Current Approaches in Life Sciences for Sustainable Development” (ICCALSSD-2021)”

Rayat Shikshan Sanstha’s YASHAVANTRAO CHAVAN INSTITUTE OF SCIENCE, SATARA (Autonomous) Reaccredited by NAAC (3rd cycle) with A+ Grade (CGPA 3.57), College with potential for Excellence (UGC), FIST by DST, Star scheme by DBT, Excellence by RUSA and ISO 9001:2015 Certified

International E-Conference on “Current Approaches in Life Sciences for Sustainable Development” (ICCALSSD-2021)”

Jointly Organized by, ABSTRACTS Department of Botany Department of Zoology

Department of Microbiology

Department of Biotechnology

Department of Food Processing and Packaging Yashavantrao Chavan Institute of Science, Satara (MS), India 415002 Rashtriya Uchchtar Shiksha Abhiyan (RUSA) Sponsored

International E-Conference on “Current Approaches in Life Sciences for Sustainable Development” (ICCALSSD-2021) 19th – 20th March 2021

ABSTRACTS

Jointly Organized by Department of Botany, Department of Zoology, Department of Microbiology, Department of Biotechnology and Department of Food Processing and Packaging, Yashavantrao Chavan Institute of Science (Autonomous College), Satara – 415 001, Maharashtra, INDIA Disclaimer:

 The authors are responsible for the contents of abstract/synopsis.

 The publishers or editors do not take any responsibility for the same in any manner.

 Abstracts are printed as per content provided by the authors.

Published by: Dr. B. T. Jadhav, Principal, Yashavantrao Chavan Institute of Science, Satara – 415 001. Maharashtra, INDIA Our Inspiration

Karmaveer Bhaurao Patil International E-Conference on “Current Approaches in Life Sciences for Sustainable Development” (ICCALSSD-2021) March 19 & 20, 2021

Chief Patron

Hon. Padmavibhushan Sharadchandraji Pawar President, Rayat Shikshan Sanstha, Satara

Patrons

Hon. Dr. Anil Patil Hon. Dr. N. D. Patil Chairman, Rayat Shikshan Sanstha, Ex- Chairman, Rayat Shikshan Sanstha, Satara Satara

Prin. Dr. V. S. Shivankar Prin. Dr. Mrs. P. S. Gaikwad Secretary, Rayat Shikshan Sanstha, Joint Secretary, Rayat Shikshan Sanstha, Satara Satara Prin. Dr. S. G. Menkudale Auditor, Rayat Shikshan Sanstha, Satara

International Advisory Committee

Dr. Claudete Santa-Catarina Dr. Johannes Van Staden Professor, Global Universidade Estadual Director, Research Centre for do Norte Fluminense, Brazil Plant Growth and Development, Dr. Shrihari Arjunwadkar University of KwaZulu-Natal, Consultant, Regulatory Affairs, Canada. South Africa Padmashree Prof. Pramod Tandon Prof. Azamal Husen CEO, Biotech Park, Lucknow & Former Vice-Chancellor Wolaita Sodo University, P.O. Box. 138, of North-Eastern Hill University, Shillong. Wolaita, Ethiopia. Dr. Gajanan Ghodake Dongguk University, Seoul, Korea.

National Advisory Committee

Hon. Dr. D. T. Shirke Hon. Dr. P. S. Patil Vice Chancellor, Shivaji University, Pro Vice Chancellor, Shivaji University, Kolhapur Kolhapur Dr. Vijay Joshi Dr. P. N. Pabrekar Former I/C Jt. Director, RUSA, Mumbai. Consultant, RUSA, Mumbai

International E-Conference on “Current Approaches in Life Sciences for Sustainable Development” (ICCALSSD-2021) March 19 & 20, 2021

Dr. V. S. Mane Dr. Mrs. V. D. Jadhav Professor & Head, Department of Zoology, Professor and Head, Dept. of Botany, Shivaji University, Kolhapur Shivaji University, Kolhapur Dr. Mrs. J. P. Jadhav Dr. K. D. Sonavane Professor and Head, Department of Biochemistry, Professor and Head, Dept. of Microbiology, Shivaji University, Kolhapur Shivaji University, Kolhapur Dr. Yogesh Shouche Scientist, National Center for Cell Science (NCCS), Pune

Organizing Committee

President – ICCALSSD-2021 Vice President – ICCALSSD-2021 Dr. B. T. Jadhav Dr. V. Y. Deshpande Principal, Yashavantrao Chavan Institute of Science, Vice Principal, Yashavantrao Chavan Satara Institute of Science, Satara

Chairman - ICCALSSD-2021 Vice Chairman - ICCALSSD-2021 Mr. S. S. Gaikwad Mrs. N. S. Joshi Head, Department of Botany, Head, Department of Microbiology, Yashavantrao Chavan Institute of Science, Yashavantrao Chavan Institute of Satara Science, Satara

Convener - ICCALSSD-2021 Co-convener - ICCALSSD-2021 Dr. R. B. More Dr. Mrs. S. S. Wadikar Dr. J. J. Chavan Prof. (Dr.) S. A. Bhoite

Coordinator - ICCALSSD-2021 Co-coordinator - ICCALSSD-2021 Dr. V. M. Ghorpade Dr. M. L. Ahire Dr. Mrs. S. P. Nalawade Dr. Ms. P. S. Patil

Organizing secretaries - ICCALSSD-2021 Treasurers - ICCALSSD-2021 Mrs. R. A. Shinde Dr. G. S. Jadhav Dr. P. S. Mundada Mr. P. R. Dure

Organizing Committee Members

Dr. D. R. Hasabe Dr. A. P. Torane Dean, PG Studies and Head, Dean, UG, Studies Department of Mathematics Dr. S. H. Mujawar Dr. S. R. Bhongale Dean, Research and Development, Dean, Diploma Courses Coordinator, Dept. of Nanosci. & Tech.

International E-Conference on “Current Approaches in Life Sciences for Sustainable Development” (ICCALSSD-2021) March 19 & 20, 2021

Dr. P. K. Pagare Mr. V. R. Dhaigude Academic Registrar Head, Department of Physics

Dr. H. P. Umap Mr. J. A. Wagh Controller of Examination and Head, Head, Department of Electronics, Department of Statistics Computer Science and B. Voc. Mr. P. V. Bhise Dr. Mrs. M. A. Patil Head, Department of Chemistry Head, Department of English Dr. Ms. A. R. Mali Dr. A. D. Khobragade Coordinator, Dept. of Forensic Science Librarian Mr. D. S. Gaikwad Director of Physical Education

All Teaching and Non-teaching staff of Yashavantrao Chavan Institute of Science, Satara (Autonomous College)

Working committees

Welcome committee Dr. M. L. Ahire Prin. Dr. B. T. Jadhav - Chairman Dr. G. S. Jadhav Prof. (Dr.) V. Y. Deshpande Dr. P. S. Mundada Mr. S. S. Gaikwad Report committee Mrs. N. S. Joshi Dr. M. L. Ahire – Chairman Dr. Mrs. S. S. Wadikar Dr. Ms. P. S. Patil Prof. (Dr.) S. A. Bhoite Dr. Mrs. A. J. Salunkhe Dr. R. B. More Dr. J. J. Chavan Virtual Engagement & website All Dean’s and HoD’s Committee Dr. Mrs. S. S. Kanase – Chairman Programme Committee Dr. D. M. Karanjkar Dr. R. B. More - Chairman Dr. Ms. S. M. Deshpande Dr. J. J. Chavan Dr. S. G. Jadhav Dr. Mrs. S. P. Nalawade Mr. Satish Doke Mrs. Rohini Shinde Mr. Kulkarni (OIT)

Editorial Board for Proceeding Announcement & Anchoring & E-Abstract Book Mrs. R. A. Shinde – Chairman Dr. V. M. Ghorpade – Chairman Ms. Medha Bodhe Dr. J. J. Chavan Mr. Abhinav Mali Dr. Mrs. S. P. Nalawade

International E-Conference on “Current Approaches in Life Sciences for Sustainable Development” (ICCALSSD-2021) March 19 & 20, 2021

Resource person Mr. Kailas Kumbhar communication & vouchers Dr. G. S. Jadhav - Chairman Registration, Feedback Mr. J. R. Yadav Committee Mr. P. R. Dure Mr. H. L. Shinde- Chairman Mr. D. N. Nimbalkar Mr. S. J. Koli Mr. Bandu Pawar Ms. D. D. Dhumal Ms. M. M. Raut Publicity & Photography Dr. Mrs. S. P. Nalawade - Certificate committee Chairman Mrs. G. V. Utekar - Chairperson Dr. N. B. Mane Dr. Ms. S. P. Zanke Mrs. V. S. Patil Ms. Madhuri Jadhav Dr. Ms. Archana Salunkhe

Dr. Anil Patil Chairman Rayat Shikshan Sanstha, Satara.

Date: 18/03/2021

MESSAGE It is indeed a pleasure to know that Yashavantrao Chavan Institute of Science, Satara has taken lead to organize International E-conference on “Current Approaches in Life Sciences for Sustainable Development” (ICCALSSD-2021)” on 19th – 20th March 2021 and bring out a souvenir to mark the occasion.

This century is known to be the century of Biology (Life Sciences) and presently, all the leading countries of the world are dedicating significant attention towards advanced research in life sciences for sustainable development. It is also a very promising field for young scholars and researchers as it offers great employment and research opportunities in future. The conference will bring the scientists, academicians, technicians and researchers together to exchange their views and ideas for the better understanding of current approaches in life sciences for sustainable development. I am hopeful that this forum will pave the way for new ideas instrumental in making the world a better place for the future generations.

I congratulate the Principal, Faculty members, staff and students of the college for organizing this international e- conference and offer my best wishes to all the participants and scientists. Telegram: RAYAT SATARA Fax No.: 231424 STD No.: 02162 234566, 233857, 232444 “Education through self-help is our motto”, KARMAVEER RAYAT SHIKSHAN SANSTHA, SATARA. Maharashtra State (INDIA) Founder: Karmaveer Bhaurao Patil, D.Litt. Estd.: 1919 Website: www.rayatshikshan .edu Dr. V. S. Shivankar Date: 18/03/2021 E-mail: [email protected] Secretary

MESSAGE It is my great honor and pleasure to welcome you all to the International E-conference on “Current Approaches in Life Sciences for Sustainable Development” (ICCALSSD-2021)” on 19th – 20th March 2021 organized jointly by Department of Botany, Zoology, Microbiology, Biotechnology and Food Processing and Packaging of the Yashavantrao Chavan Institute of Science, Satara. The theme of the conference is important in the current context of sustainable development of natural resources. This conference will provide a prestigious international e-platform in the COVID-19 pandemic situation for bringing together students, researchers and academicians to exchange experience and expertise. I sincerely hope that you will enjoy the conference key note address, plenary and invited talks, oral and poster presentations. I welcome all the participants, resource persons and delegates at this event and extend my best wishes for this e-conference. Telegram: RAYAT SATARA Fax No.: 231424 STD No.: 02162 234566, 233857, 232444 “Education through self-help is our motto”, KARMAVEER RAYAT SHIKSHAN SANSTHA, SATARA. Maharashtra State (INDIA) Founder: Karmaveer Bhaurao Patil, D.Litt. Estd.: 1919 Dr. Mrs. P. S. Gaikwad Website: www.rayatshikshan .edu Date: 18/03/2021 Joint Secretary E-mail: [email protected]

MESSAGE

Department of Botany, Zoology, Microbiology, Biotechnology and Food Processing and Packaging of the Yashavantrao Chavan Institute of Science, Satara are organizing an International E-conference on “Current Approaches in Life Sciences for Sustainable Development” (ICCALSSD-2021)” on 19th – 20th March 2021.

I am confident that this multidisciplinary approache in life sciences will cover all aspects of life sciences including biodiversity and its conservation, natural resource management and their sustainable utilization. The deliberations will enhance the applicability of the knowledge for the betterment of mankind and mother earth.

I welcome all the participants, resource persons and delegates at this event and extend my best wishes for this e-conference. Prin. Dr. B. T. Jadhav Director, Yashavantrao Chavan Institute of Science, Satara

From Principal’s Desk Date: 18/03/2021

I am indeed very happy to offer a hearty welcome to all the delegates and other guests on the occasion of International E- conference on “Current Approaches in Life Sciences for Sustainable Development” (ICCALSSD-2021)” on 19th – 20th March 2021 jointly organized by Department of Botany, Zoology, Microbiology, Biotechnology and Food Processing and Packaging of Yashavantrao Chavan Institute of Science, Satara. For us it is a great privilege and honor to have the opportunity for organizing this International E- Conference.

We have organized, so far, a large number of international, national and state level conferences/seminars and workshops in our campus. We always take initiatives in organizing such conferences by realizing the importance of exchange of intellectual and scholarly thoughts and ideas that will exchange at such academic events. But due to COVID-19 pandemic situation it is not possible to organize the on-campus conference, therefore, we decided to organize E-conference so that everyone can enjoy the academic feast at their convenience.

I am happy to note that the theme of this conference is the “Current Approaches in Life Sciences for Sustainable Development” (ICCALSSD-2021)” which will provide a great opportunity for all the students, researchers and academicians in life sciences to interact at interdisciplinary level. I welcome you all at this event and extend my best wishes for this conference. Dr. R. B. More Dr. J. J. Chavan Convener ICCALSSD-2021

Date: 18/03/2021 From Organizer’s Desk

It’s a great moment of pleasure for us to welcome you all for an auspicious occasion of International E-conference on Current Approaches in Life Sciences for Sustainable Development” (ICCALSSD-2021)” on 19th – 20th March 2021 jointly organized by Department of Botany, Zoology, Microbiology, Biotechnology and Food Processing and Packaging of Yashavantrao Chavan Institute of Science, Satara. The conference is patronized by Hon. Padmavibhushan Sharadchandraji Pawar, President; Hon. Dr. Anil Patil, Chairman; Hon. Dr. N. D. Patil, Ex-Chairman; Hon. Prin. Dr. V. S. Shivankar, Secretary and Hon. Prin. Dr. Mrs. P. S. Gaikwad, Joint Secretary of Rayat Shikshan Sanstha, Satara.

I am very glad to inform you that Prof. (Dr.) M. M. Salunkhe, Vice Chancellor, Bharati Vidyapeeth, Pune is inaugurating the conference and Prin. (Dr). V. S. Shivankar, Secretary, Rayat Shikshan Sanstha, Satara is presiding over the occasion, in gracious presence of Prin. Dr. Mrs. P. S. Gaikwad and Prin. Dr. B. T. Jadhav.

Dr. Sudeshna Chandra, Professor and Humboldt Fellow, Dept. of Chemistry, NMIMS University, Mumbai is to start the proceedings with key note address. Dr. Prabodh Halade, President AFST, Mumbai; Head, Technical Regulatory Marico Ltd., MumbaiDr. Hatem Ahmed M. Shabana, Researcher, Sharjah Research Academy, Sharjah, UAE.; Dr. Sudhir K. Sinha, CEO, InnoGenomics Technologies, New Orleans, LA, USA; Dr. Shrihari Arjunwadkar, Consultant, Regulatory Affairs, Canada, Winnipeg and Dr. Amar Telke, Senior Scientist, Norwegian Veterinary Institute, Oslo are presenting their research as invited speakers. With the advent of recent advances in life sciences such as molecular biology, genetic engineering and emergence of biotechnology, the teaching and research in biological sciences expanded its horizons to various fields of interdisciplinary interests.

Hence it is planned to bring together all researchers in the field of biological research across the nation and to the globe to deliberate upon the future focus of teaching research and technology development in life sciences in our country. The thrust area of the conference opens a venue for the discussion on highly important and relevant topics such as Biochemistry; Biodiversity & Biotechnology; Biodegradation and Phytoremediation; Biofuel; Biotechnology for agriculture and human health; Climate Change; Nutrition & diet during Covid-19 crises; Environmental Microbiology; Fishery Science; Forensic Biology; Food processing; Marine Biology; Molecular biology; Nanotechnology; Organic Farming; Physiology improvement against environmental stress; Plant biotechnology; Plant- microbe Interactions; Postharvest food processing; Sustainable Agriculture; Toxicology etc. the discussions and interactions during conference certainly will be incentive to bring positive change in the field of knowledge of life science. We are also assured that conference will be productive and it will provide a platform to generate research culture in the field of life science.

We once again hearty welcomes to all the participants and wish the conference a grand success.

International E-Conference on “Current Approaches in Life Sciences for Sustainable March 19 & 20, Development” (ICCALSSD-2021) 2021

Page Sr No Author Title of Research Paper No. INVITED TALKS Priyal Chikhaliwala and Development of nano-biosensor for 1. i Sudeshna Chandra early detection of liver cancer Agricultural & Rural Distress In 2. Shrihari J. Arjunwadkar INDIA, Global Warming - iii Microbiological Facets Applications of NGS in surveillance of 3. Amar Anandrao Telke v human and animal pathogens ORAL PRESENTATIONS Sandip S. Kale, Suresh Phytochemical screening and in-vitro OR 01 S. Kale, Padma B. biological activities of leaf extract of 2 Dandge Argyreia cuneata Lour. Ascertaining bacteriological pollution OR-02 Ivan Aranha 3 in bottled drinking water Drought tolerance in Sorghum bicolor OR-03 Ravindra N. Deshmukh 4 under water stress Validated HPTLC method for Manoj M. Khavate, estimation of metabolite Embelin OR- 04 5 Suresh D. Jagtap from traditional medicinal plants from Myrsinaceae family Antidiabetic effect of ethanolic leaves T. A. Gangavane, R.D. OR- 05 extract of Aegle marmelos on alloxan 6 Bodare, R. B. More induced diabetic aged mice Screening of rhizospheric microorganisms for developing OR- 06 Deepak U. Bhusare 7 effective plant growth promoting bio- inoculant Effect of different media on In vitro Vidya V. Kamble*, OR- 07 seed germination of Embelia ribes 8 Nikhil B. Gaikwad Burm F.: a medicinally important liana Kirdat P. N., Dandge P. Extraction of acid soluble collagen OR- 08 9 B. from marine fish waste Cataloguing bacterial community in Vineet Vishal, the rhizosphere of Shorea robusta OR-09 Geetanjali Singh, 10 Gaertn using portable nanopore Shalini Lal sequencer-MinION

International E-Conference on “Current Approaches in Life Sciences for Sustainable March 19 & 20, Development” (ICCALSSD-2021) 2021

Comparative study of solubility of Aditya More, Smitesh OR-10 tranquilizers (alternative Date Rape 11 Nalage, Anita Mali Drugs) in Stomach Acid Evaluation of growth and protein Janardhan Namdeo OR-11 content in Scytonema schmidtii Gom. 13 Nehul in different nutrient media Mitochondrial gene (mtCOI Gawai T. B., Gawande S. cytochrome oxidase I) diversity of OR-12 14 J., Ade A. B., Singh M. thrips tabaci infesting onion (Allium cepa L.) in India Ecological notes on epiphytic fern of OR-13 S. D. Shaikh northern western ghats of 15 Maharashtra Vishal V. Naik1*, Amol Salinity tolerance of Portulaca V. Patil2, Vishal T. OR-14 oleracea L. is facilitated by enhanced 16 Aparadh3, Baburao A. oxalic acid synthesis Karadge4 Study on removal of viruses using OR-15 V V Sawant, V S Sawant 17 pulsed electrical field Suhas Kundlik Kamble, Influence of plant extract formulation OR-16 Yuvraj Dhondiram on photosynthetic pigments of bean 19 Kengar during rust disease management Vinaya Sathe, Prakash A novel approach of synthesis ZnO Loni, Prajakta Kamble, nanoparticles from starch: Its OR-17 20 Shradha Khamitkar, characterization and its efficient Suresh Suryawanshi biomedical applications Desai S. S., Bhoyar P. Effect of temperature and composition OR-18 21 R., Kadam S. L. of fabrics on fabrics stained with blood Sushama S. Desai, Significance of Diatoms in drowning OR-19 22 Piyusha P. Rajguru deaths AMF associated with bt and non BT V. V. Kamble, R. B. Yele, OR-20 cotton crop from Man tehsil of Satara 23 D. V. Rajmane district (M.S.) India OR-21 Sachin V. Tawade Incidence of leaf blight on Safed Musli 24 Study on Antibiotic resistance profile of coliforms isolated from drinking OR-22 Omprakash A. Kadam 25 water resources of Hadgaon Region

International E-Conference on “Current Approaches in Life Sciences for Sustainable March 19 & 20, Development” (ICCALSSD-2021) 2021

Chelate assisted phyto-accumulation Rohit A. Kale, Vinayak of Chromium and its effect on growth, OR-23 H. Lokhande, Avinash physiological and biochemical 27 B. Ade responses in the weed, Portulaca oleracea L. in hydroponic conditions P. S. Mundada, H. A. Visualizing the distribution of silicon Nikule, S. D. Umdale, S. in different plant parts of finger millet OR-24 Anil Kumar, V. T. (Eleusine coracana (L.) Gaertn.) using 29 Barvkar, T. D. Nikam1, electron dispersive spectroscopy M. L. Ahire (EDS) V. Y. Deshpande, M. D. Cypermethrin persuaded instabilities OR-25 30 Sawant, P. S. Pawar of digestive enzymes in Labeo rohita In-vitro rooting and hardening of OR-26 S. V. Madhale excised shoots of Momordica 31 cymbalaria Hook. f. In vitro multiplication and Mukta Rajaram assessment of genetic stability of Bhamare, OR-27 Ceropegia lawii Hook. An endemic 32 Hemantkumar and critically endangered medicinal Atmaram Thakur plant of Western Ghats Neurotoxic effect of DDVP on Shelar A. S., economically important freshwater OR-28 Suryawanshi P. S., 33 fish Pungasius bocourti (Sauvage, Pawar P. S. 1880) POSTER PRESENTATIONS Effect of occupational environment on Irumjahan Khan, R. B. PO-01 respiratory health of female workers in 35 More brick industry Isolation of Pseudomonas sp. from rhizospheric soil and polluted water for A. A. Gangawane, N. T. PO-02 determination of the PGPR (plant growth 36 Pawar, A. U. Sutar promoting rhizobacteria) activity along with its applications Mayur S. Deshmukh1*, Integrated nutrient management and PO-03 37 Dipti D. Ghadge2 maintaining soil fertility Study of morphology with respect to D. P. Patil*, V. B. shape and size of pollen grain PO-04 38 Chopade (micrometry) from family Malvaceae and Apocynaceae

International E-Conference on “Current Approaches in Life Sciences for Sustainable March 19 & 20, Development” (ICCALSSD-2021) 2021

Studies on judicious doses of nitrogen fertilizer and biofertilizer on yield PO-05 Bendre K. B. 39 performance of fodder crop maize (cv. Ganga Safed - 2) Study of phytoplankton and A. R. Bhosale, S. S. PO-06 hydrobiology in water bodies of Satara 40 Gaikwad city Effects of pesticides on phyto S. B. Ghorpade, S. A. PO- 07 constituents of vegetables and fruits 41 Shinde-Inamdar in Sangli and Satara District Bagal R. B., Bodake V. A., Bendre N. N., Pawar Analysis of microbial diversity from PO- 08 P. A., Wadikar S. S., soil samples of various habitats 42 Khedakar G. D., Bhoite through DNA sequencing S. H. N. B. Salunkhe, S. M. Floral diversity analysis of Kushi PO- 09 43 Deshapande village and adjoining areas Zooplankton diversity of Uttarmand Gaikwad V. D., More R. PO- 10 reservoir in Patan Tahsil, District 44 B., Padule A. R. Satara (M. S.) India Assessment of arbuscular mycorrhizal P. S. Jadhav, N. B. PO-11 fungi and soil characterization of 45 Mane Caralluma fimbriata Wall. Arbuscular Mycorrhizal Fungi associated with variety of Crotolaria PO- 12 S. T. Wagh, N. B. Mane 46 pallida Aiton. found in drought prone area of Shendre area in Satara District Allelopathic effects of two common Shivani S. Gaikwad, weeds on germination and seedling PO- 13 47 Abhinav A. Mali* growth of two seasonal crops in Satara district Effect of pomegranate juice and peel C. B. Jamdade, R. D. extract on dimethoate toxicity studied PO- 14 Bodare, V. Y. 48 on protein profile in liver and kidney Deshpande, R. B. More of aged mice Synergistic effect of agrochemicals (herbicides) on development of PO-15 V. B. Chopade 49 streptocycline resistance in Xanthomonas axonopodis

International E-Conference on “Current Approaches in Life Sciences for Sustainable March 19 & 20, Development” (ICCALSSD-2021) 2021

Nitin Gore, Kajal Patil, Sandhyarani Khilare, Sumaiya Shaikh, Proximate composition and Hanmant Shinde, phytochemical analysis of different PO-16 50 Abhinav Mali, Pankaj plant parts of Vigna stipulacea Mundada, Suraj Kuntze Umdale, Mahendra Ahire Comparative account of micro- Sonal D. Shinde, morphological features among Swapnaja M. different accessions of Jasminum PO- 17 Deshpande, Sunil H. 51 multiflorum (Burm.f.) Andr. Bhoite, (Oleaceae) from Satara, Maharashtra Jaykumar J. Chavan (India) A. A. Survase, Shivangi TiO2 nanoparticles synthesis by an PO-18 52 Shivraj Kanase isolated fungus Zinc induced alternations in protein, Karanjkar D. M., V. Y. glycogen and lipid contents in tissues PO- 19 53 Deshpande of freshwater fish Gonoproktopterus kolus (Sykes) Dipti D. Ghadge, Mayur Comparison of sustainable methods of PO- 20 S. Deshmukh, Smitesh water purification: seed extract and 54 Nalage, S. T. Mahanwar charcoal Abhishek R. Ranvare, Harshad R. Nikam, Development of multigrain sweet PO- 21 55 Abhishek P. Pujari, potato pocket samosa Pandurang Dure Aeluropus lagopoides and Suaeda Pawar Uday S., Joshi PO-22 nudiflora potential halophyte species 56 Arvind J. for phytoremediation Shreedhar S. Savekar*, Phytochemical analysis from rhizome PO- 23 57 Yogesh R. Chavan of Zingiber zerumbet Smith Monali Khatal*, Aboli Sustainable methods for synthesis of PO-24 Sapkal, Suraj Aattar, 58 indole derivatives Santosh Kamble S. P. Desai1*, A. G. Collection, conservation of non- Jadhav1, Y. A. Phonde2, PO-25 basmati aromatic rice landraces and 59 S. G. Ghane1, N. B. their use for sustainable agriculture Gaikwad1

International E-Conference on “Current Approaches in Life Sciences for Sustainable March 19 & 20, Development” (ICCALSSD-2021) 2021

The study of pre-treatments effect on Misal Saraswati Dattu, PO- 26 fishy flavor and nutritional value of 61 Jadhav M. H. fish powder Ranvare Abhishek Rajendra, Harshad R. Development and sensory evolution of PO- 27 62 Nikam, Abhishek S. Oats and puffed rice chocolate Pujari, Pandurang Dure Study of diseases and pests of Ginger PO- 28 P. S. Rasal, B. A. Kore 63 in Satara district Proposing application of 3D scanning Anantkumar R. Patil, technology and machine learning in PO- 29 Samidha S. Walvekar, 64 crime scene documentation and Manjushri S. Bagul autopsy Sneha Chatterjee, Green synthesis of Copper PO- 30 Vaibhavi Gulwani, nanoparticles from plant extracts & 65 Pratibha Patil its antimicrobial activity Avadhut Patil, Pradhumn Shitole, Microbial production of melanin from PO- 31 66 Rishikesh Rajput, farm yard compost Pratibha Patil Bhosale P. C, Wadikar Isolation of antibiotic producing PO- 32 67 S. S Endophytes in cactus Opuntia humifusa Collection, conservation, beta carotene A. G. Jadhav, S. P. and mineral analysis studies of PO- 33 Desai, S. G. Ghane, Y. A. 68 nonaromatic rice landraces from Phonde, N. B. Gaikwad Kolhapur district of Maharashtra Avdhut D. Kadam, Ankita Phadatare, Use of heterogeneous catalyst in PO- 34 69 Rutuja Pawar, Santosh organic transformation B. Kamble Patil V. S., Bhosale S. Biodegradation of oil sludge waste by PO- 35 L., Shete S. S., Sawant 70 using fungi P. S., Patil P. S. Pradnya B. Zambare, Biosynthesis of Copper nanoparticles PO- 36 71 Shivangi Shivraj Kanase by an isolated fungus Avdhoot Patil, Akshay Antibacterial properties of elicitor Deshmukh, Pallavi PO-37 treated callus cultures of Salacia 72 Panari, Sarika Chavan, chinensis L. Jaykumar Chavan

International E-Conference on “Current Approaches in Life Sciences for Sustainable March 19 & 20, Development” (ICCALSSD-2021) 2021

Mayuri Mohan Pawar, PO- 38 lnstant multigrain chicken curry mix 73 Nilam Sanjay Bhosale Rutuja Zanjurne, Antimicrobial activity of plant leaf Devyani Pawar, PO- 39 extracts against plant pathogenic 74 Aishwarya Khutale, microorganisms Pratibha Patil S. H. Nadaf , P. B. Isolation and partial purification of L- PO- 40 75 Dandage, N. H. Nadaf glutaminase from halophilic bacteria Akshata S. Phalke, A Microbial Approach: A sustainable PO- 41 76 Pramod V. Chavan way for plastic degradation Preparation of herbal formulation from leaves of Oscimum americanum Tuwar D. A., Satre T. S., PO- 42 & Eclipta alba and it's efficiency 77 Tuwar A. R. against the fungal diseases and wounds in dairy animals Anjali T. Gujar*, Effects of pharmaceutical (Diclofenac) PO- 43 Shradha A. Kshirsagar, 78 in fresh water fish Garra mullya V. Y. Deshpande Formulation and sensory evolution of PO- 44 Kiran Hiralal Pawar ready-to-cook multigrain chicken 79 meat balls Shinde Shraddha, Study of physicochemical and Kulkarni Asmita, PO- 45 microbial analysis of soil from Kaas 80 Salunkhe Mayur, plateau Kumbhar Kailas Effects of dietary inclusion of Nalavade Virdhaval synbiotics on protein content, enzyme PO-46 Madhav, Nalawade 81 activity and economic parameters in Savita Pravin Bombyx mori L. Antioxidant, cytotoxicity and Sharad G. Jadhav, quantum chemical descriptor study of PO- 47 Vishakha Deshmukh, 82 several coumarin derivatives: Aishwarya Patil computational analysis Applications of melanin associated Swati T. Gurme, Suchita silver nanoparticles for photocatalysis PO- 48 83 V Jadhav of malachite green dye and it’s phytotoxicity analysis U. L. Shevale1,2*, Jadhav Microbial and Biochemical studies on PO- 49 84 P. P.1, S. M. Pawar2 gut bacteria isolated from fresh water

International E-Conference on “Current Approaches in Life Sciences for Sustainable March 19 & 20, Development” (ICCALSSD-2021) 2021

fish Garra mullya Amruta J. Ranaware*, Rasika R. Mane, Pavani PO- 50 Development of instant fish soup mix 85 V. Gadpurpwar, Shital A. Patil, A. K. Sahoo Sanchita Pandit, Balaji Extraction and characterization of PO- 51 86 Kanwate collagen from scales of Catla catla Production and characterization of Yasmin A. Mulani, PO- 52 tyrosinase activity by microbial source 87 Kailas B. Kumbhar isolated from meat waste Misal P. K., Bendre N. Evaluation of Araneae diversity on PO- 53 N., Pawar P. A., Bhoite Mangifera indica from Lengare, 88 S. H. Maharashtra, India A review on synthesis of zinc oxide S. J. Mane, A. S. Pisal, PO- 54 nanoparticles using plant extracts 89 G. S. Jadhav and their characterization Sawant M. D., Bajabale Efficacy of Withania somnifera in PO- 55 90 P. C., Bhosale P. S. metform in induced aged mice Occurrence and enumeration of P. D. Sagare, N. B. PO-56 arbuscular mycorrhizal fungi in 91 Mane soybean Glycine max (L.) Merr. Komal Pawar, Yogita Effect of seaweed extract on protein PO- 57 92 Patil, S. P. Nalawade profile silkworm of Bombyx mori Sumaiya S. Shaikh, Nitin T. Gore, Abhinav Differential response of local A. Mali, Suraj D. landraces of finger millet (Eleusine PO-58 93 Umdale, Pankaj coracana (L.) Gaertn.) to salinity Mundada, Mahendra L. stress in relation to growth Ahire Study of effect of flower’s extract of M. M. Kandar, S. B. Calotropis gigantea on angiogenesis PO- 59 94 More, R. V. Yadav by Chorio allontoic membrane assay (CAM) in chick embryo S. R. Jaygonawar, T. K. Biodegradation of Cellulosic Waste by PO- 60 95 Ulhalkar, V. S. Patil Aspergillus spp. Bitale Tushar Krushnat, Deshmukh Anil Bioremediation of oil contaminated PO-61 96 Ramchandra, Kate Rahul soil Vitthal, Uttekar G. V.

International E-Conference on “Current Approaches in Life Sciences for Sustainable March 19 & 20, Development” (ICCALSSD-2021) 2021

Koli S. S., Dagade D. R., Median lethal toxicity of fungicide PO-62 97 Bhatia P. R., More R. B. propiconazole on fish Catla catla Antibacterial activity of zinc oxide PO-63 Harshada Desai, nanoparticles synthesized using 98 Dhanraj Nimbalkar Ocimum sanctum against oral pathogens Analysis of Physico-chemical Uttamrao L. Shevale, PO-64 parameters of Kanher Dam, Satara, 99 Sandhya M. Pawar Maharashtra, India P. S. Chavan, R. A. Production of bioplastic by Sontakke., P. D. PO-65 microorganisms isolated from soil 100 Jagdale., S. S. Kanase, sample P. B. Zambare M. D. Sawant, S. S. Present status of fish consumption in PO-66 Magade, V. Y. 101 Satara City Deshpande Ethnobotanical study to determine R. A. Shinde, S. V. PO-67 potential medicinal value of 102 Thigale1, K. B. Pawar Kalanchoe bhidei T. Cooke Uddesh N. Kanase, Effects of Natural fungicide neem oil PO-68 Chaitrali K. Gujar, 103 on Catla catla Vishwas. Y. Deshpande Study of Antimicrobial Activity of Rutuja Rajkumar PO-69 Glycyrrihiza glabra and Cymbopogan 104 Khurape*, C. C. Shete citrarutis Isolation and characterization of Sonal Suresh Mali*, S. S. PO-70 endophytic bacteria isolated from 105 Jangam different medicinal plants Relative toxicity of extracts of two PO-71 S. P. Zanke plant against Corcyra cephalonica 106 (Stenton) under laboratory conditions Isolation, characterization, screening Rutuja Dattatraya and antibiotic sensitivity of PO-72 107 Thorat, Dhumal D. D. Actinomycetes from locally collected soil samples Ghorpade Anuja Screening and isolation of protease PO-73 108 Vijaysinh, U. L. Shevale producing bacteria from soil Amruta Tarade, Salim Isolation of bacterial isolates from rotten PO-74 109 Mujawar fruits for the Production of bioethanol

International E-Conference on “Current Approaches in Life Sciences for Sustainable March 19 & 20, Development” (ICCALSSD-2021) 2021

Low-cost production of Metamerism anisoliae and Beauveria bassiana, Yogini Suresh Lawand, PO-75 Volvariella volvacea for inoculum 110 V. M. Nalawade development (Bioinsecticide) using solid state fermentation In vivo antiangiogenic potential of Sawant M. D., Bendre curcumin nanoparticles using chick PO-76 111 N. N., Deshpande V. Y. chorio-allantoic membrane assay (CAM) Physiological and biochemical Y. R. Chavan and B. A. PO-77 analysis of nine Asteraceae weeds 112 Kore from Satara district Monali J.Pawar, Yogesh Phytochemical Analysis of Figs and PO-78 113 R.Chavan Root exudate of Ficus racemosa. Kashid Yaminee. R., An overview of various aspects for PO-79 Supanekar Green Synthesis of copper 114 Shirishkumar.V. nanoparticles Rutuja Repal*, Riddhi Antibacterial activity of Eclipta PO-80 115 Jog prostrata leaves (L.) Maheshwari Shinde, Isolation and Identification of Plastic PO-81 116 Vishakha Patil degrading bacteria from soil. Akshata S. Takade, U. Biosynthesis of silver nanoparticles PO-82 B. Waghmare from Agave sisalana, characterization 117 and it's antibacterial activity.

Author Name: Priyal Chikhaliwala and Sudeshna Chandra

Institute: Sunandan Divatia School of Science, SVKM’s NMIMS University, Mumbai

Abstract

Nanobiosensor can be miniaturized for real-time detection of target analytes. Nanomaterials can be modified and used as synthetic molecular probes to capture target analytes even in minimum sample volume at low concentrations. On the other hand, Electrochemiluminescence (ECL) is a chemiluminescence phenomenon which is electrochemically generated by applying potential of several volts. This talk will cover development of an electrochemical immunoassay for simultaneous determination of important biomarkers for early detection of hepatocellular carcinoma (HCC). Glypican-3 (GPC-3) is expressed specifically in HCC patients than that in non-cancerous

livers. Magnetite (Fe3O4) nanoparticles (NPs) were decorated and functionalized with hyperbranched amino functionalized dendrimers. The modified NPs were coupled to the antibodies against alpha-feto protein and GPC-3 and their electrochemical behaviour were studied using cyclic voltammetry and electrochemical impedance spectroscopy. The biomarkers can be detected best at voltages of 0.25 mV and – 0.54 mV (vs Ag/AgCl) for AFP and GPC-3, respectively. The low working potentials makes the method more selective over other electroactive species present in real human serum samples. The method was validated by analysing spiked human serum samples. The talk will also feature ECL biosensing of alpha-fetoprotein (AFP) and glypican-3 (GPC-3) using 1 mM tri-propylamine

D5@Fe3O4NP@antibodies as detection probes. A low detection limit for AFP and GPC-3 of 6 pg/mL and 0.03 pg/mL, respectively was obtained. Magnetic

control over the sensing approach helps increase the specificity and sensitivity towards detection process. Together, both the PAMAM dendrimer

and Fe3O4 NPs act in a synergistic way to improve the efficiency of ECL emission and in immunobiosensing.

Biography

Dr. Sudeshna Chandra is currently serving as Professor in the Department of Chemistry, Sunandan Divatia School of Science, SVKM’s NMIMS University. Prior to this, she was research scientist in the department of Metallurgical Engineering and Material Science at IIT Bombay. Dr. Sudeshna did PhD in Chemistry from IIT Roorkee. She received the prestigious Humboldt Fellowship for doing her post-doctoral research at the Department of Inorganic Chemistry at Chemnitz Technical University, Germany. She is also a visiting scientist at University of Regensburg, Germany and a recipient of DST-DAAD fellowship. She also received the “Peter Salamon Young Scientist award” in 2013 awarded by the Telluride Science Research Centre, Colorado, USA. Her research area includes synthesis of nanomaterials and their applications in catalysis, drug delivery, biosensing and imaging. She has 3 patents, 70+ publications in international journals with over 2600 citations and h-index of 26. She is currently handling two major grants from DST Nanomission and DBT.

E-mail address: [email protected]

Title: Agricultural & Rural Distress In INDIA, Global Warming -

Microbiological Facets

Author Name: Shrihari J. Arjunwadkar

Institute: Syner-G Pharma Inc., Winnipeg, CANADA Abstract The agriculture sector and the rural economy in India have been in distress for the last many years. A lot of work has been done to remedy this with limited success. In this presentation, the current problems are discussed. Many other pressing problems and catastrophic environmental issues like the management of solid waste, floods, eutrophication, river cleaning, groundwater replenishment, global warming, etc., will be successfully addressed alongside the main problem. The literature suggests that soil quality in India has considerably deteriorated and is going downhill by the day. The connection between deteriorated/ing soil quality and the various problems mentioned above is discussed. Hence the importance and urgency of correct and decisive action. Grass-root level solutions for addressing the problem are suggested and supported by examples in the literature. In addition to food security, flourishing agriculture is a critical prerequisite for a successful, seamless, and inevitable transition from a petroleum-based economy to a sustainable, bio-based economy (BBE) in foreseeable future. This will open potential diversification possibilities for agricultural commodities that will help achieve price stabilization and a remunerative price proposition. Examples of other possible ways of creating diversification opportunities are listed. In the process, this will provide the potential for job creation. Agriculture’s role in serving societal interests is indispensable in the Indian context. A critical role of microbes in this process is emphasized. This will provide a huge impetus to agriculture and the rural sector of the Indian economy. By doing this, India will be able to achieve “Shashwat Karj Mukti to Kisan” and bring prosperity and respect to the farmer. I am confident that we will solve this problem alongside many others using collective strength, ingenuity, and out-of-the-box thinking. A “Second Green Revolution” is surely achievable using comprehensive, integrated, and multi-pronged approaches.

Biography During my professional career as a process, R&D scientist and Manager acquired extensive experience and expertise in the field of microbiology, fermentation, and process R&D in various settings and scales, from 1 L to 120,000 liters for a range of fermentation products like pharmaceuticals, bio-pharmaceuticals & nutraceuticals, active pharmaceutical ingredients (API), advanced intermediates, vaccines and biofertilizer using a variety of microbes; bacteria, actinomycetes, yeast, and fungi, in regulatory compliant industrial setups. Years of working experience in a regulatory compliant pharmaceutical process industry regulatory affairs and contribute as a consultant. I have authored/reviewed manufacturing documents, technical reports, and regulatory filing – IND, CMC modules, DMF during my professional career as a Subject Matter Expert (SME). Experience in Contract Research & Manufacturing (CRM) operations. Managed multidisciplinary teams effectively in R&D and commercial environments with excellent leadership and project management skills. Managed multiple projects simultaneously and prioritized and maneuvered them in fluid, changing, & challenging commercial environments to maximize returns from available resources and advance corporate projects and goals. Provided strategic and technical inputs. Energetic & self-motivated professional with a track record of critically analyzing data for developing experimental and professional plans. Excellent communication skills for liaising with internal & external clients and stakeholders. Enjoys new challenges and has successfully worked in a rapidly growing, entrepreneurial, multidisciplinary, collaborative team environment. The initiative, flexibility, creativity, & pursuance helped me achieve and surpass milestones & goals - result-oriented and continuous process improvement mindset, vertical & lateral thinking, and ability to establish collaboration. Developed strategies for improvement and implementation at a commercial scale after comprehensive 6M's+ evaluation and regulatory consideration, on time and budgets. A sound understanding of allied fields for advancing process knowledge and develop robust processes. Used statistical Design of Experiment (DoE) for robust process development by improving understanding of key drivers of process performance to deliver quality, cost, and reliability to process operation and product quality since 1993. This is a precursor to Quality by Design (QbD) and Process Analytical Technology (PAT). I troubleshot issues related to customer needs, productivity, & manufacturing. I developed new opportunities by coordinating knowledge, data, critical analysis, & logic as an SME.

E-mail address: [email protected]

Title: Applications of NGS in surveillance of human and animal pathogens

Author Name: Amar Anandrao Telke

Institute: Norwegian Veterinary Institute, Oslo, Norway

Abstract Traditional phenotypic microbiological methods have been using in microbial surveillance. These methods are slow and require prior knowledge of pathogens. Next-generation sequencing (NGS) offers an intriguing solution in microbial diagnostics, as it enables rapid in situ diagnostics through amplicon-based or metagenomics approaches and creates a stream of genomic data that can reveal critical epidemiological aspects of an outbreak or epidemic's dynamics. NGS has many advantages over traditional microbial diagnostic methods, such as unbiased rather than pathogen-specific protocols, ability to detect fastidious or non-culturable organisms, and ability to detect co-infections. One of the most impressive advantages of NGS is that it requires little or no prior knowledge of the pathogen, unlike many other diagnostic assays; therefore for surveillance of pathogen, NGS is very valuable.

Biography Dr. Amar Anandrao Telke, is a molecular biologist, who strongly believe in Science. He is working as senior scientist at section for food safety and animal health, Norwegian Veterinary Institute, Oslo Norway. He has extensive experience in handling and execution of international research projects viz. ARDIG, FED-AMR and Full-Force that are funded by the European Joint Programme (EJP) for One Health. All these projects are related to drug development, antibiotic resistance, and surveillance of pathogens. He has an international patent in technology development. He published more than 35 research articles in international journals that have 1335 citations, 18 h- index, please go through a link to access the publication list https://scholar.google.no/citations?hl=en&user=DmR4bzwAAAAJ&view_op=li st_wor ks&sortby=pubdate.

E-mail address: [email protected]

ORAL PRESENTATION

OR – 01 Phytochemical screening and in-vitro biological activities of leaf extract of Argyreia cuneata Lour.

Sandip S. Kale1, Suresh S. Kale2, Padma B. Dandge1 1Department of Biochemistry, Shivaji University, Kolhapur – 416 004, Maharashtra, India 2Department of Botany, Sathaye College, Vile Parle, Mumbai – 400 057, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT The phytochemical analysis of the plants is very important commercially and has great interest in pharmaceutical companies for the production of the new drugs for curing of various diseases. The medicinal plant Argyreia cuneata L. belongs to family convolvulaceae has been used in different system of traditional medication for the treatment of diseases and ailments of human beings. The chemical diversity of bioactive components and pharmacological actions of many species for genus Argyreia remain unaddressed by rigorous scientific research to define efficacy and safety. The present study is carried out to investigate the qualitative phytochemical constituents and pharmacological actions of the plant. The biological activities such as Antioxidant, Anti-inflammatory, antibacterial & Anti-diabetic of A. cuneata leaf extract are accessed in-vitro by using standard methods. The LC-HRMS analysis of crude methanolic extract of the plant confirmed the involvement of potent bioactive compounds in several biological activities. The study of this plant could be more helpful for identification & evaluation of crude drug for various diseases as it has significant bioactive components. Key words: Argyreia cuneata, phytochemicals, LC-HRMS, Antioxidant, Anti- inflammatory

OR – 02 Ascertaining bacteriological pollution in bottled drinking water

Ivan Aranha Department of Zoology, Ahmednagar College, Ahmednagar, Maharashtra – 414 001 *Corresponding Author E-mail: [email protected]

ABSTRACT Pure and safe drinking water is necessary to lead a healthy life. People generally consider that the bottled water sold commercially is safe for usage for various reasons and it serves as the only source of reliable drinking water in certain situations. Studies on drinking water supplied commercially are most crucial for assessing quality of water with respect to microbial contamination and status of various essential ions. The aim of the present investigation was to assess the bacteriological contamination and physicochemical quality assessment of packaged drinking water sold commercially for public consumption at Ahmednagar City, Maharashtra. Around 35 different brand water bottles were collected from Ahmednagar city for the study. Bacterial contamination study and analysis of physicochemical parameters like pH, TDS, salinity, conductivity and chloride contents was carried out. Total viable count (TVC) was carried out to assess the bacteriological contamination. Out of thirty-five water samples, twelve (34%) water samples were found to be positive for bacterial contamination. The studies of physicochemical analysis of the water samples showed that twenty-seven (77%) water bottle were unsafe for the human consumption. The physicochemical parameters in different brands were not within the standard limits set by WHO for drinking water. These parameters depend on many factors such as natural environment source of water consumption and type of treatment purification techniques applied during the production. Additional changes in water chemistry may occur during the storage and transportation especially when bottles are exposed to direct sunlight. Investigation on bottled water supplied must carried out regularly by the regulatory agencies to ensure safe and nourishing water bottles are supplied for public usage. Key words: Bottled water; Biological contaminants; Chemical contaminants; Drinking water; Physical contaminants

OR – 03 Drought tolerance in Sorghum bicolor under water stress

Ravindra N. Deshmukh Department of Botany and Research Centre, New Arts, Commerce and Science College, Parner, Tal: Parner, Dist: Ahmednagar, (MS), India 4143 302 *Corresponding Author E-mail: [email protected]

ABSTRACT The most popular and economically important rainfed cereal crop Sorghum bicolor (L.) Moench, ranks 1st in the state of Maharashtra, 3rd in India and 5th in the world among the major food crops. Rabi sorghum predominately grown in the states of Maharashtra usually on the residual soil moisture. Rabi sorghum generally suffers from severe moisture stress. This situation totally disturbs the rabi production levels especially on light and medium soils. Amongst all the natural climatic hazards, drought is the single most important disaster affecting food production throughout the world, because 84% of the cultivable lands world over is rainfed. Same is the situation in India, where also 63% of its cultivable land is drought prone. The turgor maintenance through osmotic adjustment is positively associated with yield. The osmotic adjustment positively correlates with growth and yield under water stress. Osmolytes like proline, glycine betaine, free amino acids, reducing sugars and phenols maintain cellular proteins, enzymes, cell organelles and membranes against injury due to water loss. Hence this aspect of screening for drought tolerance in Sorghum cultivars are investigated. For this investigation four promising cultivars i. e. RSV-669, RSV-626, RSV-623 and RSV-629 and one check cultivar RSLG-262 were selected. Overall, in present investigation promising cultivar RSV- 669 showed better performance in accumulation of osmolytes like proline, glycine betaine, free amino acids, reducing sugars and phenols at different levels of water stresses which was followed by RSV-626, RSV- 629 and RSV-623. On the basis of above parameters promising cultivar RSV- 669 and RSV- 626 are drought tolerant and promising cultivar RSV- 629 is moderately drought tolerant and cultivar RSV- 623 is drought susceptible varieties. Key words: Water stress, Osmolytes, Sorghum, Proline, Glycine betaine, Free amino acids, Reducing sugars and Phenols

OR – 04 Validated HPTLC method for estimation of metabolite Embelin from traditional medicinal plants from Myrsinaceae family

Manoj M. Khavate*, Suresh D. Jagtap Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed to be University), Pune *Corresponding Author Email: [email protected]

ABSTRACT Vidanga is traditionally used for the treatment of Colitis and inflammatory disease. The suggested substitute, Embelia tsjeriam-cottam is, used to treat similar conditions. The analysis and quality control of herbal medicines are moving a step ahead towards an integrative and comprehensive direction, in order to tackle the complex nature of herbal medicines. Present study emphasizes on quantification of bioactive secondary metabolites mainly ‘Embelin’ from the fruit extract of Embelia spp, a most important traditional medicinal plant species found around major market of India and from Koyna region, Western Ghats of Maharashtra, India, using high performance thin layer chromatography (HPTLC). The concentration of Embelin in the ethyl acetate extract are E. drupacea (84.74±3.34 mg/ml), E. ribes (112.55±26.88 mg/ml), E. tsjeriam-cottam (779.61±20.12 mg/ml), Bg (4156.20±100.16 mg/ml), Mm (1118.48±314.05 mg/ml), Ng (1626.952±101.88 mg/ml), Pn (4744.45±156.12 mg/ml). E. tsjeriam-cottam showed significantly higher concentration of Embelin compared to other species E. ribes. Current study reported overlays boulevard for E. tsjeriam-cottam to provide a direction for further exploration in precluding clinical study. Key words: Drupacea; Embelia ribes; tsjeriam-cottam; Embelin; herbal medicine; HPTLC; Vidanga

OR – 05 Antidiabetic effect of ethanolic leaves extract of Aegle marmelos on alloxan induced diabetic aged mice

T. A. Gangavane1, R.D. Bodare2, R. B. More3 1Sadguru Gadage Maharaj College, Karad Maharashtra, India 2Prof. Dr. N. D. Mahavidyalaya, Malkapur, Maharashtra, India 3Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author Email: [email protected]

ABSTRACT Aegle marmelos (Bael) is a traditional medicinal plant used in Ayurveda for treating various disease. Now a days one of them most probably occurring disease is diabetes mellitus. In the present study, ethanolic leaves extract of this plant is prepared, phytochemical composition, blood glucose lowering effect of alloxan monohydrate (150mg/kg body wt.) induced diabetic mice were measured and compared with that of a patent drug glibenclamide, preliminary phytochemical screening of the ethanolic and alcoholic leaves extract of Aegle marmelos revealed the presence of tannins, flavonoids, alkaloids and saponins. Mice were administered ethanolic leaves extract of Aegle marmelos at dose 200mg/kg body wt. orally for 15 days respectively. Blood glucose concentration and body weight measured by using GOD/POD method and with electronic weight balance and compared with a patent drug glibenclamide at a dose of 100 mg/kg body wt. the data were compared statistically by using analysis of variance (ANNOVA). The present study indicated that anti-diabetic activity of Aegle marmelos and supports the traditional usage of the herbal preparations for the therapy of diabetes. Key words: Aegle marmelos, diabetes mellitus, alloxan monohydrate, ethanolic leaves extract

OR – 06 Screening of rhizospheric microorganisms for developing effective plant growth promoting bio-inoculant

Deepak U. Bhusare Department of Microbiology, Digambarrao Bindu Arts, Commerce & Science College, Bhokar, Dist. Nanded (MS), India *Corresponding Author Email: [email protected]

ABSTRACT Bio-inoculant (Consortium of Rhizospheric microorganisms) acts as an energy source for the improvement of growth and yield of various agricultural crop in dry area. Bhokar have the hilly region and hence soil doesn’t have an essential microbiota for phosphate solubilization, symbiotic or asymbiotic nitrogen fixation, synthesis of plant growth promoting hormones, siderophore and other biogeochemical activities. Hence due to excessive use of herbicides in the agricultural field they reduce the remaining number of microflora. In present investigation, discovers the existence of Rhizospheric microbiome from the Bhokar agricultural field. Three different agricultural soil samples (Sample A, B & C) were collected and analyzed from Soil testing Department of VNMK University, Parbhani (MS) India. Sample A & B have slight alkaline pH such as 8.08 & 8.33 respectively. High concentration of organic carbon was detected as 1.0% in sample A while average organic carbon was present as 0.58% in sample B. both the samples were analyzed their various parameters such as electric conductivity, concentration of CaCO3, nitrogen, phosphorus, potassium, copper, iron, magnesium and zinc. There are 43 morphologically different species were isolated from the collected soil samples. The selective media were used for the isolation of Phosphate Solubilizing Microorganisms (PSM), Rhizobium sp., Pseudomonas sp., Azotobacter sp., Trichoderma sp. Aspergillus sp. Penicillium sp. All these isolated microorganisms will play a significant role in improvement of growth, crop yield and control the plant diseases in the Bhokar agricultural fields. Key words: Bio-inoculant, Rhizospheric microorganisms, biochemical study, soil samples, PSM

OR – 07 Effect of different media on In vitro seed germination of Embelia ribes Burm F.: a medicinally important liana

Vidya V. Kamble*, Nikhil B. Gaikwad Department of Botany, Shivaji University, Kolhapur, Maharashtra, India *Corresponding Author Email: [email protected]

ABSTRACT Embelia ribes Burm f. (Primulaceae) is a woody liana with poor seed germination. In vitro seed germination was attempted to know the germination percentage and number of days required for germination by using various culture media and their strength. The mature seeds of Embelia ribes were used for the present studies. The seeds were shade dried and stored in the air tight bottle until used. Further the seeds were treated with 0.1% HgCl2 for eight min as per procedure. The seeds with seedcoat and seed without seed coat were inoculated on two different media MS and WPM in full and half strength liquid medium respectively. Since the seed have thick seed coat and morphophysiological dormancy, they have shown poor germination on medium supplemented with solidifying agent. For the present study the media used for in vitro germination is liquid medium without solidifying agent. Highest seed germination is shown by seed with seed coat i.e., 77 ± 0.01% on full strength MS medium within six weeks of culture. 74 ± 0.03 % of germination is shown by seed with seed coat on half strength MS medium. The in vitro seed germination of seed with seed coat on full strength WPM medium has shown 75 ± 0.01% of germination followed by half strength WPM medium with 60 ± 0.02 % germination. The number of days required for germination was 60 days from inoculation. In the present study, in vitro seed germination in Embelia ribes was significantly affected by type of media used for germination. In vitro seed germination was found quicker and well-developed seedlings were observed compared to conventional seed germination. Key words: Embelia ribes, MS medium, WPM medium, In vitro seed germination

OR – 08 Extraction of acid soluble collagen from marine fish waste

Kirdat P. N., Dandge P. B. Department of Biochemistry, Shivaji University, Kolhapur, Maharashtra, India *Corresponding Author Email: [email protected]

ABSTRACT The majority of fish waste was generated from human consumption and industrial by-products of fish. The utilization of this waste for the extraction of collagen has recently become important research topics and also helps to minimize the pollution caused due to waste. The acid soluble collagen was extracted from skin and scale waste material of Sarda orientalis fish. The deproteinization and defatting of fish waste was done by sodium hydroxide and butyl alcohol respectively. The acid soluble collagen (ASC) was extracted at different interval of time using various molar concentrations of acetic acid, lactic acid and formic acid respectively. The primary confirmation of collagen was appearance its gel like structure after centrifugation. The qualitative analysis of collagen was carried out by SDS-PAGE and was quantified by L-hydroxyproline assay. Further the extracted collagen has been studied for its various biophysical characteristics and can be utilized for applications in agricultural, pharmaceutical and food sector. Key words: Sarda orientalis, acid soluble collagen, L-hydroxyproline, SDS-PAGE

OR – 09 Cataloguing bacterial community in the rhizosphere of Shorea robusta Gaertn using portable nanopore sequencer- MinION

Vineet Vishal1,2, Geetanjali Singh2, Shalini Lal2* 1Department of Botany, Bangabasi Evening College, Calcutta University, Kolkata -700 009, West Bengal, India. 2Department of Botany, Dr. Shyama Prasad Mukherjee University, Ranchi - 834 008, Jharkhand, India. *Corresponding Author Email: [email protected]

ABSTRACT The mining and cataloguing of the microbial communities through amplicon-based 16s gene has become the unsurpassed asset in the field of environmental science, ecology and microbiology, not only for research-based activities but also for on-the- spot investigation and monitoring using the Oxford Nanopore Technologies (ONT) MinION portable sequencer. The present study reports the analysis of the rhizospheric microbiome of Shorea robusta Gaertn. from Saranda Reserve Forest Jharkhand, India. Soil samples were collected using standard protocol and 16s rRNA gene V1-V9 region amplicon sequencing was targeted to identify hidden uncultured microbial communities prevalent in the rhizosphere. A total of 28, 239 quality check long reads were obtained and 22,773 reads were matched against RDP 16S/28S reference database implemented under MetaG web-server to reveal the presence of a wide variety of microbial communities with the highest belonging to Proteobacteria superphyla (48.6%), followed Firmicutes (21.8%), Nitrospirae (10.9%), Actinobacteria, Acidobacteria (3.1%), Bacteroidetes (1%), Chloroflexi (1%) whereas Gemmatimonadetes, Crenarchaeote, Cyanobacteria, Verrucomicrobia, Planctomycetes and OTUs unclassified were relatively frequent. The data is available at the NCBI - Sequence Read Archive with accession number: SRX8009932. This metagenomic study provides us a strong foundation for the characterization of unculturable microbial communities existing in this particular niche. Key words: Microbiome, MinIon, RDP, ONT, Saranda, Shorea

OR – 10 Comparative study of solubility of tranquilizers (alternative Date Rape Drugs) in Stomach Acid

Aditya More1, Smitesh Nalage2*, Anita Mali3 Department of Forensic Science, Yashavantrao Chavan Institute of Science, Satara (Autonomous), Maharashtra, India *Corresponding Author Email: [email protected]

ABSTRACT Date Rape Drugs are the members of tranquilizers and sedative class of drugs. These are the substances that make it easier for someone to rape or assault sexually. The person who is administrated by these drugs can cause sleepiness, slow breathing, slow heartbeat rate, trouble in muscle coordination, loss of consciousness, high blood pressure, feel dizziness, and confusion. GHB (gamma- hydroxybutyric acid), Rohypnol (flunitrazepam), Ketamine is generally considered as date rape drugs and heinous crimes were carried out in high numbers using these drugs. As Food and Drug Administration Department of Central Government of India considered this issue and placed these drugs under the schedule of Narcotic Drugs and Psychotropic Substances (NDPS) Act. Thereafter it is nearly impossible to get these drugs easily; as FDA is monitoring its distribution strictly. But criminals have been searched new way through it. Normal tranquilizers and sedatives are also being used for committing such crimes. These drugs are also scheduled, but one can get them with the help of a prescription from a registered medical practitioner. So, we carried out a comparative study of 3 tranquilizers from the benzodiazepine class that are Diazepam, Alprazolam, and Librium to check their dissolving rate in stomach acid. By this, we conclude that at what time drugs started dissolving and started their action on the human body. Estimation of action time of drug can help an investigator to identify the class of drug and time of drug injected (early phase). In our study, we found that the dissolving period of Diazepam and Alprazolam in stomach acid is about 12 to 15 min.

Key words: Gamma-hydroxybutyric acid, Rohypnol, Ketamine, Diazepam, Alprazolam, Librium

OR - 11 Evaluation of growth and protein content in Scytonema schmidtii Gom. in different nutrient media

Janardhan Namdeo Nehul Department of Botany, Dada Patil Rajale College, Adinathnagar Tal – Pathardi, Dist – Ahmednagar, Pin – 414 505, Maharashtra, India *Corresponding Author Email: [email protected]

ABSTRACT Scytonema schmidtii was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959). The axenic culture of Scytonema schmidtii was obtained in the laboratory. For the biomass production, different culture media were used namely BG-11, Fogg’s medium, Allen and Arnon medium, Zarrouk’s medium and CFTRI medium. The biomass was harvested by filtration through double layered muslin cloth and dried using air blower. After harvesting, the biomass obtained was subjected to the growth analysis. Proteins were estimated by following Lowry method (Lowry et al., 1951). Out of the different culture media used, BG-11 medium supported the growth of Scytonema schmidtii properly as compared to other media used. Protein content was found to be more in Scytonema schmidtii grown in BG-11 medium followed by the Allen and Arnon medium. Key words: Scytonema schmidtii, Proteins, BG-11, Fogg’s medium, Allen and Arnon medium, Zarrouk’s medium and CFTRI medium

OR - 12 Mitochondrial gene (mtCOI cytochrome oxidase I) diversity of thrips tabaci infesting onion (Allium cepa L.) in India

Gawai T. B.1,2, Gawande S. J.1, Ade A. B.2, Singh M.1 1Department of Plant Pathology, ICAR- Directorate of Onion and Garlic Research, Rajgurunagar, Pune – 410 505, Maharashtra, India 2Department of Botany, Savitribai Phule Pune University, Pune – 411 007, Maharashtra, India *Corresponding Author Email: [email protected]

ABSTRACT Onion (Allium cepa L.) is one of the most widely cultivated vegetable crops grown in tropical, temperate and boreal regions around the world. On onion, thrips are definite vector for important tospoviruses such as iris yellow spot virus (IYSV) and tomato spotted wilt virus (TSWV). Most of the plant viruses are transmitted by vectors to their host plants. Thrips are globally important crop pests and vectors of viral disease-causing severe damage to plant. Understanding epidemiology of the disease and its vector is important for disease management. Sequence variation in the mitochondrial COI region has proven effective for the identification of species in many groups of insect pests and ultimately useful for the improvement of IPM (Integrated Pest Management) strategies. To investigate the intra-specific genetic diversity of thrips tabaci species, we analysed a 480 bp region of the mitochondrial cytochrome oxidase gene subunit I (COI) sequence variation among 3 localities in India. The study represents the SNP report for the thrips among three localities in India. Key words: mtCOI gene, Thrips tabaci, phylogenetic analysis

OR - 13 Ecological notes on epiphytic fern of northern western ghats of Maharashtra

S. D. Shaikh Department of Botany, Abasaheb Marathe Arts & New Commerce, Science College, Rajapur. Dist: Ratnagiri (MS, INDIA) *Corresponding Author Email: [email protected]

ABSTRACT Epiphyte’s ferns commonly grow on moist and shady tree trunks, branches, tree tops and large shrubs both in dense and partially and completely open forests. The composition and frequency of these epiphytic ferns vary considerably depending upon the altitudes, climatic conditions and nature of forests i.e., nature of bark and shade or open nature of canopy. Usually, conifers do not appear to favour the growth of any epiphytic pteridophytes probably due to resinous bark. But it has been observed at places where sufficient moisture and shade are available, there is a substantial growth of epiphytic mosses and ferns. Epiphyic ferns like Pyrrosia porosa (Presl) Hovenkamp, Microsorum membranaceum (D. Don) Ching, Lepisorus nudus (Hook.) Ching, Araiostegia pulchra (D. Don) Copel. etc. Asplenium ensiforme Wall. ex Hook. & Grev. grow on the tree trunks, branches in forested and open places of several trees especially on Mangifera indica L. Many of the ephiphytes are grow on this plant. It is interesting to note that among the epiphytes, polypodiaceous members are the commonest and most dominant in the present study. Key words: epiphytic ferns, ecology, Western Ghats

OR – 14 Salinity tolerance of Portulaca oleracea L. is facilitated by enhanced oxalic acid synthesis

Vishal V. Naik1*, Amol V. Patil2, Vishal T. Aparadh3, Baburao A. Karadge4 1Department of Botany, Bharati Vidyapeeth’s Matoshri Bayabai Shriptarao Kadam Kanya Mahavidyalaya, Kadegaon – 415 304, Maharashtra, India 2Department of Botany, Dr. Bapuji Salunkhe College, Miraj – 416 410, Maharashtra, India 3Department of Botany, Shri Pancham Khemraj Mahavidyalaya, Sawantwadi – 416 510, Maharashtra, India 4Department of Botany, Shivaji University, Kolhapur – 416 004, Maharashtra, India *Corresponding Author Email: [email protected]

ABSTRACT Portulaca oleracea L. also known as ‘Purslane’, is a C4 succulent belonging to family Portulacacae. Higher concentration of oxalic acid in Purslane is reported. To investigate relationship between salinity tolerance and oxalic acid, one month old plants were treated with increasing levels of NaCl (25, 50, 100 and 200 mM). Results indicated accumulation of oxalic acid followed by enhancement in the activity of enzyme glycolate oxidase in the leaves of Purslane with increase in salinity level. The possible role of oxalic acid and enzyme glycolate oxidase in salinity tolerance capacity of P. oleracea is discussed. Key words: Portualca oleracea, oxalic acid, glycolate oxidase, salinity

OR – 15 Study on removal of viruses using pulsed electrical field

V V Sawant1, V S Sawant2* 1Department of Chemistry, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India 2D. P. Bhosale College, Koregaon, Tal. Koregoan, Dist. Satara, Maharashtra, India *Corresponding Author Email: [email protected]

ABSTRACT The Global impact and economic disruption due to several infectious disease outbreaks in past decades are mainly due to SARS, H1N1, Zika, Bird flu, and the recent outbreak of coronavirus SARS-CoV-2. These viruses are the agent that causes infectious disease. They are organic structure which interact with living organisms. From December 2019, the world is experiencing challenges of pandemic situation caused by coronavirus. The SARS-CoV-2 particles are spherical and have protein spikes protruding from the surface. These spikes attach to human cell, fuses the cell membrane. Treatment and recovery of Covid-19 are still in trials. Vaccination, isolation/quarantine, bio-bubbles are the tools for prevention. Predicting inhibitor and target to the COVID-19 is an urgent need to protect human from the disease. In this view, the available literature on whey protein structural modification and thermal properties induced by both types of pulse electric field (PEF) treatment confirms the protein denaturation of WPI (Whey Protein Isolate). The increase in the electric field intensity, improves protein denaturation of WPI. This paper describes the studies on effect of electric field on structure of protein spikes of corona virus, decreasing the viral load and controls the spread. Such electrical air disinfector systems can be operated at public places like offices, schools, transportation and many more to control the spread of the virus. More efforts should be input into this system to further improve and to use it as efficient viral removal system. Key words: Virus, Whey Protein, Covid-19, Pulse Electric Field (PEF), Denaturation

OR – 16 Influence of plant extract formulation on photosynthetic pigments of bean during rust disease management

Suhas Kundlik Kamble1*, Yuvraj Dhondiram Kengar2 1Department of Botany, Dahiwadi College, Dahiwadi, Tal – Man, Dist – Satara, Pin 415 508, Maharashtra, India 2Department of Botany, Dahiwadi, Smt. Kusumtai Rajarambapu Patil Kanya Mahavidyalaya, Islampur, Dist. Sangli, Pin 415 409, Maharashtra, India *Corresponding Author Email: [email protected]

ABSTRACT Rust of bean caused by Uromyces appendeculatus F. Strauss results in to the severe loss of yield all over the world. The extensive use of fungicides for controlling fungal diseases has adverse effects on environment and human beings too. Moreover, rust pustules reduce the rate of photosynthesis in leaves which ultimately contribute to the yield of bean crops. As disadvantages of chemical fungicides are familiar, an attempt has been taken to control rust of bean by using plant extract formulation, “Panchparni Extract” for the management of rust disease and their positive influence on photosynthetic pigments.

OR – 17 A novel approach of synthesis ZnO nanoparticles from starch: Its characterization and its efficient biomedical applications

Vinaya Sathe1, Prakash Loni2, Prajakta Kamble3, Shradha Khamitkar4, Suresh Suryawanshi1* 1Department of Biochemistry, Shivaji University, Vidyanagar, Kolhapur, Maharashtra, India 2 Environmental Science and Engineering, China University of Geosciences, Wuhan, China. 3Department of Microbiology, Shivaji University, Vidyanagar, Kolhapur, Maharashtra, India. 4V. G. Shivadare College of Arts, Commerce & Science, Solapur, Maharashtra, India *Corresponding Author Email: [email protected]

ABSTRACT This work aims to synthesize zinc oxide nanoparticles using a chemical method. A

ZnO nanoparticles was prepared using zinc nitrate (Zn (NO3)2) and NaOH along with soluble starch as a stabilizer. The obtained fine milky zinc oxide nanoparticles were observed under the light microscope and characterized by UV visible spectrophotometer. Zinc oxide nanoparticles where they were observed as a crystalline, colourless and dispersed form in saline solution. Zinc oxide nanoparticles have received considerable attention due to their unique antioxidant, antimicrobial properties along with the high catalytic and photochemical activity. The antibacterial activity of zinc oxide nanoparticles was checked against Escherichia Coli (NCIM 2662), Salmonella typhimurium (NCIM 5278), Staphylococcus aureus, (NCIM 5276), Bacillus cereus (NCIM 2217), Staphylococcus epidermis, are studied by disc diffusion method. The antioxidant activity was studied using a spectrophotometric assay. All this property along with cell toxicity study was examined by MTT assay which obeys the biomedical application of nanoparticles. The results of this study showed the efficiency of nanoparticles in the progress of biomedical fields. Key words: Antioxidants, Antimicrobial, Callus, ZnO nanoparticle, Escherichia coli

OR – 18 Effect of temperature and composition of fabrics on fabrics stained with blood

Desai S. S., Bhoyar P. R., Kadam S. L. Department of Forensic Science, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author Email: [email protected]

ABSTRACT Blood is a vital biological indicator found on crime scene locations. Blood stains are crucial in reconstruction of crime scene and aid in providing information in cases involving close proximity between victims and suspects. The phenolphthalein (PP)indicator test is used in forensic examination of ascertaining the identity of possible blood stains, which is difficult in case of stains found on fabrics. Current PP test protocols are based on confirmation of blood stains on formation of pink colour due to reaction with erythrocytes, independent of time and nature of the fabric. The current study focused on the effect of time and nature of five selected fabrics viz. terrycot, cotton, silk, jute and khadi. Five bloodstained pieces of each fabric were subjected to PP treatment and change in stain appearance was recorded at variable temperature ranges. Time required for formation of pink colour was observed to increase under ascending temperature points across all the five fabric samples, whereas the nature of fabric caused minor fluctuations in the confirmation. The study shows potential for further modification in the BPA grouping system and improvisation of blood stain testing protocols. Key words: Erythrocytes, bhenolphthalein indicator, blood stains, crime scene, fabric

OR – 19 Significance of Diatoms in drowning deaths

Sushama S. Desai*, Piyusha P. Rajguru Department of Forensic Science, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Diatoms play an important role in diagnosis of death in drowning cases. Diatoms are microscopic unicellular autotrophic algae containing silica and having distinct geometrical shapes. Diatoms have number of characteristic like their widespread availability, high diversity, habitat specificity and have a good potential for preservation and thus this group can be used in forensic analysis for criminal investigations. If the diatoms are present in the medium where drowning occurred, the penetration of diatoms into the alveolar system and blood stream occurs thus diatoms get transported to different organs of body such as bone marrow, brain, kidney, lungs and hard bones (femur). The presence of diatoms inside the body of drowned victim may serve as conclusive evidence in the court. It can be used to figure out place of drowning and primary and secondary crime scenes, to identify cause of death, and time of the year during which death has occurred. Diatom can resist putrefaction, so diatom test is more valuable in cases were decomposition is advanced and post-mortem symptoms of drowning had got diminished. Forensic limnology is a sub field of forensic botany, which examines the presence of Diatom in crime scene sample and victim. Key words: Diatoms; Drowning; forensic limnology; unicellular; bone marrow

OR – 20 AMF associated with bt and non Bt cotton crop from Man tehsil of Satara district (M.S.) India

V. V. Kamble1*, R. B. Yele1, D. V. Rajmane2 1Department of Botany, Dahiwadi College Dahiwadi, Tal- Man, Dist- Satara, (M.S.), India. 2Department of Botany, Mahatma Phule Arts Science and Commerce College, Panvel, NaviMumbai, (Maharashtra), India *Corresponding Author E-mail: [email protected]

ABSTRACT An investigation ofarbuscularmycorrhizal fungi (AMF) diversity associated with BT and Non-BT Cotton (Gossypiumherbaceum L.) Quantification of AMF spores were carried out from rhizosphere and non rhizosphere soil of BT RASI II and Non-BT, Varlaxmi varieties of Cotton. A total of Twenty AMF species belonging to four genera viz. Glomus, Acaulosporaand Scutellospora and Gigaspora were recorded in the rhizosphere soil of Cotton Cv. BT RASI II and Varlaxmi. The isolated AMF species include 12 species of Glomus, 2 species of Acaulospora, 5 species of Scutellosporaand 1 species of Gigaspora. There was a wide range of variation in AM spore distribution in rhizosphere and non rhizosphere soil at 40, 80 and 120 days growth stages of the test crops. Maximum 132 spores were quantified at 120 days stage of rhizosphere soil in Varlaxmi variety. Key words: AMF, Rhizosphere, BT RASI II, Varlaxmi, Man, Gossypium herbaceum

OR – 21 Incidence of leaf blight on Safed Musli

Sachin V. Tawade Department of Botany, D. B. A.C.S. College, Bhokar, Nanded, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Chlorophytum borivilianum Santapau and Fernandes is one of the most important medicinal plants. It is commonly known as Safed musli viz. a traditional medicinal plant belongs to family Liliaceae. Chlorophytum borivilianum has listed as endangered species valued for its fasciculate storage roots. It was reported to have aphrodisiac properties and forms an important ingredient of herbal tonics viz. prescribed in the Ayurvedic systems of medicine. Among the different diseases of Chlorophytum borivilianum, leaf blight caused by Colletotrichum capsici is very severe disease viz. responsible for 30 per cent yield losses. Keeping this in view, the present investigation was under taken with the objective to study the incidence of disease in the cultivation area in Marathwada and Viderbha region of Maharashtra during the seasons in 2007. Diseased plant leaves were collected for isolation, purification and identification of the causal organism. The result showed the incidence of the disease in all cultivated fields, with Colletotrichum capsici. Key words: Incidence, leaf blight, Safed musli, Colletotrichum

OR – 22 Study on Antibiotic resistance profile of coliforms isolated from drinking water resources of Hadgaon Region

Omprakash A. Kadam Department of Microbiology, Digambarrao Bindu Arts, Commerce & Science College, Bhokar, Dist. Nanded (MS). India *Corresponding Author E-mail: [email protected]

ABSTRACT Coliforms are representative indicator bacteria in water, soil and other environments and are often considered as a measure of water quality. They are potentially pathogenic being responsible for causing diseases associated with gastrointestinal infections including typhoid fever, viral and bacterial gastroenteritis, and diarrhea. Water samples were collected from ten drinking water resources at different locations in Hadgaon Region, Maharashtra, India. The physicochemical qualities were studied and physicochemical qualities ranged as follows: pH (6.6 - 7.8), temperature (26 – 30 ºC), turbidity (2.45 – 8.16 NTU), nitrate (0.01 – 0.05 mg/L), nitrite (Trace), sulphate (10.33 – 23.33 mg/L), chloride ions (45.03 – 100.33 mg/L), dissolved oxygen (3.69 – 5.75 mg/L), total dissolved solids (78 – 145 mg/L), total hardness (90 – 520 mg/L) and alkalinity (45.67 – 118.76 mg/L). A total of 11 physicochemical parameters, counts of total coliforms (TC) and Escherichia coli isolates were determined using standard analytical procedures. Confirmed Escherichia coli isolates (n=30) were assessed for virulence associated with Escherichia coli strains causing intestinal and extra intestinal infections. The recovered Escherichia coli isolates were elucidated for their antibiotic profiling by disk diffusion method. While all the isolates tested were susceptible to imipenem, meropenem, amikacin and gatilofloxacin, others were variously susceptible, and resistant as follows; ciprofloxacin (96%), kanamycin (95%), neomycin (92%), streptomycin (84%), chloramphenicol (73%), nalidixic acid (66%), nitrofuratoin (64%), gentamycin (63%), doxycycline (58%), cefepime (57%), tetracycline (49%) and cephalothin (42%). Conversely, all the isolates were resistant to sulphamethoxazole,

Department of Botany, Department of Zoology, Department of Microbiology, Department of Biotechnology and Department of Food Processing and Packaging of Yashavantrao Chavan Institute of 25 Science (Autonomous College), Satara – 415 001, Maharashtra, INDIA International E-Conference on “Current Approaches in Life Sciences for Sustainable March 19 & 20, Development” (ICCALSSD-2021) 2021 and high levels of resistance were equally observed against amoxycillin (59%), ampicilin (57%) and cefuroxime (40%). Present study focusses on investigation of the incidences of colifom in drinking water resources of Hadgaon Region, their MAR index, distribution of species among coliform group, their antibiotics resistance pattern. Key words: Drinking resources, Coliforms, antibiotic sensitivity

OR – 23 Chelate assisted phyto-accumulation of Chromium and its effect on growth, physiological and biochemical responses in the weed, Portulaca oleracea L. in hydroponic conditions

Rohit A. Kale1*, Vinayak H. Lokhande2, Avinash B. Ade1 1Department of Botany, Savitribai Phule Pune University, Ganeshkhind, Pune - 411007, Maharashtra, India Shri Shiv Chhatrapati College of Arts, Commerce and Science, Bodkenagar, Junnar, Pune - 410 502, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Heavy metal pollution and its deleterious effects on environment are crucial and limiting factors for the growth and productivity of crop plants. The absorption of hexavalent chromium [Cr(VI)] by the plants from contaminated sites are vital and severely affecting the growth, developmental and physiological processes of the plants. In the present investigation, attempts have been made on studying the chelate assisted phytoremediation potential of the weed, Portulaca oleracea L. (purslane) for the accumulation of chromium and its effect on growth, physiological and biochemical responses under hydroponic conditions. The rooted stem cuttings of P. oleracea were exposed to full strength Hoagland nutrient solution supplemented with or without optimized concentrations of Cr(VI) (5 ppm) in combination with chelating agents viz., ethylene diamine tetra acetic acid (3mM EDTA), Nicotine amide tetra acetic acid (3mM, NTA), Citric acid (3mM CA), and diethylene triaminepentaacetic acid (1mM DTPA) in hydroponic conditions. Present study revealed that, Cr in absence of chelating agents significantly affected the growth of the cuttings in terms of leaf area, length, fresh and dry weight of root and shoot. However, the growth of the cuttings was significant and positively retained in presence of chromium solution supplemented with chelating agent, NTA in comparison to EDTA, CA and DTPA. EDTA followed by NTA assisted phyto- accumulation of chromium in the cuttings was significantly higher. The enhanced tolerance and reduced toxicity to chromium in presence of chelating agents was

Department of Botany, Department of Zoology, Department of Microbiology, Department of Biotechnology and Department of Food Processing and Packaging of Yashavantrao Chavan Institute of 27 Science (Autonomous College), Satara – 415 001, Maharashtra, INDIA International E-Conference on “Current Approaches in Life Sciences for Sustainable March 19 & 20, Development” (ICCALSSD-2021) 2021 revealed by significantly higher accumulation of proline. Besides, the role of enzymatic (SOD, CAT, APX, and GR) and non-enzymatic (Cysteine, non-protein thiol) antioxidants was found significantly supportive for enhancing the tolerance capacity of stem cuttings to chromium in presence of chelating agents. Overall, NTA was found most effective chelating agent for enhancing the chromium phyto- accumulation and toxicity tolerance potential of P. oleracea under hydroponic conditions. Key words: Portulaca oleracea, Chromium, Chelating agents, hydroponics, Phytoremediation

OR – 24 Visualizing the distribution of silicon in different plant parts of finger millet (Eleusine coracana (L.) Gaertn.) using electron dispersive spectroscopy (EDS)

P. S. Mundada1,2, H. A. Nikule3, S. D. Umdale4, S. Anil Kumar5, V. T. Barvkar1, T. D. Nikam1, M. L. Ahire4* 1Department of Botany, Savitribai Phule Pune University, Pune 411 007, Maharashtra, India 2Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara 415 001, Maharashtra, India 3Department of Botany, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar, Baramati 413 115, Maharashtra, India 4Department of Botany, Yashavantrao Chavan Institute of Science, Satara 415 001, Maharashtra, India 5Department of Biotechnology, Vignan’s Foundation for Science, Technology & Research, Vadlamudi, Guntur, Andhra Pradesh, 522 213, India *Corresponding Author E-mail: [email protected]

ABSTRACT Recent studies demonstrated the beneficial effects of silicon (Si) on the growth and development of many graminaceous plants. Though, exogenous application of Si on growth, yield and nutrient status is not known for finger millet (Eleusine coracana (L.) Gaertn.). In the present study, the efforts were made to assess the localization of Si in different plant parts of the finger millet. In addition to this, the attempts were made to evaluate the effect of exogenous application of Si on growth, yield, and nutrient status of finger millet. Finger millet readily absorbs the Si and accumulate it in the aerial organs, predominantly in leaves. Exogenous supplementation of silicon significantly improved all the attributes of plant growth and yield. Si supply alters the status of significant nutrient such as carbon (C), nitrogen (N), and oxygen (O) in different plant parts studied. There is a negative correlation between Si content and C content in all the plant tissues examined. Silicon has got slight positive or no impact on the accumulation of nitrogen and oxygen content in different plant tissues. To the best of our knowledge, this is the first report on Si accumulation and its influence on growth, yield, and mineral accumulation such as carbon, nitrogen, and oxygen. Key words: Carbon, EDS, Finger millet, Nitrogen, Oxygen, Silicon

OR – 25 Cypermethrin persuaded instabilities of digestive enzymes in Labeo rohita

V. Y. Deshpande*, M. D. Sawant, P. S. Pawar Department of Zoology and Fisheries, Yashavantrao Chavan Institute of Science, Satara 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Now a days synthetic pyrethroids are preferred due to their high toxicity to insect pests and relative low cost. Cypermethrin is a most potent pyrethroidinsecticide. Pyrethroids are synthetic compounds structurally derived from pyrethrin, one of the active components of pyrethrum. Pyrethrum is extracted from the dried flower heads of Chrysanthemum cinerariaefolium. The pesticide cypermethrin is used worldwide to control various pests in agricultural, domestic and industrial fields. Though the pyrethroids are the potent insecticides, they deteriorate the quality of water and subsequently causes detrimental effects on non-target organisms and can be fatal to them. Many insecticides are sparingly soluble in water but even at very low concentrations they have hazardous effects on aquatic non-target organisms and they represent the highest trophic level in the aquatic food chain. Therefore, persistent pesticide and their residues accumulate to a maximum concentration in thebody of aquatic organisms when compared to other organisms. The fishes are good bio-indicator of pollution. So, the present study was aimed that to evaluate alteration in digestive enzymes of fish, Labeorohita after chronic (30 days) exposure to cypermethrin. The fish, L. rohita exposed to cypermethrin at 1/10th and 1/20th of LC50 concentration for 30 days. After exposure it was observed that enzymes such as amylase, protease and lipase activity fluctuated significantly in intestine and intestinal bulb. There was decrease in amylase activity except in intestinal bulb at l/20th of LC50, protease activity decreased in intestinal bulb and increased in intestine whereas decrease in lipase activity was more in intestine than in the intestinal bulb. These fluctuations in the enzymes might be due to the pesticide intoxication. It seems evident from the results of the present study that fishes generally survived for long periods at sublethal concentrations but, tissue and organ damage was inevitable. The study also suggests that if, water pollution hazards are to be evaluated, biochemical fluctuations and tissue degeneracy is much effective criterion than number of mortality. Key words: Pyrethroid insecticides, chronic, enzymes, fluctuations

OR – 26 In-vitro rooting and hardening of excised shoots of Momordica cymbalaria Hook. f.

S. V. Madhale Department of Botany, Shri. Yashwantrao Patil Science College, Solankur, (Radhanagari), India *Corresponding Author E-mail: [email protected]

ABSTRACT In vitro culture technique is now days becoming a popular tool for the conservation of rare and endemic plants. In the family cucurbitaceae in vitro propagation of different members has been reported by several researchers. The plant growth regulators like NAA, 2-4 D, BAP, and IBA are playing major role in tissue culture technique. In the present research work focus is given on rooting and hardening of excised shoots by using NAA and IBA in different concentrations for the in-vitro culture of M. cymbalaria. Attention is concentrated on development of rapid protocol for rooting and hardening. Key words: Rooting, Hardening, Excised shoots, in-vitro culture

OR – 27 In vitro multiplication and assessment of genetic stability of Ceropegia lawii Hook. An endemic and critically endangered medicinal plant of Western Ghats

Mukta Rajaram Bhamare1*, Hemantkumar Atmaram Thakur2 1Department of Botany, K.T.H.M College, Nashik – 422 02, Maharashtra, India 2Department of Botany, H.P.T. Arts and R.Y.K Science College, Nashik – 422 005, Maharshtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT The aim of present study was to optimize direct shoot organogenesis (DSO), Indirect shoot organogenesis (ISO) and efficient protocol developed for rapid in vitro multiplication of an endemic and critically endangered medicinally potential herb Ceropegia lawii. Effect of various phyto hormones Benzylamino purine (BAP), Kinetin (KIN), Thiadizurol (TDZ), Indol Acetic Acid (IAA), Indol Butyric Acid (IBA), Naphthalene acetic acid (NAA) & 2, 4, Dichlorophenoxy acetic acid (2,4-D) on in vitro regeneration were investigated. The seeds were germinated on half strength MS basal medium and developed plantlets. The cotyledonary nodes and cotyledons were used as source of explants in DSO & ISO respectively. The best response for callus induction and proliferation was observed in 1.5 mg/lit NAA in which 98.5% of cultured produced an average fresh weight 1.230 ± 0.6 gm (Fresh Weight) of callus. The maximum frequency of shoot (96.66%) with 18 ± 0.4 shoot per explants was observed. The longest shoot length 11.6 ± 0.8 cm were noted. The highest frequency of shoot obtained (88.66%) for ISO reported were Calli transfer on Murashige and Skoog medium supplemented with 2.5 mg/lit BAP and 0.5 mg/lit IBA. Longest shoot length 9.8 ± 0.8 cm were obtained. Transferred in vitro raised shoot to half strength ½MS medium with 1.5 mg/lit IBA shows highest root 7 ± 0.3 were reported. Maximum root length 3.8 ± 0.6 cm was noticed. The rooted in vitro plantlets were transfer in mixture of sterile soil, sand & Coco peat (1:2:1) successfully acclimatization in green house. The present study has established the rapid micropropagation protocol. It will be simple, coast effective and restoring of constrain population of endemic and critically endangered, threaten C. lawii. Micropropagated plantlet subjected to internal simple sequence repeats (ISSR) marker-based profiling reveled genetic fidelity between micropropagated plantlets and mother plant. Key words: Rooting, Hardening, Excised shoots, in-vitro culture

OR – 28 Neurotoxic effect of DDVP on economically important freshwater fish Pungasius bocourti (Sauvage, 1880)

Shelar A. S., Suryawanshi P. S., Pawar P. S.* Department of Zoology and Fisheries, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Water is essential for survival of every living thing. Aquatic resources such as pond, rivers, streams, lakes etc. pure and healthy water to people. Water pollution and toxicology and effects of toxicants on non-target organisms is an important topic of discussion now days. The use of pesticide is increasing day by day for welfare of human but it has detrimental effects on environment as well as human being itself. DDVP is a potent organophosphate pesticide and widely used in all fields. It having high toxicity index. Present study was aimed that neurotoxic effect of pesticide DDVP on fresh water fish, Pungasius bocourti. In present research work fishes were exposed to 1/10th of LC50 conc. (20 ppm) i.e., 2 ppm of dichlorvos for 30 days. The acetylcholinesterase, LH and FSH was estimated in Control and pesticide exposed fishes. Significant depletion in acetylcholinesterase, LH and FSH was observed in exposed fishes. So, the DDVP elicited many significant alterations in fish physiology and metabolism. Key words: Organophosphate, acetylcholinesterase, LH, FSH, depletion

OR – 29 Development and sensory evolution of Amaranth chocolate

Ranvare Abhishek Rajendra, Pandurang Dure* Department of Food Processing and Packaging, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT The Sensory & analytical study of Amaranth Chocolate was carried out. The main objective of Amaranth Chocolate is to control hunger. Chocolates are act as Functional food, in preventing in diseases like Coronary heart disease & hypertension. Chocolates are also helpful in memory improvement. The main ingredients of Amaranth Chocolate are Amaranth, Puffed rice, Dates & Dark chocolate. The Amaranth is one of the pseudo cereals with high amount of protein compared with other cereals. Amaranth & Puffed rice are available in bulk form in the satara market. The dates in Amaranth chocolate give sweet test. Amaranth & Dark Chocolate have same health benefits like managing the blood cholesterol level & hypertension conditions. Key words: Development, Amaranth, Formulation

POSTER PRESENTATION

PO – 01 Effect of occupational environment on respiratory health of female workers in brick industry

Irumjahan Khan1, R. B. More2 1Department of Zoology, S. G. M. College (Autonomous), Karad, Dist - Satara, Maharashtra, India 2Department of Zoology, Yashavantrao Chavan Institue of Science (Autonomous), Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Bricks are still produced by the old traditional manual methods in many parts of India. Brick industries in India provides source of livelihood to millions of people but it is also considered as a highly polluting industry by pollution control board. High concentration of dust, particulate matter and smoke is given out from the brick industry which is not only harmful to environment but also affects the health of brick workers badly. Workers inhale these harmful particles which enter their respiratory system ultimately affecting their lung performance. Present study was conducted on female workers of brick industry in Karad. Study area showed open clamp kiln which releases high concentration of particulate matter (SPM, PM10 and

PM2.5) and gases like SO2 and NO2 in the working environment. Spirometric assessment of lung function showed reduced values of FVC, FEV1, FEV1/FVC and PEFR when campared with the control values. Reduced flow rates indicate the restrictive and obstructive respiratory problems in female workers due to saturation of dust particles in the upper and lower airways. Assessment of lung function of female workers of brick industry confirmed the prevalence of respiratory stress and respiratory health problems. Key words: brick industry, particulate matter, lung performance, respiratory stress

PO – 02 Isolation of Pseudomonas sp. from rhizospheric soil and polluted water for determination of the PGPR (plant growth promoting rhizobacteria) activity along with its applications

A. A. Gangawane, N. T. Pawar, A. U. Sutar* S. G. M. College, Karad – 415 124, Dist. Satara, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Attempts were made to isolate Pseudomonas sp. from rhizospheric soil of pigeon peas, cowpeas and groundnut plants. It was isolated from polluted water also and total four isolates were tentatively identified as P. fluorescens based on morphological and biochemical characters. All the isolates showed direct and indirect plant growth promoting traits. In this study, all four isolates were screened as potential biological control agents against different plant diseases such as Lokri mawa on sugarcane leaves, Kala (black) mawa and powdery mildew on broad bean. The mechanism of Pseudomonas isolates viz., volatiles (HCN) and antibiotic production tests were studied and observed for antibiotic and HCN production. The isolates also showed broad spectrum in vitro antibiotic activity against additional pathogens. It was able to produce considerable amount of indole-3-acetic acid (IAA). The isolates also carried a function of phosphate solubilization. The cellulose based formulations of all isolates were prepared and the bio-efficacy was tested separately under in situ as well as field conditions. Hence, these isolates can be used as potential biofertilizers. Key words: Pseudomonas sp., PGPR, Indole acetic acid, biological control agents, biofertilizer

PO – 03 Integrated nutrient management and maintaining soil fertility

Mayur S. Deshmukh1*, Dipti D. Ghadge2 1 Department of Chemistry, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India 2 Department of Forensic Science, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT In the past, people used farmyard manure and vermicompost in the fields, so the income from agriculture was high and of good quality, but after the Green Revolution in 1960, India started using chemical fertilizers in large quantities This started to increase the yield but the biggest effect was on the soil. Given the current state of Covid-19 pandemic, many people in the city have lost their jobs and so many people have come to the villages and turned to farming. Chemical fertilizers are being used extensively to make more profit. At present, turning directly to organic farming is not possible. When we did experiment with mixed fertilizer on the eggplant plant in the field, we noticed that, higher and better yield can be obtained and mixed fertilizer can also be the better and effective option for better yield. In this way we can gradually reduce the use of chemical fertilizers and turn to organic farming as a whole.

PO – 04 Study of morphology with respect to shape and size of pollen grain (micrometry) from family Malvaceae and Apocynaceae

D. P. Patil*, V. B. Chopade Department of Botany, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT In nature numbers of flowering plants are present. These all-flowering plants are categorized into number of families. The classifications of flowering plant are based on number of characters including vegetative and reproductive character. One is out of that is pollen character like shape and size. The pollen characters are also important for study of allergies, historical geology and paleontology, forensic science, archeology. Malvaceae and Apocynaceae are two major families with variation in flowers as well as pollen characters. So present study is deals with pollen size and shape associated with Malvaceae and Apocynaceae families. Key words: Malvaceae, Apocynaceae, Pollen grain, Micrometry

PO – 05 Studies on judicious doses of nitrogen fertilizer and biofertilizer on yield performance of fodder crop maize (cv. Ganga Safed - 2)

Bendre K. B. Nanasaheb Y. N. Chavan Arts Commerce and Science College, Chalisgaon 424 101, Dist. Jalgaon, (MS), India *Corresponding Author E-mail: [email protected]

ABSTRACT Use of inorganic fertilizer has become essential part of the crop production and a balance form of fertilizer use is always a prerequisite to obtain the higher yield. However, these fertilizers are costly and also pollute the environment through the process of denitrification and volatilization and soil water through leaching wherein only 50 percent of available nitrogen is being used and rest 50 percent goes as waste and is an environmental hazard. Hence, a strategy for integrated nutrient supply is evolved by using judicious combination of chemical fertilizer, organic manure and biofertilizers (Panwar et al., 2001). Therefore, a combine effect of chemical fertilizer along with biofertilizer on percentage increase in yield of fodder crop maize (cv. Ganga safed- 2) and saving of nitrogenous fertilizer due to the use of biofertilizer was studied under present investigation. Key words: Nitrogen fertilizer, Biofertilizer, Yield, Maize, Integrated dose

PO – 06 Study of phytoplankton and hydrobiology in water bodies of Satara city

A. R. Bhosale*, S. S. Gaikwad Department of Botany, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT The present work deals with study of hydro biological studies and diversity of phytoplanktons of fresh water bodies from Satara city. Hydro biology deals with physico chemical factors such as temperature, hardness, pH and turbidity of water. Comparative study of two local water bodies in Satara, Mangalwar Tale and Jalmandir Tale was done. Four genera of phytoplanktons were reported from water bodies under study. From study, it was concluded that phytoplanktons has a crucial role in determination of physicochemical properties of water bodies. Key words: Phytoplankton diversity, Mangalwar tale, Jalmandir Tale

PO – 07 Effects of pesticides on phyto constituents of vegetables and fruits in Sangli and Satara District

S. B. Ghorpade*, S. A. Shinde-Inamdar Department of Botany, Yashavantrao Chavan Institute of Science, Satara 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Fruit and vegetables get attacked by pests and diseases during their production and storage. Farmers are used pesticides, in order to reduce the loss by pests and diseases also maintained the quality. Farmers use of pesticides. However, these pesticides remain on the fruits and vegetables in the form of residue. These pesticidal residue are found to harmful and causing harmful effect on human beings, and environment. This project is chosen depending on the above reason. These pesticidal residue from fruits and vegetables were detected by various method i.e., HPLC and Gas Chromatography. After detecting the pesticidal residue from grapes, 2 types of chemicals were found i.e., Chlormequat Chloride and Chlorpyrifos. Also, from Brinjal, Dicofol was detected. Distinct phytochemical test was also performed. However, this project will bring awareness the farmers about the adverse effects of pesticides. Key words: pesticidal residue, vegetables, fruits, phytochemical

PO – 08 Analysis of microbial diversity from soil samples of various habitats through DNA sequencing

Bagal R. B.1, Bodake V. A.1, Bendre N. N.2, Pawar P. A.3, Wadikar S. S.1, Khedakar G. D.4, Bhoite S. H.5 1Department of Microbiology, Yashavantrao Chavan Institute of Science, Satara, Maharashtra, India 2Department of Zoology, R. B. Narayanrao Borawake College, Shrirampur, Maharashtra, India 3Department of Zoology, Dada Patil Mahavidyalay, Karjat, Maharashtra, India 4Deptartment of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India 1,2,3,5Drongo (NGO), Nisarga Sanvardhan va Sanrakshan Sanstha, Satara, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT An increasing interest has emerged with respect to the importance of microbial diversity in soil habitats. The extent of the diversity of microorganisms in soil is seen to be critical to the maintenance of soil health and quality, as a wide range of microorganisms is involved in important soil functions. The study was initiated to decipher the diversity of soil microbial communities using 16s rDNA sequencing technique to investigate soil microorganisms at finer level and further identified by downstream bioinformatic analysis. Therefore, soil samples were collected from various habitat like grassland, teak farm field and paddy field located at Mahadare forest from Satara district and cultured through serial dilution technique and further identified by 16s rDNA sequencing. A total of 14 microbial species were isolated among which Bacillus genus was most dominant followed by Auriococcus, Xylophilus, Variovorax respectively. From this we can conclude that, presence of above microbes signifies that the soil possesses good soil fertility and provides essential requirements for maintaining soil heath and quality for proper vegetation in Mahadare forest of Satara. Key words: Microbial diversity,16s rDNA sequencing, habitats, bioinformatics

PO – 09 Floral diversity analysis of Kushi village and adjoining areas

N. B. Salunkhe*, S. M. Deshapande Department of Botany, Yashavantrao Chavan Institute of Science Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Floral diversity refers to diversity of naturally occurring indigenous plants of particular region in particular time. The floral diversity helps in the assessments of biodiversity in terms of plants. In the present investigation floristic components of Kushi village from Satara district are enumerated. A total of 70 taxa belonging to 44 families were enlisted during different seasons of the year. Among 44 families Fabaceae (3), Malvaceae (6), Caesalpiniaceae are dominant in the study region. Among the studied taxa (23) are herbs, (14) shrubs, (7) climbers and (24) trees. Delphinium malabaricum (Huth) Munz. endemic species is found. Key words: Kushi, Satara, floristics

PO – 10 Zooplankton diversity of Uttarmand reservoir in Patan Tahsil, District Satara (M. S.) India

Gaikwad V. D.1*, More R. B.1, Padule A. R.2 1P. G. Department of Zoology and Fisheries, Yashwantrao Chavan Institute of Science, Satara (M. S.) India (Autonomous) 2Department of Zoology, Yashwantrao Chavan College of Science, Karad, Maharashtra, India Corresponding Author E-mail: [email protected]

ABSTRACT Zooplankton are the smallest organisms present in any aquatic body. They float, drift or weakly swim in the water. Zooplankton are vital components of freshwater food web and contribute to biological productivity. Zooplankton acts as main sources of food for many fishes and plays an important role in early detection and monitoring the pollution of water. Variation in water quality such as changes in nutrient levels, conductivity, temperature or pH can lead to changes in species composition and abundance. Uttarmand reservoir is a medium irrigation project constructed across the river Uttarmand, a tributary of Krishna river in Patan Tahsil of Satara district in Maharashtra. The present investigation deal with study of zooplankton diversity of Uttarmand reservoir to check the status in the area and provide new insights into its ecology. Key words: Uttarmand Reservoir, Zooplankton, food web, water quality

PO – 11 Assessment of arbuscular mycorrhizal fungi and soil characterization of Caralluma fimbriata Wall.

P. S. Jadhav, N. B. Mane* Department of Botany, Yashavantrao Chavan Institute of Science, Satara (Autonomous), Maharashtra, India *Corresponding Author E-mail: [email protected] [email protected]

ABSTRACT Arbuscular mycorrhiza (AM) is ecologically important type of mycorrhiza which is most widely commercially exploited in agriculture/forestry. In present investigation occurance of Arbuscular Mycorrhizal Fungi (AMF) was investigated from the rhizosphere of Caralluma fimbriata growing area in Satara District. The rhizosphere soil was collected and using saplings of Caralluma fimbriata for a period of vegetative growth phase of plants i.e. After 40 days. The species was studied with isolation of the AM fungal spores from the rhizospheric soil. The spores were occurred in soil after 40 days period. On the basis of morphological characteristics, a total of ten AMF species belonging to three genera viz. Glomus, Aculospora and Sclerocystis were identified. The occurrence of AMF species was showing fungal hyphae, with globose and subglobose structure. Among Glomus geosporum, Glomus intraradices, Acaulospora and Sclerocystis genus was found but Glomus was found dominant Key words: Arbuscular Mycorrhiza, Caralluma fimbriata, Glomus, Acaulaspora, Sclerocystis

PO – 12 Arbuscular Mycorrhizal Fungi associated with variety of Crotolaria pallida Aiton. found in drought prone area of Shendre area in Satara District

S. T. Wagh, N. B. Mane* Department of Botany, Yashavantrao Chavan Institute of Science, Satara (Autonomous), Maharashtra, India *Corresponding Author E-mail: [email protected] [email protected]

ABSTRACT Arbuscular Mycorrhizal Fungi are the key component of soil microflora. They are used as best biofertilizers used to decrease input of fertilizers and chemicals for sustainable agriculture, among soil micro-organisms arbuscular mycorrhizal fungi could promote plant development and controls plant fungal diseases. Arbuscular Mycorrhizal Fungi were beneficial for environmental conservation for yield of crop plants. Keeping in view, the research work carried out the occurrence and its distribution in growth of Crotolaria pallida. The screening of rhizospheric and non- rizospheric soil showed in 11 species of arbuscular mycorrhizal fungi. Total arbuscular mycorrhizal fungal species in two genera Glomus was dominant. Arbuscular Mycorrhizal fungi varied significantly in Crotolaria pallida influenced by soil edaphic factors. Percent root colonization was found more in vegetative phase of grown plant. Key words: Arbuscular Mycorrhizal Fungi, Crotolaria pallida, edaphic, Glomus

PO – 13 Allelopathic effects of two common weeds on germination and seedling growth of two seasonal crops in Satara district

Shivani S. Gaikwad, Abhinav A. Mali* Department of Botany, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Allelochemicals released by a plant can have stimulative as well as inhibitive response to certain neighboring plants. Laboratory experiments were carried out to understand the allelopathic effects of two weeds Cynodon dactylon L. and Parthenium hysterophrous L. on growth performance of two crop plants Triticum aestivum L. and Arachis hypogaea L. respectively. The crop seeds were treated with distilled water and weed extracts of 25 %, 50 %, 75 % and 100 % concentartions and germinated on a double-layered germination paper in petri plates. Effect of treatment was analyzed with respect to germination percentage, root and shoot length of crops seedlings. It was observed that treatment of weed extract shows stimulatory effect on germination percentage, root and shoot length. Germination percentage of both crop plants was enhanced after treatment of weed extract of 75% concentration. Among all weed extract concentrations, 75% concentration of C. dactylon L. and 25% concentration of P. hysterphorous L. shows maximum stimulation with respect to seedling growth of crop plants. Key words: Allelopathy, Cynadon dactylon L., Parthenium hysterphorous L. Arachis hypogaea L., Triticum aestivum L., seedling growth

PO – 14 Effect of pomegranate juice and peel extract on dimethoate toxicity studied on protein profile in liver and kidney of aged mice

C. B. Jamdade1*, R. D. Bodare2, V. Y. Deshpande3, R. B. More3 1Sadguru Gadage Maharaj College, Karad, Maharashtra, India. 2Prof. Dr. N. D. Patil Mahavidyalaya, Malkapur, Tal. Shahuwadi, Dist. Kolhapur, Maharashtra, India. 3Yashavantrao Chavan Institute of Science, Satara, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Aging is the most significant emerging demographic phenomenon in the world today. Aging is accompanied by declines in cellular proteolytic capacity of organs. Decrease in protein synthesis is a general phenomenon observed in the aging. Dimethoate (O-O diethyl 5-methyl carbonyl methyl Phosphorodithioate) an organophosphate pesticide used in controlling many pests on variety of crops. Punica granatum is the Ancient fruit used on many health issues. It has great medicinal interest and rich source of antioxidants due to high level of Phytochemicals. The aim of this study was to examine the effect of pomegranate juice and peel extract on oxidative stress induced dimethoate on protein profile in liver and kidney of aged mice. Male Swiss albino mice, Mus musculus was used for the present investigation, animals were divided into 4 groups in an environmentally controlled condition for 20 days. The group I was served as control group (0.5 ml distilled water) orally, Group II received dimethoate orally (15 mg /kg b.w.), Group III received a. Juice (5 ml/kg b.w.) and b. Peel (200mg/kg b.w.) and Group IV received a. Dimethoate and Juice b. Dimethoate and Peel. The effect of pomegranate juice and peel extract was evaluated against aging. The protein content in the liver and kidney was recorded. The protein content was significantly reduced after toxicity of dimethoate during aging, after pomegranate juice and peel extract treatment in groups IV it was again increased significantly. Thus, pomegranate juice and peel extract is able to ameliorate the stress induced changes in protein during aging. Protective effect of pomegranate juice and peel extract in liver and kidney of mice might be due to its antioxidative property. Key words: Dimethoate toxicity, Pomegranate peel, Protein, Punica granatum

PO – 15 Synergistic effect of agrochemicals (herbicides) on development of streptocycline resistance in Xanthomonas axonopodis

V. B. Chopade Department of Botany, Yashavantrao Chavan Institute of Science (Autonomous), Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Pomegranate having high medicinal value so valuable for human being. Maharashtra is the leading producer of Pomegranate in the country and accounts for 66.2% of the total production of pomegranate in the country. Pomegranate plant having various economic important but it get infected due to the various fungal and bacterial diseases. Large scale infestation of Bacterial Blight disease caused due to Xanthomonas axonopodis pv. Punicae has resulted in considerable damage to the crop from 2006-07. The studies on the effect of streptocycline with different agrochemicals will help in the management of the disease. Key words: Pomegranate, Bacterial Blight, Xanthomonas axonopodis

PO – 16 Proximate composition and phytochemical analysis of different plant parts of Vigna stipulacea Kuntze

Nitin Gore1, Kajal Patil1, Sandhyarani Khilare1, Sumaiya Shaikh1, Hanmant Shinde1, Abhinav Mali1, Pankaj Mundada2, Suraj Umdale3, Mahendra Ahire1* 1Department of Botany, Yashavantrao Chavan Institute of Science, Satara 415001, Maharashtra, India 2Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara 415001, Maharashtra, India 3Department of Botany, Jaysingpur College, Jaysingpur 416 101, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT In the present study, we evaluated the proximate composition and phytochemical analysis of the crop wild relative Vigna stipulacea Kuntze. The seeds are edible, and some local people cultivate it mainly as pasture but sometimes as food. Therefore, in the present study the efforts were made to evaluation of nutritional status of edible parts of the plants. The proximate composition analysis of different plant parts such as leaf, stem and seeds were determined using methods as described by AOAC. Total phenolics, flavonoids, alkaloids, and tannins content were evaluated from the aqueous extracts of leaf, stem and seeds. Leaves exhibits the higher moisture content (84.00±0.91) whereas seeds showed least moisture content (9.25±0.25). Stem of the plants showed considerable amount of ash content (10.00±0.00) followed by leaves (5.67±0.33) and seeds (4.67±0.33). Among the different plant parts, seeds are found to be a rich source of total carbohydrates, crude proteins and free amino acids. Seed extracts showed higher amount of total flavonoid content (TFC), total alkaloid content (TAC) and total tannin content (TTC) whereas, total phenolic content (TPC) was found to be more in leaf extracts. The results of the present study demonstrates that the seeds are rich source of proteins, carbohydrates and phytochemical composition. Key words: crop wild relative, Vigna stipulacea, TPC, TFC, TAC, TTC

PO – 17 Comparative account of micro-morphological features among different accessions of Jasminum multiflorum (Burm.f.) Andr. (Oleaceae) from Satara, Maharashtra (India)

Sonal D. Shindea, Swapnaja M. Deshpandea, Sunil H. Bhoiteb, Jaykumar J. Chavana,* aDepartment of Botany, Yashavantrao Chavan Institute of Science, Satara – 415001, India bDRONGO- Nisarga Sanshodhan Sanvardhan Sanstha, Satara – 415002, India *Corresponding Author E-mail: [email protected]

ABSTRACT Jasminum multiflorum (Burm.f.) Andr. is an evergreen shrub belonging to family Oleaceae. It has ornamental potential because of showy attractive fragrant flowers in capitate cyme. The species shows phenotypic variations in some characters which can be of taxonomic significance. In the present investigation eight different accessions were observed for morphological variations in vegetative and floral characters from Satara district of the Northern Western Ghats of India. The anatomical studies of aerial parts of J. multiflorum revealed the noteworthy differences in arrangement of vascular bundle, epidermal hairs and stomata. Key words: Jasminum multiflorum, Accessions, Satara, morphological features

PO – 18 TiO2 nanoparticles synthesis by an isolated fungus

A. A. Survase1, Shivangi Shivraj Kanase2* 1Rayat Institute of Research and Development (RIRD), Satara – 415 001, Maharashtra, India 2Department of Microbiology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT

Microbial synthesis of TiO2 nanoparticles was achieved by using an isolated fungus from soil ecosystem. A simple, facile, eco-friendly and cheaper approach or protocol or method has been used for the synthesis. TiO2 nanoparticle formation was monitored by visual observation, UV-Vis spectroscopy, FTIR, XRD, FESEM and FETEM studies. Visual observation showed white precipitate formation within 48 hours at 270C, 150 rpm. UV-Vis spectroscopy showed maximum absorbance at 214 nm. FTIR spectra revealed presence of functional groups indicating involvement of biomolecules in capping process. XRD studies showed peaks at 25.190, 38.530, 48.040 and 53.860 respectively indicates pure anatase tetragonal crystal form of TiO2.

FESEM revealed spherical shape and size of TiO2 nanoparticles ranging from 20nm to 45nm. EDAX results showed elemental composition of TiO2 contain titanium 84.18% and 15.82% of oxygen. FETEM image showed lattice spacing size and interplanar distance 0.37 nm. TiO2 nanoparticles immense applications in bioremediation, solar cell, agriculture etc.

Key words: TiO2 nanoparticles, UV-Vis spectroscopy, FTIR, XRD, FESEM

PO – 19 Zinc induced alternations in protein, glycogen and lipid contents in tissues of freshwater fish Gonoproktopterus kolus (Sykes)

Karanjkar D. M.*, V. Y. Deshpande P. G. Department of Zoology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT In acute toxicity (96 hr.), experiment fingerlings of freshwater fish

Gonoproktopterus kolus (Sykes) were exposed to zinc sulphate (Zn SO4). The LC0 and LC50 concentrations were 2.430 ppm and 4.860 ppm respectively. Various tissues like gill, liver, muscle, kidney and intestine were obtained separately from control, LC0 and LC50 groups. Protein, glycogen and lipid from these tissues were estimated by using standard methods. The glycogen content in all the tissues decreased considerably as compared to control group. The total protein content decreased in all tissues except, kidney in LC0 group. Total lipid content decreased in all tissues after acute exposure, as compared to control group. It was observed that, acute toxicity to fish Gonoproktopterus kolus exposed to Zinc sulphate caused depletion in biochemical composition in various tissues. Key words: Gonoproktopterus kolus, acute toxicity, Zinc sulphate, Glycogen, Protein, Lipid

PO – 20 Comparison of sustainable methods of water purification: seed extract and charcoal

Dipti D. Ghadge, Mayur S. Deshmukh, Smitesh Nalage*, S. T. Mahanwar Department of Forensic Science, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Water is an essential component for survival of a living being. Though the surface of the earth is mostly consisting of water, only a small part of it is usable, which makes this resource limited as well as water bodies are being poorer now-a-days. The impurities, metals in excess amount, chemicals present in water, low pH of water are hazardous to health of a living being. This work focuses on the better techniques for the reduction of impurities, insoluble substances, alkalinity, Total Dissolved Substances and hardness of the water. As plants are rich in secondary metabolites and are used for treatment of various ailments in the indigenous system of medicine. The seed extract from various plants can be used to remove bacterias and heavy metals. Due to its high surface area, charcoal can be used as a catalyst, a filter, or an adsorbent. The charcoal has been used for its adsorptive properties in reducing the impurities in water. The water sample of Krishna river was taken for the study. The work presents a comparative study of effectiveness of water treated with seed extract and charcoal separately in reducing impurities and the results of these two are then compared with the combined treatment of seed extract and charcoal after particular time period. Key words: Water purification, Seed extract, Charcoal

PO – 21 Development of multigrain sweet potato pocket samosa

Abhishek R. Ranvare, Harshad R. Nikam, Abhishek P. Pujari, Pandurang Dure* Department of Food Processing and Packaging, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Samosa is the traditional snack which is originated in Middle East and Central Asia. The main objective of ‘Multigrain Sweet Potato Pocket Samosa’ is to make the samosa healthier and nutritious. Sweet potato is used as replacement for Potato. The major ingredients of Multigrain Pocket Samosa are sweet potato, peas, wheat flour, rice flour, ragi flour, Bengal gram. These all ingredients are collected from Satara local market. Different combinations of four prepared for shell or outer covering of samosa. The sensory analysis of multigrain samosa was done by using 9- point hedonic scale. The proximate analysis of most acceptable was carried out.

PO – 22 Aeluropus lagopoides and Suaeda nudiflora potential halophyte species for phytoremediation

Pawar Uday S.1*, Joshi Arvind J.2 1Department of Botany, A. S. S. M. Mahavidyalaya, Medha, Pin – 415 012, Tal. – Jawali, Dist. – Satara, Maharashtra, India 2Department of Life Sciences, Maharaja Krishnakumarsinhji Bhavnagar University, Gujrat, India *Corresponding Author E-mail: [email protected]

ABSTRACT The contamination of the environment with toxic metals has become a worldwide problem. Metal toxicity affects crop yields, soil biomass and fertility. Soils polluted with heavy metals pose a serious health hazard to humans as well as plants and animals, and often requires soil remediation practices. Halophytic plants are of special interest because these plants are naturally present in environments characterized by an excess of toxic ions, mainly sodium and chloride. Several studies have revealed that these plants may also tolerate other stresses including heavy metals based on the findings that tolerance to salt and to heavy metals may, at least partly, rely on common physiological mechanisms. In addition, it has been shown that salt-tolerant plants may also be able to accumulate metals. Therefore, halophytes have been suggested to be naturally better adapted to cope with environmental stresses, including heavy metals compared to salt-sensitive crop plants commonly chosen for phytoextraction purposes. Thus, potentially the Aeluropus lagopoides and Suaeda nudiflora are ideal candidates for phytoextraction or phytostabilization of heavy metal polluted soils and moreover of heavy metal polluted soils affected by salinity. Integrated findings of present investigation indicated a salt tolerant grass A. lagopoides and succulent halophyte S. nudiflora accumulated maximum amount of Fe in its leaves than any other species. Primary data of the same species occurring at two or more locations showed differences in concentration of heavy metals in its phylloclades or leaves. Accumulation of heavy metals in eight of twelve sampled species was found as: Fe > Mn > Ni > Zn > Cu. Key words: Halophyte species, heavy metals, phytoremediation

PO – 23 Phytochemical analysis from rhizome of Zingiber zerumbet Smith.

Shreedhar S. Savekar*, Yogesh R. Chavan Department of Botany, Yashvantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Zingiber zerumbet smith commonly known as bitter ginger. It is perennial herb native to tropical Asia and Australia. The rhizome of Z. zerumbet has been used as food flavoring and rhizome extract have been used in herbal medicine. Z. zerumbet also known as the "shampoo ginger" because the inflorescence of plant contains viscous juice. Like other plants the Zingiber zerumbet contains various secondary metabolites. The main objective of the project is to evaluate the photochemical present within the rhizome of Z. zerumbet by using qualitative and quantitative analysis of methanol and aqueous extracts with the help of standard techniques. The qualitative test of the rhizome shows presence of carbohydrates, tannin, alkaloids, flavonoids and phenol. Key words: Zingiber, Rhizome, Phytochemical

PO – 24 Sustainable methods for synthesis of indole derivatives

Monali Khatal*, Aboli Sapkal, Suraj Aattar, Santosh Kamble Department of Chemistry, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT various biologically active heterocyclic compounds due to its different application in pharmaceutical chemistry such as anticancer, anti-viral, anti-psychotic, antihypertensive, anti-migraine, anti-arthritis and analgesic activities. Due to such wide application of indole in pharmacy various methodology used for preparation of indole such as synthetic as well as green methodology. During this environmentally conscious day green methodology are more favorable due to its green advantages such as high yields, short reaction time, and inexpensive reagents, highly efficient and environmentally benign over synthetic methods. These green methodologies are microwave, ionic liquids, aqueous, ultrasound, nanocatalyst, green catalyst, and solvent-free reactions etc. Key words: Indole, Ultra sonication, Solvent free, MW, Catalyst free, Aq. medium

PO – 25 Collection, conservation of non-basmati aromatic rice landraces and their use for sustainable agriculture

S. P. Desai1*, A. G. Jadhav1, Y. A. Phonde2, S. G. Ghane1, N. B. Gaikwad1 1Department of Botany, Shivaji University, Kolhapur, Maharashtra, India 2PachimGhat Agro-Forest Producer Co. Ltd. Shahuwadi, Kolhapur, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT India has a rich diversity of rice varieties, and also well known for a wide diversity of aromatic rice landraces. Endemic rice landraces are reported in Western Ghat, consist great diversity regarding scented grains, yield, height, drought and salt resistance. From Kolhapur district, we have collected total of 9 non-basmati aromatic rice landraces like Ajara Ghansal, Ambemohar, Champakali, Jondhala Jirga, Kala Jirga, Kali Kumud, Kothamirsal, Raktisal and Satesali. From all these landraces, we have estimated beta-carotene, macro and microelements and also morphological studies (Kernel length, size and shape) of grains were carried out. The highest amount of beta carotene was estimated in Raktisal (7.05 ± 0.20 µg/g of dry weight) rice landrace while lowest in Ajara Ghansal (1.84 ± 0.12 µg/g of dry weight). Nitrogen and Potassium were estimated in higher amount in Ajara Ghansal i.e., 1.82 % and 1.10% respectively. Sodium, Calcium and Sulphur were estimated in a higher amount in Champakali landrace. Magnesium was estimated in a higher amount in Kothamirsal landrace (11.47%). Boron and Ferrous were estimated in a higher amount in Jondhala Jirga landrace i.e., 27.46 PPM and 553.36 PPM respectively. In Kothamirsal (473.74 PPM), Kala Jirga (388.90 PPM) and Ajara Ghansal (434.25 PPM) landraces a good amount of ferrous reported. Zinc and Copper were estimated in higher amounts in the Kothamirsal landrace i.e., 57.94 PPM and 31.19 PPM respectively. Maximum kernel length was recorded in Raktisal while minimum in Jondhala Jirga. The grain quality was super fine and medium and the shape was slender, medium and bold. For conservation Strategies,

Department of Botany, Department of Zoology, Department of Microbiology, Department of Biotechnology and Department of Food Processing and Packaging of Yashavantrao Chavan Institute of Science (Autonomous College), Satara – 415 001, Maharashtra, INDIA 59 International E-Conference on “Current Approaches in Life Sciences for Sustainable March 19 & 20, Development” (ICCALSSD-2021) 2021 we have distributed seeds of landraces to rice-growing farmers in Kolhapur and its adjoining area. Farmers are so happy because they have received their old rice seeds with them and we have created awareness about rice landraces conservation and its use in sustainable agriculture. Key words: Aromatic, Conservation, Landraces, Sustainable agriculture and Western Ghat

PO – 26 The study of pre-treatments effect on fishy flavor and nutritional value of fish powder

Misal Saraswati Dattu1*, Jadhav M. H.2 1Department of Food Science and Technology, Shivaji University, Kolhapur, Maharashtra, India 2Department of Food Processing and Packaging, Yashavantrao Chavan Institute of Science, Satara, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Fish is one of the protein rich food and is excellent source of the essential fatty acids. Most of the people avoid fish meal due to its intense fishy odor. Fish species gives foul smell which comes from the compound trimethylamine oxide. TMA is the molecule responsible for the fishy odor associated with seafood. For the removal of TMA, five different pre – treatments were tried. Different concentrations of kokum, ginger, vinegar, casein, and combination of kokum and ginger solution were applied on thawed fish for stated time period. Followed by steaming up to desired texture. The drying of fish pieces was done by using a mechanical dryer (tray dryer) at constant air flow rate and constant temperature with varying thicknesses up to desired moisture content. After drying the dried products were ground to make powder. Sensory analysis of fish powder was carried out to determine reduction of fishy odor. Sensory analysis was done with 9-point hedonic rating scale by 10 semi trained panel members. Proximate analysiss of fish powder was done with AOAC methods. The treated fish powder that gives maximum reduction of fishy odor and provide better nutritional value such as 0.13% moisture, content 9.5% fat, 81.36% protein, 0.46% ash and 0.14% moisture, 10.5% fat, 85.82% protein, 0.47% ash by ginger and kokum respectively. In all above pre – treatments, kokum and ginger were effective at reducing the fishy odor resulting from the trimethylamine in fish powder. Fish powder can be used as base for preparing many other protein rich products. A great use of fish powder can be achieved for texturising formulation due to being odourless.

PO – 27 Development and sensory evolution of Oats and puffed rice chocolate

Ranvare Abhishek Rajendra, Harshad R. Nikam, Abhishek S. Pujari, Pandurang Dure* Department of Food Processing and Packaging, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT The Development and Sensory evolution of Oats and puffed rice chocolate was carried out. Chocolates are helpful to control the hunger. The main objective to develop the oats and puffed rice chocolate is to control the hunger. Oats and Amaranth helps to increase the dietary fiber level in final product. Amaranth gives smooth texture to the final product. In these chocolate dates are used in double quantity to bind all ingredients. Oats and puffed rice chocolate give 417.Kcal per 100g. Dark chocolate is used to coat molded mixture of oats, Amaranth, Dates and dark chocolate.

PO – 28 Study of diseases and pests of Ginger in Satara district

P. S. Rasal*, B. A. Kore Department of Botany, Yashvantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Zingiber officinale Roscoe. from family Zingiberaceae is commonly known as ginger. Ginger is an important spice crop in India which is also leading producer and exporter of ginger in the world. During cultivation, ginger is affected by many diseases and pests. In Satara district ginger is cultivated as a major crop in areas like Rahimatpur, Koregaon, Nagthane, Parali where crop is infected by various diseases like soft rot, bacterial wilt, leaf spot and insect pests like rhizome fly, white grub, shoot borer and leaf roller that causes serious economical loss in the yield. In present study we discussed about symptoms, causal organism, disease cycle and methods of management of above-mentioned diseases and pests. Key words: Ginger, diseases, pests, management

PO – 29 Proposing application of 3D scanning technology and machine learning in crime scene documentation and autopsy

Anantkumar R. Patil, Samidha S. Walvekar, Manjushri S. Bagul Department of Forensic Science, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected] [email protected]

ABSTRACT A forensic autopsy is a series of tests and examinations performed on the body to determine the presence of an injury and/or to identify any disease that may have caused or contributed to the death. 3D laser scanning is a way to capture a physical object’s exact size and shape into the computer world as a digital 3-dimensional representation. 3D scanning and modelling will provide extreme realism and ease to reconstruct the crime scene. By scanning and modelling of the body we can preserve the external findings of the body and access them as per the need of the investigator as well as to present it to the court of law. And to protect the data from tempering we will secure it with hash value, hash functions so that integrity of the evidence is maintained. In Our project we are implementing 3D scanning Artificial intelligence and machine learning for crime scene sketching and forensic autopsy. We conduct the process in 3 Phases. In 1St phase; we will scan different simulated crime scenes. Then we will work with the artificial intelligence and machine learning part in second phase. And in phase 3 we will scan bodies during autopsy to find how useful the scan models can be for investigation processes. In this project we have received very detailed scan models as outcomes in scanning of crime scene. Key words: 3D scanning, Artificial Intelligence, Machine learning, Forensic autopsy, Tempering

PO – 30 Green synthesis of Copper nanoparticles from plant extracts & its antimicrobial activity

Sneha Chatterjee, Vaibhavi Gulwani, Pratibha Patil* Department of Applied Microbiology, Yashvantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Green chemistry is a rapid emerging field of chemistry, its aim is to reduce the effect of damage caused to the environment by manmade materials and the processes used to produce them. In the current study, we have carried out green synthesis of copper nanoparticles using leaf extracts of Psidium guajava, Annona reticulata, Eucalyptus. In this, we report the synthesis of copper nanoparticles using copper acetate as precursor and leaf extract as reducing agent. The leaf extract was added to 0.5% copper acetate solution and observed the change in colour. Further, this has to be characterized by FT-IR and XRD. Key words: Green synthesis, Psidium guajava, Annona reticulata, Eucalyptus, copper nanoparticles

PO – 31 Microbial production of melanin from farm yard compost

Avadhut Patil, Pradhumn Shitole, Rishikesh Rajput, Pratibha Patil* Department of Applied Microbiology, Yashvantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Melanin are the natural pigments which have their presence in animals, plants and in most of the microorganisms. The current study is aimed to isolate and identify melanin producing bacteria. The farm yard compost was randomly collected from district Satara. A nutrient agar medium containing tyrosine was used for isolation of melanin producing bacteria and then pure culture in nutrient broth containing L- tyrosine which were examined for biochemical and morphological test. Isolates were then subculture on nutrient agar medium containing tyrosine for production of gas from glucose, growth in presence of NaCl, Pᴴ tolerance, growth temperature and enzymatic activity. The melanin pigment was further characterized by FT-IR spectroscopy. The FT-IR result confirms that it was melanin pigment. Therefore, this study proved that compost sample can be used for the production of melanin and it has resistant anti-bacterial activity. In present study different bacterial species and fungal species were identified. Key words: Melanin producing bacteria, melanin pigment

PO – 32 Isolation of antibiotic producing Endophytes in cactus Opuntia humifusa

Bhosale P. C, Wadikar S. S* Department of Applied Microbiology, Yashvantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Opuntia humifusa is an invasive plant well established in the harsh western arid zone of Maharashtra state, India. Evidence suggests it is likely that the endophytic fungal populations of Opuntia humifusa assist the host in overcoming biotic and abiotic stress by producing biologically active metabolites. With this in mind there is potential to discover novel natural products with useful biological activities from this poorly investigated source. Consequently, an investigation of the antimicrobial, antibiotic producing activities of the endophytes of Opuntia humifusa, that occupies Opuntia humifusa, a unique ecological niche, may well provide useful leads in the discovery of new pharmaceuticals. Key words: Opuntia humifusa, Endophytes, Antibiotic, Active metabolites

PO – 33 Collection, conservation, beta carotene and mineral analysis studies of non aromatic rice landraces from Kolhapur district of Maharashtra

A. G. Jadhav1*, S. P. Desai1, S. G. Ghane1, Y. A. Phonde2, N. B. Gaikwad1 1Department of Botany, Shivaji University, Kolhapur – 416 004, Maharashtra, India 2PachimGhat Agro-Forest Producer Co. Ltd. Shahuwadi, Kolhapur, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Rice (Oryza sativa L.) is important staple food as well as cereal crops which consumed by 2/3 population of the world. From Kolhapur district, we have collected 40 non aromatic rice landraces during 2016-2021. Seed powder of all the rice landraces were used for beta carotene analysis and mineral analysis. Beta carotene content ranges from 0.899 to 5.879 µg/g of dry weight. Highest β-carotene content was observed from Masad 5.873 µg/g rice landrace. In case of macro and micro elements Nitrate, Moisture, Total ash, Protein content analysis was carried out from 40 non aromatic rice landraces, it was observed that Sawashin, Takabhat, Lavhi Airath, Bhogawati, Tulshibhat, Walai, Tamsal, Mothiratna, Dandeli, Dodga, Bhadas, Sorti, landraces are showing promising reports and required conservation of these landrace. Similarly, these landraces can be used for future breeding program so as to incorporate the nutritional values in improved cultivars or varieties and also can be used for nutritional purpose. Key words: Non aromatic rice landraces

PO – 34 Use of heterogeneous catalyst in organic transformation

Avdhut D. Kadam*, Ankita Phadatare, Rutuja Pawar, Santosh B. Kamble* P.G. Department of Chemistry, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected] [email protected]

ABSTRACT The develop nanoparticle catalyst protocol is beneficial because of recyclability of catalyst, high yield, shorter reaction time, easy workup. The Fe3O4 nanoparticles were synthesized by using co-precipitation method from FeCl3.6H2O, FeCl2.4H2O, and precipitating agent like NH4OH. Catalytic activity of nanoparticles is obtained by synthesis of amine (aniline) by using nitrobenzene and hydrazine hydrate in methanol as a solvent. Fe3O4 as an efficient catalytic system for nitroarene reduction. Magnetically separable catalysts are very attractive heterogeneous catalysts owing to their facile recycling process. Among them, magnetic nanoparticle catalysts have attracted much attention because they show good dispersibility and allow easy recovery after the reaction. Herein, we report the utilization of commercially available Fe3O4 nanoparticles towards efficient and selective nitro group reduction.

Key words: Fe3O4 nanoparticle, heterogeneous catalyst

PO – 35 Biodegradation of oil sludge waste by using fungi

Patil V. S., Bhosale S. L., Shete S. S., Sawant P. S., Patil P. S.* Department of Applied Microbiology, Yashvantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Oil sludge is thick viscous mixture of sediments, water, oil and hydrocarbons. Polycyclic aromatic hydrocarbons which they are components of oil sludge and it constitute serious environmental effects as many of them are cytotoxic, mutagenic and also potentially carcinogenic. Improper management and disposal of oil sludge causes the ecosystem and create environmental pollution. The sample was collected from oil contaminated soil. The sample was analyzed for oil sludge degrading fungi by using sabouroud's medium. The degradation of oil sludge was observed by using the sabouroud's broth culture of the fungi for a period of 7 days. The fungi strain showed high activity in oil sludge waste degradation with 4 days. The analysis of oil sludge degradation by using fungi in sabouroud's broth medium was observed, fungi degraded the oil waste component in sabourds broth and it was compared with control. In present work, oil sludge degradation was studied by using fungi. The color changed in sabouroud's broth medium indicate high ability of oil sludge degradation. Key words: degradation, oil sludge, environmental effects

PO – 36 Biosynthesis of Copper nanoparticles by an isolated fungus

Pradnya B. Zambare1,2, Shivangi Shivraj Kanase2* 1Rayat Institute of Research and Development (RIRD), Satara – 415 001, Maharashtra, India 2Department of Microbiology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Biosynthesis of metal oxide nanoparticle using micro-organisms is an important area of research in nanobiotechnology. Biological method of nanoparticle synthesis is cost effective, ecofriendly method as compared to chemical and physical method. The present study deals with extracellular production of copper nanoparticles using Aspergillus. The synthesized CuNPs have been characterized by UV-Visible spectroscopy, FTIR and XRD. The visual observation shows colour change from colourless to light green when extract from fungus contacted with copper sulphate as a precursor indicating formation of copper nanoparticles. UV-Vis spectroscopy showed maximum absorbance at 350 nm. FTIR analysis performed to identify biomolecules and capping reagents of fungal extract that may be responsible for reduction of copper ions and stabilization of copper nanoparticles. The X-ray diffraction (XRD) pattern was used to analyze the phase and crystal structure of biosynthesized nanoparticles. XRD studies showed peaks at 43.20, 50.320, 73.990 .The high intensity and broaden diffraction peaks evidently states that synthesized CuNP are highly crystalline in nature. Key words: biosynthesis, Copper nanoparticle, nanobiotechnology, ecofriendly

PO – 37 Antibacterial properties of elicitor treated callus cultures of Salacia chinensis L.

Avdhoot Patil, Akshay Deshmukh, Pallavi Panari, Sarika Chavan, Jaykumar Chavan* Department of Botany, Yashavantrao Chavan Institute of Science (Autonomous), Satara – 415001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Saptarangi (Salacia chinensis L.) is a chief source of several bioactive compounds that have many advantageous effects on human health including antidiabetic, anticancer and anti-HIV. The present study aimed to investigate the antibacterial potential of callus following application of different elicitor treatments for S. chinensis. The leaf-derived callus cultures were established on Murashige and Skoog’s (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D: 2.0 mg/l) and 6-benzylaminopurine (BAP: 1.5 mg/l). These cultures were treated with different elicitor’s viz. jasmonic acid (JA), methyl jasmonate (MeJA) and yeast extracts (YE). Elicitated calli of S. chinensis demonstrated the promising antibacterial potential against seven laboratory pathogens viz. Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris and Salmonella typhi. The callus obtained on MS medium supplemented with 2,4-D (2.0 mg/l), BAP (1.5 mg/l) and treated with JA (75 µl) displayed augmented values for minimum inhibitory concentration against P. vulgaris as compared to control. Key words: Antibacterial, Callus, Elicitation, Salacia chinensis

PO – 38 lnstant multigrain chicken curry mix

Mayuri Mohan Pawar, Nilam Sanjay Bhosale Department of Food Science and Technology, Shivaji University, Kolhapur - 416 004, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Chicken is one of the highly nutritious food. It is perishable in nature, that's why we need to convert it into dry form. chicken is the richest source of protein but the carbohydrate content is low. Various cereals and legumes were used for the preparation of curry. The cereals and legumes are the excellent sources of protein and fat and also plays an important role in reducing the flavour of chicken. To prepare the instant multigrain chicken curry mix, the cereals and legumes was soaked in water for overnight to reduce the cooking time. Then, hydrated cereals and legumes with chicken was cooked for desirable time and smashed. After smashing, the slurry was shallow fried with paste (ginger, garlic and onion) by using ghee. The prepared puree was dried in tray dryer as per favorable temperature and time. Then the dried material was ground into fine powder. After that dried onion, capsicum, carrot and spice mix was added into powder. The Instant multigrain chicken curry mix was prepared. The sensory analysis of instant multigrain chicken curry mix was carried out to evaluate the sensorial properties. The sensory analysis was done with 9-point hedonic rating test by 10 semitrained panel members. The proximate analysis was done with AOAC method. Instant multigrain chicken curry mix provides 330.72 kcal energy, 38.8% protein, 18.14% fat, 2.4% carbohydrate. Microbial analysis was done with standard plate count. The Shelf life of the final product was calculated up to 3 months by microbial analysis. Instant multigrain chicken curry mix can serve for time saving and convenience. This product can be a healthy and great nutritional alternative with easy making process. And also, it's good for old age people.

PO – 39 Antimicrobial activity of plant leaf extracts against plant pathogenic microorganisms

Rutuja Zanjurne, Devyani Pawar, Aishwarya Khutale, Pratibha Patil* Department of Applied Microbiology, Yashvantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Antimicrobial activity refers to a potential of killing the disease-causing microbes. Plant produces some bioactive biomolecules which showed antimicrobial activity against pathogens. Following this, we have carried out experiments in which we run the sets of pure leaf extracts and solvent extracts of Ricinus communis (Castor), Millettia pinnata (Karanj), Azadirachta indica (Neem) and Annona reticulata (Custard apple) where out of this these four plant leaves extracts only Ricinus communis and Millettia pinnata showed maximum zone of inhibition against plant pathogen like Xanthomonas and Aspergillus. In further study we would isolate and characterize the bioactive compounds of plant which are responsible for pest control. Key words: Antimicrobial activity, Plant extract, Bioactive compound, Plant pathogen

PO – 40 Isolation and partial purification of L- glutaminase from halophilic bacteria

S. H. Nadaf 1, P. B. Dandage1*, N. H. Nadaf2 1Department of Biochemistry, Vidya Nagar Shivaji University, Kolhapur – 416 004, Maharashtra, India 2Department of Microbiology, Vidya Nagar Shivaji University, Kolhapur – 416 004, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT L-Glutaminase enzyme was used mostly in therapeutic agent because it has anticancer, antiviral and other pharmacological activities. The attempt will be taken to isolate a glutaminase producing halophillic microorganism from saline soil of shirol Taluka, Kolhapur district. Isolation of organism was done by using enrichment of soil sample followed by the serial dilution and spreading technique. For isolation of microorganism minimal agar supplemented with 0.1 % L- glutamine and 1% salt concentration was used. L - Glutaminase is extracellular enzyme which converts glutamine to glutamic acid and ammonia and the isolates are identified by colony showing colour change of the media. Out of the 30 isolated halophilic and halotolerant bacterial strains, 4 potent strains of lglutaminase producers were selected. Enzyme activity is observed by using semi quantitative way by using solid media and quantitative assay by using Nessler’s reagent which detects the amount of ammonia produced. Partial purification of enzyme was carried out by using ammonium sulphate precipitation method. This partially purified enzyme can tolerate maximum 1% NaCl concentration. The optimum pH is 7 and optimum temperature is 37 0C. Key words: L-glutaminase, glutamine extracellular enzyme, halophilic bacteria

PO – 41 A Microbial Approach: A sustainable way for plastic degradation

Akshata S. Phalke, Pramod V. Chavan* Department of Chemistry, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT All over the world since last 100 years wooden materials, materials made by glass, metals have been replaced by plastic polymers. The distinct properties such as strong, non-reactive make it desirable for daily use, but its non-biodegradable property increase in plastic waste which creates a global environmental problem that is yet to be resolved by researchers and related stakeholders. Plastic waste deleteriously affected life on earth, living beings, aquatic ecosystem. The current methods for eliminating these wastes are unsustainable, come at massive costs and put more burden on our environment. Thus, recent focus has been placed more on the potential of biological systems to degrade synthetic plastics. In this regard Biodegradation of plastic by microorganisms is a sustainable way. Hence, in the light of recent literature, this review emphasizes the multifaceted roles played by microorganisms in this process. Key words: Plastic degradation, microorganism, sustainability

PO – 42 Preparation of herbal formulation from leaves of Oscimum americanum & Eclipta alba and it's efficiency against the fungal diseases and wounds in dairy animals

Tuwar D. A.1*, Satre T. S.2, Tuwar A. R.3 1Department of Botany, Arts, Commerce and Science College, Sonai. Tal- Newasa, Dist - A. Nagar, Maharashtra, India 2Department of Botany, Dahiwadi College, Dahiwadi, Tal. Man, Dist. Satara, Maharashtra, India 3Department of Botany, Arts, Commerce and Science College, Sonai, Tal. Newasa, Dist - A. Nagar, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT In present study organic formulation was prepared by using two plants for controlling fungal diseases as well as wounds i.e., Oscimum americanum (Rantulas) &Eclipta alba (Kalamaka). The main constituents are Eugenol, Methyl Chavicol, Terpineol, Linalool in O. americanum (Rantulas) and Stigmasterol, Wedelolactone, Desmethywedelolactone in E. alba (kalamaka). These chemicals have different activities like antifungal, antibacterial, insecticidal, and anti-viral and also shows wound healing properties. Powder was made & homogenize in 80 % Methanol and keep this mixture for 24 hours and filter through Whatman filter paper. Evaporate this extract in water bath to obtain semi solid extract and simultaneously to collect methanol for reuse. Semi solid extract is directly use to prepare formulation with use other chemicals. The testing of formulation on fungal disease and wound healing were carried out on dairy animals like Cow & Buffalo. The testing showed that fungal disease and wound healing were cure. The period for cure disease and wound is different in different proportion i.e., 8-16 days. Therefore, this formulation first might be a potential source for the treatment of fungal diseases and wound healing in animals and it more efficient than available ointments. Key words: Rantulas, Kalamaka, fungal diseases

PO – 43 Effects of pharmaceutical (Diclofenac) in fresh water fish Garra mullya

Anjali T. Gujar*, Shradha A. Kshirsagar, V. Y. Deshpande Department of Zoology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Medicines and drugs are used to cure diseases in animals and humans but some medicines are now a days responsible for aquatic pollution. Diclofenac as pollutant has been studied by many workers. 1) Adhikari, S., Sarkar, B., Chattujee, A., Mahapatra, C. T. and Ayyappan, S. 2004. The present study was carried out to study the effect of diclofenac on fish Garra mullya to determine toxicity and lethal effect on liver. For toxicity 96 hrs static bioassay exposure shows LC0 value of 0.32 mg/lit and Lc50 concentration was 0.36 mg/lit. The histological changes were observed in selected organ liver as compared to control.

PO – 44 Formulation and sensory evolution of ready-to-cook multigrain chicken meat balls

Kiran Hiralal Pawar Department of Food Processing and Packaging, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT The demand of low-fat chicken is growing within the population. Meat products are rich source for their protein source. The skin of chicken is high in fat, it can increase the fat level in final product. So, the skin of chicken should remove before cooking. Chicken breast is low in fat and easy to cook. Meat balls are the famous products in the India. There are several types of meat balls like beef, fish, prawn and chicken meat balls. Normally bread crumps are used as binder in chicken meat balls. In multigrain chicken meat balls Millets are used as functional binders. Ragi millet and pearl millet are used as functional binders which help to increase the sensory acceptability of meat products. Oats are incorporated in meat balls to increase the dietary fiber level of multigrain meat balls.

PO – 45 Study of physicochemical and microbial analysis of soil from Kaas plateau

Shinde Shraddha, Kulkarni Asmita, Salunkhe Mayur, Kumbhar Kailas* Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Kaas plateau has unique characteristics which holds different ecological systems. Many microorganism and endemic, endangered plants and animal. In the present study, soil sample from kaas plateau was collected during the rainy season. After collection of soil sample screening techniques were performed. Ten isolates were found. Among the 10 isolate 7 are gram positive and 3 are gram negative. For detection of unknown organism several biochemical tests were performed. This biochemical test result was compared with Bergey’s Manual for further study of biological activity. The soil sample also analysed by using physicochemical test as pH, nitrogen, potassium and phosphorous content. Also, the chloride and electrical conductivity of the water collected from kaas soil were analysed. Key words: Kaas soil, biochemical test, physicochemical test

PO – 46 Effects of dietary inclusion of synbiotics on protein content, enzyme activity and economic parameters in Bombyx mori L.

Nalavade Virdhaval Madhav1*, Nalawade Savita Pravin2 1Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India 2Department of Zoology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Nutrition has a significant importance in improving the growth and development of silkworm Bombyx mori. Silk yield is mainly dependent on healthy disease-free larval growth and higher nutritive value of mulberry leaves. Previous studies showed use of synbiotics had higher economic benefits and reduced mortality in silkworm. The present investigation was carried with an objective to determine the impact of fortification of V1 mulberry leaves with Serigrow++ (containing Spirulina, yeast extract and probiotic bacteria of Lactobacillus Spp.) at 1000 ppm on protein content, growth and commercial traits of B. mori (CSR2 × CSR4). Feeding of fortified leaves with Serigrow++ (1000 ppm) led to increase in 52.73 % protein and 49.7% larval weight over control fifth instar larvae of CSR2 × CSR4 silkworm hybrid. The enzyme activity for protease and amylase was significantly higher in III, IV and V instar in all treated samples in comparison to control samples. Highest protease and amylase activity was observed in Vth instar larva, which was 37.84% and 35.15 % increase over control respectively. Significant increase in various economic parameters like coccon weight, pupal weight, shell weight and silk filament for treated samples over control were observed. Hence, the results of present study clearly designate the effectiveness of Serigrow++ showed better growth, rearing performance, development of silkworm larvae, quality and quantity of silk in B. mori. Key words: Bombyx mori, Serigrow++, synbiotics, probiotics, prebiotics, protein content

PO – 47 Antioxidant, cytotoxicity and quantum chemical descriptor study of several coumarin derivatives”: computational analysis

Sharad G. Jadhav1*, Vishakha Deshmukh, Aishwarya patil Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT The series of nine coumarin derivatives (CDs) were evaluated for in-vitro antioxidant activities through total antioxidant capacity of 1, 1-diphenyl-2-picryl- hydrazyl (DPPH), hydroxyl radical and superoxide radical scavenging activity. Cytotoxicity studies were carried out against cancer cell lines (MDA-MB-231 and HEP-3B-PN-11) by performing (4,5-dimethylthizol-2yl)-2,5-diphenyltetrazolium bromide-based MTT assay. The physico-chemical properties of the test CDs were calculated using BioMed Cache 6.1.10. Experimental and in-silico results of the present investigation shown significant cytotoxicity against cancer cell line in an

IC50 range of 8.31- 14.65 µM respectively. While, all the selected CDs demonstrated considerable dose dependent DPPH, SOR and OH radical scavenging activity. The obtained results highlight the potential of selected CDs as significant antioxidant and anticancer agents. Key words: Coumarin, Antioxidant, Cytotoxicity, Computational analysis

PO – 48 Applications of melanin associated silver nanoparticles for photocatalysis of malachite green dye and it’s phytotoxicity analysis

Swati T. Gurme*, Suchita V Jadhav Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Melanin, an indolic polymer widely distributed throughout the living world former the evolution of life. Melanin is distributed in the living forms and has diverse functional attributes, mainly protection of the organism. Melanin has been reported by different microorganisms earlier and has versatile applications in different fields. In present study synthesis of silver nanoparticles using melanin as a reducing agent was carried out along with the characterization of synthesized nanoparticles. In order to examine the photocatalytic activity of prepared melanin associated AgNPs, the malachite green (MG) dye was used as a model contaminant. With the increase in dye concentration, i.e., 10 to 50 PPM, the % degradation of MG was decreased from 73.22% to 37.52% and a further increase in the concentration of dye to 250 PPM, the % degradation was dropped to 20.15% at 30 min. The time- dependent percent degradation of brilliant green at high concentration of 250 ppm was recorded using UV–visible spectrophotometer. Toxicity of degraded MG was accessed by performing phytotoxicity analysis and confirmed the usability of melanin associated AgNPs in the cosmetic and pharmaceutical industries as well as in textile industries as they have superior photocatalytic activity. Key words: Melanin; Silver nanoparticles; Malachite green; Phytotoxicity

PO – 49 Microbial and Biochemical studies on gut bacteria isolated from fresh water fish Garra mullya

U. L. Shevale1,2*, Jadhav P. P.1, S. M. Pawar2 1Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India 2Department of Zoology, Lalbahadur Shastri College of Art, Science and Commerce, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Probiotics represents probably the archetypal functinal food and are defined as activ microbial supplements which beneficially affect the host by improving its intestinal microbial balance. The gut microbiota of fresh water fish Garramullya was studied to evaluate the functional propertiesof probiotics like acid tolerance, bile salt tolerance, antagonistic activity, antioxidant activity, antibiotic susceptibility and qauntitative estimation of enzymes. Total 5 isolates were studied for present investigation. These bacterial isolates were procured from Department of Biotechnology, YCIS, Satara (Ms), India. The bacterial isolates were tentatively identified as Bacillus substilis (A6), Bacillus amyloliquefaciens (A7), Psedumonas species (A8), Bacillus polmyxa (A9), Peudomonas (A10). Among 5 isolates A7 and A9 were shows excellent and A6, A8 and A10 shows moderate probiotic properties including ability to survive and grow from pH range 2 to 7, tolerate bile salts, show high antimicrobial activity against selected human pathogens such as Escherichia coli and Staphylococcus aureus. In addition to this, these isolates were shows antibiotic susceptibility to several antibiotics, and also capable of showing antioxidant activity. These isolates are also showed to produce amylase enzyme quantitatively. The amount of enzyme produced by these isolates were calculated by Lowry Method. The properties shown by these isolates may indicate that they could colonize and persist in the G.I.T. as well as reduce the risk of drug resistant microorganisms and hence induce beneficial effects to the host and total aquatic ecosystem. The present investigation may be useful tool to develop probiotics to improve animal health and reduce the risk of gastrointestinal disorders in aquaculture industry. Key words: Probiotic, Amylase, Garramullya, Antagonistic activity, Antioxidant

PO – 50 Development of instant fish soup mix

Amruta J. Ranaware*, Rasika R. Mane, Pavani V. Gadpurpwar, Shital A. Patil, A. K. Sahoo Department of Food Technology, Shivaji University, Kolhapur – 416 004, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT The present study was conducted to optimize the process for the development of an instant fish soup mix. The fish was washed with 1% brine solution and cooked at 100oC for 10 min with 2% vinegar solution. The vegetables such as onion, carrot, celery was balanced at 90°C for 5mins followed by dicing and drying using a cabinet dryer at 60°C up to desired moisture content. Later, all ingredients were varied in composition in different samples, and sample B was selected based on a sensorial evaluation, received the highest score by semi-trained panel members. The readied Physico-chemical, sensorial and microbial properties of sample B were stable for up to three months. Hence the study revealed that dehydrated fish, onion, carrot, celerey mixed with a proper amount of spice powder along with MSG, CMC, and ascorbic acid gives a nutritious and palatably accepted product. Key words: King Mackerel Fish Powder, Vegetables powder, Spice powder, Monosodium glutamate

PO – 51 Extraction and characterization of collagen from scales of Catla catla

Sanchita Pandit, Balaji Kanwate* Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Collagen is widely used in the food and pharmaceutical industries due to its nutritive values and functional properties. Fish processing by-products such as skin, scale, bones, head, viscera and swim bladder were considered as rich sources of collagen and gelatin extraction. Against this background, the present work aimed to study the extraction of collagens from scales of Labeo catla (Catla fish) using acid and alkali pre-treatment and subjected to different physicochemical and functional properties. The yield and proximate composition were determined. Fourier transform infrared spectra (FTIR) of collagen from catla fish scale is observed and suggesting the presence of collagen. The solubility of collagen at different pH (1-10) was investigated Therefore, collagen can be extracted with superior properties from catla scale using acid and alkali pre-treatment. Furthermore, the extracted collagen could be used in the food and pharmaceutical sector. Key words: Catla, scale, collagen, proximate analysis, FTIR, solubility

PO – 52 Production and characterization of tyrosinase activity by microbial source isolated from meat waste

Yasmin A. Mulani, Kailas B. Kumbhar* Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Tyrosinase is the natural enzymes that can be obtained from the multiple sources like bacteria, fungi, plants, mammals and can only purified to a very low degree. The isolated microorganism from meat waste were evaluated for Tyrosinase enzyme activity using low-cost media. The tyrosinase enzyme produce by four isolated culture which is extracted and activity was check by using pH, Temperature, biochemical test, Tyrosine assay, polyphenol oxidase activity, Ammonium sulphate precipitaion etc. All selected isolate demonstrates considerable enzyme activity the obtained result highlights the potential of tyrosine enzyme. Key words: Tyrosinase, Meat waste, Enzyme activity

PO – 53 Evaluation of Araneae diversity on Mangifera indica from Lengare, Maharashtra, India

Misal P. K.1,4*, Bendre N. N.2,4, Pawar P. A.3,4, Bhoite S. H.4 1Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India 2R. B. Narayanrao Borawke College, Shrirampur, Dist. Ahmednagar, Maharashtra, India 3Dada Patil Mahavidyalaya, Karjat, Dist. Ahmednagar, Maharashtra, India 4Drongo Nisarg Sanshodhan, Sanvardhan and Sanrakshan Sanstha, Satara, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Aim of the study was to explore the diversity of Araneae (spiders) on Mangifera indica (Mangotree) which is known as a mini habitat of various spiders. Evaluation was carried out during year 2018-2019. We observed 16 species of 5 different families like Salticidae, Araneidae, Theridiidae, Sparassidae, Thomisidae. Among this, salticidae family was found to be Dominant due to active hunting predatory behavior followed by Araneidae family. This study imparted conservational values of different trees in tropical regions which nurture the diversity. Therefore, we conclude that presence of varied number of spiders that we identified was due to the vast availability of prey on the Mangotree. Thus, Araneae diversity helped acting as biocontrol agent on the mangotree. Key words: Diversity, Araneae, Mangifera indica, Biocontrol

PO – 54 A review on synthesis of zinc oxide nanoparticles using plant extracts and their characterization

S. J. Mane, A. S. Pisal, G. S. Jadhav* Department of Zoology and Fisheries, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Zinc oxide nanoparticles are significant applications in agricultural and Medical field. But excess use of Zinc metal oxide nanoparticles is harmful to environment and human being. Synthesis of nanoparticles are various methods like physical, chemical and biological method. Due to environmental, health, economic and medicinal benefits biological method is an appropriate alternative method for the production of Zinc oxide nanoparticles using plant sources. This review described the summary of the synthesis and characterization technique of Zinc oxide nanoparticles. Key words: Green Synthesis, Zinc oxide, Nanoparticles, Characterization

PO – 55 Efficacy of Withania somnifera in metform in induced aged mice

Sawant M. D.*, Bajabale P. C., Bhosale P. S. Department of Zoology and Fisheries, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Life expansion is the concept of extending the human life span through improvements in medicine. Through out the last several years, an intensive search of anti-aging treatments has led to the discovery of a variety of drugs that encourage life extension. Metformin is sometimes proposed to bean “anti-aging” drug, based on preclinical experiments with lower order organisms and several retrospective data on helpful health out comes for type 2 diabetics. Ashwagandha is an excellent adaptogen that is being used since ancient times in Ayurvedic medicine. Traditionally, it is used for various ailments and general well-being, including the treatment of aged patients. Managing quality of life remains a challenge for the elderly population, especially joint pain management, sleep, and general well-being. The objective of this study was to assess the safety, efficacy of Ashwagandha (Withania somnifera (L.) Dunal.) leaves extract on the improvement of general life spanin induced mice. Key words: Withania somnifera, Metformin, Aging

PO – 56 Occurrence and enumeration of arbuscular mycorrhizal fungi in soybean Glycine max (L.) Merr.

P. D. Sagare, N. B. Mane* Department of Botany, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Arbuscular Mycorrhizal Fungi is used for crucial importance for their efficient use in environmental conservation and sustainable agriculture. Present research work showed occurrence and enumeration of AM Fungi in Glycine max (L.) Merr. We surveyed Rhizospheric soil samples from Tasgaon area of Sangli District. Screening of soil samples depicted ten AM fungal taxa belonging to 3genera based on spore were isolated from Glycine max. The dominant Glomus, Acaulospora and Scutellisopra AM fungi were isolated. Glomus geosporum (Nicol. & Gerd.) C. Walker, Glomus multicaule Gerd. & B.K. Bakshi, Glomus macrocarpum Tul & C. Tul., Glomus constricuctum Trappe, Glomus crenatum, Glomus spp., Acaulospora laevis (Walker & Sanders), Scutellospora spp. Walker & Sanders., Acaulospora scrobiculata Trappe forming predominant species. Maximum population occurred at 90 days plant than vegetative 30 days plant. Key words: Arbuscular Mycorrhizal Fungi, Rhizospheric soil, Glomus, Acaulospora

PO – 57 Effect of seaweed extract on protein profile silkworm of Bombyx mori

Komal Pawar, Yogita Patil, S. P. Nalawade Department of Zoology and Fisheries, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Silkworm Bombyx mori is a domesticated and economically important insect which is primary producer of silk. The important growth regulating factor in silkworm is nutrition. Growth and development of silkworm larvae and cocoon yield depends on dietary nutrients. Enriching mulberry leaves by nutrient supplementation is one of the ways to improve growth rate in B. mori. Study was conducted to evaluate the potential of seaweed extract on growth and protein content of larval instars of B. mori. In present study second to fifth instar larvae were fed with 0.1 %, 0.3 %, 0.6 % concentration of seaweed extract. The larvae fed with mulberry supplemented with seaweed extract shows significant growth and increased protein content in relation to control. The growth and development of larvae, and subsequent cocoons production, are greatly influenced by the nutritional quality of mulberry leaves with seaweed extract. The results indicate that application of seaweed extract significantly increases size, weight, cocoon yield and protein content of the larval instars of B. mori. Key words: Bombyx mori, Silkworm, Mulberry leaves, Seaweed Extract

PO – 58 Differential response of local landraces of finger millet (Eleusine coracana (L.) Gaertn.) to salinity stress in relation to growth

Sumaiya S. Shaikh1, Nitin T. Gore1, Abhinav A. Mali1, Suraj D. Umdale2, Pankaj Mundada3, Mahendra L. Ahire1* 1Department of Botany, Yashavantrao Chavan Institute of Science, Satara 415 001, Maharashtra, India 2Department of Botany, Jaysingpur College, Jaysingpur 416 101, Maharashtra, India 3Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Salt stress affects almost all the stages of plant growth however seed germination and early seedling development are more sensitive and can be considered as crucial for screening of crop genotypes. The effect of sodium chloride (NaCl) induced salt stress was studied in 26 local landraces of finger millet under various levels (0 – 250 mM) of salinity. NaCl stress resulted in decreased germination percentage, shoot length and root length and biomass production irrespective of landraces. The principle component analysis (PCA) resulted in the formation of three different clusters as salt sensitive, salt tolerant and moderately tolerant/sensitive suggesting that there was considerable genetic variability for salt tolerance in the local landraces. Among the 26 landraces maximum reduction in germination percentage, seedling growth and biomass production was observed in ST-ST-RO and minimum in ST-WA-VA. The decreased in germination percentage, growth of the seedlings and biomass production showed that ST-WA-VA is salt tolerant and ST-ST-RO is salt sensitive landraces. Key words: NaCl, germination, seedling growth, biomass production

PO – 59 Study of effect of flower’s extract of Calotropis gigantea on angiogenesis by Chorio allontoic membrane assay (CAM) in chick embryo

M. M. Kandar, S. B. More*, R. V. Yadav Department of Zoology and Fisheries, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Calotropis gigantea family Apocynaceae commonly known as madar in hindi is a perennial herb with a long history of use in traditional medicine. C. gigantea is a common wasteland weed and known for various medicinal properties. C. gigantea contain chemical constituents are cardenolids, flanoids, terpenes, pregnanes and non-protein amino acid. In the present investigation effect of flowers extract in ethanol and distilled water C. gigantea on angiogenesis was studied. Fertilised eggs off Gallus gallus were incubator at 38 0C and 72-75 % related humidity. In 48 hrs, 72 hrs and 92 hrs embryos CAM was exposed to 0.25 mg/ml flowers extract in ethanol as well as distilled water and were further incubated up to 144 hrs and CAM was studied. Key words: Calotropis gigantea, angiogenesis, Gallus gallus

PO – 60 Biodegradation of Cellulosic Waste by Aspergillus spp.

S. R. Jaygonawar, T. K. Ulhalkar, V. S. Patil* Department of Microbiology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Cellulose is the major constituent of all plant materials and the most abundant organic molecule on the earth. It is a linear biopolymer of glucose molecules. Degradation of plant cell wall polysaccharide is of major importance in food and feed, beverage, textile and pulp industries, as well as in the several other industrial production processes. Cellulose is not toxic or directly harmful but our major waste disposal facilities landfills are rapidly filling up. So, its ecofriendly degradation is essential. Soil sample is collected from different locations nearby ‘Kaas Plateau’. Cellulose producing fungus was isolated from soil which is identified as Aspergillus spp. Isolated fungus has strong ability to produce cellulase enzyme. This study was confirmed by using Carboxy Methyl Cellulose agar plates. Clear zone was observed around the inoculated fungus. Cellulose degradation ability of fungus was checked by using different cellulosic waste. This study has future prospective for waste management and applications in various industries. Key words: Aspergillus spp., cellulose, cellulase

PO – 61 Bioremediation of oil contaminated soil

Bitale Tushar Krushnat, Deshmukh Anil Ramchandra, Kate Rahul Vitthal, Uttekar G. V.* Department of Microbiology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Petroleum is the major source of energy for various industries and daily life. Releasing petroleum into the environment whether accidentally or due to human activities is a main cause of soil pollution. Soil and surface water contamination is common occurrence in most developing countries. This has been harmful effect on the environment and human beings. Bioremediation can be alternative green technology for remediation of such hydrocarbon contaminated soil. The crude oil sample was collected from motor garage. The sample was analysed for oil contaminated organisms by using minimal salt agar medium. The degradation of oil contaminated soil was observed using minimal salt broth medium by isolated organism and incubation period is 7 days. The procedure of transfer of 1ml from first medium to second fresh medium and these procedures repeatedly by three times. The isolated organisms strain shows activity in oil contaminated degradation within 4-5 days. The analysis of oil contaminated soil degradation by using selected organisms in minimal salt broth medium was observed. In present work oil degradation was studied by using isolated organism. Key words: Degradation, crude oil, environmental effect

PO – 62 Median lethal toxicity of fungicide propiconazole on fish Catla catla

Koli S. S.*, Dagade D. R.*, Bhatia P. R., More R. B. Department of Zoology and Fisheries, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected] [email protected]

ABSTRACT Pesticides have been commonly used in agriculture for gaining more yield from farm. Different types of pesticides are used but commonly used are fungicide, insecticide, herbicide etc. Propiconazole is a fungicide used to control fungus. Propiconazole is continual in environment causing risk to aquatic biota. The present study was aimed to investigate the acute toxicity of Propiconazole fungicide on freshwater fish Catla catla. The toxicity concentration of Propiconazole for 24 to 96 hrs was found to be 12.6 and 8.2 mg L-1, respectively. The present study indicates that Propiconazole is harmful which show lethal effects. It affects non targeted aquatic flora and fauna and also causes water pollution. Key words: Propiconazole, Fungicide, Catla catla, LC50

PO – 63 Antibacterial activity of zinc oxide nanoparticles synthesized using Ocimum sanctum against oral pathogens

Harshada Desai, Dhanraj Nimbalkar* Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Nanotechnology has offered great possibilities in various fields of science and technology. It has been playing crucial role in the development of modern material in recent years. The antifungal activity of zinc oxide occurs by the deformation of the fungal hyphae and preventing the development of conidiophores, leading to the death of fungal hyphae causing cellular destruction. The process includes preparation of plant extract, synthesis of nanoparticles, characteristics of nanoparticles, preparation of nanoparticles powder, and antibacterial activity of nanoparticles against oral pathogens are used. The expected result is zone of inhibition can be increased or decreased with increase in concentration of ZnO nanoparticles. Key words: Zinc oxide nanoparticles, Ocimum sanctum, oral pathogens, Antimicrobial activity

PO – 64 Analysis of Physico-chemical parameters of Kanher Dam, Satara, Maharashtra, India

Uttamrao L. Shevale1,2*, Sandhya M. Pawar2 1Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India 2Department of Zoology, Lalbahadur Shastri College of Art, Science and Commerce, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT The present investigation deals with the physico-chemical parameters of water of Kanher dam on river Venna a tributary of river Krishna in district Satara. The water from the dam is used for drinking, agricultural purposes, electricity generation and aquaculture practices. The present work was carried out from July 2018 to June 2019. The physico-chemical parameters were analyzed such as Temperature, pH, Carbon Dioxide (CO2), Dissolved Oxygen (DO), Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Total Dissolved Solids (TDS), Total Solids (TS), Total Suspended Solids (TSS), Phosphates, Chlorides, Alkalinity, Electrical conductivity, Total hardness etc. These physico-chemical parameters were analyzed by standard methods APHA 1992, Trivedi and Goel 1984. The data was interpreted during study period in relation to pollution status and water quality. The number of parameters was within permissible limit of WHO and the results showed significant seasonal alterations in the physio-chemical parameters of water. Key words: Physico-chemical parameters, Kanher dam water, Electrical conductivity Phosphates, Chlorides, Alkalinity, Chemical Oxygen Demand, Total Solids

PO – 65 Production of bioplastic by microorganisms isolated from soil sample

P. S. Chavan, R. A. Sontakke., P. D. Jagdale., S. S. Kanase*, P. B. Zambare Department of Microbiology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Bioplastic is secondary metabolite produced from microorganism which replaces synthetic plastic in future, it is biodegradable, eco-friendly, biocompatible and microbial thermoplastic. Bioplastic is used in packaging industry, textile industry, manufacturing industry, agriculture, medical industry. In order to isolate and characterize PHB producing organism, soil sample collected from petrol pump, garage, crop field and from curd. Twenty-one organisms were obtaining and screened by using Sudan Black B stain out of which 6 organisms’ potent producers of bioplastic. After incubation of 4 days these organisms were cultured in nutrient broth for 4 days at 370C in rotary shaker incubator, six organisms were further processed for extraction process by hypochlorite-chloroform method. Key words: Bioplstic, Biodegradable, Poly-hydroxybutyrate (PHB)

PO – 66 Present status of fish consumption in Satara City

M. D. Sawant, S. S. Magade, V. Y. Deshpande* Department of Zoology and Fisheries, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT The survey was conducted during the period of 2016-Nov 2018 at various regions of Satara city through personal interviews to access region specific consumption rates by targeting the residence of the respondent. The relevant questions were asked to know knowledge of the respondents of a particular population. The purpose of the survey was to study consumer attitude towards fish consumption that can be currently determined by parameters such as price, taste, quality, availability or hygiene at point of sale. The survey resulted in some interesting observations. In order to satisfy increasing demands many marketing strategies could be evolved and, simultaneously, help could be offered for the development and management of fisheries. Key words: Respondent, consumption, consumers, productivity, key influence

PO – 67 Ethnobotanical study to determine potential medicinal value of Kalanchoe bhidei T. Cooke

R. A. Shinde1,2*, S. V. Thigale1, K. B. Pawar2 1Department of Botany, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India 2Department of Botany, Shivaji University, Kolhapur – 416 004, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Kalanchoe is one of the largest genus of family Crassulaceae with more than 100 species distributed in Asia, Madagascar and Tropical Africa. The genus is represented by about 24 species in India. One of the species, Kalanchoe bhidei T. Cooke is endemic to Western Ghats. It is a succulent, erect, fleshy herb with pale red-colored leaves and white tetramerous flowers appearing in cymose inflorescence. The plant habitat is in the upper reaches of the hills around Satara. The extreme climatic conditions to which the plant is exposed brings out the possibility of accumulation of secondary metabolites which might have therapeutic applications. In order to determine the potential medicinal value of the plant an ethnobotanical survey was undertaken by questionnaire method. The local herbal practitioners and shepherd community people were focussed on to know about the local use of the plant. Many Kalanchoe species have been reported to have toxic effects on animals. Our survey indicates this plant is edible not only by humans but also relished by animals. Topical application of plant as a poultice is used for its wound healing properties. This plant seems to have medicinal properties which needs to be validated scientifically. Qualitative analysis of the plant hint towards presence of large amounts of alkaloids and flavonoids. From this survey we conclude that, this plant is used to cure wounds, urinary disorder and cough also. We can use this plant for animal in case of loss of appetite, to cure wounds and also in urinary infection in cattle. One important information that came up with the survey was the loss of the plant’s population in many of the habitats due to anthropogenic activities. The loss of habitats of this plant poses a threat not only to the plant but also points out to the need of measures to ensure its sustainability. Key words: Kalanchoe, Crassulaceae, ethnobotanical survey

PO – 68 Effects of Natural fungicide neem oil on Catla catla

Uddesh N. Kanase*, Chaitrali K. Gujar, Vishwas. Y. Deshpande Department of Zoology and Fisheries, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Now a day’s pollution is a severe problem in developing countries pesticides are responsible for bioaccumulation in aquatic organisms. Now a day’s farmers are using natural fungicide like neem oil. the present study deals with the effect of neem oil on Catla catla fish were exposed to different concentrations of diclofenac at 96 hrs. And mortality of fishes was observed. The histological changes were observed in selected organ such as gill, liver and kidney as compare to control.

PO – 69 Study of Antimicrobial Activity of Glycyrrihiza glabra and Cymbopogan citrarutis

Rutuja Rajkumar Khurape*, C. C. Shete Department of Biotechnology, Kasturbai walchand college, Sangli, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT The plant species Glycyrrihiza glabra and Cymbopogan citrarutis both has been reported with a microbe resistant quality. Mostly they are used for cough and cold since ancient days. The plant origin herbal medicines are considered as a safe alternative without side effects than a synthetic drug. There are various methods of medicines like Ayurveda, homeopathy and unami, which uses plant extracts to prepare medicines. Dry sticks of liquorice are usually preferred when person is suffering through cough and even it is can be used in powder form by grinding it or by making its extract. It is also used for certain skin problems. In many skin products liquorice is used. Lemongrass is beneficial for sour throat or in cough and cold. We can make tea from it and patient can consume it. We can use it even in powder form. This study is a preliminary evaluation of antimicrobial activity of “Glycyrrihiza glabra and Cymbopogan citrarutis”. It indicates that this plant has potential to kill micro-organisms. The crude extract demonstrated antimicrobial activity against many infectious bacteria. It may help to discover new drugs that could serve as selective agents for maintenance of health.

PO – 70 Isolation and characterization of endophytic bacteria isolated from different medicinal plants

Sonal Suresh Mali*, Jangam S. S. Department of Microbiology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT The role of Neolamarchia cadamba endophytic microorganism in plants can be divided two categories based on types of activity- grow Azadirachta indica the promotion & disease control. True seeds can also be the source of endophtic bacteria. Plant are host to one or more endophytic microorganisms. In the present study, endophytic bacteria were isolated from leaves of Neolamarchia cadamba (kadamba), Azadirachta indica (Neem), Hibiscus rosa-sinensis (Hibiscus), Saraca asoca (Ashoka) four strains were isolated and were studied for their Gram nature and colony characters. These bacterial isolates were tested for their ability to degrade biological, textile dyes and screened for different PGPR traits like phosphate solubilization, production of auxin (IAA), nitrogen fixation and sulphate oxidation. They were also tested for the production of extracellular enzyme like urease. The result of isolates bacteria is positive to phosphate solubilization, the bacterial colony shows zone of tricalcium phosphate solubilization. In production of IAA in broth development of pink colour. The nitrogen fixation on agar plate yellow colour producing colony. In sulphate oxidation test the broth colour changes in yellow, green, light green & decrease the pH range of broth it indicates positive for sulphate oxidation. Urease test in the slant colour develop in reddish pink, orange colour is positive & maximum 59% dye degrade. Keywords: Endophytic bacteria, Dye degradation, PGPR traits

PO – 71 Relative toxicity of extracts of two plant against Corcyra cephalonica (Stenton) under laboratory conditions

S. P. Zanke Department of Zoology and Fisheries, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Plant metabolites are non-nutritive plant chemicals that have protective or disease preventive properties. Plants produce these chemicals to protect them self against the pests. Leaf extracts of two plants Perkinsonia aculata and C. auriculata extracted in acetone were tested against larvae of Corcyra cephalonica (stenton) and were found to be toxic to the larvae. The toxicity effect of these plant extracts resulted in blackening and death of the larvae. The acetone extract of these plants can be used to control the Corcyra cephalonica infestation in stored grain. Keywords: Plant metabolites, infestation, Corcyra cephalonica

PO – 72 Isolation, characterization, screening and antibiotic sensitivity of Actinomycetes from locally collected soil samples

Rutuja Dattatraya Thorat, Dhumal D. D. Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT In the present study, the soil sample was collected from near fields. Actinomycetes strains were isolated in specific medium using Arginine Glycerol Salt (AGS). These Actinomycetes were screened with regard to potential against Gram-positive and Gram-negative bacteria. The purified Actinomycetes strains were performed tests such as, Fermentation of citrate, catalyse test, Vogus-proskauer, Urease test etc. The positive isolates were immobilized using sodium alginate beads and the immobilized cells were cultivated in specific fermentation liquid medium for 120 h. Antibacterial compounds was purified from the filtrate by solvent extraction method. A comparative study on the total antibiotic sensitivity of the free cells and immobilized cells showed that the immobilized strains were be more efficient against the tested microorganisms. The actinomycetes strains were prepared in crude protein and the crude protein was determined by using SDS PAGE method. From the result, we are concluded that the positive immobilized Actinomycetes strain showed high antibacterial activity against tested pathogens.

PO – 73 Screening and isolation of protease producing bacteria from soil

Ghorpade Anuja Vijaysinh*, U. L. Shevale Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT To produce environmental eco-friendly products and product out puts chemical process are being replaced by enzymes like proteases. The production of enzymes is central to the modern biotechnology industry. The objective of present study was to screen and isolate protease producing bacteria from soil. Soil samples were serially diluted and 0.1 ml of sample was spread on skim milk agar, at 37 °C. For 48 hrs. The bacterial colonies showed clear zone around the colony indicating protease activity. The isolate produced highest protease activity and was identified as Bacillus sp. By morphological and biochemical test. Various physiological characters were studied like pH, Temperature, fermentation time. The protease activity at pH and temperature will be examined. The unknown concentration of crude protease will be determined using tyrosine standard curve. The above results indicate that these bacterial isolates can be use as biotechnological tool for industrial purpose. Keywords: Protease, soil, maximum activity, bacteria, fermentation time, tyrosine, standard curve, detergent

PO – 74 Isolation of bacterial isolates from rotten fruits for the Production of bioethanol

Amruta Tarade, Salim Mujawar* Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Bioethanol can be produced from carbohydrate containing substrates by the process of fermentation. Many microorganisms like bacteria, fungi and yeast involved in bioethanol production. Rotten fruits serve as potential substrate for bioethanol production due to the low cost and high sugar contents, vitamins and minerals. At present, bioethanol produced from biomass is alternative fuel for future generation. In present study bioethanol was produced by using rotten fruits as a fermentation medium with inoculation of bacterial isolates isolated from the fruits itself. Ten bacterial strains were isolated from combination of rotten apple and sapota and combination of banana and guava. Among the isolated bacterial strains, five strains named AS1, AS4, BG1, BG4 and BG5 were found to be sfermentative by Jones test. The Gram’s test of these five isolates indicated that AS1 and BG1 were Gram’s positive, while, AS4, BG4 and BG5 were Gram’s negative. Static fermentation was carried out by inoculating five ml of bacterial suspension of all five fermentative bacterial isolates into eight different flasks containing 20 % rotten fruits suspension as a fermentation medium and kept incubated for 6 days at 37 0C in incubator. From 3rd day onward of incubation, two flasks were harvested till 6 days. The amount of ethanol produced was estimated by using potassium dichromate colorimetric method and was found to be 4.1, 5.7, 6.8 and 4.8% on 3rd, 4th, 5th and 6th day, respectively. The highest amount of bioethanol produced was 6.8% on the 5th day of fermentation. Above results indicates that the isolated strains have good potential for production of bioethanol. Keywords: Fermentation, Bacterial isolates, Bioethanol, rotten fruits, Biofuel

PO – 75 Low-cost production of Metamerism anisoliae and Beauveria bassiana, Volvariella volvacea for inoculum development (Bioinsecticide) using solid state fermentation

Yogini Suresh Lawand*, V. M. Nalawade Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara – 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT Fungal fermentation metabolites have an enormous impact on the food, pharmaceuticals and agriculture industry. The basic production of fungal products has largely been in solid state fermentation (SSF). SSF can be defined as the growth of microorganism on moist, water-insoluble soild substrate in the absence or near absence of free liquid. The invention relates to process for producing biopestisides based on Volvariella volvacea, Beauveria bassiana and Metamorisam anispoliya comprising preparing mass or stock culture of biocontrol fungi on sawdust, soil and molasses mixture and immobilizing the bioagent in a flyable based carrier. Beauveria bassiana by solid-state fermentation was studied for the development of a biocontrol agent. Metarhizium anisopliae, formerly known as Entomophthora anisopliae (basionym), is a fungus that grows naturally in soils throughout the world and causes disease in various insects by acting as a parasitoid and Volvariella volvacea (also known as paddy straw mushroom or straw mushroom). FSSF will be used as an abbreviation for fungal solid-state fermentation in this process. An overview of the various factor which affect the development and application of FSSF system with reference to the growing body of literature will be done.

PO – 76 In vivo antiangiogenic potential of curcumin nanoparticles using chick chorio-allantoic membrane assay (CAM)

Sawant M. D.*, Bendre N. N., Deshpande V. Y. Department of Zoology and Fisheries, Yashavantrao Chavan Institute of Science, Satara 415 001, Maharashtra, India *Corresponding Author E-mail: [email protected]

ABSTRACT The aim of the present work is to study the angiogenesis activity of original curcumin compound and curcumin nanoparticles because of its nontoxic, potent and medicinal properties of turmeric. Nanoparticles of bioactive compounds were prepared by controlled re-precipitation technique and were found to have a narrow particle size distribution in the range of 50-100 nm. The synthesized nanoparticles were characterized by UV-Visible spectroscopy, FTIR spectroscopy, NMR spectroscopy XRD, FESEM and TEM techniques. In vivo anti-angiogenic potential of nanoparticles of bioactive compounds was evaluated using Chick Allantonic Membrane (CAM) assay and was compared to that of original compound. It was found that the aqueous dispersion of nanoparticles exhibits much more pronounced anti-angiogenic activity than the original compounds. In fact, anti-angiogenic activity of nanoparticles was found to be markedly improved by particle size reduction up to the nano range. Key words: Curcumin nanoparticle, Anti-angiogenesis, Secondary vitelline veins, Tertiary vitelline veins

PO – 77 Physiological and biochemical analysis of nine Asteraceae weeds from Satara district

Y. R. Chavan* and B. A. Kore Department of Botany, Yashavantrao Chavan Institute of Science, Satara (Autonomous), (MS), India *Correspondence: [email protected]

ABSTRACT Members of family Asteraceae are well known for rich phytochemical content. The study of nine weeds from this family Acanthospermum hispidum DC., Artemisia nilagirica (C. B. Clarke) Pump., Bidens biternata (Lour.) Merr & Sherff., Chromolaena odorata (L.) R. M. King & H. Rob., Cyathocline purpurea (Buch. – Hum. Ex D. Don) Kuntze., Erigeron bonariensis L., Lagascea mollis Cav., Synedrella nodiflora (L.) Gaertn. and Xanthium strumarium L. has been done with respect to their phytoconstituents. These weeds are well-known for their faster growth, biomass production and presence of different types of aromatic compounds. In earlier ethnobotanical study, medicinal values of these weeds were reported. Present attempt reveals its chlorophyll content, carotenoids content, Total Phenolic content (TPC), Total Flavonoids content (TFC), ash, moisture and carbohydrate content. The study also may provide some clues to understand the mechanism of their utilization and control. Key words: Phytochemical analysis, Chlorophyll, Carotenoids, Carbohydrate.

PO – 78 Phytochemical Analysis of Figs and Root exudate of Ficus racemosa.

Monali J.Pawar, Yogesh R.Chavan* Department of Botany, Yashavantrao Chavan Institute of Science, Satara (Autonomous), (MS), India *Correspondence: [email protected]

ABSTRACT The plant Ficus racemosa Linn. belongs to Family Moraceae popularly Known as the cluster fig tree or Gular. It is famous medicinal plant grow along streams and river banks, also cultivated. The plant has been used from long back in Ayurveda, The ancient system of Indian medicine, for various diseases and disorders including diabetes, liver disorders, diarrhea, inflammatory conditions, respiratory and urinary diseases. Root exudate or sap is used in Ayurvedic medicine, while wood used as timber, unripe receptacle are boiled and used for vegetable preparation. Nutritional studies in clued qualitative estimation of protein, free amino acid, Tannin, Alkaloid. The quantitative test of Figs (inflorescence) and root sap shows presence of Phenol, Flavonoids, and carbohydrates. Keywords: Ficus racemosa Linn. Phenol, Flavonoids, Alkaloid, Carbohydrates.

PO – 79 An overview of various aspects for Green Synthesis of copper nanoparticles

Kashid Yaminee. R.1. Supanekar Shirishkumar.V. 2 1Research Scholar Rayat Institute of Research and Development, Satara, Maharashtra, India 2Associate Professor at Willingdon College, Sangli, Maharashtra, India *Correspondence: [email protected]

PO – 80 Antibacterial activity of Eclipta prostrata leaves (L.)

Rutuja Repal*, Riddhi Jog Department of Biotechnology, Smt. Kasturbai Walchand College, Sangli (MS), India *Correspondence: [email protected]

ABSTRACT Eclipta prostrata is commonly known as False Daisy, Bhringraj and Maka. It is a moisture loving herb commonly found on roadside. It is also known as “King of hair’’ used in indigenous system of medicine as a hepatoprotective drug. A wide range of chemical compounds including alkaloids, flavonoids, polyacetylenes and their glycosides have been isolated from this species. Extract and metabolites from this plant have been known to possess pharmacological properties. Eclipta prostrata is an important medicinal plant and its different parts are famous for the treatment of different health problems including digestion, headache, asthma, cough and normalizing skin colour. Keeping in view the important of Eclipta prostrata, it was analyzed quantitatively, qualitatively for its phytochemicals, physiochemical and anti-fungal activity. Keywords: Antibacterial activity, Leaves, Plant extract, Inhibition zone, Eclipta prostrata.

PO – 81 Isolation and Identification of Plastic degrading bacteria from soil.

Maheshwari Shinde, Vishakha Patil Department of Biotechnology, Yashwantrao Chavan College of Science, Karad (MS), India *Correspondence: [email protected]

ABSTRACT Microorganisms play a very important role in the biological decomposition of various materials in the natural environment, this is called biodegradation. Synthetic materials including plastic and polyethylene waste accumulates in the environment and poses an ever increasing ecological threat. Biodegradation of these plastic wastes using potent microbial strains could provide a solution to the problem. In the present study, the in vitro biodegradation of polyethylene and PVC strips were analyzed using the microorganisms isolated from the soil over a period of 1 month of incubation. The microbial species associated with the degrading capibilities were identified as one Gram (+) ve and one Gram (-) ve bacteria. The efficiencies of these two bacteria in the degradation of plastics were compared and one with the higher degrading capacity is identified to be Bacillus cereus strain. Keywords: Biodegradation, Plastics, PVC, Bacillus cereus

PO – 82 Biosynthesis of silver nanoparticles from Agave sisalana, characterization and it's antibacterial activity.

Akshata S. Takade, U. B. Waghmare Department of Biotechnology, Smt. Kasturbai Walchand College, Sangli (MS), India *Correspondence: [email protected]

ABSTRACT In this study rapid, simple approach was applied for synthesis of silver nanoparticles using Agave sisalana root extract.The plant extract acts both as reducing agent as well as capping agent. The silver nanoparticles were synthesized from agave sisalana root by green chemistry and characterized by UV-Vis spectroscopy. From our results it is proved that synthesized nanoparticles solutions show maximum absorption at 300-500 nm in UV-vis spectroscopy. These results showed that silver nanoparticles exhibit remarkable antibacterial activity against tested human pathogens. Nanoparticles showed highest antibacterial activity against salmonella paratyphi and lowest in case of staphylococcus aureus. The antibacterial property against S.aureus is significant observation as equally resistant to many of common medicines used in chemotherapy. Keywords: Biosynthesis, Plant extract, Agave sisalana