Dr. Denis Wirtz Professor of Chemical and Biomolecular Engineering, Vice Provost for Research Johns Hopkins University

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Dr. Denis Wirtz Professor of Chemical and Biomolecular Engineering, Vice Provost for Research Johns Hopkins University Biography In his role as vice provost for research, Dr. Wirtz focuses on the current and future health of the university’s research enterprise, including institutional research compliance, research administration and development, and cross-divisional research initiatives, such as the Bloomberg Distinguished Professorships, the Johns Hopkins Catalyst and Discovery Awards, and the President’s Frontier Awards. A Johns Hopkins faculty member since 1994, Wirtz studies the biophysical properties of healthy and diseased cells, including interactions between adjacent cells and the role of cellular architecture on nuclear shape and gene expression. He holds the T.H. Smoot Professorship in the Whiting School of Engineering with secondary appointments in the School of Medicine. He has authored more than 200 peer-reviewed articles, and his research has been cited more than 14,000 times. Abstract “Cancer cell biology in 3D “ Two-dimensional (2D) in vitro culture systems have for a number of years provided a controlled and versatile environment for mechanistic studies of cell adhesion, polarization, and migration, three interrelated cell functions critical to cancer metastasis. However, the organization and functions of focal adhesion proteins, protrusion machinery, and microtubule-based polarization in cells embedded in physiologically more relevant 3D extracellular matrices is qualitatively different from their organization and functions on conventional 2D planar substrates. This talk will describe the implications of the dependence of focal adhesion protein-based cell migration on micro-environmental dimensionality (1D vs. 2D vs. 3D), how cell micromechanics plays a critical role in promoting local cell invasion, and associated validation in mouse models. We will also discuss the molecular and biophysical mechanisms used by cancer cells to negotiate different matrix microstructures. Finally, we will discuss the implications of this work in metastatic cancer. .

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Gilkes JBC 2013.Pdf
Glycobiology and Extracellular Matrices: Hypoxia-inducible Factor 1 (HIF-1) Promotes Extracellular Matrix Remodeling under Hypoxic Conditions by Inducing P4HA1, P4HA2, and PLOD2 Expression in Fibroblasts Daniele M. Gilkes, Saumendra Bajpai, Pallavi Chaturvedi, Denis Wirtz and Gregg L. Semenza J. Biol. Chem. 2013, 288:10819-10829. doi: 10.1074/jbc.M112.442939 originally published online February 19, 2013 Access the most updated version of this article at doi: 10.1074/jbc.M112.442939 Find articles, minireviews, Reflections and Classics on similar topics on the JBC Affinity Sites. Alerts: • When this article is cited • When a correction for this article is posted Click here to choose from all of JBC's e-mail alerts Supplemental material: http://www.jbc.org/content/suppl/2013/02/21/M112.442939.DC1.html This article cites 53 references, 21 of which can be accessed free at http://www.jbc.org/content/288/15/10819.full.html#ref-list-1 Downloaded from http://www.jbc.org/ at JOHNS HOPKINS UNIVERSITY on October 12, 2013 THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 15, pp. 10819–10829, April 12, 2013 © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A. Hypoxia-inducible Factor 1 (HIF-1) Promotes Extracellular Matrix Remodeling under Hypoxic Conditions by Inducing P4HA1, P4HA2, and PLOD2 Expression in Fibroblasts*□S Received for publication, December 6, 2012, and in revised form, January 17, 2013 Published, JBC Papers in Press, February 19, 2013, DOI 10.1074/jbc.M112.442939 Daniele M. Gilkes‡§¶1, Saumendra Bajpai¶ʈ, Pallavi Chaturvedi‡§, Denis Wirtz¶ʈ, and Gregg L.
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70380048.Pdf
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bioRxiv preprint doi: https://doi.org/10.1101/2020.12.08.416909; this version posted December 9, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. In situ characterization of the 3D microanatomy of the pancreas and pancreatic cancer at single cell resolution Ashley Kiemen1, Alicia M. Braxton2, Mia P. Grahn1, Kyu Sang Han1, Jaanvi Mahesh Babu2, Rebecca Reichel2, Falone Amoa2, Seung-Mo Hong3, Toby C. Cornish4, Elizabeth D. Thompson2, Laura D. Wood2, Ralph H. Hruban2, Pei-Hsun Wu1*, Denis Wirtz1,2,6,7* 1 Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA 2 Department of Pathology, The Sol Goldman Pancreatic Cancer Research Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA 3 Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea 4 Department of Pathology, University of Colorado School of Medicine, Aurora, Colorado 80045, USA 5 Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA 6 Department of Materials Science and Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA 7 Johns Hopkins Physical Sciences - Oncology Center, The Johns Hopkins University, Baltimore, Maryland 21218, USA *Corresponding authors: Denis Wirtz ([email protected]) and Pei-Hsun Wu ([email protected]) Keywords: digital pathology, deep learning, pancreatic cancer, cancer invasion bioRxiv preprint doi: https://doi.org/10.1101/2020.12.08.416909; this version posted December 9, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder.
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