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NIS-Elements Documentation User's Guide (Ver. 4.00)

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NIS-Elements Documentation User's Guide (Ver. 4.00) M396E 11.9.NF.17 *M396EN17* NIS-Elements Documentation User’s Guide (Ver. 4.00) Thank you very much for choosing Nikon. This manual explains installation and use of the NIS-Elements Documentation. For trouble-free operation, read this manual before using the program. No part of this manual may be reproduced and transmitted without Nikon’s permission. The content of this manual is subject to change without notice. Nikon has carefully prepared this manual. However, we make no expressed or implied warranty of any kind and assume no responsibility for such errors or omissions. Be sure to read the instruction manuals for the microscope and PC you plan to use with the NIS-Elements Documentation. Trademarks: Microsoft® and Windows® are either registered trademarks or trademarks of Microsoft Corporation in the United States or other countries. Products and brand names are trademarks or registered trademarks of their respective companies. The “TM” and ®marks are not used to identify registered trademarks and trademarks in this manual. To run NIS-Elements Documentation optimally, the following specifications are required. Minimum PC Specification: CPU: Intel Core 2 Duo or higher RAM for 32bit OS: 2GB or higher RAM for 64bit OS: 4GB or higher Operating system: Windows 7 Professional SP1 32bit/64bit (English/Chinese) HDD: 2GB or more of available hard disk space for installation Display: 1280x1024 pixel (True Color mode) User rights: Administrators for installation * Operation cannot be guaranteed on all computer models. For further information, contact your nearest Nikon representatives. NIS-Elements D Table of Contents 1. Installation and Startup .......................................................................................................... 1 1.1. NIS-Elements D Installation ........................................................................................... 1 1.2. Command Line Options ................................................................................................ 6 1.2.1. Syntax and Example: ......................................................................................... 6 1.2.2. Switches: ....................................................................................................... 6 2. Application Appearance .......................................................................................................... 9 2.1. NIS-Elements D Screen ................................................................................................ 9 2.1.1. Menu Bar ....................................................................................................... 9 2.1.2. Application Tool Bars ....................................................................................... 11 2.1.3. Status Bar .................................................................................................... 11 2.1.4. Image Window ............................................................................................... 11 2.2. Modifying Layout ...................................................................................................... 13 2.2.1. Layouts ....................................................................................................... 13 2.2.2. Layout Manager ............................................................................................. 14 2.2.3. Docking Panes .............................................................................................. 19 2.2.4. Appearance Options ....................................................................................... 21 2.3. Organizer ............................................................................................................... 23 2.3.1. Image Filter .................................................................................................. 23 2.3.2. Operations with Images ................................................................................... 25 2.3.3. Adjusting the Organizer Layout ........................................................................... 26 2.4. User Management .................................................................................................... 26 3. Setting the Hardware Up ....................................................................................................... 31 3.1. Working with a Camera .............................................................................................. 31 3.1.1. Live Signal from Camera .................................................................................. 32 3.1.2. Capturing Images ........................................................................................... 32 3.1.3. Camera ROI on Live Image ................................................................................ 32 3.1.4. Controlling Cameras by Macro ........................................................................... 33 3.2. Camera Settings ...................................................................................................... 33 3.3. Connected Devices ................................................................................................... 36 3.3.1. Logical Devices .............................................................................................. 37 3.3.2. Tips ............................................................................................................ 39 3.4. Optical Configurations ................................................................................................ 39 3.5. Objectives .............................................................................................................. 42 3.5.1. Managing Objectives ....................................................................................... 42 3.5.2. Objective Calibration ....................................................................................... 43 3.5.3. Units .......................................................................................................... 46 4. Capturing .......................................................................................................................... 47 4.1. Single Image Capture ................................................................................................ 47 4.2. ND2 Acquisition ....................................................................................................... 47 4.2.1. Time-lapse Acquisition ..................................................................................... 48 4.2.2. Multi-point Acquisition ..................................................................................... 49 4.2.3. Z-series Acquisition ......................................................................................... 51 4.3. Capturing movies ..................................................................................................... 53 5. Image Files ........................................................................................................................ 55 5.1. Composition ............................................................................................................ 55 5.1.1. Image Layers ................................................................................................ 55 5.1.2. Image Types in NIS-Elements D ......................................................................... 56 5.1.3. nd2 files ...................................................................................................... 56 5.2. Working with Image Files ............................................................................................ 59 5.2.1. Opening Saved Images .................................................................................... 59 5.2.2. Switching Between Loaded Images ..................................................................... 60 5.2.3. Closing Images .............................................................................................. 60 5.2.4. Saving Images with UAC ................................................................................... 60 5.2.5. Copying Channels by Drag and Drop .................................................................... 60 5.2.6. Image Formats .............................................................................................. 61 6. Image Analysis ................................................................................................................... 63 6.1. Image Processing ..................................................................................................... 63 6.1.1. Processing On: Intensity/RGB/Channels ............................................................... 63 6.2. Histogram and Look Up Tables ..................................................................................... 63 6.2.1. Histogram .................................................................................................... 63 6.2.2. LUTs ........................................................................................................... 65 6.3. Thresholding ........................................................................................................... 71 6.3.1. RGB Mode .................................................................................................... 71 6.3.2. HSI Mode ..................................................................................................... 73 6.3.3. Intensity Mode .............................................................................................
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