Product datasheet Anti-P2RX5 Antibody Catalog Number: PB0339

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-P2RX5 Antibody Name P2RX5 Source Rabbit IgG Species Reactivity mouse,rat,human Tested Application WB,IHC-P Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human P2X5 recombinant (Position: D333-T422). Human P2X5 shares 42% amino acid (aa) sequence identity with rat P2X5. Purification Immunogen affinity purified. Observed MW 47KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry(Paraffin-embedded Section): 1:50-400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information P2X purinoceptor 5 is a protein that in humans is encoded by the P2RX5 gene. The product of this gene belongs to the family of purinoceptors for ATP. It has been identified that the P2RX5 gene within a BAC contig covering 17p13. This functions as a -gated . Several characteristic motifs of ATP-gated channels are present in its primary structure, but, unlike other members of the purinoceptors family, this receptor has only a single transmembrane domain. Quantitative dot blots revealed that P2X5R expression is highest in brain and immune system and is developmentally regulated.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-P2RX5 Antibody Catalog Number: PB0339

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. IHC analysis of analysis of P2X5 using P2X5 using anti-P2X5 anti-P2X5 antibody antibody (PB0339).P2X5 (PB0339). Electrophoresis was detected in paraffin- was performed on a embedded section of 5-20% SDS-PAGE gel at Mouse Brain Tissue. Heat 70V (Stacking gel) / 90V mediated antigen (Resolving gel) for 2-3 retrieval was performed hours. The sample well of in citrate buffer (pH6, each lane was loaded epitope retrieval solution) with 50ug of sample for 20 mins. The tissue under reducing section was blocked with conditions. Lane 1: Rat 10% goat serum. The Brain Tissue Lysate. After tissue section was then Electrophoresis, incubated with 1μg/ml were transferred to a rabbit anti-P2X5 Nitrocellulose membrane Antibody (PB0339) at 150mA for 50-90 overnight at 4°C. minutes. Blocked the Biotinylated goat anti- membrane with 5% Non- rabbit IgG was used as fat Milk/ TBS for 1.5 hour secondary antibody and at RT. The membrane was incubated for 30 minutes incubated with rabbit anti- at 37°C. The tissue P2X5 antigen affinity section was developed purified polyclonal using Strepavidin-Biotin- antibody (Catalog # Complex (SABC)(Catalog PB0339) at 0.5 μg/mL # SA1022) with DAB as overnight at 4°C, then the chromogen. washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2X5 at approximately 47KD. The expected band size for P2X5 is at 47KD.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-P2RX5 Antibody Catalog Number: PB0339

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Figure 3. IHC analysis of P2X5 using anti-P2X5 antibody (PB0339).P2X5 was detected in paraffin- embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-P2X5 Antibody (PB0339) overnight at 4°C. Biotinylated goat anti- rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin- Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3

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