Rhizoctonia solani) cul covering with a soil and cultivation are conducted in a tured in a bran medium . Cultivation is carried out in a similar manner as described above for “ treated compart greenhouse under irrigation (“ treated compartment” ) . The ment” ( “ untreated compartment” ). The plants are investi plants are investigated 20 days after for the number of gated 20 days after for the number of diseased plants , and the diseased plants , and the disease incidence is calculated by 40 disease incidence is calculated by the following “ Equation the following “ Equation 1 ” . Using untreated soybean seeds , 1 ” . Based on the disease incidences of the treated compart seeding, covering with a soil and cultivation were conducted ment and the untreated compartment, the control value of the in a similar manner as described above for " treated com treated compartment is calculated by the following equation partment " (" untreated compartment" ) . The plants are inves " Equation 2 ” , and the treated compartment is confirmed to tigated 20 days after for the number of diseased plants , and 45 have a good control effect on plant disease . the disease incidence is calculated by the following “ Equa tion 1 ” . Based on the disease incidences of the treated Disease incidence ( % ) = 100x (number of diseased compartment and the untreated compartment, the control plant/ total number of seeded seeds ) Equation 1 value of the treated compartment is calculated by the fol Control value ( % ) = 100x [ (disease incidence in lowing equation “ Equation 2” , and the treated compartment 50 untreated compartment - disease incidence in treated compartment )/ disease incidence in is confirmed to have a good control effect on plant disease . untreated compartment] Equation 2 Disease incidence ( % ) = 100x (number of ungermi nated seeds/ total number of seeded seeds ) Equation 1 higherThe controllingcomposition effect of the. invention shows a significantly Control value ( % ) = 100x [ (disease incidence in 55 untreated compartment - disease incidence in Test Example 9 treated compartment) /disease incidence in untreated compartment ] Equation 2 In a rotary seed treatmentmachine ( trade name: HEGE11 , The composition of the invention shows a significantly manufactured by WINTERSTEIGER ), soybean seeds (vari higher controlling effect. 60 ety : “ Hatayutaka ” ) are treated by smearing treatment with the flowable formulation of the present bacterial strain Test Example 8 obtained in Seed Treatment Example 3 (adjusted to 1x1010 cfu of the present bacterial strain ) . In a rotary seed treatment In a rotary seed treatment machine ( trade name: HEGE11, machine ( trade name: HEGE11, manufactured by WINTER manufactured by WINTERSTEIGER ), corn seeds ( variety : 65 STEIGER ), the soybean seeds thus treated are treated by yellow dent corn ) are treated by smearing treatment with the smearing treatment with a chemical liquid prepared by flowable formulation of the present bacterial strain obtained dissolving ipconazole in acetone/ Tween 20 (weight US 10,694,750 B2 31 32 ratio = 95 : 5) and diluting with water (adjusted to 0.2 g of The effect on the treated compartment is determined by ipconazole per 1 kg of the seeds ). the following equation , based on the onset area rates of the A plastic pot is filled with a soil, and then , the seeds thus treated compartment and the untreated compartment. treated are seeded and covered with a soil, which has been mixed with damping -off fungus (Rhizoctonia solani) cul- 5 controlling effect= 100x [ (onset area rate of treated tured in a bran medium . Cultivation is carried out in a compartment) / ( onset area rate of untreated com greenhouse under irrigation ( “ treated compartment” ) . The partment) ] Equation : plants are investigated 20 days after for the number of The composition of the invention shows a significantly diseased plants , and the disease incidence is calculated by higher controlling effect. the following “ Equation 1 ” . Using untreated soybean seeds, 10 seededing , covering with a soil and cultivation are con Test Example 11 ducted in a similar manner as described above for " treated compartment ” (“ untreated compartment” ). The plants are In a rotary seed treatmentmachine ( trade name: HEGE11 , investigated 20 days after for the number of diseased plants , manufactured by WINTERSTEIGER ) , corn seeds (variety : and the disease incidence is calculated by the following 15 yellow dent corn ) are treated using a flowable formulation of “ Equation 1” . Based on the disease incidences of the treated the present bacterial strain obtained in Formulation Example compartment and the untreated compartment, the control 18 , metconazole flowable formulation (40.0 % flowable for value of the treated compartment is calculated by the fol mulation , trade name: Metolock Fungicide, Valent U.S.A. lowing equation “ Equation 2 " , and the treated compartment 20 Corporation ), tebuconazole flowable formulation ( 38.7 % flowable formulation , trade name: Folicure 3.6F , Bayer is confirmed to have a good control effect on plant disease . CropScience ), a chemical liquid prepared by dissolving Disease incidence ( % ) = 100x ( number of diseased prothioconazole in acetone / Tween (weight ratio = 95 : 5 ), a plant/ total number of seeded seeds ) Equation 1 chemical liquid prepared by dissolving difenoconazole in 25 acetone/ Tween 20 (weight ratio = 95: 5) , a chemical liquid Control value ( % ) = 100x [ (disease incidence in prepared by dissolving triticonazole in acetone/ Tween 20 untreated compartment - disease incidence in (weight ratio = 95 :5 ), a chemical liquid prepared by dissolv treated compartment) /disease incidence in ing triadimenol in acetone/ Tween 20 (weight ratio = 95: 5 ) , a untreated compartment] Equation 2 chemical liquid prepared by dissolving bromconazole in The composition of the invention shows a significantly 30 acetone/ Tween 20 (weight ratio = 95: 5 ), so that the corn seeds higher controlling effect . retain the present bacterial strain and the compound in the amount shown in Table 1 . Test Example 10 A plastic pot is filled with a soil, and then , the coon seeds, which have been treated with the present bacterial strain , A chemical liquid is prepared by diluting metconazole 35 compound or the present bacterial strain + compound as flowable formulation ( 9 % flowable formulation , trade name: shown in Table 1 , are seeded and covered with a soil , which Sunorg Pro , BASF ) to 400 ppm of metconazole , or by has been mixed with damping -off fungus (Rhizoctonia diluting tebuconazole flowable formulation (20 % flowable solani) cultured in a bran medium . Cultivation is carried out formulation , trade name: ONLY ONE Flowable , Bayer in a greenhouse under irrigation ( “ treated compartment” ) . CropScience ) to 400 ppm of tebuconazole , or by diluting 40 The plants are investigated 20 days after for the number of prothioconazole flowable formulation (41 % flowable for diseased plants , and the disease incidence is calculated by mulation , trade name: PROLINE, Bayer CropScience ) to the following “ Equation 1 ” . Similar procedures are con 400 ppm of prothioconazole, or by dissolving ipconazole in ducted using untreated corn seeds, instead of the treated corn acetone / Tween 20 (weight ratio = 95: 5 ) and diluting with 45 seeds, as described above for the treated compartment water to 400 ppm of ipconazole , or by diluting difenocon ( “ untreated compartment” ) . The plants are investigated 20 azole wettable granules ( 10 % wettable formulation , trade days after for the number of diseased plants , and the disease name: Score wettable granules , Syngenta Japan ) to 400 ppm incidence is calculated by the following “ Equation 1 ” . Based of difenoconazole , or by diluting triticonazole flowable on the disease incidences of the treated compartment and the formulation ( 19.2 % flowable formulation , trade name: Trin- 50 untreated compartment, the control value of the treated ity , BASF ) to 400 ppm of triticonazole , or by diluting compartment is calculated by the following equation “ Equa triadimenol flowable formulation ( 30 % flowable formula tion 2 ” , and the treated compartment is confirmed to have a tion , trade name: Baytan , Bayer CropScience) to 400 ppm of good control effect on plant disease . triadimenol , or by diluting bromconazole flowable formu lation (10 % flowable formulation , trade name: Vectra, Sumi- 55 Disease incidence ( % ) = 100x (number of diseased tomo Chemical Agro Europe ) to 400 ppm of bromconazole , plant/ total number of seeded seeds ) Equation 1 and followed by combining the chemical liquid with the equal volume of a solution of the wettable powder of the Control value ( % ) = 100x [ (disease incidence in present bacterial strain obtained in Seed Treatment Example untreated compartment - disease incidence in 1 ( adjusted to 2x108 cfu of the present bacterial strain ). 60 treated compartment) /disease incidence in The chemical liquid is sprayed in a sufficient amount to untreated compartment] Equation 2 pot planting wheat plants wherein primary leaf development The compartment treated with the composition of the has occurred (variety : “ Shirogane -Komugi ” ). After air dry invention shows a synergistic controlling effect , for each ing, the plants are inoculated with wheat Brown rust fungus combination of the present bacterial strain and the com (Puccinia recondita ) and left to stand under moisture con- 65 pound , compared with that of the corresponding compart dition for 2 days, and the plants are investigated 10 days ment treated solely with the present bacterial strain or the after for the onset area rate . compound . US 10,694,750 B2 33 34 TABLE 1 Test Example 12 Bacteria / Compound retained by Retaining Amount In a rotary seed treatment machine ( trade name: HEGE11 , Seeds ( /Kg seeds ) manufactured by WINTERSTEIGER ) , soybean seeds (vari The present bacterial strain 1 x 1010 cfu 5 ety : “ Hatayutaka ” ) are treated by smearing treatment using The present bacterial strain 1 x 1011 cfu The present bacterial strain 1 x 106 cfu a flowable formulation of the present bacterial strain as The present bacterial strain 1 x 1012 cfu obtained in Formulation Example 18 , and a chemical liquid Metconazole 0.0005 g prepared by dissolving ipconazole in acetone/ Tween 20 Metconazole 0.001 g ( weight ratio = 95 : 5 ) and diluting with water , so that the Tebuconazole 0.01 g 10 soybean seeds retain the present bacterial strain and the Tebuconazole 0.02 g Prothioconazole 0.005 g compound in the amount shown in Table 2 . Prothioconazole 0.01 g A plastic pot is filled with a soil , and then , the soybean Difenoconazole 0.0025 g seeds, which have been treated with present bacterial strain , Difenoconazole 0.005 g compound or the present bacterial strain + compound as Triticonazole 0.005 g Triticonazole 0.01 g 15 shown in Table 2 , are seeded and covered with a soil , which Triadimenol 0.005 g has been mixed with Fusarium root necrosis (Fusarium Triadimenol 0,01 g solani) cultured in a bran medium . Cultivation is carried out Bromconazole 0.005 g in a greenhouse under irrigation ( “ treated compartment" ) . Bromconazole 0.01 g The present bacterial strain 1 x 1010 cfu The plants are investigated 20 days after for the number of Metconazole 0.0005 g 20 diseased plants , and the disease incidence is calculated by The present bacterial strain 1 x 1010 cfu the following “ Equation 1 ” . Similar procedures are con Metconazole 0.001 ducted using untreated soybean seeds, instead of the treated The present bacterial strain 1 x 1010 cfu Tebuconazole 0.01 g soybean seeds, as described above for the treated compart The present bacterial strain 1 x 1010 cfu ment (" untreated compartment” ). Tebuconazole 0.02 g 25 The plants are investigated 20 days after for the number The present bacterial strain 1 x 1010 cfu of diseased plants , and the disease incidence is calculated by Prothioconazole 0.005 g The present bacterial strain 1 x 1010 cfu the following “ Equation 1 ” . Based on the disease incidences Prothioconazole 0.01 g of the treated compartment and the untreated compartment, The present bacterial strain 1 x 1010 cfu the control value of the treated compartment is calculated by Difenoconazole 0.0025 30 the following equation “ Equation 2 ” , and the treated com The present bacterial strain 1 x 1010 cfu Difenoconazole 0.005 g partment is confirmed to have a good control effect on plant The present bacterial strain 1 x 1010 cfu disease . Triticonazole 0.005 g The present bacterial strain 1 x 1010 cfu Disease incidence ( % ) = 100x (number of diseased Triticonazole 0.01 g plant/ total number of seeded seeds ) Equation 1 The present bacterial strain 1 x 1010 cfu 35 Triadimenol 0.005 Control value ( % ) = 100x [ (disease incidence in The present bacterial strain 1 x 1010 cfu untreated compartment - disease incidence in Triadimenol 0.01 g treated compartment) / disease incidence in The present bacterial strain 1 x 1010 cfu untreated compartment] Equation 2 Bromconazole 0.005 g The present bacterial strain 1 x 1010 cfu 40 Bromconazole 0.01 g TABLE 2 The present bacterial strain 1 x 1011 cfu Metconazole 0.0005 g Bacteria / Compound retained Retaining Amount The present bacterial strain 1 x 1011 cfu by Seeds (/ Kg seeds ) Metconazole 0.001 g 45 The present bacterial strain 1 x 1010 cfu The present bacterial strain 1 x 106 cfu The present bacterial strain 1 x 1012 cfu Tebuconazole 0.01 g The present bacterial strain 1 x 106 cfu The present bacterial strain 1 x 1012 cfu Ipconazole 0.005 g Tebuconazole 0.02 Ipconazole 0.01 g The present bacterial strain 1 x 1012 cfu The present bacterial strain 1 x 1010 cfu Prothioconazole 0.005 g 50 Ipconazole 0.005 g The present bacterial strain 1 x 1012 cfu The present bacterial strain 1 x 1010 cfu Prothioconazole 0.01 g Ipconazole 0.01 g The present bacterial strain 1 x 1012 cfu The present bacterial strain 1 x 1012 cfu Difenoconazole 0.0025 g Ipconazole 0.005 g The present bacterial strain 1 x 1012 cfu The present bacterial strain 1 x 106 cfu Difenoconazole 0.005 g 55 Ipconazole 0.01 g The present bacterial strain 1 x 1012 cfu Triticonazole 0.005 g The present bacterial strain 1 x 106 cfu The compartment treated with the composition of the Triticonazole 0.01 g invention shows a synergistic controlling effect , for each The present bacterial strain 1 x 1012 cfu combination of the present bacterial strain and the com Triadimenol 0.005 g 60 pound , compared with that of the corresponding compart The present bacterial strain 1 x 106 cfu ment treated solely with the present bacterial strain or the Triadimenol 0.01 g The present bacterial strain 1 x 1012 cfu compound Bromconazole 0.005 g The present bacterial strain 1 x 106 cfu Test Example 13 65 Bromconazole 0.01 g Spray liquids are prepared and adjusted their concentra tions as shown in Table 3 , respectively , for a flowable US 10,694,750 B2 35 36 powder of the present bacterial strain as obtained in For TABLE 3 - continued mulation Example 13, metconazole flowable formulation ( 9 % flowable formulation , trade name: Sunorg Pro , BASF ) , Bacteria / Compound sprayed Amount of Bacteria / Compound tebuconazole flowable formulation ( 20 % flowable formula to Plants in Spray Liquid (/ L ) tion , trade name: ONLY ONE Flowable , Bayer Crop- 5 The present bacterial strain 1 x 108 cfu Science ) , prothioconazole flowable formulation (41 % flow Ipconazole 0.002 mg able formulation , trade name : PROLINE , Bayer The present bacterial strain 1 x 108 cfu CropScience ), a chemical liquid prepared by dissolving Ipconazole 0.01 mg The present bacterial strain 1 x 108 cfu ipconazole in acetone/ Tween 20 (weight ratio = 95: 5 ) and Difenoconazole 0.001 mg diluting with water , difenoconazole wettable granules ( 10 % 10 The present bacterial strain 1 x 108 cfu wettable formulation , trade name: Score wettable granules, Difenoconazole 0.005 mg Syngenta Japan ) , triticonazole flowable formulation (30.0 % The present bacterial strain 1 x 108 cfu flowable formulation , trade name: ALIOS, BASF ), a chemi Triticonazole 0.002 mg cal liquid prepared by dissolving triadimenol in acetone/ The present bacterial strain 1 x 108 cfu Triticonazole 0.01 mg Tween 20 (weight ratio = 95 : 5) and diluting with water , and 15 The present bacterial strain 1 x 108 cfu bromconazole flowable formulation ( 10 % flowable formu Triadimenol 0.2 mg lation , trade name: Vectra , Sumitomo Chemical Agro The present bacterial strain 1 x 108 cfu Europe ) . Triadimenol mg The liquid ( 50 mL ) is sprayed to pot planting wheat plants The present bacterial strain 1 x 108 cfu wherein primary leaf development has occurred ( variety : 20 Bromconazole 0.2 mg " Shirogane -Komugi " ) . After air drying, the plants are inocu The present bacterial strain 1 x 108 cfu lated with wheat Brown rust fungus and left to stand under Bromconazole mg moisture condition for 10 days (“ treated compartment” ). Also , similar procedures are conducted without spraying the liquid (“ untreated compartment” ) . 25 The effect on the treated compartment is determined by Test Example 14 the following equation , based on the onset area rates of the treated compartment and the untreated compartment . In a rotary seed treatment machine ( trade name: HEGE11 , manufactured by WINTERSTEIGER ) , corn seeds ( variety : controlling effect = 100x [ 1- ( onset area rate of treated compartment )/ (onset area rate of untreated com 30 yellow dent corn ) were treated by smearing treatment using partment ) ] Equation : a flowable formulation of the present bacterial strain as The compartment treated with the composition of the obtained in Formulation Example 19, metconazole flowable invention shows a synergistic controlling effect, for each formulation (40.0 % flowable formulation , trade name: combination of the present bacterial strain and the com Metolock Fungicide , Valent U.S.A. Corporation ), a chemi pound , compared with that of the corresponding compart 35 cal liquid prepared by dissolving tebuconazole in acetone/ ment treated solely with the present bacterial strain or the Tween 20 (weight ratio = 95: 5 ) and diluting with water, a compound . chemical liquid prepared by dissolving prothioconazole in acetone / Tween 20 (weight ratio = 95 :5 ) and diluting with TABLE 3 water, a chemical liquid prepared by dissolving difenocon 40 azole in acetone / Tween 20 (weight ratio = 95: 5 ) and diluting Bacteria / Compound sprayed Amount of Bacteria / Compound with water , a chemical liquid prepared by dissolving triti to Plants in Spray Liquid (/ L ) conazole in acetone/ Tween 20 (weight ratio = 95: 5 ) and dilut The present bacterial strain 1 x 108 cfu ing with water, a chemical liquid prepared by dissolving Metconazole 0.002 mg Metconazole 0.01 mg 45 triadimenol in acetone /Tween 20 (weight ratio = 95 : 5 ) and Tebuconazole 0.1 mg diluting with water , a chemical liquid prepared by dissolving Tebuconazole 0.5 mg bromconazole in acetone/ Tween 20 (weight ratio = 95 : 5 ) and Prothioconazole 0.2 mg Prothioconazole 1 mg diluting with water, so that the soybean seeds retain the Ipconazole 0.002 mg present bacterial strain and the compound in the amount Ipconazole 0.01 mg 50 shown in Table 4 . Difenoconazole 0.001 mg Difenoconazole 0.005 mg A plastic pot was filled with a soil, which has been mixed Triticonazole 0.002 mg with damping -off fungus (Rhizoctonia solani) , and then , the Triticonazole 0.01 mg Triadimenol 0.2 mg corn seeds, which have been treated with present bacterial Triadimenol 1 mg 55 strain , compound or the present bacterial strain + compound Bromconazole 0.2 mg as shown in Table 4 , were seeded . Cultivation was carried Bromconazole 1 g out in a greenhouse under irrigation (“ treated compart The present bacterial strain 1 x 108 cfu Metconazole 0.002 mg ment” ) . The plants were investigated 20 days after for the The present bacterial strain 1 x 108 cfu number of diseased plants , and the disease incidence was Metconazole 0.01 mg calculated by the following “ Equation 1 ” . Also , similar The present bacterial strain 1 x 108 cfu 60 Tebuconazole 0.1 mg procedures were conducted using untreated corn seeds, The present bacterial strain 1 x 108 cfu instead of the treated corn seeds, as described above for the Tebuconazole 0.5 mg treated compartment (“ untreated compartment” ). The plants The present bacterial strain 1 x 108 cfu Prothioconazole 0.2 mg were investigated 20 days after for the number of diseased The present bacterial strain 1 x 108 cfu 65 plants, and the disease incidence was calculated by the Prothioconazole 1 mg following “ Equation 1 ” . The control value of the treated compartment was calculated by the following equation US 10,694,750 B2 37 38 “ Equation 2” , based on the disease incidences of the treated TABLE 4 - continued compartment and the untreated compartment. Retaining Disease incidence ( % ) = 100x ( number of diseased Bacteria /Compound Amount Controlling Estimated plant/ total number of seeded seeds ) Equation 1 5 retained by Seeds (/ Kg seeds) Effect Value * Control value ( % ) = 100x [ (disease incidence in The present bacterial 1 x 1011 cfu 72 39 untreated compartment - disease incidence in strain 0.0005 g treated compartment) / disease incidence in Metconazole untreated compartment ] Equation 2 The present bacterial 1 x 1011 cfu 75 46 strain 0.001 g 10 Metconazole TABLE 4 The present bacterial 1 x 106 cfu 69 31 strain 0.01 g Retaining Tebuconazole Bacteria/ Compound Amount Controlling Estimated The present bacterial 1 x 1012 cfu 78 50 retained by Seeds (/ Kg seeds ) Effect Value * strain 0.02 g 15 Tebuconazole The present bacterial 1 x 1010 cfu 17 The present bacterial 1 x 1012 cfu 89 57 strain strain 0.005 g The present bacterial 1 x 1011 cfu 19 Prothioconazole strain The present bacterial 1 x 1012 cfu 92 63 The present bacterial 1 x 106 cfu 8 strain 0.01 g strain Prothioconazole The present bacterial 1 x 1012 cfu 25 20 The present bacterial 1 x 1012 cfu 89 50 strain strain 0.0025 g Metconazole 0.0005 g 25 Difenoconazole Metconazole 0.001 g 33 The present bacterial 1 x 1012 cfu 92 69 Tebuconazole 0.01 g 25 strain 0.005 g Tebuconazole 0.02 g 33 Difenoconazole Prothioconazole 0.005 g 42 25 The present bacterial 1 x 1012 cfu 56 38 Prothioconazole 0.01 g 50 strain 0.005 g Difenoconazole 0.0025 g 33 Triticonazole Difenoconazole 0.005 g 58 The present bacterial 1 x 106 cfu 61 31 Triticonazole 0.005 g 17 strain 0.01 g Triticonazole 0.01 g 25 Triticonazole Triadimenol 0.005 g 33 30 The present bacterial 1 x 1012 cfu 72 50 Triadimenol 0.01 g 42 strain 0.005 g Bromconazole 0.005 g 17 Triadimenol Bromconazole 0.01 g 33 The present bacterial 1 x 106 cfu 78 47 The present bacterial 1 x 1010 cfu 67 38 strain 0.01 g strain 0.0005 g Triadimenol Metconazole 35 The present bacterial 1 x 1012 cfu 61 38 The present bacterial 1 x 1010 cfu 72 44 strain 0.005 g strain 0.001 g Bromconazole Metconazole The present bacterial 1 x 106 cfu 72 38 The present bacterial 1 x 1010 cfu 61 38 strain 0.01 g strain 0.01 g Bromconazole Tebuconazole The present bacterial 1 x 1010 cfu 72 44 40 * Control value estimated from the calculation by the Colby's equation strain 0.02 g Tebuconazole If the effect by the combination of two active ingredients The present bacterial 1 x 1010 cfu 78 52 is greater than that of the estimated value E , which is strain 0.005 g Prothioconazole calculated by the Colby's equation as follows, the effect is The present bacterial 1 x 1010 cfu 83 59 45 regarded as synergistic . strain 0.01 g Prothioconazole E = X + Y - X - Y / 100 The present bacterial 1 x 1010 cfu 81 44 strain 0.0025 g wherein , Difenoconazole E = Control value when using the mixture of the active The present bacterial 1 x 1010 cfu 89 65 50 ingredients A and B at the concentrations m and n ( amount strain 0.005 g Difenoconazole of the active ingredient ), respectively . The present bacterial 1 x 1010 cfu 56 31 X = Control value when using the active ingredient A at the strain 0.005 g concentration m (amount of the active ingredient) . Triticonazole Y = Control value when using the active ingredient B at the The present bacterial 1 x 1010 cfu 67 38 strain 0.01 g 55 concentration n ( amount of the active ingredient) . Triticonazole The compartment treated with the composition of the The present bacterial 1 x 1010 cfu 67 44 invention showed a synergistic controlling effect , for each strain 0.005 g combination of the present bacterial strain and the com Triadimenol The present bacterial 1 x 1010 cfu 75 52 pound, compared with that of the corresponding compart strain 0.01 g 60 ment treated solely with the present bacterial strain or the Triadimenol compound . The present bacterial 1 x 1010 cfu 61 31 strain 0.005 g Bromconazole Test Example 15 The present bacterial 1 x 1010 cfu 69 44 strain 0.01 65 In a rotary seed treatment machine ( trade name: HEGE11 , Bromconazole manufactured by WINTERSTEIGER ), soybean seeds (vari ety : “ Hatayutaka” ) were treated by smearing treatmentusing US 10,694,750 B2 39 40 the flowable formulation of the present bacterial strain TABLE 5 - continued obtained in Formulation Example 19 and a chemical liquid wherein ipconazole was dissolved in acetone/ Tween 20 Retaining (weight ratio = 95: 5 ) and diluted with water , so that the Bacteria /Compound Amount Controlling Estimated soybean seeds retain the present bacterial strain and the 5 retained by Seeds (/ Kg seeds ) Effect Value * The present bacterial 1 x 106 cfu 77 35 compound in the amount shown in Table 5 . strain 0.01 g A plastic pot was filled with a soil , which has been mixed Ipconazole with damping - off fungus ( Rhizoctonia solani) , and then , the * Control value estimated from the calculation by the Colby's equation soybean seeds, which have been treated with the present 10 bacterial strain , compound or the present bacterial strain + The compartment treated with the composition of the compound as shown in Table 5 , were seeded . Cultivation invention showed a synergistic controlling effect, for each combination of the present bacterial strain and the com was carried out in a greenhouse under irrigation ( “ treated pound , compared with that of the corresponding compart compartment" ) . The plants were investigated 20 days after ment treated solely with the present bacterial strain or the for the number of diseased plants , and the disease incidence 15 compound . was calculated by the following “ Equation 1 ” . Also , similar The invention claimed is : procedures were conducted using untreated soybean seeds, 1. A composition for control plant diseases comprising instead of the treated soybean seeds, as described above for Bacillus strain APM - 1 deposited under ATCC Accession No. the treated compartment (“ untreated compartment” ). The PTA -4838 and one or more ergosterol biosynthesis inhibitor, plants were investigated 20 days after for the number of 20 wherein the composition comprises the ergosterol biosyn diseased plants , and the disease incidence was calculated by thesis inhibitor in an amount of 10-5 to 102 g per 1010 cfu of Bacillus strain APM - 1 . the following “ Equation 1 ” . The control value of the treated 2. The composition according to claim 1 wherein the compartment was calculated by the following equation ergosterol biosynthesis inhibitor is selected from the group “ Equation 2” , based on the disease incidences of the treated 25 consisting of tetraconazole , metconazole , tebuconazole , pro compartment and the untreated compartment. thioconazole , ipconazole, difenoconazole , triticonazole , propiconazole , triadimenol, prochloraz , fluquinconazole , Disease incidence ( % ) = 100x (number of diseased hexaconazole , epoxiconazole , flutriafol, triadimefon , plant/ total number of seeded seeds ) Equation 1 pefurazoate and bromuconazole . 30 3. The composition according to claim 1 wherein the Control value ( % ) = 100x [ (disease incidence in ergosterol biosynthesis inhibitor is selected from the group untreated compartment - disease incidence in consisting of metconazole , tebuconazole , prothioconazole , treated compartment ) /disease incidence in ipconazole , difenoconazole , triticonazole , triadimenol, and untreated compartment ] Equation 2 bromuconazole . 4. A plant seed or a vegetative propagation organ com 35 prising 10% to 1013 cfu of Bacillus strain APM - 1 deposited TABLE 5 under ATCC Accession No. PTA -4838 and 0.0001 to 10 g of Retaining one or more ergosterol biosynthesis inhibitor, per 1 kg of the Bacteria /Compound Amount Controlling Estimated seed or vegetative propagation organ . Effect Value * 5. The plant seed or a vegetative propagation organ retained by Seeds (/ Kg seeds ) 40 according to claim 4 wherein the ergosterol biosynthesis The present bacterial 1 x 1010 cfu 10 strain inhibitor is selected from the group consisting of tetracon The present bacterial 1 x 1012 cfu 13 azole , metconazole , tebuconazole , prothioconazole , ipcon strain azole , difenoconazole , triticonazole , propiconazole , triadi The present bacterial 1 x 106 cfu 3 menol, prochloraz , fluquinconazole , hexaconazole , strain 45 epoxiconazole , flutriafol, triadimefon , pefurazoate and bro Ipconazole 0.005 g 27 muconazole . Ipconazole 0.01 g 33 6. A method for controlling plant diseases, comprising a The present bacterial 1 x 1010 cfu 63 34 step of applying Bacillus strain APM -1 deposited under strain 0.005 g ATCC Accession No. PTA - 4838 and one or more ergosterol Ipconazole biosynthesis inhibitor to a plant or a plant cultivation site , The present bacterial 1 x 1010 cfu 73 39 50 strain 0.01 g wherein the ergosterol biosynthesis inhibitor is applied in Ipconazole an amount of 10-5 to 102 g per 1010 cfu of Bacillus The present bacterial 1 x 1012 cfu 70 36 strain APM - 1 . strain 0.005 g 7. The method for controlling plant diseases according to Ipconazole claim 6 wherein the plant is a genetically modified plant.