viruses Article First Diagnosed Case of Camelpox Virus in Israel Oran Erster 1,†, Sharon Melamed 2,†, Nir Paran 2, Shay Weiss 2, Yevgeny Khinich 1, Boris Gelman 1, Aharon Solomony 3 and Orly Laskar-Levy 2,* 1 Division of Virology, Kimron Veterinary Institute, P.O. Box 12, Beit Dagan 50250, Israel;
[email protected] (O.E.);
[email protected] (Y.K.);
[email protected] (B.G) 2 Department of Infectious Diseases, IIBR P.O. Box 19, Ness Ziona 74100, Israel;
[email protected] (S.M.);
[email protected] (N.P.);
[email protected] (S.W.) 3 Negev Veterinary Bureau, Israeli Veterinary Services, Binyamin Ben Asa 1, Be0er Sheba 84102, Israel;
[email protected] * Correspondence:
[email protected]; Tel.: +972-8-938-16051 † These authors contributed equally to this work. Received: 10 January 2018; Accepted: 12 February 2018; Published: 13 February 2018 Abstract: An outbreak of a disease in camels with skin lesions was reported in Israel during 2016. To identify the etiological agent of this illness, we employed a multidisciplinary diagnostic approach. Transmission electron microscopy (TEM) analysis of lesion material revealed the presence of an orthopox-like virus, based on its characteristic brick shape. The virus from the skin lesions successfully infected chorioallantoic membranes and induced cytopathic effect in Vero cells, which were subsequently positively stained by an orthopox-specific antibody. The definite identification of the virus was accomplished by two independent qPCR, one of which was developed in this study, followed by sequencing of several regions of the viral genome. The qPCR and sequencing results confirmed the presence of camelpox virus (CMLV), and indicated that it is different from the previously annotated CMLV sequence available from GenBank.