CONTENTS • DECEMBER 2020 • VOLUME 94, NO. 24

COVER IMAGE Cover photograph: Cryo-electron microscopy structure of human picobirnavirus (hPBV) at 2.6-Å resolution. hPBV is a double-stranded RNA virus which is broadly extended in the human population, but its pathogenicity is uncertain. The hPBV capsid is built of 60 quasisymmetric capsid protein dimers (blue and yellow) stabilized by domain swapping. Using an in vitro reversible assembly/disassembly system of hPBV, dimers and tetramers are isolated as possible assembly intermediates. (See related article at e01542-20.) (Copyright © 2020 American Society for Microbiology. All Rights Reserved.)

EDITORIALS Stacey R Recognizing the Top 25 Peer Reviewers for the Journal of Virology e01941-20 Schultz-Cherry Editorial Rozanne M. Sandri-Goldin Stacey R Acknowledgment of Ad Hoc Reviewers e01942-20 Schultz-Cherry Editorial Rozanne M. Sandri-Goldin

SPOTLIGHT Rozanne M. R Articles of Significant Interest in This Issue e02018-20 Sandri-Goldin Spotlight

STRUCTURE AND ASSEMBLY Richard M. T Role of the Herpes Simplex Virus CVSC Proteins at the Capsid Portal Vertex e01534-20 Longnecker Structure Alexis Huet, Jamie B. Huffman, James F. Conway, Fred L. Homa Susana Lopez T Cryo-electron Microscopy Structure, Assembly, and Mechanics Show e01542-20 Structure Morphogenesis and Evolution of Human Picobirnavirus Álvaro Ortega-Esteban, Carlos P. Mata, María J. Rodríguez-Espinosa, Daniel Luque, Nerea Irigoyen, Javier M. Rodríguez, Pedro J. de Pablo, José R. Castón

GENOME REPLICATION AND REGULATION OF VIRAL GENE EXPRESSION Rebecca Ellis DutchT Using SHAPE-MaP To Model RNA Secondary Structure and Identify 3=UTR e00701-20 Replication Variation in Chikungunya Virus Emily A. Madden, Kenneth S. Plante, Clayton R. Morrison, Katrina M. Kutchko, Wes Sanders, Kristin M. Long, Sharon Taft-Benz, Marta C. Cruz Cisneros, Ashlyn Morgan White, Sanjay Sarkar, Grace Reynolds, Heather A. Vincent, Alain Laederach, Nathanial J. Moorman, Mark T. Heise

GENETIC DIVERSITY AND EVOLUTION Colin R. Parrish T Tissue Tropisms of Avian Influenza A Viruses Affect Their Spillovers from Wild e00847-20 Diversity & Evol Birds to Pigs Xiaojian Zhang, Fred L. Cunningham, Lei Li, Katie Hanson-Dorr, Liyuan Liu, Kaitlyn Waters, Minhui Guan, Alicia K. Olivier, Brandon S. Schmit, Jacqueline M. Nolting, Andrew S. Bowman, Mia Kim Torchetti, Thomas J. DeLiberto, Xiu-Feng Wan Joanna L. Shisler T Structure-Based Deep Mining Reveals First-Time Annotations for 46 Percent of e00854-20 Diversity & Evol the Dark Annotation Space of the 9,671-Member Superproteome of the Nucleocytoplasmic Large DNA Viruses Yeva Mirzakhanyan, Paul David Gershon

Instructions to Authors are available on the journal website. CONTENTS • DECEMBER 2020 • VOLUME 94, NO. 24

Lawrence Banks T Comprehensive Assessment of the Antigenic Impact of Human Papillomavirus e01236-20 Diversity & Evol Lineage Variation on Recognition by Neutralizing Monoclonal Antibodies Raised against Lineage A Major Capsid Proteins of Vaccine-Related Genotypes Anna Godi, Dolcibella Boampong, Busayo Elegunde, Kavita Panwar, Maxime Fleury, Shaowei Li, Qinjian Zhao, Ningshao Xia, Neil D. Christensen, Simon Beddows Colin R. Parrish T A Heterogeneous Swine Show Circuit Drives Zoonotic Transmission of Influenza e01453-20 Diversity & Evol A Viruses in the United States Martha I. Nelson, Amanda Perofsky, Dillon S. McBride, Benjamin L. Rambo-Martin, Malania M. Wilson, John R. Barnes, Harm van Bakel, Zenab Khan, Jayeeta Dutta, Jacqueline M. Nolting, Andrew S. Bowman Colin R. Parrish T Two Sides of a Coin: a Zika Virus Mutation Selected in Pregnant Rhesus e01605-20 Diversity & Evol Macaques Promotes Fetal Infection in Mice but at a Cost of Reduced Fitness in Nonpregnant Macaques and Diminished Transmissibility by Vectors Danilo Lemos, Jackson B. Stuart, William Louie, Anil Singapuri, Ana L. Ramírez, Jennifer Watanabe, Jodie Usachenko, Rebekah I. Keesler, Claudia Sanchez-San Martin, Tony Li, Calla Martyn, Glenn Oliveira, Sharada Saraf, Nathan D. Grubaugh, Kristian G. Andersen, James Thissen, Jonathan Allen, Monica Borucki, Konstantin A. Tsetsarkin, Alexander G. Pletnev, Charles Y. Chiu, Koen K. A. Van Rompay, Lark L. Coffey

VIRUS-CELL INTERACTIONS Richard M. T De Novo Cholesterol Biosynthesis and Its Trafficking in LAMP-1-Positive Vesicles e01001-20 Longnecker Interactions Are Involved in Replication and Spread of Marek’s Disease Virus Nitish Boodhoo, Nitin Kamble, Shahriar Behboudi Rebecca Ellis DutchT Primary Swine Respiratory Epithelial Cell Lines for the Efficient Isolation and e01091-20 Interactions Propagation of Influenza A Viruses Victoria Meliopoulos, Sean Cherry, Nicholas Wohlgemuth, Rebekah Honce, Karen Barnard, Phillip Gauger, Todd Davis, Peter Shult, Colin Parrish, Stacey Schultz-Cherry Frank Kirchhoff T Potential for Virus Endogenization in Humans through Testicular Germ Cell e01145-20 Interactions Infection: the Case of HIV Dominique Mahé, Giulia Matusali, Claire Deleage, Raquel L. L. S. Alvarenga, Anne-Pascale Satie, Amélie Pagliuzza, Romain Mathieu, Sylvain Lavoué, Bernard Jégou, Luiz R. de França, Nicolas Chomont, Laurent Houzet, Antoine D. Rolland, Nathalie Dejucq-Rainsford Rebecca Ellis DutchT Cytoplasmic Cargo Receptor p62 Inhibits Avibirnavirus Replication by Mediating e01255-20 Interactions Autophagic Degradation of Viral Protein VP2 Yahui Li, Boli Hu, Gang Ji, Yina Zhang, Chenyang Xu, Jing Lei, Chan Ding, Jiyong Zhou Tom Gallagher T Interferon-Induced Transmembrane Protein 3 Is a Virus-Associated Protein Which e01350-20 Interactions Suppresses Porcine Reproductive and Respiratory Syndrome Virus Replication by Blocking Viral Membrane Fusion Angke Zhang, Hong Duan, Huijun Zhao, Huancheng Liao, Yongkun Du, Liangliang Li, Dawei Jiang, Bo Wan, Yanan Wu, Pengchao Ji, En-Min Zhou, Gaiping Zhang Richard M. T Epstein-Barr Virus Episome Physically Interacts with Active Regions of the Host e01390-20 Longnecker Interactions Genome in Lymphoblastoid Cells Luopin Wang, Jun Laing, Bingyu Yan, Hufeng Zhou, Liangru Ke, Chong Wang, Yohei Narita, Zonghao Zhang, Matthew R. Olson, Behdad Afzali, Bo Zhao, Majid Kazemian

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Tom Gallagher T Chikungunya Virus Strains from Each Genetic Clade Bind Sulfated e01500-20 Interactions Glycosaminoglycans as Attachment Factors Nicole McAllister, Yan Liu, Lisete M. Silva, Anthony J. Lentscher, Wengang Chai, Nian Wu, Kira A. Griswold, Krishnan Raghunathan, Lo Vang, Jeff Alexander, Kelly L. Warfield, Michael S. Diamond, Ten Feizi, Laurie A. Silva, Terence S. Dermody Bryan R. G. T Dengue Virus Targets Nrf2 for NS2B3-Mediated Degradation Leading to e01551-20 Williams Interactions Enhanced Oxidative Stress and Viral Replication Matteo Ferrari, Alessandra Zevini, Enrico Palermo, Michela Muscolini, Magdalini Alexandridi, Marilena P. Etna, Eliana M. Coccia, Ana Fernandez-Sesma, Carolyn Coyne, Donna D. Zhang, Ernesto T. A. Marques, David Olagnier, John Hiscott Joanna L. Shisler T White Spot Syndrome Virus Benefits from Endosomal Trafficking, Substantially e01570-20 Interactions Facilitated by a Valosin-Containing Protein, To Escape Autophagic Elimination and Propagate in the Crustacean Cherax quadricarinatus Chuang Meng, Ling-Ke Liu, Dong-Li Li, Rui-Lin Gao, Wei-Wei Fan, Ke-Jian Wang, Han-Ching Wang, Hai-Peng Liu Rozanne M. T Role of Phosphatidylethanolamine Biosynthesis in Herpes Simplex Virus 1-Infected e01572-20 Sandri-Goldin Interactions Cells in Progeny Virus Morphogenesis in the Cytoplasm and in Viral Pathogenicity In Vivo Jun Arii, Ayano Fukui, Yuta Shimanaka, Nozomu Kono, Hiroyuki Arai, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasuko Mori, Yasushi Kawaguchi Stacey T OASL Triggered by Novel Goose Astrovirus via ORF2 Restricts Its Replication e01767-20 Schultz-Cherry Interactions Dan Ren, Tuofan Li, Xinyu Zhang, Xiaohui Yao, Wei Gao, Quan Xie, Jianjun Zhang, Hongxia Shao, Zhimin Wan, Aijian Qin, Jianqiang Ye Rozanne M. T Herpes Simplex Virus Entry by a Nonconventional Endocytic Pathway e01910-20 Sandri-Goldin Interactions Giulia Tebaldi, Suzanne M. Pritchard, Anthony V. Nicola

CELLULAR RESPONSE TO INFECTION Bryan R.G. Williams T The Epigenetic Regulator EZH2 Instructs CD4 T Cell Response to Acute Viral e01627-20 Response Infection via Coupling of Cell Expansion and Metabolic Fitness Ren Li, Zhiwei Pan, Jialin Wu, Shuai Yue, Yao Lin, Yang Yang, Zhirong Li, Li Hu, Jianfang Tang, Lingling Shan, Qin Tian, Peng Jiang, Ping Wei, Lilin Ye, Pinghuang Liu, Xiangyu Chen Colin R. Parrish T The Nucleoprotein of H7N9 Influenza Virus Positively Regulates TRAF3-Mediated e01640-20 Response Innate Signaling and Attenuates Viral Virulence in Mice Yanli Wei, Yan Zeng, Xuegang Zhang, Shuai Xu, Zhengxiang Wang, Yingying Du, Bo Zhang, Cao-Qi Lei, Qiyun Zhu

VACCINES AND ANTIVIRAL AGENTS Colin R. Parrish T Cross-Protection by Inactivated H5 Prepandemic Vaccine Seed Strains against e00720-20 Vaccines Diverse Goose/Guangdong Lineage H5N1 Highly Pathogenic Avian Influenza Viruses Miria Ferreira Criado, Mariana Sá e Silva, Dong-Hun Lee, Carolina Alves de Lima Salge, Erica Spackman, Ruben Donis, Xiu-Feng Wan, David E. Swayne Frank Kirchhoff T Rectal Acquisition of Simian Immunodeficiency Virus (SIV) SIVmac239 Infection e00979-20 Vaccines despite Vaccine-Induced Immune Responses against the Entire SIV Proteome Mauricio A. Martins, Lucas Gonzalez-Nieto, Michael J. Ricciardi, Varian K. Bailey, Christine M. Dang, Georg F. Bischof, Nuria Pedreño-Lopez, Matthias G. Pauthner, Dennis R. Burton, Christopher L. Parks, Patricia Earl, Bernard Moss, Eva G. Rakasz, Jeffrey D. Lifson, Ronald C. Desrosiers, David I. Watkins

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Richard M. T Herpes Simplex Virus 1 (HSV-1) 0⌬NLS Live-Attenuated Vaccine Protects against e01000-20 Longnecker Vaccines Ocular HSV-1 Infection in the Absence of Neutralizing Antibody in HSV-1 gB T Cell Receptor-Specific Transgenic Mice Grzegorz B. Gmyrek, Adrian Filiberti, Micaela Montgomery, Alisha Chitrakar, Derek J. Royer, Daniel J. J. Carr Mark T. Heise T Two RNA Tunnel Inhibitors Bind in Highly Conserved Sites in Dengue Virus NS5 e01130-20 Vaccines Polymerase: Structural and Functional Studies Rishi Arora, Chong Wai Liew, Tingjin Sherryl Soh, Dorcas Adobea Otoo, Cheah Chen Seh, Kimberley Yue, Shahul Nilar, Gang Wang, Fumiaki Yokokawa, Christian G. Noble, Yen Liang Chen, Pei-Yong Shi, Julien Lescar, Thomas M. Smith, Timothy E. Benson, Siew Pheng Lim Tom Gallagher T Cross-Protection against MERS-CoV by Prime-Boost Vaccination Using Viral Spike e01176-20 Vaccines DNA and Protein Jung-ah Choi, Junghyun Goo, Eunji Yang, Dae-Im Jung, Sena Lee, Semi Rho, Yuji Jeong, Young-Shin Park, Hayan Park, Young-hye Moon, Uni Park, Sang-Hwan Seo, Hyeja Lee, Jae Myun Lee, Nam-Hyuk Cho, Manki Song, Jae-Ouk Kim Guido Silvestri T Neutralizing Antibody Responses Induced by HIV-1 Envelope Glycoprotein SOSIP e01214-20 Vaccines Trimers Derived from Elite Neutralizers Anna Schorcht, Tom L. G. M. van den Kerkhof, Christopher A. Cottrell, Joel D. Allen, Jonathan L. Torres, Anna-Janina Behrens, Edith E. Schermer, Judith A. Burger, Steven W. de Taeye, Alba Torrents de la Peña, Ilja Bontjer, Stephanie Gumbs, Gabriel Ozorowski, Celia C. LaBranche, Natalia de Val, Anila Yasmeen, Per Johan Klasse, David C. Montefiori, John P. Moore, Hanneke Schuitemaker, Max Crispin, Marit J. van Gils, Andrew B. Ward, Rogier W. Sanders Guido Silvestri T A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts e01225-20 Vaccines Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Thomas Musich, Vishal Thovarai, David J. Venzon, Venkatramanan Mohanram, Iskra Tuero, Leia K. Miller-Novak, Sabrina Helmold Hait, Mohammad Arif Rahman, Ruth Hunegnaw, Erin Huiting, Wuxing Yuan, Colm O’hUigin, Tanya Hoang, Yongjun Sui, Celia LaBranche, David Montefiori, Jenifer Bear, Margherita Rosati, Massimiliano Bissa, Jay A. Berzofsky, George N. Pavlakis, Barbara K. Felber, Genoveffa Franchini, Marjorie Robert-Guroff Felicia Goodrum T Identification and Characterization of CD4؉ T Cell Epitopes after Shingrix Vaccination e01641-20 Vaccines Hannah Voic, Rory D. de Vries, John Sidney, Paul Rubiro, Erin Moore, Elizabeth Phillips, Simon Mallal, Brittany Schwan, Daniela Weiskopf, Alessandro Sette, Alba Grifoni Rebecca Ellis DutchT Stable Attenuation of Human Respiratory Syncytial Virus for Live Vaccines by e01831-20 Vaccines Deletion and Insertion of Amino Acids in the Hinge Region between the mRNA Capping and Methyltransferase Domains of the Large Polymerase Protein Miaoge Xue, Rongzhang Wang, Olivia Harder, Phylip Chen, Mijia Lu, Hui Cai, Anzhong Li, Xueya Liang, Ryan Jennings, Krista La Perle, Stefan Niewiesk, Mark E. Peeples, Jianrong Li

PATHOGENESIS AND IMMUNITY Tom Gallagher T Single-Cell RNA Sequencing Reveals the Diversity of the Immunological e01295-20 Pathogenesis Landscape following Central Nervous System Infection by a Murine Coronavirus Amber R. Syage, H. Atakan Ekiz, Dominic D. Skinner, Colleen Stone, Ryan M. O’Connell, Thomas E. Lane

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Jae U. Jung T Interferon Regulatory Factor 7 Attenuates Chronic Gammaherpesvirus Infection e01554-20 Pathogenesis K. E. Johnson, C. A. Aurubin, C. N. Jondle, P. T. Lange, V. L. Tarakanova Frank Kirchhoff T Genetic Control of Neonatal Immune Tolerance to an Exogenous Retrovirus e01608-20 Pathogenesis Emily Cullum, Stanislav Dikiy, Helen A. Beilinson, Melissa Kane, Alessandra Veinbachs, Vera M. Beilinson, Lisa K. Denzin, Alexander Chervonsky, Tatyana Golovkina Guido Silvestri T HIV Infection and Persistence in Pulmonary Mucosal Double Negative T Cells e01788-20 Pathogenesis In Vivo Oussama Meziane, Syim Salahuddin, Tram N. Q. Pham, Omar Farnos, Amélie Pagliuzza, Ron Olivenstein, Elaine Thomson, Yulia Alexandrova, Marianna Orlova, Erwin Schurr, Petronela Ancuta, Élie Haddad, Nicolas Chomont, Eric A. Cohen, Mohammad-Ali Jenabian, Cecilia T. Costiniuk

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