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Stream Bed Erosion Labs Stream Bed Erosion
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Casomorphins and Gliadorphins Have Diverse Systemic Effects Spanning Gut, Brain and Internal Organs
International Journal of Environmental Research and Public Health Article Casomorphins and Gliadorphins Have Diverse Systemic Effects Spanning Gut, Brain and Internal Organs Keith Bernard Woodford Agri-Food Systems, Lincoln University, Lincoln 7674, New Zealand; [email protected] Abstract: Food-derived opioid peptides include digestive products derived from cereal and dairy diets. If these opioid peptides breach the intestinal barrier, typically linked to permeability and constrained biosynthesis of dipeptidyl peptidase-4 (DPP4), they can attach to opioid receptors. The widespread presence of opioid receptors spanning gut, brain, and internal organs is fundamental to the diverse and systemic effects of food-derived opioids, with effects being evidential across many health conditions. However, manifestation delays following low-intensity long-term exposure create major challenges for clinical trials. Accordingly, it has been easiest to demonstrate causal relationships in digestion-based research where some impacts occur rapidly. Within this environment, the role of the microbiome is evidential but challenging to further elucidate, with microbiome effects ranging across gut-condition indicators and modulators, and potentially as systemic causal factors. Elucidation requires a systemic framework that acknowledges that public-health effects of food- derived opioids are complex with varying genetic susceptibility and confounding factors, together with system-wide interactions and feedbacks. The specific role of the microbiome within -
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Characterisation and Partial Purification of a Novel Prohormone Processing Enzyme from Ovine Adrenal Medulla
Volume 246, number 1,2, 44-48 FEB 06940 March 1989 Characterisation and partial purification of a novel prohormone processing enzyme from ovine adrenal medulla N. Tezapsidis and D.C. Parish Biochemistry Group, School of Biological Sciences, University of Sussex, Falmer, Brighton, Sussex, England Received 3 January 1989 An enzymatic activity has been identified which is capable of generating a product chromatographically identical with adrenorphin from the model substrate BAM 12P. This enzyme was purified by gel filtration and ion-exchange chromatog- raphy and characterised as having a molecular mass between 30 and 45 kDa and an acidic pL The enzyme is active at the acid pH expected in the secretory vesicle interior and is inhibited by EDTA, suggesting that it is a metalloprotease. This activity could not be mimicked by incubation with lysosomal fractions and it meets the criteria to be considered as a possible prohormone processing enzyme. Prohormone processing; Adrenorphin; Secretory vesiclepurification 1. INTRODUCTION The purification of an endopeptidase responsi- ble for the generation of adrenorphin was under- Active peptide hormones are released from their taken, using the ovine adrenal medulla as a source. precursors by endoproteolytic cleavage at highly Adrenorphin is known to be located in the adrenal specific sites. The commonest of these is cleavage medulla of all species so far investigated [6]. at pairs of basic residues such as lysine and Secretory vesicles (also known as chromaffin arginine [1,2]. However another common class of granules) were isolated as a preliminary purifica- processing sites are known to be at single arginine tion step, since it is known that prohormone pro- residues, adjacent to a proline [3]. -
TCPDF Example
Chapter 2 : Gentaur Products List • Ac Leu28 31 Neuropeptide Y 24 36 • Sar1 Ala8 Angiotensin II Gly Ser Arg Ile Asp Arg Ile Gly Ala Gln Ser Gly Met Gly Cys • Gln18 Platelet Factor 4 15 22 human • Sar1 Gly8 Angiotensin II Gly Arg Arg Phe MW 2561 87 Disulfide bridge Cys4 Cys20 • Platelet Factor 4 58 70 human AA Pro Leu Tyr Lys Lys Ile • Sar1 Thr8 Angiotensin II MW 2561 87 Ile Lys Lys Leu Leu Glu Ser MW 1573 01 • Sar1 Val5 Ala8 Angiotensin II • Biotin • Pneumadin human AA Ala Gly Glu Pro Lys Leu Asp Ala • Sar1 Angiotensin I II 1 7 amide • Atrial Natriuretic Factor 1 29 chicken AA Met Met Arg Asp Gly Val NH2 MW 955 08 • Val4 Angiotensin III Ser Gly Cys Phe Gly Arg Arg Ile Asp Arg Ile Gly Ser Leu Ser • Pneumadin rat AA Tyr Gly Glu Pro Lys Leu Asp Ala Gly Val • Brain Injury Derived Neurotrophic Peptide AA Glu Ala Leu Gly Met Gly Cys Asn Gly Ser Arg Lys Asn Disulfide bridge NH2 MW 1047 18 Glu Leu Ala Arg Gly Ala Ile Phe Gln Ala NH2 MW 1387 61 Cys7 Cys23 MW • Osteostatin amide human AA Thr Arg Ser Ala Trp Leu Asp • Neurotrophic Factor for Retinal Cholinergic Neurons AA • Atrial Natriuretic Factor 3 28 human AA Arg Arg Ser Ser Ser Gly Val Thr Gly Ser Gly Leu Glu Gly Asp His Leu Ser Tyr Leu Leu Pro Ala Gln Val Asn Ile Asp MW 1145 33 Cys Phe Gly Gly Arg Met Asp Arg Ile Gly Ala Gln Ser Gly Asp Thr Ser Thr Thr Ser Leu Glu Leu Asp Ser Arg NH2 MW • Biotin Obestatin human AA Biotin Phe Asn Ala Pro Phe Leu Gly Cys Asn Ser Phe Arg Tyr Disulfide bridge Cys5 3450 64 Asp Val Gly Ile Lys Leu Ser Gly Val Gln Tyr Gln Gln His Ser Cys21 MW 2880 3 • Osteostatin -
Table of Contents
Table of Contents Table of Contents 4 Educational Resources 5 Payment Options 7 Getting Started With Our Lab Services 8 Frequently Asked Questions 9 Testimonials 10 Tests 11 Organic Acids Test (OAT) 12 Sample Test 13 Report Analysis 14 IgG Food Allergy Test w/ Candida 15 Sample Report 16 Metals Tests 17 Gluten / Casein Peptides Test 19 IgE Food and Inhalant Allergy Tests 20 Hormone Panels 21 Amino Acids Tests 22 Vitamin D Test 23 Advanced Cholesterol Profile 24 Immune Deficiency Profile 25 Comprehensive Stool Analysis 26 Copper / Zinc Profile 27 Comprehensive Fatty Acids Test 28 GPL-3 29 GPL-4 30 OAT + IgG Food Allergy Test w/ Candida Combo 31 Comprehensive Test Panels 32 Comprehensive Autism Panel 32 Comprehensive AD(H)D Panel 32 Comprehensive Mental Health Panel 32 Comprehensive Wellness Panel 32 The Great Plains Laboratory, Inc. Information Guide A research-based clinical laboratory offering services worldwide. 1 William Shaw, Ph.D., Director | 11813 West 77th Street, Lenexa, KS 66214 | (913) 341-8949 | Fax (913) 341-6207call: | (913) www.GPL4U.com 341-8949 The Great Plains Laboratory, Inc. (GPL) is a research-based clinical laboratory that offers testing for nutritional factors in chronic illnesses worldwide. Our company was founded in 1996 and is currently serving more than 100 countries. We provide a variety of metabolic tests that are not routinely available through other laboratories, and have tested more than 200,000 patients with autism and other related disorders. Our goal is to help people achieve their maximum potential through quality laboratory testing, knowledgeable staff, and excellent customer service. -
Genscript Product Catalog 2013-2014 Genscript Product Catalog
GenScript Product Catalog 2013-2014 GenScript Product Catalog www.genscript.com GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ 08854USA Tel: 1-732-885-9188 / 1-732-885-9688 Toll-Free Tel: 1-877-436-7274 Fax: 1-732-210-0262 / 1-732-885-5878 Email: [email protected] Nucleic Acid Purification and Analysis Business Development Tel: 1-732-317-5088 PCR PCR and Cloning Email: [email protected] Protein Analysis Antibodies 2013-2014 Peptides Welcome to GenScript GenScript USA Incorporation, founded in 2002, is a fast-growing biotechnology company and contract research organization (CRO) specialized in custom services and consumable products for academic and pharmaceutical research. Built on our assembly-line mode, one-stop solutions, continuous improvement, and stringent IP protection, GenScript provides a comprehensive portfolio of products and services at the most competitive prices in the industry to meet your research needs every day. Over the years, GenScript’s scientists have developed many innovative technologies that allow us to maintain our position at the cutting edge of biological and medical research while offering cost-effective solutions for customers to accelerate their research. Our advanced expertise includes proprietary technology for custom gene synthesis, OptimumGeneTM codon optimization technology, CloneEZ® seamless cloning technology, FlexPeptideTM technology for custom peptide synthesis, BacPowerTM technology for protein expression and purification, T-MaxTM adjuvant and advanced nanotechnology for custom antibody production, as well as our ONE-HOUR WesternTM detection system and eStain® protein staining system. GenScript offers a broad range of reagents, optimized kits, and system solutions to help you unravel the mysteries of biology. We also provide a comprehensive portfolio of customized services that include Bio-Reagent, Bio-Assay, Lead Optimization, and Antibody Drug Development which can be effectively integrated into your value chain and your operations. -
Sized Neuropeptides
M ETHODS IN MOLECULAR BIOLOGY™ Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK For further volumes: http://www.springer.com/series/7651 Neuropeptides Methods and Protocols Edited by Adalberto Merighi Dipartimento di Morfofisiologia Veterinaria, Università degli Studi di Torino, Grugliasco, TO, Italy; Istituto Nazionale di Neuroscienze (INN), Università degli Studi di Torino, Grugliasco, TO, Italy Editor Adalberto Merighi Dipartimento di Morfofisiologia Veterinaria Università degli Studi di Torino and Istituto Nazionale di Neuroscienze (INN) Università degli Studi di Torino Grugliasco, TO, Italy [email protected] Please note that additional material for this book can be downloaded from http://extras.springer.com ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-309-7 e-ISBN 978-1-61779-310-3 DOI 10.1007/978-1-61779-310-3 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011936011 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. -
(12) Patent Application Publication (10) Pub. No.: US 2014/0144429 A1 Wensley Et Al
US 2014O144429A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2014/0144429 A1 Wensley et al. (43) Pub. Date: May 29, 2014 (54) METHODS AND DEVICES FOR COMPOUND (60) Provisional application No. 61/887,045, filed on Oct. DELIVERY 4, 2013, provisional application No. 61/831,992, filed on Jun. 6, 2013, provisional application No. 61/794, (71) Applicant: E-NICOTINE TECHNOLOGY, INC., 601, filed on Mar. 15, 2013, provisional application Draper, UT (US) No. 61/730,738, filed on Nov. 28, 2012. (72) Inventors: Martin Wensley, Los Gatos, CA (US); Publication Classification Michael Hufford, Chapel Hill, NC (US); Jeffrey Williams, Draper, UT (51) Int. Cl. (US); Peter Lloyd, Walnut Creek, CA A6M II/04 (2006.01) (US) (52) U.S. Cl. CPC ................................... A6M II/04 (2013.O1 (73) Assignee: E-NICOTINE TECHNOLOGY, INC., ( ) Draper, UT (US) USPC ..................................................... 128/200.14 (21) Appl. No.: 14/168,338 (57) ABSTRACT 1-1. Provided herein are methods, devices, systems, and computer (22) Filed: Jan. 30, 2014 readable medium for delivering one or more compounds to a O O Subject. Also described herein are methods, devices, systems, Related U.S. Application Data and computer readable medium for transitioning a Smoker to (63) Continuation of application No. PCT/US 13/72426, an electronic nicotine delivery device and for Smoking or filed on Nov. 27, 2013. nicotine cessation. Patent Application Publication May 29, 2014 Sheet 1 of 26 US 2014/O144429 A1 FIG. 2A 204 -1 2O6 Patent Application Publication May 29, 2014 Sheet 2 of 26 US 2014/O144429 A1 Area liquid is vaporized Electrical Connection Agent O s 2. -
Lightning Thief Chapter Summaries Book Summaries
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Opioid Peptides in Peripheral Pain Control
Review Acta Neurobiol Exp 2011, 71: 129–138 Opioid peptides in peripheral pain control Anna Lesniak*, Andrzej W. Lipkowski Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw; *Email: [email protected] Opioids have a long history of therapeutic use as a remedy for various pain states ranging from mild acute nociceptive pain to unbearable chronic advanced or end-stage disease pain. Analgesia produced by classical opioids is mediated extensively by binding to opioid receptors located in the brain or the spinal cord. Nevertheless, opioid receptors are also expressed outside the CNS in the periphery and may become valuable assets in eliciting analgesia devoid of shortcomings typical for the activation of their central counterparts. The discovery of endogenous opioid peptides that participate in the formation, transmission, modulation and perception of pain signals offers numerous opportunities for the development of new analgesics. Novel peptidic opioid receptor analogs, which show limited access through the blood brain barrier may support pain therapy requiring prolonged use of opioid drugs. Key words: immune cells, opioid peptides, pain, peripheral analgesia Abbreviations: β-FNA - β-funaltrexamine, BBB - blood-brain-barrier, CGRP - calcitonin gene-related peptide, CFA - complete Freund adjuvant, CNS - central nervous system, CRF - corticotropin releasing factor, CYP - cyprodime, DAGO - [Tyr-D-Ala- Gly-Me-Phe-Gly-ol]-enkephalin, DAMGO - [D-Ala2, N-MePhe4, Gly-ol]-enkephalin, DOR - delta opioid receptor, DPDPE - [D-Pen2,5]-enkephalin, DRG - dorsal root ganglion, EM-1 - endomorphin 1, EM-2 - endomorphin 2, KOR - kappa opioid receptor, MOR - mu opioid receptor, NLZ – naloxonazine, NTI - naltrindole, NLXM - naloxone methiodide; nor-BNI – nor-binaltorphimine, PDYN - prodynorphin, PENK - proenkephalin, PNS - peripheral nervous system, POMC - proopiomelanocortin INTRODUCTION ic pain. -
Five Decades of Research on Opioid Peptides: Current Knowledge and Unanswered Questions
Molecular Pharmacology Fast Forward. Published on June 2, 2020 as DOI: 10.1124/mol.120.119388 This article has not been copyedited and formatted. The final version may differ from this version. File name: Opioid peptides v45 Date: 5/28/20 Review for Mol Pharm Special Issue celebrating 50 years of INRC Five decades of research on opioid peptides: Current knowledge and unanswered questions Lloyd D. Fricker1, Elyssa B. Margolis2, Ivone Gomes3, Lakshmi A. Devi3 1Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; E-mail: [email protected] 2Department of Neurology, UCSF Weill Institute for Neurosciences, 675 Nelson Rising Lane, San Francisco, CA 94143, USA; E-mail: [email protected] 3Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, Annenberg Downloaded from Building, One Gustave L. Levy Place, New York, NY 10029, USA; E-mail: [email protected] Running Title: Opioid peptides molpharm.aspetjournals.org Contact info for corresponding author(s): Lloyd Fricker, Ph.D. Department of Molecular Pharmacology Albert Einstein College of Medicine 1300 Morris Park Ave Bronx, NY 10461 Office: 718-430-4225 FAX: 718-430-8922 at ASPET Journals on October 1, 2021 Email: [email protected] Footnotes: The writing of the manuscript was funded in part by NIH grants DA008863 and NS026880 (to LAD) and AA026609 (to EBM). List of nonstandard abbreviations: ACTH Adrenocorticotrophic hormone AgRP Agouti-related peptide (AgRP) α-MSH Alpha-melanocyte stimulating hormone CART Cocaine- and amphetamine-regulated transcript CLIP Corticotropin-like intermediate lobe peptide DAMGO D-Ala2, N-MePhe4, Gly-ol]-enkephalin DOR Delta opioid receptor DPDPE [D-Pen2,D- Pen5]-enkephalin KOR Kappa opioid receptor MOR Mu opioid receptor PDYN Prodynorphin PENK Proenkephalin PET Positron-emission tomography PNOC Pronociceptin POMC Proopiomelanocortin 1 Molecular Pharmacology Fast Forward.