Supplementary material, Table A. A complete list of transcripts that significantly changed in TK6 cells upon MNNG treatment

GeneBank Title Function1 Fold2 SCORE3 Access. No change

UP-REGULATED M29877 L-fucosidase α Glycan metabolism 30.82 7 AF001691 periplakin shape control 20.21 6 AL022310 TNF SF 4/OX40L Cell growth control 16.51 6 L24564 RRAD (small GTPase) 14.68 4 U33202 MDM2-D Cell cycle ( nuclear export) 12.71 4 U02388 CYP4F2 Leukotriene metabolism 12.03 6 W47047/AI557295 P8 Candidate for metastasis 11.01 5 AB000584 MIC-1 Growth suppression 10.86 7 AF010309 Pig3 10.48 7 L14813 CEL like Cholesterol metabolism 9.34 4 X16354/S71326 CEACAM1 Growth suppression 9.26 6 HG2724/S62138 Tls/Chop Fusion oncogene 8.52 6 J00219/X13274 INFγ Immune response (growth suppression) 8.01 5 U55054 SLC12A4 Small molecule transport 6.96 4 Y00815 LCA homolog, LAR Signal transduction (growth control) 6.33 5 M54994 CEL Cholesterol metabolism 6.13 4 AF010310 PRODH Proline metabolism 5.97 5 U33203 MDM2-E Cell cycle (P53 nuclear export) 5.58 6 M14083 PAI-1 Extracellular matrix (senescence) 5.57 6 AA635153 ZNF 79 5.44 5 AF022116 AMPK-β1 Kinase 5.31 6 AA586695 PVT1 Oncogene 5.05 6 D12620 CYP4F3 Immune response 4.93 7 AF059611 NRP/B (Pig10) Neurogenesis (growth control) 4.81 7 U78305 WIP-1 Signal transduction 4.8 7 U57317 P/CAF Acetylation (cell cycle control) 4.75 4 AF038844 DUSP14/MPK6 MAPK inactivation 4.66 7 X13839 Vascular smooth α actin Cell shape control 4.65 7 U83461 hCTR2 Ion transporter 4.46 6 X78565 Tenascin C Cell adhesion 4.37 4 D21089 XP-C DNA repair 4.31 7 U18300 XP-E/DDB2 DNA repair 4.27 7 U22963/AF031469 Mhc Rel 1 Immune response 4.08 6 U72649 BTG 2 Growth suppression 4.03 7 M60974 GADD45 Cell cycle arrest 3.91 7 U03106 p21/Waf1 Cell cycle arrest 3.89 7 AF050110 TIEG Growth suppression 3.86 7 U91512 Ninjurin1 Neurogenesis (growth control) 3.77 7 U70426 A28RSG14p Signal transduction 3.73 7 W27419 h ret cDNA EST 3.65 7 X73113 MY BP 2 Cytoskeleton 3.58 6 U59913 SMAD5 Transcription factor (Growth suppression) 3.57 6 U33199 MDM2-A Cell cycle (P53 nuclear export) 3.57 6 U59209 UDP glycosil transferase Metabolism 3.54 4 U24578 C4A Complement component 3.54 6 AF070614 SCHIP1 Structural protein 3.53 6 U62435 Nicotinic rec precursor Colinergic 3.51 6 J03826 FDXR Metabolism 3.51 7 L19871 ATF3 Transcription factor (stress response) 3.46 6 U90913 TIP1 TAX interaction protein 3.46 7 U09825 ZNF 173 Transcription factor 3.38 7 J04076 EGR2 Transcription factor 3.34 5 AI828210 KIAA0284 Unknown 3.24 4 U31382 GNG4 Signal transduction 3.22 5 AF038204/AI830496 KIAA1240 Unknown 3.2 4 J03060 β-glucocerebrosidase Metabolism 3.17 4 AB006782 Galectin iso Immune response 3.14 7 Y10313 IFRD1 Immune response 3.13 6 U76010 SLC30A3 Transporter (zinc) 3.1 7 AB014534 Astrotactin 2 Cell adhesion 3.1 5 AL049538 RIN2/Ras interactor 2 Endocytic pathway 3.1 6 U68494 hbc647 sequence Unknown 3.09 7 M30474 γGT2 Metabolism 3.01 7 U88629 ELL2 DNA replication 3 6 U00672 IL 10R Signal transduction 2.98 6 J04131 γGT1 Metabolism 2.96 6 X61123 BTG1 Growth suppression 2.95 7

1

W27541 CEP4 Cell shape 2.94 7 D87434 KIAA0247 Uknown 2.9 7 U29332 FHL-2 Cytoskeleton 2.88 6 AA663800 LOC51257 (Hypot. Prot) ZN finger domain 2.87 6 X84373 NRIP 1 Transcription factor 2.87 6 AB015718 STK10 Signal transduction 2.87 7 D87002 Igλ chain Immune response 2.87 7 J02969 Tyriod peroxidase Metabolism 2.85 4 U55312 GPR19 Signal transduction 2.84 4 AB011155 DLG 5 Signal transduction 2.84 6 X57348 14-3-3σ Cell cycle arrest 2.81 7 U48705 DDR1 Signal transduction 2.8 7 D87446 KIAA0257 Unknown 2.77 7 U82987 PUMA Apoptosis 2.76 7 X83490 Fas (∆3,4) Apoptosis 2.75 7 AB002313 PlexinB2 Signal transduction 2.75 7 L00635 FNTB Metabolism 2.73 4 W74442 KIAA1794 Unknown 2.72 7 S81439 EGR alpha Cell growth control 2.7 6 AB007455 TP53TG1 Cell growth control 2.7 6 J05428 UGT2B7 Lipid metabolism 2.7 4 X83492 Fas (∆4,7) Apoptosis 2.68 6 AF060219 CHC1L condensation 2.66 6 AF062075 Leupaxin Cytoskeleton 2.66 6 X60201 BDNF Cell growth control 2.63 5 AF091084 phorbolin-like Cytocrome b5 reductase 2.62 4 AA442560 APOBEC3G Immune response 2.6 7 W28743 PP1628 Unknown 2.59 6 AC005192 IFRD1 Development 2.57 6 AL050078 DKFZp566G0746 EST 2.57 5 W20138 STARD5 Lipid metabolism 2.56 5 U48437 APLP1 Neurogenesis 2.55 4 AJ011001 GPR56 Signal transduction 2.53 7 AB018259 KIAA0716 Unknown 2.52 4 AB017915 CHST3 Carbohydrate sulfontransf.erase 2.51 4 L07597 RSK1 Signal transduction 2.5 7 X52560 C/EBPβ Immune response 2.48 7 AB020649 KIAA 0842 Unknown 2.47 6 U10485 LRMP Cytoskeleton 2.46 6 L35545 EPCR Immune response 2.45 5 AF038202 clone23570 Unknown 2.44 6 U68019 SMAD3 Transcription factor (Growth suppression) 2.41 6 X99656 EEN Cell growth control 2.38 6 L22475 Bax−γ Not clear role in apoptosis 2.37 7 D90144 CCL3 Immune response 2.36 7 U33822 MAD1L1 Mitosis checkpoint 2.36 4 M12959 TCRα Immune response 2.35 7 U64820 MJD Cell growth control 2.35 5 D50419 ZNF 175 Transcription factor 2.34 4 M19722 c-fgr Signal transduction 2.33 7 AB002352 KIAA 0354 Unknown 2.33 7 M27533 Ig rearrang B7 Immune response 2.33 6 M60278 EGF-like Cell growth control 2.32 5 AF053356 GNB2 Signal transduction 2.31 6 D87432 SLC7A6 Amino acid transporter 2.31 6 L29277 STAT 3 Signal transduction 2.29 7 X69086 Utrophin Cytoskeleton 2.29 5 M59465 TNFAIP3 Signal transduction 2.28 7 L41913 Rb1 exon26 Cell growth? 2.27 6 U83981 GADD34 Cell cycle arrest 2.26 6 Z74792 NFYC Transcription factor 2.25 7 M87503 ISGFG3 Transcription factor 2.23 5 AI830496 KIAA1240 Unknown 2.23 6 U19599 Bax-δ Apoptosis 2.22 7 D26350 ITPR2 Transporter (Ca) 2.22 5 U35459 Bompain Others 2.21 5 L05568/H94881 FXYD2 Transporter (Na/K) 2.21 4 Z70519 Fas (soluble isoform) Apoptosis 2.21 6 AB014608 p53 associated parkin-like Unknown 2.21 6 M97935 STAT1 Signal transduction 2.21 6 AL022238 MKL1 Megakaryoblastic leukemia 2.2 6 U50527 BRCA2 region DNA repair 2.19 6 U11732 TEL Transcription factor 2.19 5 AL022318 APOBEC3 Immune response 2.18 7 X63717/X89101 FAS/APO1 Apoptosis 2.17 7

2

S66427 RBBP1 Cell growth 2.15 5 D14838 FGF9 Growth factor 2.14 5 L38933 GT 198 Unknown 2.14 6 AL022318 phorbolin3 Unknown 2.14 7 AI739308 SRP46 Splicing factor 2.12 6 AF015451 CASP8 prot Apoptosis 2.12 4 L20817 DDR1 Cell adhesion 2.12 7 M36653 Oct2 TF Transcription factor 2.12 5 AI888563 Smoothelin Cytoskeleton 2.11 7 X62078/X61094 GM2 act prot Unknown 2.11 6 U60519 CASP 10 B Apoptosis 2.11 5 U66469 CGR19 (p53dep) Growth suppression 2.11 6 L05072 IRF1 Apoptosis (INFγ pathway) 2.11 7 L22473 Bax-α Apoptosis induction 2.1 7 AJ223948 Rnase H RNA metabolism 2.1 6 AF034956 RAD 51 L3 DNA repair 2.1 5 AF003540 ZNF 195 Transcription factor 2.1 6 U77949 CDC6 Cell cycle regulation 2.09 7 L07594 TGFβ R3 Signal transduction 2.09 6 AI138834 KIAA1528 Unknown 2.08 7 X02883 TCRα/chain C Immune response 2.08 6 L13435 AD017 Glycosyltransferase 2.07 6 AB002304 capicua homolog Cell mobility 2.07 5 L49229 Rb1 exon22 Cell cycle regulation 2.06 7 AI038821 HRAS 1 Signal transduction 2.06 6 U03057 SNL Cytoskeleton 2.06 7 AB002382 Catenin delta 1 cell adhesion 2.06 6 U07358 MAP 3K 12 Signal transduction 2.06 6 U69198 RFNG Extracellular matrix 2.05 4 U89896 Caseine kinase 1 Signal transduction 2.05 6 U64871 GPR 19 Signal transduction 2.04 5 U37518 TNFSF10 Apoptosis 2.04 6 M16967 F5 Coagulation 2.04 5 L16896 HKR3 Transcription factor 2.04 4 AB006624 KIAA0286 Unknown 2.03 6 AB022718 DEPP Unknown 2.03 5 AB011104 KIAA 0532 Unknown 2.02 4 AL050371 PISD Decarboxilation 2.02 7 U90916 clone23815 Unknown 2.02 6 AB000461 4orf8 Unknown 2.01 7 U51240 LAPTM5 Metabolism 2.01 7 U77845 hTRIP Apoptosis 2.01 6 X99209 HMRT1L1 Signal transduction 2.01 5 S52028 CTH Metabolism 2 6 D28588 2 5 AF053004 TCCR Immune response 2 6 X92841 MICA Immune response 2 6 M55531 GLUT5 Glucose transporter 1.99 5 AF001846 PT 22 Signal transduction 1.98 7 M36067 Ligase I DNA repair 1.97 7 AB004922 SMAD3 exon1 Cell cycle regulation 1.97 6 AI458463 PACE-1 Unknown 1.97 6 U56998 Cytokine-induced kinase Cell cycle regulation 1.97 7 J00277 H RAS Cell cycle regulation 1.96 7 AF055479 FUS 1 Cell cycle regulation 1.96 7 HG2441-HT2537 RB mutated Cell cycle regulation 1.95 5 W22541 PCBP4 Cell cycle and apoptosis (P53-ind) 1.94 6 U26648 Syntaxin 5 Vesicle transport 1.94 6 S73591 TXNIP Metabolism (growth arrest-associated) 1.93 7 U66561 ZNF 184 Transcription factor 1.93 5 U34804 STP 2 Metabolism 1.93 6 X78925 ZNF 267 Transcription factor 1.93 6 AB028948 KIAA1025 Unknown 1.93 5 AB020700 KIIA 0893 Unknown 1.93 6 AB023209 palladin Unknown 1.93 6 U90551 H2A Histone family 1.92 5 U51127 IRF5 INFγ pathway (cell growth control) 1.92 7 AL096739 GALNT10 Metabolism 1.92 5 AF031815 KCNN3 Transporter (K) 1.92 7 AB014543 KIAA0643 Unknown 1.92 5 AB014530 NBAK2 Nuclear kinase 1.92 5 D86963 DVL3 Signal transduction 1.92 7 U16811 Bak Apoptosis 1.92 6 AA133246/M81830 LAIR2 Unknown 1.91 6 AJ001612 PSPHL Phosphatase (cell growth) 1.91 6

3

D49958 GPM6A Plasmamembrane protein 1.9 5 X95152 BRCA2 exon2 DNA repair 1.89 6 AW051579 FLJ10512 Unknown 1.89 6 Z49878 GAMT Metabolism 1.89 7 AF007130 MDM1 Nuclear protein 1.89 6 Y08200 RABGGTA Protein modification 1.88 7 U00672 IL10Ralpha Signal transduction 1.87 6 M83221 RELB Transcription factor 1.87 7 U81787 WNT10B Signal transduction 1.86 6 AB002303 ENDOFIN Unknown 1.86 5 M85169 PSCD1 Vesicle transport 1.86 7 X61094 GM2 act prot lysosome 1.84 4 M84443 GALK2 Metabolism 1.84 4 AF068180 B-cell killer Immune response 1.84 7 AJ001902 MGC10558 Unknown 1.83 7 U78190 GCHFR NO biosynthesis 1.83 4 Z50194 PHLDA1 Apoptosis 1.82 5 AB018276 MAP3K7IP2 Signal transduction 1.82 6 X85116 EPB72 Cytoskeleton 1.82 6 M92287 D3 Cell cycle (G1/S specific) 1.81 7 S54641 ZNF 124 Transcription factor 1.81 4 M63978 VEGF Cell growth 1.81 6 AB020648 KIAA 0841 Unknown 1.81 7 AF031166 SRP46 Splicing factor 1.81 5 U43374 MGC21654 Unknown 1.81 6 AF032885 FOXO1A Transcription factor 1.8 6

DOWN-REGULATED V00568 c- Cell growth -3.09 7 GENES AI553745 HSPC111 Unknown -3.06 7 U52682 IRF 4 Cell growth -2.67 7 AF026488 Spectrin beta Cytoskeleton -2.64 4 L08177 EBI 2 Immune response -2.51 6 AF070648 Caveolin 1 Plasmamembrane protein -2.49 5 AI799757 Ig heavy chain variable region Unknown -2.48 4 AA224832 Metallothionein1L Heavy metal metabolism -2.39 4 AF023612 DIM1 Cell cycle regulation -2.37 4 D14659 KIAA0103 Unknown -2.36 6 S68805 AGAT Energetic metabolism -2.36 6 AI912041 HSP E1 Protein folding -2.32 7 M59830 HSP70-2 Protein folding -2.28 6 X78669 RCN2 Ca-binding protein -2.21 5 H10776 CBX6 DNA binding protein -2.2 6 X68836 MAT2A Metabolism -2.18 6 X66435 HMGCS1 Lipid metabolism -2.18 6 D78130 Squalene Epoxidase Lipid metabolism -2.15 7 Y00371 HSC 70 Protein folding -2.12 7 M86917 OSBP Lipid metabolism -2.12 4 M15353 EIF 4E Translation -2.1 6 W27466 HNRPDL DNA-RNA binding protein -2.07 6 U28386 KPNA2/SRP 1 Protein transport -2.06 7 U79274 Clone 23733 EST -2.04 6 U15552 HSU15552 Acidic 82KDa protein -2.03 5 Y12478 WRB Nuclear protein -2.02 6 AF084523 CREG Transcription factor -2.02 4 AF055993 SAP30 Transcription factor -2.02 6 X62822 SIAT 1 Protein modification -2.02 7 X60221 ATP5F1 Energetic metabolism -2.01 5 U63825 DIPA Hortolog of hepatitis δ antigen -2.01 7 AB007893 KIAA0433 Unknown -2 5 AF054186 EEF1E1 Metabolism -1.99 5 L31801 SLC16A1 Small molecule transporter -1.97 5 M11717 HSP 70 Protein folding -1.96 5 U23942 CYP51 Lipid metabolism -1.95 6 AI190165 MPP6 Membrane protein -1.95 5 M37197 CBF2 Transcription factor -1.94 6 X81788 ICT 1 Differentiation -1.93 6 M11717 HSP 70 Heat shock protein -1.92 7 U96876 INSIG1 (insulin induced ) Cell growth -1.92 7 M37197 CBF2 Transcription factor -1.91 6 U72514 C2f/yeast mra1 homolog Cell growth -1.91 7 D43950 KIAA0098 Unknown -1.89 7 M25753 Cyclin B1 Cell cycle -1.88 7 X12654 CHC1 Chromosome condensation -1.88 7 X02160 Cell growth -1.87 5 AL049996 HDCMA18P Unknown -1.87 4

4

U23803 HNRPA0 RNA binding protein -1.87 6 AB028974 PEG10 Others -1.87 7 X63679 TRAM Endoplasmic reticulum -1.86 4 X95263 PWP2H Signal transduction -1.86 4 U05259 CD79A Signal transduction -1.86 7 AL080150 GEMIN4 RNA binding protein -1.85 6 D25218 RRS1 Ribosome biogenesis -1.85 6 D14660 MRPL19 Ribosomal protein -1.85 6 AA768912 SSBP1 DNA binding protein -1.85 7 U34044 SPS Protein modification -1.85 5 AL050035 cDNA DKFZp566H0124 EST -1.85 5 D21260 clathrin heavy chain Vesicle traffic -1.85 4 U11791 cyclin H Cell cycle -1.84 7 M90356 BTF3 Transcription factor -1.84 6 J03071 GH1 and 2, and somatotropin Cell growth -1.84 5 AF011333 LY75 Immune response -1.84 5 Z69030 PPP2R5C Signal transduction -1.82 6 D78275 PSMC6 Proteasome -1.82 6 Y12065 NOL5A RNA binding protein -1.8 7 X16983 Cell adhesion -1.8 4 U79526 Chemokine-like receptor 1 Immune response -1.8 4 Y13647 SCD Lipid metabolism -1.8 5 X13482 SNRPA1 RNA binding protein -1.8 7 D78261 IRF 4 INFγ pathway (cell growth) -1.8 6

1 Derived from LocusLink and SwissProt databases or recent publications in case of incomplete annotations 2 Average signal of MNNG-treated TK6 cells vs average signal of untreated TK6 cells 3 See Methods

5

Supplementary material, Table B. Genes differentially expressed in MT1 compared to TK6 cells

GeneBank Title Function/Cetegory1 Fold2 SCORE3 Access. No change UP-REGULATED M59040 CD44 Adhesion (Growth stimulation) 38.01 6 GENES AF030107 RGS13 G-protein coupled signaling modulator 31.29 6 AA919102 CD3D T-cell receptor complex 31.07 6 AA447559 GAGE 7B Tumor antigen (Unknown function) 28.85 6 U19143 GAGE 2 Tumor antigen (Unknown function) 24.16 6 AF058988 GAGE 7 Tumor antigen (Unknown function) 21.96 6 AF070614 SCHIP1 (neurofibromatosis) Structural protein 21.46 6 N55205 EST Hs20205 Similar to hemoglobin ε 19.27 7 M34181 PRKACB (PKA cat sub β) Cell transformation 18.59 6 M25079 β-globin Hemoglobin subunit 15.13 7 M16541 Role in tumorigenesis 14.53 6 U19146 GAGE5 Tumor antigen (Unknown function) 14.34 6 U19145 GAGE 4 Tumor antigen (Unknown function) 12.34 6 AF098641 CD44 (isoform RC) Adhesion (Growth stimulation) 11.38 6 U19180 BAGE B melanoma antigen 11.18 6 X55740 5' DNA metabolism 9.31 5 U84573 Lysine idroxilase Protein modification 8.85 7 X63741 EGR3 Transcription factor 8.63 6 U19147 GAGE6 Tumor antigen (Unknown function) 8.43 6 AF007153 Clone 23736 Unknown 8.41 6 M64174/AL039831 JAK1 Signaling (role in transformation) 8.4 6 U20938 DPYD Pyrimidin metabolism 7.67 5 M64110 CALD 1 Cytoskeleton 6.56 5 X05908 Annexin 1 Anti-inflammatory response 6.41 7 D90144 SCYA3 Inflammatory response 6.07 7 U16799 Na/K ATPase β1 subunit Transporter (small molecule) 5.62 7 X04470 SLPI Serine protease inhibitor 5.46 5 AF062075 Leupaxin Cytoskeleton 4.54 6 X84373 NRIP1 Transcription factor 4.49 6 D10202 PTAFR Signaling (cell growth) 4.47 7 AL031186 Putative emu1 Unknown 4.34 6 D49958 GPM6A Plasmamembrane 4.16 6 Z50194 PHLDA1 Tumorigenesis 3.79 6 AL050159 DKFZP586A0522 Unknown 3.79 6 K03000 ALDH1A1 Metabolism 3.76 6 X86098/AA127624 BS69 E1A binding protein (Transcription) 3.74 4 AF052145 Clone 24400 Unknown 3.67 6 AB002311 PDZ-GEF1 Role in focal adhesion kinase 3.58 6 M55531 SLC2A5 Glucose/fructose transporter 3.55 6 AF038182 clone 23860 Unknown 3.4 7 X13839 Vasc. smooth α actin Cell shape control 3.28 7 M97252 KAL1 Cell adhesion and motility 3.27 6 U15085 HLA-DMB Immune response 3.22 7 X52022 Collagen6A3 Structural protein 3.1 4 W28612 ESTs 3.06 6 AA013087 clone MGC:17296 Unknown 3.05 6 S57212 MEF2C Transcription factor 3 6 AF093239 Pannexin1 Unknown 2.99 6 X02910 TNF Signaling 2.93 7 J04130 SCYA4 Cell adhesion/immune response 2.92 6 AF054589 HIC protein ESTs 2.91 6 U83661 ABCC5 Transporter (organic anion) 2.81 6 X79888 AUH mRNA catabolism 2.81 6 AB016247 SC5DL Lipid metabolism 2.8 6 AB023967 ROD1 Differentiation 2.77 5 U07620 MAPK10 Signaling 2.75 5 D26350 ITPR2 Signaling 2.74 4

6

M87507 Caspase 1 Apoptosis 2.66 6 AL080091 DKFZP564L0862 protein Unknown 2.65 5 D43945 TFEC Transcription factor 2.64 6 Y13493 DYRK2 Kinase 2.64 5 D55649/L28821 MAN2A2 Metabolism 2.59 5 W72186 S100A4 Motility and invasive growth 2.56 6 M33308 Vinculin Cytoskeleton 2.51 7 AL096739 Putative glycosil transferase Metabolism 2.47 4 M55536 Glc transporter pseudogene Unknown 2.46 6 AF070570 clone 24473 Unknown 2.45 7 M14745 BCL2 Apoptosis inhibitor 2.43 6 U52521 Arfaptin1 Structural 2.4 4 AB007925 SRGAP2 Signaling (G-protein) 2.39 4 U03985 NSF Neuroreceptor modulation 2.37 7 D26070 ITPR1 Signaling 2.31 6 AL050006 DKFZP564A033 Unknown 2.31 5 AA010078 H4FD Histone 2.3 6 D87119 GS3955 Unknown 2.29 5 AL021396 DJ971N18.2 Hypothetical protein 2.26 7 AB018356 Sialyltransferase 9 Carbohydrate metabolism 2.25 5 AL050144 ARNIP AR interacting protein 2.25 6 M26683/M28225 CCL2 Signaling/anti-patogen/tumorigenesis 2.25 7 AL050107 TAZ Unknown 2.24 5 Z48579 ADAM10 Metallopeptidase/Signaling 2.23 4 AL049265 DKFZp564F053 Unknown 2.22 6 Z85986 DKFZp451N147 Unknown 2.21 6 U92436 PTEN Putative tumor suppressor 2.18 5 S67334 PIK3CB Signaling 2.17 4 X65873 KIF5B Microtubule function 2.16 6 D49354 PPFIA1 Cell adhesion/signaling 2.14 6 M97935 Iron metabolism 2.14 7 S72869 D10S170 Cytoskeleton 2.13 6 AF070617 DKFZp564D172 Hypothetical protein 2.12 4 U49436 FABP4 (fatty acid BP) Translation 2.11 7 AI760932 CGI-60 Unknown 2.11 6 AJ223352 H2B Histone 2.1 5 L16499 HHEX Transcription factor/development 2.05 6 D13540 PTPN11 Signaling 2.04 6 AL049969 DKFZp564A072 Unknown 2.02 5 U68019 SMAD3 Transcription factor (Growth suppressor) 2.01 6 AJ007014 AMMECR1 Alport syndrome 2 4 AF064093 KEO4 Plasmamembrane 2 5 U17032 ARHGAP5 Cell adhesion/cytoskeleton 2 5

DOWN-REGULATED D87459 WASF1 Cytoskeleton -2 6 GENES U96721 HPS Metabolism -2.01 4 AA461365 RAB4B RAS oncogene family -2.01 5 Y00796 Integrin, alpha L Cell adhesion -2.02 7 M14630 PTMA Transcription (cell cycle reg) -2.02 7 AI148772 Kynureninase Tryptophan catabolism -2.02 6 U79526 CMKLR1 Signaling -2.04 5 N58115 AD024 Unknown -2.04 5 J05032 Aspartyl-tRNA synthetase Protein biosynthesis -2.05 6 AB023227 KIAA1010 Unknown -2.05 6 X55079 Acid alpha-glucosidase Energetic metabolism -2.06 6 AF070552 CDT1 DNA replication factor -2.07 6 S69115 NKG7 Cell surface -2.07 7 J04102 ETS2 Transcription factor -2.08 4 L41668 GALE Galactose metabolism -2.08 6 W25951 Septin 6 Cell division -2.08 6 AB014536 Copine III Lipid metabolism -2.08 6 D16294 ACAA2 Energetic metabolism -2.09 7

7

AJ223321 ZNF238 Transcription repressor -2.1 6 AF054987 Aldolase C Fructose metabolism -2.1 7 M62324 MRF-1 Transcription factor -2.11 6 M60974 GADD45A Cell cycle arrest -2.12 6 M91196 ICSBP1 Transcription (IFNγ pathway) -2.12 7 M94856 FABP5 Lipid metabolism -2.13 6 AB018289 KIAA0746 Unknown -2.13 5 U58516 MFGE8 Cell adhesion -2.14 7 AI039880 UBE2E1 Ubiquitination -2.15 6 M55543 GBP2 Immune response -2.16 5 X56832 Enolase 3 Metabolism -2.16 5 AF004709 MAPK13 Signaling -2.17 5 AF019225 APOL1 Lipid metabolism -2.18 4 U89606 pyridoxal kinase Metabolism -2.2 7 M58285 hematopoietic protein 1 Unknown -2.23 6 M98833 FLI1 Histo-organogenesis -2.23 6 W72424 Calgranulin B Signaling -2.24 4 L41147 HTR6 (serotonin receptor 6) Signaling -2.26 6 M27396 Asparagine synthetase Metabolism -2.26 7 U40705 TERF1 Mitotic control -2.27 6 X51435 HIVEP1 Transcription factor -2.28 5 D32143 Biliverdin reductase B Biliverdin metabolism -2.28 6 AJ006973 TOM1 Lysosomal -2.28 4 U75744 DNase like3 DNA fragmentation -2.29 6 AF023462 PHYH Lipid metabolism -2.31 6 U20158 LCP2 Signaling (kinase) -2.31 5 AB018310 KIAA0767 Unknown -2.34 6 X62078 GM2 act prot Unknown -2.36 5 U29185 Prion protein Pathogenesis -2.37 6 AF054184 Sec61gamma Protein translocation -2.37 7 AB018285 TSGA Transcription factor -2.38 6 AF039945 2 Signaling -2.39 4 U57877 SDHC Krebb's cycle -2.4 7 D87457 ELMO1 Cell motility -2.41 6 AB023147 KIAA0930 Unknown -2.41 6 D28124 DAN Differentiation and apoptosis -2.42 7 AF020314 IRC1 Leukocyte membrane antigen -2.42 6 AJ000342 DMBT1 Potential tumor suppressor -2.42 7 M25629 Kallikrein 1 Peptidase -2.43 6 AI126171 OSR2 DNA binding protein -2.43 4 AB012668 FUT7 Fucosyltransferase 7 -2.43 5 D26155 SMARCA2 Transcription -2.43 6 J03040 SPARC Extracellular matrix -2.47 6 AJ238246 Keratin 7 Cell structure -2.48 5 AI808958 KIAA0870 Unknown -2.48 6 Y12781 TBL1X Signaling -2.49 6 Z84718 Clone CTA-322B1 Unknown -2.49 7 X02994/M13792 Adenosine deaminase Immune response -2.5 7 AF059611 NRP/B (Pig10) Nuclear matrix (neurogenesis) -2.54 7 X70683 SOX4 Transcription factor (apoptosis) -2.56 7 D38128 Prostacyclin receptor Signal transduction -2.56 4 U84371 Adenylate kinase2 Apoptosis -2.58 6 AF038174 FLJ38045 Hypothetical protein -2.6 4 AF055001 HERPUD1 Stress inducible -2.61 6 M34677 F8A (Factor VIII associated) Unknown -2.62 7 AF068180 BLNK Signaling (Growth control) -2.64 7 HG4036-HT4306 Retinoblastoma 1 Tumor suppressor -2.67 6 AF036956 CUGBP2 RNA processing -2.7 5 AF063020 PSIP2 Unknown -2.72 6 AB020689 KIAA0882 Unknown -2.74 7 AL080184 INSIG2 Insulin induced gene -2.75 6 AB028992 KIAA1069 Unknown -2.75 6

8

U03397 TNFRSF9 Apoptosis/growth suppressor -2.79 6 AF026488 Spectrin β Cell shape and size -2.79 4 AF044896 IBC-1 Cell adhesion -2.8 5 U51903 IQGAP2 Signaling and cell shape -2.9 6 L13210 Mac-2 BP Extracellular matrix -2.9 7 X98834 SALL2 Transcription factor (embryogenesis) -3 6 AB014589 CSTF2T Unknown -3.03 6 AA083129 SEC61B Protein translocation -3.04 7 S81914 IER3 Anti-apoptosis -3.09 7 AI768188 cDNA FLJ21243 Unknown -3.1 6 M16505 Steroid Steroid catabolism -3.15 4 AC005954 TJP3 Tight junction -3.16 5 X70297 CHRNA7 Nicotinic receptor subunit -3.16 6 U30894 Sulfamidase Proteoglycan metabolism -3.17 4 AF027826 TM7SF1 Plasmamembrane -3.2 5 L42450 PDK1 Glucose metabolism -3.2 6 AL049417 DUSP3 Signaling -3.21 6 M18533 Dystrophin Structural protein -3.26 6 V01510 POMC ACTH precursor -3.3 4 J02966 SLC25A4 (solute carrier family 25) Energy pathways -3.38 7 AF002697 BNIP3 Pro-apoptotic -3.39 7 M94345 CAPG Cytoskeleton -3.39 6 S68805 AGAT Energetic metabolism -3.4 6 U20536 Caspase 6 Apoptosis -3.47 5 AL008637 NCF4 Energetic metabolism -3.51 4 AB021663 ATF5 Transcription factor (cell cycle control) -3.54 7 S77812 VEGFR1 Signaling -3.57 4 AF078077 GADD45B Cell growth arrest -3.58 6 L37882 FZD2 Signaling -3.63 5 M80899 AHNAK Nucleoprotein -3.66 6 M33552 LSP1 Cell motility and shape -3.67 7 AL041124 PP1665 Hypothetical protein -3.68 4 AB018281 KIAA0738 Unknown -3.68 4 AA133246 LAIR2 Unknown -3.72 6 U71364 SERPINB9 Proteinase inhibitor -3.74 5 X62822 SIAT1 Protein modification -3.79 7 U01134 FLT1 Signaling -3.8 5 U51240 LAPTM5 B cell differentiation -3.86 7 M55150 FAH Tyrosine catabolism -4.05 5 U14394 TIMP3 Extracell matrix maintenance -4.07 6 M64572 PTPN3 Signaling -4.07 5 AL031846 clone RP4-742C19 Unknown -4.14 6 AF052142 Neurocalcin delta Calcium binding protein -4.14 6 AB019517 PKIG Kinase inhibitor -4.16 6 AW052084 KIAA1001 Unknown -4.21 5 M97935 28S ribosomal RNA -4.22 5 X69089 Myomesin (M-protein) 2 Structural -4.23 5 AF002246 CHL1 Cell adhesion -4.26 6 U22431 HIF1A Transcription factor (stress response) -4.27 6 L06797 CXCR4 Chemokine receptor (Growth control) -4.36 7 AF043897 C9orf3 Unknown -4.38 5 U25138 KCNMB1 Transporter (potassium) -4.44 6 AI142565 RNase6 RNA metabolism -4.5 6 AF063002 FHL1 Transcription factor -4.57 6 W07033 GMFG Differentiation (kinase inhibitor) -4.59 6 L20971 PDE4B Apoptosis -4.6 6 AB006780 Galectin 3 Growth control -4.79 7 D31762 TRAM-like protein Unknown -4.79 7 M60278 DTR Signaling (Growth control) -5 5 AB022718 DEPP Unknown -5.07 5 AB011159 NCKAP1 Apoptosis -5.22 4 L05515 CRE-BPa Transcription factor (ATF/CREB family ) -5.24 6

9

AL049801 STARD13 Similar to RhoGAP -5.54 4 AF073308 DFNA5 Unknown -5.54 6 U83171 CCL22 Immune response/Signaling -5.57 7 X07203/M27394 CD20 Pro-apoptotic -5.96 7 U10550 GEM Signaling (Ras-family) -6 6 M28696/X62573 FCGR2B Immune response -6.28 6 X76104 DAPK1 Pro-apoptotic (IFNγ dependent) -7.03 6 X59770 IL1R2 Immune response -7.14 6 AB007854 GAS7 Growth arrest specific -7.23 4 D89974 Vanin2 Cell motility -7.41 4 AF070648 Caveolin 1 Plasmamembrane -7.53 6 S60415/U95019 CACNB2 Calcium channel -7.59 6 L00972 CBS Cysteine metabolism -8.14 6 X69819 ICAM3 Cell adhesion -8.41 6 M36035 BZRP benzodiazapine rec. (Mitochondria) -8.52 7 AA669799/Y15521 ASMTL Acetylserotonin O-methyltransferase-like -9.36 7 U00672 IL10RA Signaling -9.39 6 AA570193 cystatin A Protease inhibitor -10.48 6 M54994 CEL Lipid metabolism -11.12 4 AF079221 BNIP3L Pro-apoptotic (BCL2 inter. Protein) -13.43 7 J02611 Apo D Lipid metabolism -13.9 5 AF072099 LILRB4 Immune response -15.71 6 AJ130718 SLC7A7 Transporter (amino acids) -16.23 6 M25280 L Cell adhesion -17.22 6 AF035819 MARCO Immune response -17.98 6 D64142 H1FX Histone -19.59 5 U65011 PRAME Tumor antigen (Good prognostic marker) -21.27 6 D38549 CYFIP1 Unknown -38.29 6 U03057 SNL/fascin1 Cell shape and size/cell proliferation -41.9 6

1 Derived from LocusLink and SwissProt databases or recent publications in case of incomplete annotations 2 Average signal of untreated MT1 cells vs average signal of untreated TK6 cells 3 See Methods

10

Supplementary material, Table C. Transcripts differentially expressed in WTK1 cells upon 0.4 µM MNNG treatment

GeneBank Title Function1 Fold2 Access. No change

UP-REGULATED D78333 CCT6B Protein folding 9.39 GENES L19314 HES1 Transcription factor (differentiation) 7.77 AF038204 KIAA1240 Unknown 7.46 AA213789 RRN3 Transcription factor (Pol I) 6.93 M94151 Catenin α 2 Cell adhesion 6.83 AF050110/S81439 TIEG Growth suppression (TGFβ pathway) 6.59 AL046961 DKFZp586J0417 EST 6.18 AA133246 LAIR2 Unknown 5.87 HG2724-HT2820 Tls/Chop Fusion oncogene 5.14 AF070621 KIAA1735 Unknown 5 M30257 VCAM 1 Cell adhesion 4.56 S73591 TXNIP Metabolism 4.37 U55312/U64871 GPR19 Signal transduction 4.22 AB007883 KIAA0423 Unknown 4.09 S77812 FLT1 Signaling (growth control) 4.02 AF000994 UTY Anti-pathogen response 3.98 W28567 MEF 2 Transcription activator 3.94 U78722 ZNF165 Transcription factor 3.75 AB011104 KIAA0532 Unknown 3.7 M10905 Fibronectin1 (Alt. Spl) Cell adhesion 3.69 AL080123 ZNF23 Transcription factor 3.69 M25756 SCG2 Protein secretion 3.68 X05323 Mox2 Development 3.68 U64315 ERCC4 DNA repair (NER) )3.67 X16354 CEACAM1 Growth suppression 3.64 M57729 C5 Immune response 3.55 U12535 EPS8 Signaling (EGF pathway) 3.43 U03106 p21/WAF1 Cell cycle arrest 3.41 X92110 HCGVIII Unknown 3.36 X78925 ZNF267 Transcription factor 3.35 AB020717 SYNJ1 Endocytosis 3.35 Y10313 IFRD1 Differentiation (IFNγ pathway) 3.34 J03060 GCB Metabolism 3.32 AB028948 KIAA1025 Unknown 3.31 M73255 VCAM1 Cell adhesion 3.29 U90841 SSX4 Tumor antigen 3.28 M74089 BC017169 Hypothetical protein 3.27 AF001549 Similar to RRN3 ? 3.18 AL022393 P1 p373c6 Hypothetical protein 3.14 S58544 SPAG1 Signaling (spermatogenesis) 3.13 AL049265 DKFZp564F053 cDNA sequence 3.11 U23070 NMA Oncogenesis 3.11 U66561 ZNF184 Transcription factor 3.04 AA151716 APG12L Macroautophagy 3.04 X83492/X63717 Fas/APO-1 Apoptosis 3 U11732 ETV6 Transcription factor (oncogenesis) 2.99 AI635895 DSIPI Transcriptional regulator 2.98 X63741 EGR3 Transcription factor 2.98 U09367 ZNF136 Transcriptional repressor 2.97 M16750 PIM-1 Signaling (growth control) 2.96 U66469 CGR19 Transcription factor (growth suppression) 2.96 AF057164 SLC22A5 Carnitine transporter 2.95 X58072 GATA3 Transcription factor (T cells development) 2.95 X75042 REL Transcription factor (oncogenesis) 2.94 AF078077 GADD45B Growth suppression and apoptosis 2.94 U68494 hbc647 Unknown 2.86 M29877 Fucosidase α Glycan metabolism 2.85 AB018287 HDAC9 Transcriptional regulator 2.84 Y12490 TRIP11 Transcription activator 2.83 U90551 H2AFL Histone 2.8 Y10256 MAP3K14 Signal transduction 2.79 AB023137 AKAP2 Signaling 2.78 AI830496 KIAA1240 Unknown 2.78 HG3044-HT3742 Fibronectin1 Structural 2.77 AF070614 SHIP1 Structural 2.77 Z50194 PHLDA1 Structural (apoptosis) 2.76 U03397 TNFRSF9 Apoptosis 2.75 AI688640 NK-TR Immune response 2.75

11

U33052 PRKCL2 Signal transduction 2.75 D10925 CCR1 Immune response (signaling) 2.75 S66427 RBBP1 RB regulator 2.74 AF070578 SLC38A6 Transporter (amino acids) 2.73 AB026436 DUSP10 Stress response 2.72 AB022918 ST3GALVI Glycolipid metabolism 2.7 S81914 IER3 Anti-apoptosis 2.69 Z29574 TNFRSF17 Proliferation control 2.69 U45878 BIRC3 Anti-apoptosis 2.68 AB007940 KIAA0741 Unknown 2.65 U13616 ankyrin 3 Cytoskeleton 2.65 AL079697 P38IP Transcription factor 2.64 L04510 ARFD1 Vesicles transport 2.63 AB007886 ZNF305 Transcription factor 2.62 U59913 SMAD5 Cell cycle 2.61 D25216 KIAA0014 Unknown 2.6 AF023462 PHYH Lipid metabolism 2.59 U47741 CBP Transduction (growth control) 2.59 X51435 HIVEP1 Transcription factor (Pro-apoptotic) 2.59 AF052099 MTMR9 Unknown 2.59 AF052102 DMP1 Cell cycle arrest 2.59 M60974 GADD45 Cell cycle control 2.58 AC005192 IFRD1 UP in differentiation (IFNγ pathways) 2.58 U37139 ITGB3BP Cell adhesion 2.58 U26710 CBLB Signaling (oncogenesis) 2.55 D89928 ZNF354A Transcription factor 2.53 AJ238246 Keratin7 Cytoskeleton 2.53 AF068227 CLN5 Plasmamembrane 2.52 X78926 ZNF268 Transcription factor 2.51 AF059611 NRPB/pig10 Neurogenesis 2.51 HG2152-HT2222 ZNF92 Transcription factor 2.5 X84373 NRIP1 Transcription factor 2.49 AF041853 KIF3A Motor activity (kinesin complex) 2.48 AF032457 BIM (Bcl2-like) Pro-apoptotic 2.47 D50419 ZNF175 Transcription factor 2.47 AL096713 FER1L3 Membrane regeneration 2.46 U07802 ERF2 Transcription factor (growth control) 2.45 U85658 TFAP2C Transcription factor (cell-cell signaling) 2.44 S52028 CTH Metabolism 2.43 AI184710 1948459 EST 2.43 X78932 ZNF273 Transcription factor 2.43 L20046 ERCC5/XP-G DNA repair (NER) 2.42 AF060865 ZNF205 Transcription factor 2.42 AL050374 DKFZP586C1619 Unknown 2.42 AF038176 FLJ11193 Hypothetical protein 2.41 AB011152 CENTD1 Unknown 2.41 X51345 JUNB Transcription factor (oncogenesis) 2.4 X85750 MMD Unknown 2.4 X76732 NUCB2 DNA binding activity 2.38 AI827895 FLJ38383 EST 2.38 U43374 MGC21654 Unknown 2.38 X95152 BRCA2 DNA repair 2.37 AL049940 RYBP Transcriptional repressor 2.37 D12614 TNFSF1 (LTA) Pro-apoptotic 2.36 L08895 MEF2C Transcriptional regulator 2.36 AB002306 KIAA0308 Unknown 2.36 X02761 Fibronectin1 Structural 2.35 Y16522 STOML1 Unknown 2.35 L75847 ZNF45 Transcription factor (development) 2.35 U72649 BTG 2 Growth suppression 2.35 U77949 CDC6 Cell cycle control 2.35 L38933 GT198 co activator 2.34 AB014569 KIAA0669 Unknown 2.34 AJ236885 ZNF148 Transcriptional repressor 2.33 AF038951 ZNF253 Transcriptional regulator 2.32 AI337192 SH3BGR Protein complex assembling 2.32 D63482 GIT2 Signal transduction 2.32 M59465 TNFAIP3 Signaling (anti-apoptotic) 2.32 U57627 OCRL Lipid metabolism 2.31 AL049471 DKFZp586N012 Unknown 2.31 U26710 CBLB Signal transduction 2.31 AF052224 BSN Cytoskeleton 2.3 AB022718 DEPP Unknown 2.3 X92841 MICA Stress response 2.3

DOWN-REGULATED AL009179 H2A Histone -2.32 GENES AI087268 SNRPC Small ribonucleoprotein -2.32

12

AA121509 LSM7 Small ribonucleoprotein -2.33 AA121509 LSM7 Small ribonucleoprotein -2.35 X60382 COL10A1 Structural -2.35 AW024285 FLJ12443 Hypothetical protein -2.35 AB020713 Nucleoporin210 Nuclear pore complex -2.35 M12792 CYP21 Lipid metabolism -2.39 W28493 HSPA8 Heat shock protein -2.4 AL050396 Filamin A Cell shape -2.44 AJ004832 NTE Serine -2.47 U29344 FASN Lipid metabolism -2.47 D63481 SCRIB Potential tumor suppressor -2.8 U94888 CCBP2 Signaling (tumorigenesis) -2.85 U19142 GAGE1 Tumor antigen -3.23 D49490 PDIR Protein folding -3.47 X91117 SLC6A2 Small molecule transporter -3.49 AF053356 GNB2 Signal transduction -12.77

1 Derived from LocusLink and SwissProt databases or recent publications in case of incomplete annotations 2 Signal of MNNG-treated WTK1 cells vs untreated WTK1 cells. The fold change threshold was raised to 2.3, since the experiments for this cell line were carried out without biological replicates

13

Supplementary material, Table D. A complete list of genes that were up- or down-regulated in 293T Lαcells upon MNNG treatment independently of the hMLH1expression

Gene Bank Title Category Function Time point(s) 2 Access no Up-regulated V01512 c-fos Signal transduction Growth and apoptosis control 12,30,72 J04111 c-jun Signal transduction Growth and apoptosis control 72 U27193 DUSP8 Signal transduction JNK-p38 inactivation 12,30,72 X68277 DUSP1 Signal transduction JNK inactivation 12,30,72 AJ131693 AKAP9 Signal transduction Scaffolding for PKA 72 J03358 FER Signal transduction Kinase 72 AA224832 STK39 (SPAK) Signal transduction Stress response 12 U83981 GADD34 Cell cycle Cell growth and apoptosis 30, 72 L19871 ATF3 Transcription Stress response 12,30,72 U66619 SMARCD3 Transcription Chromatin modeling 12,30,72 AB007931 Rb-assoc factor 600 Transcription Zn finger 12,30,72 S78296 INA Cytoskeleton Intermediate filament 12,30,72 M13452 lamin A Cytoskeleton Cell shape 12,30,72 X95735 zyxin Cytoskeleton Cell adhesion 12,30,72 Y08683 CPT1B Metabolism Fatty acids oxidation 30, 72 D55649 MAN2A2 Metabolism Mannosidase 12,30,72 AA669799 ASMTL Metabolism Acetylserotonin methyltransferase-like 12,30,72 D13642 SF3b RNA binding protein Splicing factor 12,30,72 AL049219 RBM8A RNA binding protein Not known 12, 72 U06632 coilin RNA binding protein Nuclear protein 12 D64108 DMC1 DNA repair Recombination 30, 72 AA255502 H4FG Histone Nucleasome 12 AA099265 RECK Extracellular matrix Metalloprotease inhibitor 12, 72 AL021937 ret finger prot-like antisense Others No protein product 12,30,72 HG2724-HT2820 tls/chop Others Fusion oncogene 30, 72 AB020662 golgin-67 Others Not known 72 X15674 pTR5 Others Repetitive sequence 12, 30 U79274 clone23733 Others Not known 12,30,72 AL021068 LOC57821 Others Not known 12, 30 U88153 PELP1 Others Estrogen rec modulator 12, 72 AB007895 KIAA0435 Others Not known 12,30,72 AB014590 KIAA0690 Others Not known 12 AI880840 KIAA1036 Others 5'nucleotidasse domain 12,30,72 AB029012 KIAA1089 Others Nuclear protein 12 AW016815 HS380430 EST Homology to RNA helicase 12,30,72 AI827895 HS433517 EST Similar to splicing factor 12,30,72

Down-regulated U59305 PK428 Signal transduction Ser/Thr kinase 12,30,72 AI652978 HAN11 Signal transduction Transcptional signals 12,30,72 Y13493 DYRK2 Signal transduction Growth suppression? 12, 30 D88532 PI3K reg sub 3PIK 3R3 Signal transduction Insulin pathway 12,30,72 U01062 ITPR3 Signal transduction Calcium release 12 L27560 IGFBP5 Signal transduction Growth stimulation 12 HG3432-HT3621 FGFR alt spliced Signal transduction FGF receptor 12, 30 Z71929 FGFRec 2 Signal transduction Growth stimulation 12,30,72 U96876 INSIG1 Signal transduction Growth control 12 U05227 RAB40B Signal transduction Ras family 12 HG1879-HT1919 TC10 Signal transduction Ras family 12, 30 U50062 RIPK1 Signal transduction Ser/Thr kinase 12,30,72 M34181 PKA catalitic sub Signal transduction Kinase 12, 30 X76061 Rb-like 2 (p130) Signal transduction Growht control 12, 30 X12534 RAP2A Signal transduction Ras family 12 AF007567 IRS4 Signal transduction Insulin pathways 12,30,72 L22075 GNBP alpha 13 Signal transduction G-protein signaling 12, 30 Z11695 MAPK1 Signal transduction Stress response 12,30,72 L33881 PKC iota Signal transduction Kinase 12, 30 U24153 PAK2 Signal transduction Apoptotic signaling 12,30,72 U03106 p21 Cell cycle Growth suppression 12,30,72 AF023158 CDC14B Cell cycle M-phase regulator 12,30,72 L07648 MXI1 Cell cycle Inhibition of c-myc 12, 30 U72649 BTG2 Cell growth Growth suppression 12, 30 L22475 bax γ Apoptotic signaling Apoptosis? 12,30,72 U19599 bax δ Apoptotic signaling Apoptosis? 12,30,72 U65092 MSG1 Transcription Cbp/p300-interacting 30, 72 AF040963 SMAD4 Transcription Growth suppression 12,30,72 M27691 CREB1 Transcription G-protein signaling 12,30,72 M88163 SMARCA1 Transcription Chromatin modeling 12 AI635895 DSIPI Transcription Transcriptional regulator 12 AJ223352 H2B Histone Nucleasome 12,30,72 AI189287 H1F2 Histone Nucleasome 12 X13839 Vasc. smooth α actin Cytoskeleton Cell shape 12,30,72 L22075 GNBP alpha 13 Cytoskeleton Cell motility 12, 30 Z26317 Desmoglein 2 Cytoskeleton Cell adhesion 12,30,72 L06237 microtubule-assoc prot1B Cytoskeleton Cell shape 30 U37122 Adducin3 Cytoskeleton Structural 12, 30 U13616 Ankirin3 Cytoskeleton Vesicle transport 12 M61733 EPB41 Cytoskeleton Cell shape 12, 30 AF052288 HIP1 Cytoskeleton Cell shape 12 X07834 SOD2 Metabolism Oxidative stress response 12,30,72

14

Z12173 GNS Metabolism glycosamminoglycan metabolism 12,30,72 AF040958 Sialidase 1 Metabolism Lysosomal 12,30,72 AA877795 ATP6V1D Metabolism ATP synthesis 12,30,72 Y13647 SCD Metabolism Fatty acid synthesis 12,30,72 M93405 ALDH6A1 Metabolism Aldehyde dehydrogenase 12,30,72 D78177 QPRT Metabolism Quinolate phosphoribosiltransferase 12 AB020645 Glutaminase Metabolism Glutamine metabolism 12,30,72 AF026947 AKR7A2 Metabolism aldheyde reductase 12,30,72 Z49878 GAMT Metabolism creatine synthesis 12 AF005418 CYP26A1 Metabolism Energy metabolism 12 NM001098 Aconitase Metabolism Energy pathways 12, 30 X61094 GM2A Metabolism ganglioside metabolism 12,30,72 S79219 propyonil CoA carboxilase Metabolism Fatty acid metabolism 12 L10347 collagen II alpha1 Extracellular matrix Collagen family 12,30,72 M10905 Fibronectin 1 Extracellular matrix Cell adhesion 12, 30 L13210 Mac-2 bind prot Extracellular matrix Scavenger receptor 12, 30 M61916 Laminin β1 Extracellular matrix Basement membrane prot 12, 30 M58526 collagen IV alpha 5 Extracellular matrix Collagen family 12, 30 X15998 Chondroitin sulf proteoglycan II Extracellular matrix Cell adhesion 12 D21337 collagen IV alpha 6 Extracellular matrix Collagen family 12 L35251 MFAP3 Extracellular matrix Microfibillar-associated 12,30,72 AF059274 CSPG5 Membrane fraction Proteoglycan 12,30,72 X77196 LAMP2 Membrane fraction Lysosomal 12,30,72 M11507 Tranferrin receptor Membrane fraction Iron metabolism 12,30,72 L00352 LDL receptor Membrane fraction Lipid metabolism 12,30,72 M82809 AnnexinIV Membrane fraction A2 inhibitor 12, 30 AF019612 MBTPS2 Membrane fraction Transcptional regulator/lipid met. 12,30,72 X52947 gap junction protein alpha 1 Membrane fraction Ion transporter 12, 30 U50410 Glypican3 Membrane fraction Growth control ? 12, 30 AB018286 neurexin3 Membrane fraction Cell adhesion 12,30,72 AB007935 IGSF3 Membrane fraction Ig domain 12, 30 U19878 TMEFF1 Membrane fraction EGF-like domains 12 AF023676 TM7SF2 Membrane fraction laminin beta receptor like 12,30,72 AL049257 BCMP1 Membrane fraction Not known 12 Z48579 ADAM 10 Membrane fraction Cell-cell signaling 12,30,72 AB020316 uronyl 2 sulfotransferase Membrane fraction Protein sulfatation 12, 30 U59321 p72 DDX1 RNA binding protein Splicing factor 12,30,72 AL009266 RBM9 RNA binding protein fox-1 (RNA binding prot) 12,30,72 U05237 Fetal alzheimer ag Development Neurogenesis 12, 30 X59841 PBX3 Development Transcription factor 12,30,72 X98834 SALL2 Development Transcription factor 12 Z81326 neuroserpin Development Proteinase inhibitor 12,30,72 AB009285 CFDP1 Development Craniofacial development 12,30,72 D29990 SLC7A2 Transporter Cationin aminoacid transporter 12,30,72 U07139 CACNB3 Transporter Calcium channel 12 M10098 18S rRNA Others Ribosomal RNA 12 X95677 ARGBPIB Others Arginine function regulator 12,30,72 M15518 t-PA Others Coagulation 12,30,72 U16954 ALL fused gene Others Expressed in leukemia 12 U58516 milk fat globule prot Others Cell adhesion 12 U29091 SELENBP1 Others Selenium binding 12 D83077 TTC3 Others Down syndrome pathogenesis 12 X80507 YAP1 Others Protein binding 12,30,72 J03589 ubiquitin-like 4 Others Proteasome 12 AB002364 ADAMTS3 Others Procollagene peptidase 12 U02390 CAP2 Others Not known 12 AF004849 HIPK3 Others Kinase 12,30,72 AL050389 DKFZP564F013 Others Hypotetical protein 12,30,72 M55536 Glucose transporter pseudogene Others No product 12,30,72 AL050159 DKFZP586A0522 Others Hypothetical protein 12, 30 AL050141 FLJ20719 Others Hypothetical protein 12,30,72 Z85986 DKFZp451N147 Others Hypothetical protein 12,30,72 AL021396 DJ971N18.2 Others Hypothetical protein 12, 30 AF052107 clone 23620 EST 12

1 Derived from LocusLink and SwissProt databases or recent publications in case of incomplete annotations 2 Time point at which the multiple regression analysis showed a significant (p<0.05) up- or down-regulation of the gene upon MNNG-treatment independently of the presence of hMLH1 (in both 293T Lα+ and Lα- cells)

15